Restriction Fragments for SEC7/YDR170C



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The currently selected gene/sequence is :

SEC7/YDR170C on chromosome IV from coordinates 802222 to 796193.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14337.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + Hpy188I TCNGA 6 5 . . 12 17 + EcoRI GAATTC 12 16 . . 12 17 + ApoI RAATTY 12 16 . . 13 16 + TspEI AATT 12 16 . . 39 43 + FokI GGATG 52 56 . . 39 43 + BseGI GGATG 45 43 . . 40 44 - BccI CCATC 34 35 . . 52 56 - MboII GAAGA 44 43 . . 64 68 - BsmAI GTCTC 58 62 . . 73 77 - MboII GAAGA 65 64 . . 74 79 - Eco57MI CTGRAG 59 57 . . 74 79 - Eco57I CTGAAG 59 57 . . 84 88 - TspGWI ACGGA 74 72 . . 102 107 + BsmI GAATGC 108 106 . . 110 113 + MseI TTAA 110 112 . . 115 119 + MboII GAAGA 127 126 . . 120 123 + SetI ASST 123 119 . . 120 123 + CviJI RGCY 121 121 . . 120 123 + AluI AGCT 121 121 . . 127 131 - BsmAI GTCTC 121 125 . . 136 140 + HphI GGTGA 148 147 . . 139 143 + MboII GAAGA 151 150 . . 142 145 + DpnI GATC 143 143 . . 142 145 + BstKTI GATC 144 142 . . 142 145 + MboI GATC 141 145 . . 151 154 + CviRI* TGCA 152 152 . . 157 161 + BmgT120I GGNCC 158 159 . . 157 161 + AsuI* GGNCC 157 160 . . 158 161 + CviJI RGCY 159 159 . . 158 161 + HaeIII GGCC 159 159 . . 169 174 + Cac8I GCNNGC 171 171 . . 176 179 + Csp6I GTAC 176 178 . . 176 179 + RsaI GTAC 177 177 . . 178 182 + BceAI ACGGC 194 196 . . 200 204 + MaeIII GTNAC 199 204 . . 204 208 + Hpy99I CGWCG 208 203 . . 205 209 + HgaI GACGC 214 219 . . 215 219 + TspGWI ACGGA 230 228 . . 220 224 + TspDTI ATGAA 235 233 . . 232 236 + MboII GAAGA 244 243 . . 238 242 + TspGWI ACGGA 253 251 . . 241 245 + MboII GAAGA 253 252 . . 262 271 + MslI CAYNNNNRTG 266 266 . . 262 265 + FatI CATG 261 265 . . 262 265 + NlaIII CATG 265 261 . . 262 265 + CviAII CATG 262 264 . . 269 273 + TspDTI ATGAA 284 282 . . 273 276 + SetI ASST 276 272 . . 274 278 + HphI GGTGA 286 285 . . 271 275 - Hin4II* CCTTC 265 264 . . 277 281 + MboII GAAGA 289 288 . . 280 284 - BccI CCATC 274 275 . . 287 291 + TspDTI ATGAA 302 300 . . 289 293 + MboII GAAGA 301 300 . . 293 297 + TspDTI ATGAA 308 306 . . 295 299 + MboII GAAGA 307 306 . . 299 303 + TspDTI ATGAA 314 312 . . 301 305 + MboII GAAGA 313 312 . . 305 309 + TspDTI ATGAA 320 318 . . 307 311 + MboII GAAGA 319 318 . . 311 315 + TspDTI ATGAA 326 324 . . 313 317 + MboII GAAGA 325 324 . . 317 321 + TspDTI ATGAA 332 330 . . 319 323 + MboII GAAGA 331 330 . . 319 324 + BbvII* GAAGAC 326 330 . . 329 333 + BslFI GGGAC 343 347 . . 336 340 + FokI GGATG 349 353 . . 336 340 + BseGI GGATG 342 340 . . 334 337 - MnlI CCTC 327 326 . . 343 346 - MnlI CCTC 336 335 . . 347 350 + TaiI ACGT 350 346 . . 347 350 + SetI ASST 350 346 . . 347 350 + MaeII ACGT 347 349 . . 346 351 - BtrI CACGTC 348 348 . . 364 368 + Hpy188I TCNGA 366 365 . . 371 375 - BsrI ACTGG 371 369 . . 376 380 + Hpy188I TCNGA 378 377 . . 377 387 + BsiYI* CCNNNNNNNGG 383 380 . . 379 383 + MboII GAAGA 391 390 . . 379 384 + BbvII* GAAGAC 386 390 . . 388 392 + MboII GAAGA 400 399 . . 391 395 + TfiI GAWTC 391 394 . . 391 395 + HinfI GANTC 391 394 . . 391 401 + Hin4I GAYNNNNNVTC 382 377 414 409 394 398 + Hpy188I TCNGA 396 395 . . 397 401 + TfiI GAWTC 397 400 . . 397 401 + HinfI GANTC 397 400 . . 391 401 - Hin4I GAYNNNNNVTC 414 409 382 377 403 407 + BsmAI GTCTC 408 412 . . 405 409 + DdeI CTNAG 405 408 . . 405 409 + BspCNI CTCAG 418 416 . . 405 409 + BseMII CTCAG 419 417 . . 415 420 + TatI WGTACW 415 419 . . 415 420 + ScaI AGTACT 417 417 . . 416 419 + Csp6I GTAC 416 418 . . 416 419 + RsaI GTAC 417 417 . . 419 423 + DdeI CTNAG 419 422 . . 421 431 + BplI GAGNNNNNCTC 412 407 444 439 419 423 - BspCNI CTCAG 411 409 . . 419 423 - BseMII CTCAG 410 408 . . 427 430 + SetI ASST 430 426 . . 427 430 + CviJI RGCY 428 428 . . 427 430 + AluI AGCT 428 428 . . 430 435 + Hpy178III* TCNNGA 431 433 . . 431 436 + Eco57MI CTGRAG 452 450 . . 431 436 + GsuI CTGGAG 452 450 . . 421 431 - BplI GAGNNNNNCTC 444 439 412 407 436 440 + MboII GAAGA 448 447 . . 439 449 + Hin4I GAYNNNNNVTC 430 425 462 457 440 444 + TspDTI ATGAA 455 453 . . 442 446 + MboII GAAGA 454 453 . . 445 449 + TfiI GAWTC 445 448 . . 445 449 + HinfI GANTC 445 448 . . 448 452 + Hpy188I TCNGA 450 449 . . 439 449 - Hin4I GAYNNNNNVTC 462 457 430 425 452 456 + TspDTI ATGAA 467 465 . . 458 463 + BtgZI GCGATG 473 477 . . 460 464 - BccI CCATC 454 455 . . 478 481 + SetI ASST 481 477 . . 478 481 + CviJI RGCY 479 479 . . 478 481 + AluI AGCT 479 479 . . 482 485 + MnlI CCTC 492 491 . . 480 485 - BseRI GAGGAG 471 469 . . 487 491 + HphI GGTGA 499 498 . . 491 495 + TspDTI ATGAA 506 504 . . 497 500 - AciI CCGC 497 499 . . 497 501 - FauI CCCGC 490 492 . . 502 506 + Hpy188I TCNGA 504 503 . . 505 509 + MboII GAAGA 517 516 . . 508 512 + MboII GAAGA 520 519 . . 511 515 + MboII GAAGA 523 522 . . 514 518 + MboII GAAGA 526 525 . . 517 521 + MboII GAAGA 529 528 . . 520 524 + MboII GAAGA 532 531 . . 523 527 + MboII GAAGA 535 534 . . 526 530 + MboII GAAGA 538 537 . . 529 533 + MboII GAAGA 541 540 . . 532 536 + MboII GAAGA 544 543 . . 535 539 + MboII GAAGA 547 546 . . 538 542 + MboII GAAGA 550 549 . . 538 543 - Ksp632I* CTCTTC 533 536 . . 541 544 - MnlI CCTC 534 533 . . 550 555 + Cac8I GCNNGC 552 552 . . 561 564 + SetI ASST 564 560 . . 561 564 + CviJI RGCY 562 562 . . 561 564 + AluI AGCT 562 562 . . 573 578 + Hpy178III* TCNNGA 574 576 . . 594 605 + BcgI CGANNNNNNTGC 583 581 617 615 595 599 + TfiI GAWTC 595 598 . . 595 599 + HinfI GANTC 595 598 . . 607 612 + StyI CCWWGG 607 611 . . 607 612 + SecI* CCNNGG 607 611 . . 610 613 + SetI ASST 613 609 . . 612 617 + Hpy166II GTNNAC 614 614 . . 612 617 + AccI GTMKAC 613 615 . . 617 627 + BsiYI* CCNNNNNNNGG 623 620 . . 624 627 - MnlI CCTC 617 616 . . 640 644 - MboII GAAGA 632 631 . . 651 656 + AsuII TTCGAA 652 654 . . 652 655 + TaqI TCGA 652 654 . . 654 665 + PpiI GAACNNNNNCTC 646 641 678 673 672 675 + TspEI AATT 671 675 . . 681 694 + AjuI GAANNNNNNNTTGG 673 668 705 700 688 691 + TaiI ACGT 691 687 . . 688 691 + SetI ASST 691 687 . . 688 691 + MaeII ACGT 688 690 . . 690 695 - MmeI TCCRAC 671 669 . . 694 698 - BsmAI GTCTC 688 692 . . 699 702 + TspEI AATT 698 702 . . 702 707 + AhaIII* TTTAAA 704 704 . . 703 706 + MseI TTAA 703 705 . . 719 722 + MseI TTAA 719 721 . . 726 731 + EcoRI GAATTC 726 730 . . 726 731 + ApoI RAATTY 726 730 . . 727 730 + TspEI AATT 726 730 . . 729 734 + AsuII TTCGAA 730 732 . . 730 733 + TaqI TCGA 730 732 . . 732 737 + BsmI GAATGC 738 736 . . 736 746 + MwoI GCNNNNNNNGC 742 739 . . 737 741 + DdeI CTNAG 737 740 . . 737 741 + BspCNI CTCAG 750 748 . . 737 741 + BseMII CTCAG 751 749 . . 738 742 + Hpy188I TCNGA 740 739 . . 744 747 + CviJI RGCY 745 745 . . 741 745 - Hin4II* CCTTC 735 734 . . 749 752 + TaqI TCGA 749 751 . . 766 771 + ApoI RAATTY 766 770 . . 767 770 + TspEI AATT 766 770 . . 770 773 + MseI TTAA 770 772 . . 775 780 + ApoI RAATTY 775 779 . . 776 779 + TspEI AATT 775 779 . . 781 784 + DpnI GATC 782 782 . . 781 784 + BstKTI GATC 783 781 . . 781 784 + MboI GATC 780 784 . . 784 787 + AciI CCGC 784 786 . . 781 785 - BinI* GGATC 775 776 . . 808 813 + BsaAI YACGTR 810 810 . . 809 812 + TaiI ACGT 812 808 . . 809 812 + SetI ASST 812 808 . . 809 812 + MaeII ACGT 809 811 . . 814 818 + TfiI GAWTC 814 817 . . 