Restriction Fragments for SPC110/YDR356W



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The currently selected gene/sequence is :

SPC110/YDR356W on chromosome IV from coordinates 1186107 to 1188941.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27941.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 14 + Eam1105I GACNNNNNGTC 9 8 . . 12 16 + Tsp45I GTSAC 11 16 . . 12 16 + MaeIII GTNAC 11 16 . . 4 14 - Hin4I GAYNNNNNVTC 27 22 . . 31 34 + SetI ASST 34 30 . . 31 34 + CviJI RGCY 32 32 . . 31 34 + AluI AGCT 32 32 . . 36 40 + MboII GAAGA 48 47 . . 42 45 + FatI CATG 41 45 . . 42 45 + NlaIII CATG 45 41 . . 42 45 + CviAII CATG 42 44 . . 46 51 + ApoI RAATTY 46 50 . . 47 50 + TspEI AATT 46 50 . . 56 61 + SfeI* CTRYAG 56 60 . . 113 117 + DdeI CTNAG 113 116 . . 116 119 + SetI ASST 119 115 . . 117 122 + NlaIV GGNNCC 119 119 . . 136 140 + HphI GGTGA 148 147 . . 150 156 + DraII RGGNCCY 151 154 . . 151 154 + CviJI RGCY 152 152 . . 151 154 + HaeIII GGCC 152 152 . . 151 155 + BmgT120I GGNCC 152 153 . . 151 155 + AsuI* GGNCC 151 154 . . 148 152 - Hin4II* CCTTC 142 141 . . 158 161 + MseI TTAA 158 160 . . 173 177 + MboII GAAGA 185 184 . . 191 196 + MfeI CAATTG 191 195 . . 192 195 + TspEI AATT 191 195 . . 196 200 + HinfI GANTC 196 199 . . 196 200 - PleI GAGTC 190 191 . . 196 200 - MlyI GAGTC 190 190 . . 206 209 + CviJI RGCY 207 207 . . 218 223 + HindIII AAGCTT 218 222 . . 219 222 + SetI ASST 222 218 . . 219 222 + CviJI RGCY 220 220 . . 219 222 + AluI AGCT 220 220 . . 227 230 + TspEI AATT 226 230 . . 230 233 + TaqI TCGA 230 232 . . 238 241 + SetI ASST 241 237 . . 238 241 + CviJI RGCY 239 239 . . 238 241 + AluI AGCT 239 239 . . 243 248 + Hpy178III* TCNNGA 244 246 . . 249 252 + TspEI AATT 248 252 . . 251 254 + MseI TTAA 251 253 . . 255 258 + CviJI RGCY 256 256 . . 265 268 + AciI CCGC 265 267 . . 264 269 - EciI GGCGGA 254 252 . . 269 272 + MaeI CTAG 269 271 . . 274 279 + StyI CCWWGG 274 278 . . 274 279 + SecI* CCNNGG 274 278 . . 274 284 + BsiYI* CCNNNNNNNGG 280 277 . . 277 280 + SetI ASST 280 276 . . 284 289 + BsrDI GCAATG 291 289 . . 289 294 + Hpy166II GTNNAC 291 291 . . 289 294 + HindII GTYRAC 291 291 . . 292 301 + PshAI GACNNNNGTC 296 296 . . 309 314 + ApoI RAATTY 309 313 . . 310 313 + TspEI AATT 309 313 . . 327 331 + MboII GAAGA 339 338 . . 345 349 + BsmAI GTCTC 350 354 . . 347 351 + DdeI CTNAG 347 350 . . 347 351 + BspCNI CTCAG 360 358 . . 347 351 + BseMII CTCAG 361 359 . . 350 353 + CviJI RGCY 351 351 . . 385 389 + TspDTI ATGAA 400 398 . . 387 391 + MboII GAAGA 399 398 . . 404 408 + Tsp4CI* ACNGT 406 405 . . 422 425 + TspEI AATT 421 425 . . 427 430 + MaeI CTAG 427 429 . . 453 458 + ApoI RAATTY 453 457 . . 454 457 + TspEI AATT 453 457 . . 457 461 + Hpy188I TCNGA 459 458 . . 484 489 + VspI ATTAAT 485 487 . . 485 488 + MseI TTAA 485 487 . . 488 492 + TspDTI ATGAA 503 501 . . 494 499 + Hpy178III* TCNNGA 495 497 . . 508 511 + FatI CATG 507 511 . . 508 511 + NlaIII CATG 511 507 . . 508 511 + CviAII CATG 508 510 . . 509 513 + TspDTI ATGAA 524 522 . . 521 524 + TspEI AATT 520 524 . . 523 526 + MseI TTAA 523 525 . . 553 556 + TspEI AATT 552 556 . . 568 572 + MboII GAAGA 580 579 . . 571 575 + MboII GAAGA 583 582 . . 588 592 + MboII GAAGA 600 599 . . 603 608 + HaeII RGCGCY 607 603 . . 603 608 + Eco47III AGCGCT 605 605 . . 604 607 + Hin6I GCGC 604 606 . . 604 607 + GlaI GCGC 605 605 . . 604 607 + HhaI GCGC 606 604 . . 617 620 + SetI ASST 620 616 . . 617 620 + CviJI RGCY 618 618 . . 