Restriction Fragments for SUP35/YDR172W



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The currently selected gene/sequence is :

SUP35/YDR172W on chromosome IV from coordinates 808324 to 810381.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.21351.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + Hpy188I TCNGA 6 5 . . 7 11 + TfiI GAWTC 7 10 . . 7 11 + HinfI GANTC 7 10 . . 15 20 + StyI CCWWGG 15 19 . . 15 20 + SecI* CCNNGG 15 19 . . 49 52 + CviJI RGCY 50 50 . . 56 60 + Tsp4CI* ACNGT 58 57 . . 58 64 + BstEII GGTNACC 58 63 . . 59 63 + MaeIII GTNAC 58 63 . . 60 65 + TsoI TARCCA 76 74 . . 72 75 + SetI ASST 75 71 . . 74 78 + MaeIII GTNAC 73 78 . . 87 92 + StyI CCWWGG 87 91 . . 87 92 + SecI* CCNNGG 87 91 . . 90 93 + SetI ASST 93 89 . . 83 93 - BaeI ACNNNNGTAYC 108 103 75 70 98 103 + HindIII AAGCTT 98 102 . . 99 102 + SetI ASST 102 98 . . 99 102 + CviJI RGCY 100 100 . . 99 102 + AluI AGCT 100 100 . . 100 110 + MwoI GCNNNNNNNGC 106 103 . . 110 115 + SmlI CTYRAG 110 114 . . 114 117 + CviJI RGCY 115 115 . . 115 125 + MwoI GCNNNNNNNGC 121 118 . . 110 115 - Bce83I* CTTGAG 95 93 . . 117 131 + XcmI CCANNNNNNNNNTGG 124 123 . . 120 123 + SetI ASST 123 119 . . 120 125 + BspMI ACCTGC 129 133 . . 121 128 + Sse8387I CCTGCAGG 126 122 . . 121 131 + BsiYI* CCNNNNNNNGG 127 124 . . 122 127 + PstI CTGCAG 126 122 . . 122 127 + SfeI* CTRYAG 122 126 . . 123 126 + CviRI* TGCA 124 124 . . 126 129 + SetI ASST 129 125 . . 124 129 - BspMI ACCTGC 115 119 . . 124 130 - AarI CACCTGC 115 119 . . 132 135 + Csp6I GTAC 132 134 . . 132 135 + RsaI GTAC 133 133 . . 142 145 + TspEI AATT 141 145 . . 147 161 + XcmI CCANNNNNNNNNTGG 154 153 . . 147 152 + StyI CCWWGG 147 151 . . 147 152 + SecI* CCNNGG 147 151 . . 150 153 + SetI ASST 153 149 . . 161 166 + KpnI GGTACC 165 161 . . 161 166 + NlaIV GGNNCC 163 163 . . 161 166 + HgiCI* GGYRCC 161 165 . . 161 166 + Acc65I GGTACC 161 165 . . 162 165 + Csp6I GTAC 162 164 . . 162 165 + RsaI GTAC 163 163 . . 165 176 + BstXI CCANNNNNNTGG 172 168 . . 171 174 + SetI ASST 174 170 . . 175 178 + CviJI RGCY 176 176 . . 174 179 - TsoI TARCCA 164 162 . . 183 187 + Tsp4CI* ACNGT 185 184 . . 185 190 + TatI WGTACW 185 189 . . 186 189 + Csp6I GTAC 186 188 . . 186 189 + RsaI GTAC 187 187 . . 192 197 + Hpy178III* TCNNGA 193 195 . . 193 203 + BsiYI* CCNNNNNNNGG 199 196 . . 195 199 + Hpy99I CGWCG 199 194 . . 196 200 + HgaI GACGC 205 210 . . 196 201 + AcyI GRCGYC 197 199 . . 199 204 + Cfr10I RCCGGY 199 203 . . 200 203 + HpaII CCGG 200 202 . . 202 208 + BstEII GGTNACC 202 207 . . 203 207 + MaeIII GTNAC 202 207 . . 213 217 + Tsp4CI* ACNGT 215 214 . . 223 226 + MnlI CCTC 233 232 . . 223 233 + EcoNI CCTNNNNNAGG 227 228 . . 223 233 + BsiYI* CCNNNNNNNGG 229 226 . . 224 229 + SmlI CTYRAG 224 228 . . 224 229 - Bce83I* CTTGAG 209 207 . . 232 235 + CviJI RGCY 233 233 . . 230 233 - MnlI CCTC 223 222 . . 240 244 + Tsp4CI* ACNGT 242 241 . . 242 247 + TatI WGTACW 242 246 . . 243 246 + Csp6I GTAC 243 245 . . 243 246 + RsaI GTAC 244 244 . . 250 253 + MnlI CCTC 260 259 . . 250 260 + BsiYI* CCNNNNNNNGG 256 253 . . 251 256 + SmlI CTYRAG 251 255 . . 251 256 - Bce83I* CTTGAG 236 234 . . 257 260 - AciI CCGC 257 259 . . 265 270 + EcoP15I CAGCAG 295 297 . . 267 271 + BlsI GCNGC 269 268 . . 