Restriction Fragments for SIR4/YDR227W



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The currently selected gene/sequence is :

SIR4/YDR227W on chromosome IV from coordinates 917571 to 921647.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2467.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 20 25 + TaqII CACCCA 36 34 . . 28 31 + SetI ASST 31 27 . . 29 33 + BmgT120I GGNCC 30 31 . . 29 33 + AvaII GGWCC 29 32 . . 29 33 + AsuI* GGNCC 29 32 . . 45 50 + Hpy166II GTNNAC 47 47 . . 61 65 + MaeIII GTNAC 60 65 . . 58 62 - BsrI ACTGG 58 56 . . 77 82 + ApoI RAATTY 77 81 . . 78 81 + TspEI AATT 77 81 . . 81 86 + Hpy178III* TCNNGA 82 84 . . 91 95 + MboII GAAGA 103 102 . . 104 108 + TspDTI ATGAA 119 117 . . 111 115 + MboII GAAGA 123 122 . . 111 116 + BbvII* GAAGAC 118 122 . . 117 120 + SetI ASST 120 116 . . 122 127 + ApoI RAATTY 122 126 . . 123 126 + TspEI AATT 122 126 . . 136 139 + TaiI ACGT 139 135 . . 136 139 + SetI ASST 139 135 . . 136 139 + MaeII ACGT 136 138 . . 164 167 + CviJI RGCY 165 165 . . 164 167 + HaeIII GGCC 165 165 . . 168 173 + Hpy166II GTNNAC 170 170 . . 168 173 + HindII GTYRAC 170 170 . . 165 169 - BceAI ACGGC 150 152 . . 173 176 + AciI CCGC 173 175 . . 177 180 + TspEI AATT 176 180 . . 179 183 - TspDTI ATGAA 169 167 . . 184 187 + SetI ASST 187 183 . . 185 188 + MnlI CCTC 195 194 . . 189 192 + SetI ASST 192 188 . . 190 193 + MnlI CCTC 200 199 . . 187 191 - HphI GGTGA 179 178 . . 228 232 + Tsp4CI* ACNGT 230 229 . . 233 236 + CviRI* TGCA 234 234 . . 239 242 + CviJI RGCY 240 240 . . 248 251 + MnlI CCTC 258 257 . . 254 260 + TspRI CASTGNN 260 251 . . 250 254 - HphI GGTGA 242 241 . . 274 277 + TspEI AATT 273 277 . . 283 286 + MnlI CCTC 293 292 . . 294 299 + Hpy178III* TCNNGA 295 297 . . 326 336 + BsiYI* CCNNNNNNNGG 332 329 . . 327 331 + Hin4II* CCTTC 337 336 . . 330 334 - MboII GAAGA 322 321 . . 335 339 + BmgT120I GGNCC 336 337 . . 335 339 + AvaII GGWCC 335 338 . . 335 339 + AsuI* GGNCC 335 338 . . 333 338 - TaqII GACCGA 323 321 . . 343 346 + TaiI ACGT 346 342 . . 343 346 + SetI ASST 346 342 . . 343 346 + MaeII ACGT 343 345 . . 347 352 + EcoP15I CAGCAG 377 379 . . 353 357 + MboII GAAGA 365 364 . . 357 360 + TspEI AATT 356 360 . . 361 365 + TfiI GAWTC 361 364 . . 361 365 + HinfI GANTC 361 364 . . 373 377 + Hin4II* CCTTC 383 382 . . 379 382 + FatI CATG 378 382 . . 379 382 + NlaIII CATG 382 378 . . 379 382 + CviAII CATG 379 381 . . 385 390 + Cac8I GCNNGC 387 387 . . 385 390 + BmtI GCTAGC 389 385 . . 385 390 + NheI GCTAGC 385 389 . . 382 386 - SfaNI GCATC 372 376 . . 386 389 + MaeI CTAG 386 388 . . 391 394 + SetI ASST 394 390 . . 392 397 + Eco31I GGTCTC 398 402 . . 393 397 + BsmAI GTCTC 398 402 . . 389 394 - BspMI ACCTGC 380 384 . . 397 400 + MaeI CTAG 397 399 . . 425 429 + BssKI CCNGG 424 429 . . 425 429 + ScrFI CCNGG 426 427 . . 425 429 + CauII* CCSGG 426 427 . . 426 429 + HpaII CCGG 426 428 . . 429 434 + NspI RCATGY 433 429 . . 429 439 + MwoI GCNNNNNNNGC 435 432 . . 429 434 + Cac8I GCNNGC 431 431 . . 429 434 + SphI GCATGC 433 429 . . 430 433 + FatI CATG 429 433 . . 430 433 + NlaIII CATG 433 429 . . 430 433 + CviAII CATG 430 432 . . 432 437 + MstI* TGCGCA 434 434 . . 433 436 + Hin6I GCGC 433 435 . . 433 436 + GlaI GCGC 434 434 . . 433 436 + HhaI GCGC 435 433 . . 435 439 + BlsI GCNGC 437 436 . . 435 439 + BisI GCNGC 436 437 . . 435 439 + BbvI GCAGC 447 451 . . 435 439 + TseI GCWGC 435 438 . . 451 454 + SetI ASST 454 450 . . 452 456 + Hin4II* CCTTC 462 461 . . 457 460 + TspEI AATT 456 460 . . 464 467 + CviJI RGCY 465 465 . . 469 472 + Csp6I GTAC 469 471 . . 469 472 + RsaI GTAC 470 470 . . 470 475 + SnaBI TACGTA 472 472 . . 470 475 + BsaAI YACGTR 472 472 . . 471 474 + TaiI ACGT 474 470 . . 471 474 + SetI ASST 474 470 . . 471 474 + MaeII ACGT 471 473 . . 473 476 + Csp6I GTAC 473 475 . . 473 476 + RsaI GTAC 474 474 . . 475 485 + BaeI ACNNNNGTAYC 464 459 497 492 481 484 + Csp6I GTAC 481 483 . . 481 484 + RsaI GTAC 482 482 . . 486 491 + EcoRV GATATC 488 488 . . 520 525 + Hpy178III* TCNNGA 521 523 . . 531 536 + HindIII AAGCTT 531 535 . . 532 535 + SetI ASST 535 531 . . 532 535 + CviJI RGCY 533 533 . . 532 535 + AluI AGCT 533 533 . . 538 542 + DdeI CTNAG 538 541 . . 544 548 + MboII GAAGA 556 555 . . 544 549 - Ksp632I* CTCTTC 539 542 . . 573 577 + TfiI GAWTC 573 576 . . 573 577 + HinfI GANTC 573 576 . . 577 580 + MaeI CTAG 577 579 . . 602 605 + CviJI RGCY 603 603 . . 617 620 + CviJI RGCY 618 618 . . 623 626 + MseI TTAA 623 625 . . 631 635 + BinI* GGATC 639 640 . . 632 635 + DpnI GATC 633 633 . . 632 635 + BstKTI GATC 634 632 . . 632 635 + MboI GATC 631 635 . . 635 640 + EcoP15I CAGCAG 665 667 . . 646 651 + NarI GGCGCC 647 649 . . 646 651 + KasI GGCGCC 646 650 . . 646 651 + DinI GGCGCC 648 648 . . 646 651 + HaeII RGCGCY 650 646 . . 646 651 + NlaIV GGNNCC 648 648 . . 646 651 + AcyI GRCGYC 647 649 . . 646 651 + HgiCI* GGYRCC 646 650 . . 647 650 + Hin6I GCGC 647 649 . . 