Restriction Fragments for BFR2/YDR299W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

BFR2/YDR299W on chromosome IV from coordinates 1059627 to 1061231.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28585.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 16 + MaeI CTAG 13 15 . . 18 22 + BinI* GGATC 26 27 . . 19 22 + DpnI GATC 20 20 . . 19 22 + BstKTI GATC 21 19 . . 19 22 + MboI GATC 18 22 . . 16 19 - AciI CCGC 16 18 . . 23 28 + ApoI RAATTY 23 27 . . 24 27 + TspEI AATT 23 27 . . 28 32 + Hpy188I TCNGA 30 29 . . 31 36 + EcoRV GATATC 33 33 . . 41 44 + MseI TTAA 41 43 . . 45 50 + Cfr10I RCCGGY 45 49 . . 45 50 + BetI* WCCGGW 45 49 . . 45 50 + AgeI ACCGGT 45 49 . . 46 49 + HpaII CCGG 46 48 . . 62 65 + TaqI TCGA 62 64 . . 70 74 + MboII GAAGA 82 81 . . 76 79 - MnlI CCTC 69 68 . . 83 88 + EcoT22I ATGCAT 87 83 . . 84 87 + CviRI* TGCA 85 85 . . 85 89 + SfaNI GCATC 94 98 . . 104 108 + TspDTI ATGAA 119 117 . . 127 130 + MseI TTAA 127 129 . . 154 158 + MboII GAAGA 166 165 . . 165 169 - Hin4II* CCTTC 159 158 . . 169 172 + Hin6I GCGC 169 171 . . 169 172 + GlaI GCGC 170 170 . . 169 172 + HhaI GCGC 171 169 . . 182 185 + SetI ASST 185 181 . . 181 184 - MnlI CCTC 174 173 . . 194 198 + DdeI CTNAG 194 197 . . 199 202 + MseI TTAA 199 201 . . 213 216 + SetI ASST 216 212 . . 229 232 + DpnI GATC 230 230 . . 229 232 + BstKTI GATC 231 229 . . 229 232 + MboI GATC 228 232 . . 229 233 - BinI* GGATC 223 224 . . 243 246 + SetI ASST 246 242 . . 248 251 + MseI TTAA 248 250 . . 252 255 + SetI ASST 255 251 . . 256 261 + Hpy178III* TCNNGA 257 259 . . 272 276 + TspDTI ATGAA 287 285 . . 274 278 + MboII GAAGA 286 285 . . 283 287 + Hpy188I TCNGA 285 284 . . 292 295 - MnlI CCTC 285 284 . . 298 302 + MboII GAAGA 310 309 . . 301 305 + MboII GAAGA 313 312 . . 304 308 + MboII GAAGA 316 315 . . 307 311 + MboII GAAGA 319 318 . . 310 314 + MboII GAAGA 322 321 . . 313 317 + MboII GAAGA 325 324 . . 313 318 - Ksp632I* CTCTTC 308 311 . . 316 319 - MnlI CCTC 309 308 . . 319 323 + MboII GAAGA 331 330 . . 322 326 + MboII GAAGA 334 333 . . 331 334 - MnlI CCTC 324 323 . . 334 338 + MboII GAAGA 346 345 . . 335 345 + FalI AAGNNNNNCTT 326 321 358 353 337 341 - SfaNI GCATC 327 331 . . 335 345 - FalI AAGNNNNNCTT 358 353 326 321 345 349 - TspDTI ATGAA 335 333 . . 350 355 + Hpy178III* TCNNGA 351 353 . . 356 364 + AlwNI CAGNNNCTG 361 358 . . 358 362 + TfiI GAWTC 358 361 . . 358 362 + HinfI GANTC 358 361 . . 361 365 + Hpy188I TCNGA 363 362 . . 362 367 + Eco57MI CTGRAG 383 381 . . 362 367 + Eco57I CTGAAG 383 381 . . 364 368 + MboII GAAGA 376 375 . . 368 372 + TspDTI ATGAA 383 381 . . 