Restriction Fragments for DPL1/YDR294C



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The currently selected gene/sequence is :

DPL1/YDR294C on chromosome IV from coordinates 1052228 to 1050459.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.5362.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 22 26 + Tsp4CI* ACNGT 24 23 . . 30 33 + TspEI AATT 29 33 . . 31 36 + VspI ATTAAT 32 34 . . 32 35 + MseI TTAA 32 34 . . 47 52 + NspI RCATGY 51 47 . . 47 52 + Cac8I GCNNGC 49 49 . . 47 52 + SphI GCATGC 51 47 . . 48 51 + FatI CATG 47 51 . . 48 51 + NlaIII CATG 51 47 . . 48 51 + CviAII CATG 48 50 . . 54 57 + TspEI AATT 53 57 . . 56 60 - TspDTI ATGAA 46 44 . . 64 68 + DdeI CTNAG 64 67 . . 70 74 + MboII GAAGA 82 81 . . 73 77 - Hin4II* CCTTC 67 66 . . 87 91 - BsrI ACTGG 87 85 . . 86 91 - BfiI ACTGGG 81 80 . . 96 100 + TfiI GAWTC 96 99 . . 96 100 + HinfI GANTC 96 99 . . 101 106 + TsoI TARCCA 117 115 . . 104 108 + BccI CCATC 112 113 . . 113 116 + TspEI AATT 112 116 . . 115 118 + MseI TTAA 115 117 . . 122 127 + TsoI TARCCA 138 136 . . 123 126 + CviJI RGCY 124 124 . . 130 133 + FatI CATG 129 133 . . 130 133 + NlaIII CATG 133 129 . . 130 133 + CviAII CATG 130 132 . . 133 139 + BstEII GGTNACC 133 138 . . 134 138 + MaeIII GTNAC 133 138 . . 132 137 - TsoI TARCCA 122 120 . . 137 140 + SetI ASST 140 136 . . 138 141 + MnlI CCTC 148 147 . . 139 143 + DdeI CTNAG 139 142 . . 139 143 + BspCNI CTCAG 152 150 . . 139 143 + BseMII CTCAG 153 151 . . 140 144 + Hpy188I TCNGA 142 141 . . 151 156 + DsaI* CCRYGG 151 155 . . 151 156 + StyI CCWWGG 151 155 . . 151 156 + NcoI CCATGG 151 155 . . 151 156 + SecI* CCNNGG 151 155 . . 152 155 + FatI CATG 151 155 . . 152 155 + NlaIII CATG 155 151 . . 152 155 + CviAII CATG 152 154 . . 156 159 + Csp6I GTAC 156 158 . . 156 159 + RsaI GTAC 157 157 . . 161 166 + BspLU11I* ACATGT 161 165 . . 161 166 + NspI RCATGY 165 161 . . 161 166 + AflIII ACRYGT 161 165 . . 162 165 + FatI CATG 161 165 . . 162 165 + NlaIII CATG 165 161 . . 162 165 + CviAII CATG 162 164 . . 168 172 - Hin4II* CCTTC 162 161 . . 186 189 + DpnI GATC 187 187 . . 186 189 + BstKTI GATC 188 186 . . 186 189 + MboI GATC 185 189 . . 194 198 + MaeIII GTNAC 193 198 . . 200 203 + SetI ASST 203 199 . . 200 203 + CviJI RGCY 201 201 . . 200 203 + AluI AGCT 201 201 . . 211 214 + TspEI AATT 210 214 . . 238 242 + BmgT120I GGNCC 239 240 . . 238 242 + AsuI* GGNCC 238 241 . . 239 242 + CviJI RGCY 240 240 . . 239 242 + HaeIII GGCC 240 240 . . 240 245 + Cfr10I RCCGGY 240 244 . . 241 244 + HpaII CCGG 241 243 . . 243 247 + HphI GGTGA 255 254 . . 247 250 + SetI ASST 250 246 . . 246 249 - MnlI CCTC 239 238 . . 253 258 + BtsI GCAGTG 260 258 . . 254 260 + TspRI CASTGNN 260 251 . . 277 282 + Hpy178III* TCNNGA 278 280 . . 281 285 + MboII GAAGA 293 292 . . 295 300 - TsoI TARCCA 285 283 . . 304 308 + HinfI GANTC 304 307 . . 304 308 - PleI GAGTC 298 299 . . 304 308 - MlyI GAGTC 298 298 . . 307 311 - HphI GGTGA 299 298 . . 318 321 - MnlI CCTC 311 310 . . 322 327 + KpnI GGTACC 326 322 . . 322 327 + NlaIV GGNNCC 324 324 . . 322 327 + HgiCI* GGYRCC 322 326 . . 