Restriction Fragments for UBC5/YDR059C



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

UBC5/YDR059C on chromosome IV from coordinates 569770 to 569234.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15594.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 11 + MnlI CCTC 18 17 . . 23 28 + StyI CCWWGG 23 27 . . 23 28 + SecI* CCNNGG 23 27 . . 29 32 + TspEI AATT 28 32 . . 31 34 + MseI TTAA 31 33 . . 46 49 + SetI ASST 49 45 . . 45 48 - MnlI CCTC 38 37 . . 53 56 + MseI TTAA 53 55 . . 64 68 + TspDTI ATGAA 79 77 . . 76 81 + Hpy178III* TCNNGA 77 79 . . 80 83 + DpnI GATC 81 81 . . 80 83 + BstKTI GATC 82 80 . . 80 83 + MboI GATC 79 83 . . 106 109 + FatI CATG 105 109 . . 106 109 + NlaIII CATG 109 105 . . 106 109 + CviAII CATG 106 108 . . 131 135 + Hpy188I TCNGA 133 132 . . 134 138 + MboII GAAGA 146 145 . . 138 143 + XhoII RGATCY 138 142 . . 139 142 + DpnI GATC 140 140 . . 139 142 + BstKTI GATC 141 139 . . 134 139 - Ksp632I* CTCTTC 129 132 . . 139 142 + MboI GATC 138 142 . . 142 145 + MnlI CCTC 152 151 . . 139 143 - BinI* GGATC 133 134 . . 152 155 + FatI CATG 151 155 . . 152 155 + NlaIII CATG 155 151 . . 152 155 + CviAII CATG 152 154 . . 150 154 - TspDTI ATGAA 140 138 . . 158 163 + EcoP15I CAGCAG 188 190 . . 161 169 + AlwNI CAGNNNCTG 166 163 . . 162 168 + PpuMI RGGWCCY 163 166 . . 162 168 + DraII RGGNCCY 163 166 . . 163 167 + BmgT120I GGNCC 164 165 . . 163 167 + AvaII GGWCC 163 166 . . 163 167 + AsuI* GGNCC 163 166 . . 165 168 + SetI ASST 168 164 . . 167 172 + SfeI* CTRYAG 167 171 . . 174 178 + FokI GGATG 187 191 . . 174 178 + BseGI GGATG 180 178 . . 177 188 + BdaI TGANNNNNNTCA 166 164 200 198 179 182 + SetI ASST 182 178 . . 177 188 - BdaI TGANNNNNNTCA 200 198 166 164 192 197 + Cac8I GCNNGC 194 194 . . 195 198 + CviJI RGCY 196 196 . . 197 200 + MnlI CCTC 207 206 . . 207 213 + PpuMI RGGWCCY 208 211 . . 207 213 + DraII RGGNCCY 208 211 . . 207 212 + NlaIV GGNNCC 209 209 . . 208 212 + BmgT120I GGNCC 209 210 . . 208 212 + AvaII GGWCC 208 211 . . 208 212 + AsuI* GGNCC 208 211 . . 211 215 + Hin4II* CCTTC 221 220 . . 214 218 + Hpy188I TCNGA 216 215 . . 217 221 + HinfI GANTC 217 220 . . 212 217 - Eco57MI CTGRAG 197 195 . . 212 217 - Eco57I CTGAAG 197 195 . . 217 221 - PleI GAGTC 211 212 . . 217 221 - MlyI GAGTC 211 211 . . 223 233 + BsiYI* CCNNNNNNNGG 229 226 . . 220 224 - HphI GGTGA 212 211 . . 261 266 + MmeI TCCRAC 286 284 . . 281 284 + CviJI RGCY 282 282 . . 289 299 + FalI AAGNNNNNCTT 280 275 312 307 290 293 + SetI ASST 293 289 . . 