267 271 + BisI GCNGC 268 269 . . 267 271 + BbvI GCAGC 279 283 . . 267 271 + TseI GCWGC 267 270 . . 272 275 + TspEI AATT 271 275 . . 280 290 + BsiYI* CCNNNNNNNGG 286 283 . . 280 290 + PflMI CCANNNNNTGG 286 283 . . 281 289 + DraIII CACNNNGTG 286 283 . . 285 288 + SetI ASST 288 284 . . 288 293 + CfrI YGGCCR 288 292 . . 289 292 + CviJI RGCY 290 290 . . 289 292 + HaeIII GGCC 290 290 . . 291 296 + DsaI* CCRYGG 291 295 . . 291 296 + SecI* CCNNGG 291 295 . . 290 294 - BceAI ACGGC 275 277 . . 298 301 + TspEI AATT 297 301 . . 325 328 + TspEI AATT 324 328 . . 329 332 + CviRI* TGCA 330 330 . . 335 340 + EcoRV GATATC 337 337 . . 342 345 + SetI ASST 345 341 . . 342 345 + CviJI RGCY 343 343 . . 342 345 + AluI AGCT 343 343 . . 350 355 + MmeI TCCRAC 375 373 . . 357 361 + Tsp4CI* ACNGT 359 358 . . 360 364 + BsmAI GTCTC 365 369 . . 362 367 + SmlI CTYRAG 362 366 . . 366 369 + SetI ASST 369 365 . . 362 367 - Bce83I* CTTGAG 347 345 . . 393 403 + MwoI GCNNNNNNNGC 399 396 . . 402 407 + Cac8I GCNNGC 404 404 . . 405 408 + CviJI RGCY 406 406 . . 405 408 + HaeIII GGCC 406 406 . . 406 410 + TauI GCSGC 409 406 . . 406 410 + BlsI GCNGC 408 407 . . 406 410 + BisI GCNGC 407 408 . . 407 410 + AciI CCGC 407 409 . . 407 412 + BsrBI CCGCTC 409 409 . . 423 427 + MboII GAAGA 435 434 . . 423 428 + BbvII* GAAGAC 430 434 . . 433 438 + HindIII AAGCTT 433 437 . . 434 437 + SetI ASST 437 433 . . 434 437 + CviJI RGCY 435 435 . . 434 437 + AluI AGCT 435 435 . . 439 443 + BsmAI GTCTC 444 448 . . 441 446 - Eco57MI CTGRAG 426 424 . . 441 446 - GsuI CTGGAG 426 424 . . 443 447 - BsrI ACTGG 443 441 . . 448 453 + BetI* WCCGGW 448 452 . . 449 452 + HpaII CCGG 449 451 . . 461 466 + BalI TGGCCA 463 463 . . 461 466 + CfrI YGGCCR 461 465 . . 462 465 + CviJI RGCY 463 463 . . 462 465 + HaeIII GGCC 463 463 . . 479 482 + SetI ASST 482 478 . . 477 481 - Hin4II* CCTTC 471 470 . . 492 495 + SetI ASST 495 491 . . 492 497 + BspMI ACCTGC 501 505 . . 499 503 + TfiI GAWTC 499 502 . . 499 503 + HinfI GANTC 499 502 . . 502 506 + Hpy188I TCNGA 504 503 . . 514 517 - MnlI CCTC 507 506 . . 517 521 + MboII GAAGA 529 528 . . 517 522 - Ksp632I* CTCTTC 512 515 . . 527 532 - EcoP15I CAGCAG 499 501 . . 554 557 + CviJI RGCY 555 555 . . 563 566 + SetI ASST 566 562 . . 566 569 + TaqI TCGA 566 568 . . 568 572 + MboII GAAGA 580 579 . . 578 581 + MseI TTAA 578 580 . . 574 578 - BsrI ACTGG 574 572 . . 586 591 + BseRI GAGGAG 601 599 . . 586 589 - MnlI CCTC 579 578 . . 599 604 + Hpy178III* TCNNGA 600 602 . . 595 599 - BsrI ACTGG 595 593 . . 605 610 + Eco57MI CTGRAG 626 624 . . 605 610 + Eco57I CTGAAG 626 624 . . 607 611 + MboII GAAGA 619 618 . . 616 620 + TspGWI ACGGA 631 629 . . 619 622 - MnlI CCTC 612 611 . . 628 632 + Hpy188I TCNGA 630 629 . . 619 629 - Hin4I GAYNNNNNVTC 642 637 610 605 641 644 + SetI ASST 644 640 . . 646 650 + MboII GAAGA 658 657 . . 646 651 + BbvII* GAAGAC 653 657 . . 650 653 + SetI ASST 653 649 . . 653 656 + MseI TTAA 653 655 . . 661 665 + Hpy188I TCNGA 663 662 . . 664 668 + TfiI GAWTC 664 667 . . 664 668 + HinfI GANTC 664 667 . . 694 698 + MaeIII GTNAC 693 698 . . 699 705 + TspRI CASTGNN 705 696 . . 698 702 - BsrI ACTGG 698 696 . . 706 710 - SfaNI GCATC 696 700 . . 713 718 + BclI TGATCA 713 717 . . 