Restriction Fragments for STE5/YDR103W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

STE5/YDR103W on chromosome IV from coordinates 658350 to 661103.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.6562.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 14 19 + SfeI* CTRYAG 14 18 . . 43 46 + TspEI AATT 42 46 . . 52 55 + SetI ASST 55 51 . . 52 55 + CviJI RGCY 53 53 . . 52 55 + AluI AGCT 53 53 . . 55 58 + TaqI TCGA 55 57 . . 70 75 + KpnI GGTACC 74 70 . . 70 75 + NlaIV GGNNCC 72 72 . . 70 75 + HgiCI* GGYRCC 70 74 . . 70 75 + Acc65I GGTACC 70 74 . . 71 74 + Csp6I GTAC 71 73 . . 71 74 + RsaI GTAC 72 72 . . 73 76 + SetI ASST 76 72 . . 79 82 + TaqI TCGA 79 81 . . 79 84 + Hpy178III* TCNNGA 80 82 . . 104 107 + SetI ASST 107 103 . . 104 107 + CviJI RGCY 105 105 . . 104 107 + AluI AGCT 105 105 . . 105 115 + MwoI GCNNNNNNNGC 111 108 . . 106 109 + MaeI CTAG 106 108 . . 113 116 + CviJI RGCY 114 114 . . 118 123 + TatI WGTACW 118 122 . . 118 123 + ScaI AGTACT 120 120 . . 119 122 + Csp6I GTAC 119 121 . . 119 122 + RsaI GTAC 120 120 . . 116 120 - BsrI ACTGG 116 114 . . 115 120 - BfiI ACTGGG 110 109 . . 136 141 + Hpy178III* TCNNGA 137 139 . . 140 143 - MnlI CCTC 133 132 . . 154 158 + TspGWI ACGGA 169 167 . . 164 169 + TsoI TARCCA 180 178 . . 165 168 + CviJI RGCY 166 166 . . 167 171 + BssKI CCNGG 166 171 . . 167 171 + BseBI CCWGG 168 169 . . 167 171 + ScrFI CCNGG 168 169 . . 167 171 + EcoRII CCWGG 166 171 . . 169 172 + SetI ASST 172 168 . . 170 175 + NlaIV GGNNCC 172 172 . . 195 204 + XmnI GAANNNNTTC 199 199 . . 200 204 + TfiI GAWTC 200 203 . . 200 204 + HinfI GANTC 200 203 . . 207 210 + SetI ASST 210 206 . . 208 212 + Hin4II* CCTTC 218 217 . . 205 209 - HphI GGTGA 197 196 . . 221 224 + MnlI CCTC 231 230 . . 224 227 + MnlI CCTC 234 233 . . 222 227 - BseRI GAGGAG 213 211 . . 242 247 + TaqII CACCCA 258 256 . . 241 245 - HphI GGTGA 233 232 . . 245 255 + BsiYI* CCNNNNNNNGG 251 248 . . 251 254 + MaeI CTAG 251 253 . . 256 260 + Tsp45I GTSAC 255 260 . . 256 260 + MaeIII GTNAC 255 260 . . 262 266 - MboII GAAGA 254 253 . . 270 275 + Ksp632I* CTCTTC 276 279 . . 271 275 - MboII GAAGA 263 262 . . 278 283 + BsrDI GCAATG 285 283 . . 281 285 + TspDTI ATGAA 296 294 . . 283 287 + MboII GAAGA 295 294 . . 283 288 + BbvII* GAAGAC 290 294 . . 287 291 + Tsp4CI* ACNGT 289 288 . . 289 295 + BstEII GGTNACC 289 294 . . 290 294 + MaeIII GTNAC 289 294 . . 293 296 + SetI ASST 296 292 . . 298 302 + TspDTI ATGAA 313 311 . . 306 309 + SetI ASST 309 305 . . 307 311 + Hin4II* CCTTC 317 316 . . 313 317 + Tsp4CI* ACNGT 315 314 . . 321 327 + TspRI CASTGNN 327 318 . . 343 346 + MnlI CCTC 353 352 . . 369 372 + SetI ASST 372 368 . . 373 376 + TspEI AATT 372 376 . . 364 377 - AjuI GAANNNNNNNTTGG 389 384 357 352 401 405 + MaeIII GTNAC 400 405 . . 404 407 + SetI ASST 407 403 . . 409 414 + Hpy178III* TCNNGA 410 412 . . 410 415 + BmeT110I CYCGRG 411 413 . . 410 415 + AvaI CYCGRG 410 414 . . 421 426 + SduI GDGCHC 425 421 . . 421 426 + HgiJII* GRGCYC 425 421 . . 422 425 + CviJI RGCY 423 423 . . 427 432 + StyI CCWWGG 427 431 . . 427 432 + SecI* CCNNGG 427 431 . . 431 435 + BmgT120I GGNCC 432 433 . . 431 435 + AsuI* GGNCC 431 434 . . 432 435 + CviJI RGCY 433 433 . . 432 435 + HaeIII GGCC 433 433 . . 438 443 + ApoI RAATTY 438 442 . . 439 442 + TspEI AATT 438 442 . . 445 449 + Hpy188I TCNGA 447 446 . . 458 463 + TaqII CACCCA 474 472 . . 461 466 + StyI CCWWGG 461 465 . . 461 466 + SecI* CCNNGG 461 465 . . 464 467 + SetI ASST 467 463 . . 478 481 + CviJI RGCY 479 479 . . 