814 818 + HinfI GANTC 814 817 . . 822 827 + SpeI ACTAGT 822 826 . . 823 826 + MaeI CTAG 823 825 . . 829 834 + AsuII TTCGAA 830 832 . . 830 833 + TaqI TCGA 830 832 . . 835 845 + MwoI GCNNNNNNNGC 841 838 . . 838 842 + DdeI CTNAG 838 841 . . 844 849 + Cac8I GCNNGC 846 846 . . 847 850 + SetI ASST 850 846 . . 847 850 + CviJI RGCY 848 848 . . 847 850 + AluI AGCT 848 848 . . 854 857 + Csp6I GTAC 854 856 . . 854 857 + RsaI GTAC 855 855 . . 863 866 + MnlI CCTC 873 872 . . 876 886 + FalI AAGNNNNNCTT 867 862 899 894 882 885 + CviJI RGCY 883 883 . . 876 886 - FalI AAGNNNNNCTT 899 894 867 862 889 899 + Hin4I GAYNNNNNVTC 880 875 912 907 889 899 - Hin4I GAYNNNNNVTC 912 907 880 875 902 905 + TspEI AATT 901 905 . . 917 920 + DpnI GATC 918 918 . . 917 920 + BstKTI GATC 919 917 . . 917 920 + MboI GATC 916 920 . . 922 925 + MaeI CTAG 922 924 . . 928 932 - BsmAI GTCTC 922 926 . . 928 933 - Eco31I GGTCTC 922 926 . . 942 946 + TfiI GAWTC 942 945 . . 942 946 + HinfI GANTC 942 945 . . 952 956 + TfiI GAWTC 952 955 . . 952 956 + HinfI GANTC 952 955 . . 960 963 + CviJI RGCY 961 961 . . 962 965 + MnlI CCTC 972 971 . . 969 974 + BclI TGATCA 969 973 . . 970 973 + DpnI GATC 971 971 . . 970 973 + BstKTI GATC 972 970 . . 970 973 + MboI GATC 969 973 . . 985 989 + BlsI GCNGC 987 986 . . 985 989 + BisI GCNGC 986 987 . . 985 989 + TseI GCWGC 985 988 . . 982 986 - SfaNI GCATC 972 976 . . 985 989 - BbvI GCAGC 972 976 . . 991 995 - BccI CCATC 985 986 . . 1000 1010 + BsaXI ACNNNNNCTCC 990 987 1020 1017 1002 1006 + TauI GCSGC 1005 1002 . . 1002 1006 + BlsI GCNGC 1004 1003 . . 1002 1006 + BisI GCNGC 1003 1004 . . 1003 1006 + AciI CCGC 1003 1005 . . 1006 1009 + MnlI CCTC 1016 1015 . . 1020 1023 + TspEI AATT 1019 1023 . . 1027 1031 + HgaI GACGC 1036 1041 . . 1030 1034 + BlsI GCNGC 1032 1031 . . 1030 1034 + BisI GCNGC 1031 1032 . . 1030 1034 + TseI GCWGC 1030 1033 . . 1032 1035 + CviRI* TGCA 1033 1033 . . 1034 1039 + MfeI CAATTG 1034 1038 . . 1030 1034 - BbvI GCAGC 1017 1021 . . 1035 1038 + TspEI AATT 1034 1038 . . 1048 1052 + Hpy188I TCNGA 1050 1049 . . 1052 1056 + Tsp4CI* ACNGT 1054 1053 . . 1062 1065 + SetI ASST 1065 1061 . . 1063 1067 + HphI GGTGA 1075 1074 . . 1066 1070 - Hin4II* CCTTC 1060 1059 . . 1071 1077 + TspRI CASTGNN 1077 1068 . . 1079 1083 + Tsp4CI* ACNGT 1081 1080 . . 1104 1107 + SetI ASST 1107 1103 . . 1104 1107 + CviJI RGCY 1105 1105 . . 1104 1107 + AluI AGCT 1105 1105 . . 1112 1115 + MaeI CTAG 1112 1114 . . 1117 1120 + CviRI* TGCA 1118 1118 . . 1126 1130 + MboII GAAGA 1138 1137 . . 1126 1131 - Ksp632I* CTCTTC 1121 1124 . . 1132 1136 + TfiI GAWTC 1132 1135 . . 1129 1132 - MnlI CCTC 1122 1121 . . 1132 1136 + HinfI GANTC 1132 1135 . . 1149 1154 + DsaI* CCRYGG 1149 1153 . . 1149 1154 + SecI* CCNNGG 1149 1153 . . 1150 1161 + TstI CACNNNNNNTCC 1141 1136 1173 1168 1151 1155 + Tsp4CI* ACNGT 1153 1152 . . 1170 1173 + CviJI RGCY 1171 1171 . . 1175 1179 + Hpy188I TCNGA 1177 1176 . . 1189 1194 + AclI AACGTT 1190 1192 . . 1190 1193 + TaiI ACGT 1193 1189 . . 1190 1193 + SetI ASST 1193 1189 . . 1190 1193 + MaeII ACGT 1190 1192 . . 1213 1217 + BccI CCATC 1221 1222 . . 1214 1218 - FokI GGATG 1200 1204 . . 1214 1218 - BseGI GGATG 1213 1211 . . 1224 1227 + SetI ASST 1227 1223 . . 1230 1233 + CviRI* TGCA 1231 1231 . . 1240 1243 + SetI ASST 1243 1239 . . 1251 1254 + TspEI AATT 1250 1254 . . 1276 1281 + ClaI ATCGAT 1277 1279 . . 1277 1280 + TaqI TCGA 1277 1279 . . 1279 1282 + DpnI GATC 1280 1280 . . 1279 1282 + BstKTI GATC 1281 1279 . . 1279 1282 + MboI GATC 1278 1282 . . 1290 1300 + FalI AAGNNNNNCTT 1281 1276 1313 1308 1292 1295 + Csp6I GTAC 1292 1294 . . 1292 1295 + RsaI GTAC 1293 1293 . . 1294 1297 + SetI ASST 1297 1293 . . 1295 1298 + MnlI CCTC 1305 1304 . . 1296 1301 + Ksp632I* CTCTTC 1302 1305 . . 1290 1300 - FalI AAGNNNNNCTT 1313 1308 1281 1276 1297 1301 - MboII GAAGA 1289 1288 . . 1301 1306 + EcoP15I CAGCAG 1331 1333 . . 1298 1303 - Eco57MI CTGRAG 1283 1281 . . 1298 1303 - Eco57I CTGAAG 1283 1281 . . 1306 1311 + BciVI GTATCC 1317 1316 . . 1315 1318 + TaqI TCGA 1315 1317 . . 1317 1327 + Hin4I GAYNNNNNVTC 1308 1303 1340 1335 1317 1327 - Hin4I GAYNNNNNVTC 1340 1335 1308 1303 1340 1343 + AciI CCGC 1340 1342 . . 1339 1344 - EciI GGCGGA 1329 1327 . . 1354 1358 + Hpy188I TCNGA 1356 1355 . . 1355 1360 + SecI* CCNNGG 1355 1359 . . 1359 1362 + CviJI RGCY 1360 1360 . . 1357 1360 - MnlI CCTC 1350 1349 . . 1363 1367 + Hpy188I TCNGA 1365 1364 . . 1371 1374 + CviRI* TGCA 1372 1372 . . 1378 1388 + BsaXI ACNNNNNCTCC 1368 1365 1398 1395 1380 1385 + NaeI GCCGGC 1382 1382 . . 1380 1385 + MroNI GCCGGC 1380 1384 . . 1380 1385 + Cac8I GCNNGC 1382 1382 . . 1380 1385 + Cfr10I RCCGGY 1380 1384 . . 1381 1384 + HpaII CCGG 1381 1383 . . 1383 1386 + CviJI RGCY 1384 1384 . . 1383 1388 + NlaIV GGNNCC 1385 1385 . . 1390 1393 + MseI TTAA 1390 1392 . . 1395 1400 + Hpy178III* TCNNGA 1396 1398 . . 1426 1429 - MnlI CCTC 1419 1418 . . 1441 1445 + HgaI GACGC 1450 1455 . . 1451 1454 + CviJI RGCY 1452 1452 . . 1456 1460 + TfiI GAWTC 1456 1459 . . 1456 1460 + HinfI GANTC 1456 1459 . . 1473 1478 + TsoI TARCCA 1489 1487 . . 1479 1484 + XhoII RGATCY 1479 1483 . . 1479 1484 + BglII AGATCT 1479 1483 . . 1480 1483 + DpnI GATC 1481 1481 . . 1480 1483 + BstKTI GATC 1482 1480 . . 1480 1483 + MboI GATC 1479 1483 . . 1483 1486 + MaeI CTAG 1483 1485 . . 1485 1488 + SetI ASST 1488 1484 . . 1485 1488 + CviJI RGCY 1486 1486 . . 1485 1488 + AluI AGCT 1486 1486 . . 1490 1493 + MseI TTAA 1490 1492 . . 1495 1499 - SfaNI GCATC 1485 1489 . . 1503 1507 + DdeI CTNAG 1503 1506 . . 1514 1518 + PleI GAGTC 1522 1523 . . 1514 1518 + MlyI GAGTC 1523 1523 . . 1514 1518 + HinfI GANTC 1514 1517 . . 1518 1521 + FatI CATG 1517 1521 . . 1518 1521 + NlaIII CATG 1521 1517 . . 1518 1521 + CviAII CATG 1518 1520 . . 1539 1542 + SetI ASST 1542 1538 . . 1556 1559 + TaqI TCGA 1556 1558 . . 1558 1568 + Hin4I GAYNNNNNVTC 1549 1544 1581 1576 1558 1568 + Eam1105I GACNNNNNGTC 1563 1562 . . 1560 1563 + FatI CATG 1559 1563 . . 1560 1563 + NlaIII CATG 1563 1559 . . 1560 1563 + CviAII CATG 1560 1562 . . 1564 1567 + SetI ASST 1567 1563 . . 1566 1570 + Tsp45I GTSAC 1565 1570 . . 1563 1566 - MnlI CCTC 1556 1555 . . 1566 1570 + MaeIII GTNAC 1565 1570 . . 1558 1568 - Hin4I GAYNNNNNVTC 1581 1576 1549 1544 1569 1574 + NspI RCATGY 1573 1569 . . 1570 1573 + FatI CATG 1569 1573 . . 1570 1573 + NlaIII CATG 1573 1569 . . 1570 1573 + CviAII CATG 1570 1572 . . 1573 1577 - BceAI ACGGC 1558 1560 . . 1579 1582 + SetI ASST 1582 1578 . . 1585 1590 + HindIII AAGCTT 1585 1589 . . 1586 1589 + SetI ASST 1589 1585 . . 1586 1589 + CviJI RGCY 1587 1587 . . 1586 1589 + AluI AGCT 1587 1587 . . 1595 1600 + Ksp632I* CTCTTC 1601 1604 . . 1596 1600 - MboII GAAGA 1588 1587 . . 1624 1627 + DpnI GATC 1625 1625 . . 1624 1627 + BstKTI GATC 1626 1624 . . 1624 1627 + MboI GATC 1623 1627 . . 1634 1637 + TaiI ACGT 1637 1633 . . 1634 1637 + SetI ASST 1637 1633 . . 1634 1637 + MaeII ACGT 1634 1636 . . 1663 1667 + BssKI CCNGG 1662 1667 . . 1663 1667 + BseBI CCWGG 1664 1665 . . 1663 1667 + ScrFI CCNGG 1664 1665 . . 1663 1667 + EcoRII CCWGG 1662 1667 . . 1671 1681 - BsaXI ACNNNNNCTCC 1693 1690 1663 1660 1671 1682 - TstI CACNNNNNNTCC 1695 1690 1663 1658 1687 1692 + ClaI ATCGAT 1688 1690 . . 1688 1691 + TaqI TCGA 1688 1690 . . 