714 717 + DpnI GATC 715 715 . . 714 717 + BstKTI GATC 716 714 . . 714 717 + MboI GATC 713 717 . . 727 731 + MboII GAAGA 739 738 . . 730 734 + MboII GAAGA 742 741 . . 738 742 + FokI GGATG 751 755 . . 738 742 + BseGI GGATG 744 742 . . 751 756 + Hpy166II GTNNAC 753 753 . . 751 756 + HpaI GTTAAC 753 753 . . 751 756 + HindII GTYRAC 753 753 . . 752 755 + MseI TTAA 752 754 . . 775 778 + DpnI GATC 776 776 . . 775 778 + BstKTI GATC 777 775 . . 775 778 + MboI GATC 774 778 . . 779 782 + TaiI ACGT 782 778 . . 779 782 + SetI ASST 782 778 . . 779 782 + MaeII ACGT 779 781 . . 787 790 + MseI TTAA 787 789 . . 789 792 + TspEI AATT 788 792 . . 795 798 + FatI CATG 794 798 . . 795 798 + NlaIII CATG 798 794 . . 795 798 + CviAII CATG 795 797 . . 793 797 - TspDTI ATGAA 783 781 . . 802 811 + MslI CAYNNNNRTG 806 806 . . 802 805 + FatI CATG 801 805 . . 802 805 + NlaIII CATG 805 801 . . 802 805 + CviAII CATG 802 804 . . 811 816 + Cfr10I RCCGGY 811 815 . . 808 812 - SfaNI GCATC 798 802 . . 812 815 + HpaII CCGG 812 814 . . 827 832 - TaqII CACCCA 817 815 . . 850 854 + BsrI ACTGG 855 853 . . 853 856 + CviJI RGCY 854 854 . . 884 888 + TspDTI ATGAA 899 897 . . 894 897 + CviJI RGCY 895 895 . . 895 905 + MwoI GCNNNNNNNGC 901 898 . . 896 901 + StyI CCWWGG 896 900 . . 896 901 + SecI* CCNNGG 896 900 . . 900 904 + FokI GGATG 913 917 . . 900 904 + BseGI GGATG 906 904 . . 903 906 + CviRI* TGCA 904 904 . . 904 909 + Cac8I GCNNGC 906 906 . . 901 905 - SfaNI GCATC 891 895 . . 915 918 + SetI ASST 918 914 . . 921 924 + Csp6I GTAC 921 923 . . 921 924 + RsaI GTAC 922 922 . . 929 932 + FatI CATG 928 932 . . 929 932 + NlaIII CATG 932 928 . . 929 932 + CviAII CATG 929 931 . . 936 939 + FatI CATG 935 939 . . 936 939 + NlaIII CATG 939 935 . . 936 939 + CviAII CATG 936 938 . . 939 944 - BciVI GTATCC 933 932 . . 952 956 + MboII GAAGA 964 963 . . 964 968 - BccI CCATC 958 959 . . 977 980 + TaqI TCGA 977 979 . . 988 998 + FalI AAGNNNNNCTT 979 974 1011 1006 989 994 + StuI AGGCCT 991 991 . . 990 993 + CviJI RGCY 991 991 . . 990 993 + HaeIII GGCC 991 991 . . 988 998 - FalI AAGNNNNNCTT 1011 1006 979 974 1029 1033 + FokI GGATG 1042 1046 . . 1029 1033 + BseGI GGATG 1035 1033 . . 1030 1034 - SfaNI GCATC 1020 1024 . . 1036 1040 + BssKI CCNGG 1035 1040 . . 1036 1040 + BseBI CCWGG 1037 1038 . . 1036 1040 + ScrFI CCNGG 1037 1038 . . 1036 1040 + EcoRII CCWGG 1035 1040 . . 1050 1053 + Csp6I GTAC 1050 1052 . . 1050 1053 + RsaI GTAC 1051 1051 . . 1052 1055 + TaiI ACGT 1055 1051 . . 1052 1055 + SetI ASST 1055 1051 . . 1052 1055 + MaeII ACGT 1052 1054 . . 1057 1061 + Hpy188I TCNGA 1059 1058 . . 1065 1068 + DpnI GATC 1066 1066 . . 1065 1068 + BstKTI GATC 1067 1065 . . 1065 1068 + MboI GATC 1064 1068 . . 1075 1085 + MwoI GCNNNNNNNGC 1081 1078 . . 1079 1084 + SmlI CTYRAG 1079 1083 . . 1083 1086 + SetI ASST 1086 1082 . . 1083 1086 + CviJI RGCY 1084 1084 . . 1083 1086 + AluI AGCT 1084 1084 . . 1079 1084 - Bce83I* CTTGAG 1064 1062 . . 1109 1112 + AciI CCGC 1109 1111 . . 1108 1113 - EciI GGCGGA 1098 1096 . . 1120 1124 + HphI GGTGA 1132 1131 . . 1125 1128 + Csp6I GTAC 1125 1127 . . 1125 1128 + RsaI GTAC 1126 1126 . . 1132 1137 + Cfr10I RCCGGY 1132 1136 . . 1132 1137 + BetI* WCCGGW 1132 1136 . . 1132 1137 + AgeI ACCGGT 1132 1136 . . 