477 482 - TsoI TARCCA 467 465 . . 496 499 + SetI ASST 499 495 . . 497 500 + MnlI CCTC 507 506 . . 503 506 + MseI TTAA 503 505 . . 519 524 + BsmI GAATGC 525 523 . . 530 533 + Csp6I GTAC 530 532 . . 530 533 + RsaI GTAC 531 531 . . 532 535 + TaiI ACGT 535 531 . . 532 535 + SetI ASST 535 531 . . 532 535 + MaeII ACGT 532 534 . . 539 543 + Tsp45I GTSAC 538 543 . . 539 543 + MaeIII GTNAC 538 543 . . 545 548 + CviJI RGCY 546 546 . . 560 564 + MboII GAAGA 572 571 . . 576 579 + TspEI AATT 575 579 . . 584 587 + SetI ASST 587 583 . . 584 587 + CviJI RGCY 585 585 . . 584 587 + AluI AGCT 585 585 . . 585 590 + Cac8I GCNNGC 587 587 . . 588 591 + CviRI* TGCA 589 589 . . 589 594 + NspI RCATGY 593 589 . . 590 593 + FatI CATG 589 593 . . 590 593 + NlaIII CATG 593 589 . . 590 593 + CviAII CATG 590 592 . . 594 599 + BalI TGGCCA 596 596 . . 594 599 + CfrI YGGCCR 594 598 . . 595 598 + CviJI RGCY 596 596 . . 595 598 + HaeIII GGCC 596 596 . . 600 605 + SmlI CTYRAG 600 604 . . 600 605 + AflII CTTAAG 600 604 . . 601 604 + MseI TTAA 601 603 . . 605 609 + Tsp45I GTSAC 604 609 . . 605 609 + MaeIII GTNAC 604 609 . . 606 610 - HphI GGTGA 598 597 . . 611 621 + FalI AAGNNNNNCTT 602 597 634 629 611 621 - FalI AAGNNNNNCTT 634 629 602 597 634 639 + NlaIV GGNNCC 636 636 . . 634 639 + HgiCI* GGYRCC 634 638 . . 653 656 + TaiI ACGT 656 652 . . 653 656 + SetI ASST 656 652 . . 653 656 + MaeII ACGT 653 655 . . 661 664 + Hin6I GCGC 661 663 . . 661 664 + GlaI GCGC 662 662 . . 661 664 + HhaI GCGC 663 661 . . 677 680 + Csp6I GTAC 677 679 . . 677 680 + RsaI GTAC 678 678 . . 705 708 + CviJI RGCY 706 706 . . 706 710 - BceAI ACGGC 691 693 . . 714 719 + EcoT22I ATGCAT 718 714 . . 715 718 + CviRI* TGCA 716 716 . . 720 725 + Hpy178III* TCNNGA 721 723 . . 716 721 - BsmI GAATGC 716 714 . . 731 735 + TspDTI ATGAA 746 744 . . 750 753 + TspEI AATT 749 753 . . 754 758 + Hpy188I TCNGA 756 755 . . 765 769 + TfiI GAWTC 765 768 . . 765 769 + HinfI GANTC 765 768 . . 767 771 - TspDTI ATGAA 757 755 . . 774 778 + TfiI GAWTC 774 777 . . 774 778 + HinfI GANTC 774 777 . . 777 782 + Hpy178III* TCNNGA 778 780 . . 781 785 + TfiI GAWTC 781 784 . . 781 785 + HinfI GANTC 781 784 . . 784 788 + Hpy188I TCNGA 786 785 . . 785 789 + DdeI CTNAG 785 788 . . 785 789 - BspCNI CTCAG 777 775 . . 785 789 - BseMII CTCAG 776 774 . . 801 804 + SetI ASST 804 800 . . 802 805 + MnlI CCTC 812 811 . . 803 807 + DdeI CTNAG 803 806 . . 803 807 + BspCNI CTCAG 816 814 . . 803 807 + BseMII CTCAG 817 815 . . 809 813 + FauI CCCGC 817 819 . . 810 813 + AciI CCGC 810 812 . . 807 811 - BslFI GGGAC 792 796 . . 817 820 + MnlI CCTC 827 826 . . 826 830 - HphI GGTGA 818 817 . . 838 841 + MnlI CCTC 848 847 . . 838 848 + BsiYI* CCNNNNNNNGG 844 841 . . 861 864 + SetI ASST 864 860 . . 879 889 + Hin4I GAYNNNNNVTC 870 865 902 897 887 892 + SmlI CTYRAG 887 891 . . 891 894 + SetI ASST 894 890 . . 891 894 + CviJI RGCY 892 892 . . 891 894 + AluI AGCT 892 892 . . 887 892 - Bce83I* CTTGAG 872 870 . . 921 924 + CviJI RGCY 922 922 . . 922 926 + BlsI GCNGC 924 923 . . 922 926 + BisI GCNGC 923 924 . . 922 926 + TseI GCWGC 922 925 . . 924 927 + CviRI* TGCA 925 925 . . 922 926 - BbvI GCAGC 909 913 . . 923 928 - BsgI GTGCAG 908 906 . . 932 937 + StyI CCWWGG 932 936 . . 932 937 + SecI* CCNNGG 932 936 . . 947 952 + ApoI RAATTY 947 951 . . 948 951 + TspEI AATT 947 951 . . 989 995 + PasI CCCWGGG 990 993 . . 989 994 + SecI* CCNNGG 989 993 . . 985 989 - HphI GGTGA 977 976 . . 990 994 + BssKI CCNGG 989 994 . . 990 994 + BseBI CCWGG 991 992 . . 990 995 + SecI* CCNNGG 990 994 . . 