Restriction Fragments for MAK21/YDR060W



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The currently selected gene/sequence is :

MAK21/YDR060W on chromosome IV from coordinates 570649 to 573726.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26933.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 14 18 + BceAI ACGGC 30 32 . . 22 27 + NspBII* CMGCKG 24 24 . . 22 25 + AciI CCGC 22 24 . . 34 38 - MboII GAAGA 26 25 . . 33 38 - BbvII* GAAGAC 26 30 . . 40 44 + DdeI CTNAG 40 43 . . 62 65 + TspEI AATT 61 65 . . 68 72 - BsmAI GTCTC 62 66 . . 90 94 + MboII GAAGA 102 101 . . 113 116 + TaiI ACGT 116 112 . . 113 116 + SetI ASST 116 112 . . 113 116 + MaeII ACGT 113 115 . . 112 117 - BtrI CACGTC 114 114 . . 120 123 + CviJI RGCY 121 121 . . 120 123 + HaeIII GGCC 121 121 . . 117 121 - Hin4II* CCTTC 111 110 . . 122 126 - BsrI ACTGG 122 120 . . 130 133 + TspEI AATT 129 133 . . 140 143 + SetI ASST 143 139 . . 138 142 - Hin4II* CCTTC 132 131 . . 148 151 - MnlI CCTC 141 140 . . 151 160 + PshAI GACNNNNGTC 155 155 . . 156 159 + TaiI ACGT 159 155 . . 156 159 + SetI ASST 159 155 . . 156 159 + MaeII ACGT 156 158 . . 157 161 + Hpy99I CGWCG 161 156 . . 159 164 + Hpy178III* TCNNGA 160 162 . . 169 172 + MaeI CTAG 169 171 . . 172 182 + MwoI GCNNNNNNNGC 178 175 . . 172 182 + BstAPI GCANNNNNTGC 178 175 . . 178 183 + NlaIV GGNNCC 180 180 . . 178 183 + HgiCI* GGYRCC 178 182 . . 176 181 - TaqII CACCCA 166 164 . . 181 186 + Cac8I GCNNGC 183 183 . . 187 191 + MboII GAAGA 199 198 . . 190 194 + MboII GAAGA 202 201 . . 192 197 + XhoII RGATCY 192 196 . . 192 197 + BglII AGATCT 192 196 . . 193 196 + DpnI GATC 194 194 . . 193 196 + BstKTI GATC 195 193 . . 193 196 + MboI GATC 192 196 . . 200 203 + TspEI AATT 199 203 . . 204 208 + TfiI GAWTC 204 207 . . 204 208 + HinfI GANTC 204 207 . . 214 217 + MseI TTAA 214 216 . . 218 223 + BtgZI GCGATG 233 237 . . 229 233 + HgaI GACGC 238 243 . . 233 237 + DdeI CTNAG 233 236 . . 247 252 + ApoI RAATTY 247 251 . . 248 251 + TspEI AATT 247 251 . . 253 257 - SfaNI GCATC 243 247 . . 258 262 + Hpy99I CGWCG 262 257 . . 256 260 - BceAI ACGGC 241 243 . . 270 274 + FokI GGATG 283 287 . . 270 274 + BseGI GGATG 276 274 . . 268 271 - MnlI CCTC 261 260 . . 271 275 - SfaNI GCATC 261 265 . . 287 291 + TfiI GAWTC 287 290 . . 287 291 + HinfI GANTC 287 290 . . 290 295 + Hpy178III* TCNNGA 291 293 . . 298 301 + DpnI GATC 299 299 . . 298 301 + BstKTI GATC 300 298 . . 298 301 + MboI GATC 297 301 . . 300 304 - MboII GAAGA 292 291 . . 306 311 + ApoI RAATTY 306 310 . . 307 310 + TspEI AATT 306 310 . . 311 316 + Hpy178III* TCNNGA 312 314 . . 311 316 + BspHI TCATGA 311 315 . . 312 315 + FatI CATG 311 315 . . 312 315 + NlaIII CATG 315 311 . . 312 315 + CviAII CATG 312 314 . . 313 317 + TspDTI ATGAA 328 326 . . 310 314 - TspDTI ATGAA 300 298 . . 315 319 + MboII GAAGA 327 326 . . 344 347 + TaqI TCGA 344 346 . . 346 350 + MboII GAAGA 358 357 . . 352 357 + Hpy166II GTNNAC 354 354 . . 352 357 + AccI GTMKAC 353 355 . . 370 374 + MboII GAAGA 382 381 . . 373 377 + MboII GAAGA 385 384 . . 376 380 + TfiI GAWTC 376 379 . . 376 380 + HinfI GANTC 376 379 . . 383 386 + MaeI CTAG 383 385 . . 385 388 + SetI ASST 388 384 . . 385 388 + CviJI RGCY 386 386 . . 385 388 + AluI AGCT 386 386 . . 394 398 + TfiI GAWTC 394 397 . . 394 398 + HinfI GANTC 394 397 . . 406 410 + FauI CCCGC 414 416 . . 407 410 + AciI CCGC 407 409 . . 407 412 + BsrBI CCGCTC 409 409 . . 410 415 + SmlI CTYRAG 410 414 . . 411 416 + Hpy178III* TCNNGA 412 414 . . 410 415 - Bce83I* CTTGAG 395 393 . . 417 421 - Hin4II* CCTTC 411 410 . . 423 428 + NspI RCATGY 427 423 . . 424 427 + FatI CATG 423 427 . . 424 427 + NlaIII CATG 427 423 . . 424 427 + CviAII CATG 424 426 . . 426 429 + CviRI* TGCA 427 427 . . 441 444 + CviRI* TGCA 442 442 . . 442 446 + SfaNI GCATC 451 455 . . 438 443 - BsrDI GCAATG 437 435 . . 