617 620 + AluI AGCT 618 618 . . 622 626 + HgaI GACGC 631 636 . . 622 627 + AcyI GRCGYC 623 625 . . 636 639 + TspEI AATT 635 639 . . 642 647 + EcoRI GAATTC 642 646 . . 642 647 + ApoI RAATTY 642 646 . . 643 646 + TspEI AATT 642 646 . . 646 651 + Hpy178III* TCNNGA 647 649 . . 660 665 + BclI TGATCA 660 664 . . 661 664 + DpnI GATC 662 662 . . 661 664 + BstKTI GATC 663 661 . . 661 664 + MboI GATC 660 664 . . 667 672 + Hpy178III* TCNNGA 668 670 . . 670 674 + FokI GGATG 683 687 . . 670 674 + BseGI GGATG 676 674 . . 672 677 + EcoT22I ATGCAT 676 672 . . 673 676 + CviRI* TGCA 674 674 . . 671 675 - SfaNI GCATC 661 665 . . 679 683 + MboII GAAGA 691 690 . . 684 687 + TaiI ACGT 687 683 . . 684 687 + SetI ASST 687 683 . . 684 687 + MaeII ACGT 684 686 . . 686 691 + Hpy166II GTNNAC 688 688 . . 708 711 + CviJI RGCY 709 709 . . 713 716 + TspEI AATT 712 716 . . 733 737 + Tsp4CI* ACNGT 735 734 . . 751 754 + MaeI CTAG 751 753 . . 755 758 + TspEI AATT 754 758 . . 770 774 + Tsp45I GTSAC 769 774 . . 770 774 + MaeIII GTNAC 769 774 . . 773 776 + TaiI ACGT 776 772 . . 773 776 + SetI ASST 776 772 . . 773 776 + MaeII ACGT 773 775 . . 775 778 + Csp6I GTAC 775 777 . . 775 778 + RsaI GTAC 776 776 . . 783 788 + AhaIII* TTTAAA 785 785 . . 784 787 + MseI TTAA 784 786 . . 788 791 + TspEI AATT 787 791 . . 793 798 + XhoII RGATCY 793 797 . . 793 798 + BglII AGATCT 793 797 . . 794 797 + DpnI GATC 795 795 . . 794 797 + BstKTI GATC 796 794 . . 794 797 + MboI GATC 793 797 . . 797 801 + DdeI CTNAG 797 800 . . 804 808 + FokI GGATG 817 821 . . 804 808 + BseGI GGATG 810 808 . . 802 805 - MnlI CCTC 795 794 . . 806 810 + TspDTI ATGAA 821 819 . . 809 812 + TspEI AATT 808 812 . . 813 817 + MboII GAAGA 825 824 . . 816 821 + ApoI RAATTY 816 820 . . 817 820 + TspEI AATT 816 820 . . 820 823 + MseI TTAA 820 822 . . 823 827 + TspDTI ATGAA 838 836 . . 836 840 + TspDTI ATGAA 851 849 . . 839 842 + TspEI AATT 838 842 . . 843 847 + MboII GAAGA 855 854 . . 843 848 - Ksp632I* CTCTTC 838 841 . . 848 853 + BsrDI GCAATG 855 853 . . 843 849 - SapI GCTCTTC 838 841 . . 852 855 + CviRI* TGCA 853 853 . . 856 860 + MboII GAAGA 868 867 . . 864 869 - BciVI GTATCC 858 857 . . 870 874 + Tsp4CI* ACNGT 872 871 . . 877 882 + EcoRI GAATTC 877 881 . . 877 882 + ApoI RAATTY 877 881 . . 878 881 + TspEI AATT 877 881 . . 873 878 - MmeI TCCRAC 854 852 . . 881 886 + Hpy178III* TCNNGA 882 884 . . 890 894 + TspDTI ATGAA 905 903 . . 895 899 + DdeI CTNAG 895 898 . . 895 899 - BspCNI CTCAG 887 885 . . 895 899 - BseMII CTCAG 886 884 . . 897 900 - MnlI CCTC 890 889 . . 911 915 + TspDTI ATGAA 926 924 . . 914 917 + TspEI AATT 913 917 . . 916 919 + MseI TTAA 916 918 . . 937 941 + Hpy188I TCNGA 939 938 . . 945 949 - BccI CCATC 939 940 . . 974 978 + TspDTI ATGAA 989 987 . . 976 980 + TfiI GAWTC 976 979 . . 976 980 + HinfI GANTC 976 979 . . 988 991 + MseI TTAA 988 990 . . 995 999 + TspDTI ATGAA 1010 1008 . . 998 1001 + TspEI AATT 997 1001 . . 1000 1003 + MseI TTAA 1000 1002 . . 1007 1010 + SetI ASST 1010 1006 . . 1007 1010 + CviJI RGCY 1008 1008 . . 1007 1010 + AluI AGCT 1008 1008 . . 1018 1023 + ApoI RAATTY 1018 1022 . . 1019 1022 + TspEI AATT 1018 1022 . . 1037 1041 + DdeI CTNAG 1037 1040 . . 1037 1041 + BspCNI CTCAG 1050 1048 . . 1037 1041 + BseMII CTCAG 1051 1049 . . 1042 1046 - MboII GAAGA 1034 1033 . . 1041 1046 - BbvII* GAAGAC 1034 1038 . . 1047 1050 + CviRI* TGCA 1048 1048 . . 1058 1062 + TspDTI ATGAA 1073 1071 . . 1061 1064 + TspEI AATT 1060 1064 . . 