647 650 + GlaI GCGC 648 648 . . 647 650 + HhaI GCGC 649 647 . . 650 653 + MnlI CCTC 660 659 . . 668 673 + StyI CCWWGG 668 672 . . 668 673 + SecI* CCNNGG 668 672 . . 683 688 + Hpy178III* TCNNGA 684 686 . . 694 698 + Tsp4CI* ACNGT 696 695 . . 700 704 + MboII GAAGA 712 711 . . 712 716 + MboII GAAGA 724 723 . . 729 732 + SetI ASST 732 728 . . 748 752 + Hpy188I TCNGA 750 749 . . 751 755 + TfiI GAWTC 751 754 . . 751 755 + HinfI GANTC 751 754 . . 751 761 + Hin4I GAYNNNNNVTC 742 737 774 769 751 761 - Hin4I GAYNNNNNVTC 774 769 742 737 765 769 + Hpy188I TCNGA 767 766 . . 770 773 + SetI ASST 773 769 . . 768 772 - Hin4II* CCTTC 762 761 . . 778 783 + Cfr10I RCCGGY 778 782 . . 779 782 + HpaII CCGG 779 781 . . 781 785 + HphI GGTGA 793 792 . . 782 786 + Tsp45I GTSAC 781 786 . . 782 786 + MaeIII GTNAC 781 786 . . 785 789 + Tsp4CI* ACNGT 787 786 . . 786 792 + TspRI CASTGNN 792 783 . . 798 803 + BetI* WCCGGW 798 802 . . 799 802 + HpaII CCGG 799 801 . . 796 800 - HphI GGTGA 788 787 . . 802 807 + BseRI GAGGAG 817 815 . . 802 805 - MnlI CCTC 795 794 . . 822 828 + PfoI TCCNGGA 822 827 . . 823 827 + BssKI CCNGG 822 827 . . 823 827 + BseBI CCWGG 824 825 . . 823 827 + ScrFI CCNGG 824 825 . . 823 827 + EcoRII CCWGG 822 827 . . 828 831 + TspEI AATT 827 831 . . 831 834 + CviRI* TGCA 832 832 . . 840 845 + BsmI GAATGC 846 844 . . 877 881 + TfiI GAWTC 877 880 . . 874 877 - MnlI CCTC 867 866 . . 877 881 + HinfI GANTC 877 880 . . 906 911 + Hpy178III* TCNNGA 907 909 . . 916 920 - BsrI ACTGG 916 914 . . 923 926 + TspEI AATT 922 926 . . 939 942 + CviRI* TGCA 940 940 . . 947 950 + Csp6I GTAC 947 949 . . 947 950 + RsaI GTAC 948 948 . . 949 953 + Tsp4CI* ACNGT 951 950 . . 950 955 + McrI* CGRYCG 953 951 . . 955 959 + SfaNI GCATC 964 968 . . 960 963 + CviJI RGCY 961 961 . . 960 963 + HaeIII GGCC 961 961 . . 963 967 + DdeI CTNAG 963 966 . . 976 980 + BslFI GGGAC 990 994 . . 978 983 + ZraI GACGTC 980 980 . . 978 983 + AcyI GRCGYC 979 981 . . 978 983 + AatII GACGTC 982 978 . . 979 982 + TaiI ACGT 982 978 . . 979 982 + SetI ASST 982 978 . . 979 982 + MaeII ACGT 979 981 . . 984 987 + CviJI RGCY 985 985 . . 990 1000 + Hin4I GAYNNNNNVTC 981 976 1013 1008 1011 1015 + MboII GAAGA 1023 1022 . . 1014 1017 + DpnI GATC 1015 1015 . . 1014 1017 + BstKTI GATC 1016 1014 . . 1014 1017 + MboI GATC 1013 1017 . . 1021 1025 + BccI CCATC 1029 1030 . . 1044 1049 + BclI TGATCA 1044 1048 . . 1045 1048 + DpnI GATC 1046 1046 . . 1045 1048 + BstKTI GATC 1047 1045 . . 1045 1048 + MboI GATC 1044 1048 . . 1047 1052 + Hpy178III* TCNNGA 1048 1050 . . 1061 1067 + TspRI CASTGNN 1067 1058 . . 1064 1067 + CviRI* TGCA 1065 1065 . . 1061 1066 - BtsI GCAGTG 1060 1058 . . 1063 1068 - BsgI GTGCAG 1048 1046 . . 1083 1086 + CviRI* TGCA 1084 1084 . . 1086 1089 + SetI ASST 1089 1085 . . 1087 1091 + Hin4II* CCTTC 1097 1096 . . 1089 1094 + AsuII TTCGAA 1090 1092 . . 1090 1093 + TaqI TCGA 1090 1092 . . 1095 1099 + SfaNI GCATC 1104 1108 . . 1100 1103 + CviJI RGCY 1101 1101 . . 1111 1115 + Hpy188I TCNGA 1113 1112 . . 1123 1127 + TspDTI ATGAA 1138 1136 . . 1129 1133 + MboII GAAGA 1141 1140 . . 1125 1129 - Hin4II* CCTTC 1119 1118 . . 1129 1134 + BbvII* GAAGAC 1136 1140 . . 1132 1136 + HgaI GACGC 1141 1146 . . 1132 1137 + AcyI GRCGYC 1133 1135 . . 1139 1142 + SetI ASST 1142 1138 . . 1140 1143 + MnlI CCTC 1150 1149 . . 1141 1146 + SmlI CTYRAG 1141 1145 . . 1142 1147 + Hpy178III* TCNNGA 1143 1145 . . 1141 1146 - Bce83I* CTTGAG 1126 1124 . . 1148 1152 + FokI GGATG 1161 1165 . . 1148 1152 + BseGI GGATG 1154 1152 . . 1146 1149 - MnlI CCTC 1139 1138 . . 1149 1158 + BsaBI GATNNNNATC 1153 1153 . . 1149 1153 - BccI CCATC 1143 1144 . . 1159 1162 + MseI TTAA 1159 1161 . . 1164 1168 + Tsp45I GTSAC 1163 1168 . . 1164 1168 + MaeIII GTNAC 1163 1168 . . 1167 1170 + SetI ASST 1170 1166 . . 1168 1171 + MnlI CCTC 1178 1177 . . 1165 1169 - HphI GGTGA 1157 1156 . . 1177 1180 + TaqI TCGA 1177 1179 . . 1187 1192 + PvuII CAGCTG 1189 1189 . . 1187 1192 + NspBII* CMGCKG 1189 1189 . . 1188 1191 + SetI ASST 1191 1187 . . 1188 1191 + CviJI RGCY 1189 1189 . . 1188 1191 + AluI AGCT 1189 1189 . . 1191 1202 + BdaI TGANNNNNNTCA 1180 1178 1214 1212 1197 1200 + SetI ASST 1200 1196 . . 1198 1201 + MnlI CCTC 1208 1207 . . 1199 1204 + SmlI CTYRAG 1199 1203 . . 1191 1202 - BdaI TGANNNNNNTCA 1214 1212 1180 1178 1199 1204 - Bce83I* CTTGAG 1184 1182 . . 1216 1219 + CviJI RGCY 1217 1217 . . 1220 1229 + XmnI GAANNNNTTC 1224 1224 . . 1221 1226 + ApoI RAATTY 1221 1225 . . 1222 1225 + TspEI AATT 1221 1225 . . 1232 1235 + MaeI CTAG 1232 1234 . . 1238 1242 + TspDTI ATGAA 1253 1251 . . 1240 1244 + MboII GAAGA 1252 1251 . . 1274 1278 + TspDTI ATGAA 1289 1287 . . 1279 1282 + CviJI RGCY 1280 1280 . . 1279 1282 + HaeIII GGCC 1280 1280 . . 1276 1280 - Hin4II* CCTTC 1270 1269 . . 1306 1311 + Hpy178III* TCNNGA 1307 1309 . . 1308 1311 + TaqI TCGA 1308 1310 . . 1333 1337 + TspDTI ATGAA 1348 1346 . . 1344 1347 + CviRI* TGCA 1345 1345 . . 