448 451 + TaqI TCGA 448 450 . . 457 467 + BplI GAGNNNNNCTC 448 443 480 475 463 466 + SetI ASST 466 462 . . 464 467 + MnlI CCTC 474 473 . . 457 467 - BplI GAGNNNNNCTC 480 475 448 443 472 476 + PleI GAGTC 480 481 . . 472 476 + MlyI GAGTC 481 481 . . 472 476 + HinfI GANTC 472 475 . . 478 483 + ClaI ATCGAT 479 481 . . 479 482 + TaqI TCGA 479 481 . . 488 493 + BtsI GCAGTG 495 493 . . 489 495 + TspRI CASTGNN 495 486 . . 491 496 + Hpy166II GTNNAC 493 493 . . 499 503 + MboII GAAGA 511 510 . . 508 512 + Tsp4CI* ACNGT 510 509 . . 514 518 + MboII GAAGA 526 525 . . 519 522 + CviJI RGCY 520 520 . . 514 519 - Ksp632I* CTCTTC 509 512 . . 517 520 - MnlI CCTC 510 509 . . 526 530 + DdeI CTNAG 526 529 . . 532 536 + Hpy188I TCNGA 534 533 . . 542 545 + CviJI RGCY 543 543 . . 547 551 + TfiI GAWTC 547 550 . . 547 551 + HinfI GANTC 547 550 . . 550 554 + Hpy188I TCNGA 552 551 . . 541 551 - Hin4I GAYNNNNNVTC 564 559 532 527 551 559 + AlwNI CAGNNNCTG 556 553 . . 553 557 + TfiI GAWTC 553 556 . . 553 557 + HinfI GANTC 553 556 . . 557 562 + PstI CTGCAG 561 557 . . 557 562 + SfeI* CTRYAG 557 561 . . 558 561 + CviRI* TGCA 559 559 . . 577 582 + BseRI GAGGAG 592 590 . . 577 580 - MnlI CCTC 570 569 . . 586 590 - BccI CCATC 580 581 . . 591 594 + SetI ASST 594 590 . . 590 593 - MnlI CCTC 583 582 . . 595 598 + MseI TTAA 595 597 . . 597 600 + TspEI AATT 596 600 . . 622 626 + Hpy188I TCNGA 624 623 . . 652 657 + DsaI* CCRYGG 652 656 . . 652 657 + StyI CCWWGG 652 656 . . 652 657 + NcoI CCATGG 652 656 . . 652 657 + SecI* CCNNGG 652 656 . . 653 656 + FatI CATG 652 656 . . 653 656 + NlaIII CATG 656 652 . . 653 656 + CviAII CATG 653 655 . . 659 663 + TspDTI ATGAA 674 672 . . 662 667 + ApoI RAATTY 662 666 . . 663 666 + TspEI AATT 662 666 . . 672 677 + XhoII RGATCY 672 676 . . 672 677 + NlaIV GGNNCC 674 674 . . 672 677 + BamHI GGATCC 672 676 . . 672 676 + BinI* GGATC 680 681 . . 673 676 + DpnI GATC 674 674 . . 673 676 + BstKTI GATC 675 673 . . 673 676 + MboI GATC 672 676 . . 676 679 + MnlI CCTC 686 685 . . 676 686 + BsiYI* CCNNNNNNNGG 682 679 . . 677 682 + SmlI CTYRAG 677 681 . . 673 677 - BinI* GGATC 667 668 . . 677 682 - Bce83I* CTTGAG 662 660 . . 682 687 - MmeI TCCRAC 663 661 . . 703 707 + MboII GAAGA 715 714 . . 707 710 + TspEI AATT 706 710 . . 713 718 + StyI CCWWGG 713 717 . . 713 718 + SecI* CCNNGG 713 717 . . 744 747 + SetI ASST 747 743 . . 743 746 - MnlI CCTC 736 735 . . 757 760 + MaeI CTAG 757 759 . . 762 765 + SetI ASST 765 761 . . 762 765 + CviJI RGCY 763 763 . . 762 765 + AluI AGCT 763 763 . . 784 789 + BseRI GAGGAG 799 797 . . 784 787 - MnlI CCTC 777 776 . . 789 794 + Hpy166II GTNNAC 791 791 . . 799 803 + HinfI GANTC 799 802 . . 799 803 - PleI GAGTC 793 794 . . 799 803 - MlyI GAGTC 793 793 . . 807 812 + Cac8I GCNNGC 809 809 . . 803 808 - BtgZI GCGATG 788 792 . . 810 813 + CviJI RGCY 811 811 . . 810 813 + HaeIII GGCC 811 811 . . 819 822 + FatI CATG 818 822 . . 819 822 + NlaIII CATG 822 818 . . 819 822 + CviAII CATG 819 821 . . 817 821 - TspDTI ATGAA 807 805 . . 822 826 - BslFI GGGAC 807 811 . . 835 839 + Hpy188I TCNGA 837 836 . . 839 843 + Tsp4CI* ACNGT 841 840 . . 841 846 + KpnI GGTACC 845 841 . . 841 846 + NlaIV GGNNCC 843 843 . . 841 846 + HgiCI* GGYRCC 841 845 . . 841 846 + Acc65I GGTACC 841 845 . . 842 845 + Csp6I GTAC 842 844 . . 842 845 + RsaI GTAC 843 843 . . 846 849 + MnlI CCTC 856 855 . . 853 863 + Hin4I GAYNNNNNVTC 844 839 876 871 853 863 - Hin4I GAYNNNNNVTC 876 871 844 839 868 872 + MaeIII GTNAC 867 872 . . 865 869 - BceAI ACGGC 850 852 . . 882 887 + Hpy178III* TCNNGA 883 885 . . 886 890 + TfiI GAWTC 886 889 . . 886 890 + HinfI GANTC 886 889 . . 896 899 + CviRI* TGCA 897 897 . . 893 898 - BsrDI GCAATG 892 890 . . 905 909 + DdeI CTNAG 905 908 . . 927 931 - TspDTI ATGAA 917 915 . . 946 949 + TaqI TCGA 946 948 . . 