1086 1089 + CviJI RGCY 1087 1087 . . 1087 1092 + NmeAIII GCCGAG 1113 1111 . . 1091 1094 + SetI ASST 1094 1090 . . 1091 1094 + CviJI RGCY 1092 1092 . . 1091 1094 + AluI AGCT 1092 1092 . . 1093 1096 + MaeI CTAG 1093 1095 . . 1096 1099 - MnlI CCTC 1089 1088 . . 1099 1103 + MboII GAAGA 1111 1110 . . 1099 1104 - Ksp632I* CTCTTC 1094 1097 . . 1116 1121 + ApoI RAATTY 1116 1120 . . 1117 1120 + TspEI AATT 1116 1120 . . 1122 1126 + Tsp4CI* ACNGT 1124 1123 . . 1126 1129 + MseI TTAA 1126 1128 . . 1140 1143 + SetI ASST 1143 1139 . . 1138 1142 - Hin4II* CCTTC 1132 1131 . . 1150 1154 + SfaNI GCATC 1159 1163 . . 1155 1160 + VspI ATTAAT 1156 1158 . . 1156 1159 + MseI TTAA 1156 1158 . . 1161 1164 + CviJI RGCY 1162 1162 . . 1162 1168 + EspI* GCTNAGC 1163 1166 . . 1163 1167 + DdeI CTNAG 1163 1166 . . 1163 1167 + BspCNI CTCAG 1176 1174 . . 1163 1167 + BseMII CTCAG 1177 1175 . . 1166 1169 + SetI ASST 1169 1165 . . 1166 1169 + CviJI RGCY 1167 1167 . . 1166 1169 + AluI AGCT 1167 1167 . . 1174 1179 + MfeI CAATTG 1174 1178 . . 1175 1178 + TspEI AATT 1174 1178 . . 1184 1189 + Hpy166II GTNNAC 1186 1186 . . 1190 1193 + MseI TTAA 1190 1192 . . 1201 1205 + HinfI GANTC 1201 1204 . . 1199 1203 - BsmAI GTCTC 1193 1197 . . 1201 1205 - PleI GAGTC 1195 1196 . . 1201 1205 - MlyI GAGTC 1195 1195 . . 1206 1210 - BsrI ACTGG 1206 1204 . . 1205 1210 - BfiI ACTGGG 1200 1199 . . 1212 1217 + SduI GDGCHC 1216 1212 . . 1212 1217 + HgiJII* GRGCYC 1216 1212 . . 1213 1216 + CviJI RGCY 1214 1214 . . 1215 1220 + SmlI CTYRAG 1215 1219 . . 1215 1220 - Bce83I* CTTGAG 1200 1198 . . 1222 1226 + MboII GAAGA 1234 1233 . . 1225 1229 + MboII GAAGA 1237 1236 . . 1229 1232 + TspEI AATT 1228 1232 . . 1250 1253 + SetI ASST 1253 1249 . . 1250 1253 + CviJI RGCY 1251 1251 . . 1250 1253 + AluI AGCT 1251 1251 . . 1261 1266 + EcoRV GATATC 1263 1263 . . 1265 1270 + Hpy178III* TCNNGA 1266 1268 . . 1279 1282 - MnlI CCTC 1272 1271 . . 1285 1289 + Tsp4CI* ACNGT 1287 1286 . . 1296 1300 + FokI GGATG 1309 1313 . . 1296 1300 + BseGI GGATG 1302 1300 . . 1298 1302 + TspDTI ATGAA 1313 1311 . . 1302 1306 + TspGWI ACGGA 1317 1315 . . 1305 1315 + Hin4I GAYNNNNNVTC 1296 1291 1328 1323 1308 1311 + TspEI AATT 1307 1311 . . 1312 1315 + DpnI GATC 1313 1313 . . 1312 1315 + BstKTI GATC 1314 1312 . . 1312 1315 + MboI GATC 1311 1315 . . 1305 1315 - Hin4I GAYNNNNNVTC 1328 1323 1296 1291 1314 1318 - MboII GAAGA 1306 1305 . . 1325 1328 + SetI ASST 1328 1324 . . 1328 1331 + MseI TTAA 1328 1330 . . 1334 1337 + SetI ASST 1337 1333 . . 1334 1337 + CviJI RGCY 1335 1335 . . 1334 1337 + AluI AGCT 1335 1335 . . 1336 1339 + MaeI CTAG 1336 1338 . . 1370 1374 + Tsp4CI* ACNGT 1372 1371 . . 1371 1377 + TspRI CASTGNN 1377 1368 . . 1375 1379 + PleI GAGTC 1383 1384 . . 1375 1379 + MlyI GAGTC 1384 1384 . . 1375 1379 + HinfI GANTC 1375 1378 . . 1397 1401 + TspDTI ATGAA 1412 1410 . . 1402 1405 + MaeI CTAG 1402 1404 . . 1405 1409 + TfiI GAWTC 1405 1408 . . 1405 1409 + HinfI GANTC 1405 1408 . . 1412 1422 + FalI AAGNNNNNCTT 1403 1398 1435 1430 1412 1422 - FalI AAGNNNNNCTT 1435 1430 1403 1398 1424 1427 + MseI TTAA 1424 1426 . . 1427 1432 + ApoI RAATTY 1427 1431 . . 1428 1431 + TspEI AATT 1427 1431 . . 1443 1448 + AhaIII* TTTAAA 1445 1445 . . 1444 1447 + MseI TTAA 1444 1446 . . 1448 1451 + SetI ASST 1451 1447 . . 1452 1455 + CviRI* TGCA 1453 1453 . . 1459 1469 + BplI GAGNNNNNCTC 1450 1445 1482 1477 1463 1468 + TatI WGTACW 1463 1467 . . 1463 1468 + ScaI AGTACT 1465 1465 . . 