Restriction Fragments for CSN9/YDR179C



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The currently selected gene/sequence is :

CSN9/YDR179C on chromosome IV from coordinates 819196 to 818708.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.32578.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 12 - MnlI CCTC 2 1 . . 13 17 + MboII GAAGA 25 24 . . 31 34 + MaeI CTAG 31 33 . . 34 38 + MboII GAAGA 46 45 . . 36 41 + XhoII RGATCY 36 40 . . 37 40 + DpnI GATC 38 38 . . 37 40 + BstKTI GATC 39 37 . . 37 40 + MboI GATC 36 40 . . 37 41 - BinI* GGATC 31 32 . . 61 65 + MboII GAAGA 73 72 . . 70 73 + MseI TTAA 70 72 . . 67 72 - TsoI TARCCA 57 55 . . 81 86 + XhoII RGATCY 81 85 . . 82 85 + DpnI GATC 83 83 . . 82 85 + BstKTI GATC 84 82 . . 82 85 + MboI GATC 81 85 . . 84 89 + Hpy178III* TCNNGA 85 87 . . 82 86 - BinI* GGATC 76 77 . . 89 93 + TspDTI ATGAA 104 102 . . 98 101 + TspEI AATT 97 101 . . 103 108 + AsuII TTCGAA 104 106 . . 104 107 + TaqI TCGA 104 106 . . 105 116 + BcgI CGANNNNNNTGC 94 92 128 126 108 113 + SspI AATATT 110 110 . . 128 131 + MseI TTAA 128 130 . . 132 137 + XhoII RGATCY 132 136 . . 132 137 + BglII AGATCT 132 136 . . 133 136 + DpnI GATC 134 134 . . 133 136 + BstKTI GATC 135 133 . . 133 136 + MboI GATC 132 136 . . 138 143 + BetI* WCCGGW 138 142 . . 139 142 + HpaII CCGG 139 141 . . 162 167 + VspI ATTAAT 163 165 . . 163 166 + MseI TTAA 163 165 . . 170 173 + DpnI GATC 171 171 . . 170 173 + BstKTI GATC 172 170 . . 170 173 + MboI GATC 169 173 . . 177 181 + BsrI ACTGG 182 180 . . 178 183 + Eco57MI CTGRAG 199 197 . . 178 183 + GsuI CTGGAG 199 197 . . 183 196 + AjuI GAANNNNNNNTTGG 175 170 207 202 185 188 + TspEI AATT 184 188 . . 187 190 + MseI TTAA 187 189 . . 196 200 + MaeIII GTNAC 195 200 . . 202 205 + MseI TTAA 202 204 . . 223 226 + MseI TTAA 223 225 . . 227 231 + MaeIII GTNAC 226 231 . . 235 242 + PacI TTAATTAA 239 237 . . 235 238 + MseI TTAA 235 237 . . 237 240 + TspEI AATT 236 240 . . 239 242 + MseI TTAA 239 241 . . 244 248 + MboII GAAGA 256 255 . . 255 258 + TspEI AATT 254 258 . . 259 263 + MboII GAAGA 271 270 . . 259 264 + BbvII* GAAGAC 266 270 . . 263 273 + BaeI ACNNNNGTAYC 252 247 285 280 265 269 - BccI CCATC 259 260 . . 275 278 + TaqI TCGA 275 277 . . 280 283 + CviJI RGCY 281 281 . . 286 289 + MseI TTAA 286 288 . . 288 291 + TspEI AATT 287 291 . . 293 296 + SetI ASST 296 292 . . 293 296 + CviJI RGCY 294 294 . . 293 296 + AluI AGCT 294 294 . . 295 300 + SfeI* CTRYAG 295 299 . . 301 306 + SspI AATATT 303 303 . . 307 312 + AhaIII* TTTAAA 309 309 . . 308 311 + MseI TTAA 308 310 . . 322 326 + TfiI GAWTC 322 325 . . 322 326 + HinfI GANTC 322 325 . . 318 322 - BccI CCATC 312 313 . . 340 344 + TspDTI ATGAA 355 353 . . 343 348 + ApoI RAATTY 343 347 . . 344 347 + TspEI AATT 343 347 . . 349 354 + Hpy178III* TCNNGA 350 352 . . 353 357 + MboII GAAGA 365 364 . . 362 366 + Tsp4CI* ACNGT 364 363 . . 363 368 + SfeI* CTRYAG 363 367 . . 