1360 1364 + HphI GGTGA 1372 1371 . . 1374 1377 + TspEI AATT 1373 1377 . . 1385 1388 + CviJI RGCY 1386 1386 . . 1385 1388 + HaeIII GGCC 1386 1386 . . 1386 1390 + TauI GCSGC 1389 1386 . . 1383 1386 - MnlI CCTC 1376 1375 . . 1386 1390 + BlsI GCNGC 1388 1387 . . 1386 1390 + BisI GCNGC 1387 1388 . . 1387 1390 + AciI CCGC 1387 1389 . . 1397 1402 + Hpy178III* TCNNGA 1398 1400 . . 1397 1402 + BspHI TCATGA 1397 1401 . . 1398 1401 + FatI CATG 1397 1401 . . 1398 1401 + NlaIII CATG 1401 1397 . . 1398 1401 + CviAII CATG 1398 1400 . . 1401 1406 + SduI GDGCHC 1405 1401 . . 1401 1406 + HgiAI* GWGCWC 1405 1401 . . 1404 1410 + TspRI CASTGNN 1410 1401 . . 1406 1411 + Eco57MI CTGRAG 1427 1425 . . 1406 1411 + Eco57I CTGAAG 1427 1425 . . 1410 1413 + SetI ASST 1413 1409 . . 1410 1413 + CviJI RGCY 1411 1411 . . 1410 1413 + AluI AGCT 1411 1411 . . 1414 1419 + BsaAI YACGTR 1416 1416 . . 1415 1418 + TaiI ACGT 1418 1414 . . 1415 1418 + SetI ASST 1418 1414 . . 1415 1418 + MaeII ACGT 1415 1417 . . 1417 1422 + Hpy166II GTNNAC 1419 1419 . . 1426 1430 + MaeIII GTNAC 1425 1430 . . 1432 1436 + MboII GAAGA 1444 1443 . . 1457 1462 + Hpy166II GTNNAC 1459 1459 . . 1459 1462 + Csp6I GTAC 1459 1461 . . 1459 1462 + RsaI GTAC 1460 1460 . . 1468 1472 - BccI CCATC 1462 1463 . . 1473 1476 + TaqI TCGA 1473 1475 . . 1491 1496 + TatI WGTACW 1491 1495 . . 1492 1495 + Csp6I GTAC 1492 1494 . . 1492 1495 + RsaI GTAC 1493 1493 . . 1501 1505 + TfiI GAWTC 1501 1504 . . 1501 1505 + HinfI GANTC 1501 1504 . . 1513 1517 + TfiI GAWTC 1513 1516 . . 1513 1517 + HinfI GANTC 1513 1516 . . 1516 1519 + TaqI TCGA 1516 1518 . . 1516 1521 + Hpy178III* TCNNGA 1517 1519 . . 1537 1541 + MaeIII GTNAC 1536 1541 . . 1543 1547 + BccI CCATC 1551 1552 . . 1552 1558 + DraII RGGNCCY 1553 1556 . . 1553 1556 + CviJI RGCY 1554 1554 . . 1553 1556 + HaeIII GGCC 1554 1554 . . 1553 1558 + NlaIV GGNNCC 1555 1555 . . 1553 1557 + BmgT120I GGNCC 1554 1555 . . 1553 1557 + AsuI* GGNCC 1553 1556 . . 1551 1554 - MnlI CCTC 1544 1543 . . 1555 1565 + BsiYI* CCNNNNNNNGG 1561 1558 . . 1556 1561 + GsaI CCCAGC 1560 1556 . . 1556 1561 + BseYI CCCAGC 1556 1560 . . 1559 1562 + SetI ASST 1562 1558 . . 1559 1562 + CviJI RGCY 1560 1560 . . 1559 1562 + AluI AGCT 1560 1560 . . 1561 1564 + MaeI CTAG 1561 1563 . . 1575 1579 + TfiI GAWTC 1575 1578 . . 1575 1579 + HinfI GANTC 1575 1578 . . 1582 1586 + TspDTI ATGAA 1597 1595 . . 1595 1598 + CviJI RGCY 1596 1596 . . 1597 1607 + EcoNI CCTNNNNNAGG 1601 1602 . . 1597 1607 + BsiYI* CCNNNNNNNGG 1603 1600 . . 1601 1605 + TspDTI ATGAA 1616 1614 . . 1603 1607 - Hin4II* CCTTC 1597 1596 . . 1628 1632 + Tsp4CI* ACNGT 1630 1629 . . 1631 1634 + Csp6I GTAC 1631 1633 . . 1631 1634 + RsaI GTAC 1632 1632 . . 1641 1645 - FokI GGATG 1627 1631 . . 1641 1645 - BseGI GGATG 1640 1638 . . 1652 1655 + TspEI AATT 1651 1655 . . 1658 1661 + CviJI RGCY 1659 1659 . . 1660 1666 + SauI* CCTNAGG 1661 1664 . . 1661 1665 + DdeI CTNAG 1661 1664 . . 1668 1671 + TspEI AATT 1667 1671 . . 1685 1688 + CviRI* TGCA 1686 1686 . . 1696 1700 + TfiI GAWTC 1696 1699 . . 1696 1700 + HinfI GANTC 1696 1699 . . 1710 1713 + CviJI RGCY 1711 1711 . . 1708 1711 - MnlI CCTC 1701 1700 . . 1712 1716 + DdeI CTNAG 1712 1715 . . 1734 1739 + AhaIII* TTTAAA 1736 1736 . . 1735 1738 + MseI TTAA 1735 1737 . . 1752 1763 + TstI CACNNNNNNTCC 1743 1738 1775 1770 1752 1758 + TspRI CASTGNN 1758 1749 . . 1756 1762 + SapI GCTCTTC 1763 1766 . . 1752 1757 - BtsI GCAGTG 1751 1749 . . 1757 1762 + Ksp632I* CTCTTC 1763 1766 . . 1758 1762 - MboII GAAGA 1750 1749 . . 1762 1766 + Hin4II* CCTTC 1772 1771 . . 1777 1781 + MaeIII GTNAC 1776 1781 . . 1800 1803 + TspEI AATT 1799 1803 . . 1812 1823 + BdaI TGANNNNNNTCA 1801 1799 1835 1833 1814 1817 + SetI ASST 1817 1813 . . 1815 1825 + EcoNI CCTNNNNNAGG 1819 1820 . . 1815 1825 + BsiYI* CCNNNNNNNGG 1821 1818 . . 1823 1826 + SetI ASST 1826 1822 . . 1812 1823 - BdaI TGANNNNNNTCA 1835 1833 1801 1799 1828 1832 + TfiI GAWTC 1828 1831 . . 1828 1832 + HinfI GANTC 1828 1831 . . 1847 1850 + MnlI CCTC 1857 1856 . . 1848 1852 + DdeI CTNAG 1848 1851 . . 1848 1852 + BspCNI CTCAG 1861 1859 . . 1848 1852 + BseMII CTCAG 1862 1860 . . 1849 1853 + Hpy188I TCNGA 1851 1850 . . 1860 1864 + BssKI CCNGG 1859 1864 . . 1860 1863 + HpaII CCGG 1860 1862 . . 1860 1864 + ScrFI CCNGG 1861 1862 . . 1860 1864 + CauII* CCSGG 1861 1862 . . 1871 1874 + MseI TTAA 1871 1873 . . 1881 1885 + FokI GGATG 1894 1898 . . 1881 1885 + BseGI GGATG 1887 1885 . . 1883 1887 + TspDTI ATGAA 1898 1896 . . 1887 1892 + Cfr10I RCCGGY 1887 1891 . . 1887 1892 + BetI* WCCGGW 1887 1891 . . 1887 1892 + AgeI ACCGGT 1887 1891 . . 1888 1891 + HpaII CCGG 1888 1890 . . 1895 1900 + StyI CCWWGG 1895 1899 . . 1895 1900 + SecI* CCNNGG 1895 1899 . . 1899 1902 + CviJI RGCY 1900 1900 . . 1908 1918 + FalI AAGNNNNNCTT 1899 1894 1931 1926 1908 1918 - FalI AAGNNNNNCTT 1931 1926 1899 1894 1921 1924 + MseI TTAA 1921 1923 . . 1923 1928 + ApoI RAATTY 1923 1927 . . 1924 1927 + TspEI AATT 1923 1927 . . 