1690 1694 + TfiI GAWTC 1690 1693 . . 1690 1694 + HinfI GANTC 1690 1693 . . 1695 1698 + TspEI AATT 1694 1698 . . 1703 1708 + SspI AATATT 1705 1705 . . 1710 1713 + TaiI ACGT 1713 1709 . . 1710 1713 + SetI ASST 1713 1709 . . 1710 1713 + MaeII ACGT 1710 1712 . . 1728 1733 + BsmI GAATGC 1734 1732 . . 1732 1736 + BlsI GCNGC 1734 1733 . . 1732 1736 + BisI GCNGC 1733 1734 . . 1732 1736 + TseI GCWGC 1732 1735 . . 1736 1739 + MnlI CCTC 1746 1745 . . 1732 1736 - BbvI GCAGC 1719 1723 . . 1741 1744 + MnlI CCTC 1751 1750 . . 1744 1747 + MaeI CTAG 1744 1746 . . 1746 1749 + SetI ASST 1749 1745 . . 1746 1749 + CviJI RGCY 1747 1747 . . 1746 1749 + AluI AGCT 1747 1747 . . 1748 1753 - Eco57MI CTGRAG 1733 1731 . . 1748 1753 - GsuI CTGGAG 1733 1731 . . 1750 1754 - BsrI ACTGG 1750 1748 . . 1757 1760 + TaqI TCGA 1757 1759 . . 1760 1763 + SetI ASST 1763 1759 . . 1759 1762 - MnlI CCTC 1752 1751 . . 1762 1766 + MaeIII GTNAC 1761 1766 . . 1773 1776 + TspEI AATT 1772 1776 . . 1781 1784 + CviJI RGCY 1782 1782 . . 1780 1785 - TsoI TARCCA 1770 1768 . . 1795 1798 + TspEI AATT 1794 1798 . . 1811 1816 + Hpy178III* TCNNGA 1812 1814 . . 1815 1819 - Hin4II* CCTTC 1809 1808 . . 1820 1825 + ApoI RAATTY 1820 1824 . . 1821 1824 + TspEI AATT 1820 1824 . . 1825 1829 - BsrI ACTGG 1825 1823 . . 1834 1837 + MseI TTAA 1834 1836 . . 1858 1862 + Hpy188I TCNGA 1860 1859 . . 1862 1865 + TspEI AATT 1861 1865 . . 1864 1867 + MseI TTAA 1864 1866 . . 1865 1870 + TsoI TARCCA 1881 1879 . . 1876 1879 + SetI ASST 1879 1875 . . 1877 1880 + MnlI CCTC 1887 1886 . . 1900 1903 + MseI TTAA 1900 1902 . . 1916 1921 + ApoI RAATTY 1916 1920 . . 1917 1920 + TspEI AATT 1916 1920 . . 1922 1926 + MaeIII GTNAC 1921 1926 . . 1945 1950 + ApoI RAATTY 1945 1949 . . 1946 1949 + TspEI AATT 1945 1949 . . 1957 1960 + TspEI AATT 1956 1960 . . 1967 1971 + MaeIII GTNAC 1966 1971 . . 1971 1976 + SecI* CCNNGG 1971 1975 . . 1972 1976 + BssKI CCNGG 1971 1976 . . 1972 1976 + BseBI CCWGG 1973 1974 . . 1972 1976 + ScrFI CCNGG 1973 1974 . . 1972 1976 + EcoRII CCWGG 1971 1976 . . 1976 1981 + BsmI GAATGC 1982 1980 . . 1999 2003 + Tsp4CI* ACNGT 2001 2000 . . 2016 2026 + FalI AAGNNNNNCTT 2007 2002 2039 2034 2022 2025 + CviJI RGCY 2023 2023 . . 2026 2029 + MseI TTAA 2026 2028 . . 2016 2026 - FalI AAGNNNNNCTT 2039 2034 2007 2002 2030 2033 + MaeI CTAG 2030 2032 . . 2040 2043 + TspEI AATT 2039 2043 . . 2045 2049 + DdeI CTNAG 2045 2048 . . 2045 2049 + BspCNI CTCAG 2058 2056 . . 2045 2049 + BseMII CTCAG 2059 2057 . . 2046 2050 + Hpy188I TCNGA 2048 2047 . . 2049 2053 + HinfI GANTC 2049 2052 . . 2049 2053 - PleI GAGTC 2043 2044 . . 2049 2053 - MlyI GAGTC 2043 2043 . . 2057 2060 + DpnI GATC 2058 2058 . . 2057 2060 + BstKTI GATC 2059 2057 . . 2057 2060 + MboI GATC 2056 2060 . . 2069 2073 + TspDTI ATGAA 2084 2082 . . 2091 2096 + Hpy166II GTNNAC 2093 2093 . . 2095 2098 + SetI ASST 2098 2094 . . 2101 2104 + TspEI AATT 2100 2104 . . 2110 2115 + MfeI CAATTG 2110 2114 . . 2111 2114 + TspEI AATT 2110 2114 . . 2131 2136 + MmeI TCCRAC 2156 2154 . . 2128 2132 - MboII GAAGA 2120 2119 . . 2140 2144 - MboII GAAGA 2132 2131 . . 2144 2147 + MaeI CTAG 2144 2146 . . 2139 2144 - BbvII* GAAGAC 2132 2136 . . 2148 2153 + Hpy178III* TCNNGA 2149 2151 . . 2149 2159 + BsiYI* CCNNNNNNNGG 2155 2152 . . 2151 2162 + BdaI TGANNNNNNTCA 2140 2138 2174 2172 2151 2162 - BdaI TGANNNNNNTCA 2174 2172 2140 2138 2167 2170 + TspEI AATT 2166 2170 . . 2205 2209 + Tsp45I GTSAC 2204 2209 . . 2205 2209 + MaeIII GTNAC 2204 2209 . . 2212 2215 + TspEI AATT 2211 2215 . . 2215 2218 + CviRI* TGCA 2216 2216 . . 2238 2243 + SmlI CTYRAG 2238 2242 . . 2238 2243 + AflII CTTAAG 2238 2242 . . 2239 2242 + MseI TTAA 2239 2241 . . 2242 2245 + SetI ASST 2245 2241 . . 2242 2245 + CviJI RGCY 2243 2243 . . 2242 2245 + AluI AGCT 2243 2243 . . 2246 2249 + FatI CATG 2245 2249 . . 2246 2249 + NlaIII CATG 2249 2245 . . 2246 2249 + CviAII CATG 2246 2248 . . 2249 2254 + SduI GDGCHC 2253 2249 . . 2249 2254 + HgiJII* GRGCYC 2253 2249 . . 2250 2253 + CviJI RGCY 2251 2251 . . 2251 2261 + MwoI GCNNNNNNNGC 2257 2254 . . 2258 2263 + HindIII AAGCTT 2258 2262 . . 2259 2262 + SetI ASST 2262 2258 . . 2259 2262 + CviJI RGCY 2260 2260 . . 2259 2262 + AluI AGCT 2260 2260 . . 2262 2267 + AhaIII* TTTAAA 2264 2264 . . 2263 2266 + MseI TTAA 2263 2265 . . 2280 2286 + TspRI CASTGNN 2286 2277 . . 2280 2285 - BtsI GCAGTG 2279 2277 . . 2291 2294 + SetI ASST 2294 2290 . . 2311 2315 - MboII GAAGA 2303 2302 . . 2316 2319 + SetI ASST 2319 2315 . . 2316 2319 + CviJI RGCY 2317 2317 . . 2316 2319 + AluI AGCT 2317 2317 . . 2312 2317 - Eco57MI CTGRAG 2297 2295 . . 2312 2317 - Eco57I CTGAAG 2297 2295 . . 2322 2327 + Hpy178III* TCNNGA 2323 2325 . . 2326 2330 + TfiI GAWTC 2326 2329 . . 2326 2330 + HinfI GANTC 2326 2329 . . 2329 2334 + XbaI TCTAGA 2329 2333 . . 2329 2334 + Hpy178III* TCNNGA 2330 2332 . . 2330 2333 + MaeI CTAG 2330 2332 . . 2333 2336 + DpnI GATC 2334 2334 . . 2333 2336 + BstKTI GATC 2335 2333 . . 2333 2336 + MboI GATC 2332 2336 . . 2345 2349 + MaeIII GTNAC 2344 2349 . . 2349 2353 + Hpy99I CGWCG 2353 2348 . . 2350 2360 + Hin4I GAYNNNNNVTC 2341 2336 2373 2368 2350 2360 + Eam1105I GACNNNNNGTC 2355 2354 . . 2351 2354 + TaiI ACGT 2354 2350 . . 2351 2354 + SetI ASST 2354 2350 . . 2351 2354 + MaeII ACGT 2351 2353 . . 2356 2359 + SetI ASST 2359 2355 . . 2350 2360 - Hin4I GAYNNNNNVTC 2373 2368 2341 2336 2359 2363 - MboII GAAGA 2351 2350 . . 2358 2363 - BbvII* GAAGAC 2351 2355 . . 2396 2401 + NdeI CATATG 2397 2399 . . 2399 2403 + TspDTI ATGAA 2414 2412 . . 2401 2405 + MboII GAAGA 2413 2412 . . 2401 2406 + BbvII* GAAGAC 2408 2412 . . 2407 2411 + MboII GAAGA 2419 2418 . . 2417 2420 + DpnI GATC 2418 2418 . . 2417 2420 + BstKTI GATC 2419 2417 . . 2417 2420 + MboI GATC 2416 2420 . . 2440 2444 + HgaI GACGC 2449 2454 . . 2459 2462 + TspEI AATT 2458 2462 . . 2453 2464 - CspCI CAANNNNNGTGG 2477 2475 2442 2440 2466 2471 + ApoI RAATTY 2466 2470 . . 2467 2470 + TspEI AATT 2466 2470 . . 2476 2480 + DdeI CTNAG 2476 2479 . . 2494 2498 + Hpy188I TCNGA 2496 2495 . . 2532 2535 + SetI ASST 2535 2531 . . 2532 2535 + CviJI RGCY 2533 2533 . . 2532 2535 + AluI AGCT 2533 2533 . . 2541 2546 + TatI WGTACW 2541 2545 . . 2541 2546 + ScaI AGTACT 2543 2543 . . 2542 2545 + Csp6I GTAC 2542 2544 . . 2542 2545 + RsaI GTAC 2543 2543 . . 2539 2543 - BsrI ACTGG 2539 2537 . . 2549 2552 + MseI TTAA 2549 2551 . . 2572 2576 + TfiI GAWTC 2572 2575 . . 2572 2576 + HinfI GANTC 2572 2575 . . 2580 2583 + TspEI AATT 2579 2583 . . 2586 2591 - BsrDI GCAATG 2585 2583 . . 2596 2601 - TsoI TARCCA 2586 2584 . . 2613 2618 + StuI AGGCCT 2615 2615 . . 2614 2617 + CviJI RGCY 2615 2615 . . 2614 2617 + HaeIII GGCC 2615 2615 . . 2611 2615 - Hin4II* CCTTC 2605 2604 . . 2616 2619 + MnlI CCTC 2626 2625 . . 2616 2626 + BsiYI* CCNNNNNNNGG 2622 2619 . . 2618 2621 + TaqI TCGA 2618 2620 . . 2622 2625 + FatI CATG 2621 2625 . . 2622 2625 + NlaIII CATG 2625 2621 . . 2622 2625 + CviAII CATG 2622 2624 . . 2624 2629 + CfrI YGGCCR 2624 2628 . . 2625 2628 + CviJI RGCY 2626 2626 . . 2625 2628 + HaeIII GGCC 2626 2626 . . 2626 2630 + TauI GCSGC 2629 2626 . . 2626 2630 + BlsI GCNGC 2628 2627 . . 2626 2630 + BisI GCNGC 2627 2628 . . 2627 2632 + NspBII* CMGCKG 2629 2629 . . 2627 2637 + BsiYI* CCNNNNNNNGG 2633 2630 . . 2627 2630 + AciI CCGC 2627 2629 . . 