370 374 + MboII GAAGA 382 381 . . 386 390 + TspDTI ATGAA 401 399 . . 388 392 + MboII GAAGA 400 399 . . 391 395 + MboII GAAGA 403 402 . . 394 398 + TfiI GAWTC 394 397 . . 394 398 + HinfI GANTC 394 397 . . 397 401 + Hpy188I TCNGA 399 398 . . 400 404 + HgaI GACGC 409 414 . . 402 405 + FnuDII* CGCG 403 403 . . 403 406 - AciI CCGC 403 405 . . 407 411 + BceAI ACGGC 423 425 . . 412 416 + HphI GGTGA 424 423 . . 410 413 - AciI CCGC 410 412 . . 418 422 + TspGWI ACGGA 433 431 . . 421 426 + BseRI GAGGAG 436 434 . . 421 424 - MnlI CCTC 414 413 . . 426 429 + CviJI RGCY 427 427 . . 424 427 - MnlI CCTC 417 416 . . 441 446 + NspI RCATGY 445 441 . . 441 446 + Cac8I GCNNGC 443 443 . . 441 446 + SphI GCATGC 445 441 . . 442 445 + FatI CATG 441 445 . . 442 445 + NlaIII CATG 445 441 . . 442 445 + CviAII CATG 442 444 . . 444 447 + CviRI* TGCA 445 445 . . 451 454 + TaqI TCGA 451 453 . . 459 462 + TspEI AATT 458 462 . . 469 473 - BsmAI GTCTC 463 467 . . 480 483 + SetI ASST 483 479 . . 480 483 + CviJI RGCY 481 481 . . 480 483 + AluI AGCT 481 481 . . 485 488 + MseI TTAA 485 487 . . 492 496 + Tsp4CI* ACNGT 494 493 . . 495 499 + BsmAI GTCTC 500 504 . . 510 520 + FalI AAGNNNNNCTT 501 496 533 528 514 518 - SfaNI GCATC 504 508 . . 519 524 + AsuII TTCGAA 520 522 . . 520 523 + TaqI TCGA 520 522 . . 510 520 - FalI AAGNNNNNCTT 533 528 501 496 522 526 - Hin4II* CCTTC 516 515 . . 551 554 + TspEI AATT 550 554 . . 594 597 + SetI ASST 597 593 . . 594 597 + CviJI RGCY 595 595 . . 594 597 + AluI AGCT 595 595 . . 595 605 + MwoI GCNNNNNNNGC 601 598 . . 600 603 + TspEI AATT 599 603 . . 603 606 + CviRI* TGCA 604 604 . . 604 608 + BlsI GCNGC 606 605 . . 604 608 + BisI GCNGC 605 606 . . 604 608 + BbvI GCAGC 616 620 . . 604 608 + TseI GCWGC 604 607 . . 614 617 + SetI ASST 617 613 . . 614 617 + CviJI RGCY 615 615 . . 614 617 + AluI AGCT 615 615 . . 622 625 + MseI TTAA 622 624 . . 626 631 + SfeI* CTRYAG 626 630 . . 631 635 + PleI GAGTC 639 640 . . 631 635 + MlyI GAGTC 640 640 . . 631 635 + HinfI GANTC 631 634 . . 635 639 + BssKI CCNGG 634 639 . . 635 639 + BseBI CCWGG 636 637 . . 635 640 + SecI* CCNNGG 635 639 . . 635 639 + ScrFI CCNGG 636 637 . . 635 639 + EcoRII CCWGG 634 639 . . 640 644 + MboII GAAGA 652 651 . . 645 648 + CviJI RGCY 646 646 . . 640 645 - Ksp632I* CTCTTC 635 638 . . 643 646 - MnlI CCTC 636 635 . . 654 658 + FokI GGATG 667 671 . . 654 658 + BseGI GGATG 660 658 . . 658 662 + TfiI GAWTC 658 661 . . 658 662 + HinfI GANTC 658 661 . . 661 665 + Hpy188I TCNGA 663 662 . . 664 667 - MnlI CCTC 657 656 . . 682 687 + Eco57MI CTGRAG 703 701 . . 682 687 + Eco57I CTGAAG 703 701 . . 684 688 - Hin4II* CCTTC 678 677 . . 