322 327 + Acc65I GGTACC 322 326 . . 323 326 + Csp6I GTAC 323 325 . . 323 326 + RsaI GTAC 324 324 . . 325 329 + Tsp4CI* ACNGT 327 326 . . 340 344 + Tsp45I GTSAC 339 344 . . 340 344 + MaeIII GTNAC 339 344 . . 347 350 + SetI ASST 350 346 . . 357 362 + ClaI ATCGAT 358 360 . . 358 361 + TaqI TCGA 358 360 . . 359 364 + McrI* CGRYCG 362 360 . . 359 364 + PvuI CGATCG 362 360 . . 360 363 + DpnI GATC 361 361 . . 360 363 + BstKTI GATC 362 360 . . 360 363 + MboI GATC 359 363 . . 362 365 + TaqI TCGA 362 364 . . 364 368 + MboII GAAGA 376 375 . . 364 369 + BbvII* GAAGAC 371 375 . . 375 378 + TspEI AATT 374 378 . . 380 383 + DpnI GATC 381 381 . . 380 383 + BstKTI GATC 382 380 . . 380 383 + MboI GATC 379 383 . . 382 386 + Hpy188I TCNGA 384 383 . . 385 389 + HinfI GANTC 385 388 . . 385 389 - PleI GAGTC 379 380 . . 385 389 - MlyI GAGTC 379 379 . . 389 393 + DdeI CTNAG 389 392 . . 389 393 + BspCNI CTCAG 402 400 . . 389 393 + BseMII CTCAG 403 401 . . 394 397 + MseI TTAA 394 396 . . 397 401 + TspDTI ATGAA 412 410 . . 399 404 + ApoI RAATTY 399 403 . . 400 403 + TspEI AATT 399 403 . . 408 412 + Tsp4CI* ACNGT 410 409 . . 415 419 + BccI CCATC 423 424 . . 415 425 + BsiYI* CCNNNNNNNGG 421 418 . . 415 425 + PflMI CCANNNNNTGG 421 418 . . 416 420 - FokI GGATG 402 406 . . 416 420 - BseGI GGATG 415 413 . . 429 432 + SetI ASST 432 428 . . 430 433 + MnlI CCTC 440 439 . . 425 430 - BciVI GTATCC 419 418 . . 430 436 + SauI* CCTNAGG 431 434 . . 431 435 + DdeI CTNAG 431 434 . . 431 435 + BspCNI CTCAG 444 442 . . 431 435 + BseMII CTCAG 445 443 . . 432 437 + Hpy178III* TCNNGA 433 435 . . 435 439 + FokI GGATG 448 452 . . 435 439 + BseGI GGATG 441 439 . . 448 452 + MboII GAAGA 460 459 . . 452 455 + SetI ASST 455 451 . . 452 455 + CviJI RGCY 453 453 . . 452 455 + AluI AGCT 453 453 . . 448 453 - Ksp632I* CTCTTC 443 446 . . 448 454 - SapI GCTCTTC 443 446 . . 461 464 + TspEI AATT 460 464 . . 478 492 + XcmI CCANNNNNNNNNTGG 485 484 . . 486 496 + BsiYI* CCNNNNNNNGG 492 489 . . 492 496 - Hin4II* CCTTC 486 485 . . 496 500 - Hin4II* CCTTC 490 489 . . 503 506 + SetI ASST 506 502 . . 504 509 + Eco31I GGTCTC 510 514 . . 505 509 + BsmAI GTCTC 510 514 . . 511 516 + NlaIV GGNNCC 513 513 . . 511 516 + HgiCI* GGYRCC 511 515 . . 517 522 + Hpy166II GTNNAC 519 519 . . 514 518 - BceAI ACGGC 499 501 . . 522 527 + DsaI* CCRYGG 522 526 . . 522 527 + SecI* CCNNGG 522 526 . . 523 532 + MslI CAYNNNNRTG 527 527 . . 523 532 + OliI CACNNNNGTG 527 527 . . 524 528 + Tsp4CI* ACNGT 526 525 . . 529 533 + HphI GGTGA 541 540 . . 540 543 + DpnI GATC 541 541 . . 540 543 + BstKTI GATC 542 540 . . 540 543 + MboI GATC 539 543 . . 540 544 - BinI* GGATC 534 535 . . 569 574 + SspI AATATT 571 571 . . 587 590 + TspEI AATT 586 590 . . 592 601 + MslI CAYNNNNRTG 596 596 . . 594 599 + Hpy178III* TCNNGA 595 597 . . 592 596 - FokI GGATG 578 582 . . 592 596 - BseGI GGATG 591 589 . . 612 617 + SplI* CGTACG 612 616 . . 613 616 + Csp6I GTAC 613 615 . . 613 616 + RsaI GTAC 614 614 . . 614 619 + SnaBI TACGTA 616 616 . . 614 619 + BsaAI YACGTR 616 616 . . 610 614 - BceAI ACGGC 595 597 . . 