1464 1467 + Csp6I GTAC 1464 1466 . . 1464 1467 + RsaI GTAC 1465 1465 . . 1459 1469 - BplI GAGNNNNNCTC 1482 1477 1450 1445 1483 1488 + SacI GAGCTC 1487 1483 . . 1483 1488 + Ecl136II GAGCTC 1485 1485 . . 1483 1488 + SduI GDGCHC 1487 1483 . . 1483 1488 + HgiJII* GRGCYC 1487 1483 . . 1483 1488 + HgiAI* GWGCWC 1487 1483 . . 1484 1487 + SetI ASST 1487 1483 . . 1484 1487 + CviJI RGCY 1485 1485 . . 1484 1487 + AluI AGCT 1485 1485 . . 1498 1503 + ApoI RAATTY 1498 1502 . . 1499 1502 + TspEI AATT 1498 1502 . . 1502 1505 + MseI TTAA 1502 1504 . . 1511 1516 + ApoI RAATTY 1511 1515 . . 1512 1515 + TspEI AATT 1511 1515 . . 1516 1520 + Hpy188I TCNGA 1518 1517 . . 1519 1523 + TfiI GAWTC 1519 1522 . . 1519 1523 + HinfI GANTC 1519 1522 . . 1531 1535 + MboII GAAGA 1543 1542 . . 1527 1532 - MmeI TCCRAC 1508 1506 . . 1535 1539 + TspDTI ATGAA 1550 1548 . . 1553 1557 + TspDTI ATGAA 1568 1566 . . 1559 1564 + ApoI RAATTY 1559 1563 . . 1560 1563 + TspEI AATT 1559 1563 . . 1569 1573 + DdeI CTNAG 1569 1572 . . 1572 1575 + CviJI RGCY 1573 1573 . . 1573 1577 + TauI GCSGC 1576 1573 . . 1573 1577 + BlsI GCNGC 1575 1574 . . 1573 1577 + BisI GCNGC 1574 1575 . . 1574 1577 + AciI CCGC 1574 1576 . . 1589 1592 + SetI ASST 1592 1588 . . 1589 1592 + CviJI RGCY 1590 1590 . . 1589 1592 + AluI AGCT 1590 1590 . . 1606 1609 - MnlI CCTC 1599 1598 . . 1612 1615 + TspEI AATT 1611 1615 . . 1615 1618 + TaqI TCGA 1615 1617 . . 1617 1627 + Hin4I GAYNNNNNVTC 1608 1603 1640 1635 1630 1635 + NdeI CATATG 1631 1633 . . 1626 1630 - HphI GGTGA 1618 1617 . . 1633 1637 + TspDTI ATGAA 1648 1646 . . 1660 1664 + TfiI GAWTC 1660 1663 . . 1660 1664 + HinfI GANTC 1660 1663 . . 1666 1670 + DdeI CTNAG 1666 1669 . . 1665 1671 - Bpu10I CCTNAGC 1666 1669 . . 1681 1685 + MboII GAAGA 1693 1692 . . 1685 1690 + TatI WGTACW 1685 1689 . . 1686 1689 + Csp6I GTAC 1686 1688 . . 1681 1686 - Ksp632I* CTCTTC 1676 1679 . . 1686 1689 + RsaI GTAC 1687 1687 . . 1697 1700 + Hin6I GCGC 1697 1699 . . 1697 1700 + GlaI GCGC 1698 1698 . . 1697 1700 + HhaI GCGC 1699 1697 . . 1705 1709 + TfiI GAWTC 1705 1708 . . 1705 1709 + HinfI GANTC 1705 1708 . . 1708 1712 + Hpy188I TCNGA 1710 1709 . . 1709 1714 + Eco57MI CTGRAG 1730 1728 . . 1709 1714 + Eco57I CTGAAG 1730 1728 . . 1711 1715 + MboII GAAGA 1723 1722 . . 1711 1716 + BbvII* GAAGAC 1718 1722 . . 1719 1722 + TspEI AATT 1718 1722 . . 1723 1726 - MnlI CCTC 1716 1715 . . 1734 1737 + TspEI AATT 1733 1737 . . 1749 1754 + ApoI RAATTY 1749 1753 . . 1750 1753 + TspEI AATT 1749 1753 . . 1765 1769 + Hpy188I TCNGA 1767 1766 . . 1775 1778 + DpnI GATC 1776 1776 . . 1775 1778 + BstKTI GATC 1777 1775 . . 1775 1778 + MboI GATC 1774 1778 . . 1768 1778 - Hin4I GAYNNNNNVTC 1791 1786 1759 1754 1799 1803 + TspDTI ATGAA 1814 1812 . . 1806 1809 + DpnI GATC 1807 1807 . . 1806 1809 + BstKTI GATC 1808 1806 . . 1806 1809 + MboI GATC 1805 1809 . . 1814 1817 + SetI ASST 1817 1813 . . 1815 1818 + MnlI CCTC 1825 1824 . . 1820 1824 + DdeI CTNAG 1820 1823 . . 1820 1824 + BspCNI CTCAG 1833 1831 . . 1820 1824 + BseMII CTCAG 1834 1832 . . 1821 1825 + Hpy188I TCNGA 1823 1822 . . 1824 1828 + TfiI GAWTC 1824 1827 . . 1824 1828 + HinfI GANTC 1824 1827 . . 1840 1844 + MboII GAAGA 1852 1851 . . 1846 1856 + BplI GAGNNNNNCTC 1837 1832 1869 1864 1852 1855 + SetI ASST 1855 1851 . . 1852 1855 + CviJI RGCY 1853 1853 . . 1852 1855 + AluI AGCT 1853 1853 . . 1846 1856 - BplI GAGNNNNNCTC 1869 1864 1837 1832 1858 1861 + MseI TTAA 1858 1860 . . 1869 1872 + TspEI AATT 1868 1872 . . 