990 994 + ScrFI CCNGG 991 992 . . 990 994 + EcoRII CCWGG 989 994 . . 994 999 + SduI GDGCHC 998 994 . . 994 999 + BseSI GKGCMC 998 994 . . 1001 1005 + MboII GAAGA 1013 1012 . . 1004 1009 + EcoRI GAATTC 1004 1008 . . 1004 1009 + ApoI RAATTY 1004 1008 . . 1005 1008 + TspEI AATT 1004 1008 . . 1008 1012 - TspGWI ACGGA 998 996 . . 1012 1017 + Hpy178III* TCNNGA 1013 1015 . . 1012 1017 + BetI* WCCGGW 1012 1016 . . 1012 1017 + BspMII* TCCGGA 1012 1016 . . 1013 1016 + HpaII CCGG 1013 1015 . . 1029 1033 + DdeI CTNAG 1029 1032 . . 1039 1042 + SetI ASST 1042 1038 . . 1040 1043 + MnlI CCTC 1050 1049 . . 1043 1048 + SfeI* CTRYAG 1043 1047 . . 1048 1058 + MwoI GCNNNNNNNGC 1054 1051 . . 1056 1059 + SetI ASST 1059 1055 . . 1056 1059 + CviJI RGCY 1057 1057 . . 1056 1059 + AluI AGCT 1057 1057 . . 1063 1067 + TfiI GAWTC 1063 1066 . . 1063 1067 + HinfI GANTC 1063 1066 . . 1073 1078 - EcoP15I CAGCAG 1045 1047 . . 1084 1087 + TspEI AATT 1083 1087 . . 1101 1105 + FokI GGATG 1114 1118 . . 1101 1105 + BseGI GGATG 1107 1105 . . 1119 1124 + AclI AACGTT 1120 1122 . . 1120 1123 + TaiI ACGT 1123 1119 . . 1120 1123 + SetI ASST 1123 1119 . . 1120 1123 + MaeII ACGT 1120 1122 . . 1125 1129 + TauI GCSGC 1128 1125 . . 1125 1129 + BlsI GCNGC 1127 1126 . . 1125 1129 + BisI GCNGC 1126 1127 . . 1126 1131 + NspBII* CMGCKG 1128 1128 . . 1126 1129 + AciI CCGC 1126 1128 . . 1132 1135 + MseI TTAA 1132 1134 . . 1135 1138 + SetI ASST 1138 1134 . . 1151 1156 + ApoI RAATTY 1151 1155 . . 1152 1155 + TspEI AATT 1151 1155 . . 1161 1170 + XmnI GAANNNNTTC 1165 1165 . . 1165 1168 + TspEI AATT 1164 1168 . . 1169 1175 + PfoI TCCNGGA 1169 1174 . . 1170 1174 + BssKI CCNGG 1169 1174 . . 1170 1174 + BseBI CCWGG 1171 1172 . . 1170 1174 + ScrFI CCNGG 1171 1172 . . 1170 1174 + EcoRII CCWGG 1169 1174 . . 1174 1178 + MboII GAAGA 1186 1185 . . 1178 1181 + TspEI AATT 1177 1181 . . 1181 1184 + CviRI* TGCA 1182 1182 . . 1202 1206 + BslFI GGGAC 1216 1220 . . 1216 1220 + DdeI CTNAG 1216 1219 . . 1219 1222 + SetI ASST 1222 1218 . . 1225 1230 + Hpy166II GTNNAC 1227 1227 . . 1225 1230 + AccI GTMKAC 1226 1228 . . 1232 1235 + TspEI AATT 1231 1235 . . 1249 1253 - BccI CCATC 1243 1244 . . 1254 1258 + Hpy188I TCNGA 1256 1255 . . 1288 1292 + MboII GAAGA 1300 1299 . . 1288 1293 + BbvII* GAAGAC 1295 1299 . . 1291 1295 + HgaI GACGC 1300 1305 . . 1338 1341 + TspEI AATT 1337 1341 . . 1350 1355 + ApoI RAATTY 1350 1354 . . 1351 1354 + TspEI AATT 1350 1354 . . 1368 1371 + SetI ASST 1371 1367 . . 1380 1385 + SmlI CTYRAG 1380 1384 . . 1380 1385 + Bce83I* CTTGAG 1401 1399 . . 1397 1400 + TaqI TCGA 1397 1399 . . 1399 1411 + BarI GAAGNNNNNNTAC 1391 1386 1423 1418 1400 1405 + HindIII AAGCTT 1400 1404 . . 1401 1404 + SetI ASST 1404 1400 . . 1401 1404 + CviJI RGCY 1402 1402 . . 1401 1404 + AluI AGCT 1402 1402 . . 1407 1412 + TatI WGTACW 1407 1411 . . 1407 1412 + ScaI AGTACT 1409 1409 . . 1403 1408 - Eco57MI CTGRAG 1388 1386 . . 1408 1411 + Csp6I GTAC 1408 1410 . . 1408 1411 + RsaI GTAC 1409 1409 . . 1403 1408 - Eco57I CTGAAG 1388 1386 . . 1417 1420 + CviRI* TGCA 1418 1418 . . 1420 1423 + SetI ASST 1423 1419 . . 1423 1426 + MseI TTAA 1423 1425 . . 1439 1442 + Hin6I GCGC 1439 1441 . . 1439 1442 + GlaI GCGC 1440 1440 . . 1439 1442 + HhaI GCGC 1441 1439 . . 1435 1440 - BsrDI GCAATG 1434 1432 . . 1450 1454 + Hpy188I TCNGA 1452 1451 . . 1452 1457 + XhoII RGATCY 1452 1456 . . 1452 1457 + BglII AGATCT 1452 1456 . . 1453 1456 + DpnI GATC 1454 1454 . . 1453 1456 + BstKTI GATC 1455 1453 . . 1453 1456 + MboI GATC 1452 1456 . . 