615 618 + TaiI ACGT 618 614 . . 615 618 + SetI ASST 618 614 . . 615 618 + MaeII ACGT 615 617 . . 625 629 + TfiI GAWTC 625 628 . . 625 629 + HinfI GANTC 625 628 . . 628 632 + Hpy188I TCNGA 630 629 . . 649 652 + MseI TTAA 649 651 . . 659 662 + MseI TTAA 659 661 . . 667 671 + Hin4II* CCTTC 677 676 . . 670 674 + Hpy188I TCNGA 672 671 . . 678 681 + SetI ASST 681 677 . . 685 690 + KpnI GGTACC 689 685 . . 685 690 + NlaIV GGNNCC 687 687 . . 685 690 + HgiCI* GGYRCC 685 689 . . 685 690 + Acc65I GGTACC 685 689 . . 686 689 + Csp6I GTAC 686 688 . . 686 689 + RsaI GTAC 687 687 . . 686 698 - BarI GAAGNNNNNNTAC 710 705 678 673 699 702 + SetI ASST 702 698 . . 695 700 - Eco57MI CTGRAG 680 678 . . 695 700 - Eco57I CTGAAG 680 678 . . 704 707 + Csp6I GTAC 704 706 . . 704 707 + RsaI GTAC 705 705 . . 709 713 + TfiI GAWTC 709 712 . . 709 713 + HinfI GANTC 709 712 . . 724 729 + NspI RCATGY 728 724 . . 725 728 + FatI CATG 724 728 . . 725 728 + NlaIII CATG 728 724 . . 725 728 + CviAII CATG 725 727 . . 729 733 + Hpy188I TCNGA 731 730 . . 730 734 + DdeI CTNAG 730 733 . . 733 738 + HaeII RGCGCY 737 733 . . 733 738 + Eco47III AGCGCT 735 735 . . 734 737 + Hin6I GCGC 734 736 . . 734 737 + GlaI GCGC 735 735 . . 730 734 - BspCNI CTCAG 722 720 . . 730 734 - BseMII CTCAG 721 719 . . 734 737 + HhaI GCGC 736 734 . . 750 754 + Hin4II* CCTTC 760 759 . . 754 763 + MslI CAYNNNNRTG 758 758 . . 752 756 - TspDTI ATGAA 742 740 . . 764 768 + TfiI GAWTC 764 767 . . 764 768 + HinfI GANTC 764 767 . . 767 771 - HphI GGTGA 759 758 . . 783 786 + TspEI AATT 782 786 . . 793 797 + MaeIII GTNAC 792 797 . . 798 801 + CviRI* TGCA 799 799 . . 801 806 + NspI RCATGY 805 801 . . 802 805 + FatI CATG 801 805 . . 802 805 + NlaIII CATG 805 801 . . 802 805 + CviAII CATG 802 804 . . 797 802 - BsgI GTGCAG 782 780 . . 805 810 - GsaI CCCAGC 809 805 . . 805 810 - BseYI CCCAGC 805 809 . . 819 822 + SetI ASST 822 818 . . 819 822 + CviJI RGCY 820 820 . . 819 822 + AluI AGCT 820 820 . . 820 824 + BlsI GCNGC 822 821 . . 820 824 + BisI GCNGC 821 822 . . 820 824 + TseI GCWGC 820 823 . . 820 824 - BbvI GCAGC 807 811 . . 837 840 + FatI CATG 836 840 . . 837 840 + NlaIII CATG 840 836 . . 837 840 + CviAII CATG 837 839 . . 838 842 + TspDTI ATGAA 853 851 . . 842 845 + SetI ASST 845 841 . . 842 845 + CviJI RGCY 843 843 . . 842 845 + AluI AGCT 843 843 . . 850 855 + BsaAI YACGTR 852 852 . . 850 855 + PmaCI CACGTG 852 852 . . 851 854 + TaiI ACGT 854 850 . . 851 854 + SetI ASST 854 850 . . 851 854 + MaeII ACGT 851 853 . . 857 860 + SetI ASST 860 856 . . 857 860 + CviJI RGCY 858 858 . . 857 860 + AluI AGCT 858 858 . . 859 862 + MaeI CTAG 859 861 . . 861 866 + XhoII RGATCY 861 865 . . 862 865 + DpnI GATC 863 863 . . 862 865 + BstKTI GATC 864 862 . . 862 865 + MboI GATC 861 865 . . 864 869 + MmeI TCCRAC 889 887 . . 862 866 - BinI* GGATC 856 857 . . 880 885 + Hpy166II GTNNAC 882 882 . . 882 886 + BmgT120I GGNCC 883 884 . . 882 886 + AvaII GGWCC 882 885 . . 882 886 + AsuI* GGNCC 882 885 . . 884 887 + SetI ASST 887 883 . . 885 889 + BssKI CCNGG 884 889 . . 885 889 + BseBI CCWGG 886 887 . . 