Restriction Fragments for MET32/YDR253C



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The currently selected gene/sequence is :

MET32/YDR253C on chromosome IV from coordinates 964565 to 963990.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20591.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 10 + BinI* GGATC 14 15 . . 7 10 + DpnI GATC 8 8 . . 7 10 + BstKTI GATC 9 7 . . 7 10 + MboI GATC 6 10 . . 9 14 + Hpy178III* TCNNGA 10 12 . . 12 16 + FokI GGATG 25 29 . . 12 16 + BseGI GGATG 18 16 . . 16 20 + BlsI GCNGC 18 17 . . 16 20 + BisI GCNGC 17 18 . . 16 20 + TseI GCWGC 16 19 . . 13 17 - SfaNI GCATC 3 7 . . 18 21 + CviRI* TGCA 19 19 . . 16 20 - BbvI GCAGC 3 7 . . 33 36 + CviJI RGCY 34 34 . . 34 44 + MwoI GCNNNNNNNGC 40 37 . . 35 40 + SfeI* CTRYAG 35 39 . . 51 55 + FokI GGATG 64 68 . . 51 55 + BseGI GGATG 57 55 . . 61 64 + MseI TTAA 61 63 . . 78 83 + XhoII RGATCY 78 82 . . 79 82 + DpnI GATC 80 80 . . 79 82 + BstKTI GATC 81 79 . . 79 82 + MboI GATC 78 82 . . 79 83 - BinI* GGATC 73 74 . . 127 130 + SetI ASST 130 126 . . 129 133 + MaeIII GTNAC 128 133 . . 156 161 + Cfr10I RCCGGY 156 160 . . 157 160 + HpaII CCGG 157 159 . . 194 198 + MaeIII GTNAC 193 198 . . 191 194 - AciI CCGC 191 193 . . 203 206 + SetI ASST 206 202 . . 206 209 + MseI TTAA 206 208 . . 262 265 + DpnI GATC 263 263 . . 262 265 + BstKTI GATC 264 262 . . 262 265 + MboI GATC 261 265 . . 274 279 + TatI WGTACW 274 278 . . 274 279 + ScaI AGTACT 276 276 . . 275 278 + Csp6I GTAC 275 277 . . 275 278 + RsaI GTAC 276 276 . . 277 281 + BsrI ACTGG 282 280 . . 280 284 + HphI GGTGA 292 291 . . 291 298 + SwaI ATTTAAAT 294 294 . . 292 297 + AhaIII* TTTAAA 294 294 . . 293 296 + MseI TTAA 293 295 . . 303 316 + AjuI GAANNNNNNNTTGG 295 290 327 322 316 321 + ApoI RAATTY 316 320 . . 317 320 + TspEI AATT 316 320 . . 312 317 - MmeI TCCRAC 293 291 . . 322 327 + Hpy178III* TCNNGA 323 325 . . 326 329 + DpnI GATC 327 327 . . 326 329 + BstKTI GATC 328 326 . . 326 329 + MboI GATC 325 329 . . 331 335 + Hpy188I TCNGA 333 332 . . 341 345 - Hin4II* CCTTC 335 334 . . 352 362 + FalI AAGNNNNNCTT 343 338 375 370 352 362 - FalI AAGNNNNNCTT 375 370 343 338 385 388 + MnlI CCTC 395 394 . . 385 395 + BsiYI* CCNNNNNNNGG 391 388 . . 399 402 + SetI ASST 402 398 . . 405 408 + CviRI* TGCA 406 406 . . 416 421 + EcoT22I ATGCAT 420 416 . . 417 420 + CviRI* TGCA 418 418 . . 415 419 - SfaNI GCATC 405 409 . . 440 446 + TspRI CASTGNN 446 437 . . 440 449 + MslI CAYNNNNRTG 444 444 . . 445 450 + BspLU11I* ACATGT 445 449 . . 445 450 + NspI RCATGY 449 445 . . 445 450 + AflIII ACRYGT 445 449 . . 