373 378 + Hpy166II GTNNAC 375 375 . . 374 379 + TatI WGTACW 374 378 . . 375 378 + Csp6I GTAC 375 377 . . 375 378 + RsaI GTAC 376 376 . . 377 381 + Tsp4CI* ACNGT 379 378 . . 381 386 + Hpy178III* TCNNGA 382 384 . . 381 386 + BspHI TCATGA 381 385 . . 382 385 + FatI CATG 381 385 . . 382 385 + NlaIII CATG 385 381 . . 382 385 + CviAII CATG 382 384 . . 383 387 + TspDTI ATGAA 398 396 . . 402 405 + TspEI AATT 401 405 . . 404 407 + MseI TTAA 404 406 . . 412 415 + AciI CCGC 412 414 . . 413 416 + FnuDII* CGCG 414 414 . . 418 422 + Tsp45I GTSAC 417 422 . . 418 422 + MaeIII GTNAC 417 422 . . 447 451 + Hpy188I TCNGA 449 448 . . 444 448 - MboII GAAGA 436 435 . . 451 454 + SetI ASST 454 450 . . 451 454 + CviJI RGCY 452 452 . . 451 454 + AluI AGCT 452 452 . . 452 457 - GsaI CCCAGC 456 452 . . 452 457 - BseYI CCCAGC 452 456 . . 464 467 + SetI ASST 467 463 . . 464 467 + CviJI RGCY 465 465 . . 464 467 + AluI AGCT 465 465 . . #--------------------------------------- # # # AciI: # [CCGC] # 77 412 # # AhaIII*: # [TTTAAA] # 180 309 # # AjuI: # [GAANNNNNNNTTGG] # 32 175 282 # # AluI: # [AGCT] # 13 24 158 294 # # ApoI: # [RAATTY] # 146 343 # # AsuII: # [TTCGAA] # 104 385 # # BaeI: # [ACNNNNGTAYC] # 33 204 252 # # BbvII*: # [GAAGAC] # 223 266 # # BccI: # [CCATC] # 53 177 259 # # BcgI: # [CGANNNNNNTGC] # 34 94 361 # # BetI*: # [WCCGGW] # 138 351 # # BglII: # [AGATCT] # 132 357 # # BinI*: # [GGATC] # 31 45 413 # # BseYI: # [CCCAGC] # 37 452 # # BspHI: # [TCATGA] # 108 381 # # BsrI: # [ACTGG] # 182 307 # # BstKTI: # [GATC] # 37 39 45 51 317 # # Csp6I: # [GTAC] # 114 375 # # CviAII: # [CATG] # 107 382 # # CviJI: # [RGCY] # 13 13 24 158 281 # # DpnI: # [GATC] # 37 38 45 51 318 # # Eco57MI: # [CTGRAG] # 199 290 # # FatI: # [CATG] # 108 381 # # FnuDII*: # [CGCG] # 75 414 # # GsaI: # [CCCAGC] # 33 456 # # GsuI: # [CTGGAG] # 199 290 # # HinfI: # [GANTC] # 167 322 # # HpaII: # [CCGG] # 139 350 # # Hpy166II: # [GTNNAC] # 114 375 # # Hpy178III*: # [TCNNGA] # 32 85 107 265 # # Hpy188I: # [TCNGA] # 40 449 # # MaeI: # [CTAG] # 31 458 # # MaeIII: # [GTNAC] # 31 72 191 195 # # MboI: # [GATC] # 36 37 45 51 320 # # MboII: # [GAAGA] # 15 21 25 27 53 71 # 94 183 # # MnlI: # [CCTC] # 2 487 # # MseI: # [TTAA] # 4 12 15 21 22 24 # 35 47 58 70 85 96 # # NlaIII: # [CATG] # 104 385 # # PacI: # [TTAATTAA] # 239 250 # # RsaI: # [GTAC] # 113 376 # # SetI: # [ASST] # 13 22 158 296 # # SfeI*: # [CTRYAG] # 68 126 295 # # SspI: # [AATATT] # 110 186 193 # # TaqI: # [TCGA] # 104 171 214 # # TatI: # [WGTACW] # 115 374 # # TfiI: # [GAWTC] # 167 322 # # TsoI: # [TARCCA] # 57 432 # # Tsp45I: # [GTSAC] # 72 417 # # Tsp4CI*: # [ACNGT] # 15 110 364 # # TspDTI: # [ATGAA] # 43 91 104 251 # # TspEI: # [AATT] # 18 33 52 56 58 87 # 88 97 # # VspI: # [ATTAAT] # 163 326 # # XhoII: # [RGATCY] # 36 45 51 357 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 489 # Reported_sequences: 1 # Reported_hitcount: 105 #---------------------------------------