1467 1471 + Hpy188I TCNGA 1469 1468 . . 1476 1481 + ApoI RAATTY 1476 1480 . . 1477 1480 + TspEI AATT 1476 1480 . . 1480 1484 + Hpy188I TCNGA 1482 1481 . . 1495 1499 + Tsp4CI* ACNGT 1497 1496 . . 1497 1502 + TatI WGTACW 1497 1501 . . 1497 1502 + Bsp1407I TGTACA 1497 1501 . . 1498 1501 + Csp6I GTAC 1498 1500 . . 1498 1501 + RsaI GTAC 1499 1499 . . 1509 1514 + EcoRV GATATC 1511 1511 . . 1515 1518 + SetI ASST 1518 1514 . . 1524 1528 + TfiI GAWTC 1524 1527 . . 1524 1528 + HinfI GANTC 1524 1527 . . 1527 1532 + Hpy178III* TCNNGA 1528 1530 . . 1546 1549 - MnlI CCTC 1539 1538 . . 1561 1564 + TaqI TCGA 1561 1563 . . 1575 1579 - MboII GAAGA 1567 1566 . . 1583 1588 + PsiI TTATAA 1585 1585 . . 1621 1626 + BsiI* CACGAG 1621 1625 . . 1624 1628 - BsmAI GTCTC 1618 1622 . . 1624 1629 - Esp3I CGTCTC 1618 1622 . . 1631 1634 + Csp6I GTAC 1631 1633 . . 1631 1634 + RsaI GTAC 1632 1632 . . 1633 1636 + SetI ASST 1636 1632 . . 1639 1642 + MaeI CTAG 1639 1641 . . 1641 1644 + SetI ASST 1644 1640 . . 1645 1648 + MseI TTAA 1645 1647 . . 1672 1677 - MmeI TCCRAC 1653 1651 . . 1681 1686 + Hpy166II GTNNAC 1683 1683 . . 1681 1686 + SduI GDGCHC 1685 1681 . . 1681 1686 + BseSI GKGCMC 1685 1681 . . 1681 1686 + ApaLI GTGCAC 1681 1685 . . 1681 1686 + HgiAI* GWGCWC 1685 1681 . . 1682 1685 + CviRI* TGCA 1683 1683 . . 1684 1693 + MslI CAYNNNNRTG 1688 1688 . . 1696 1700 + Tsp4CI* ACNGT 1698 1697 . . 1710 1713 - MnlI CCTC 1703 1702 . . 1715 1719 + TfiI GAWTC 1715 1718 . . 1715 1719 + HinfI GANTC 1715 1718 . . 1720 1723 + SetI ASST 1723 1719 . . 1718 1722 - HphI GGTGA 1710 1709 . . 1723 1726 + MseI TTAA 1723 1725 . . 1725 1730 + ApoI RAATTY 1725 1729 . . 1726 1729 + TspEI AATT 1725 1729 . . 1729 1734 + Hpy178III* TCNNGA 1730 1732 . . 1742 1745 + MaeI CTAG 1742 1744 . . 1750 1755 + TatI WGTACW 1750 1754 . . 1750 1755 + ScaI AGTACT 1752 1752 . . 1751 1754 + Csp6I GTAC 1751 1753 . . 1751 1754 + RsaI GTAC 1752 1752 . . 1753 1757 + Tsp4CI* ACNGT 1755 1754 . . 1756 1761 + SalI GTCGAC 1756 1760 . . 1756 1761 + Hpy166II GTNNAC 1758 1758 . . 1756 1761 + HindII GTYRAC 1758 1758 . . 1756 1761 + AccI GTMKAC 1757 1759 . . 1757 1760 + TaqI TCGA 1757 1759 . . 1760 1764 + Tsp4CI* ACNGT 1762 1761 . . 1763 1768 + Hpy166II GTNNAC 1765 1765 . . 1763 1768 + BssNAI GTATAC 1765 1765 . . 1763 1768 + AccI GTMKAC 1764 1766 . . 1778 1783 + TsoI TARCCA 1794 1792 . . 1789 1792 + SetI ASST 1792 1788 . . 1789 1792 + CviJI RGCY 1790 1790 . . 1789 1792 + AluI AGCT 1790 1790 . . 1794 1797 + TspEI AATT 1793 1797 . . 1805 1808 + MseI TTAA 1805 1807 . . 1818 1821 + SetI ASST 1821 1817 . . 1825 1828 + TspEI AATT 1824 1828 . . 1845 1848 + DpnI GATC 1846 1846 . . 1845 1848 + BstKTI GATC 1847 1845 . . 1845 1848 + MboI GATC 1844 1848 . . 1846 1851 + ClaI ATCGAT 1847 1849 . . 1847 1850 + TaqI TCGA 1847 1849 . . 1859 1862 + TspEI AATT 1858 1862 . . 1867 1871 + MboII GAAGA 1879 1878 . . 1863 1867 - Hin4II* CCTTC 1857 1856 . . 1870 1874 + MboII GAAGA 1882 1881 . . 1870 1875 + BbvII* GAAGAC 1877 1881 . . 1874 1878 + Tsp4CI* ACNGT 1876 1875 . . 1879 1882 + MseI TTAA 1879 1881 . . 1881 1884 + TspEI AATT 1880 1884 . . 1890 1895 + PsiI TTATAA 1892 1892 . . 1895 1899 + Tsp4CI* ACNGT 1897 1896 . . 1904 1909 + HindIII AAGCTT 1904 1908 . . 1905 1908 + SetI ASST 1908 1904 . . 1905 1908 + CviJI RGCY 1906 1906 . . 1905 1908 + AluI AGCT 1906 1906 . . 1909 1912 + MseI TTAA 1909 1911 . . 1910 1915 + TsoI TARCCA 1926 1924 . . 1916 1919 + MseI TTAA 1916 1918 . . 1918 1923 + ApoI RAATTY 1918 1922 . . 1919 1922 + TspEI AATT 1918 1922 . . 1924 1927 + Hin6I GCGC 1924 1926 . . 