Restriction Fragments for PHM6/YDR281C



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PHM6/YDR281C on chromosome IV from coordinates 1022321 to 1022007.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20998.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + MboII GAAGA 16 15 . . 10 13 + SetI ASST 13 9 . . 11 18 + PspXI VCTCGAGB 12 16 . . 11 14 + MnlI CCTC 21 20 . . 11 18 + AbsI CCTCGAGG 12 16 . . 12 17 + SmlI CTYRAG 12 16 . . 12 17 + XhoI CTCGAG 12 16 . . 12 17 + BmeT110I CYCGRG 13 15 . . 12 17 + AvaI CYCGRG 12 16 . . 13 16 + TaqI TCGA 13 15 . . 16 19 + SetI ASST 19 15 . . 15 18 - MnlI CCTC 8 7 . . 19 22 + CviRI* TGCA 20 20 . . 20 24 + SfaNI GCATC 29 33 . . 22 27 + ClaI ATCGAT 23 25 . . 23 26 + TaqI TCGA 23 25 . . 30 35 + TatI WGTACW 30 34 . . 31 34 + Csp6I GTAC 31 33 . . 31 34 + RsaI GTAC 32 32 . . 54 58 - Hin4II* CCTTC 48 47 . . 79 83 + Hpy188I TCNGA 81 80 . . 82 86 + HgaI GACGC 91 96 . . 100 105 + BseRI GAGGAG 115 113 . . 100 103 - MnlI CCTC 93 92 . . 104 107 + SetI ASST 107 103 . . 103 106 - MnlI CCTC 96 95 . . 107 110 + MseI TTAA 107 109 . . 102 112 - BsaXI ACNNNNNCTCC 124 121 94 91 102 113 - TstI CACNNNNNNTCC 126 121 94 89 124 128 + Tsp4CI* ACNGT 126 125 . . 137 147 + FalI AAGNNNNNCTT 128 123 160 155 141 144 + SetI ASST 144 140 . . 139 144 - BspMI ACCTGC 130 134 . . 137 147 - FalI AAGNNNNNCTT 160 155 128 123 144 148 - MboII GAAGA 136 135 . . 143 148 - BbvII* GAAGAC 136 140 . . 148 151 + AciI CCGC 148 150 . . 149 152 + FnuDII* CGCG 150 150 . . 150 153 + Hin6I GCGC 150 152 . . 150 153 + GlaI GCGC 151 151 . . 150 153 + HhaI GCGC 152 150 . . 153 167 + XcmI CCANNNNNNNNNTGG 160 159 . . 154 159 + BsiI* CACGAG 154 158 . . 180 183 + MnlI CCTC 190 189 . . 186 190 + Tsp4CI* ACNGT 188 187 . . 187 193 + TspRI CASTGNN 193 184 . . 192 197 + PsiI TTATAA 194 194 . . 200 210 + BsiYI* CCNNNNNNNGG 206 203 . . 199 203 - BceAI ACGGC 184 186 . . 205 208 + MaeI CTAG 205 207 . . 213 218 + Hpy178III* TCNNGA 214 216 . . 214 219 + SmlI CTYRAG 214 218 . . 214 219 + XhoI CTCGAG 214 218 . . 214 219 + BmeT110I CYCGRG 215 217 . . 214 219 + AvaI CYCGRG 214 218 . . 215 218 + TaqI TCGA 215 217 . . 217 220 - MnlI CCTC 210 209 . . 254 259 + TatI WGTACW 254 258 . . 254 259 + Bsp1407I TGTACA 254 258 . . 255 258 + Csp6I GTAC 255 257 . . 255 258 + RsaI GTAC 256 256 . . 257 261 + Tsp4CI* ACNGT 259 258 . . 273 276 + SetI ASST 276 272 . . 312 315 + MseI TTAA 312 314 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 12 303 # # AciI: # [CCGC] # 148 167 # # AvaI: # [CYCGRG] # 12 101 202 # # BbvII*: # [GAAGAC] # 136 179 # # BceAI: # [ACGGC] # 131 184 # # BmeT110I: # [CYCGRG] # 13 100 202 # # BsaXI: # [ACNNNNNCTCC] # 30 94 191 # # BseRI: # [GAGGAG] # 115 200 # # BsiI*: # [CACGAG] # 154 161 # # BsiYI*: # [CCNNNNNNNGG] # 109 206 # # Bsp1407I: # [TGTACA] # 61 254 # # BspMI: # [ACCTGC] # 130 185 # # ClaI: # [ATCGAT] # 23 292 # # Csp6I: # [GTAC] # 31 60 224 # # CviRI*: # [TGCA] # 20 295 # # FalI: # [AAGNNNNNCTT] # 0 0 32 128 155 # # FnuDII*: # [CGCG] # 150 165 # # GlaI: # [GCGC] # 151 164 # # HgaI: # [GACGC] # 91 224 # # HhaI: # [GCGC] # 152 163 # # Hin4II*: # [CCTTC] # 48 267 # # Hin6I: # [GCGC] # 150 165 # # Hpy178III*: # [TCNNGA] # 101 214 # # Hpy188I: # [TCNGA] # 81 234 # # MaeI: # [CTAG] # 110 205 # # MboII: # [GAAGA] # 16 120 179 # # MnlI: # [CCTC] # 3 8 13 20 72 94 # 105 # # MseI: # [TTAA] # 3 107 205 # # PsiI: # [TTATAA] # 121 194 # # PspXI: # [VCTCGAGB] # 12 303 # # RsaI: # [GTAC] # 32 59 224 # # SetI: # [ASST] # 6 13 37 39 88 132 # # SfaNI: # [GCATC] # 29 286 # # SmlI: # [CTYRAG] # 12 101 202 # # TaqI: # [TCGA] # 10 13 100 192 # # TatI: # [WGTACW] # 30 61 224 # # Tsp4CI*: # [ACNGT] # 56 62 71 126 # # TspRI: # [CASTGNN] # 122 193 # # TstI: # [CACNNNNNNTCC] # 32 94 189 # # XcmI: # [CCANNNNNNNNNTGG] # 155 160 # # XhoI: # [CTCGAG] # 12 101 202 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 315 # Reported_sequences: 1 # Reported_hitcount: 64 #---------------------------------------