885 890 + SecI* CCNNGG 885 889 . . 885 889 + ScrFI CCNGG 886 887 . . 885 889 + EcoRII CCWGG 884 889 . . 896 907 + BdaI TGANNNNNNTCA 885 883 919 917 897 906 + XmnI GAANNNNTTC 901 901 . . 901 906 + ApoI RAATTY 901 905 . . 902 905 + TspEI AATT 901 905 . . 896 907 - BdaI TGANNNNNNTCA 919 917 885 883 904 908 - TspDTI ATGAA 894 892 . . 921 924 + TspEI AATT 920 924 . . 927 931 + BsrI ACTGG 932 930 . . 934 939 + NlaIV GGNNCC 936 936 . . 938 941 + AciI CCGC 938 940 . . 938 943 + BsrBI CCGCTC 940 940 . . 952 955 + MnlI CCTC 962 961 . . 955 958 + FatI CATG 954 958 . . 955 958 + NlaIII CATG 958 954 . . 955 958 + CviAII CATG 955 957 . . 976 980 - Hin4II* CCTTC 970 969 . . 1017 1022 + AflIII ACRYGT 1017 1021 . . 1018 1023 + BtrI CACGTC 1020 1020 . . 1019 1022 + TaiI ACGT 1022 1018 . . 1019 1022 + SetI ASST 1022 1018 . . 1019 1022 + MaeII ACGT 1019 1021 . . 1020 1024 + Hpy99I CGWCG 1024 1019 . . 1021 1026 + SalI GTCGAC 1021 1025 . . 1021 1026 + Hpy166II GTNNAC 1023 1023 . . 1021 1026 + HindII GTYRAC 1023 1023 . . 1021 1026 + AccI GTMKAC 1022 1024 . . 1022 1025 + TaqI TCGA 1022 1024 . . 1024 1033 + PshAI GACNNNNGTC 1028 1028 . . 1025 1029 + Tsp4CI* ACNGT 1027 1026 . . 1033 1036 + MaeI CTAG 1033 1035 . . 1035 1038 + SetI ASST 1038 1034 . . 1036 1041 + NlaIV GGNNCC 1038 1038 . . 1050 1054 - TspDTI ATGAA 1040 1038 . . 1065 1068 + CviJI RGCY 1066 1066 . . 1070 1074 + MaeIII GTNAC 1069 1074 . . 1068 1073 - TsoI TARCCA 1058 1056 . . 1093 1104 + DrdI GACNNNNNNGTC 1099 1097 . . 1100 1104 + PleI GAGTC 1108 1109 . . 1100 1104 + MlyI GAGTC 1109 1109 . . 1100 1104 + HinfI GANTC 1100 1103 . . 1104 1109 + SmaI CCCGGG 1106 1106 . . 1104 1109 + Cfr9I CCCGGG 1104 1108 . . 1104 1108 + BssKI CCNGG 1103 1108 . . 1104 1109 + SecI* CCNNGG 1104 1108 . . 1104 1109 + BmeT110I CYCGRG 1105 1107 . . 1104 1108 + ScrFI CCNGG 1105 1106 . . 1104 1108 + CauII* CCSGG 1105 1106 . . 1104 1109 + AvaI CYCGRG 1104 1108 . . 1105 1109 + BssKI CCNGG 1104 1109 . . 1105 1108 + HpaII CCGG 1105 1107 . . 1105 1109 + ScrFI CCNGG 1106 1107 . . 1105 1109 + CauII* CCSGG 1106 1107 . . 1102 1106 - BslFI GGGAC 1087 1091 . . 1109 1113 + PleI GAGTC 1117 1118 . . 1109 1113 + MlyI GAGTC 1118 1118 . . 1109 1113 + HinfI GANTC 1109 1112 . . 1111 1115 + Tsp45I GTSAC 1110 1115 . . 1111 1115 + MaeIII GTNAC 1110 1115 . . 1114 1117 + SetI ASST 1117 1113 . . 1115 1118 + MnlI CCTC 1125 1124 . . 1112 1116 - HphI GGTGA 1104 1103 . . 1124 1127 + FatI CATG 1123 1127 . . 1124 1127 + NlaIII CATG 1127 1123 . . 1124 1127 + CviAII CATG 1124 1126 . . 1127 1131 + Tsp45I GTSAC 1126 1131 . . 1127 1131 + MaeIII GTNAC 1126 1131 . . 1135 1144 + MslI CAYNNNNRTG 1139 1139 . . 1149 1152 + CviRI* TGCA 1150 1150 . . 1159 1162 + CviJI RGCY 1160 1160 . . 1169 1174 + PsiI TTATAA 1171 1171 . . 1196 1201 + BsmI GAATGC 1202 1200 . . 1198 1203 + EcoT22I ATGCAT 1202 1198 . . 1199 1202 + CviRI* TGCA 1200 1200 . . 1200 1204 + SfaNI GCATC 1209 1213 . . 1210 1215 + SnaBI TACGTA 1212 1212 . . 1210 1215 + BsaAI YACGTR 1212 1212 . . 1211 1214 + TaiI ACGT 1214 1210 . . 1211 1214 + SetI ASST 1214 1210 . . 