Restriction Fragments for YDRCsigma4



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The currently selected gene/sequence is :

YDRCsigma4 on chromosome IV from coordinates 992815 to 992740.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.31271.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 14 + TaqI TCGA 11 13 . . 23 26 + SetI ASST 26 22 . . 24 27 + MnlI CCTC 34 33 . . 33 43 + Hin4I GAYNNNNNVTC 24 19 56 51 33 43 - Hin4I GAYNNNNNVTC 56 51 24 19 43 46 + FatI CATG 42 46 . . 43 46 + NlaIII CATG 46 42 . . 43 46 + CviAII CATG 43 45 . . 54 58 - TspDTI ATGAA 44 42 . . 65 70 + NdeI CATATG 66 68 . . #--------------------------------------- # # # CviAII: # [CATG] # 33 43 # # FatI: # [CATG] # 34 42 # # Hin4I: # [GAYNNNNNVTC] # 0 0 20 24 32 # # MnlI: # [CCTC] # 34 42 # # NdeI: # [CATATG] # 10 66 # # NlaIII: # [CATG] # 30 46 # # SetI: # [ASST] # 26 50 # # TaqI: # [TCGA] # 11 65 # # TspDTI: # [ATGAA] # 32 44 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 76 # Reported_sequences: 1 # Reported_hitcount: 10 #---------------------------------------