Restriction Fragments for APT2/YDR441C



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The currently selected gene/sequence is :

APT2/YDR441C on chromosome IV from coordinates 1345062 to 1344517.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14754.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 14 + Hpy188I TCNGA 12 11 . . 13 17 + TfiI GAWTC 13 16 . . 13 17 + HinfI GANTC 13 16 . . 39 42 + CviRI* TGCA 40 40 . . 51 56 + Hpy166II GTNNAC 53 53 . . 54 65 + TstI CACNNNNNNTCC 45 40 77 72 54 60 + TspRI CASTGNN 60 51 . . 64 74 + BsiYI* CCNNNNNNNGG 70 67 . . 70 73 - MnlI CCTC 63 62 . . 73 77 + HphI GGTGA 85 84 . . 80 83 + TspEI AATT 79 83 . . 85 88 - MnlI CCTC 78 77 . . 96 99 + SetI ASST 99 95 . . 97 107 + BsiYI* CCNNNNNNNGG 103 100 . . 123 133 + BsiYI* CCNNNNNNNGG 129 126 . . 123 133 + PflMI CCANNNNNTGG 129 126 . . 133 138 + Hpy166II GTNNAC 135 135 . . 133 136 + Csp6I GTAC 133 135 . . 133 136 + RsaI GTAC 134 134 . . 137 142 + AflIII ACRYGT 137 141 . . 139 142 + TaiI ACGT 142 138 . . 139 142 + SetI ASST 142 138 . . 139 142 + MaeII ACGT 139 141 . . 132 142 - BaeI ACNNNNGTAYC 157 152 124 119 143 148 + Hpy178III* TCNNGA 144 146 . . 147 151 + HgaI GACGC 156 161 . . 157 161 + MboII GAAGA 169 168 . . 178 188 + FalI AAGNNNNNCTT 169 164 201 196 180 183 + DpnI GATC 181 181 . . 180 183 + BstKTI GATC 182 180 . . 180 183 + MboI GATC 179 183 . . 182 185 + TaqI TCGA 182 184 . . 183 194 + BcgI CGANNNNNNTGC 172 170 206 204 178 188 - FalI AAGNNNNNCTT 201 196 169 164 192 195 + CviRI* TGCA 193 193 . . 195 198 + SetI ASST 198 194 . . 193 198 - BspMI ACCTGC 184 188 . . 204 207 + SetI ASST 207 203 . . 204 207 + CviJI RGCY 205 205 . . 204 207 + AluI AGCT 205 205 . . 206 209 + MaeI CTAG 206 208 . . 210 215 + StuI AGGCCT 212 212 . . 211 214 + CviJI RGCY 212 212 . . 211 214 + HaeIII GGCC 212 212 . . 209 212 - MnlI CCTC 202 201 . . 213 217 + Hin4II* CCTTC 223 222 . . 223 227 + BmgT120I GGNCC 224 225 . . 223 227 + AsuI* GGNCC 223 226 . . 224 227 + CviJI RGCY 225 225 . . 224 227 + HaeIII GGCC 225 225 . . 226 230 + BccI CCATC 234 235 . . 227 231 - FokI GGATG 213 217 . . 227 231 - BseGI GGATG 226 224 . . 231 241 + BsiYI* CCNNNNNNNGG 237 234 . . 232 235 + MaeI CTAG 232 234 . . 236 242 + TspRI CASTGNN 242 233 . . 237 241 + BsrI ACTGG 242 240 . . 250 255 - MmeI TCCRAC 231 229 . . 274 279 - MmeI TCCRAC 255 253 . . 281 284 + TspEI AATT 280 284 . . 286 290 + BssKI CCNGG 285 290 . . 286 289 + HpaII CCGG 286 288 . . 286 290 + ScrFI CCNGG 287 288 . . 286 290 + CauII* CCSGG 287 288 . . 289 293 + HphI GGTGA 301 300 . . 317 320 + TspEI AATT 316 320 . . 325 328 + FatI CATG 324 328 . . 325 328 + NlaIII CATG 328 324 . . 325 328 + CviAII CATG 325 327 . . 326 330 + TspDTI ATGAA 341 339 . . 328 332 + MboII GAAGA 340 339 . . 332 337 + ApoI RAATTY 332 336 . . 333 336 + TspEI AATT 332 336 . . 344 347 + CviRI* TGCA 345 345 . . 347 350 + SetI ASST 350 346 . . 345 350 - BspMI ACCTGC 336 340 . . 352 361 + XmnI GAANNNNTTC 356 356 . . 354 357 + CviJI RGCY 355 355 . . 367 371 + TfiI GAWTC 367 370 . . 367 371 + HinfI GANTC 367 370 . . 370 375 + MmeI TCCRAC 395 393 . . 374 377 + TaiI ACGT 377 373 . . 374 377 + SetI ASST 377 373 . . 374 377 + MaeII ACGT 374 376 . . 387 391 + FokI GGATG 400 404 . . 387 391 + BseGI GGATG 393 391 . . 402 409 + SgrAI CRCCGGYG 403 407 . . 403 408 + Cfr10I RCCGGY 403 407 . . 403 408 + BetI* WCCGGW 403 407 . . 403 408 + AgeI ACCGGT 403 407 . . 404 407 + HpaII CCGG 404 406 . . 412 416 + BceAI ACGGC 428 430 . . 415 425 + MwoI GCNNNNNNNGC 421 418 . . 415 425 + BstAPI GCANNNNNTGC 421 418 . . 416 421 + NdeI CATATG 417 419 . . 