1211 1214 + MaeII ACGT 1211 1213 . . 1228 1232 + BsrI ACTGG 1233 1231 . . 1232 1235 - AciI CCGC 1232 1234 . . 1232 1236 - FauI CCCGC 1225 1227 . . 1243 1246 + CviJI RGCY 1244 1244 . . 1261 1266 + NlaIV GGNNCC 1263 1263 . . 1262 1266 + BmgT120I GGNCC 1263 1264 . . 1262 1266 + AvaII GGWCC 1262 1265 . . 1262 1266 + AsuI* GGNCC 1262 1265 . . 1265 1269 + BssKI CCNGG 1264 1269 . . 1265 1269 + BseBI CCWGG 1266 1267 . . 1265 1269 + ScrFI CCNGG 1266 1267 . . 1265 1269 + EcoRII CCWGG 1264 1269 . . 1267 1272 + StuI AGGCCT 1269 1269 . . 1268 1271 + CviJI RGCY 1269 1269 . . 1268 1271 + HaeIII GGCC 1269 1269 . . 1270 1274 + BssKI CCNGG 1269 1274 . . 1270 1274 + BseBI CCWGG 1271 1272 . . 1270 1274 + ScrFI CCNGG 1271 1272 . . 1270 1274 + EcoRII CCWGG 1269 1274 . . 1287 1290 + SetI ASST 1290 1286 . . 1295 1299 + BmgT120I GGNCC 1296 1297 . . 1295 1299 + AsuI* GGNCC 1295 1298 . . 1296 1299 + CviJI RGCY 1297 1297 . . 1296 1299 + HaeIII GGCC 1297 1297 . . 1306 1311 + Hpy166II GTNNAC 1308 1308 . . 1306 1311 + HindII GTYRAC 1308 1308 . . 1311 1314 + FatI CATG 1310 1314 . . 1311 1314 + NlaIII CATG 1314 1310 . . 1311 1314 + CviAII CATG 1311 1313 . . 1315 1319 + HphI GGTGA 1327 1326 . . 1313 1318 - TaqII CACCCA 1303 1301 . . 1326 1329 + Csp6I GTAC 1326 1328 . . 1326 1329 + RsaI GTAC 1327 1327 . . 1333 1337 + PleI GAGTC 1341 1342 . . 1333 1337 + MlyI GAGTC 1342 1342 . . 1333 1337 + HinfI GANTC 1333 1336 . . 1355 1360 + BsgI GTGCAG 1376 1374 . . 1356 1359 + CviRI* TGCA 1357 1357 . . 1357 1361 + BlsI GCNGC 1359 1358 . . 1357 1361 + BisI GCNGC 1358 1359 . . 1357 1361 + BbvI GCAGC 1369 1373 . . 1357 1361 + TseI GCWGC 1357 1360 . . 1360 1365 + BsrDI GCAATG 1367 1365 . . 1363 1367 + TspDTI ATGAA 1378 1376 . . 1369 1374 + AhaIII* TTTAAA 1371 1371 . . 1370 1373 + MseI TTAA 1370 1372 . . 1382 1388 + PfoI TCCNGGA 1382 1387 . . 1383 1387 + BssKI CCNGG 1382 1387 . . 1383 1387 + BseBI CCWGG 1384 1385 . . 1383 1387 + ScrFI CCNGG 1384 1385 . . 1383 1387 + EcoRII CCWGG 1382 1387 . . 1380 1384 - FokI GGATG 1366 1370 . . 1380 1384 - BseGI GGATG 1379 1377 . . 1387 1396 + XmnI GAANNNNTTC 1391 1391 . . 1395 1400 + Hpy178III* TCNNGA 1396 1398 . . 1400 1403 + SetI ASST 1403 1399 . . 1421 1424 + MaeI CTAG 1421 1423 . . 1424 1434 + Hin4I GAYNNNNNVTC 1415 1410 1447 1442 1424 1434 - Hin4I GAYNNNNNVTC 1447 1442 1415 1410 1437 1441 - TspDTI ATGAA 1427 1425 . . 1444 1448 - MboII GAAGA 1436 1435 . . 1453 1456 + SetI ASST 1456 1452 . . 1474 1478 + Hpy188I TCNGA 1476 1475 . . 1477 1487 + Hin4I GAYNNNNNVTC 1468 1463 1500 1495 1477 1487 + Eam1105I GACNNNNNGTC 1482 1481 . . 1480 1483 + SetI ASST 1483 1479 . . 1477 1487 - Hin4I GAYNNNNNVTC 1500 1495 1468 1463 1495 1498 + CviJI RGCY 1496 1496 . . 1496 1501 + Cac8I GCNNGC 1498 1498 . . 1500 1511 + AlfI GCANNNNNNTGC 1489 1487 1523 1521 1501 1510 + MslI CAYNNNNRTG 1505 1505 . . 1500 1511 - AlfI GCANNNNNNTGC 1523 1521 1489 1487 1520 1523 + CviJI RGCY 1521 1521 . . 1521 1526 + Cfr10I RCCGGY 1521 1525 . . 1522 1525 + HpaII CCGG 1522 1524 . . 1527 1530 + CviRI* TGCA 1528 1528 . . 1536 1539 + FatI CATG 1535 1539 . . 