1937 1940 + TaiI ACGT 1940 1936 . . 1937 1940 + SetI ASST 1940 1936 . . 1937 1940 + MaeII ACGT 1937 1939 . . 1939 1942 + Csp6I GTAC 1939 1941 . . 1939 1942 + RsaI GTAC 1940 1940 . . 1942 1947 + SmlI CTYRAG 1942 1946 . . 1947 1950 + CviJI RGCY 1948 1948 . . 1942 1947 - Bce83I* CTTGAG 1927 1925 . . 1947 1952 + NlaIV GGNNCC 1949 1949 . . 1954 1958 - TspDTI ATGAA 1944 1942 . . 1958 1961 + MseI TTAA 1958 1960 . . 1968 1978 + FalI AAGNNNNNCTT 1959 1954 1991 1986 1974 1977 + SetI ASST 1977 1973 . . 1968 1978 - FalI AAGNNNNNCTT 1991 1986 1959 1954 1982 1985 + MaeI CTAG 1982 1984 . . 1987 1997 + Hin4I GAYNNNNNVTC 1978 1973 2010 2005 1987 1991 - SfaNI GCATC 1977 1981 . . 1987 1997 - Hin4I GAYNNNNNVTC 2010 2005 1978 1973 2007 2010 + CviJI RGCY 2008 2008 . . 2009 2020 + BstXI CCANNNNNNTGG 2016 2012 . . 2011 2014 + TspEI AATT 2010 2014 . . 2034 2040 + TspRI CASTGNN 2040 2031 . . 2042 2045 + CviJI RGCY 2043 2043 . . 2059 2069 + Hin4I GAYNNNNNVTC 2050 2045 2082 2077 2063 2066 + SetI ASST 2066 2062 . . 2067 2071 + BsmAI GTCTC 2072 2076 . . 2059 2069 - Hin4I GAYNNNNNVTC 2082 2077 2050 2045 2075 2079 + DdeI CTNAG 2075 2078 . . 2075 2079 + BspCNI CTCAG 2088 2086 . . 2075 2079 + BseMII CTCAG 2089 2087 . . 2078 2081 + CviJI RGCY 2079 2079 . . 2080 2084 - BsrI ACTGG 2080 2078 . . 2088 2091 + CviRI* TGCA 2089 2089 . . 2098 2101 + FatI CATG 2097 2101 . . 2098 2101 + NlaIII CATG 2101 2097 . . 2098 2101 + CviAII CATG 2098 2100 . . 2113 2116 + TspEI AATT 2112 2116 . . 2119 2123 - BsrI ACTGG 2119 2117 . . 2136 2139 + SetI ASST 2139 2135 . . 2137 2141 + Hin4II* CCTTC 2147 2146 . . 2145 2154 + XmnI GAANNNNTTC 2149 2149 . . 2169 2173 + FokI GGATG 2182 2186 . . 2169 2173 + BseGI GGATG 2175 2173 . . 2167 2170 - MnlI CCTC 2160 2159 . . 2178 2181 + TspEI AATT 2177 2181 . . 2190 2194 + MboII GAAGA 2202 2201 . . 2192 2197 + XhoII RGATCY 2192 2196 . . 2192 2197 + BglII AGATCT 2192 2196 . . 2193 2196 + DpnI GATC 2194 2194 . . 2193 2196 + BstKTI GATC 2195 2193 . . 2193 2196 + MboI GATC 2192 2196 . . 2199 2204 + DsaI* CCRYGG 2199 2203 . . 2199 2204 + SecI* CCNNGG 2199 2203 . . 2198 2202 - BceAI ACGGC 2183 2185 . . 2209 2213 + TspDTI ATGAA 2224 2222 . . 2217 2227 + Hin4I GAYNNNNNVTC 2208 2203 2240 2235 2229 2234 + SmaI CCCGGG 2231 2231 . . 2229 2234 + Cfr9I CCCGGG 2229 2233 . . 2229 2233 + BssKI CCNGG 2228 2233 . . 2229 2234 + SecI* CCNNGG 2229 2233 . . 2229 2234 + BmeT110I CYCGRG 2230 2232 . . 2229 2233 + ScrFI CCNGG 2230 2231 . . 2229 2233 + CauII* CCSGG 2230 2231 . . 2229 2234 + AvaI CYCGRG 2229 2233 . . 2230 2234 + BssKI CCNGG 2229 2234 . . 2230 2233 + HpaII CCGG 2230 2232 . . 2230 2234 + ScrFI CCNGG 2231 2232 . . 2226 2230 - HphI GGTGA 2218 2217 . . 2230 2234 + CauII* CCSGG 2231 2232 . . 2243 2246 + SetI ASST 2246 2242 . . 2243 2246 + CviJI RGCY 2244 2244 . . 2243 2246 + AluI AGCT 2244 2244 . . 2248 2253 + Hpy166II GTNNAC 2250 2250 . . 2248 2253 + BssNAI GTATAC 2250 2250 . . 2248 2253 + AccI GTMKAC 2249 2251 . . 2251 2256 + BsaAI YACGTR 2253 2253 . . 2252 2255 + TaiI ACGT 2255 2251 . . 2252 2255 + SetI ASST 2255 2251 . . 2252 2255 + MaeII ACGT 2252 2254 . . 2252 2257 + AflIII ACRYGT 2252 2256 . . 2257 2262 + MmeI TCCRAC 2282 2280 . . 2257 2261 + Hpy188I TCNGA 2259 2258 . . 2260 2264 + HinfI GANTC 2260 2263 . . 2262 2266 + DdeI CTNAG 2262 2265 . . 2262 2266 + BspCNI CTCAG 2275 2273 . . 2262 2266 + BseMII CTCAG 2276 2274 . . 2263 2267 + Hpy188I TCNGA 2265 2264 . . 2260 2264 - PleI GAGTC 2254 2255 . . 2260 2264 - MlyI GAGTC 2254 2254 . . 2269 2273 + TfiI GAWTC 2269 2272 . . 2269 2273 + HinfI GANTC 2269 2272 . . 2272 2276 - MboII GAAGA 2264 2263 . . 2278 2282 + Hpy188I TCNGA 2280 2279 . . 2275 2279 - MboII GAAGA 2267 2266 . . 2276 2281 - Eco57MI CTGRAG 2261 2259 . . 2276 2281 - Eco57I CTGAAG 2261 2259 . . 2290 2293 + CviJI RGCY 2291 2291 . . 2311 2314 + MseI TTAA 2311 2313 . . 2314 2317 + SetI ASST 2317 2313 . . 2314 2317 + CviJI RGCY 2315 2315 . . 2314 2317 + AluI AGCT 2315 2315 . . 2320 2324 + HphI GGTGA 2332 2331 . . 2323 2327 + TfiI GAWTC 2323 2326 . . 2323 2327 + HinfI GANTC 2323 2326 . . 2330 2334 + TspDTI ATGAA 2345 2343 . . 2341 2345 + MaeIII GTNAC 2340 2345 . . 2373 2377 + BmgT120I GGNCC 2374 2375 . . 2373 2377 + AvaII GGWCC 2373 2376 . . 2373 2377 + AsuI* GGNCC 2373 2376 . . 2376 2386 + BsiYI* CCNNNNNNNGG 2382 2379 . . 2378 2383 + ApoI RAATTY 2378 2382 . . 2379 2382 + TspEI AATT 2378 2382 . . 2386 2391 + Cac8I GCNNGC 2388 2388 . . 2401 2404 + DpnI GATC 2402 2402 . . 2401 2404 + BstKTI GATC 2403 2401 . . 2401 2404 + MboI GATC 2400 2404 . . 2401 2405 - BinI* GGATC 2395 2396 . . 2425 2429 + Hpy188I TCNGA 2427 2426 . . 2428 2431 + DpnI GATC 2429 2429 . . 2428 2431 + BstKTI GATC 2430 2428 . . 2428 2431 + MboI GATC 2427 2431 . . 2430 2435 + Hpy178III* TCNNGA 2431 2433 . . 2435 2438 + TspEI AATT 2434 2438 . . 2457 2460 - MnlI CCTC 2450 2449 . . 2463 2466 + SetI ASST 2466 2462 . . 2463 2466 + CviJI RGCY 2464 2464 . . 