699 703 + Tsp4CI* ACNGT 701 700 . . 709 712 + TspEI AATT 708 712 . . 725 736 + BdaI TGANNNNNNTCA 714 712 748 746 729 734 + ApoI RAATTY 729 733 . . 730 733 + TspEI AATT 729 733 . . 734 738 + Hpy188I TCNGA 736 735 . . 725 736 - BdaI TGANNNNNNTCA 748 746 714 712 742 747 + ApoI RAATTY 742 746 . . 743 746 + TspEI AATT 742 746 . . 746 751 + MmeI TCCRAC 771 769 . . 757 760 + DpnI GATC 758 758 . . 757 760 + BstKTI GATC 759 757 . . 757 760 + MboI GATC 756 760 . . 773 777 + TspDTI ATGAA 788 786 . . 775 779 + MboII GAAGA 787 786 . . 779 782 + SetI ASST 782 778 . . 775 780 - Ksp632I* CTCTTC 770 773 . . 778 781 - MnlI CCTC 771 770 . . 794 797 + TspEI AATT 793 797 . . 808 813 + XhoII RGATCY 808 812 . . 808 813 + BglII AGATCT 808 812 . . 809 812 + DpnI GATC 810 810 . . 809 812 + BstKTI GATC 811 809 . . 809 812 + MboI GATC 808 812 . . 821 824 + SetI ASST 824 820 . . 821 824 + CviJI RGCY 822 822 . . 821 824 + AluI AGCT 822 822 . . 841 844 + SetI ASST 844 840 . . 841 844 + CviJI RGCY 842 842 . . 841 844 + AluI AGCT 842 842 . . 843 847 + DdeI CTNAG 843 846 . . 847 851 + HinfI GANTC 847 850 . . 849 853 + DdeI CTNAG 849 852 . . 849 853 + BspCNI CTCAG 862 860 . . 849 853 + BseMII CTCAG 863 861 . . 850 854 + Hpy188I TCNGA 852 851 . . 847 851 - PleI GAGTC 841 842 . . 847 851 - MlyI GAGTC 841 841 . . 863 868 + TatI WGTACW 863 867 . . 864 867 + Csp6I GTAC 864 866 . . 864 867 + RsaI GTAC 865 865 . . 874 878 - BceAI ACGGC 859 861 . . 880 883 + MseI TTAA 880 882 . . 891 896 + Hpy166II GTNNAC 893 893 . . 891 896 + AccI GTMKAC 892 894 . . 904 908 - MboII GAAGA 896 895 . . 909 912 + CviRI* TGCA 910 910 . . 910 914 + SfaNI GCATC 919 923 . . 915 918 + SetI ASST 918 914 . . 917 921 + MaeIII GTNAC 916 921 . . 922 926 + BlsI GCNGC 924 923 . . 922 926 + BisI GCNGC 923 924 . . 922 926 + TseI GCWGC 922 925 . . 922 926 - BbvI GCAGC 909 913 . . 940 945 + ApoI RAATTY 940 944 . . 941 944 + TspEI AATT 940 944 . . 948 951 + SetI ASST 951 947 . . 948 951 + CviJI RGCY 949 949 . . 948 951 + AluI AGCT 949 949 . . 960 963 + SetI ASST 963 959 . . 960 965 + BspMI ACCTGC 969 973 . . 962 967 + PstI CTGCAG 966 962 . . 962 967 + SfeI* CTRYAG 962 966 . . 963 966 + CviRI* TGCA 964 964 . . 969 974 + TatI WGTACW 969 973 . . 969 974 + Bsp1407I TGTACA 969 973 . . 970 973 + Csp6I GTAC 970 972 . . 970 973 + RsaI GTAC 971 971 . . 977 980 + TaqI TCGA 977 979 . . 986 989 + TspEI AATT 985 989 . . 991 995 + Hpy188I TCNGA 993 992 . . 999 1003 - BslFI GGGAC 984 988 . . 1009 1013 + TspDTI ATGAA 1024 1022 . . 1064 1068 + Tsp4CI* ACNGT 1066 1065 . . 1076 1081 + Cac8I GCNNGC 1078 1078 . . 