1876 1879 + SetI ASST 1879 1875 . . 1878 1881 + Csp6I GTAC 1878 1880 . . 1878 1881 + RsaI GTAC 1879 1879 . . 1888 1897 + BsaBI GATNNNNATC 1892 1892 . . 1897 1902 + MfeI CAATTG 1897 1901 . . 1898 1901 + TspEI AATT 1897 1901 . . 1906 1910 + Hpy188I TCNGA 1908 1907 . . 1922 1927 + MmeI TCCRAC 1947 1945 . . 1927 1930 + FatI CATG 1926 1930 . . 1927 1930 + NlaIII CATG 1930 1926 . . 1927 1930 + CviAII CATG 1927 1929 . . 1931 1934 + SetI ASST 1934 1930 . . 1935 1940 + ApoI RAATTY 1935 1939 . . 1936 1939 + TspEI AATT 1935 1939 . . 1953 1958 + Hpy178III* TCNNGA 1954 1956 . . 1966 1969 + MseI TTAA 1966 1968 . . 1980 1984 + FokI GGATG 1993 1997 . . 1980 1984 + BseGI GGATG 1986 1984 . . 1978 1981 - MnlI CCTC 1971 1970 . . 1982 1986 + TspDTI ATGAA 1997 1995 . . 1985 1988 + TspEI AATT 1984 1988 . . 1987 1990 + MseI TTAA 1987 1989 . . 1992 1996 + Tsp45I GTSAC 1991 1996 . . 1992 1996 + MaeIII GTNAC 1991 1996 . . 1996 2000 + DdeI CTNAG 1996 1999 . . 2002 2006 + TfiI GAWTC 2002 2005 . . 2002 2006 + HinfI GANTC 2002 2005 . . 2013 2016 + TspEI AATT 2012 2016 . . 2027 2031 + BsrI ACTGG 2032 2030 . . 2031 2035 + MboII GAAGA 2043 2042 . . 2031 2036 - Ksp632I* CTCTTC 2026 2029 . . 2054 2060 + TspRI CASTGNN 2060 2051 . . 2055 2059 + BsrI ACTGG 2060 2058 . . 2073 2078 + Hpy166II GTNNAC 2075 2075 . . 2073 2078 + HindII GTYRAC 2075 2075 . . 2086 2090 + TfiI GAWTC 2086 2089 . . 2086 2090 + HinfI GANTC 2086 2089 . . 2090 2093 + AciI CCGC 2090 2092 . . 2092 2096 + SfaNI GCATC 2101 2105 . . 2095 2099 + Hpy188I TCNGA 2097 2096 . . 2106 2109 + Hin6I GCGC 2106 2108 . . 2106 2109 + GlaI GCGC 2107 2107 . . 2106 2109 + HhaI GCGC 2108 2106 . . 2107 2110 + FnuDII* CGCG 2108 2108 . . 2112 2117 + EcoRV GATATC 2114 2114 . . 2112 2121 + BsaBI GATNNNNATC 2116 2116 . . 2117 2121 + BccI CCATC 2125 2126 . . 2120 2125 + Hpy178III* TCNNGA 2121 2123 . . 2138 2142 + TspDTI ATGAA 2153 2151 . . 2170 2174 + TfiI GAWTC 2170 2173 . . 2170 2174 + HinfI GANTC 2170 2173 . . 2191 2194 + FnuDII* CGCG 2192 2192 . . 2197 2202 + SfeI* CTRYAG 2197 2201 . . 2204 2207 + SetI ASST 2207 2203 . . 2205 2209 + HphI GGTGA 2217 2216 . . 2202 2206 - Hin4II* CCTTC 2196 2195 . . 2221 2224 + DpnI GATC 2222 2222 . . 2221 2224 + BstKTI GATC 2223 2221 . . 2221 2224 + MboI GATC 2220 2224 . . 2231 2235 + MboII GAAGA 2243 2242 . . 2231 2236 - Ksp632I* CTCTTC 2226 2229 . . 2242 2247 + BseRI GAGGAG 2257 2255 . . 2242 2245 - MnlI CCTC 2235 2234 . . 2252 2256 + TspDTI ATGAA 2267 2265 . . 2266 2271 + SspI AATATT 2268 2268 . . 2277 2280 + FatI CATG 2276 2280 . . 2277 2280 + NlaIII CATG 2280 2276 . . 2277 2280 + CviAII CATG 2277 2279 . . 2281 2285 + MboII GAAGA 2293 2292 . . 2281 2286 + BbvII* GAAGAC 2288 2292 . . 2294 2297 + MaeI CTAG 2294 2296 . . 2308 2311 + TspEI AATT 2307 2311 . . 2315 2326 + BdaI TGANNNNNNTCA 2304 2302 2338 2336 2315 2326 - BdaI TGANNNNNNTCA 2338 2336 2304 2302 2338 2341 - MnlI CCTC 2331 2330 . . 2349 2353 - BsmAI GTCTC 2343 2347 . . 2356 2360 + MboII GAAGA 2368 2367 . . 2364 2367 + MnlI CCTC 2374 2373 . . 2371 2374 + MseI TTAA 2371 2373 . . 2379 2382 - MnlI CCTC 2372 2371 . . 2395 2399 + TfiI GAWTC 2395 2398 . . 2395 2399 + HinfI GANTC 2395 2398 . . 2405 2408 + CviRI* TGCA 2406 2406 . . 2408 2411 + TspEI AATT 2407 2411 . . 2410 2413 + MseI TTAA 2410 2412 . . 2425 2428 + SetI ASST 2428 2424 . . 2425 2428 + CviJI RGCY 2426 2426 . . 2425 2428 + AluI AGCT 2426 2426 . . 2430 2435 + PsiI TTATAA 2432 2432 . . 2435 2438 + TspEI AATT 2434 2438 . . 