2463 2466 + AluI AGCT 2464 2464 . . 2501 2504 + SetI ASST 2504 2500 . . 2509 2521 + AloI GAACNNNNNNTCC 2501 2496 2533 2528 2516 2521 + XhoII RGATCY 2516 2520 . . 2516 2521 + NlaIV GGNNCC 2518 2518 . . 2516 2521 + BamHI GGATCC 2516 2520 . . 2516 2520 + BinI* GGATC 2524 2525 . . 2517 2520 + DpnI GATC 2518 2518 . . 2517 2520 + BstKTI GATC 2519 2517 . . 2517 2520 + MboI GATC 2516 2520 . . 2517 2521 - BinI* GGATC 2511 2512 . . 2536 2539 + MseI TTAA 2536 2538 . . 2551 2554 + MseI TTAA 2551 2553 . . 2554 2559 + SpeI ACTAGT 2554 2558 . . 2555 2558 + MaeI CTAG 2555 2557 . . 2560 2564 + Tsp45I GTSAC 2559 2564 . . 2560 2564 + MaeIII GTNAC 2559 2564 . . 2569 2579 + Hin4I GAYNNNNNVTC 2560 2555 2592 2587 2591 2595 + TspDTI ATGAA 2606 2604 . . 2623 2628 + Hpy166II GTNNAC 2625 2625 . . 2623 2628 + SduI GDGCHC 2627 2623 . . 2623 2628 + BseSI GKGCMC 2627 2623 . . 2623 2628 + ApaLI GTGCAC 2623 2627 . . 2623 2628 + HgiAI* GWGCWC 2627 2623 . . 2624 2627 + CviRI* TGCA 2625 2625 . . 2631 2635 - Hin4II* CCTTC 2625 2624 . . 2645 2650 + SspI AATATT 2647 2647 . . 2661 2664 + CviRI* TGCA 2662 2662 . . 2691 2697 + TspRI CASTGNN 2697 2688 . . 2707 2711 + Tsp45I GTSAC 2706 2711 . . 2707 2711 + MaeIII GTNAC 2706 2711 . . 2739 2744 + TsoI TARCCA 2755 2753 . . 2740 2743 + CviJI RGCY 2741 2741 . . 2751 2756 + ApoI RAATTY 2751 2755 . . 2752 2755 + TspEI AATT 2751 2755 . . 2755 2759 - MboII GAAGA 2747 2746 . . 2758 2762 - MboII GAAGA 2750 2749 . . 2762 2765 + AciI CCGC 2762 2764 . . 2770 2774 + BccI CCATC 2778 2779 . . 2771 2775 - FokI GGATG 2757 2761 . . 2771 2775 - BseGI GGATG 2770 2768 . . 2777 2782 + ApoI RAATTY 2777 2781 . . 2778 2781 + TspEI AATT 2777 2781 . . 2781 2786 + MmeI TCCRAC 2806 2804 . . 2785 2789 + Tsp4CI* ACNGT 2787 2786 . . 2791 2795 - MboII GAAGA 2783 2782 . . 2790 2795 - BbvII* GAAGAC 2783 2787 . . 2782 2795 - AjuI GAANNNNNNNTTGG 2807 2802 2775 2770 2804 2808 + TfiI GAWTC 2804 2807 . . 2804 2808 + HinfI GANTC 2804 2807 . . 2806 2811 + AsuII TTCGAA 2807 2809 . . 2807 2810 + TaqI TCGA 2807 2809 . . 2809 2813 + MboII GAAGA 2821 2820 . . 2819 2822 + SetI ASST 2822 2818 . . 2819 2822 + CviJI RGCY 2820 2820 . . 2819 2822 + AluI AGCT 2820 2820 . . 2821 2825 + DdeI CTNAG 2821 2824 . . 2825 2828 + Csp6I GTAC 2825 2827 . . 2825 2828 + RsaI GTAC 2826 2826 . . 2849 2853 + MboII GAAGA 2861 2860 . . 2849 2854 - Ksp632I* CTCTTC 2844 2847 . . 2872 2875 + TspEI AATT 2871 2875 . . 2876 2879 + MaeI CTAG 2876 2878 . . 2895 2899 - Hin4II* CCTTC 2889 2888 . . 2903 2906 + SetI ASST 2906 2902 . . 2914 2917 + SetI ASST 2917 2913 . . 2914 2917 + CviJI RGCY 2915 2915 . . 2914 2917 + AluI AGCT 2915 2915 . . 2922 2925 - MnlI CCTC 2915 2914 . . 2925 2935 + MwoI GCNNNNNNNGC 2931 2928 . . 2927 2937 + FalI AAGNNNNNCTT 2918 2913 2950 2945 2933 2936 + CviJI RGCY 2934 2934 . . 2934 2939 + Cac8I GCNNGC 2936 2936 . . 2937 2940 + CviRI* TGCA 2938 2938 . . 2927 2937 - FalI AAGNNNNNCTT 2950 2945 2918 2913 2940 2945 + ApoI RAATTY 2940 2944 . . 2941 2944 + TspEI AATT 2940 2944 . . 2956 2960 - TspGWI ACGGA 2946 2944 . . 2980 2984 + Hin4II* CCTTC 2990 2989 . . 2983 2987 + Hpy188I TCNGA 2985 2984 . . 2987 2990 + SetI ASST 2990 2986 . . 2987 2990 + CviJI RGCY 2988 2988 . . 2987 2990 + AluI AGCT 2988 2988 . . 2992 2997 + MmeI TCCRAC 3017 3015 . . 2992 2996 + Hpy188I TCNGA 2994 2993 . . 2996 3000 + Tsp4CI* ACNGT 2998 2997 . . 2997 3003 + TspRI CASTGNN 3003 2994 . . 3012 3015 + TspEI AATT 3011 3015 . . 3014 3017 + MseI TTAA 3014 3016 . . 3026 3029 + CviRI* TGCA 3027 3027 . . 3030 3034 + MaeIII GTNAC 3029 3034 . . 3035 3038 + MseI TTAA 3035 3037 . . 3056 3059 + SetI ASST 3059 3055 . . 3059 3062 + MseI TTAA 3059 3061 . . 3069 3072 + TspEI AATT 3068 3072 . . 3079 3082 + TspEI AATT 3078 3082 . . 3092 3096 + Tsp45I GTSAC 3091 3096 . . 3092 3096 + MaeIII GTNAC 3091 3096 . . 3111 3114 + SetI ASST 3114 3110 . . 3112 3116 + HphI GGTGA 3124 3123 . . 3121 3125 + MboII GAAGA 3133 3132 . . 3139 3143 + Hpy188I TCNGA 3141 3140 . . 3144 3147 + TspEI AATT 3143 3147 . . 3146 3149 + MseI TTAA 3146 3148 . . 3164 3167 + TspEI AATT 3163 3167 . . 3176 3179 + CviJI RGCY 3177 3177 . . 3183 3186 + CviRI* TGCA 3184 3184 . . 3184 3194 + MwoI GCNNNNNNNGC 3190 3187 . . 3181 3185 - SfaNI GCATC 3171 3175 . . 3192 3195 + CviJI RGCY 3193 3193 . . 3194 3204 + BsiYI* CCNNNNNNNGG 3200 3197 . . 3194 3204 + PflMI CCANNNNNTGG 3200 3197 . . 3211 3215 + TspDTI ATGAA 3226 3224 . . 3213 3217 + MboII GAAGA 3225 3224 . . 3213 3218 + BbvII* GAAGAC 3220 3224 . . 3216 3220 + HinfI GANTC 3216 3219 . . 3218 3222 + DdeI CTNAG 3218 3221 . . 3218 3222 + BspCNI CTCAG 3231 3229 . . 3218 3222 + BseMII CTCAG 3232 3230 . . 3219 3223 + Hpy188I TCNGA 3221 3220 . . 3216 3220 - PleI GAGTC 3210 3211 . . 3216 3220 - MlyI GAGTC 3210 3210 . . 3232 3237 + MmeI TCCRAC 3257 3255 . . 3237 3240 + FatI CATG 3236 3240 . . 3237 3240 + NlaIII CATG 3240 3236 . . 3237 3240 + CviAII CATG 3237 3239 . . 3252 3256 + Hpy188I TCNGA 3254 3253 . . 