446 449 + FatI CATG 445 449 . . 446 449 + NlaIII CATG 449 445 . . 446 449 + CviAII CATG 446 448 . . 467 470 + TspEI AATT 466 470 . . 478 481 - AciI CCGC 478 480 . . 478 482 - FauI CCCGC 471 473 . . 484 488 + HphI GGTGA 496 495 . . 487 490 - MnlI CCTC 480 479 . . 497 501 + TspDTI ATGAA 512 510 . . 500 503 + TspEI AATT 499 503 . . 506 517 + BdaI TGANNNNNNTCA 495 493 529 527 506 517 - BdaI TGANNNNNNTCA 529 527 495 493 523 528 + MmeI TCCRAC 548 546 . . 533 537 - TspDTI ATGAA 523 521 . . 540 545 + Hpy178III* TCNNGA 541 543 . . 563 573 + MwoI GCNNNNNNNGC 569 566 . . 571 574 + CviJI RGCY 572 572 . . #--------------------------------------- # # # AciI: # [CCGC] # 98 191 287 # # AflIII: # [ACRYGT] # 131 445 # # AhaIII*: # [TTTAAA] # 282 294 # # AjuI: # [GAANNNNNNNTTGG] # 32 249 295 # # ApoI: # [RAATTY] # 260 316 # # BbvI: # [GCAGC] # 3 573 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 47 495 # # BinI*: # [GGATC] # 14 59 503 # # BisI: # [GCNGC] # 17 559 # # BlsI: # [GCNGC] # 18 558 # # BseGI: # [GGATG] # 18 39 519 # # BsiYI*: # [CCNNNNNNNGG] # 185 391 # # BspLU11I*: # [ACATGT] # 131 445 # # BsrI: # [ACTGG] # 282 294 # # BstKTI: # [GATC] # 9 64 72 183 248 # # Cfr10I: # [RCCGGY] # 156 420 # # Csp6I: # [GTAC] # 275 301 # # CviAII: # [CATG] # 130 446 # # CviJI: # [RGCY] # 4 34 538 # # CviRI*: # [TGCA] # 12 19 158 387 # # DpnI: # [GATC] # 8 64 72 183 249 # # EcoT22I: # [ATGCAT] # 156 420 # # FalI: # [AAGNNNNNCTT] # 0 0 32 201 343 # # FatI: # [CATG] # 131 445 # # FauI: # [CCCGC] # 105 471 # # FokI: # [GGATG] # 25 39 512 # # Hin4II*: # [CCTTC] # 241 335 # # HpaII: # [CCGG] # 157 419 # # HphI: # [GGTGA] # 80 204 292 # # Hpy178III*: # [TCNNGA] # 10 35 218 313 # # Hpy188I: # [TCNGA] # 243 333 # # MaeIII: # [GTNAC] # 65 128 383 # # MboI: # [GATC] # 6 64 72 183 251 # # MmeI: # [TCCRAC] # 28 255 293 # # MnlI: # [CCTC] # 85 96 395 # # MseI: # [TTAA] # 61 87 145 283 # # MslI: # [CAYNNNNRTG] # 132 444 # # MwoI: # [GCNNNNNNNGC] # 7 40 529 # # NlaIII: # [CATG] # 127 449 # # NspI: # [RCATGY] # 127 449 # # RsaI: # [GTAC] # 276 300 # # ScaI: # [AGTACT] # 276 300 # # SetI: # [ASST] # 76 130 174 196 # # SfaNI: # [GCATC] # 3 171 402 # # SfeI*: # [CTRYAG] # 35 541 # # SwaI: # [ATTTAAAT] # 282 294 # # TatI: # [WGTACW] # 274 302 # # TseI: # [GCWGC] # 16 560 # # TspDTI: # [ATGAA] # 11 53 512 # # TspEI: # [AATT] # 33 77 150 316 # # TspRI: # [CASTGNN] # 130 446 # # XhoII: # [RGATCY] # 78 498 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 576 # Reported_sequences: 1 # Reported_hitcount: 85 #---------------------------------------