2448 2452 + DdeI CTNAG 2448 2451 . . 2448 2452 + BspCNI CTCAG 2461 2459 . . 2448 2452 + BseMII CTCAG 2462 2460 . . 2449 2453 + Hpy188I TCNGA 2451 2450 . . 2470 2473 + DpnI GATC 2471 2471 . . 2470 2473 + BstKTI GATC 2472 2470 . . 2470 2473 + MboI GATC 2469 2473 . . 2473 2478 + SmlI CTYRAG 2473 2477 . . 2473 2478 + AflII CTTAAG 2473 2477 . . 2474 2477 + MseI TTAA 2474 2476 . . 2477 2480 + SetI ASST 2480 2476 . . 2478 2482 + HphI GGTGA 2490 2489 . . 2480 2485 + BclI TGATCA 2480 2484 . . 2481 2484 + DpnI GATC 2482 2482 . . 2481 2484 + BstKTI GATC 2483 2481 . . 2481 2484 + MboI GATC 2480 2484 . . 2493 2498 + AhaIII* TTTAAA 2495 2495 . . 2494 2497 + MseI TTAA 2494 2496 . . 2501 2504 + SetI ASST 2504 2500 . . 2515 2521 + SanDI GGGWCCC 2516 2519 . . 2515 2521 + PpuMI RGGWCCY 2516 2519 . . 2515 2521 + DraII RGGNCCY 2516 2519 . . 2515 2519 + BslFI GGGAC 2529 2533 . . 2515 2520 + NlaIV GGNNCC 2517 2517 . . 2516 2521 + NlaIV GGNNCC 2518 2518 . . 2516 2520 + BmgT120I GGNCC 2517 2518 . . 2516 2520 + AvaII GGWCC 2516 2519 . . 2516 2520 + AsuI* GGNCC 2516 2519 . . 2521 2525 + Hpy99I CGWCG 2525 2520 . . 2527 2530 + MseI TTAA 2527 2529 . . 2547 2550 + SetI ASST 2550 2546 . . 2548 2552 + HphI GGTGA 2560 2559 . . 2551 2556 + SduI GDGCHC 2555 2551 . . 2551 2556 + HgiAI* GWGCWC 2555 2551 . . 2555 2559 + Tsp4CI* ACNGT 2557 2556 . . 2574 2579 + Hpy178III* TCNNGA 2575 2577 . . 2585 2589 + TspDTI ATGAA 2600 2598 . . 2590 2593 + MaeI CTAG 2590 2592 . . 2593 2596 + DpnI GATC 2594 2594 . . 2593 2596 + BstKTI GATC 2595 2593 . . 2593 2596 + MboI GATC 2592 2596 . . 2595 2600 + Hpy178III* TCNNGA 2596 2598 . . 2610 2615 + TsoI TARCCA 2626 2624 . . 2611 2614 + CviJI RGCY 2612 2612 . . 2614 2617 + FatI CATG 2613 2617 . . 2614 2617 + NlaIII CATG 2617 2613 . . 2614 2617 + CviAII CATG 2614 2616 . . 2626 2629 + SetI ASST 2629 2625 . . 2631 2636 + Hpy178III* TCNNGA 2632 2634 . . 2631 2636 + BspHI TCATGA 2631 2635 . . 2632 2641 + MslI CAYNNNNRTG 2636 2636 . . 2632 2635 + FatI CATG 2631 2635 . . 2632 2635 + NlaIII CATG 2635 2631 . . 2632 2635 + CviAII CATG 2632 2634 . . 2639 2643 + TspDTI ATGAA 2654 2652 . . 2646 2650 + FauI CCCGC 2654 2656 . . 2647 2650 + AciI CCGC 2647 2649 . . 2650 2654 + DdeI CTNAG 2650 2653 . . 2654 2658 + TfiI GAWTC 2654 2657 . . 2654 2658 + HinfI GANTC 2654 2657 . . 2659 2662 + TspEI AATT 2658 2662 . . 2661 2666 + AhaIII* TTTAAA 2663 2663 . . 2662 2665 + MseI TTAA 2662 2664 . . 2669 2673 + MboII GAAGA 2681 2680 . . 2673 2676 + SetI ASST 2676 2672 . . 2673 2679 + PpuMI RGGWCCY 2674 2677 . . 2673 2679 + DraII RGGNCCY 2674 2677 . . 2669 2674 - Ksp632I* CTCTTC 2664 2667 . . 2674 2679 + NlaIV GGNNCC 2676 2676 . . 2674 2678 + BmgT120I GGNCC 2675 2676 . . 2674 2678 + AvaII GGWCC 2674 2677 . . 2674 2678 + AsuI* GGNCC 2674 2677 . . 2672 2675 - MnlI CCTC 2665 2664 . . 2675 2679 - BslFI GGGAC 2660 2664 . . 2683 2688 + AsuII TTCGAA 2684 2686 . . 2684 2687 + TaqI TCGA 2684 2686 . . 2690 2694 + TauI GCSGC 2693 2690 . . 2690 2700 + MwoI GCNNNNNNNGC 2696 2693 . . 2690 2694 + BlsI GCNGC 2692 2691 . . 2690 2694 + BisI GCNGC 2691 2692 . . 2691 2694 + AciI CCGC 2691 2693 . . 2694 2699 + SmlI CTYRAG 2694 2698 . . 2694 2699 + AflII CTTAAG 2694 2698 . . 2695 2698 + MseI TTAA 2695 2697 . . 2697 2702 + HindIII AAGCTT 2697 2701 . . 2698 2701 + SetI ASST 2701 2697 . . 2698 2701 + CviJI RGCY 2699 2699 . . 2698 2701 + AluI AGCT 2699 2699 . . 2707 2711 + Tsp4CI* ACNGT 2709 2708 . . 2724 2729 + Cac8I GCNNGC 2726 2726 . . 