2644 2647 + MaeI CTAG 2644 2646 . . 2650 2654 - Hin4II* CCTTC 2644 2643 . . 2654 2658 - BsmAI GTCTC 2648 2652 . . 2654 2659 - Esp3I CGTCTC 2648 2652 . . 2667 2672 - BtgZI GCGATG 2652 2656 . . 2678 2681 + CviRI* TGCA 2679 2679 . . 2679 2684 + Cac8I GCNNGC 2681 2681 . . 2706 2712 + TspRI CASTGNN 2712 2703 . . 2706 2715 + MslI CAYNNNNRTG 2710 2710 . . 2707 2711 + BsrI ACTGG 2712 2710 . . 2722 2727 + Hpy166II GTNNAC 2724 2724 . . 2722 2727 + HindII GTYRAC 2724 2724 . . 2725 2729 + HgaI GACGC 2734 2739 . . 2731 2734 + MseI TTAA 2731 2733 . . 2734 2737 + SetI ASST 2737 2733 . . 2753 2757 + Hpy188I TCNGA 2755 2754 . . 2761 2771 + EcoNI CCTNNNNNAGG 2765 2766 . . 2761 2765 + BssKI CCNGG 2760 2765 . . 2761 2765 + BseBI CCWGG 2762 2763 . . 2761 2765 + ScrFI CCNGG 2762 2763 . . 2761 2765 + EcoRII CCWGG 2760 2765 . . 2761 2771 + BsiYI* CCNNNNNNNGG 2767 2764 . . 2762 2767 + Eco57MI CTGRAG 2783 2781 . . 2762 2767 + GsuI CTGGAG 2783 2781 . . 2769 2772 + SetI ASST 2772 2768 . . 2767 2771 - Hin4II* CCTTC 2761 2760 . . 2778 2781 + TspEI AATT 2777 2781 . . 2786 2790 + TfiI GAWTC 2786 2789 . . 2786 2790 + HinfI GANTC 2786 2789 . . 2790 2793 + FatI CATG 2789 2793 . . 2790 2793 + NlaIII CATG 2793 2789 . . 2790 2793 + CviAII CATG 2790 2792 . . 2788 2792 - TspDTI ATGAA 2778 2776 . . 2797 2802 + ApoI RAATTY 2797 2801 . . 2798 2801 + TspEI AATT 2797 2801 . . 2803 2806 - AciI CCGC 2803 2805 . . 2815 2820 + Hpy166II GTNNAC 2817 2817 . . 2817 2821 + BmgT120I GGNCC 2818 2819 . . 2817 2821 + AvaII GGWCC 2817 2820 . . 2817 2821 + AsuI* GGNCC 2817 2820 . . 2826 2831 + SecI* CCNNGG 2826 2830 . . 2827 2831 + BssKI CCNGG 2826 2831 . . 2827 2831 + ScrFI CCNGG 2828 2829 . . 2827 2831 + CauII* CCSGG 2828 2829 . . 2828 2831 + HpaII CCGG 2828 2830 . . 2831 2835 + PleI GAGTC 2839 2840 . . 2831 2835 + MlyI GAGTC 2840 2840 . . 2831 2835 + HinfI GANTC 2831 2834 . . 2844 2849 + EciI GGCGGA 2860 2858 . . 2845 2855 + MwoI GCNNNNNNNGC 2851 2848 . . 2845 2848 - AciI CCGC 2845 2847 . . 2847 2852 - BciVI GTATCC 2841 2840 . . 2853 2856 + CviRI* TGCA 2854 2854 . . 2855 2860 + NdeI CATATG 2856 2858 . . 2876 2881 + BclI TGATCA 2876 2880 . . 2877 2880 + DpnI GATC 2878 2878 . . 2877 2880 + BstKTI GATC 2879 2877 . . 2877 2880 + MboI GATC 2876 2880 . . 2880 2883 + FatI CATG 2879 2883 . . 2880 2883 + NlaIII CATG 2883 2879 . . 2880 2883 + CviAII CATG 2880 2882 . . 2904 2908 + Tsp45I GTSAC 2903 2908 . . 2904 2908 + MaeIII GTNAC 2903 2908 . . 2955 2958 + SetI ASST 2958 2954 . . 2953 2957 - Hin4II* CCTTC 2947 2946 . . 2966 2970 + TspGWI ACGGA 2981 2979 . . 2969 2973 + MboII GAAGA 2981 2980 . . 2969 2974 - Ksp632I* CTCTTC 2964 2967 . . 2984 2988 - BsmAI GTCTC 2978 2982 . . 2997 3000 + SetI ASST 3000 2996 . . 2995 2999 - Hin4II* CCTTC 2989 2988 . . 2994 3006 - AloI GAACNNNNNNTCC 3018 3013 2986 2981 3012 3015 + TspEI AATT 3011 3015 . . 3023 3027 + TspDTI ATGAA 3038 3036 . . 3034 3037 + MseI TTAA 3034 3036 . . 3036 3039 + TspEI AATT 3035 3039 . . 3040 3044 + Hpy188I TCNGA 3042 3041 . . 3042 3053 + BdaI TGANNNNNNTCA 3031 3029 3065 3063 3048 3052 + SfaNI GCATC 3057 3061 . . 3042 3053 - BdaI TGANNNNNNTCA 3065 3063 3031 3029 3055 3060 + BsrDI GCAATG 3062 3060 . . 3066 3069 + SetI ASST 3069 3065 . . 3067 3071 + HphI GGTGA 3079 3078 . . 3070 3079 + BsaBI GATNNNNATC 3074 3074 . . 3083 3088 + MmeI TCCRAC 3108 3106 . . 3113 3116 + MseI TTAA 3113 3115 . . 3115 3118 + TspEI AATT 3114 3118 . . 3120 3125 + Hpy178III* TCNNGA 3121 3123 . . 3119 3124 - BsiI* CACGAG 3119 3123 . . 3136 3139 - MnlI CCTC 3129 3128 . . 3151 3155 + BsmAI GTCTC 3156 3160 . . 3161 3166 + ApoI RAATTY 3161 3165 . . 3162 3165 + TspEI AATT 3161 3165 . . 3165 3169 - TspDTI ATGAA 3155 3153 . . 3175 3179 + TspGWI ACGGA 3190 3188 . . 3179 3182 + TspEI AATT 3178 3182 . . 3188 3191 + MseI TTAA 3188 3190 . . 3192 3197 + ApoI RAATTY 3192 3196 . . 3193 3196 + TspEI AATT 3192 3196 . . 3195 3200 + AhaIII* TTTAAA 3197 3197 . . 3196 3199 + MseI TTAA 3196 3198 . . 3214 3217 + CviJI RGCY 3215 3215 . . 3214 3217 + HaeIII GGCC 3215 3215 . . 3214 3218 + BmgT120I GGNCC 3215 3216 . . 3214 3218 + AsuI* GGNCC 3214 3217 . . 3212 3215 - MnlI CCTC 3205 3204 . . 3220 3225 + ZraI GACGTC 3222 3222 . . 3220 3225 + AcyI GRCGYC 3221 3223 . . 3220 3225 + AatII GACGTC 3224 3220 . . 3221 3224 + TaiI ACGT 3224 3220 . . 3221 3224 + SetI ASST 3224 3220 . . 3221 3224 + MaeII ACGT 3221 3223 . . 3232 3236 + BlsI GCNGC 3234 3233 . . 3232 3236 + BisI GCNGC 3233 3234 . . 3232 3236 + TseI GCWGC 3232 3235 . . 3232 3236 - BbvI GCAGC 3219 3223 . . 3242 3245 + TaiI ACGT 3245 3241 . . 3242 3245 + SetI ASST 3245 3241 . . 3242 3245 + MaeII ACGT 3242 3244 . . 3249 3254 + NspI RCATGY 3253 3249 . . 3250 3253 + FatI CATG 3249 3253 . . 3250 3253 + NlaIII CATG 3253 3249 . . 3250 3253 + CviAII CATG 3250 3252 . . 3254 3257 + MseI TTAA 3254 3256 . . 3261 3264 + TspEI AATT 3260 3264 . . 3266 3269 + TaqI TCGA 3266 3268 . . 3266 3271 + Hpy178III* TCNNGA 3267 3269 . . 3268 3272 - BsmAI GTCTC 3262 3266 . . 3278 3282 + FokI GGATG 3291 3295 . . 3278 3282 + BseGI GGATG 3284 3282 . . 3292 3296 + BlsI GCNGC 3294 3293 . . 3292 3296 + BisI GCNGC 3293 3294 . . 3292 3296 + BbvI GCAGC 3304 3308 . . 3292 3296 + TseI GCWGC 3292 3295 . . 3294 3297 + SetI ASST 3297 3293 . . 3294 3297 + CviJI RGCY 3295 3295 . . 3294 3297 + AluI AGCT 3295 3295 . . 3306 3316 + BsiYI* CCNNNNNNNGG 3312 3309 . . 3311 3314 + MseI TTAA 3311 3313 . . 3355 3358 + CviJI RGCY 3356 3356 . . 3352 3356 - Hin4II* CCTTC 3346 3345 . . 3369 3372 + TspEI AATT 3368 3372 . . 3371 3374 + MseI TTAA 3371 3373 . . 3375 3378 + TspEI AATT 3374 3378 . . 3392 3396 + TfiI GAWTC 3392 3395 . . 3392 3396 + HinfI GANTC 3392 3395 . . 3400 3404 - SfaNI GCATC 3390 3394 . . 3404 3407 + MaeI CTAG 3404 3406 . . 3409 3412 + SetI ASST 3412 3408 . . 3410 3413 + MnlI CCTC 3420 3419 . . 3420 3423 + SetI ASST 3423 3419 . . 3434 3437 + TspEI AATT 3433 3437 . . 3440 3444 + MaeIII GTNAC 3439 3444 . . 3443 3446 + SetI ASST 3446 3442 . . 3444 3448 + Hin4II* CCTTC 3454 3453 . . 3452 3455 + SetI ASST 3455 3451 . . 3457 3461 + MboII GAAGA 3469 3468 . . 3470 3473 + MseI TTAA 3470 3472 . . 3483 3486 + CviRI* TGCA 3484 3484 . . 3484 3489 + BsrDI GCAATG 3491 3489 . . 3492 3495 + TspEI AATT 3491 3495 . . 3498 3503 + Hpy178III* TCNNGA 3499 3501 . . 3495 3499 - MboII GAAGA 3487 3486 . . 3507 3510 + FnuDII* CGCG 3508 3508 . . 3514 3518 + Hpy188I TCNGA 3516 3515 . . 3517 3521 - Hin4II* CCTTC 3511 3510 . . 3523 3526 + TspEI AATT 3522 3526 . . 3532 3535 - MnlI CCTC 3525 3524 . . 3535 3539 + BinI* GGATC 3543 3544 . . 3536 3539 + DpnI GATC 3537 3537 . . 3536 3539 + BstKTI GATC 3538 3536 . . 3536 3539 + MboI GATC 3535 3539 . . 3531 3541 - BsaXI ACNNNNNCTCC 3553 3550 3523 3520 3531 3542 - TstI CACNNNNNNTCC 3555 3550 3523 3518 3543 3547 - Hin4II* CCTTC 3537 3536 . . 3564 3568 + BsmAI GTCTC 3569 3573 . . 3583 3588 + MfeI CAATTG 3583 3587 . . 3584 3587 + TspEI AATT 3583 3587 . . 3588 3593 + EcoRV GATATC 3590 3590 . . 3597 3600 + SetI ASST 3600 3596 . . 3602 3605 + TaqI TCGA 3602 3604 . . 3613 3617 + Tsp4CI* ACNGT 3615 3614 . . 3615 3620 + NlaIV GGNNCC 3617 3617 . . 3618 3623 + Hpy178III* TCNNGA 3619 3621 . . 3608 3619 - PpiI GAACNNNNNCTC 3631 3626 3599 3594 3627 3630 + CviRI* TGCA 3628 3628 . . 3633 3636 + SetI ASST 3636 3632 . . 