1536 1539 + NlaIII CATG 1539 1535 . . 1536 1539 + CviAII CATG 1536 1538 . . 1539 1542 + CviJI RGCY 1540 1540 . . 1539 1542 + HaeIII GGCC 1540 1540 . . 1541 1545 + Hin4II* CCTTC 1551 1550 . . 1544 1549 + Hpy178III* TCNNGA 1545 1547 . . 1545 1550 + BsiI* CACGAG 1545 1549 . . 1555 1560 + HaeII RGCGCY 1559 1555 . . 1555 1560 + Eco47III AGCGCT 1557 1557 . . 1556 1559 + Hin6I GCGC 1556 1558 . . 1556 1559 + GlaI GCGC 1557 1557 . . 1556 1559 + HhaI GCGC 1558 1556 . . 1561 1564 + FatI CATG 1560 1564 . . 1561 1564 + NlaIII CATG 1564 1560 . . 1561 1564 + CviAII CATG 1561 1563 . . 1569 1573 + FokI GGATG 1582 1586 . . 1569 1573 + BseGI GGATG 1575 1573 . . 1574 1579 + XhoII RGATCY 1574 1578 . . 1574 1579 + BglII AGATCT 1574 1578 . . 1575 1578 + DpnI GATC 1576 1576 . . 1575 1578 + BstKTI GATC 1577 1575 . . 1575 1578 + MboI GATC 1574 1578 . . 1589 1594 + SnaBI TACGTA 1591 1591 . . 1589 1594 + BsaAI YACGTR 1591 1591 . . 1590 1593 + TaiI ACGT 1593 1589 . . 1590 1593 + SetI ASST 1593 1589 . . 1590 1593 + MaeII ACGT 1590 1592 . . 1592 1595 + Csp6I GTAC 1592 1594 . . 1592 1595 + RsaI GTAC 1593 1593 . . 1597 1601 + Tsp4CI* ACNGT 1599 1598 . . 1601 1604 + CviRI* TGCA 1602 1602 . . 1611 1615 + MboII GAAGA 1623 1622 . . 1611 1616 - Ksp632I* CTCTTC 1606 1609 . . 1611 1617 - SapI GCTCTTC 1606 1609 . . 1618 1622 + TfiI GAWTC 1618 1621 . . 1618 1622 + HinfI GANTC 1618 1621 . . 1623 1628 + ApoI RAATTY 1623 1627 . . 1624 1627 + TspEI AATT 1623 1627 . . 1633 1637 + BccI CCATC 1641 1642 . . 1634 1638 - FokI GGATG 1620 1624 . . 1634 1638 - BseGI GGATG 1633 1631 . . 1643 1647 + TspGWI ACGGA 1658 1656 . . 1646 1657 + PpiI GAACNNNNNCTC 1638 1633 1670 1665 1649 1652 + MaeI CTAG 1649 1651 . . 1651 1656 + HaeII RGCGCY 1655 1651 . . 1651 1656 + Eco47III AGCGCT 1653 1653 . . 1652 1655 + Hin6I GCGC 1652 1654 . . 1652 1655 + GlaI GCGC 1653 1653 . . 1652 1655 + HhaI GCGC 1654 1652 . . 1670 1674 + BssKI CCNGG 1669 1674 . . 1670 1673 + HpaII CCGG 1670 1672 . . 1670 1674 + ScrFI CCNGG 1671 1672 . . 1670 1674 + CauII* CCSGG 1671 1672 . . 1679 1682 + MseI TTAA 1679 1681 . . 1687 1697 + MwoI GCNNNNNNNGC 1693 1690 . . 1687 1692 + Cac8I GCNNGC 1689 1689 . . 1685 1690 - EcoP15I CAGCAG 1657 1659 . . 1695 1698 + CviRI* TGCA 1696 1696 . . 1703 1706 + TspEI AATT 1702 1706 . . 1715 1719 + TfiI GAWTC 1715 1718 . . 1715 1719 + HinfI GANTC 1715 1718 . . 1720 1723 + MaeI CTAG 1720 1722 . . 1723 1727 + HgaI GACGC 1732 1737 . . 1740 1745 + NlaIV GGNNCC 1742 1742 . . 1741 1745 + BmgT120I GGNCC 1742 1743 . . 1741 1745 + AvaII GGWCC 1741 1744 . . 1741 1745 + AsuI* GGNCC 1741 1744 . . 1743 1749 + PfoI TCCNGGA 1743 1748 . . 1744 1748 + BssKI CCNGG 1743 1748 . . 1744 1748 + BseBI CCWGG 1745 1746 . . 1744 1748 + ScrFI CCNGG 1745 1746 . . 1744 1748 + EcoRII CCWGG 1743 1748 . . 1750 1753 - MnlI CCTC 1743 1742 . . 1752 1757 - BciVI GTATCC 1746 1745 . . 