2738 2742 + FokI GGATG 2751 2755 . . 2738 2742 + BseGI GGATG 2744 2742 . . 2736 2739 - MnlI CCTC 2729 2728 . . 2740 2744 + TspDTI ATGAA 2755 2753 . . 2745 2755 + FalI AAGNNNNNCTT 2736 2731 2768 2763 2745 2755 - FalI AAGNNNNNCTT 2768 2763 2736 2731 2765 2768 + DpnI GATC 2766 2766 . . 2765 2768 + BstKTI GATC 2767 2765 . . 2765 2768 + MboI GATC 2764 2768 . . 2767 2771 + Hpy188I TCNGA 2769 2768 . . 2791 2795 + DdeI CTNAG 2791 2794 . . 2802 2805 + TspEI AATT 2801 2805 . . 2813 2817 + BlsI GCNGC 2815 2814 . . 2813 2817 + BisI GCNGC 2814 2815 . . 2813 2817 + BbvI GCAGC 2825 2829 . . 2813 2817 + TseI GCWGC 2813 2816 . . 2814 2819 + NspBII* CMGCKG 2816 2816 . . 2816 2821 + Cac8I GCNNGC 2818 2818 . . 2816 2819 - AciI CCGC 2816 2818 . . 2816 2820 - FauI CCCGC 2809 2811 . . #--------------------------------------- # # # AciI: # [CCGC] # 19 44 125 265 516 557 # 1309 # # AcyI: # [GRCGYC] # 623 2212 # # AflII: # [CTTAAG] # 141 221 2473 # # AhaIII*: # [TTTAAA] # 168 172 660 785 1050 # # AluI: # [AGCT] # 19 32 75 84 84 84 # 105 136 150 188 263 273 # 379 390 573 # # ApoI: # [RAATTY] # 13 46 48 61 71 98 # 141 144 174 186 189 190 # 263 311 900 # # AsuI*: # [GGNCC] # 151 158 161 2365 # # AsuII: # [TTCGAA] # 151 2684 # # AvaII: # [GGWCC] # 158 161 2516 # # BbvI: # [GCAGC] # 10 2825 # # BbvII*: # [GAAGAC] # 547 570 684 1034 # # BccI: # [CCATC] # 710 939 1186 # # Bce83I*: # [CTTGAG] # 1200 1635 # # BciVI: # [GTATCC] # 858 1977 # # BclI: # [TGATCA] # 355 660 1820 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 497 2304 # # BfiI: # [ACTGGG] # 1200 1635 # # BglII: # [AGATCT] # 793 2042 # # BisI: # [GCNGC] # 21 123 1117 1574 # # BlsI: # [GCNGC] # 20 123 1117 1575 # # BmgT120I: # [GGNCC] # 152 158 160 2365 # # BplI: # [GAGNNNNNCTC] # 0 0 0 0 32 32 # 355 966 1450 # # Bpu10I: # [CCTNAGC] # 1169 1666 # # BsaBI: # [GATNNNNATC] # 224 719 1892 # # BseGI: # [GGATG] # 91 134 492 676 684 758 # # BseMII: # [CTCAG] # 126 165 361 373 525 628 # 657 # # BseRI: # [GAGGAG] # 578 2257 # # BsiYI*: # [CCNNNNNNNGG] # 280 2555 # # BslFI: # [GGGAC] # 131 175 2529 # # BsmAI: # [GTCTC] # 350 492 843 1150 # # BspCNI: # [CTCAG] # 126 163 360 374 527 628 # 657 # # BspHI: # [TCATGA] # 204 2631 # # BsrDI: # [GCAATG] # 291 564 1980 # # BsrI: # [ACTGG] # 28 775 826 1206 # # BstKTI: # [GATC] # 11 31 68 112 133 172 # 249 415 463 518 663 # # Cac8I: # [GCNNGC] # 17 92 2726 # # Csp6I: # [GTAC] # 192 222 689 775 957 # # CviAII: # [CATG] # 18 42 203 337 350 466 # 1419 # # CviJI: # [RGCY] # 5 5 13 17 17 19 # 32 37 47 55 70 79 # 84 87 88 91 95 120 # 136 150 186 263 267 299 # 573 # # CviRI*: # [TGCA] # 179 195 405 429 674 953 # # DdeI: # [CTNAG] # 44 97 98 113 126 141 # 142 154 176 202 234 406 # 450 452 # # DpnI: # [GATC] # 11 31 69 112 133 172 # 249 415 463 518 662 # # DraII: # [RGGNCCY] # 151 158 161 2365 # # Eam1105I: # [GACNNNNNGTC] # 9 2826 # # EciI: # [GGCGGA] # 254 2581 # # Ecl136II: # [GAGCTC] # 1350 1485 # # Eco47III: # [AGCGCT] # 605 2230 # # Eco57I: # [CTGAAG] # 1105 1730 # # Eco57MI: # [CTGRAG] # 1105 1730 # # EcoRI: # [GAATTC] # 235 642 1958 # # EcoRV: # [GATATC] # 721 851 1263 # # EcoT22I: # [ATGCAT] # 676 2159 # # EspI*: # [GCTNAGC] # 1163 1672 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 67 1301 1403 # # FatI: # [CATG] # 18 41 204 337 350 466 # 1419 # # FauI: # [CCCGC] # 26 155 2654 # # FnuDII*: # [CGCG] # 84 643 2108 # # FokI: # [GGATG] # 84 134 492 683 684 758 # # GlaI: # [GCGC] # 409 