3263 3268 + Hpy166II GTNNAC 3265 3265 . . 3267 3270 + SetI ASST 3270 3266 . . 3282 3287 + BtrI CACGTC 3284 3284 . . 3283 3286 + TaiI ACGT 3286 3282 . . 3283 3286 + SetI ASST 3286 3282 . . 3283 3286 + MaeII ACGT 3283 3285 . . 3284 3288 + Hpy99I CGWCG 3288 3283 . . 3286 3291 - TaqII GACCGA 3276 3274 . . 3297 3302 + BspLU11I* ACATGT 3297 3301 . . 3297 3302 + NspI RCATGY 3301 3297 . . 3297 3302 + AflIII ACRYGT 3297 3301 . . 3298 3301 + FatI CATG 3297 3301 . . 3298 3301 + NlaIII CATG 3301 3297 . . 3298 3301 + CviAII CATG 3298 3300 . . 3305 3308 + TaqI TCGA 3305 3307 . . 3307 3312 + SduI GDGCHC 3311 3307 . . 3307 3312 + HgiJII* GRGCYC 3311 3307 . . 3308 3311 + CviJI RGCY 3309 3309 . . 3310 3315 + BmeT110I CYCGRG 3311 3313 . . 3310 3315 + AvaI CYCGRG 3310 3314 . . 3316 3321 + NdeI CATATG 3317 3319 . . 3325 3329 - SfaNI GCATC 3315 3319 . . 3333 3336 + TspEI AATT 3332 3336 . . 3340 3346 + SauI* CCTNAGG 3341 3344 . . 3341 3345 + DdeI CTNAG 3341 3344 . . 3353 3356 + CviRI* TGCA 3354 3354 . . 3357 3360 + TspEI AATT 3356 3360 . . 3369 3373 + Tsp4CI* ACNGT 3371 3370 . . 3373 3376 + MseI TTAA 3373 3375 . . 3380 3383 + MseI TTAA 3380 3382 . . 3377 3382 - TsoI TARCCA 3367 3365 . . 3392 3395 + CviJI RGCY 3393 3393 . . 3394 3398 + Hin4II* CCTTC 3404 3403 . . 3397 3401 - MboII GAAGA 3389 3388 . . 3403 3406 + AciI CCGC 3403 3405 . . 3402 3407 - EciI GGCGGA 3392 3390 . . 3409 3419 + Hin4I GAYNNNNNVTC 3400 3395 3432 3427 3409 3419 - Hin4I GAYNNNNNVTC 3432 3427 3400 3395 3424 3429 + BsgI GTGCAG 3445 3443 . . 3425 3428 + CviRI* TGCA 3426 3426 . . 3426 3430 + BlsI GCNGC 3428 3427 . . 3426 3430 + BisI GCNGC 3427 3428 . . 3426 3430 + BbvI GCAGC 3438 3442 . . 3426 3430 + TseI GCWGC 3426 3429 . . 3427 3432 + PvuII CAGCTG 3429 3429 . . 3427 3432 + NspBII* CMGCKG 3429 3429 . . 3428 3431 + SetI ASST 3431 3427 . . 3428 3431 + CviJI RGCY 3429 3429 . . 3428 3431 + AluI AGCT 3429 3429 . . 3429 3435 + EspI* GCTNAGC 3430 3433 . . 3430 3434 + DdeI CTNAG 3430 3433 . . 3432 3437 + SduI GDGCHC 3436 3432 . . 3432 3437 + HgiAI* GWGCWC 3436 3432 . . 3430 3434 - BspCNI CTCAG 3422 3420 . . 3430 3434 - BseMII CTCAG 3421 3419 . . 3465 3474 + XmnI GAANNNNTTC 3469 3469 . . 3470 3473 + TspEI AATT 3469 3473 . . 3473 3476 + TaqI TCGA 3473 3475 . . 3476 3480 + Tsp4CI* ACNGT 3478 3477 . . 3485 3488 + MseI TTAA 3485 3487 . . 3487 3490 + TspEI AATT 3486 3490 . . 3510 3515 + SecI* CCNNGG 3510 3514 . . 3511 3515 + BssKI CCNGG 3510 3515 . . 3511 3515 + BseBI CCWGG 3512 3513 . . 3511 3515 + ScrFI CCNGG 3512 3513 . . 3511 3515 + EcoRII CCWGG 3510 3515 . . 3511 3521 + BsiYI* CCNNNNNNNGG 3517 3514 . . 3525 3530 + Cac8I GCNNGC 3527 3527 . . 3529 3533 + SfaNI GCATC 3538 3542 . . 3532 3536 + Hpy188I TCNGA 3534 3533 . . 3555 3560 + SmlI CTYRAG 3555 3559 . . 3556 3561 + Hpy178III* TCNNGA 3557 3559 . . 3555 3560 - Bce83I* CTTGAG 3540 3538 . . 3560 3569 + PshAI GACNNNNGTC 3564 3564 . . 3561 3564 + SetI ASST 3564 3560 . . 3561 3566 + BspMI ACCTGC 3570 3574 . . 3565 3569 - HgaI GACGC 3554 3559 . . 3572 3576 + BceAI ACGGC 3588 3590 . . 3574 3577 + CviJI RGCY 3575 3575 . . 3586 3591 - MmeI TCCRAC 3567 3565 . . 3597 3602 + NlaIV GGNNCC 3599 3599 . . 3589 3601 - AloI GAACNNNNNNTCC 3613 3608 3581 3576 3610 3614 + MboII GAAGA 3622 3621 . . 3613 3616 + DpnI GATC 3614 3614 . . 3613 3616 + BstKTI GATC 3615 3613 . . 3613 3616 + MboI GATC 3612 3616 . . 3616 3620 + Hpy99I CGWCG 3620 3615 . . 3618 3621 + TaqI TCGA 3618 3620 . . 3632 3635 + TaqI TCGA 3632 3634 . . 3633 3637 + Hpy99I CGWCG 3637 3632 . . 3647 3651 + Tsp4CI* ACNGT 3649 3648 . . 3662 3667 + ApoI RAATTY 3662 3666 . . 3663 3666 + TspEI AATT 3662 3666 . . 3667 3670 + MseI TTAA 3667 3669 . . 3670 3675 + SpeI ACTAGT 3670 3674 . . 3671 3674 + MaeI CTAG 3671 3673 . . 3676 3681 + Hpy166II GTNNAC 3678 3678 . . 3676 3681 + HindII GTYRAC 3678 3678 . . 3680 3683 + TaiI ACGT 3683 3679 . . 3680 3683 + SetI ASST 3683 3679 . . 3680 3683 + MaeII ACGT 3680 3682 . . 3689 3698 + XmnI GAANNNNTTC 3693 3693 . . 3703 3707 + BsrI ACTGG 3708 3706 . . 3707 3710 + Csp6I GTAC 3707 3709 . . 3707 3710 + RsaI GTAC 3708 3708 . . 3725 3730 + TatI WGTACW 3725 3729 . . 3725 3730 + Bsp1407I TGTACA 3725 3729 . . 3726 3731 + Hpy166II GTNNAC 3728 3728 . . 3726 3729 + Csp6I GTAC 3726 3728 . . 3726 3729 + RsaI GTAC 3727 3727 . . 3756 3759 + TspEI AATT 3755 3759 . . 3775 3779 + Tsp45I GTSAC 3774 3779 . . 3775 3779 + MaeIII GTNAC 3774 3779 . . 3776 3780 - HphI GGTGA 3768 3767 . . 3786 3791 + AsuII TTCGAA 3787 3789 . . 3787 3790 + TaqI TCGA 3787 3789 . . 3789 3793 - Hin4II* CCTTC 3783 3782 . . 3795 3798 + FatI CATG 3794 3798 . . 3795 3798 + NlaIII CATG 3798 3794 . . 3795 3798 + CviAII CATG 3795 3797 . . 3796 3800 + TspDTI ATGAA 3811 3809 . . 3802 3806 + MboII GAAGA 3814 3813 . . 3803 3813 + FalI AAGNNNNNCTT 3794 3789 3826 3821 3805 3809 - BccI CCATC 3799 3800 . . 3803 3813 - FalI AAGNNNNNCTT 3826 3821 3794 3789 3823 3828 + EcoRV GATATC 3825 3825 . . 