Restriction Fragments for YDR250C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YDR250C on chromosome IV from coordinates 960361 to 960086.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14310.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 7 10 + CviJI RGCY 8 8 . . 7 10 + HaeIII GGCC 8 8 . . 9 13 + BssKI CCNGG 8 13 . . 9 13 + BseBI CCWGG 10 11 . . 9 13 + ScrFI CCNGG 10 11 . . 9 13 + EcoRII CCWGG 8 13 . . 12 16 + HphI GGTGA 24 23 . . 25 29 + TspDTI ATGAA 40 38 . . 27 36 + XmnI GAANNNNTTC 31 31 . . 80 83 + TaqI TCGA 80 82 . . 93 96 + SetI ASST 96 92 . . 100 103 + FatI CATG 99 103 . . 100 103 + NlaIII CATG 103 99 . . 100 103 + CviAII CATG 100 102 . . 98 102 - TspDTI ATGAA 88 86 . . 119 123 - TspDTI ATGAA 109 107 . . 125 128 + MaeI CTAG 125 127 . . 128 132 + BsmAI GTCTC 133 137 . . 129 134 + Hpy178III* TCNNGA 130 132 . . 130 135 + SmlI CTYRAG 130 134 . . 130 135 + XhoI CTCGAG 130 134 . . 130 135 + BmeT110I CYCGRG 131 133 . . 130 135 + AvaI CYCGRG 130 134 . . 131 134 + TaqI TCGA 131 133 . . 131 136 + Hpy178III* TCNNGA 132 134 . . 139 142 + CviJI RGCY 140 140 . . 139 142 + HaeIII GGCC 140 140 . . 139 143 + BmgT120I GGNCC 140 141 . . 139 143 + AsuI* GGNCC 139 142 . . 142 152 + BsiYI* CCNNNNNNNGG 148 145 . . 149 153 + BceAI ACGGC 165 167 . . 150 155 + CfrI YGGCCR 150 154 . . 151 154 + CviJI RGCY 152 152 . . 151 154 + HaeIII GGCC 152 152 . . 153 163 + BsiYI* CCNNNNNNNGG 159 156 . . 162 165 + CviJI RGCY 163 163 . . 163 167 + BlsI GCNGC 165 164 . . 163 167 + BisI GCNGC 164 165 . . 163 167 + TseI GCWGC 163 166 . . 163 167 - BbvI GCAGC 150 154 . . 171 174 + TspEI AATT 170 174 . . 176 179 + FnuDII* CGCG 177 177 . . 182 187 + SduI GDGCHC 186 182 . . 182 187 + HgiJII* GRGCYC 186 182 . . 183 186 + CviJI RGCY 184 184 . . 199 202 + CviJI RGCY 200 200 . . 200 210 + MwoI GCNNNNNNNGC 206 203 . . 202 207 + TaqII CACCCA 218 216 . . 208 211 + SetI ASST 211 207 . . 208 211 + CviJI RGCY 209 209 . . 208 211 + AluI AGCT 209 209 . . 217 222 + TatI WGTACW 217 221 . . 217 222 + Bsp1407I TGTACA 217 221 . . 218 223 + Hpy166II GTNNAC 220 220 . . 218 221 + Csp6I GTAC 218 220 . . 218 221 + RsaI GTAC 219 219 . . 225 229 + MboII GAAGA 237 236 . . 247 253 + TspRI CASTGNN 253 244 . . 246 250 - BsrI ACTGG 246 244 . . 253 258 + BsaAI YACGTR 255 255 . . 254 257 + TaiI ACGT 257 253 . . 254 257 + SetI ASST 257 253 . . 254 257 + MaeII ACGT 254 256 . . 272 275 + FatI CATG 271 275 . . 272 275 + NlaIII CATG 275 271 . . 272 275 + CviAII CATG 272 274 . . #--------------------------------------- # # # AluI: # [AGCT] # 67 209 # # AsuI*: # [GGNCC] # 137 139 # # AvaI: # [CYCGRG] # 130 146 # # BbvI: # [GCAGC] # 126 150 # # BceAI: # [ACGGC] # 111 165 # # BisI: # [GCNGC] # 112 164 # # BlsI: # [GCNGC] # 111 165 # # BmeT110I: # [CYCGRG] # 131 145 # # BmgT120I: # [GGNCC] # 136 140 # # BsaAI: # [YACGTR] # 21 255 # # BseBI: # [CCWGG] # 10 266 # # BsiYI*: # [CCNNNNNNNGG] # 11 117 148 # # BsmAI: # [GTCTC] # 133 143 # # Bsp1407I: # [TGTACA] # 59 217 # # BsrI: # [ACTGG] # 30 246 # # BssKI: # [CCNGG] # 8 268 # # CfrI: # [YGGCCR] # 126 150 # # Csp6I: # [GTAC] # 58 218 # # CviAII: # [CATG] # 4 100 172 # # CviJI: # [RGCY] # 8 9 11 12 16 21 # 67 132 # # EcoRII: # [CCWGG] # 8 268 # # FatI: # [CATG] # 5 99 172 # # FnuDII*: # [CGCG] # 99 177 # # HaeIII: # [GGCC] # 8 12 124 132 # # HgiJII*: # [GRGCYC] # 90 186 # # HphI: # [GGTGA] # 24 252 # # Hpy166II: # [GTNNAC] # 56 220 # # Hpy178III*: # [TCNNGA] # 2 130 144 # # MaeI: # [CTAG] # 125 151 # # MaeII: # [ACGT] # 22 254 # # MboII: # [GAAGA] # 39 237 # # MwoI: # [GCNNNNNNNGC] # 70 206 # # NlaIII: # [CATG] # 1 103 172 # # RsaI: # [GTAC] # 57 219 # # ScrFI: # [CCNGG] # 10 266 # # SduI: # [GDGCHC] # 90 186 # # SetI: # [ASST] # 19 46 96 115 # # SmlI: # [CTYRAG] # 130 146 # # TaiI: # [ACGT] # 19 257 # # TaqI: # [TCGA] # 51 80 145 # # TaqII: # [CACCCA] # 58 218 # # TatI: # [WGTACW] # 59 217 # # TseI: # [GCWGC] # 113 163 # # TspDTI: # [ATGAA] # 16 21 24 48 167 # # TspEI: # [AATT] # 106 170 # # TspRI: # [CASTGNN] # 23 253 # # XhoI: # [CTCGAG] # 130 146 # # XmnI: # [GAANNNNTTC] # 31 245 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 276 # Reported_sequences: 1 # Reported_hitcount: 67 #---------------------------------------