3633 3636 + CviJI RGCY 3634 3634 . . 3633 3636 + AluI AGCT 3634 3634 . . 3634 3644 + MwoI GCNNNNNNNGC 3640 3637 . . 3635 3640 - BsiI* CACGAG 3635 3639 . . 3642 3645 + CviRI* TGCA 3643 3643 . . 3650 3653 + MaeI CTAG 3650 3652 . . 3664 3668 + TfiI GAWTC 3664 3667 . . 3664 3668 + HinfI GANTC 3664 3667 . . 3670 3675 + Hpy178III* TCNNGA 3671 3673 . . 3673 3678 + XhoII RGATCY 3673 3677 . . 3674 3677 + DpnI GATC 3675 3675 . . 3674 3677 + BstKTI GATC 3676 3674 . . 3674 3677 + MboI GATC 3673 3677 . . 3674 3678 - BinI* GGATC 3668 3669 . . 3711 3715 - Hin4II* CCTTC 3705 3704 . . 3722 3733 + BstXI CCANNNNNNTGG 3729 3725 . . 3728 3731 + TspEI AATT 3727 3731 . . 3732 3735 + CviJI RGCY 3733 3733 . . 3734 3739 + SmlI CTYRAG 3734 3738 . . 3735 3740 + Hpy178III* TCNNGA 3736 3738 . . 3734 3739 - Bce83I* CTTGAG 3719 3717 . . 3757 3760 + SetI ASST 3760 3756 . . 3763 3767 - HphI GGTGA 3755 3754 . . 3778 3783 + ApoI RAATTY 3778 3782 . . 3779 3782 + TspEI AATT 3778 3782 . . 3781 3785 - TspDTI ATGAA 3771 3769 . . 3788 3791 + MnlI CCTC 3798 3797 . . 3792 3798 + TspRI CASTGNN 3798 3789 . . 3789 3794 - Eco57MI CTGRAG 3774 3772 . . 3789 3794 - GsuI CTGGAG 3774 3772 . . 3791 3795 - BsrI ACTGG 3791 3789 . . 3797 3800 + TspEI AATT 3796 3800 . . 3810 3814 - BccI CCATC 3804 3805 . . 3835 3839 + Hpy188I TCNGA 3837 3836 . . 3846 3849 + SetI ASST 3849 3845 . . 3872 3877 + HindIII AAGCTT 3872 3876 . . 3873 3876 + SetI ASST 3876 3872 . . 3873 3876 + CviJI RGCY 3874 3874 . . 3873 3876 + AluI AGCT 3874 3874 . . 3874 3884 + MwoI GCNNNNNNNGC 3880 3877 . . 3877 3880 + MseI TTAA 3877 3879 . . 3880 3890 + BaeI ACNNNNGTAYC 3869 3864 3902 3897 3881 3886 + NspBII* CMGCKG 3883 3883 . . 3881 3884 + AciI CCGC 3881 3883 . . 3895 3899 + MboII GAAGA 3907 3906 . . 3900 3903 + TspEI AATT 3899 3903 . . 3904 3908 + TfiI GAWTC 3904 3907 . . 3904 3908 + HinfI GANTC 3904 3907 . . 3910 3914 + Hpy188I TCNGA 3912 3911 . . 3932 3941 + XmnI GAANNNNTTC 3936 3936 . . 3928 3941 - AjuI GAANNNNNNNTTGG 3953 3948 3921 3916 3944 3947 + CviRI* TGCA 3945 3945 . . 3956 3959 + TaqI TCGA 3956 3958 . . 3965 3969 + MaeIII GTNAC 3964 3969 . . 3978 3982 + BinI* GGATC 3986 3987 . . 3979 3982 + DpnI GATC 3980 3980 . . 3979 3982 + BstKTI GATC 3981 3979 . . 3979 3982 + MboI GATC 3978 3982 . . 3991 3994 + MaeI CTAG 3991 3993 . . 3999 4003 + HgaI GACGC 4008 4013 . . 3999 4004 + AcyI GRCGYC 4000 4002 . . 4002 4006 + TauI GCSGC 4005 4002 . . 4002 4006 + BlsI GCNGC 4004 4003 . . 4002 4006 + BisI GCNGC 4003 4004 . . 4003 4006 + AciI CCGC 4003 4005 . . 4011 4014 + CviJI RGCY 4012 4012 . . 4025 4030 + HindIII AAGCTT 4025 4029 . . 4026 4029 + SetI ASST 4029 4025 . . 4026 4029 + CviJI RGCY 4027 4027 . . 4026 4029 + AluI AGCT 4027 4027 . . 4056 4060 + DdeI CTNAG 4056 4059 . . 4078 4083 + ClaI ATCGAT 4079 4081 . . 4079 4082 + TaqI TCGA 4079 4081 . . 4081 4085 + TfiI GAWTC 4081 4084 . . 4081 4085 + HinfI GANTC 4081 4084 . . 4093 4098 + MfeI CAATTG 4093 4097 . . 4089 4093 - HgaI GACGC 4078 4083 . . 4094 4097 + TspEI AATT 4093 4097 . . 4120 4123 - MnlI CCTC 4113 4112 . . 4124 4127 + TspEI AATT 4123 4127 . . 4120 4130 - Hin4I GAYNNNNNVTC 4143 4138 4111 4106 4131 4134 + SetI ASST 4134 4130 . . 4138 4143 + ApoI RAATTY 4138 4142 . . 4139 4142 + TspEI AATT 4138 4142 . . 4147 4150 + FatI CATG 4146 4150 . . 4147 4150 + NlaIII CATG 4150 4146 . . 4147 4150 + CviAII CATG 4147 4149 . . 4155 4160 + AhaIII* TTTAAA 4157 4157 . . 4156 4159 + MseI TTAA 4156 4158 . . 4161 4164 + SetI ASST 4164 4160 . . 4174 4178 + Tsp4CI* ACNGT 4176 4175 . . 4194 4200 + TspRI CASTGNN 4200 4191 . . 4196 4201 + Eco57MI CTGRAG 4217 4215 . . 4196 4201 + Eco57I CTGAAG 4217 4215 . . 4203 4208 + Hpy178III* TCNNGA 4204 4206 . . 4234 4238 - TspDTI ATGAA 4224 4222 . . 4249 4253 + Hpy188I TCNGA 4251 4250 . . 4267 4270 + CviJI RGCY 4268 4268 . . 4274 4277 + CviJI RGCY 4275 4275 . . 4281 4286 + Hpy178III* TCNNGA 4282 4284 . . 4285 4289 + TfiI GAWTC 4285 4288 . . 4285 4289 + HinfI GANTC 4285 4288 . . 4300 4304 + BceAI ACGGC 4316 4318 . . 4302 4305 + CviJI RGCY 4303 4303 . . 4303 4308 + Cac8I GCNNGC 4305 4305 . . 4308 4313 + SmlI CTYRAG 4308 4312 . . 4308 4313 - Bce83I* CTTGAG 4293 4291 . . 4314 4320 + TspRI CASTGNN 4320 4311 . . 4318 4322 + TfiI GAWTC 4318 4321 . . 4318 4322 + HinfI GANTC 4318 4321 . . 4330 4333 + MseI TTAA 4330 4332 . . 4335 4339 + HgaI GACGC 4344 4349 . . 4339 4344 + MfeI CAATTG 4339 4343 . . 4340 4343 + TspEI AATT 4339 4343 . . 4346 4351 - TsoI TARCCA 4336 4334 . . 4352 4357 + BsrDI GCAATG 4359 4357 . . 4363 4367 + Tsp45I GTSAC 4362 4367 . . 4363 4367 + MaeIII GTNAC 4362 4367 . . 4375 4380 + AsuII TTCGAA 4376 4378 . . 4376 4379 + TaqI TCGA 4376 4378 . . 4382 4385 + TaiI ACGT 4385 4381 . . 4382 4385 + SetI ASST 4385 4381 . . 4382 4385 + MaeII ACGT 4382 4384 . . 4391 4396 + SmlI CTYRAG 4391 4395 . . 4392 4397 + Hpy178III* TCNNGA 4393 4395 . . 4396 4400 + MboII GAAGA 4408 4407 . . 4391 4396 - Bce83I* CTTGAG 4376 4374 . . 4399 4403 - SfaNI GCATC 4389 4393 . . 4408 4412 + Hpy188I TCNGA 4410 4409 . . 4409 4413 + DdeI CTNAG 4409 4412 . . 4409 4413 - BspCNI CTCAG 4401 4399 . . 4409 4413 - BseMII CTCAG 4400 4398 . . 4427 4431 + Hpy188I TCNGA 4429 4428 . . 4424 4428 - MboII GAAGA 4416 4415 . . 4423 4428 - BbvII* GAAGAC 4416 4420 . . 4425 4430 - Eco57MI CTGRAG 4410 4408 . . 4425 4430 - Eco57I CTGAAG 4410 4408 . . 4436 4440 - HphI GGTGA 4428 4427 . . 4451 4455 + TfiI GAWTC 4451 4454 . . 4451 4455 + HinfI GANTC 4451 4454 . . 4460 4463 + SetI ASST 4463 4459 . . 4460 4463 + CviJI RGCY 4461 4461 . . 4460 4463 + AluI AGCT 4461 4461 . . 4470 4475 + NspI RCATGY 4474 4470 . . 4471 4474 + FatI CATG 4470 4474 . . 4471 4474 + NlaIII CATG 4474 4470 . . 4471 4474 + CviAII CATG 4471 4473 . . 4480 4484 + PleI GAGTC 4488 4489 . . 4480 4484 + MlyI GAGTC 4489 4489 . . 4480 4484 + HinfI GANTC 4480 4483 . . 4482 4486 + BsmAI GTCTC 4487 4491 . . 4486 4490 + DdeI CTNAG 4486 4489 . . 4497 4501 + HinfI GANTC 4497 4500 . . 4499 4503 + DdeI CTNAG 4499 4502 . . 4499 4503 + BspCNI CTCAG 4512 4510 . . 4499 4503 + BseMII CTCAG 4513 4511 . . 4500 4504 + Hpy188I TCNGA 4502 4501 . . 4497 4501 - PleI GAGTC 4491 4492 . . 4497 4501 - MlyI GAGTC 4491 4491 . . 4505 4508 + TaiI ACGT 4508 4504 . . 4505 4508 + SetI ASST 4508 4504 . . 4505 4508 + MaeII ACGT 4505 4507 . . 4506 4510 + Hpy99I CGWCG 4510 4505 . . 4529 4533 + TspDTI ATGAA 4544 4542 . . 4541 4546 + Eco57MI CTGRAG 4562 4560 . . 4541 4546 + Eco57I CTGAAG 4562 4560 . . 4543 4547 + MboII GAAGA 4555 4554 . . 4546 4550 + MboII GAAGA 4558 4557 . . 4558 4567 + MslI CAYNNNNRTG 4562 4562 . . 4564 4568 - SfaNI GCATC 4554 4558 . . 4592 4598 + PfoI TCCNGGA 4592 4597 . . 4593 4597 + BssKI CCNGG 4592 4597 . . 4593 4597 + BseBI CCWGG 4594 4595 . . 4593 4597 + ScrFI CCNGG 4594 4595 . . 4593 4597 + EcoRII CCWGG 4592 4597 . . 4596 4600 + FokI GGATG 4609 4613 . . 4596 4600 + BseGI GGATG 4602 4600 . . 4604 4609 + NlaIV GGNNCC 4606 4606 . . 4596 4608 - AloI GAACNNNNNNTCC 4620 4615 4588 4583 4635 4638 + DpnI GATC 4636 4636 . . 4635 4638 + BstKTI GATC 4637 4635 . . 4635 4638 + MboI GATC 4634 4638 . . 4637 4642 + Hpy178III* TCNNGA 4638 4640 . . 4637 4642 + BspHI TCATGA 4637 4641 . . 4638 4641 + FatI CATG 4637 4641 . . 4638 4641 + NlaIII CATG 4641 4637 . . 4638 4641 + CviAII CATG 4638 4640 . . 