946 951 + Hpy178III* TCNNGA 947 949 . . 968 975 + PmeI GTTTAAAC 971 971 . . 969 974 + AhaIII* TTTAAA 971 971 . . 970 973 + MseI TTAA 970 972 . . 981 985 - Hin4II* CCTTC 975 974 . . 994 997 + MseI TTAA 994 996 . . 1019 1022 + SetI ASST 1022 1018 . . 1019 1022 + CviJI RGCY 1020 1020 . . 1019 1022 + AluI AGCT 1020 1020 . . 1021 1025 + DdeI CTNAG 1021 1024 . . 1022 1033 + BdaI TGANNNNNNTCA 1011 1009 1045 1043 1024 1027 + SetI ASST 1027 1023 . . 1021 1025 - BspCNI CTCAG 1013 1011 . . 1021 1025 - BseMII CTCAG 1012 1010 . . 1023 1026 - MnlI CCTC 1016 1015 . . 1020 1026 - Bpu10I CCTNAGC 1021 1024 . . 1020 1026 - BbvCI CCTCAGC 1021 1024 . . 1022 1033 - BdaI TGANNNNNNTCA 1045 1043 1011 1009 1041 1044 + SetI ASST 1044 1040 . . 1042 1046 + BssKI CCNGG 1041 1046 . . 1042 1046 + BseBI CCWGG 1043 1044 . . 1042 1046 + ScrFI CCNGG 1043 1044 . . 1042 1046 + EcoRII CCWGG 1041 1046 . . 1045 1048 + CviJI RGCY 1046 1046 . . 1056 1060 - SfaNI GCATC 1046 1050 . . 1077 1080 + CviRI* TGCA 1078 1078 . . 1080 1083 + TspEI AATT 1079 1083 . . 1090 1095 + AsuII TTCGAA 1091 1093 . . 1091 1094 + TaqI TCGA 1091 1093 . . 1093 1097 + MboII GAAGA 1105 1104 . . 1101 1106 + AhaIII* TTTAAA 1103 1103 . . 1102 1105 + MseI TTAA 1102 1104 . . 1107 1116 + XmnI GAANNNNTTC 1111 1111 . . 1110 1114 + Tsp4CI* ACNGT 1112 1111 . . 1153 1156 + DpnI GATC 1154 1154 . . 1153 1156 + BstKTI GATC 1155 1153 . . 1153 1156 + MboI GATC 1152 1156 . . 1153 1157 - BinI* GGATC 1147 1148 . . 1158 1161 + TspEI AATT 1157 1161 . . 1166 1169 + TaiI ACGT 1169 1165 . . 1166 1169 + SetI ASST 1169 1165 . . 1166 1169 + MaeII ACGT 1166 1168 . . 1179 1184 + SspI AATATT 1181 1181 . . 1183 1186 + MseI TTAA 1183 1185 . . 1189 1192 + FatI CATG 1188 1192 . . 1189 1192 + NlaIII CATG 1192 1188 . . 1189 1192 + CviAII CATG 1189 1191 . . 1225 1230 + ApoI RAATTY 1225 1229 . . 1226 1229 + TspEI AATT 1225 1229 . . 1229 1232 + MseI TTAA 1229 1231 . . 1231 1234 + TspEI AATT 1230 1234 . . 1245 1248 + SetI ASST 1248 1244 . . 1257 1260 + TspEI AATT 1256 1260 . . 1261 1265 + HphI GGTGA 1273 1272 . . 1270 1274 + TfiI GAWTC 1270 1273 . . 1270 1274 + HinfI GANTC 1270 1273 . . 1284 1289 + AsuII TTCGAA 1285 1287 . . 1285 1288 + TaqI TCGA 1285 1287 . . 1282 1286 - MboII GAAGA 1274 1273 . . 1290 1295 + AcyI GRCGYC 1291 1293 . . 1287 1291 - Hin4II* CCTTC 1281 1280 . . 1291 1295 - HgaI GACGC 1280 1285 . . 1306 1311 + Eco57MI CTGRAG 1327 1325 . . 1306 1311 + Eco57I CTGAAG 1327 1325 . . 1311 1316 - MmeI TCCRAC 1292 1290 . . 1320 1323 + SetI ASST 1323 1319 . . 1320 1323 + CviJI RGCY 1321 1321 . . 1320 1323 + AluI AGCT 1321 1321 . . 1326 1330 + Hpy188I TCNGA 1328 1327 . . 1324 1328 - FokI GGATG 1310 1314 . . 1324 1328 - BseGI GGATG 1323 1321 . . 1333 1337 + TspDTI ATGAA 1348 1346 . . 1351 1355 - SfaNI GCATC 1341 1345 . . 1363 1368 + EcoRV GATATC 1365 1365 . . 1376 1381 + TaqII GACCGA 1392 1390 . . 1374 1378 - BsmAI GTCTC 1368 1372 . . 1374 1379 - Eco31I GGTCTC 1368 1372 . . 1380 1385 + BsmI GAATGC 1386 1384 . . 1384 1387 - AciI CCGC 1384 1386 . . 1419 1424 + AhaIII* TTTAAA 1421 1421 . . 1420 1423 + MseI TTAA 1420 1422 . . 1430 1434 + BccI CCATC 1438 1439 . . 1435 1438 + MseI TTAA 1435 1437 . . 1442 1445 + DpnI GATC 1443 1443 . . 1442 1445 + BstKTI GATC 1444 1442 . . 1442 1445 + MboI GATC 1441 1445 . . 1444 1448 + Hpy188I TCNGA 1446 1445 . . 1447 1451 + MboII GAAGA 1459 1458 . . 1447 1452 + BbvII* GAAGAC 1454 1458 . . 1450 1454 + HinfI GANTC 1450 1453 . . 1450 1454 - PleI GAGTC 1444 1445 . . 1450 1454 - MlyI GAGTC 1444 1444 . . 1466 1469 + TspEI AATT 1465 1469 . . 1498 1503 + ApoI RAATTY 1498 1502 . . 1499 1502 + TspEI AATT 1498 1502 . . 1502 1507 + Hpy178III* TCNNGA 1503 1505 . . 1507 1512 + VspI ATTAAT 1508 1510 . . 1508 1511 + MseI TTAA 1508 1510 . . 1541 1544 + MseI TTAA 1541 1543 . . 1547 1551 + MaeIII GTNAC 1546 1551 . . 