3835 3838 + TaqI TCGA 3835 3837 . . 3841 3851 + MwoI GCNNNNNNNGC 3847 3844 . . 3841 3845 + BlsI GCNGC 3843 3842 . . 3841 3845 + BisI GCNGC 3842 3843 . . 3841 3845 + BbvI GCAGC 3853 3857 . . 3841 3845 + TseI GCWGC 3841 3844 . . 3844 3848 + SfaNI GCATC 3853 3857 . . 3849 3854 + HaeII RGCGCY 3853 3849 . . 3850 3853 + Hin6I GCGC 3850 3852 . . 3850 3853 + GlaI GCGC 3851 3851 . . 3850 3853 + HhaI GCGC 3852 3850 . . 3855 3859 + FokI GGATG 3868 3872 . . 3855 3859 + BseGI GGATG 3861 3859 . . 3857 3861 + TspDTI ATGAA 3872 3870 . . 3871 3876 + MfeI CAATTG 3871 3875 . . 3872 3875 + TspEI AATT 3871 3875 . . 3876 3879 + CviJI RGCY 3877 3877 . . 3882 3885 + CviRI* TGCA 3883 3883 . . 3887 3891 + TspDTI ATGAA 3902 3900 . . 3889 3898 + XmnI GAANNNNTTC 3893 3893 . . 3891 3894 + TspEI AATT 3890 3894 . . 3897 3902 - TaqII GACCGA 3887 3885 . . 3907 3911 + Hpy188I TCNGA 3909 3908 . . 3911 3915 + TspDTI ATGAA 3926 3924 . . 3921 3924 - MnlI CCTC 3914 3913 . . 3926 3930 + TspDTI ATGAA 3941 3939 . . 3930 3933 + TspEI AATT 3929 3933 . . 3935 3944 + XmnI GAANNNNTTC 3939 3939 . . 3951 3954 + SetI ASST 3954 3950 . . 3952 3956 + HphI GGTGA 3964 3963 . . 3958 3961 + MseI TTAA 3958 3960 . . 3978 3983 + Hpy178III* TCNNGA 3979 3981 . . 4003 4007 + Hpy188I TCNGA 4005 4004 . . 4012 4016 - SfaNI GCATC 4002 4006 . . 4020 4023 + TspEI AATT 4019 4023 . . 4022 4025 + MseI TTAA 4022 4024 . . 4028 4031 + TspEI AATT 4027 4031 . . 4032 4036 - BccI CCATC 4026 4027 . . 4068 4072 + Tsp4CI* ACNGT 4070 4069 . . #--------------------------------------- # # # AatII: # [GACGTC] # 982 3095 # # AccI: # [GTMKAC] # 1828 2249 # # AciI: # [CCGC] # 173 641 674 1214 1375 # # AcyI: # [GRCGYC] # 154 332 647 2944 # # AflIII: # [ACRYGT] # 780 1045 2252 # # AgeI: # [ACCGGT] # 1887 2190 # # AhaIII*: # [TTTAAA] # 1736 2341 # # AjuI: # [GAANNNNNNNTTGG] # 32 1270 2775 # # AloI: # [GAACNNNNNNTCC] # 32 32 464 1048 2501 # # AluI: # [AGCT] # 71 73 95 149 149 222 # 356 441 533 648 656 684 # # ApaLI: # [GTGCAC] # 1454 2623 # # ApoI: # [RAATTY] # 26 45 77 163 373 415 # 455 702 722 1099 # # AsuI*: # [GGNCC] # 29 306 820 1218 1704 # # AsuII: # [TTCGAA] # 290 980 1090 1717 # # AvaI: # [CYCGRG] # 767 1081 2229 # # AvaII: # [GGWCC] # 29 306 1704 2038 # # BaeI: # [ACNNNNGTAYC] # 33 464 3580 # # BamHI: # [GGATCC] # 1561 2516 # # BbvI: # [GCAGC] # 224 415 447 2991 # # BbvII*: # [GAAGAC] # 118 437 857 1018 1647 # # BccI: # [CCATC] # 51 89 114 227 319 1021 # 1029 1227 # # Bce83I*: # [CTTGAG] # 58 537 743 1126 1613 # # BceAI: # [ACGGC] # 150 489 1405 2033 # # BclI: # [TGATCA] # 1044 3033 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 587 1180 2242 # # BetI*: # [WCCGGW] # 798 1089 2190 # # BglII: # [AGATCT] # 1885 2192 # # BinI*: # [GGATC] # 13 116 639 1553 1756 # # BisI: # [GCNGC] # 235 415 436 951 2040 # # BlsI: # [GCNGC] # 234 415 437 951 2040 # # BmeT110I: # [CYCGRG] # 766 1081 2230 # # BmgT120I: # [GGNCC] # 30 306 820 1218 1703 # # BmtI: # [GCTAGC] # 389 3688 # # BsaAI: # [YACGTR] # 472 837 944 1824 # # BsaBI: # [GATNNNNATC] # 1153 2924 # # BseBI: # [CCWGG] # 565 824 2688 # # BseGI: # [GGATG] # 216 247 288 486 595 1091 # 1154 # # BseMII: # [CTCAG] # 187 189 227 656 956 1862 # # BseRI: # [GAGGAG] # 817 3260 # # BseSI: # [GKGCMC] # 1450 2627 # # BseYI: # [CCCAGC] # 1556 2521 # # BsgI: # [GTGCAG] # 632 1048 2397 # # BsiYI*: # [CCNNNNNNNGG] # 42 218 317 332 560 561 # 818 1229 # # BslFI: # [GGGAC] # 990 3087 # # BsmAI: # [GTCTC] # 398 1674 2005 # # BsmI: # [GAATGC] # 846 3231 # # Bsp1407I: # [TGTACA] # 352 3725 # # BspCNI: # [CTCAG] # 187 191 227 655 956 1861 # # BspHI: # [TCATGA] # 1397 2680 # # BspLU11I*: # [ACATGT] # 780 3297 # # BspMI: # [ACCTGC] # 380 507 3190 # # BsrI: # [ACTGG] # 39 58 369 858 1164 1589 # # BssKI: # [CCNGG] # 1 369 398 424 567 1037 # 1281 # # BssNAI: # [GTATAC] # 1827 2250 # # BstKTI: # [GATC] # 27 31 89 208 382 462 # 634 1096 1148 # # BstXI: # [CCANNNNNNTGG] # 2016 2061 # # BtrI: # [CACGTC] # 793 3284 # # BtsI: # [GCAGTG] # 691 1060 2326 # # Cac8I: # [GCNNGC] # 44 387 548 550 591 1957 # # CauII*: # [CCSGG] # 1 369 426 1435 1846 # # Cfr10I: # [RCCGGY] # 778 1109 2190 # # Cfr9I: # [CCCGGG] # 1848 2229 # # Csp6I: # [GTAC] # 4 8 19 33 139 308 # 351 466 469 512 882 886 # # CviAII: # [CATG] # 51 61 282 379 497 700 # 968 1139 # # CviJI: # [RGCY] # 6 15 16 19 24 24 # 25 28 35 36 36 36 # 47 48 52 54 60 63 # 63 68 70 75 79 84 # 88 95 106 116 116 143 # 146 149 165 165 189 189 # 200 225 277 302 343 # # CviRI*: # [TGCA] # 19 37 72 89 108 125 # 157 170 194 234 261 276 # 341 403 457 536 598 # # DdeI: # [CTNAG] # 51 89 123 136 187 227 # 397 425 538 559 647 698 # # DinI: # [GGCGCC] # 648 3429 # # DpnI: # [GATC] # 27 31 89 208 382 463 # 633 1096 1148 # # DraII: # [RGGNCCY] # 1553 2524 # # DsaI*: # [CCRYGG] # 1878 2199 # # EciI: # [GGCGGA] # 685 3392 # # Eco31I: # [GGTCTC] # 398 3679 # # Eco57I: # [CTGAAG] # 834 1427 1816 # # Eco57MI: # [CTGRAG] # 834 