Restriction Fragments for FIN1/YDR130C



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The currently selected gene/sequence is :

FIN1/YDR130C on chromosome IV from coordinates 716622 to 715747.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15275.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 15 + MwoI GCNNNNNNNGC 11 8 . . 18 21 + AciI CCGC 18 20 . . 26 31 + Hpy166II GTNNAC 28 28 . . 29 34 + BsaAI YACGTR 31 31 . . 30 33 + TaiI ACGT 33 29 . . 30 33 + SetI ASST 33 29 . . 30 33 + MaeII ACGT 30 32 . . 32 36 + Tsp45I GTSAC 31 36 . . 32 36 + MaeIII GTNAC 31 36 . . 23 33 - BsaXI ACNNNNNCTCC 45 42 15 12 23 34 - TstI CACNNNNNNTCC 47 42 15 10 54 57 + TaqI TCGA 54 56 . . 71 75 + Tsp45I GTSAC 70 75 . . 71 75 + MaeIII GTNAC 70 75 . . 79 87 + Tth111I GACNNNGTC 82 83 . . 107 117 + BsiYI* CCNNNNNNNGG 113 110 . . 105 109 - BslFI GGGAC 90 94 . . 114 117 - MnlI CCTC 107 106 . . 124 127 + CviJI RGCY 125 125 . . 147 150 + SetI ASST 150 146 . . 153 156 - MnlI CCTC 146 145 . . 156 161 + EcoRV GATATC 158 158 . . 162 165 + SetI ASST 165 161 . . 160 164 - HphI GGTGA 152 151 . . 175 179 - BccI CCATC 169 170 . . 181 185 + TspDTI ATGAA 196 194 . . 191 196 + Hpy166II GTNNAC 193 193 . . 191 196 + BssNAI GTATAC 193 193 . . 191 196 + AccI GTMKAC 192 194 . . 199 203 + MaeIII GTNAC 198 203 . . 209 213 - Hin4II* CCTTC 203 202 . . 223 226 + HpaII CCGG 223 225 . . 231 235 + Hpy188I TCNGA 233 232 . . 232 237 + Eco57MI CTGRAG 253 251 . . 232 237 + Eco57I CTGAAG 253 251 . . 234 238 - Hin4II* CCTTC 228 227 . . 238 241 + CviJI RGCY 239 239 . . 244 248 + BsmAI GTCTC 249 253 . . 248 252 + DdeI CTNAG 248 251 . . 262 265 + CviJI RGCY 263 263 . . 264 268 + BssKI CCNGG 263 268 . . 264 268 + BseBI CCWGG 265 266 . . 264 268 + ScrFI CCNGG 265 266 . . 264 268 + EcoRII CCWGG 263 268 . . 271 276 + StuI AGGCCT 273 273 . . 272 275 + CviJI RGCY 273 273 . . 272 275 + HaeIII GGCC 273 273 . . 274 277 + MnlI CCTC 284 283 . . 275 279 + DdeI CTNAG 275 278 . . 275 279 + BspCNI CTCAG 288 286 . . 275 279 + BseMII CTCAG 289 287 . . 285 290 + ApoI RAATTY 285 289 . . 286 289 + TspEI AATT 285 289 . . 288 293 + AsuII TTCGAA 289 291 . . 289 292 + TaqI TCGA 289 291 . . 303 307 + MboII GAAGA 315 314 . . 305 310 + XhoII RGATCY 305 309 . . 306 309 + DpnI GATC 307 307 . . 306 309 + BstKTI GATC 308 306 . . 306 309 + MboI GATC 305 309 . . 306 310 - BinI* GGATC 300 301 . . 315 319 + DdeI CTNAG 315 318 . . 315 319 + BspCNI CTCAG 328 326 . . 315 319 + BseMII CTCAG 329 327 . . 316 320 + Hpy188I TCNGA 318 317 . . 319 322 + DpnI GATC 320 320 . . 319 322 + BstKTI GATC 321 319 . . 319 322 + MboI GATC 318 322 . . 336 341 + SspI AATATT 338 338 . . 349 353 - HphI GGTGA 341 340 . . 356 369 - AjuI GAANNNNNNNTTGG 381 376 349 344 374 378 + TspDTI ATGAA 389 387 . . 391 395 + HphI GGTGA 403 402 . . 394 398 - BccI CCATC 388 389 . . 402 407 + Hpy166II GTNNAC 404 404 . . 402 407 + HpaI GTTAAC 404 404 . . 