1564 1569 + BseRI GAGGAG 1579 1577 . . 1564 1567 - MnlI CCTC 1557 1556 . . 1576 1586 + FalI AAGNNNNNCTT 1567 1562 1599 1594 1578 1582 + MboII GAAGA 1590 1589 . . 1585 1590 + AhaIII* TTTAAA 1587 1587 . . 1586 1589 + MseI TTAA 1586 1588 . . 1576 1586 - FalI AAGNNNNNCTT 1599 1594 1567 1562 1595 1600 + Hpy166II GTNNAC 1597 1597 . . 1601 1605 + Tsp4CI* ACNGT 1603 1602 . . 1622 1625 + TaqI TCGA 1622 1624 . . 1630 1634 + TspGWI ACGGA 1645 1643 . . 1637 1641 + TspDTI ATGAA 1652 1650 . . 1649 1653 + TspDTI ATGAA 1664 1662 . . 1664 1667 + TspEI AATT 1663 1667 . . 1671 1677 + TspRI CASTGNN 1677 1668 . . 1680 1685 + Hpy166II GTNNAC 1682 1682 . . 1680 1685 + HpaI GTTAAC 1682 1682 . . 1680 1685 + HindII GTYRAC 1682 1682 . . 1681 1684 + MseI TTAA 1681 1683 . . 1686 1689 + SetI ASST 1689 1685 . . 1698 1701 + CviRI* TGCA 1699 1699 . . 1699 1704 - BsmI GAATGC 1699 1697 . . 1712 1716 + DdeI CTNAG 1712 1715 . . 1712 1716 + BspCNI CTCAG 1725 1723 . . 1712 1716 + BseMII CTCAG 1726 1724 . . 1713 1717 + Hpy188I TCNGA 1715 1714 . . 1716 1720 + TfiI GAWTC 1716 1719 . . 1716 1720 + HinfI GANTC 1716 1719 . . 1721 1729 + AlwNI CAGNNNCTG 1726 1723 . . 1723 1727 - HgaI GACGC 1712 1717 . . 1733 1737 + TspDTI ATGAA 1748 1746 . . 1737 1742 + TatI WGTACW 1737 1741 . . 1738 1741 + Csp6I GTAC 1738 1740 . . 1738 1741 + RsaI GTAC 1739 1739 . . 1741 1746 + NdeI CATATG 1742 1744 . . 1772 1775 + MnlI CCTC 1782 1781 . . 1770 1775 - BseRI GAGGAG 1761 1759 . . 1781 1784 + MseI TTAA 1781 1783 . . 1786 1789 + SetI ASST 1789 1785 . . 1787 1790 + MnlI CCTC 1797 1796 . . 1810 1815 + VspI ATTAAT 1811 1813 . . 1811 1814 + MseI TTAA 1811 1813 . . 1819 1823 + Tsp45I GTSAC 1818 1823 . . 1819 1823 + MaeIII GTNAC 1818 1823 . . 1822 1826 + Tsp4CI* ACNGT 1824 1823 . . 1820 1824 - HphI GGTGA 1812 1811 . . 1841 1845 + Tsp4CI* ACNGT 1843 1842 . . 1842 1848 + TspRI CASTGNN 1848 1839 . . 1846 1849 + DpnI GATC 1847 1847 . . 1846 1849 + BstKTI GATC 1848 1846 . . 1846 1849 + MboI GATC 1845 1849 . . 1847 1852 + ClaI ATCGAT 1848 1850 . . 1848 1851 + TaqI TCGA 1848 1850 . . 1884 1894 + BsiYI* CCNNNNNNNGG 1890 1887 . . 1890 1894 + BsrI ACTGG 1895 1893 . . 1893 1897 + HphI GGTGA 1905 1904 . . 1894 1899 + Hpy166II GTNNAC 1896 1896 . . 1899 1904 + Ksp632I* CTCTTC 1905 1908 . . 1900 1904 - MboII GAAGA 1892 1891 . . 1904 1908 + DdeI CTNAG 1904 1907 . . 1923 1928 + ApoI RAATTY 1923 1927 . . 1924 1927 + TspEI AATT 1923 1927 . . 1942 1945 + MseI TTAA 1942 1944 . . 1953 1956 + CviRI* TGCA 1954 1954 . . 1951 1955 - SfaNI GCATC 1941 1945 . . 1960 1965 + AclI AACGTT 1961 1963 . . 1961 1964 + TaiI ACGT 1964 1960 . . 1961 1964 + SetI ASST 1964 1960 . . 1961 1964 + MaeII ACGT 1961 1963 . . 1977 1980 + CviJI RGCY 1978 1978 . . 1985 1990 + Hpy178III* TCNNGA 1986 1988 . . 1991 1996 + EcoRI GAATTC 1991 1995 . . 1991 1996 + ApoI RAATTY 1991 1995 . . 1992 1995 + TspEI AATT 1991 1995 . . 1995 1999 - MboII GAAGA 1987 1986 . . 2021 2024 + TaiI ACGT 2024 2020 . . 2021 2024 + SetI ASST 2024 2020 . . 2021 2024 + MaeII ACGT 2021 2023 . . 2022 2026 + Hpy99I CGWCG 2026 2021 . . 2026 2031 + NlaIV GGNNCC 2028 2028 . . 2026 2031 + HgiCI* GGYRCC 2026 2030 . . 2035 2039 + BsrI ACTGG 2040 2038 . . 2045 2049 - MboII GAAGA 2037 2036 . . 2052 2057 + VspI ATTAAT 2053 2055 . . 2053 2056 + MseI TTAA 2053 2055 . . 2058 2062 - BsrI ACTGG 2058 2056 . . 2064 2067 + CviJI RGCY 2065 2065 . . 2063 2068 - TsoI TARCCA 2053 2051 . . 2071 2075 + Tsp4CI* ACNGT 2073 2072 . . 2091 2095 - MboII GAAGA 2083 2082 . . 2096 2101 + TsoI TARCCA 2112 2110 . . 2107 2111 - BsrI ACTGG 2107 2105 . . 2123 2127 + TspDTI ATGAA 2138 2136 . . 2125 2129 + TfiI GAWTC 2125 2128 . . 2125 2129 + HinfI GANTC 2125 2128 . . 2128 2132 + Hpy188I TCNGA 2130 2129 . . 2119 2129 - Hin4I GAYNNNNNVTC 2142 2137 2110 2105 2133 2136 + CviRI* TGCA 2134 2134 . . 2131 2135 - SfaNI GCATC 2121 2125 . . 