1133 1136 + HpaII CCGG 1133 1135 . . 1146 1149 + SetI ASST 1149 1145 . . 1145 1148 - MnlI CCTC 1138 1137 . . 1143 1153 - Hin4I GAYNNNNNVTC 1166 1161 1134 1129 1158 1163 + Hpy178III* TCNNGA 1159 1161 . . 1160 1165 + Hpy166II GTNNAC 1162 1162 . . 1157 1162 - BsiI* CACGAG 1157 1161 . . 1168 1178 + MwoI GCNNNNNNNGC 1174 1171 . . 1168 1178 + BglI GCCNNNNNGGC 1174 1171 . . 1175 1180 + BalI TGGCCA 1177 1177 . . 1175 1180 + CfrI YGGCCR 1175 1179 . . 1176 1179 + CviJI RGCY 1177 1177 . . 1176 1179 + HaeIII GGCC 1177 1177 . . 1185 1190 + StyI CCWWGG 1185 1189 . . 1185 1190 + SecI* CCNNGG 1185 1189 . . 1188 1191 + SetI ASST 1191 1187 . . 1193 1196 + MseI TTAA 1193 1195 . . 1200 1204 - BccI CCATC 1194 1195 . . 1209 1213 + Hpy99I CGWCG 1213 1208 . . 1224 1228 + FokI GGATG 1237 1241 . . 1224 1228 + BseGI GGATG 1230 1228 . . 1221 1225 - BccI CCATC 1215 1216 . . 1231 1245 + XcmI CCANNNNNNNNNTGG 1238 1237 . . 1234 1238 + Tsp4CI* ACNGT 1236 1235 . . 1235 1245 + BsiYI* CCNNNNNNNGG 1241 1238 . . 1237 1242 + Hpy166II GTNNAC 1239 1239 . . 1237 1242 + HpaI GTTAAC 1239 1239 . . 1237 1242 + HindII GTYRAC 1239 1239 . . 1238 1241 + MseI TTAA 1238 1240 . . 1241 1245 + BsrI ACTGG 1246 1244 . . 1247 1251 + DdeI CTNAG 1247 1250 . . 1253 1258 + AclI AACGTT 1254 1256 . . 1254 1257 + TaiI ACGT 1257 1253 . . 1254 1257 + SetI ASST 1257 1253 . . 1254 1257 + MaeII ACGT 1254 1256 . . 1256 1260 + MaeIII GTNAC 1255 1260 . . 1271 1282 + BdaI TGANNNNNNTCA 1260 1258 1294 1292 1271 1282 - BdaI TGANNNNNNTCA 1294 1292 1260 1258 1285 1288 + TspEI AATT 1284 1288 . . 1289 1294 + Hpy178III* TCNNGA 1290 1292 . . 1290 1295 + SmlI CTYRAG 1290 1294 . . 1290 1295 + Bce83I* CTTGAG 1311 1309 . . 1298 1303 + MfeI CAATTG 1298 1302 . . 1299 1302 + TspEI AATT 1298 1302 . . 1304 1308 + MaeIII GTNAC 1303 1308 . . 1302 1307 - TsoI TARCCA 1292 1290 . . 1313 1316 + MseI TTAA 1313 1315 . . 1322 1325 + TaiI ACGT 1325 1321 . . 1322 1325 + SetI ASST 1325 1321 . . 1322 1325 + MaeII ACGT 1322 1324 . . 1336 1346 + BsiYI* CCNNNNNNNGG 1342 1339 . . 1339 1344 + BciVI GTATCC 1350 1349 . . 1336 1340 - BsrI ACTGG 1336 1334 . . 1343 1346 + HpaII CCGG 1343 1345 . . 1345 1348 + CviJI RGCY 1346 1346 . . 1347 1352 + SfeI* CTRYAG 1347 1351 . . 1349 1353 + Tsp4CI* ACNGT 1351 1350 . . 1350 1356 + TspRI CASTGNN 1356 1347 . . 1356 1359 + CviRI* TGCA 1357 1357 . . 1359 1364 + ApoI RAATTY 1359 1363 . . 1360 1363 + TspEI AATT 1359 1363 . . 1369 1372 + DpnI GATC 1370 1370 . . 1369 1372 + BstKTI GATC 1371 1369 . . 1369 1372 + MboI GATC 1368 1372 . . 1372 1377 + BsaAI YACGTR 1374 1374 . . 1373 1376 + TaiI ACGT 1376 1372 . . 1373 1376 + SetI ASST 1376 1372 . . 1373 1376 + MaeII ACGT 1373 1375 . . 1377 1382 + XhoII RGATCY 1377 1381 . . 1378 1381 + DpnI GATC 1379 1379 . . 1378 1381 + BstKTI GATC 1380 1378 . . 1378 1381 + MboI GATC 1377 1381 . . 1378 1382 - BinI* GGATC 1372 1373 . . 1387 1392 + BsmI GAATGC 1393 1391 . . 1393 1398 + DsaI* CCRYGG 1393 1397 . . 1393 1398 + StyI CCWWGG 1393 1397 . . 1393 1398 + NcoI CCATGG 1393 1397 . . 1393 1398 + SecI* CCNNGG 1393 1397 . . 1394 1397 + FatI CATG 1393 1397 . . 1394 1397 + NlaIII CATG 1397 1393 . . 1394 1397 + CviAII CATG 1394 1396 . . 1398 1403 + Hpy166II GTNNAC 1400 1400 . . 