1924 1927 + GlaI GCGC 1925 1925 . . 1924 1927 + HhaI GCGC 1926 1924 . . 1925 1928 + FnuDII* CGCG 1926 1926 . . 1931 1934 + CviRI* TGCA 1932 1932 . . 1948 1951 + DpnI GATC 1949 1949 . . 1948 1951 + BstKTI GATC 1950 1948 . . 1948 1951 + MboI GATC 1947 1951 . . 1950 1953 + TaqI TCGA 1950 1952 . . 1957 1960 + TspEI AATT 1956 1960 . . 1965 1970 + Hpy178III* TCNNGA 1966 1968 . . 1969 1979 + Hin4I GAYNNNNNVTC 1960 1955 1992 1987 1975 1979 + BinI* GGATC 1983 1984 . . 1976 1979 + DpnI GATC 1977 1977 . . 1976 1979 + BstKTI GATC 1978 1976 . . 1976 1979 + MboI GATC 1975 1979 . . 1969 1979 - Hin4I GAYNNNNNVTC 1992 1987 1960 1955 1996 2000 + TfiI GAWTC 1996 1999 . . 1996 2000 + HinfI GANTC 1996 1999 . . 2002 2005 + MaeI CTAG 2002 2004 . . 2005 2009 + TfiI GAWTC 2005 2008 . . 2005 2009 + HinfI GANTC 2005 2008 . . 2005 2015 + Hin4I GAYNNNNNVTC 1996 1991 2028 2023 2009 2013 + BccI CCATC 2017 2018 . . 2036 2039 + MnlI CCTC 2046 2045 . . 2038 2041 + TaqI TCGA 2038 2040 . . 2045 2048 + TspEI AATT 2044 2048 . . 2052 2055 + SetI ASST 2055 2051 . . 2050 2054 - HphI GGTGA 2042 2041 . . 2079 2084 + Hpy178III* TCNNGA 2080 2082 . . 2083 2092 + XmnI GAANNNNTTC 2087 2087 . . 2086 2091 + SspI AATATT 2088 2088 . . 2091 2096 + Hpy178III* TCNNGA 2092 2094 . . 2091 2096 + BspHI TCATGA 2091 2095 . . 2092 2095 + FatI CATG 2091 2095 . . 2092 2095 + NlaIII CATG 2095 2091 . . 2092 2095 + CviAII CATG 2092 2094 . . 2093 2097 + TspDTI ATGAA 2108 2106 . . 2090 2094 - TspDTI ATGAA 2080 2078 . . 2157 2162 + Hpy178III* TCNNGA 2158 2160 . . 2168 2172 - SfaNI GCATC 2158 2162 . . 2186 2190 + MboII GAAGA 2198 2197 . . 2189 2193 + MboII GAAGA 2201 2200 . . 2195 2198 + CviJI RGCY 2196 2196 . . 2195 2198 + HaeIII GGCC 2196 2196 . . 2195 2199 + BmgT120I GGNCC 2196 2197 . . 2195 2199 + AsuI* GGNCC 2195 2198 . . 2192 2196 - Hin4II* CCTTC 2186 2185 . . 2201 2205 + TspDTI ATGAA 2216 2214 . . 2204 2207 + TspEI AATT 2203 2207 . . 2208 2212 + MboII GAAGA 2220 2219 . . 2213 2216 + MseI TTAA 2213 2215 . . 2216 2219 + SetI ASST 2219 2215 . . 2221 2224 + CviJI RGCY 2222 2222 . . 2229 2241 + PsrI GAACNNNNNNTAC 2221 2216 2253 2248 2230 2235 + AclI AACGTT 2231 2233 . . 2231 2234 + TaiI ACGT 2234 2230 . . 2231 2234 + SetI ASST 2234 2230 . . 2231 2234 + MaeII ACGT 2231 2233 . . 2233 2238 + Hpy166II GTNNAC 2235 2235 . . 2233 2238 + HindII GTYRAC 2235 2235 . . 2238 2243 + SfeI* CTRYAG 2238 2242 . . 2240 2244 + Tsp4CI* ACNGT 2242 2241 . . 2241 2247 + TspRI CASTGNN 2247 2238 . . 2250 2253 + TspEI AATT 2249 2253 . . 2255 2259 + TspDTI ATGAA 2270 2268 . . 2259 2264 + SpeI ACTAGT 2259 2263 . . 2260 2263 + MaeI CTAG 2260 2262 . . 2264 2267 + TaqI TCGA 2264 2266 . . 2266 2276 + BplI GAGNNNNNCTC 2257 2252 2289 2284 2268 2271 + CviJI RGCY 2269 2269 . . 2268 2271 + HaeIII GGCC 2269 2269 . . 2266 2269 - MnlI CCTC 2259 2258 . . 2269 2274 + Cac8I GCNNGC 2271 2271 . . 2270 2280 + BsiYI* CCNNNNNNNGG 2276 2273 . . 2270 2280 + PflMI CCANNNNNTGG 2276 2273 . . 2271 2279 + AlwNI CAGNNNCTG 2276 2273 . . 2272 2275 + SetI ASST 2275 2271 . . 2272 2275 + CviJI RGCY 2273 2273 . . 2272 2275 + AluI AGCT 2273 2273 . . 2275 2281 + PfoI TCCNGGA 2275 2280 . . 2276 2280 + BssKI CCNGG 2275 2280 . . 2276 2280 + BseBI CCWGG 2277 2278 . . 2266 2276 - BplI GAGNNNNNCTC 2289 2284 2257 2252 2276 2280 + ScrFI CCNGG 2277 2278 . . 2276 2280 + EcoRII CCWGG 2275 2280 . . 2304 2309 + SfeI* CTRYAG 2304 2308 . . 2306 2310 + Tsp4CI* ACNGT 2308 2307 . . 2312 2317 - TaqII GACCGA 2302 2300 . . 2317 2321 + DdeI CTNAG 2317 2320 . . 