2137 2141 + MboII GAAGA 2149 2148 . . 2140 2145 + BseRI GAGGAG 2155 2153 . . 2137 2142 - Ksp632I* CTCTTC 2132 2135 . . 2140 2143 - MnlI CCTC 2133 2132 . . 2150 2154 + BslFI GGGAC 2164 2168 . . 2169 2173 - Hin4II* CCTTC 2163 2162 . . 2178 2182 + Hpy99I CGWCG 2182 2177 . . 2180 2184 + BceAI ACGGC 2196 2198 . . 2181 2186 + McrI* CGRYCG 2184 2182 . . 2181 2186 + XmaIII* CGGCCG 2181 2185 . . 2181 2186 + CfrI YGGCCR 2181 2185 . . 2182 2185 + CviJI RGCY 2183 2183 . . 2182 2185 + HaeIII GGCC 2183 2183 . . 2183 2187 + TauI GCSGC 2186 2183 . . 2183 2187 + BlsI GCNGC 2185 2184 . . 2183 2187 + BisI GCNGC 2184 2185 . . 2184 2187 + AciI CCGC 2184 2186 . . 2186 2191 + Cac8I GCNNGC 2188 2188 . . 2194 2197 + DpnI GATC 2195 2195 . . 2194 2197 + BstKTI GATC 2196 2194 . . 2194 2197 + MboI GATC 2193 2197 . . 2194 2198 - BinI* GGATC 2188 2189 . . 2221 2225 - MboII GAAGA 2213 2212 . . 2245 2249 - TspDTI ATGAA 2235 2233 . . 2258 2262 - TspDTI ATGAA 2248 2246 . . 2260 2264 - FokI GGATG 2246 2250 . . 2260 2264 - BseGI GGATG 2259 2257 . . 2266 2270 + Tsp4CI* ACNGT 2268 2267 . . 2276 2281 + NdeI CATATG 2277 2279 . . 2280 2283 + CviRI* TGCA 2281 2281 . . 2293 2297 + Tsp45I GTSAC 2292 2297 . . 2293 2297 + MaeIII GTNAC 2292 2297 . . 2316 2319 + SetI ASST 2319 2315 . . 2316 2319 + CviJI RGCY 2317 2317 . . 2316 2319 + AluI AGCT 2317 2317 . . 2323 2333 + BsiYI* CCNNNNNNNGG 2329 2326 . . 2326 2335 + PshAI GACNNNNGTC 2330 2330 . . 2327 2330 + SetI ASST 2330 2326 . . 2328 2332 + BssKI CCNGG 2327 2332 . . 2328 2332 + BseBI CCWGG 2329 2330 . . 2328 2333 + SecI* CCNNGG 2328 2332 . . 2328 2332 + ScrFI CCNGG 2329 2330 . . 2328 2332 + EcoRII CCWGG 2327 2332 . . 2359 2370 + DrdI GACNNNNNNGTC 2365 2363 . . 2368 2373 + Hpy166II GTNNAC 2370 2370 . . 2368 2373 + AccI GTMKAC 2369 2371 . . 2370 2375 + SfeI* CTRYAG 2370 2374 . . 2403 2406 + SetI ASST 2406 2402 . . 2402 2405 - MnlI CCTC 2395 2394 . . 2405 2408 + Csp6I GTAC 2405 2407 . . 2405 2408 + RsaI GTAC 2406 2406 . . 2417 2420 + CviRI* TGCA 2418 2418 . . 2421 2424 + SetI ASST 2424 2420 . . 2422 2425 + MnlI CCTC 2432 2431 . . 2434 2437 + CviJI RGCY 2435 2435 . . 2437 2442 + Hpy178III* TCNNGA 2438 2440 . . 2438 2443 + BsiI* CACGAG 2438 2442 . . 2444 2447 + MseI TTAA 2444 2446 . . 2449 2453 + HinfI GANTC 2449 2452 . . 2449 2457 + Tth111I GACNNNGTC 2452 2453 . . 2449 2453 - PleI GAGTC 2443 2444 . . 2449 2453 - MlyI GAGTC 2443 2443 . . 2457 2461 + DdeI CTNAG 2457 2460 . . 2462 2465 + MseI TTAA 2462 2464 . . 2467 2471 + SfaNI GCATC 2476 2480 . . 2470 2474 + Hpy188I TCNGA 2472 2471 . . 2479 2484 + EcoT22I ATGCAT 2483 2479 . . 2480 2483 + CviRI* TGCA 2481 2481 . . 2482 2485 + FatI CATG 2481 2485 . . 2482 2485 + NlaIII CATG 2485 2481 . . 2482 2485 + CviAII CATG 2482 2484 . . 2485 2494 + PshAI GACNNNNGTC 2489 2489 . . 2487 2491 + BssKI CCNGG 2486 2491 . . 2487 2491 + BseBI CCWGG 2488 2489 . . 2487 2492 + SecI* CCNNGG 2487 2491 . . 2487 2491 + ScrFI CCNGG 2488 2489 . . 2487 2491 + EcoRII CCWGG 2486 2491 . . 2490 2496 + SanDI GGGWCCC 2491 2494 . . 2490 2496 + PpuMI RGGWCCY 2491 2494 . . 2490 2496 + DraII RGGNCCY 2491 2494 . . 2490 2495 + NlaIV GGNNCC 2492 2492 . . 2491 2496 + NlaIV GGNNCC 2493 2493 . . 2491 2495 + BmgT120I GGNCC 2492 2493 . . 2491 2495 + AvaII GGWCC 2491 2494 . . 2491 2495 + AsuI* GGNCC 2491 2494 . . 2492 2496 - BslFI GGGAC 2477 2481 . . 2497 2502 + Hpy166II GTNNAC 2499 2499 . . 2502 2508 + TspRI CASTGNN 2508 2499 . . 2504 2509 + Eco57MI CTGRAG 2525 2523 . . 2504 2509 + Eco57I CTGAAG 2525 2523 . . 2506 2510 + MboII GAAGA 2518 2517 . . 2506 2511 + BbvII* GAAGAC 2513 2517 . . 2510 2514 + BsrI ACTGG 2515 2513 . . 2513 2516 + CviJI RGCY 2514 2514 . . 2512 2517 - TsoI TARCCA 2502 2500 . . 2530 2534 + MboII GAAGA 2542 2541 . . 2533 2538 + EcoRV GATATC 2535 2535 . . 2541 2544 + SetI ASST 2544 2540 . . 