4643 4648 + PvuII CAGCTG 4645 4645 . . 4643 4648 + NspBII* CMGCKG 4645 4645 . . 4644 4647 + SetI ASST 4647 4643 . . 4644 4647 + CviJI RGCY 4645 4645 . . 4644 4647 + AluI AGCT 4645 4645 . . 4646 4651 + Eco57MI CTGRAG 4667 4665 . . 4646 4651 + Eco57I CTGAAG 4667 4665 . . 4648 4652 + MboII GAAGA 4660 4659 . . 4648 4653 + BbvII* GAAGAC 4655 4659 . . 4653 4657 + DdeI CTNAG 4653 4656 . . 4664 4667 + DpnI GATC 4665 4665 . . 4664 4667 + BstKTI GATC 4666 4664 . . 4664 4667 + MboI GATC 4663 4667 . . 4667 4672 + BsaAI YACGTR 4669 4669 . . 4667 4672 + PmaCI CACGTG 4669 4669 . . 4668 4671 + TaiI ACGT 4671 4667 . . 4668 4671 + SetI ASST 4671 4667 . . 4668 4671 + MaeII ACGT 4668 4670 . . 4671 4674 + CviRI* TGCA 4672 4672 . . 4675 4678 + MseI TTAA 4675 4677 . . 4688 4691 + TaqI TCGA 4688 4690 . . 4693 4703 + MwoI GCNNNNNNNGC 4699 4696 . . 4693 4703 + BglI GCCNNNNNGGC 4699 4696 . . 4690 4694 - SfaNI GCATC 4680 4684 . . 4696 4699 + MaeI CTAG 4696 4698 . . 4703 4708 + NdeI CATATG 4704 4706 . . 4714 4719 + ApoI RAATTY 4714 4718 . . 4715 4718 + TspEI AATT 4714 4718 . . 4752 4756 + MaeIII GTNAC 4751 4756 . . 4762 4772 + BsiYI* CCNNNNNNNGG 4768 4765 . . 4781 4791 + BsiYI* CCNNNNNNNGG 4787 4784 . . 4781 4791 + PflMI CCANNNNNTGG 4787 4784 . . 4795 4800 + Hpy166II GTNNAC 4797 4797 . . 4795 4800 + HindII GTYRAC 4797 4797 . . 4802 4805 + TspEI AATT 4801 4805 . . 4812 4817 + Hpy178III* TCNNGA 4813 4815 . . 4812 4817 + BspHI TCATGA 4812 4816 . . 4813 4816 + FatI CATG 4812 4816 . . 4813 4816 + NlaIII CATG 4816 4812 . . 4813 4816 + CviAII CATG 4813 4815 . . 4822 4825 + MseI TTAA 4822 4824 . . 4832 4835 + CviJI RGCY 4833 4833 . . 4837 4840 + TaqI TCGA 4837 4839 . . 4843 4846 + TaiI ACGT 4846 4842 . . 4843 4846 + SetI ASST 4846 4842 . . 4843 4846 + MaeII ACGT 4843 4845 . . 4846 4849 + MseI TTAA 4846 4848 . . 4848 4851 + TspEI AATT 4847 4851 . . 4854 4857 + CviJI RGCY 4855 4855 . . 4857 4862 + SmlI CTYRAG 4857 4861 . . 4857 4862 + AflII CTTAAG 4857 4861 . . 4858 4861 + MseI TTAA 4858 4860 . . 4894 4898 + TfiI GAWTC 4894 4897 . . 4894 4898 + HinfI GANTC 4894 4897 . . 4907 4912 + BsmI GAATGC 4913 4911 . . 4913 4916 + TaqI TCGA 4913 4915 . . 4915 4919 - BccI CCATC 4909 4910 . . 4930 4935 - EcoP15I CAGCAG 4902 4904 . . 4936 4941 + BciVI GTATCC 4947 4946 . . 4950 4955 + Hpy178III* TCNNGA 4951 4953 . . 4970 4973 + MaeI CTAG 4970 4972 . . 4974 4977 + TspEI AATT 4973 4977 . . 4981 4986 + XhoII RGATCY 4981 4985 . . 4982 4985 + DpnI GATC 4983 4983 . . 4982 4985 + BstKTI GATC 4984 4982 . . 4982 4985 + MboI GATC 4981 4985 . . 4982 4986 - BinI* GGATC 4976 4977 . . 4988 4993 + Cac8I GCNNGC 4990 4990 . . 4991 4994 + CviRI* TGCA 4992 4992 . . 4996 5001 + MfeI CAATTG 4996 5000 . . 4997 5000 + TspEI AATT 4996 5000 . . 5006 5009 + CviRI* TGCA 5007 5007 . . 5012 5015 + TaiI ACGT 5015 5011 . . 5012 5015 + SetI ASST 5015 5011 . . 5012 5015 + MaeII ACGT 5012 5014 . . 5020 5025 + ApoI RAATTY 5020 5024 . . 5021 5024 + TspEI AATT 5020 5024 . . 5031 5034 + Csp6I GTAC 5031 5033 . . 5031 5034 + RsaI GTAC 5032 5032 . . 5040 5044 + TfiI GAWTC 5040 5043 . . 5040 5044 + HinfI GANTC 5040 5043 . . 5049 5052 + SetI ASST 5052 5048 . . 5050 5054 + HphI GGTGA 5062 5061 . . 5051 5055 + Tsp45I GTSAC 5050 5055 . . 5051 5055 + MaeIII GTNAC 5050 5055 . . 5054 5057 + TaiI ACGT 5057 5053 . . 5054 5057 + SetI ASST 5057 5053 . . 5054 5057 + MaeII ACGT 5054 5056 . . 5060 5063 + TaqI TCGA 5060 5062 . . 5066 5069 + TspEI AATT 5065 5069 . . 5077 5080 + MseI TTAA 5077 5079 . . 5078 5083 + TsoI TARCCA 5094 5092 . . 5082 5088 + TspRI CASTGNN 5088 5079 . . 5082 5087 - BtsI GCAGTG 5081 5079 . . 5090 5094 + TspDTI ATGAA 5105 5103 . . 5093 5096 + TspEI AATT 5092 5096 . . 5099 5102 + TaqI TCGA 5099 5101 . . 5101 5110 + BsaBI GATNNNNATC 5105 5105 . . 5107 5110 + DpnI GATC 5108 5108 . . 5107 5110 + BstKTI GATC 5109 5107 . . 5107 5110 + MboI GATC 5106 5110 . . 5107 5111 - BinI* GGATC 5101 5102 . . 5112 5116 + MaeIII GTNAC 5111 5116 . . 5109 5113 - TspGWI ACGGA 5099 5097 . . 5123 5127 + MboII GAAGA 5135 5134 . . 5145 5149 - HphI GGTGA 5137 5136 . . 5167 5170 + CviJI RGCY 5168 5168 . . 5173 5182 + MslI CAYNNNNRTG 5177 5177 . . 5175 5179 + DdeI CTNAG 5175 5178 . . 5175 5179 + BspCNI CTCAG 5188 5186 . . 5175 5179 + BseMII CTCAG 5189 5187 . . 5176 5180 + Hpy188I TCNGA 5178 5177 . . 5198 5202 - Hin4II* CCTTC 5192 5191 . . 5209 5213 + TfiI GAWTC 5209 5212 . . 5209 5213 + HinfI GANTC 5209 5212 . . 5220 5226 + TspRI CASTGNN 5226 5217 . . 5227 5231 + Tsp4CI* ACNGT 5229 5228 . . 5228 5233 + McrI* CGRYCG 5231 5229 . . 5224 5228 - BsmAI GTCTC 5218 5222 . . 5224 5229 - Esp3I CGTCTC 5218 5222 . . 5231 5234 + TaqI TCGA 5231 5233 . . 5239 5242 + Hin6I GCGC 5239 5241 . . 5239 5242 + GlaI GCGC 5240 5240 . . 5239 5242 + HhaI GCGC 5241 5239 . . 5241 5245 + SfaNI GCATC 5250 5254 . . 5244 5249 + Hpy178III* TCNNGA 5245 5247 . . 5248 5252 + MboII GAAGA 5260 5259 . . 5251 5255 + TfiI GAWTC 5251 5254 . . 5251 5255 + HinfI GANTC 5251 5254 . . 5260 5264 + MboII GAAGA 5272 5271 . . 5260 5265 + BbvII* GAAGAC 5267 5271 . . 5264 5267 + TaiI ACGT 5267 5263 . . 5264 5267 + SetI ASST 5267 5263 . . 5264 5267 + MaeII ACGT 5264 5266 . . 5272 5276 + HphI GGTGA 5284 5283 . . 5273 5277 + Tsp45I GTSAC 5272 5277 . . 5273 5277 + MaeIII GTNAC 5272 5277 . . 5277 5280 + FatI CATG 5276 5280 . . 5277 5280 + NlaIII CATG 5280 5276 . . 5277 5280 + CviAII CATG 5277 5279 . . 5298 5308 + MwoI GCNNNNNNNGC 5304 5301 . . 5303 5307 + DdeI CTNAG 5303 5306 . . 5305 5310 + HindIII AAGCTT 5305 5309 . . 5306 5309 + SetI ASST 5309 5305 . . 5306 5309 + CviJI RGCY 5307 5307 . . 5306 5309 + AluI AGCT 5307 5307 . . 5309 5312 + MseI TTAA 5309 5311 . . 5320 5323 + TspEI AATT 5319 5323 . . 5323 5327 - TspGWI ACGGA 5313 5311 . . 5328 5333 + MmeI TCCRAC 5353 5351 . . 5328 5332 + Hpy188I TCNGA 5330 5329 . . 5330 5334 + Hpy99I CGWCG 5334 5329 . . 5332 5336 + Tsp4CI* ACNGT 5334 5333 . . 5345 5348 + TspEI AATT 5344 5348 . . 5349 5353 + TfiI GAWTC 5349 5352 . . 5349 5353 + HinfI GANTC 5349 5352 . . 5357 5360 + CviJI RGCY 5358 5358 . . 5358 5363 + Cac8I GCNNGC 5360 5360 . . 5359 5363 + FauI CCCGC 5367 5369 . . 5360 5363 + AciI CCGC 5360 5362 . . 5363 5366 + MaeI CTAG 5363 5365 . . 5368 5372 + DdeI CTNAG 5368 5371 . . 5372 5377 + BciVI GTATCC 5383 5382 . . 5387 5391 + DdeI CTNAG 5387 5390 . . 5394 5397 + FatI CATG 5393 5397 . . 5394 5397 + NlaIII CATG 5397 5393 . . 5394 5397 + CviAII CATG 5394 5396 . . 5395 5399 + TspDTI ATGAA 5410 5408 . . 5411 5415 + MaeIII GTNAC 5410 5415 . . 5416 5420 + MboII GAAGA 5428 5427 . . 5423 5428 + SnaBI TACGTA 5425 5425 . . 5423 5428 + BsaAI YACGTR 5425 5425 . . 5424 5427 + TaiI ACGT 5427 5423 . . 5424 5427 + SetI ASST 5427 5423 . . 5424 5427 + MaeII ACGT 5424 5426 . . 5441 5446 + Hpy178III* TCNNGA 5442 5444 . . 5445 5450 + EcoRI GAATTC 5445 5449 . . 5445 5450 + ApoI RAATTY 5445 5449 . . 5446 5449 + TspEI AATT 5445 5449 . . 5474 5477 + SetI ASST 5477 5473 . . 5474 5477 + CviJI RGCY 5475 5475 . . 5474 5477 + AluI AGCT 5475 5475 . . 5489 5492 + SetI ASST 5492 5488 . . 5489 5492 + CviJI RGCY 5490 5490 . . 5489 5492 + AluI AGCT 5490 5490 . . 5490 5494 + BlsI GCNGC 5492 5491 . . 5490 5494 + BisI GCNGC 5491 5492 . . 5490 5494 + TseI GCWGC 5490 5493 . . 5490 5494 - BbvI GCAGC 5477 5481 . . 