2327 2331 + TspDTI ATGAA 2342 2340 . . 2332 2335 + FatI CATG 2331 2335 . . 2332 2335 + NlaIII CATG 2335 2331 . . 2332 2335 + CviAII CATG 2332 2334 . . 2347 2351 + MboII GAAGA 2359 2358 . . 2347 2352 - Ksp632I* CTCTTC 2342 2345 . . 2350 2353 - MnlI CCTC 2343 2342 . . 2362 2366 + TfiI GAWTC 2362 2365 . . 2362 2366 + HinfI GANTC 2362 2365 . . 2365 2368 + TaqI TCGA 2365 2367 . . 2366 2371 + McrI* CGRYCG 2369 2367 . . 2367 2372 + TaqII GACCGA 2383 2381 . . 2375 2379 + TspDTI ATGAA 2390 2388 . . 2392 2397 + Hpy178III* TCNNGA 2393 2395 . . 2395 2399 + BinI* GGATC 2403 2404 . . 2396 2399 + DpnI GATC 2397 2397 . . 2396 2399 + BstKTI GATC 2398 2396 . . 2396 2399 + MboI GATC 2395 2399 . . 2399 2405 + TspRI CASTGNN 2405 2396 . . 2400 2404 + Tsp4CI* ACNGT 2402 2401 . . 2404 2408 + Hpy188I TCNGA 2406 2405 . . 2406 2410 + FokI GGATG 2419 2423 . . 2406 2410 + BseGI GGATG 2412 2410 . . 2407 2417 + Hin4I GAYNNNNNVTC 2398 2393 2430 2425 2403 2408 - MmeI TCCRAC 2384 2382 . . 2407 2411 - SfaNI GCATC 2397 2401 . . 2413 2417 + TfiI GAWTC 2413 2416 . . 2413 2417 + HinfI GANTC 2413 2416 . . 2407 2417 - Hin4I GAYNNNNNVTC 2430 2425 2398 2393 2429 2433 - TspDTI ATGAA 2419 2417 . . 2437 2441 + TfiI GAWTC 2437 2440 . . 2437 2441 + HinfI GANTC 2437 2440 . . 2453 2457 + TspDTI ATGAA 2468 2466 . . 2465 2468 + AciI CCGC 2465 2467 . . 2475 2479 + HphI GGTGA 2487 2486 . . 2489 2492 + SetI ASST 2492 2488 . . 2490 2494 + Hin4II* CCTTC 2500 2499 . . 2496 2502 + TspRI CASTGNN 2502 2493 . . 2498 2502 + DdeI CTNAG 2498 2501 . . 2498 2502 - BspCNI CTCAG 2490 2488 . . 2498 2502 - BseMII CTCAG 2489 2487 . . 2500 2503 - MnlI CCTC 2493 2492 . . 2503 2507 + HphI GGTGA 2515 2514 . . 2504 2509 + Hpy166II GTNNAC 2506 2506 . . 2526 2530 + DdeI CTNAG 2526 2529 . . 2533 2537 + Tsp4CI* ACNGT 2535 2534 . . 2542 2546 - MboII GAAGA 2534 2533 . . 2547 2552 + TatI WGTACW 2547 2551 . . 2543 2548 - Eco57MI CTGRAG 2528 2526 . . 2548 2551 + Csp6I GTAC 2548 2550 . . 2548 2551 + RsaI GTAC 2549 2549 . . 2543 2548 - Eco57I CTGAAG 2528 2526 . . 2552 2555 + CviJI RGCY 2553 2553 . . 2553 2558 + Cac8I GCNNGC 2555 2555 . . 2556 2559 + SetI ASST 2559 2555 . . 2556 2559 + CviJI RGCY 2557 2557 . . 2556 2559 + AluI AGCT 2557 2557 . . 2559 2563 + Hpy188I TCNGA 2561 2560 . . 2562 2566 + TfiI GAWTC 2562 2565 . . 2562 2566 + HinfI GANTC 2562 2565 . . 2569 2574 + SspI AATATT 2571 2571 . . 2585 2589 - TspDTI ATGAA 2575 2573 . . 2590 2594 + Hpy188I TCNGA 2592 2591 . . 2591 2595 + DdeI CTNAG 2591 2594 . . 2593 2598 + BseRI GAGGAG 2608 2606 . . 2591 2595 - BspCNI CTCAG 2583 2581 . . 2591 2595 - BseMII CTCAG 2582 2580 . . 2593 2596 - MnlI CCTC 2586 2585 . . 2596 2599 - MnlI CCTC 2589 2588 . . 2601 2604 + SetI ASST 2604 2600 . . 2599 2603 - Hin4II* CCTTC 2593 2592 . . 2603 2606 + Csp6I GTAC 2603 2605 . . 2603 2606 + RsaI GTAC 2604 2604 . . 2609 2613 + TspDTI ATGAA 2624 2622 . . 2615 2619 + TspDTI ATGAA 2630 2628 . . 2621 2625 + TspDTI ATGAA 2636 2634 . . 2635 2639 - BsrI ACTGG 2635 2633 . . 2647 2651 + DdeI CTNAG 2647 2650 . . 2666 2670 + TfiI GAWTC 2666 2669 . . 2666 2670 + HinfI GANTC 2666 2669 . . 2668 2673 + ClaI ATCGAT 2669 2671 . . 2669 2672 + TaqI TCGA 2669 2671 . . 2671 2675 - BccI CCATC 2665 2666 . . 2678 2683 + TsoI TARCCA 2694 2692 . . 2684 2688 + HgaI GACGC 2693 2698 . . 2691 2695 - TspDTI ATGAA 2681 2679 . . 2696 2701 + Hpy178III* TCNNGA 2697 2699 . . 2697 2702 + SmlI CTYRAG 2697 2701 . . 2697 2702 + XhoI CTCGAG 2697 2701 . . 2697 2702 + BmeT110I CYCGRG 2698 2700 . . 2697 2702 + AvaI CYCGRG 2697 2701 . . 