605 728 1093 # # HaeII: # [RGCGCY] # 607 2228 # # HaeIII: # [GGCC] # 152 2683 # # HgaI: # [GACGC] # 631 2204 # # HgiAI*: # [GWGCWC] # 280 1068 1487 # # HgiJII*: # [GRGCYC] # 271 1216 1348 # # HhaI: # [GCGC] # 409 606 727 1093 # # Hin4I: # [GAYNNNNNVTC] # 0 0 27 32 32 32 # 119 280 1044 1269 # # Hin4II*: # [CCTTC] # 142 639 990 1064 # # Hin6I: # [GCGC] # 409 604 729 1093 # # HindII: # [GTYRAC] # 291 760 1784 # # HindIII: # [AAGCTT] # 138 218 2479 # # HinfI: # [GANTC] # 30 45 84 84 114 119 # 141 174 178 181 196 225 # 225 259 780 # # HphI: # [GGTGA] # 70 148 273 275 599 1470 # # Hpy166II: # [GTNNAC] # 291 397 498 760 889 # # Hpy178III*: # [TCNNGA] # 21 21 36 152 167 203 # 214 244 251 384 454 688 # # Hpy188I: # [TCNGA] # 56 57 66 85 189 192 # 318 354 459 480 579 # # Hpy99I: # [CGWCG] # 310 2525 # # Ksp632I*: # [CTCTTC] # 171 200 256 350 438 582 # 838 # # MaeI: # [CTAG] # 66 158 243 245 269 296 # 324 342 892 # # MaeII: # [ACGT] # 89 684 2062 # # MaeIII: # [GTNAC] # 11 758 844 1222 # # MboI: # [GATC] # 11 31 71 112 133 172 # 249 415 463 518 660 # # MboII: # [GAAGA] # 3 3 13 17 30 30 # 48 50 60 69 75 77 # 91 123 129 134 137 150 # 154 154 166 181 191 200 # 237 313 # # MfeI: # [CAATTG] # 191 723 938 983 # # MlyI: # [GAGTC] # 189 190 1005 1451 # # MmeI: # [TCCRAC] # 439 654 854 888 # # MnlI: # [CCTC] # 2 41 64 95 96 106 # 109 117 146 183 199 264 # 291 327 795 # # MseI: # [TTAA] # 12 20 20 21 30 33 # 33 34 36 38 39 58 # 64 72 93 96 96 108 # 126 135 138 140 158 234 # 261 356 384 # # MslI: # [CAYNNNNRTG] # 199 2636 # # MwoI: # [GCNNNNNNNGC] # 139 2696 # # NdeI: # [CATATG] # 1204 1631 # # NlaIII: # [CATG] # 18 45 200 337 350 466 # 1419 # # NlaIV: # [GGNNCC] # 1 119 158 159 2398 # # NmeAIII: # [GCCGAG] # 1113 1722 # # NspBII*: # [CMGCKG] # 19 2816 # # PleI: # [GAGTC] # 188 190 1005 1452 # # PpuMI: # [RGGWCCY] # 158 161 2516 # # PshAI: # [GACNNNNGTC] # 296 2539 # # PsiI: # [TTATAA] # 403 2432 # # RsaI: # [GTAC] # 192 222 689 776 956 # # SacI: # [GAGCTC] # 1348 1487 # # SanDI: # [GGGWCCC] # 319 2516 # # SapI: # [GCTCTTC] # 838 1997 # # ScaI: # [AGTACT] # 1370 1465 # # SduI: # [GDGCHC] # 271 280 1068 1216 # # SecI*: # [CCNNGG] # 274 2561 # # SetI: # [ASST] # 9 19 24 24 25 26 # 34 36 38 39 46 47 # 49 52 55 67 75 79 # 84 84 85 89 103 105 # 114 134 221 225 234 273 # 340 # # SfaNI: # [GCATC] # 498 661 734 942 # # SfeI*: # [CTRYAG] # 56 638 2141 # # SmlI: # [CTYRAG] # 141 221 1215 1258 # # SspI: # [AATATT] # 567 2268 # # StyI: # [CCWWGG] # 274 2561 # # TaiI: # [ACGT] # 89 687 2059 # # TaqI: # [TCGA] # 151 230 1069 1385 # # TatI: # [WGTACW] # 222 1150 1463 # # TauI: # [GCSGC] # 142 1117 1576 # # TfiI: # [GAWTC] # 45 84 84 114 119 141 # 178 181 225 259 429 976 # # TseI: # [GCWGC] # 22 2813 # # TsoI: # [TARCCA] # 209 2626 # # Tsp45I: # [GTSAC] # 11 758 844 1222 # # Tsp4CI*: # [ACNGT] # 85 126 137 152 163 252 # 329 406 1185 # # TspDTI: # [ATGAA] # 13 17 18 21 21 21 # 54 54 63 63 80 80 # 99 101 103 114 138 156 # 166 183 240 297 333 400 # # TspEI: # [AATT] # 7 8 13 15 16 21 # 21 22 22 27 28 29 # 32 32 33 34 35 36 # 38 39 42 42 46 48 # 49 52 54 56 58 61 # 67 70 71 79 83 84 # 100 107 112 119 120 143 # 145 224 295 # # TspGWI: # [ACGGA] # 1317 1518 # # TspRI: # [CASTGNN] # 683 775 1377 # # VspI: # [ATTAAT] # 485 671 1679 # # XhoII: # [RGATCY] # 793 2042 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2835 # Reported_sequences: 1 # Reported_hitcount: 571 #---------------------------------------