1079 1082 + CviJI RGCY 1080 1080 . . 1088 1091 + TspEI AATT 1087 1091 . . 1131 1136 + EcoRI GAATTC 1131 1135 . . 1131 1136 + ApoI RAATTY 1131 1135 . . 1132 1135 + TspEI AATT 1131 1135 . . 1135 1139 + Hpy188I TCNGA 1137 1136 . . 1137 1141 + FokI GGATG 1150 1154 . . 1137 1141 + BseGI GGATG 1143 1141 . . 1145 1148 + CviJI RGCY 1146 1146 . . 1141 1145 - BccI CCATC 1135 1136 . . 1150 1155 + EcoRV GATATC 1152 1152 . . 1154 1159 + Hpy178III* TCNNGA 1155 1157 . . 1175 1179 + MboII GAAGA 1187 1186 . . 1214 1219 + NdeI CATATG 1215 1217 . . 1231 1235 + MboII GAAGA 1243 1242 . . 1241 1245 + Tsp4CI* ACNGT 1243 1242 . . 1261 1264 + MseI TTAA 1261 1263 . . 1263 1266 + TspEI AATT 1262 1266 . . 1279 1284 + MmeI TCCRAC 1304 1302 . . 1291 1294 + TspEI AATT 1290 1294 . . 1294 1298 + Hpy188I TCNGA 1296 1295 . . 1301 1306 + BsgI GTGCAG 1322 1320 . . 1302 1305 + CviRI* TGCA 1303 1303 . . 1303 1307 + BlsI GCNGC 1305 1304 . . 1303 1307 + BisI GCNGC 1304 1305 . . 1303 1307 + BbvI GCAGC 1315 1319 . . 1303 1307 + TseI GCWGC 1303 1306 . . 1308 1311 + TspEI AATT 1307 1311 . . 1318 1321 + SetI ASST 1321 1317 . . 1319 1322 + MnlI CCTC 1329 1328 . . 1316 1320 - HphI GGTGA 1308 1307 . . 1335 1340 + AsuII TTCGAA 1336 1338 . . 1336 1339 + TaqI TCGA 1336 1338 . . 1346 1349 + SetI ASST 1349 1345 . . 1346 1349 + CviJI RGCY 1347 1347 . . 1346 1349 + AluI AGCT 1347 1347 . . 1353 1357 + MboII GAAGA 1365 1364 . . 1360 1365 + ClaI ATCGAT 1361 1363 . . 1361 1364 + TaqI TCGA 1361 1363 . . 1367 1371 + DdeI CTNAG 1367 1370 . . 1371 1374 + CviJI RGCY 1372 1372 . . 1376 1381 + StyI CCWWGG 1376 1380 . . 1376 1381 + SecI* CCNNGG 1376 1380 . . 1376 1386 + BsiYI* CCNNNNNNNGG 1382 1379 . . 1388 1391 + TspEI AATT 1387 1391 . . 1398 1402 + DdeI CTNAG 1398 1401 . . 1398 1402 + BspCNI CTCAG 1411 1409 . . 1398 1402 + BseMII CTCAG 1412 1410 . . 1404 1409 + Hpy178III* TCNNGA 1405 1407 . . 1407 1412 + XhoII RGATCY 1407 1411 . . 1408 1411 + DpnI GATC 1409 1409 . . 1408 1411 + BstKTI GATC 1410 1408 . . 1408 1411 + MboI GATC 1407 1411 . . 1412 1417 + MfeI CAATTG 1412 1416 . . 1408 1412 - BinI* GGATC 1402 1403 . . 1413 1416 + TspEI AATT 1412 1416 . . 1420 1423 + TspEI AATT 1419 1423 . . 1424 1428 + TspDTI ATGAA 1439 1437 . . 1428 1431 + CviJI RGCY 1429 1429 . . 1433 1437 + BccI CCATC 1441 1442 . . 1441 1446 + Hpy178III* TCNNGA 1442 1444 . . 1441 1446 + BetI* WCCGGW 1441 1445 . . 1441 1446 + BspMII* TCCGGA 1441 1445 . . 1442 1445 + HpaII CCGG 1442 1444 . . 1439 1443 - FokI GGATG 1425 1429 . . 1439 1443 - BseGI GGATG 1438 1436 . . 1444 1449 - BciVI GTATCC 1438 1437 . . 1456 1460 + Hpy188I TCNGA 1458 1457 . . 