1398 1401 + Csp6I GTAC 1398 1400 . . 1398 1401 + RsaI GTAC 1399 1399 . . 1402 1407 + Cfr10I RCCGGY 1402 1406 . . 1403 1406 + HpaII CCGG 1403 1405 . . 1405 1408 + CviJI RGCY 1406 1406 . . 1405 1408 + HaeIII GGCC 1406 1406 . . 1405 1409 + BmgT120I GGNCC 1406 1407 . . 1405 1409 + AsuI* GGNCC 1405 1408 . . 1425 1430 + Hpy178III* TCNNGA 1426 1428 . . 1428 1433 - BciVI GTATCC 1422 1421 . . 1435 1439 + TspDTI ATGAA 1450 1448 . . 1441 1446 + BtrI CACGTC 1443 1443 . . 1442 1445 + TaiI ACGT 1445 1441 . . 1442 1445 + SetI ASST 1445 1441 . . 1442 1445 + MaeII ACGT 1442 1444 . . 1443 1447 + Hpy99I CGWCG 1447 1442 . . 1444 1449 + SalI GTCGAC 1444 1448 . . 1444 1449 + Hpy166II GTNNAC 1446 1446 . . 1444 1449 + HindII GTYRAC 1446 1446 . . 1444 1449 + AccI GTMKAC 1445 1447 . . 1445 1448 + TaqI TCGA 1445 1447 . . 1446 1451 + McrI* CGRYCG 1449 1447 . . 1448 1452 + Tsp4CI* ACNGT 1450 1449 . . 1451 1455 + Tsp45I GTSAC 1450 1455 . . 1451 1455 + MaeIII GTNAC 1450 1455 . . 1453 1462 + MslI CAYNNNNRTG 1457 1457 . . 1470 1473 + FatI CATG 1469 1473 . . 1470 1473 + NlaIII CATG 1473 1469 . . 1470 1473 + CviAII CATG 1470 1472 . . 1468 1472 - TspDTI ATGAA 1458 1456 . . 1483 1487 + TauI GCSGC 1486 1483 . . 1483 1487 + BlsI GCNGC 1485 1484 . . 1483 1487 + BisI GCNGC 1484 1485 . . 1484 1487 + AciI CCGC 1484 1486 . . 1487 1491 + DdeI CTNAG 1487 1490 . . 1491 1501 + Hin4I GAYNNNNNVTC 1482 1477 1514 1509 1492 1496 + TspDTI ATGAA 1507 1505 . . 1497 1502 + XhoII RGATCY 1497 1501 . . 1497 1501 + BinI* GGATC 1505 1506 . . 1494 1498 - Hin4II* CCTTC 1488 1487 . . 1498 1501 + DpnI GATC 1499 1499 . . 1498 1501 + BstKTI GATC 1500 1498 . . 1498 1501 + MboI GATC 1497 1501 . . 1491 1501 - Hin4I GAYNNNNNVTC 1514 1509 1482 1477 1501 1504 + MaeI CTAG 1501 1503 . . 1503 1506 + SetI ASST 1506 1502 . . 1504 1509 + KpnI GGTACC 1508 1504 . . 1504 1509 + NlaIV GGNNCC 1506 1506 . . 1504 1509 + HgiCI* GGYRCC 1504 1508 . . 1504 1509 + Acc65I GGTACC 1504 1508 . . 1505 1508 + Csp6I GTAC 1505 1507 . . 1505 1508 + RsaI GTAC 1506 1506 . . 1508 1512 + BccI CCATC 1516 1517 . . 1504 1514 - BaeI ACNNNNGTAYC 1529 1524 1496 1491 1518 1521 + SetI ASST 1521 1517 . . 1516 1520 - Hin4II* CCTTC 1510 1509 . . 1524 1527 + TspEI AATT 1523 1527 . . 1527 1538 + BdaI TGANNNNNNTCA 1516 1514 1550 1548 1528 1532 + TfiI GAWTC 1528 1531 . . 1528 1532 + HinfI GANTC 1528 1531 . . 1531 1536 + BetI* WCCGGW 1531 1535 . . 1532 1535 + HpaII CCGG 1532 1534 . . 1527 1538 - BdaI TGANNNNNNTCA 1550 1548 1516 1514 1566 1570 - SfaNI GCATC 1556 1560 . . 1580 1585 + NspBII* CMGCKG 1582 1582 . . 1580 1583 + AciI CCGC 1580 1582 . . 1589 1594 + ApoI RAATTY 1589 1593 . . 1590 1593 + TspEI AATT 1589 1593 . . 1597 1602 + SspI AATATT 1599 1599 . . 1619 1623 + TspDTI ATGAA 1634 1632 . . 1632 1635 + CviJI RGCY 1633 1633 . . 1631 1636 - TsoI TARCCA 1621 1619 . . 1642 1646 + HphI GGTGA 1654 1653 . . 1652 1655 + MseI TTAA 1652 1654 . . 1657 1661 + DdeI CTNAG 1657 1660 . . 1661 1665 + TfiI GAWTC 1661 1664 . . 1661 1665 + HinfI GANTC 1661 1664 . . 1668 1671 + SetI ASST 1671 1667 . . 1675 1679 + MboII GAAGA 1687 1686 . . 1678 1682 + MboII GAAGA 1690 1689 . . 1681 1685 + MboII GAAGA 1693 1692 . . 1681 1686 + BbvII* GAAGAC 1688 1692 . . 1692 1698 + SexAI ACCWGGT 1692 1697 . . 