1757 1760 + AciI CCGC 1757 1759 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 322 363 1085 # # AccI: # [GTMKAC] # 748 1022 # # AciI: # [CCGC] # 13 294 525 938 # # AflIII: # [ACRYGT] # 161 753 856 # # AhaIII*: # [TTTAAA] # 399 1371 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 247 1489 # # AluI: # [AGCT] # 15 23 201 252 367 912 # # ApoI: # [RAATTY] # 147 399 502 722 # # AsuI*: # [GGNCC] # 29 33 238 380 446 644 # # AvaI: # [CYCGRG] # 666 1104 # # AvaII: # [GGWCC] # 29 380 479 882 # # BarI: # [GAAGNNNNNNTAC] # 32 678 1060 # # BbvI: # [GCAGC] # 401 562 807 # # BbvII*: # [GAAGAC] # 371 1399 # # BccI: # [CCATC] # 112 129 311 1218 # # BceAI: # [ACGGC] # 96 499 1175 # # BciVI: # [GTATCC] # 24 419 1327 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 851 885 # # BfiI: # [ACTGGG] # 81 1689 # # BglII: # [AGATCT] # 196 1574 # # BinI*: # [GGATC] # 322 534 914 # # BisI: # [GCNGC] # 412 537 821 # # BlsI: # [GCNGC] # 411 537 822 # # BmeT110I: # [CYCGRG] # 665 1105 # # BmgT120I: # [GGNCC] # 28 33 239 380 446 644 # # BsaAI: # [YACGTR] # 179 236 360 379 616 # # BseBI: # [CCWGG] # 5 25 113 361 380 886 # # BseGI: # [GGATG] # 26 58 137 150 196 415 # 788 # # BseMII: # [CTCAG] # 42 153 250 276 1049 # # BseYI: # [CCCAGC] # 805 965 # # BsgI: # [GTGCAG] # 394 594 782 # # BsiI*: # [CACGAG] # 225 1545 # # BsiYI*: # [CCNNNNNNNGG] # 71 421 1278 # # BslFI: # [GGGAC] # 683 1087 # # BsmAI: # [GTCTC] # 510 1260 # # BsmI: # [GAATGC] # 568 1202 # # BspCNI: # [CTCAG] # 42 152 250 278 1048 # # BspLU11I*: # [ACATGT] # 161 1609 # # BsrBI: # [CCGCTC] # 830 940 # # BsrDI: # [GCAATG] # 403 1367 # # BsrI: # [ACTGG] # 87 301 537 845 # # BssKI: # [CCNGG] # 1 5 27 74 113 160 # 219 287 884 # # BstEII: # [GGTNACC] # 133 1637 # # BstKTI: # [GATC] # 20 160 174 188 193 322 # 713 # # BtrI: # [CACGTC] # 750 1020 # # BtsI: # [GCAGTG] # 260 1510 # # Cac8I: # [GCNNGC] # 49 81 191 1449 # # CauII*: # [CCSGG] # 1 99 565 1105 # # Cfr10I: # [RCCGGY] # 240 249 1281 # # Cfr9I: # [CCCGGG] # 666 1104 # # ClaI: # [ATCGAT] # 358 1412 # # Csp6I: # [GTAC] # 18 73 156 167 178 266 # 290 622 # # CviAII: # [CATG] # 10 22 25 35 48 77 # 82 118 169 187 209 225 # 563 # # CviJI: # [RGCY] # 15 19 23 25 25 28 # 39 77 84 94 124 199 # 208 213 230 367 # # CviRI*: # [TGCA] # 50 74 74 94 157 171 # 351 799 # # DdeI: # [CTNAG] # 42 64 75 250 299 1040 # # DpnI: # [GATC] # 20 160 174 187 194 322 # 713 # # DrdI: # [GACNNNNNNGTC] # 671 1099 # # DsaI*: # [CCRYGG] # 151 371 1248 # # Eam1105I: # [GACNNNNNGTC] # 288 1482 # # Eco31I: # [GGTCTC] # 510 1260 # # Eco47III: # [AGCGCT] # 96 117 735 822 # # Eco57I: # [CTGAAG] # 680 1090 # # Eco57MI: # [CTGRAG] # 680 1090 # # EcoP15I: # [CAGCAG] # 113 1657 # # EcoRII: # [CCWGG] # 5 27 113 361 380 884 # # EcoT22I: # [ATGCAT] # 568 1202 # # FatI: # [CATG] # 10 22 25 35 47 77 # 82 118 169 187 210 225 # 563 # # FauI: # [CCCGC] # 545 1225 # # FokI: # [GGATG] # 38 46 130 150 216 402 # 788 # # GlaI: # [GCGC] # 96 117 735 822 # # GsaI: # [CCCAGC] # 809 961 # # HaeII: # [RGCGCY] # 96 115 737 822 # # HaeIII: # [GGCC] # 28 230 240 243 1029 # # HgaI: # [GACGC] # 38 1732 # # HgiCI*: # [GGYRCC] # 174 189 322 1085 # # HhaI: # [GCGC] # 96 116 736 822 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 21 32 # 32 270 1415 # # Hin4II*: # [CCTTC] # 4 