421 425 + BlsI GCNGC 423 422 . . 421 425 + BisI GCNGC 422 423 . . 421 425 + TseI GCWGC 421 424 . . 424 429 + Cac8I GCNNGC 426 426 . . 421 425 - BbvI GCAGC 408 412 . . 424 427 - AciI CCGC 424 426 . . 424 428 - FauI CCCGC 417 419 . . 431 434 + SetI ASST 434 430 . . 437 441 + Hpy188I TCNGA 439 438 . . 449 454 + SduI GDGCHC 453 449 . . 449 454 + HgiAI* GWGCWC 453 449 . . 446 451 - TaqII CACCCA 436 434 . . 459 464 + Hpy178III* TCNNGA 460 462 . . 478 481 + MaeI CTAG 478 480 . . 491 496 + Hpy166II GTNNAC 493 493 . . 491 496 + AccI GTMKAC 492 494 . . 496 499 + FatI CATG 495 499 . . 496 499 + NlaIII CATG 499 495 . . 496 499 + CviAII CATG 496 498 . . 499 503 + HphI GGTGA 511 510 . . 502 507 + BseRI GAGGAG 517 515 . . 502 505 - MnlI CCTC 495 494 . . 509 512 + TspEI AATT 508 512 . . 505 515 - Hin4I GAYNNNNNVTC 528 523 496 491 536 539 + CviRI* TGCA 537 537 . . 541 546 + Hpy178III* TCNNGA 542 544 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 54 492 # # AciI: # [CCGC] # 122 424 # # AflIII: # [ACRYGT] # 137 409 # # AgeI: # [ACCGGT] # 143 403 # # AluI: # [AGCT] # 205 341 # # ApoI: # [RAATTY] # 214 332 # # AsuI*: # [GGNCC] # 223 323 # # BaeI: # [ACNNNNGTAYC] # 33 124 389 # # BbvI: # [GCAGC] # 138 408 # # BccI: # [CCATC] # 234 312 # # BceAI: # [ACGGC] # 118 428 # # BcgI: # [CGANNNNNNTGC] # 34 172 340 # # BetI*: # [WCCGGW] # 143 403 # # BisI: # [GCNGC] # 124 422 # # BlsI: # [GCNGC] # 123 423 # # BmgT120I: # [GGNCC] # 224 322 # # BseGI: # [GGATG] # 153 167 226 # # BseRI: # [GAGGAG] # 29 517 # # BsiYI*: # [CCNNNNNNNGG] # 26 33 70 108 309 # # BspMI: # [ACCTGC] # 152 184 210 # # BsrI: # [ACTGG] # 242 304 # # BssKI: # [CCNGG] # 261 285 # # BstAPI: # [GCANNNNNTGC] # 125 421 # # BstKTI: # [GATC] # 182 364 # # Cac8I: # [GCNNGC] # 120 426 # # CauII*: # [CCSGG] # 259 287 # # Cfr10I: # [RCCGGY] # 143 403 # # Csp6I: # [GTAC] # 133 413 # # CviAII: # [CATG] # 50 171 325 # # CviJI: # [RGCY] # 7 13 130 191 205 # # CviRI*: # [TGCA] # 9 40 152 153 192 # # DpnI: # [GATC] # 181 365 # # FalI: # [AAGNNNNNCTT] # 0 0 32 169 345 # # FatI: # [CATG] # 51 171 324 # # FauI: # [CCCGC] # 129 417 # # FokI: # [GGATG] # 146 187 213 # # HaeIII: # [GGCC] # 13 212 321 # # HgaI: # [GACGC] # 156 390 # # HgiAI*: # [GWGCWC] # 93 453 # # Hin4I: # [GAYNNNNNVTC] # 18 32 496 # # Hin4II*: # [CCTTC] # 223 323 # # HinfI: # [GANTC] # 13 179 354 # # HpaII: # [CCGG] # 118 142 286 # # HphI: # [GGTGA] # 35 85 210 216 # # Hpy166II: # [GTNNAC] # 53 53 82 358 # # Hpy178III*: # [TCNNGA] # 4 82 144 316 # # Hpy188I: # [TCNGA] # 12 107 427 # # MaeI: # [CTAG] # 26 68 206 246 # # MaeII: # [ACGT] # 139 172 235 # # MboI: # [GATC] # 179 367 # # MboII: # [GAAGA] # 169 171 206 # # MmeI: # [TCCRAC] # 24 140 151 231 # # MnlI: # [CCTC] # 15 51 63 124 293 # # MwoI: # [GCNNNNNNNGC] # 125 421 # # NdeI: # [CATATG] # 129 417 # # NlaIII: # [CATG] # 47 171 328 # # PflMI: # [CCANNNNNTGG] # 129 417 # # RsaI: # [GTAC] # 134 412 # # ScrFI: # [CCNGG] # 259 287 # # SduI: # [GDGCHC] # 93 453 # # SetI: # [ASST] # 9 27 43 56 57 99 # 112 143 # # SgrAI: # [CRCCGGYG] # 143 403 # # StuI: # [AGGCCT] # 212 334 # # TaiI: # [ACGT] # 142 169 235 # # TaqI: # [TCGA] # 182 364 # # TaqII: # [CACCCA] # 110 436 # # TfiI: # [GAWTC] # 13 179 354 # # TseI: # [GCWGC] # 125 421 # # TspDTI: # [ATGAA] # 205 341 # # TspEI: # [AATT] # 16 36 38 79 176 201 # # TspRI: # [CASTGNN] # 60 182 304 # # TstI: # [CACNNNNNNTCC] # 32 45 469 # # XmnI: # [GAANNNNTTC] # 190 356 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 546 # Reported_sequences: 1 # Reported_hitcount: 121 #---------------------------------------