1693 1697 + BssKI CCNGG 1692 1697 . . 1693 1697 + BseBI CCWGG 1694 1695 . . 1693 1697 + ScrFI CCNGG 1694 1695 . . 1693 1697 + EcoRII CCWGG 1692 1697 . . 1690 1694 - HphI GGTGA 1682 1681 . . 1695 1698 + SetI ASST 1698 1694 . . 1701 1706 + TatI WGTACW 1701 1705 . . 1702 1705 + Csp6I GTAC 1702 1704 . . 1702 1705 + RsaI GTAC 1703 1703 . . 1709 1714 - BtgZI GCGATG 1694 1698 . . 1727 1732 + Hpy178III* TCNNGA 1728 1730 . . 1735 1739 + MaeIII GTNAC 1734 1739 . . 1749 1752 + SetI ASST 1752 1748 . . 1749 1752 + CviJI RGCY 1750 1750 . . 1749 1752 + AluI AGCT 1750 1750 . . 1750 1760 + MwoI GCNNNNNNNGC 1756 1753 . . 1755 1758 + TspEI AATT 1754 1758 . . 1769 1772 + TspEI AATT 1768 1772 . . 1771 1774 + MseI TTAA 1771 1773 . . 1786 1790 + BlsI GCNGC 1788 1787 . . 1786 1790 + BisI GCNGC 1787 1788 . . 1786 1790 + BbvI GCAGC 1798 1802 . . 1786 1790 + TseI GCWGC 1786 1789 . . 1788 1791 + CviJI RGCY 1789 1789 . . 1789 1794 + Cfr10I RCCGGY 1789 1793 . . 1790 1793 + HpaII CCGG 1790 1792 . . 1799 1802 + FatI CATG 1798 1802 . . 1799 1802 + NlaIII CATG 1802 1798 . . 1799 1802 + CviAII CATG 1799 1801 . . 1797 1801 - TspDTI ATGAA 1787 1785 . . 1807 1812 + EcoT22I ATGCAT 1811 1807 . . 1808 1811 + CviRI* TGCA 1809 1809 . . 1809 1814 + NspI RCATGY 1813 1809 . . 1810 1813 + FatI CATG 1809 1813 . . 1810 1813 + NlaIII CATG 1813 1809 . . 1810 1813 + CviAII CATG 1810 1812 . . 1814 1818 - TspDTI ATGAA 1804 1802 . . 1823 1828 + MfeI CAATTG 1823 1827 . . 1824 1827 + TspEI AATT 1823 1827 . . 1828 1832 + MboII GAAGA 1840 1839 . . 1832 1835 + SetI ASST 1835 1831 . . 1828 1833 - Ksp632I* CTCTTC 1823 1826 . . 1831 1834 - MnlI CCTC 1824 1823 . . 1834 1837 + Csp6I GTAC 1834 1836 . . 1834 1837 + RsaI GTAC 1835 1835 . . 1844 1847 + MseI TTAA 1844 1846 . . 1853 1856 + CviRI* TGCA 1854 1854 . . 1859 1862 + TspEI AATT 1858 1862 . . 1870 1875 + KpnI GGTACC 1874 1870 . . 1870 1875 + NlaIV GGNNCC 1872 1872 . . 1870 1875 + HgiCI* GGYRCC 1870 1874 . . 1870 1875 + Acc65I GGTACC 1870 1874 . . 1871 1874 + Csp6I GTAC 1871 1873 . . 1871 1874 + RsaI GTAC 1872 1872 . . 1877 1881 + Tsp4CI* ACNGT 1879 1878 . . 1895 1901 + AarI CACCTGC 1905 1909 . . 1896 1899 + SetI ASST 1899 1895 . . 1896 1901 + BspMI ACCTGC 1905 1909 . . 1907 1911 + DdeI CTNAG 1907 1910 . . 1911 1915 - Hin4II* CCTTC 1905 1904 . . 1918 1922 + TspDTI ATGAA 1933 1931 . . 1922 1925 + SetI ASST 1925 1921 . . 1920 1924 - Hin4II* CCTTC 1914 1913 . . 1926 1931 - BtgZI GCGATG 1911 1915 . . 1942 1952 + BsaXI ACNNNNNCTCC 1932 1929 1962 1959 1943 1948 + Eco57MI CTGRAG 1964 1962 . . 1943 1948 + Eco57I CTGAAG 1964 1962 . . 1947 1950 + SetI ASST 1950 1946 . . 1947 1950 + CviJI RGCY 1948 1948 . . 1947 1950 + AluI AGCT 1948 1948 . . 1949 1954 - Eco57MI CTGRAG 1934 1932 . . 1949 1954 - GsuI CTGGAG 1934 1932 . . 1951 1955 - BsrI ACTGG 1951 1949 . . 1981 1984 + MnlI CCTC 1991 1990 . . 1981 1991 + EcoNI CCTNNNNNAGG 1985 1986 . . 1981 1991 + BsiYI* CCNNNNNNNGG 1987 1984 . . 1985 1988 + TspEI AATT 1984 1988 . . 1989 1992 + SetI ASST 1992 1988 . . 1994 1998 + TfiI GAWTC 1994 1997 . . 1994 1998 + HinfI GANTC 1994 1997 . . 2008 2011 + DpnI GATC 2009 2009 . . 2008 2011 + BstKTI GATC 2010 2008 . . 2008 2011 + MboI GATC 2007 2011 . . 2013 2016 + SetI ASST 2016 2012 . . 