2698 2701 + TaqI TCGA 2698 2700 . . 2700 2704 + PleI GAGTC 2708 2709 . . 2700 2704 + MlyI GAGTC 2709 2709 . . 2700 2704 + HinfI GANTC 2700 2703 . . 2714 2717 - AciI CCGC 2714 2716 . . 2712 2717 - BsrBI CCGCTC 2714 2714 . . 2726 2730 + Tsp4CI* ACNGT 2728 2727 . . 2733 2736 + MnlI CCTC 2743 2742 . . 2729 2733 - BslFI GGGAC 2714 2718 . . 2733 2743 + BsiYI* CCNNNNNNNGG 2739 2736 . . 2737 2742 + NdeI CATATG 2738 2740 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 70 2684 # # AccI: # [GTMKAC] # 7 531 990 1226 # # AciI: # [CCGC] # 40 249 316 810 1339 # # AclI: # [AACGTT] # 523 1111 1120 # # AflII: # [CTTAAG] # 600 2154 # # AjuI: # [GAANNNNNNNTTGG] # 32 357 2365 # # AluI: # [AGCT] # 52 53 116 165 197 284 # 307 345 367 388 480 # # AlwNI: # [CAGNNNCTG] # 478 2276 # # ApaLI: # [GTGCAC] # 1073 1681 # # ApoI: # [RAATTY] # 57 126 147 193 199 249 # 438 509 836 # # AsuI*: # [GGNCC] # 431 559 1764 # # AvaI: # [CYCGRG] # 57 410 2287 # # BalI: # [TGGCCA] # 596 2158 # # BarI: # [GAAGNNNNNNTAC] # 32 1331 1391 # # BbvI: # [GCAGC] # 909 1845 # # BbvII*: # [GAAGAC] # 290 582 877 1005 # # BccI: # [CCATC] # 89 648 774 1243 # # Bce83I*: # [CTTGAG] # 529 872 1353 # # BceAI: # [ACGGC] # 691 2063 # # BetI*: # [WCCGGW] # 1012 1742 # # BfiI: # [ACTGGG] # 110 2644 # # BglII: # [AGATCT] # 1302 1452 # # BinI*: # [GGATC] # 351 420 1983 # # BisI: # [GCNGC] # 203 923 1628 # # BlsI: # [GCNGC] # 203 924 1627 # # BmeT110I: # [CYCGRG] # 56 411 2287 # # BmgT120I: # [GGNCC] # 432 558 1764 # # BplI: # [GAGNNNNNCTC] # 0 0 32 465 2257 # # BseBI: # [CCWGG] # 168 180 477 823 1106 # # BseGI: # [GGATG] # 342 1107 1305 # # BseMII: # [CTCAG] # 41 93 172 776 1672 # # BseRI: # [GAGGAG] # 146 213 2395 # # BseSI: # [GKGCMC] # 687 998 1069 # # BsgI: # [GTGCAG] # 908 1846 # # BsiI*: # [CACGAG] # 1133 1621 # # BsiYI*: # [CCNNNNNNNGG] # 15 251 463 593 1432 # # BslFI: # [GGGAC] # 40 424 792 1498 # # BsmAI: # [GTCTC] # 1136 1618 # # BsmI: # [GAATGC] # 191 525 2038 # # Bsp1407I: # [TGTACA] # 1257 1497 # # BspCNI: # [CTCAG] # 39 93 171 777 1674 # # BspHI: # [TCATGA] # 663 2091 # # BspMII*: # [TCCGGA] # 1012 1742 # # BsrBI: # [CCGCTC] # 40 2714 # # BsrDI: # [GCAATG] # 285 1149 1320 # # BsrI: # [ACTGG] # 116 119 2519 # # BssKI: # [CCNGG] # 166 180 479 823 1106 # # BssNAI: # [GTATAC] # 989 1765 # # BstEII: # [GGTNACC] # 289 2465 # # BstKTI: # [GATC] # 28 103 356 392 420 1455 # # Cac8I: # [GCNNGC] # 199 284 587 1684 # # CfrI: # [YGGCCR] # 594 2160 # # ClaI: # [ATCGAT] # 85 822 1847 # # Csp6I: # [GTAC] # 48 55 71 90 120 133 # 147 151 411 731 797 # # CviAII: # [CATG] # 240 422 590 1502 # # CviJI: # [RGCY] # 4 4 9 10 11 26 # 30 39 46 47 52 52 # 53 67 110 116 135 186 # 197 257 280 290 345 388 # # CviRI*: # [TGCA] # 127 209 236 249 257 265 # 589 822 # # DdeI: # [CTNAG] # 18 28 56 65 107 181 # 187 226 785 1101 # # DpnI: # [GATC] # 28 103 357 392 420 1454 # # Eco57I: # [CTGAAG] # 226 1140 1388 # # Eco57MI: # [CTGRAG] # 226 1140 1388 # # EcoP15I: # [CAGCAG] # 1045 1709 # # EcoRI: # [GAATTC] # 1004 1750 # # EcoRII: # [CCWGG] # 166 180 479 823 1106 # # EcoRV: # [GATATC] # 1243 1511 # # EcoT22I: # [ATGCAT] # 718 2036 # # Esp3I: # [CGTCTC] # 1136 1618 # # FalI: # [AAGNNNNNCTT] # 0 0 32 602 2120 # # FatI: # [CATG] # 240 423 589 1502 # # FauI: # [CCCGC] # 817 1937 # # FnuDII*: # [CGCG] # 828 1926 # # FokI: # [GGATG] # 335 1114 1305 # # GlaI: # [GCGC] # 485 662 778 829 # # HaeIII: # [GGCC] # 73 163 433 485 1600 # # HgaI: # [GACGC] # 61 1300 1393 # # HgiAI*: # [GWGCWC] # 1069 1685 # # HgiCI*: # [GGYRCC] # 70 564 