1468 1473 + ClaI ATCGAT 1469 1471 . . 1469 1472 + TaqI TCGA 1469 1471 . . 1472 1476 + TspDTI ATGAA 1487 1485 . . 1474 1479 + EcoRI GAATTC 1474 1478 . . 1474 1479 + ApoI RAATTY 1474 1478 . . 1475 1478 + TspEI AATT 1474 1478 . . 1483 1486 - AciI CCGC 1483 1485 . . 1483 1487 - FauI CCCGC 1476 1478 . . 1494 1497 + SetI ASST 1497 1493 . . 1496 1501 + Hpy166II GTNNAC 1498 1498 . . 1496 1501 + HindII GTYRAC 1498 1498 . . 1504 1509 + Hpy166II GTNNAC 1506 1506 . . 1511 1514 + MseI TTAA 1511 1513 . . 1514 1518 + TspDTI ATGAA 1529 1527 . . 1524 1528 + FokI GGATG 1537 1541 . . 1524 1528 + BseGI GGATG 1530 1528 . . 1522 1525 - MnlI CCTC 1515 1514 . . 1528 1531 - MnlI CCTC 1521 1520 . . 1536 1541 + NspI RCATGY 1540 1536 . . 1537 1540 + FatI CATG 1536 1540 . . 1537 1540 + NlaIII CATG 1540 1536 . . 1537 1540 + CviAII CATG 1537 1539 . . 1558 1562 + MboII GAAGA 1570 1569 . . 1562 1565 + TspEI AATT 1561 1565 . . 1569 1572 + CviJI RGCY 1570 1570 . . 1567 1570 - MnlI CCTC 1560 1559 . . 1574 1577 + MseI TTAA 1574 1576 . . 1585 1590 + EcoRV GATATC 1587 1587 . . 1590 1601 + BstXI CCANNNNNNTGG 1597 1593 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 713 892 # # AciI: # [CCGC] # 7 16 122 387 1073 # # AgeI: # [ACCGGT] # 45 1560 # # AluI: # [AGCT] # 20 20 107 114 207 258 # 398 481 # # AlwNI: # [CAGNNNCTG] # 361 1244 # # ApoI: # [RAATTY] # 13 23 131 191 198 343 # 706 # # AsuII: # [TTCGAA] # 269 520 816 # # BbvI: # [GCAGC] # 290 293 406 616 # # BccI: # [CCATC] # 164 306 1135 # # BceAI: # [ACGGC] # 423 436 746 # # BciVI: # [GTATCC] # 167 1438 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 714 857 # # BetI*: # [WCCGGW] # 45 164 1396 # # BglII: # [AGATCT] # 797 808 # # BinI*: # [GGATC] # 26 197 203 1179 # # BisI: # [GCNGC] # 301 318 381 605 # # BlsI: # [GCNGC] # 300 318 381 606 # # BseBI: # [CCWGG] # 636 969 # # BseGI: # [GGATG] # 75 92 295 483 660 # # BseMII: # [CTCAG] # 193 549 863 # # BseRI: # [GAGGAG] # 436 1169 # # BsgI: # [GTGCAG] # 283 1322 # # BsiYI*: # [CCNNNNNNNGG] # 223 1382 # # BslFI: # [GGGAC] # 621 984 # # BsmAI: # [GTCTC] # 37 463 1105 # # Bsp1407I: # [TGTACA] # 636 969 # # BspCNI: # [CTCAG] # 194 549 862 # # BspMI: # [ACCTGC] # 636 969 # # BspMII*: # [TCCGGA] # 164 1441 # # BssKI: # [CCNGG] # 634 971 # # BstKTI: # [GATC] # 21 52 195 210 528 599 # # BstXI: # [CCANNNNNNTGG] # 8 1597 # # Cac8I: # [GCNNGC] # 443 527 635 # # Cfr10I: # [RCCGGY] # 45 1560 # # ClaI: # [ATCGAT] # 108 136 1361 # # Csp6I: # [GTAC] # 106 635 864 # # CviAII: # [CATG] # 68 442 1095 # # CviJI: # [RGCY] # 20 20 25 31 35 54 # 57 66 107 114 131 141 # 176 201 427 # # CviRI*: # [TGCA] # 54 85 159 