2014 2019 + KpnI GGTACC 2018 2014 . . 2014 2019 + NlaIV GGNNCC 2016 2016 . . 2014 2019 + HgiCI* GGYRCC 2014 2018 . . 2014 2019 + Acc65I GGTACC 2014 2018 . . 2015 2018 + Csp6I GTAC 2015 2017 . . 2015 2018 + RsaI GTAC 2016 2016 . . 2027 2032 + MfeI CAATTG 2027 2031 . . 2028 2031 + TspEI AATT 2027 2031 . . 2037 2040 + TspEI AATT 2036 2040 . . 2042 2045 + MseI TTAA 2042 2044 . . 2046 2049 + TspEI AATT 2045 2049 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 115 153 1790 # # Acc65I: # [GGTACC] # 44 144 161 366 1343 # # AccI: # [GTMKAC] # 613 1445 # # AciI: # [CCGC] # 96 150 257 375 478 702 # # AclI: # [AACGTT] # 804 1254 # # AcyI: # [GRCGYC] # 197 1861 # # AgeI: # [ACCGGT] # 926 1132 # # AluI: # [AGCT] # 92 100 110 198 243 649 # 666 # # ApoI: # [RAATTY] # 230 469 1359 # # AsuI*: # [GGNCC] # 653 1405 # # BaeI: # [ACNNNNGTAYC] # 33 33 75 529 1388 # # BalI: # [TGGCCA] # 463 714 881 # # BbvI: # [GCAGC] # 260 279 1519 # # BbvII*: # [GAAGAC] # 223 370 430 1035 # # BccI: # [CCATC] # 21 236 301 542 958 # # Bce83I*: # [CTTGAG] # 27 95 111 114 247 717 # 747 # # BceAI: # [ACGGC] # 275 1783 # # BciVI: # [GTATCC] # 72 417 636 933 # # BclI: # [TGATCA] # 713 1345 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 222 508 1260 # # BetI*: # [WCCGGW] # 399 448 527 684 # # BglI: # [GCCNNNNNGGC] # 884 1174 # # BinI*: # [GGATC] # 133 553 1372 # # BisI: # [GCNGC] # 139 268 271 303 1077 # # BlsI: # [GCNGC] # 139 269 270 303 1077 # # BmgT120I: # [GGNCC] # 652 1406 # # BsaAI: # [YACGTR] # 684 1374 # # BsaXI: # [ACNNNNNCTCC] # 30 96 1932 # # BseBI: # [CCWGG] # 364 657 1037 # # BseGI: # [GGATG] # 129 162 195 744 828 # # BseRI: # [GAGGAG] # 601 1457 # # BsiI*: # [CACGAG] # 901 1157 # # BsiYI*: # [CCNNNNNNNGG] # 27 30 30 71 72 101 # 127 645 955 # # BsmAI: # [GTCTC] # 79 365 1614 # # BsmI: # [GAATGC] # 665 1393 # # BspMI: # [ACCTGC] # 14 115 153 372 1404 # # BsrBI: # [CCGCTC] # 409 1649 # # BsrI: # [ACTGG] # 21 90 103 107 131 157 # 391 443 615 # # BssKI: # [CCNGG] # 366 657 1035 # # BstEII: # [GGTNACC] # 58 144 1856 # # BstKTI: # [GATC] # 9 48 61 120 290 304 # 510 716 # # BstXI: # [CCANNNNNNTGG] # 172 1886 # # BtgZI: # [GCGATG] # 147 217 1694 # # BtrI: # [CACGTC] # 615 1443 # # Cac8I: # [GCNNGC] # 404 502 1152 # # Cfr10I: # [RCCGGY] # 199 269 270 321 387 612 # # CfrI: # [YGGCCR] # 173 288 714 883 # # Csp6I: # [GTAC] # 24 30 37 43 57 75 # 107 129 132 132 144 197 # 273 678 # # CviAII: # [CATG] # 7 7 11 76 127 248 # 329 458 795 # # CviJI: # [RGCY] # 15 28 29 39 41 50 # 50 53 57 57 60 61 # 63 92 93 93 96 110 # 117 159 169 227 299 # # CviRI*: # [TGCA] # 45 124 204 206 452 453 # 574 # # DdeI: # [CTNAG] # 151 170 240 250 1247 # # DpnI: # [GATC] # 9 49 61 120 290 304 # 510 715 # # DraIII: # [CACNNNGTG] # 286 1772 # # DsaI*: # [CCRYGG] # 291 665 1102 # # EciI: # [GGCGGA] # 960 1098 # # Eco57I: # [CTGAAG] # 94 626 1338 # # Eco57MI: # [CTGRAG] # 30 94 200 426 1308 # # EcoNI: # [CCTNNNNNAGG] # 73 227 1758 # # EcoP15I: # [CAGCAG] # 204 295 1559 # # EcoRII: # [CCWGG] # 366 657 1035 # # EcoRV: # [GATATC] # 337 1721 # # EcoT22I: # [ATGCAT] # 247 1811 # # FalI: # [AAGNNNNNCTT] # 0 0 32 979 1047 # # FatI: # [CATG] # 7 7 11 76 127 249 # 329 458 794 # # FokI: # [GGATG] # 129 162 195 751 821 # # GsuI: # [CTGGAG] # 124 426 1508 # # HaeIII: # [GGCC] # 57 116 186 229 290 528 # 652 # # HgaI: # [GACGC] # 205 1853 # # HgiCI*: # [GGYRCC] # 44 144 161 366 1343 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 316 # 492 544 610 # # Hin4II*: # [CCTTC] # 9 22 144 395 471 1017 # # HindII: # [GTYRAC] # 207 486 612 753 # # HindIII: # [AAGCTT] # 98 335 1625 # # HinfI: # [GANTC] # 7 64 133 165 333 492 # 864 # # HpaI: # [GTTAAC] # 486 753 819 # # HpaII: # [CCGG] # 60 129 200 210 249 258 # 268 321 363 # # HphI: # [GGTGA] # 28 376 522 1132 # # Hpy166II: # [GTNNAC] # 46 77 161 409 612 753 # # Hpy178III*: # [TCNNGA] # 131 136 193 302 330 407 # 559 # # Hpy188I: # [TCNGA] # 6 33 126 396 498 999 # # Hpy99I: # [CGWCG] # 199 234 611 1014 # # KpnI: # [GGTACC] # 40 144 165 366 1343 # # Ksp632I*: # [CTCTTC] # 235 512 1311 # # MaeI: # [CTAG] # 557 1501 # # MaeII: # [ACGT] # 51 68 69 202 273 616 # 779 # # MaeIII: # [GTNAC] # 15 48 58 129 147 284 # 324 491 562 # # MboI: # [GATC] # 9 51 61 120 290 304 # 510 713 # # MboII: # [GAAGA] # 3 3 3 39 39 51 # 81 94 147 218 222 435 # 723 # # McrI*: # [CGRYCG] # 609 1449 # # MfeI: # [CAATTG] # 31 204 525 1298 # # MmeI: # [TCCRAC] # 375 1683 # # MnlI: # [CCTC] # 10 27 33 67 72 167 # 223 247 526 686 # # MseI: # [TTAA] # 16 35 45 73 75 75 # 99 119 198 339 406 578 # # MslI: # [CAYNNNNRTG] # 601 651 806 # # MwoI: # [GCNNNNNNNGC] # 15 93 106 180 278 302 # 502 582 # # NcoI: # [CCATGG] # 665 1393 # # NlaIII: # [CATG] # 7 7 11 76 127 245 # 329 458 798 # # NlaIV: # [GGNNCC] # 42 144 163 366 1343 # # NspBII*: # [CMGCKG] # 476 1582 # # NspI: # [RCATGY] # 245 1813 # # PflMI: # [CCANNNNNTGG] # 286 1772 # # PstI: # [CTGCAG] # 126 1932 # # RsaI: # [GTAC] # 24 30 37 42 57 75 # 107 129 132 133 144 197 # 273 678 # # SalI: # [GTCGAC] # 614 1444 # # ScrFI: # [CCNGG] # 364 657 1037 # # SecI*: # [CCNNGG] # 15 60 72 144 208 289 # 605 665 # # SetI: # [ASST] # 6 9 9 13 15 18 # 21 21 24 24 24 25 # 26 27 31 42 42 42 # 45 51 54 57 61 63 # 64 66 68 68 69 71 # 75 78 83 114 129 136 # 137 150 # # SexAI: # [ACCWGGT] # 366 1692 # # SfaNI: # [GCATC] # 93 102 129 502 536 696 # # SfeI*: # [CTRYAG] # 122 711 1225 # # SmlI: # [CTYRAG] # 27 110 111 114 211 717 # 768 # # Sse8387I: # [CCTGCAGG] # 126 1932 # # SspI: # [AATATT] # 459 1599 # # StuI: # [AGGCCT] # 991 1067 # # StyI: # [CCWWGG] # 15 60 72 208 289 665 # 749 # # TaiI: # [ACGT] # 51 68 69 202 273 613 # 782 # # TaqI: # [TCGA] # 411 468 566 613 # # TaqII: # [CACCCA] # 817 1241 # # TatI: # [WGTACW] # 57 185 357 1459 # # TauI: # [GCSGC] # 409 572 1077 # # TfiI: # [GAWTC] # 7 64 133 165 333 492 # 864 # # TseI: # [GCWGC] # 267 272 1519 # # TsoI: # [TARCCA] # 76 88 329 437 1128 # # Tsp45I: # [GTSAC] # 608 1450 # # Tsp4CI*: # [ACNGT] # 27 30 58 99 115 117 # 127 179 429 877 # # TspDTI: # [ATGAA] # 8 17 49 116 125 127 # 129 153 551 783 # # TspEI: # [AATT] # 9 9 13 14 14 26 # 27 35 43 55 61 66 # 126 130 141 164 165 464 # 496 # # TspGWI: # [ACGGA] # 631 1427 # # TspRI: # [CASTGNN] # 651 702 705 # # XcmI: # [CCANNNNNNNNNTGG] # 30 124 820 1084 # # XhoII: # [RGATCY] # 120 561 1377 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2058 # Reported_sequences: 1 # Reported_hitcount: 529 #---------------------------------------