1427 1816 # # EcoNI: # [CCTNNNNNAGG] # 218 1601 2258 # # EcoP15I: # [CAGCAG] # 288 377 3412 # # EcoRII: # [CCWGG] # 567 822 2688 # # EcoRV: # [GATATC] # 252 488 3337 # # EspI*: # [GCTNAGC] # 647 3430 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 28 32 32 32 # 32 251 844 927 1899 # # FatI: # [CATG] # 51 61 283 378 497 700 # 968 1139 # # FokI: # [GGATG] # 209 267 288 466 575 1111 # 1161 # # GlaI: # [GCGC] # 214 226 434 3203 # # GsaI: # [CCCAGC] # 1560 2517 # # HaeII: # [RGCGCY] # 224 650 3203 # # HaeIII: # [GGCC] # 106 165 168 319 796 2523 # # HgaI: # [GACGC] # 523 1141 2413 # # HgiAI*: # [GWGCWC] # 641 809 1222 1405 # # HgiCI*: # [GGYRCC] # 646 3431 # # HgiJII*: # [GRGCYC] # 766 3311 # # HhaI: # [GCGC] # 214 225 435 3203 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 32 32 32 32 # 32 32 32 40 126 207 # 320 645 742 808 965 # # Hin4II*: # [CCTTC] # 22 46 79 101 151 175 # 264 294 300 327 335 337 # 375 379 414 478 # # Hin6I: # [GCGC] # 214 227 433 3203 # # HindII: # [GTYRAC] # 170 399 3508 # # HindIII: # [AAGCTT] # 531 3546 # # HinfI: # [GANTC] # 9 12 54 62 121 126 # 132 178 212 361 412 432 # 481 624 861 # # HpaII: # [CCGG] # 20 28 342 353 426 1061 # 1847 # # HphI: # [GGTGA] # 5 63 113 114 179 196 # 215 364 546 644 792 846 # # Hpy166II: # [GTNNAC] # 40 47 50 123 349 375 # 413 640 791 1249 # # Hpy178III*: # [TCNNGA] # 82 91 95 98 119 141 # 163 164 213 223 226 422 # 914 1126 # # Hpy188I: # [TCNGA] # 6 9 15 17 33 72 # 80 96 147 147 280 346 # 375 408 558 738 750 # # Hpy99I: # [CGWCG] # 17 332 440 3288 # # KasI: # [GGCGCC] # 646 3431 # # Ksp632I*: # [CTCTTC] # 539 1081 1224 1233 # # MaeI: # [CTAG] # 11 180 321 329 386 406 # 421 573 655 795 # # MaeII: # [ACGT] # 128 136 207 315 397 397 # 436 508 522 1031 # # MaeIII: # [GTNAC] # 60 62 111 147 219 240 # 262 303 323 382 564 683 # 721 # # MboI: # [GATC] # 27 31 89 208 382 465 # 631 1096 1148 # # MboII: # [GAAGA] # 3 3 12 20 33 38 # 40 43 62 92 103 111 # 118 156 164 191 192 192 # 199 233 263 272 299 306 # 452 480 # # McrI*: # [CGRYCG] # 953 3124 # # MfeI: # [CAATTG] # 206 3871 # # MlyI: # [GAGTC] # 867 956 2254 # # MmeI: # [TCCRAC] # 211 240 310 510 524 2282 # # MnlI: # [CCTC] # 5 11 28 30 35 58 # 72 135 156 157 163 168 # 168 195 272 290 303 367 # 465 999 # # MseI: # [TTAA] # 7 15 21 24 37 50 # 55 64 87 105 136 182 # 225 227 291 353 463 536 # 576 623 # # MstI*: # [TGCGCA] # 434 3643 # # MwoI: # [GCNNNNNNNGC] # 230 259 435 657 2496 # # NarI: # [GGCGCC] # 647 3430 # # NdeI: # [CATATG] # 760 3317 # # NheI: # [GCTAGC] # 385 3692 # # NlaIII: # [CATG] # 51 61 279 382 497 700 # 968 1139 # # NlaIV: # [GGNNCC] # 394 478 569 648 907 1081 # # NspBII*: # [CMGCKG] # 648 1189 2240 # # NspI: # [RCATGY] # 433 776 2868 # # PflMI: # [CCANNNNNTGG] # 877 3200 # # PfoI: # [TCCNGGA] # 822 3255 # # PleI: # [GAGTC] # 867 956 2254 # # PshAI: # [GACNNNNGTC] # 513 3564 # # PvuII: # [CAGCTG] # 648 1189 2240 # # RsaI: # [GTAC] # 4 8 19 33 139 308 # 350 466 470 512 882 886 # # SapI: # [GCTCTTC] # 1763 2314 # # SauI*: # [CCTNAGG] # 736 1661 1680 # # ScrFI: # [CCNGG] # 1 369 398 426 565 1037 # 1281 # # SduI: # [GDGCHC] # 125 641 684 1222 1405 # # SecI*: # [CCNNGG] # 30 304 567 668 1227 1281 # # SetI: # [ASST] # 5 5 9 9 9 11 # 16 19 20 21 28 31 # 37 38 41 48 48 53 # 55 60 61 62 69 73 # 73 84 89 89 107 107 # 114 119 123 133 144 145 # 149 154 156 197 209 213 # 255 271 318 # # SfaNI: # [GCATC] # 75 140 144 149 223 315 # 372 592 873 1194 # # SmaI: # [CCCGGG] # 1846 2231 # # SmlI: # [CTYRAG] # 58 522 743 1141 1613 # # SnaBI: # [TACGTA] # 472 3605 # # SpeI: # [ACTAGT] # 407 1116 2554 # # SphI: # [GCATGC] # 433 3644 # # SspI: # [AATATT] # 1430 2647 # # StyI: # [CCWWGG] # 668 1227 2182 # # TaiI: # [ACGT] # 128 139 207 315 394 397 # 436 508 522 1031 # # TaqI: # [TCGA] # 14 43 48 87 131 145 # 155 165 168 242 498 1090 # 1291 # # TaqII: # [CACCCA] # 36 190 287 611 2953 # # TatI: # [WGTACW] # 352 1491 2234 # # TauI: # [GCSGC] # 1389 2688 # # TfiI: # [GAWTC] # 12 54 62 121 126 132 # 178 212 361 441 481 624 # 1273 # # TseI: # [GCWGC] # 236 415 435 2991 # # TsoI: # [TARCCA] # 612 710 2755 # # Tsp45I: # [GTSAC] # 147 303 382 385 683 781 # 1396 # # Tsp4CI*: # [ACNGT] # 7 91 107 164 171 211 # 230 373 421 466 679 1157 # # TspDTI: # [ATGAA] # 15 19 24 30 36 46 # 50 59 61 115 119 121 # 136 249 261 280 282 585 # 620 969 # # TspEI: # [AATT] # 8 10 16 17 19 20 # 24 26 39 45 50 54 # 56 57 65 65 69 71 # 77 83 87 90 93 94 # 95 97 100 102 113 116 # 124 132 152 169 176 201 # 278 299 317 371 # # TspGWI: # [ACGGA] # 1131 2946 # # TspRI: # [CASTGNN] # 260 275 282 306 343 348 # 532 657 1074 # # TstI: # [CACNNNNNNTCC] # 32 1743 2302 # # XhoII: # [RGATCY] # 324 1561 2192 # # XmnI: # [GAANNNNTTC] # 46 138 200 224 925 1224 # 1320 # # ZraI: # [GACGTC] # 980 3097 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4077 # Reported_sequences: 1 # Reported_hitcount: 813 #---------------------------------------