2543 2548 + Eco57MI CTGRAG 2564 2562 . . 2543 2548 + Eco57I CTGAAG 2564 2562 . . 2545 2549 + MboII GAAGA 2557 2556 . . 2551 2556 + ApoI RAATTY 2551 2555 . . 2552 2555 + TspEI AATT 2551 2555 . . 2564 2569 + SspI AATATT 2566 2566 . . 2585 2590 + NspBII* CMGCKG 2587 2587 . . 2585 2588 + AciI CCGC 2585 2587 . . 2590 2594 - BccI CCATC 2584 2585 . . 2617 2621 + SfaNI GCATC 2626 2630 . . 2623 2626 + TspEI AATT 2622 2626 . . 2627 2630 + TaqI TCGA 2627 2629 . . 2646 2650 + FokI GGATG 2659 2663 . . 2646 2650 + BseGI GGATG 2652 2650 . . 2648 2652 + TspDTI ATGAA 2663 2661 . . 2654 2658 + TspDTI ATGAA 2669 2667 . . 2657 2662 + ApoI RAATTY 2657 2661 . . 2658 2661 + TspEI AATT 2657 2661 . . 2675 2678 + MseI TTAA 2675 2677 . . 2672 2677 - TsoI TARCCA 2662 2660 . . 2680 2683 + TaqI TCGA 2680 2682 . . 2680 2685 + Hpy178III* TCNNGA 2681 2683 . . 2682 2686 - BsmAI GTCTC 2676 2680 . . 2682 2687 - Eco31I GGTCTC 2676 2680 . . 2695 2699 + MboII GAAGA 2707 2706 . . 2706 2710 + Hpy99I CGWCG 2710 2705 . . 2711 2715 + Tsp4CI* ACNGT 2713 2712 . . 2712 2718 + TspRI CASTGNN 2718 2709 . . 2714 2719 + Hpy166II GTNNAC 2716 2716 . . 2729 2734 + Eco57MI CTGRAG 2750 2748 . . 2729 2734 + Eco57I CTGAAG 2750 2748 . . 2731 2735 + MboII GAAGA 2743 2742 . . 2746 2750 + TfiI GAWTC 2746 2749 . . 2746 2750 + HinfI GANTC 2746 2749 . . 2752 2757 + SpeI ACTAGT 2752 2756 . . 2753 2756 + MaeI CTAG 2753 2755 . . 2756 2760 + Tsp45I GTSAC 2755 2760 . . 2756 2760 + MaeIII GTNAC 2755 2760 . . 2762 2766 + TspDTI ATGAA 2777 2775 . . 2766 2769 + SetI ASST 2769 2765 . . 2767 2773 + Bpu10I CCTNAGC 2768 2771 . . 2768 2772 + DdeI CTNAG 2768 2771 . . 2771 2774 + SetI ASST 2774 2770 . . 2771 2774 + CviJI RGCY 2772 2772 . . 2771 2774 + AluI AGCT 2772 2772 . . 2776 2780 + HgaI GACGC 2785 2790 . . 2780 2785 + MfeI CAATTG 2780 2784 . . 2781 2784 + TspEI AATT 2780 2784 . . 2789 2793 + TspDTI ATGAA 2804 2802 . . 2791 2795 + MboII GAAGA 2803 2802 . . 2794 2798 - SfaNI GCATC 2784 2788 . . 2806 2810 - Hin4II* CCTTC 2800 2799 . . 2810 2814 + BlsI GCNGC 2812 2811 . . 2810 2814 + BisI GCNGC 2811 2812 . . 2810 2814 + BbvI GCAGC 2822 2826 . . 2810 2814 + TseI GCWGC 2810 2813 . . 2812 2815 + CviJI RGCY 2813 2813 . . 2813 2823 + MwoI GCNNNNNNNGC 2819 2816 . . 2824 2827 + DpnI GATC 2825 2825 . . 2824 2827 + BstKTI GATC 2826 2824 . . 2824 2827 + MboI GATC 2823 2827 . . 2826 2831 + Hpy178III* TCNNGA 2827 2829 . . 2830 2834 + MboII GAAGA 2842 2841 . . 2835 2840 + StuI AGGCCT 2837 2837 . . 2836 2839 + CviJI RGCY 2837 2837 . . 2836 2839 + HaeIII GGCC 2837 2837 . . 2833 2837 - Hin4II* CCTTC 2827 2826 . . 2838 2841 + MnlI CCTC 2848 2847 . . 2838 2848 + EcoNI CCTNNNNNAGG 2842 2843 . . 2838 2848 + BsiYI* CCNNNNNNNGG 2844 2841 . . 2840 2843 + TaqI TCGA 2840 2842 . . 2845 2848 - MnlI CCTC 2838 2837 . . 2858 2862 + Tsp4CI* ACNGT 2860 2859 . . 2864 2867 + TaqI TCGA 2864 2866 . . 2866 2870 - BccI CCATC 2860 2861 . . 2894 2899 + AclI AACGTT 2895 2897 . . 2895 2898 + TaiI ACGT 2898 2894 . . 2895 2898 + SetI ASST 2898 2894 . . 2895 2898 + MaeII ACGT 2895 2897 . . 2900 2904 + Hin4II* CCTTC 2910 2909 . . 2917 2921 + MboII GAAGA 2929 2928 . . 2917 2922 - Ksp632I* CTCTTC 2912 2915 . . 2920 2923 - MnlI CCTC 2913 2912 . . 2930 2934 + BlsI GCNGC 2932 2931 . . 2930 2934 + BisI GCNGC 2931 2932 . . 2930 2934 + BbvI GCAGC 2942 2946 . . 2930 2934 + TseI GCWGC 2930 2933 . . 2935 2939 + MboII GAAGA 2947 2946 . . 2938 2942 + MboII GAAGA 2950 2949 . . 2947 2951 + MboII GAAGA 2959 2958 . . 2950 2954 + MboII GAAGA 2962 2961 . . 2953 2957 + MboII GAAGA 2965 2964 . . 2966 2969 + SetI ASST 2969 2965 . . 2965 2968 - MnlI CCTC 2958 2957 . . 2968 2973 + BciVI GTATCC 2979 2978 . . 2972 2975 + AciI CCGC 2972 2974 . . 2974 2984 + MwoI GCNNNNNNNGC 2980 2977 . . 2988 2992 + MboII GAAGA 3000 2999 . . 3017 3022 + Hpy166II GTNNAC 3019 3019 . . 