302 306 339 # 360 # # DdeI: # [CTNAG] # 6 31 194 207 518 649 # # DpnI: # [GATC] # 20 52 196 210 528 599 # # Eco57I: # [CTGAAG] # 320 383 902 # # Eco57MI: # [CTGRAG] # 320 383 902 # # EcoRI: # [GAATTC] # 131 343 1131 # # EcoRII: # [CCWGG] # 634 971 # # EcoRV: # [GATATC] # 18 33 435 1119 # # EcoT22I: # [ATGCAT] # 87 1518 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 143 326 1072 # # FatI: # [CATG] # 69 441 1095 # # FauI: # [CCCGC] # 129 1476 # # FnuDII*: # [CGCG] # 403 1202 # # FokI: # [GGATG] # 68 112 275 483 667 # # GlaI: # [GCGC] # 170 1435 # # HgaI: # [GACGC] # 409 1196 # # HhaI: # [GCGC] # 171 1434 # # Hin4II*: # [CCTTC] # 159 162 357 927 # # Hin6I: # [GCGC] # 169 1436 # # HindII: # [GTYRAC] # 107 1498 # # HinfI: # [GANTC] # 27 36 189 237 358 758 # # HpaII: # [CCGG] # 46 163 1396 # # HphI: # [GGTGA] # 297 424 884 # # Hpy166II: # [GTNNAC] # 8 99 605 893 # # Hpy178III*: # [TCNNGA] # 37 94 163 250 257 804 # # Hpy188I: # [TCNGA] # 30 36 73 78 116 141 # 144 147 159 162 255 264 # # Ksp632I*: # [CTCTTC] # 135 308 327 835 # # MaeI: # [CTAG] # 13 1592 # # MaeIII: # [GTNAC] # 689 916 # # MboI: # [GATC] # 18 52 198 210 528 599 # # MboII: # [GAAGA] # 3 3 3 3 3 3 # 3 6 6 12 18 24 # 30 35 56 82 84 109 # 120 122 135 205 249 291 # # MfeI: # [CAATTG] # 193 1412 # # MlyI: # [GAGTC] # 201 640 764 # # MmeI: # [TCCRAC] # 301 533 771 # # MnlI: # [CCTC] # 3 6 15 21 24 39 # 45 69 90 105 111 114 # 186 219 558 # # MseI: # [TTAA] # 31 41 49 63 72 86 # 137 237 250 258 381 # # MwoI: # [GCNNNNNNNGC] # 601 1004 # # NdeI: # [CATATG] # 390 1215 # # NlaIII: # [CATG] # 65 445 1095 # # NspI: # [RCATGY] # 65 445 1095 # # PleI: # [GAGTC] # 202 639 764 # # PstI: # [CTGCAG] # 639 966 # # RsaI: # [GTAC] # 106 634 865 # # ScrFI: # [CCNGG] # 636 969 # # SecI*: # [CCNNGG] # 229 635 741 # # SetI: # [ASST] # 9 12 20 20 28 30 # 31 33 42 74 108 114 # 148 165 185 228 358 # # SfaNI: # [GCATC] # 94 177 233 415 686 # # SfeI*: # [CTRYAG] # 336 626 643 # # SphI: # [GCATGC] # 445 1160 # # StyI: # [CCWWGG] # 229 1376 # # TaqI: # [TCGA] # 25 62 69 108 136 359 # 389 457 # # TatI: # [WGTACW] # 106 636 863 # # TfiI: # [GAWTC] # 36 264 358 947 # # TseI: # [GCWGC] # 302 318 381 604 # # Tsp4CI*: # [ACNGT] # 177 207 362 365 494 # # TspDTI: # [ATGAA] # 18 42 48 48 48 76 # 119 168 236 387 415 # # TspEI: # [AATT] # 7 13 17 21 23 25 # 28 44 44 45 49 51 # 55 80 87 92 102 109 # 131 147 435 # # TspGWI: # [ACGGA] # 433 1172 # # XhoII: # [RGATCY] # 198 599 808 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1605 # Reported_sequences: 1 # Reported_hitcount: 319 #---------------------------------------