402 407 + HindII GTYRAC 404 404 . . 403 406 + MseI TTAA 403 405 . . 416 421 + SduI GDGCHC 420 416 . . 416 421 + Bsp120I GGGCCC 416 420 . . 416 421 + NlaIV GGNNCC 418 418 . . 416 420 + BmgT120I GGNCC 417 418 . . 416 421 + HgiJII* GRGCYC 420 416 . . 416 421 + BseSI GKGCMC 420 416 . . 416 420 + AsuI* GGNCC 416 419 . . 416 421 + ApaI GGGCCC 420 416 . . 417 420 + CviJI RGCY 418 418 . . 417 420 + HaeIII GGCC 418 418 . . 417 421 + BmgT120I GGNCC 418 419 . . 417 421 + AsuI* GGNCC 417 420 . . 419 423 + BssKI CCNGG 418 423 . . 419 423 + ScrFI CCNGG 420 421 . . 419 423 + CauII* CCSGG 420 421 . . 420 423 + HpaII CCGG 420 422 . . 425 428 + MseI TTAA 425 427 . . 434 438 + HinfI GANTC 434 437 . . 434 443 + PshAI GACNNNNGTC 438 438 . . 434 438 - PleI GAGTC 428 429 . . 434 438 - MlyI GAGTC 428 428 . . 438 441 + FatI CATG 437 441 . . 438 441 + NlaIII CATG 441 437 . . 438 441 + CviAII CATG 438 440 . . 441 445 + Tsp45I GTSAC 440 445 . . 441 445 + MaeIII GTNAC 440 445 . . 444 447 + SetI ASST 447 443 . . 442 446 - HphI GGTGA 434 433 . . 457 462 + EcoP15I CAGCAG 487 489 . . 452 457 - BciVI GTATCC 446 445 . . 459 469 + MwoI GCNNNNNNNGC 465 462 . . 459 463 + BlsI GCNGC 461 460 . . 459 463 + BisI GCNGC 460 461 . . 459 463 + BbvI GCAGC 471 475 . . 459 463 + TseI GCWGC 459 462 . . 462 472 + MwoI GCNNNNNNNGC 468 465 . . 466 471 + EcoP15I CAGCAG 496 498 . . 468 472 + BlsI GCNGC 470 469 . . 468 472 + BisI GCNGC 469 470 . . 468 472 + BbvI GCAGC 480 484 . . 468 472 + TseI GCWGC 468 471 . . 474 484 + BsaXI ACNNNNNCTCC 464 461 494 491 484 488 + BccI CCATC 492 493 . . 487 491 + Hpy188I TCNGA 489 488 . . 489 493 + FokI GGATG 502 506 . . 489 493 + BseGI GGATG 495 493 . . 490 494 - SfaNI GCATC 480 484 . . 504 510 + TspRI CASTGNN 510 501 . . 509 512 + SetI ASST 512 508 . . 510 513 + MnlI CCTC 520 519 . . 524 528 + DdeI CTNAG 524 527 . . 526 531 + SacI GAGCTC 530 526 . . 526 531 + Ecl136II GAGCTC 528 528 . . 526 531 + SduI GDGCHC 530 526 . . 526 531 + HgiJII* GRGCYC 530 526 . . 526 531 + HgiAI* GWGCWC 530 526 . . 527 530 + SetI ASST 530 526 . . 527 530 + CviJI RGCY 528 528 . . 527 530 + AluI AGCT 528 528 . . 524 528 - BspCNI CTCAG 516 514 . . 524 528 - BseMII CTCAG 515 513 . . 529 534 + SmlI CTYRAG 529 533 . . 529 534 - Bce83I* CTTGAG 514 512 . . 535 539 + HgaI GACGC 544 549 . . 535 540 + AcyI GRCGYC 536 538 . . 554 557 + SetI ASST 557 553 . . 555 559 + Hin4II* CCTTC 565 564 . . 559 566 + PspXI VCTCGAGB 560 564 . . 559 562 + MnlI CCTC 569 568 . . 560 565 + SmlI CTYRAG 560 564 . . 560 565 + XhoI CTCGAG 560 564 . . 560 565 + BmeT110I CYCGRG 561 563 . . 560 565 + AvaI CYCGRG 560 564 . . 561 564 + TaqI TCGA 561 563 . . 564 567 + SetI ASST 567 563 . . 564 567 + CviJI RGCY 565 565 . . 564 567 + AluI AGCT 565 565 . . 569 572 + TspEI AATT 568 572 . . 579 583 + MboII GAAGA 591 590 . . 582 587 + ApoI RAATTY 582 586 . . 583 586 + TspEI AATT 582 586 . . 598 603 + SmlI CTYRAG 598 602 . . 