67 83 95 187 210 # 219 324 581 # # Hin6I: # [GCGC] # 96 118 734 822 # # HindII: # [GTYRAC] # 285 462 1023 # # HinfI: # [GANTC] # 9 55 55 81 84 96 # 97 208 224 240 285 336 # # HpaII: # [CCGG] # 100 148 241 417 864 # # HphI: # [GGTGA] # 44 218 223 242 255 345 # 443 # # Hpy166II: # [GTNNAC] # 141 285 363 462 519 # # Hpy178III*: # [TCNNGA] # 149 155 162 225 278 801 # # Hpy188I: # [TCNGA] # 42 59 142 242 246 294 # 745 # # Hpy99I: # [CGWCG] # 746 1024 # # KpnI: # [GGTACC] # 326 363 1081 # # Ksp632I*: # [CTCTTC] # 164 443 1163 # # MaeI: # [CTAG] # 50 71 174 228 388 859 # # MaeII: # [ACGT] # 168 180 192 236 379 615 # # MaeIII: # [GTNAC] # 16 41 60 133 146 277 # 453 644 # # MboI: # [GATC] # 20 160 174 185 196 322 # 713 # # MboII: # [GAAGA] # 82 83 84 147 187 211 # 976 # # McrI*: # [CGRYCG] # 362 1408 # # MlyI: # [GAGTC] # 9 81 224 298 428 730 # # MmeI: # [TCCRAC] # 881 889 # # MnlI: # [CCTC] # 27 72 91 129 148 163 # 522 618 # # MseI: # [TTAA] # 10 32 83 91 255 279 # 309 711 # # MslI: # [CAYNNNNRTG] # 69 162 265 366 381 527 # # MwoI: # [GCNNNNNNNGC] # 77 1693 # # NcoI: # [CCATGG] # 151 1619 # # NlaIII: # [CATG] # 10 22 25 35 51 77 # 82 118 169 187 206 225 # 563 # # NlaIV: # [GGNNCC] # 28 102 174 189 225 249 # 324 479 # # NspI: # [RCATGY] # 51 77 114 563 965 # # OliI: # [CACNNNNGTG] # 527 1243 # # PflMI: # [CCANNNNNTGG] # 421 1349 # # PfoI: # [TCCNGGA] # 27 361 1382 # # PleI: # [GAGTC] # 9 81 224 298 429 729 # # PmaCI: # [CACGTG] # 852 918 # # PpiI: # [GAACNNNNNCTC] # 32 100 1638 # # PshAI: # [GACNNNNGTC] # 742 1028 # # PsiI: # [TTATAA] # 599 1171 # # PvuI: # [CGATCG] # 362 1408 # # RsaI: # [GTAC] # 18 73 157 167 177 266 # 290 622 # # SalI: # [GTCGAC] # 749 1021 # # SapI: # [GCTCTTC] # 164 443 1163 # # SauI*: # [CCTNAGG] # 431 1339 # # ScrFI: # [CCNGG] # 1 5 25 74 113 160 # 219 287 886 # # SecI*: # [CCNNGG] # 151 219 363 371 666 # # SetI: # [ASST] # 6 9 16 21 23 23 # 27 27 47 51 53 63 # 63 76 79 82 97 100 # 110 112 113 120 135 140 # 177 # # SfaNI: # [GCATC] # 561 1209 # # SmaI: # [CCCGGG] # 664 1106 # # SnaBI: # [TACGTA] # 179 379 596 616 # # SphI: # [GCATGC] # 51 1719 # # SplI*: # [CGTACG] # 612 1158 # # SspI: # [AATATT] # 571 1199 # # StuI: # [AGGCCT] # 501 1269 # # StyI: # [CCWWGG] # 151 1619 # # TaiI: # [ACGT] # 168 177 192 236 379 618 # # TaqI: # [TCGA] # 4 358 660 748 # # TaqII: # [CACCCA] # 467 1303 # # TfiI: # [GAWTC] # 55 55 84 96 97 529 # 854 # # TseI: # [GCWGC] # 413 537 820 # # TsoI: # [TARCCA] # 5 16 117 147 712 773 # # Tsp45I: # [GTSAC] # 16 339 644 771 # # Tsp4CI*: # [ACNGT] # 24 83 116 171 303 501 # 572 # # TspDTI: # [ATGAA] # 41 46 49 111 146 330 # 338 343 366 # # TspEI: # [AATT] # 19 24 25 29 59 61 # 68 79 98 119 126 164 # 196 703 # # TspGWI: # [ACGGA] # 112 1658 # # TspRI: # [CASTGNN] # 260 1510 # # VspI: # [ATTAAT] # 32 1738 # # XcmI: # [CCANNNNNNNNNTGG] # 485 1285 # # XhoII: # [RGATCY] # 196 713 861 # # XmnI: # [GAANNNNTTC] # 379 490 901 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1770 # Reported_sequences: 1 # Reported_hitcount: 495 #---------------------------------------