3017 3022 + AccI GTMKAC 3018 3020 . . 3021 3026 + Cfr10I RCCGGY 3021 3025 . . 3021 3026 + BetI* WCCGGW 3021 3025 . . 3021 3026 + AgeI ACCGGT 3021 3025 . . 3022 3025 + HpaII CCGG 3022 3024 . . 3030 3033 + CviRI* TGCA 3031 3031 . . 3031 3035 + SfaNI GCATC 3040 3044 . . 3039 3043 + Hpy99I CGWCG 3043 3038 . . 3039 3050 + BcgI CGANNNNNNTGC 3028 3026 3062 3060 3053 3058 + SspI AATATT 3055 3055 . . 3061 3064 + DpnI GATC 3062 3062 . . 3061 3064 + BstKTI GATC 3063 3061 . . 3061 3064 + MboI GATC 3060 3064 . . 3063 3068 + Hpy178III* TCNNGA 3064 3066 . . 3067 3071 + TfiI GAWTC 3067 3070 . . 3067 3071 + HinfI GANTC 3067 3070 . . 3070 3074 + Hpy188I TCNGA 3072 3071 . . 3075 3078 + MaeI CTAG 3075 3077 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 841 2237 # # AccI: # [GTMKAC] # 60 353 649 2016 # # AciI: # [CCGC] # 22 106 385 387 401 800 # 977 # # AclI: # [AACGTT] # 183 934 1961 # # AcyI: # [GRCGYC] # 1291 1787 # # AgeI: # [ACCGGT] # 57 3021 # # AhaIII*: # [TTTAAA] # 132 166 318 971 1491 # # AluI: # [AGCT] # 257 301 306 377 386 455 # 996 # # AlwNI: # [CAGNNNCTG] # 556 1170 1352 # # ApoI: # [RAATTY] # 59 68 106 247 273 356 # 421 425 560 563 # # AsuI*: # [GGNCC] # 587 2491 # # AsuII: # [TTCGAA] # 194 1091 1793 # # AvaII: # [GGWCC] # 587 2491 # # BamHI: # [GGATCC] # 672 2406 # # BbvCI: # [CCTCAGC] # 1021 2057 # # BbvI: # [GCAGC] # 120 136 2822 # # BbvII*: # [GAAGAC] # 26 565 1059 1428 # # BccI: # [CCATC] # 218 276 580 858 1146 # # Bce83I*: # [CTTGAG] # 267 395 2416 # # BceAI: # [ACGGC] # 30 211 609 882 1346 # # BcgI: # [CGANNNNNNTGC] # 16 34 3028 # # BciVI: # [GTATCC] # 99 2979 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 1011 2033 # # BetI*: # [WCCGGW] # 57 3021 # # BglII: # [AGATCT] # 192 2886 # # BinI*: # [GGATC] # 13 467 667 890 1041 # # BisI: # [GCNGC] # 120 147 627 2184 # # BlsI: # [GCNGC] # 120 146 627 2185 # # BmgT120I: # [GGNCC] # 586 2492 # # BplI: # [GAGNNNNNCTC] # 0 0 32 448 2598 # # Bpu10I: # [CCTNAGC] # 310 1021 1747 # # BseBI: # [CCWGG] # 159 590 1043 1286 # # BseGI: # [GGATG] # 276 393 426 936 1047 # # BseMII: # [CTCAG] # 714 1012 1352 # # BseRI: # [GAGGAG] # 182 207 394 592 780 923 # # BsiI*: # [CACGAG] # 640 2438 # # BsiYI*: # [CCNNNNNNNGG] # 234 439 515 682 1208 # # BslFI: # [GGGAC] # 313 601 807 1357 # # BsmAI: # [GTCTC] # 62 402 1306 1308 # # BsmI: # [GAATGC] # 313 1379 1386 # # BspCNI: # [CTCAG] # 712 1013 1353 # # BspHI: # [TCATGA] # 311 2767 # # BsrBI: # [CCGCTC] # 409 2669 # # BsrDI: # [GCAATG] # 437 455 2186 # # BsrI: # [ACTGG] # 18 49 122 145 408 563 # 1773 # # BssKI: # [CCNGG] # 159 592 1041 1286 # # BstAPI: # [GCANNNNNTGC] # 178 2900 # # BstKTI: # [GATC] # 15 105 195 237 289 348 # 375 404 480 630 # # BtgZI: # [GCGATG] # 233 555 2290 # # BtrI: # [CACGTC] # 114 2964 # # BtsI: # [GCAGTG] # 495 2583 # # Cac8I: # [GCNNGC] # 183 626 890 1379 # # Cfr10I: # [RCCGGY] # 57 3021 # # CfrI: # [YGGCCR] # 897 2181 # # ClaI: # [ATCGAT] # 479 1230 1369 # # Csp6I: # [GTAC] # 667 673 842 896 # # CviAII: # [CATG] # 112 166 229 312 370 596 # 1293 # # CviJI: # [RGCY] # 23 24 26 41 48 79 # 87 118 118 121 134 134 # 209 220 241 258 265 275 # 657 # # CviRI*: # [TGCA] # 15 47 63 117 137 147 # 180 181 255 338 427 550 # 621 # # DdeI: # [CTNAG] # 40 116 192 193 293 310 # 311 379 553 691 # # DpnI: # [GATC] # 16 105 194 237 289 348 # 375 404 480 630 # # DraII: # [RGGNCCY] # 587 2491 # # DrdI: # [GACNNNNNNGTC] # 713 2365 # # DsaI*: # [CCRYGG] # 652 2426 # # Eco31I: # [GGTCTC] # 402 1308 1368 # # Eco57I: # [CTGAAG] # 39 186 328 1198 1327 # # Eco57MI: # [CTGRAG] # 39 186 328 1198 1327 # # EcoNI: # [CCTNNNNNAGG] # 236 2842 # # EcoRI: # [GAATTC] # 1087 1991 # # EcoRII: # [CCWGG] # 159 592 1041 1286 # # EcoRV: # [GATATC] # 543 1170 1365 # # EcoT22I: # [ATGCAT] # 595 2483 # # FalI: # [AAGNNNNNCTT] # 0 0 32 1479 1567 # # FatI: # [CATG] # 112 