599 604 + Hpy178III* TCNNGA 600 602 . . 598 603 - Bce83I* CTTGAG 583 581 . . 607 611 + MboII GAAGA 619 618 . . 607 612 - Ksp632I* CTCTTC 602 605 . . 610 613 - MnlI CCTC 603 602 . . 613 617 + MboII GAAGA 625 624 . . 613 618 + BbvII* GAAGAC 620 624 . . 617 620 + TaiI ACGT 620 616 . . 617 620 + SetI ASST 620 616 . . 617 620 + MaeII ACGT 617 619 . . 616 621 - BtrI CACGTC 618 618 . . 634 638 + TfiI GAWTC 634 637 . . 634 638 + HinfI GANTC 634 637 . . 649 652 + TaqI TCGA 649 651 . . 663 668 + EcoRI GAATTC 663 667 . . 663 668 + ApoI RAATTY 663 667 . . 664 667 + TspEI AATT 663 667 . . 669 672 + FatI CATG 668 672 . . 669 672 + NlaIII CATG 672 668 . . 669 672 + CviAII CATG 669 671 . . 672 677 + Cac8I GCNNGC 674 674 . . 682 694 + BarI GAAGNNNNNNTAC 674 669 706 701 684 687 + SetI ASST 687 683 . . 682 686 - Hin4II* CCTTC 676 675 . . 699 702 + SetI ASST 702 698 . . 699 702 + CviJI RGCY 700 700 . . 699 702 + AluI AGCT 700 700 . . 701 704 + MaeI CTAG 701 703 . . 707 712 + Hpy178III* TCNNGA 708 710 . . 721 726 + ApoI RAATTY 721 725 . . 722 725 + TspEI AATT 721 725 . . 724 729 + AhaIII* TTTAAA 726 726 . . 725 728 + MseI TTAA 725 727 . . 732 735 + SetI ASST 735 731 . . 745 749 + Tsp45I GTSAC 744 749 . . 745 749 + MaeIII GTNAC 744 749 . . 749 754 + Eco57MI CTGRAG 770 768 . . 749 754 + Eco57I CTGAAG 770 768 . . 751 755 + MboII GAAGA 763 762 . . 757 761 + Tsp4CI* ACNGT 759 758 . . 758 763 + DsaI* CCRYGG 758 762 . . 758 763 + SecI* CCNNGG 758 762 . . 767 770 + MseI TTAA 767 769 . . 803 807 + MboII GAAGA 815 814 . . 803 808 + BbvII* GAAGAC 810 814 . . 820 824 + TfiI GAWTC 820 823 . . 820 824 + HinfI GANTC 820 823 . . 823 828 + Hpy178III* TCNNGA 824 826 . . 814 824 - Hin4I GAYNNNNNVTC 837 832 805 800 839 842 + TaqI TCGA 839 841 . . 841 852 + PpiI GAACNNNNNCTC 833 828 865 860 853 857 + TspGWI ACGGA 868 866 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 192 684 # # AciI: # [CCGC] # 18 858 # # AcyI: # [GRCGYC] # 340 536 # # AhaIII*: # [TTTAAA] # 150 726 # # AjuI: # [GAANNNNNNNTTGG] # 32 349 495 # # AluI: # [AGCT] # 37 135 176 528 # # ApaI: # [GGGCCC] # 420 456 # # ApoI: # [RAATTY] # 58 81 155 285 297 # # AsuI*: # [GGNCC] # 1 416 459 # # AsuII: # [TTCGAA] # 289 587 # # AvaI: # [CYCGRG] # 316 560 # # BarI: # [GAAGNNNNNNTAC] # 32 170 674 # # BbvI: # [GCAGC] # 9 396 471 # # BbvII*: # [GAAGAC] # 66 190 620 # # BccI: # [CCATC] # 104 169 219 384 # # Bce83I*: # [CTTGAG] # 69 293 514 # # BciVI: # [GTATCC] # 430 446 # # BinI*: # [GGATC] # 300 576 # # BisI: # [GCNGC] # 9 407 460 # # BlsI: # [GCNGC] # 9 406 461 # # BmeT110I: # [CYCGRG] # 315 561 # # BmgT120I: # [GGNCC] # 1 417 458 # # BsaAI: # [YACGTR] # 31 845 # # BsaXI: # [ACNNNNNCTCC] # 15 30 30 382 419 # # BseBI: # [CCWGG] # 265 611 # # BseGI: # [GGATG] # 381 495 # # BseMII: # [CTCAG] # 40 186 289 361 # # BseSI: # [GKGCMC] # 420 456 # # BsiYI*: # [CCNNNNNNNGG] # 113 763 # # BslFI: # [GGGAC] # 90 786 # # BsmAI: # [GTCTC] # 249 627 # # Bsp120I: # [GGGCCC] # 416 460 # # BspCNI: # [CTCAG] # 40 188 288 360 # # BssKI: # [CCNGG] # 155 263 458 # # BssNAI: # [GTATAC] # 193 683 # # BstKTI: # [GATC] # 13 308 555 # # BtrI: # [CACGTC] # 258 618 # # Cac8I: # [GCNNGC] # 202 674 # # CauII*: # [CCSGG] # 420 456 # # CviAII: # [CATG] # 207 231 438 # # CviJI: # [RGCY] # 10 24 37 110 114 125 # 135 145 176 # # DdeI: # [CTNAG] # 27 40 209 248 352 # # DpnI: # [GATC] # 13 307 556 # # DsaI*: # [CCRYGG] # 118 758 # # Ecl136II: # [GAGCTC] # 348 528 # # Eco57I: # [CTGAAG] # 106 253 517 # # Eco57MI: # [CTGRAG] # 106 253 517 # # EcoP15I: # [CAGCAG] # 9 380 487 # # EcoRI: # [GAATTC] # 213 663 # # EcoRII: # [CCWGG] # 263 613 # # EcoRV: # [GATATC] # 158 718 # # FatI: # [CATG] # 208 231 437 # # FokI: # [GGATG] # 374 502 # # HaeIII: # [GGCC] # 145 273 458 # # HgaI: # [GACGC] # 332 544 # # HgiAI*: # [GWGCWC] # 346 530 # # HgiJII*: # [GRGCYC] # 110 346 420 # # Hin4I: # [GAYNNNNNVTC] # 32 39 805 # # Hin4II*: # [CCTTC] # 25 111 200 203 337 # # HindII: # [GTYRAC] # 404 472 # # HinfI: # [GANTC] # 56 186 200 434 # # HpaI: # [GTTAAC] # 404 472 # # HpaII: # [CCGG] # 197 223 456 # # HphI: # [GGTGA] # 31 62 152 189 442 # # Hpy166II: # [GTNNAC] # 28 165 211 472 # # Hpy178III*: # [TCNNGA] # 52 108 116 600 # # Hpy188I: # [TCNGA] # 85 171 233 387 # # Ksp632I*: # [CTCTTC] # 274 602 # # MaeI: # [CTAG] # 175 701 # # MaeII: # [ACGT] # 30 259 587 # # MaeIII: # [GTNAC] # 31 39 128 132 242 304 # # MboI: # [GATC] # 13 305 558 # # MboII: # [GAAGA] # 6 28 52 61 138 276 # 315 # # MlyI: # [GAGTC] # 428 448 # # MnlI: # [CCTC] # 34 39 49 107 138 236 # 273 # # MseI: # [TTAA] # 22 42 109 300 403 # # MwoI: # [GCNNNNNNNGC] # 3 11 408 454 # # NlaIII: # [CATG] # 204 231 441 # # NlaIV: # [GGNNCC] # 418 458 # # PleI: # [GAGTC] # 428 448 # # PpiI: # [GAACNNNNNCTC] # 11 32 833 # # PshAI: # [GACNNNNGTC] # 438 438 # # PspXI: # [VCTCGAGB] # 316 560 # # SacI: # [GAGCTC] # 346 530 # # ScrFI: # [CCNGG] # 155 265 456 # # SduI: # [GDGCHC] # 110 346 420 # # SecI*: # [CCNNGG] # 118 758 # # SetI: # [ASST] # 10 15 15 18 27 33 # 33 53 65 67 117 141 # 282 # # SfaNI: # [GCATC] # 396 480 # # SmlI: # [CTYRAG] # 31 38 278 529 # # SspI: # [AATATT] # 338 538 # # StuI: # [AGGCCT] # 273 603 # # TaiI: # [ACGT] # 33 256 587 # # TaqI: # [TCGA] # 37 54 88 190 235 272 # # TfiI: # [GAWTC] # 56 186 634 # # TseI: # [GCWGC] # 9 408 459 # # Tsp45I: # [GTSAC] # 31 39 132 304 370 # # Tsp4CI*: # [ACNGT] # 117 759 # # TspDTI: # [ATGAA] # 193 196 487 # # TspEI: # [AATT] # 14 58 81 155 283 285 # # TspGWI: # [ACGGA] # 8 868 # # TspRI: # [CASTGNN] # 366 510 # # TstI: # [CACNNNNNNTCC] # 15 32 829 # # Tth111I: # [GACNNNGTC] # 82 794 # # XhoI: # [CTCGAG] # 316 560 # # XhoII: # [RGATCY] # 305 571 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 876 # Reported_sequences: 1 # Reported_hitcount: 212 #---------------------------------------