5501 5504 + TspEI AATT 5500 5504 . . 5507 5510 + TaqI TCGA 5507 5509 . . 5507 5512 + Hpy178III* TCNNGA 5508 5510 . . 5514 5525 + BcgI CGANNNNNNTGC 5503 5501 5537 5535 5515 5519 + MboII GAAGA 5527 5526 . . 5527 5533 + TspRI CASTGNN 5533 5524 . . 5528 5532 + Tsp4CI* ACNGT 5530 5529 . . 5531 5536 + BciVI GTATCC 5542 5541 . . 5535 5545 + BsiYI* CCNNNNNNNGG 5541 5538 . . 5547 5550 + CviJI RGCY 5548 5548 . . 5556 5559 + TspEI AATT 5555 5559 . . 5568 5573 - MmeI TCCRAC 5549 5547 . . 5579 5584 + SplI* CGTACG 5579 5583 . . 5580 5583 + Csp6I GTAC 5580 5582 . . 5580 5583 + RsaI GTAC 5581 5581 . . 5571 5581 - BsaXI ACNNNNNCTCC 5593 5590 5563 5560 5584 5589 + ApoI RAATTY 5584 5588 . . 5585 5588 + TspEI AATT 5584 5588 . . 5592 5597 + Hpy178III* TCNNGA 5593 5595 . . 5594 5598 + Tsp45I GTSAC 5593 5598 . . 5594 5598 + MaeIII GTNAC 5593 5598 . . 5591 5596 - BsiI* CACGAG 5591 5595 . . 5600 5603 + MseI TTAA 5600 5602 . . 5603 5607 + TspDTI ATGAA 5618 5616 . . 5605 5609 + MboII GAAGA 5617 5616 . . 5617 5620 + MseI TTAA 5617 5619 . . 5628 5633 + SpeI ACTAGT 5628 5632 . . 5629 5632 + MaeI CTAG 5629 5631 . . 5637 5640 + CviJI RGCY 5638 5638 . . 5635 5638 - MnlI CCTC 5628 5627 . . 5639 5644 - BsiI* CACGAG 5639 5643 . . 5663 5666 + SetI ASST 5666 5662 . . 5686 5690 + BlsI GCNGC 5688 5687 . . 5686 5690 + BisI GCNGC 5687 5688 . . 5686 5690 + TseI GCWGC 5686 5689 . . 5689 5693 + BlsI GCNGC 5691 5690 . . 5689 5693 + BisI GCNGC 5690 5691 . . 5689 5693 + TseI GCWGC 5689 5692 . . 5690 5695 + PstI CTGCAG 5694 5690 . . 5690 5695 + SfeI* CTRYAG 5690 5694 . . 5686 5690 - BbvI GCAGC 5673 5677 . . 5691 5694 + CviRI* TGCA 5692 5692 . . 5687 5692 - EcoP15I CAGCAG 5659 5661 . . 5689 5693 - BbvI GCAGC 5676 5680 . . 5724 5728 + Hpy188I TCNGA 5726 5725 . . 5739 5743 + MboII GAAGA 5751 5750 . . 5745 5748 + CviJI RGCY 5746 5746 . . 5742 5746 - Hin4II* CCTTC 5736 5735 . . 5752 5755 + MseI TTAA 5752 5754 . . 5763 5767 + Hpy188I TCNGA 5765 5764 . . 5765 5770 + BclI TGATCA 5765 5769 . . 5766 5769 + DpnI GATC 5767 5767 . . 5766 5769 + BstKTI GATC 5768 5766 . . 5766 5769 + MboI GATC 5765 5769 . . 5768 5772 - HphI GGTGA 5760 5759 . . 5776 5779 + CviRI* TGCA 5777 5777 . . 5777 5781 + SfaNI GCATC 5786 5790 . . 5781 5785 + BssKI CCNGG 5780 5785 . . 5781 5785 + BseBI CCWGG 5782 5783 . . 5781 5785 + ScrFI CCNGG 5782 5783 . . 5781 5785 + EcoRII CCWGG 5780 5785 . . 5778 5782 - FokI GGATG 5764 5768 . . 5778 5782 - BseGI GGATG 5777 5775 . . 5783 5786 + SetI ASST 5786 5782 . . 5777 5788 - BcgI CGANNNNNNTGC 5800 5798 5766 5764 5791 5794 - MnlI CCTC 5784 5783 . . 5808 5812 + FokI GGATG 5821 5825 . . 5808 5812 + BseGI GGATG 5814 5812 . . 5819 5824 + DsaI* CCRYGG 5819 5823 . . 5819 5824 + StyI CCWWGG 5819 5823 . . 5819 5824 + NcoI CCATGG 5819 5823 . . 5819 5824 + SecI* CCNNGG 5819 5823 . . 5820 5823 + FatI CATG 5819 5823 . . 5820 5823 + NlaIII CATG 5823 5819 . . 5820 5823 + CviAII CATG 5820 5822 . . 5826 5829 + TaiI ACGT 5829 5825 . . 5826 5829 + SetI ASST 5829 5825 . . 5826 5829 + MaeII ACGT 5826 5828 . . 5840 5844 + BssKI CCNGG 5839 5844 . . 5840 5844 + BseBI CCWGG 5841 5842 . . 5840 5844 + ScrFI CCNGG 5841 5842 . . 5840 5844 + EcoRII CCWGG 5839 5844 . . 5849 5855 + TspRI CASTGNN 5855 5846 . . 5862 5867 + SspI AATATT 5864 5864 . . 5871 5874 + SetI ASST 5874 5870 . . 5873 5877 + MaeIII GTNAC 5872 5877 . . 5881 5886 + ApoI RAATTY 5881 5885 . . 5882 5885 + TspEI AATT 5881 5885 . . 5891 5895 + TspDTI ATGAA 5906 5904 . . 5893 5897 + MboII GAAGA 5905 5904 . . 5893 5898 + BbvII* GAAGAC 5900 5904 . . 5903 5914 + DrdI GACNNNNNNGTC 5909 5907 . . 5909 5913 + Tsp4CI* ACNGT 5911 5910 . . 5917 5927 + MwoI GCNNNNNNNGC 5923 5920 . . 5931 5935 + HphI GGTGA 5943 5942 . . 5934 5938 + TfiI GAWTC 5934 5937 . . 5934 5938 + HinfI GANTC 5934 5937 . . 5940 5945 + SspI AATATT 5942 5942 . . 5958 5963 + MmeI TCCRAC 5983 5981 . . 5962 5966 + TspGWI ACGGA 5977 5975 . . 5973 5978 + NspI RCATGY 5977 5973 . . 5974 5977 + FatI CATG 5973 5977 . . 5974 5977 + NlaIII CATG 5977 5973 . . 5974 5977 + CviAII CATG 5974 5976 . . 5970 5974 - BsmAI GTCTC 5964 5968 . . 5987 5990 + TspEI AATT 5986 5990 . . 5992 5996 + DdeI CTNAG 5992 5995 . . 6004 6008 + HphI GGTGA 6016 6015 . . 6008 6011 + TspEI AATT 6007 6011 . . 6014 6017 + SetI ASST 6017 6013 . . 6027 6030 + MseI TTAA 6027 6029 . . #--------------------------------------- # # # AatII: # [GACGTC] # 2806 3224 # # AccI: # [GTMKAC] # 613 5417 # # AciI: # [CCGC] # 42 122 176 219 287 337 # 497 670 1036 1287 1357 # # AclI: # [AACGTT] # 1190 4840 # # AcyI: # [GRCGYC] # 779 2030 3221 # # AflII: # [CTTAAG] # 1173 2238 2619 # # AhaIII*: # [TTTAAA] # 704 933 960 1560 1873 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 673 2077 3216 # # AloI: # [GAACNNNNNNTCC] # 32 32 1410 1570 2986 # # AluI: # [AGCT] # 15 17 51 57 83 101 # 121 153 160 168 184 216 # 240 257 286 307 339 381 # 434 496 540 662 762 # # ApoI: # [RAATTY] # 9 12 29 31 40 96 # 139 149 297 306 331 360 # 364 425 521 576 586 714 # 1045 # # AsuI*: # [GGNCC] # 157 397 2660 2816 # # AsuII: # [TTCGAA] # 78 100 652 1654 3546 # # AvaII: # [GGWCC] # 2817 3213 # # BaeI: # [ACNNNNGTAYC] # 33 2128 3869 # # BbvI: # [GCAGC] # 3 45 85 196 354 702 # 972 1500 2173 # # BbvII*: # [GAAGAC] # 57 60 130 219 239 326 # 612 633 1746 2008 # # BccI: # [CCATC] # 34 180 236 240 531 1105 # 1121 2583 # # Bce83I*: # [CTTGAG] # 83 574 1654 3719 # # BceAI: # [ACGGC] # 194 1364 1714 2758 # # BcgI: # [CGANNNNNNTGC] # 34 34 34 229 230 583 # 4886 # # BciVI: # [GTATCC] # 159 436 488 1317 1524 2106 # # BclI: # [TGATCA] # 265 969 1907 2889 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 857 2140 2965 # # BglI: # [GCCNNNNNGGC] # 1331 4699 # # BglII: # [AGATCT] # 1479 4551 # # BinI*: # [GGATC] # 125 125 318 775 929 990 # 2768 # # BisI: # [GCNGC] # 3 17 28 60 196 340 # 606 702 710 894 986 1488 # # BlsI: # [GCNGC] # 3 17 28 60 196 339 # 606 702 710 894 987 1488 # # BmgT120I: # [GGNCC] # 158 397 2660 2815 # # BplI: # [GAGNNNNNCTC] # 0 0 32 412 5586 # # BsaAI: # [YACGTR] # 605 756 810 3859 # # BsaBI: # [GATNNNNATC] # 925 2031 3074 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 30 30 265 # 348 437 990 1830 2010 # # BseBI: # [CCWGG] # 59 189 309 789 1188 1664 # 1832 # # BseGI: # [GGATG] # 37 45 216 297 871 1175 # 1318 2071 # # BseMII: # [CTCAG] # 9 113 332 410 676 841 # 1308 2341 # # BseRI: # [GAGGAG] # 471 5559 # # BsiI*: # [CACGAG] # 48 391 516 1956 3119 # # BsiYI*: # [CCNNNNNNNGG] # 11 19 134 240 383 467 # 489 545 754 1456 1532 # # BslFI: # [GGGAC] # 343 5687 # # BsmAI: # [GTCTC] # 58 63 66 106 178 234 # 280 287 307 330 731 746 # 918 1726 # # BsmI: # [GAATGC] # 108 248 630 996 1117 2931 # # BspCNI: # [CTCAG] # 7 111 332 411 676 842 # 1308 2343 # # BspHI: # [TCATGA] # 175 1218 4637 # # BsrDI: # [GCAATG] # 429 477 868 1671 2585 # # BsrI: # [ACTGG] # 75 173 371 714 1079 1379 # 2239 # # BssKI: # [CCNGG] # 59 66 191 309 789 1188 # 1662 1766 # # BstKTI: # [GATC] # 29 53 84 125 136 138 # 144 144 201 262 276 305 # 309 318 433 460 639 656 # 659 659 # # BstXI: # [CCANNNNNNTGG] # 2301 3729 # # BtgZI: # [GCGATG] # 473 2179 3378 # # BtrI: # [CACGTC] # 348 5682 # # BtsI: # [GCAGTG] # 949 2279 2802 # # Cac8I: # [GCNNGC] # 171 294 370 381 536 670 # 685 1299 1624 # # CauII*: # [CCSGG] # 2828 3202 # # Cfr10I: # [RCCGGY] # 1380 4650 # # CfrI: # [YGGCCR] # 2624 3406 # # ClaI: # [ATCGAT] # 411 1277 1951 2391 # # Csp6I: # [GTAC] # 176 240 438 438 450 549 # 1250 2489 # # CspCI: # [CAANNNNNGTGG] # 35 2442 3553 # # CviAII: # [CATG] # 10 42 56 90 117 154 # 167 168 175 262 324 370 # 376 426 464 676 897 1256 # # CviJI: # [RGCY] # 7 8 9 11 15 15 # 22 24 28 34 35 35 # 38 51 51 57 58 61 # 66 68 78 80 82 83 # 90 99 101 103 108 117 # 121 138 139 141 144 158 # 160 183 184 188 189 216 # 220 241 241 269 278 284 # 313 589 # # CviRI*: # [TGCA] # 15 15 85 85 113 141 # 144 152 175 253 302 320 # 463 630 685 727 844 881 # # DdeI: # [CTNAG] # 13 14 19 38 65 77 # 101 128 154 318 353 405 # 431 522 542 605 665 1580 # # DpnI: # [GATC] # 29 53 84 125 136 138 # 143 144 201 263 276 305 # 309 318 433 460 639 656 # 659 659 # # DrdI: # [GACNNNNNNGTC] # 121 5909 # # DsaI*: # [CCRYGG] # 211 1149 4670 # # Eam1105I: # [GACNNNNNGTC] # 792 1563 3675 # # EciI: # [GGCGGA] # 1329 1531 3170 # # Eco31I: # [GGTCTC] # 922 5108 # # Eco57I: # [CTGAAG] # 59 105 152 193 1014 1224 # 1363 1920 # # Eco57MI: # [CTGRAG] # 59 105 152 193 393 443 # 450 486 564 831 991 1363 # # EcoNI: # [CCTNNNNNAGG] # 2765 3265 # # EcoP15I: # [CAGCAG] # 371 757 1331 3571 # # EcoRI: # [GAATTC] # 12 585 714 4719 # # EcoRII: # [CCWGG] # 59 191 309 789 1188 1662 # 1832 # # EcoRV: # [GATATC] # 2440 3590 # # Esp3I: # [CGTCTC] # 812 2570 2648 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 382 694 867 # 3991 # # FatI: # [CATG] # 10 42 57 90 117 154 # 167 168 175 261 324 370 # 376 426 464 676 897 1256 # # FauI: # [CCCGC] # 490 663 4877 # # FnuDII*: # [CGCG] # 2522 3508 # # FokI: # [GGATG] # 52 57 209 297 851 1155 # 1318 2091 # # GlaI: # [GCGC] # 790 5240 # # GsuI: # [CTGGAG] # 452 991 1050 1281 2256 # # HaeIII: # [GGCC] # 11 159 589 2456 2815 # # HgaI: # [GACGC] # 70 214 266 285 414 822 # 999 1274 1686 # # HgiJII*: # [GRGCYC] # 2253 3777 # # HhaI: # [GCGC] # 789 5241 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 16 32 32 32 32 32 # 32 32 209 382 396 418 # 760 1738 1887 # # Hin4II*: # [CCTTC] # 26 39 42 57 108 117 # 168 186 265 294 325 357 # 470 544 749 796 1487 # # Hin6I: # [GCGC] # 791 5239 # # HindII: # [GTYRAC] # 1233 2073 2724 # # HindIII: # [AAGCTT] # 153 673 725 1280 1585 1614 # # HinfI: # [GANTC] # 6 10 17 29 33 42 # 45 48 58 96 98 128 # 133 146 150 169 176 177 # 180 204 214 219 240 246 # 272 277 324 359 391 397 # 561 585 # # HpaII: # [CCGG] # 1381 1447 3202 # # HphI: # [GGTGA] # 14 73 75 138 147 148 # 183 213 476 576 634 673 # 676 2004 # # Hpy166II: # [GTNNAC] # 93 614 631 1233 1479 1980 # # Hpy178III*: # [TCNNGA] # 7 52 65 66 78 85 # 111 120 138 143 146 174 # 175 197 232 245 294 337 # 416 431 437 468 791 822 # # Hpy188I: # [TCNGA] # 6 9 12 18 19 39 # 54 54 73 75 127 152 # 159 179 188 236 259 265 # 287 310 321 339 360 396 # 448 474 495 676 # # Hpy99I: # [CGWCG] # 208 696 824 2145 2157 # # Ksp632I*: # [CTCTTC] # 181 299 533 588 1363 3066 # # MaeI: # [CTAG] # 99 114 186 190 246 261 # 266 274 286 314 341 371 # 393 401 705 760 823 # # MaeII: # [ACGT] # 21 42 76 121 123 160 # 163 169 175 204 210 341 # 347 381 402 444 641 870 # 1140 # # MaeIII: # [GTNAC] # 45 61 138 140 158 160 # 161 183 196 199 238 279 # 299 389 398 525 536 559 # 1366 # # MboI: # [GATC] # 29 53 84 125 136 138 # 141 144 201 265 276 305 # 309 318 433 460 639 656 # 659 659 # # MboII: # [GAAGA] # 3 3 3 3 3 3 # 3 3 3 3 3 3 # 6 6 6 6 6 6 # 6 8 9 9 12 12 # 12 18 21 24 36 44 # 48 48 60 62 62 63 # 82 90 93 99 102 125 # 125 134 139 151 154 156 # 171 299 420 475 488 501 # 506 532 562 # # McrI*: # [CGRYCG] # 799 5231 # # MfeI: # [CAATTG] # 246 510 657 1034 1034 1076 # 1473 # # MlyI: # [GAGTC] # 2 520 797 1523 1539 1649 # # MmeI: # [TCCRAC] # 47 196 434 671 952 1485 # 2245 # # MnlI: # [CCTC] # 1 5 9 42 44 45 # 69 76 83 99 105 106 # 135 137 156 156 183 190 # 215 246 256 273 315 327 # 503 739 1515 # # MroNI: # [GCCGGC] # 1380 4650 # # MseI: # [TTAA] # 3 8 12 16 17 24 # 24 30 36 51 57 58 # 60 75 79 99 100 110 # 126 135 147 174 182 213 # 219 232 275 279 286 291 # 303 344 345 407 593 620 # # MslI: # [CAYNNNNRTG] # 266 615 853 1852 2444 # # MwoI: # [GCNNNNNNNGC] # 99 107 240 594 605 619 # 742 789 819 1416 # # NaeI: # [GCCGGC] # 1382 4648 # # NcoI: # [CCATGG] # 211 5819 # # NdeI: # [CATATG] # 459 1326 1848 2397 # # NlaIII: # [CATG] # 10 42 53 90 117 154 # 167 168 175 265 324 370 # 376 426 464 676 897 1256 # # NlaIV: # [GGNNCC] # 989 1385 1424 2232 # # NspBII*: # [CMGCKG] # 762 1254 1385 2629 # # NspI: # [RCATGY] # 53 1221 1503 1573 1680 # # PflMI: # [CCANNNNNTGG] # 1243 4787 # # PfoI: # [TCCNGGA] # 1438 4592 # # PleI: # [GAGTC] # 3 521 796 1522 1539 1649 # # PmaCI: # [CACGTG] # 1361 4669 # # PpiI: # [GAACNNNNNCTC] # 32 32 646 2399 2921 # # PstI: # [CTGCAG] # 336 5694 # # PvuII: # [CAGCTG] # 1385 4645 # # RsaI: # [GTAC] # 177 240 438 438 449 549 # 1250 2489 # # ScaI: # [AGTACT] # 417 2126 3487 # # ScrFI: # [CCNGG] # 59 66 189 309 789 1188 # 1664 1766 # # SduI: # [GDGCHC] # 2253 3777 # # SecI*: # [CCNNGG] # 206 211 542 607 616 855 # 2993 # # SetI: # [ASST] # 5 5 7 9 11 13 # 14 15 15 16 17 21 # 23 24 25 25 27 30 # 32 34 35 35 36 36 # 37 38 42 42 42 43 # 45 45 48 49 50 51 # 52 54 54 69 74 76 # 78 78 80 83 86 89 # 105 115 116 118 120 121 # 123 124 139 143 145 147 # 153 153 155 169 174 175 # 176 186 191 202 210 215 # 219 221 # # SfaNI: # [GCATC] # 126 165 244 333 513 536 # 570 972 999 1572 # # SfeI*: # [CTRYAG] # 340 5690 # # SmlI: # [CTYRAG] # 83 466 574 1173 1496 2238 # # SnaBI: # [TACGTA] # 605 5425 # # SpeI: # [ACTAGT] # 402 822 4806 # # SplI*: # [CGTACG] # 451 5579 # # SspI: # [AATATT] # 78 88 1705 4159 # # StuI: # [AGGCCT] # 2615 3415 # # StyI: # [CCWWGG] # 211 607 5212 # # TaiI: # [ACGT] # 21 42 76 121 123 160 # 163 169 175 201 210 341 # 350 381 402 444 641 870 # 1140 # # TaqI: # [TCGA] # 19 38 39 69 76 78 # 81 123 132 132 147 149 # 241 276 297 312 336 354 # 447 523 648 652 861 # # TatI: # [WGTACW] # 415 2126 3489 # # TauI: # [GCSGC] # 1005 1376 1624 2025 # # TfiI: # [GAWTC] # 6 10 33 42 48 96 # 98 128 133 146 150 169 # 177 180 204 214 219 234 # 240 246 272 324 391 443 # 585 606 636 # # TseI: # [GCWGC] # 3 45 60 196 341 702 # 985 1500 2198 # # TsoI: # [TARCCA] # 111 281 705 758 936 1489 # 1750 # # Tsp45I: # [GTSAC] # 222 321 437 639 688 699 # 1459 1565 # # Tsp4CI*: # [ACNGT] # 27 72 105 119 196 381 # 561 848 1053 1054 1614 # # TspDTI: # [ATGAA] # 6 6 6 6 6 12 # 18 39 49 117 123 124 # 208 235 260 288 305 320 # 330 364 453 561 616 1578 # # TspEI: # [AATT] # 6 8 9 10 11 12 # 14 15 15 17 18 20 # 21 22 23 23 24 24 # 25 26 27 27 28 29 # 29 30 31 40 41 45 # 45 46 47 51 55 55 # 55 56 58 59 61 61 # 68 78 79 83 87 101 # 103 105 108 113 118 126 # 126 144 194 198 201 214 # 216 227 247 297 375 444 # 659 # # TspGWI: # [ACGGA] # 23 53 74 156 209 214 # 664 1909 2728 # # TspRI: # [CASTGNN] # 120 138 175 307 322 402 # 426 768 1077 1086 1209 # # TstI: # [CACNNNNNNTCC] # 32 32 32 490 1141 1828 # 2475 # # XbaI: # [TCTAGA] # 2329 3701 # # XhoII: # [RGATCY] # 1049 1308 1479 2194 # # XmnI: # [GAANNNNTTC] # 2094 3936 # # ZraI: # [GACGTC] # 2808 3222 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 6030 # Reported_sequences: 1 # Reported_hitcount: 1254 #---------------------------------------