166 229 311 370 597 # 1293 # # FauI: # [CCCGC] # 414 2664 # # FokI: # [GGATG] # 283 413 419 936 1027 # # HaeIII: # [GGCC] # 121 241 654 690 1372 # # HgaI: # [GACGC] # 238 293 432 1042 1073 # # HgiCI*: # [GGYRCC] # 178 663 1052 1185 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 280 # 532 936 1234 # # Hin4II*: # [CCTTC] # 21 27 83 111 168 279 # 306 564 637 882 # # HindII: # [GTYRAC] # 1396 1682 # # HinfI: # [GANTC] # 6 11 18 75 78 83 # 87 89 180 204 246 266 # 297 321 324 384 409 # # HpaI: # [GTTAAC] # 1396 1682 # # HpaII: # [CCGG] # 56 3022 # # HphI: # [GGTGA] # 93 539 1173 1273 # # Hpy166II: # [GTNNAC] # 59 85 129 139 214 217 # 298 303 354 474 806 # # Hpy178III*: # [TCNNGA] # 14 21 64 100 131 146 # 160 237 243 452 471 483 # 556 # # Hpy188I: # [TCNGA] # 6 18 72 118 213 269 # 342 415 491 534 600 # # Hpy99I: # [CGWCG] # 35 101 156 161 333 528 # 1764 # # KpnI: # [GGTACC] # 845 2233 # # Ksp632I*: # [CTCTTC] # 166 227 509 780 1396 # # MaeI: # [CTAG] # 3 169 214 322 374 1996 # # MaeII: # [ACGT] # 43 60 113 183 795 874 # 1010 # # MaeIII: # [GTNAC] # 272 323 463 474 679 867 # # MboI: # [GATC] # 18 105 192 237 289 348 # 375 404 480 630 # # MboII: # [GAAGA] # 3 3 3 3 3 9 # 15 15 18 24 24 26 # 31 35 35 36 39 46 # 50 60 64 66 76 78 # 87 90 95 97 126 131 # 150 169 185 189 302 305 # 390 # # McrI*: # [CGRYCG] # 894 2184 # # MfeI: # [CAATTG] # 298 2780 # # MlyI: # [GAGTC] # 312 481 635 651 999 # # MmeI: # [TCCRAC] # 629 663 1786 # # MnlI: # [CCTC] # 10 13 15 36 37 41 # 45 50 60 65 79 103 # 120 120 141 160 213 225 # 262 336 406 541 # # MseI: # [TTAA] # 15 18 24 30 33 45 # 46 73 81 95 100 108 # 111 131 191 213 214 375 # 381 391 403 # # MwoI: # [GCNNNNNNNGC] # 98 161 178 2641 # # NcoI: # [CCATGG] # 652 2426 # # NdeI: # [CATATG] # 535 801 1742 # # NlaIII: # [CATG] # 112 166 229 315 370 593 # 1293 # # NlaIV: # [GGNNCC] # 1 169 180 464 494 585 # 1185 # # NspBII*: # [CMGCKG] # 24 491 2563 # # NspI: # [RCATGY] # 427 2651 # # PleI: # [GAGTC] # 313 480 635 651 999 # # PmeI: # [GTTTAAAC] # 971 2107 # # PpuMI: # [RGGWCCY] # 587 2491 # # PshAI: # [GACNNNNGTC] # 155 159 589 2175 # # PstI: # [CTGCAG] # 561 2517 # # RsaI: # [GTAC] # 667 672 843 896 # # SanDI: # [GGGWCCC] # 587 2491 # # ScrFI: # [CCNGG] # 159 590 1043 1286 # # SecI*: # [CCNNGG] # 61 159 591 652 1615 # # SetI: # [ASST] # 5 5 11 16 17 18 # 18 27 60 71 75 76 # 78 79 100 109 116 120 # 124 125 128 153 175 225 # 229 257 295 366 # # SfaNI: # [GCATC] # 18 38 150 158 180 190 # 243 256 295 355 595 600 # # SfeI*: # [CTRYAG] # 557 708 1813 # # SmlI: # [CTYRAG] # 267 410 2401 # # SpeI: # [ACTAGT] # 326 2752 # # SspI: # [AATATT] # 23 489 1181 1385 # # StuI: # [AGGCCT] # 241 2837 # # StyI: # [CCWWGG] # 61 652 2365 # # TaiI: # [ACGT] # 43 60 116 180 795 874 # 1010 # # TaqI: # [TCGA] # 24 31 53 104 145 160 # 194 214 226 337 344 467 # 779 # # TaqII: # [CACCCA] # 166 1226 1686 # # TatI: # [WGTACW] # 1341 1737 # # TauI: # [GCSGC] # 892 2186 # # TfiI: # [GAWTC] # 6 11 18 83 89 153 # 204 321 333 384 409 446 # 621 # # TseI: # [GCWGC] # 120 148 2810 # # TsoI: # [TARCCA] # 59 160 390 416 2053 # # Tsp45I: # [GTSAC] # 323 463 474 1818 # # Tsp4CI*: # [ACNGT] # 19 147 195 218 221 230 # 271 331 445 491 510 # # TspDTI: # [ATGAA] # 6 12 13 27 28 84 # 97 108 110 133 274 300 # 304 346 390 415 431 # # TspEI: # [AATT] # 5 26 33 35 44 48 # 59 61 66 68 68 68 # 70 71 78 123 165 209 # 260 290 298 373 560 # # TspGWI: # [ACGGA] # 1433 1645 # # TspRI: # [CASTGNN] # 171 210 360 495 660 1182 # # Tth111I: # [GACNNNGTC] # 626 2452 # # VspI: # [ATTAAT] # 242 303 1025 1508 # # XhoII: # [RGATCY] # 192 480 2406 # # XmaIII*: # [CGGCCG] # 897 2181 # # XmnI: # [GAANNNNTTC] # 1111 1967 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3078 # Reported_sequences: 1 # Reported_hitcount: 626 #---------------------------------------