2120 # # HgiJII*: # [GRGCYC] # 425 2329 # # HhaI: # [GCGC] # 485 663 778 828 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 4 32 # 32 32 32 324 370 870 # 1058 # # Hin4II*: # [CCTTC] # 93 99 161 218 314 329 # 1540 # # Hin6I: # [GCGC] # 485 661 778 830 # # HindII: # [GTYRAC] # 477 519 1758 # # HindIII: # [AAGCTT] # 504 850 1400 # # HinfI: # [GANTC] # 7 9 9 24 34 51 # 54 104 125 191 200 281 # 282 357 461 565 # # HpaII: # [CCGG] # 1013 1741 # # HphI: # [GGTGA] # 28 36 159 197 220 239 # 332 365 445 733 # # Hpy166II: # [GTNNAC] # 7 75 248 271 456 470 # 1227 # # Hpy178III*: # [TCNNGA] # 12 57 57 57 66 80 # 114 202 235 235 236 273 # 304 311 515 # # Hpy188I: # [TCNGA] # 13 17 30 31 155 162 # 196 309 447 470 924 # # KpnI: # [GGTACC] # 74 2680 # # Ksp632I*: # [CTCTTC] # 276 412 2066 # # MaeI: # [CTAG] # 103 106 145 258 260 494 # 1388 # # MaeII: # [ACGT] # 121 467 523 532 1111 # # MaeIII: # [GTNAC] # 34 66 111 138 255 2150 # # MboI: # [GATC] # 28 103 359 392 420 1452 # # MboII: # [GAAGA] # 3 3 9 19 32 114 # 139 173 175 220 254 267 # 277 312 316 441 # # McrI*: # [CGRYCG] # 385 2369 # # MlyI: # [GAGTC] # 45 2709 # # MmeI: # [TCCRAC] # 370 731 1653 # # MnlI: # [CCTC] # 3 3 11 15 21 84 # 93 98 119 133 150 154 # 154 164 202 213 305 343 # 489 # # MseI: # [TTAA] # 7 30 74 78 82 98 # 222 291 297 503 531 541 # # MslI: # [CAYNNNNRTG] # 1066 1688 # # MwoI: # [GCNNNNNNNGC] # 111 943 1700 # # NdeI: # [CATATG] # 16 2738 # # NlaIII: # [CATG] # 240 419 593 1502 # # NlaIV: # [GGNNCC] # 72 100 464 2118 # # NspBII*: # [CMGCKG] # 1128 1626 # # NspI: # [RCATGY] # 593 2161 # # PasI: # [CCCWGGG] # 990 1764 # # PflMI: # [CCANNNNNTGG] # 478 2276 # # PfoI: # [TCCNGGA] # 479 1106 1169 # # PleI: # [GAGTC] # 46 2708 # # PsiI: # [TTATAA] # 307 862 1585 # # PsrI: # [GAACNNNNNNTAC] # 32 501 2221 # # RsaI: # [GTAC] # 48 55 72 90 120 133 # 147 150 411 731 797 # # SalI: # [GTCGAC] # 998 1756 # # ScaI: # [AGTACT] # 120 343 1002 1289 # # ScrFI: # [CCNGG] # 168 180 477 823 1106 # # SduI: # [GDGCHC] # 425 573 687 1069 # # SecI*: # [CCNNGG] # 1 34 57 427 471 1764 # # SetI: # [ASST] # 8 13 15 15 17 19 # 21 29 30 31 32 33 # 35 36 38 41 45 52 # 55 60 60 63 64 65 # 67 69 69 79 84 86 # 87 95 118 147 148 148 # 149 150 164 217 # # SfaNI: # [GCATC] # 239 357 2158 # # SfeI*: # [CTRYAG] # 14 66 450 1029 1195 # # SmlI: # [CTYRAG] # 57 287 493 600 1317 # # SpeI: # [ACTAGT] # 495 2259 # # SspI: # [AATATT] # 183 483 2088 # # StyI: # [CCWWGG] # 34 427 471 1822 # # TaiI: # [ACGT] # 121 467 520 535 1111 # # TaqI: # [TCGA] # 24 29 55 56 88 90 # 101 103 164 196 226 304 # 1318 # # TaqII: # [CACCCA] # 81 216 258 371 1828 # # TatI: # [WGTACW] # 90 118 207 253 797 1289 # # TauI: # [GCSGC] # 1128 1626 # # TfiI: # [GAWTC] # 7 9 9 24 51 88 # 104 125 191 200 281 282 # 357 461 565 # # TseI: # [GCWGC] # 922 1832 # # TsoI: # [TARCCA] # 60 132 180 287 768 1327 # # Tsp45I: # [GTSAC] # 66 255 283 2150 # # Tsp4CI*: # [ACNGT] # 7 21 26 26 57 66 # 94 114 133 193 201 289 # 345 1182 # # TspDTI: # [ATGAA] # 6 6 11 17 28 29 # 45 48 49 49 54 72 # 73 107 108 296 433 1323 # # TspEI: # [AATT] # 13 13 13 22 31 34 # 38 38 42 46 54 57 # 66 68 68 79 88 106 # 126 137 159 174 198 249 # 330 505 # # TspGWI: # [ACGGA] # 169 829 1756 # # TspRI: # [CASTGNN] # 97 158 252 327 1920 # # XhoI: # [CTCGAG] # 57 2697 # # XhoII: # [RGATCY] # 1302 1452 # # XmnI: # [GAANNNNTTC] # 199 667 922 966 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2754 # Reported_sequences: 1 # Reported_hitcount: 574 #---------------------------------------