288 292 - Hin4II* CCTTC 282 281 . . 292 297 + Hpy166II GTNNAC 294 294 . . 289 299 - FalI AAGNNNNNCTT 312 307 280 275 308 313 + ApoI RAATTY 308 312 . . 309 312 + TspEI AATT 308 312 . . 318 322 + Hpy188I TCNGA 320 319 . . 316 320 - FokI GGATG 302 306 . . 316 320 - BseGI GGATG 315 313 . . 322 327 + SspI AATATT 324 324 . . 325 330 + VspI ATTAAT 326 328 . . 326 329 + MseI TTAA 326 328 . . 328 331 + TspEI AATT 327 331 . . 331 334 + TaqI TCGA 331 333 . . 357 362 + AhaIII* TTTAAA 359 359 . . 358 361 + MseI TTAA 358 360 . . 363 367 + BmgT120I GGNCC 364 365 . . 363 367 + AvaII GGWCC 363 366 . . 363 367 + AsuI* GGNCC 363 366 . . 364 374 + Hin4I GAYNNNNNVTC 355 350 387 382 364 374 + Eam1105I GACNNNNNGTC 369 368 . . 367 373 + TspRI CASTGNN 373 364 . . 366 370 - BsrI ACTGG 366 364 . . 371 377 + BstEII GGTNACC 371 376 . . 372 376 + Tsp45I GTSAC 371 376 . . 372 376 + MaeIII GTNAC 371 376 . . 374 381 + SgrAI CRCCGGYG 375 379 . . 364 374 - Hin4I GAYNNNNNVTC 387 382 355 350 375 380 + Cfr10I RCCGGY 375 379 . . 376 379 + HpaII CCGG 376 378 . . 373 377 - HphI GGTGA 365 364 . . 378 383 + HaeII RGCGCY 382 378 . . 379 382 + Hin6I GCGC 379 381 . . 379 382 + GlaI GCGC 380 380 . . 379 382 + HhaI GCGC 381 379 . . 421 424 + MseI TTAA 421 423 . . 427 436 + BsaBI GATNNNNATC 431 431 . . 427 431 - SfaNI GCATC 417 421 . . 435 440 + Hpy178III* TCNNGA 436 438 . . 438 442 + Hpy99I CGWCG 442 437 . . 442 445 + DpnI GATC 443 443 . . 442 445 + BstKTI GATC 444 442 . . 442 445 + MboI GATC 441 445 . . 442 446 - BinI* GGATC 436 437 . . 450 455 + NlaIV GGNNCC 452 452 . . 450 454 + BmgT120I GGNCC 451 452 . . 450 454 + AvaII GGWCC 450 453 . . 450 454 + AsuI* GGNCC 450 453 . . 451 455 - BslFI GGGAC 436 440 . . 456 467 + BdaI TGANNNNNNTCA 445 443 479 477 459 462 + TspEI AATT 458 462 . . 456 467 - BdaI TGANNNNNNTCA 479 477 445 443 486 489 + CviJI RGCY 487 487 . . 488 492 + DdeI CTNAG 488 491 . . 494 498 + TspDTI ATGAA 509 507 . . 498 501 + CviJI RGCY 499 499 . . 503 506 + AciI CCGC 503 505 . . 506 510 + DdeI CTNAG 506 509 . . 505 511 - Bpu10I CCTNAGC 506 509 . . 513 518 + Hpy166II GTNNAC 515 515 . . #--------------------------------------- # # # AciI: # [CCGC] # 34 503 # # AhaIII*: # [TTTAAA] # 178 359 # # AlwNI: # [CAGNNNCTG] # 166 371 # # ApoI: # [RAATTY] # 229 308 # # AsuI*: # [GGNCC] # 45 87 87 155 163 # # AvaII: # [GGWCC] # 45 87 87 155 163 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 58 166 245 # # BinI*: # [GGATC] # 101 133 303 # # BmgT120I: # [GGNCC] # 45 86 87 155 164 # # Bpu10I: # [CCTNAGC] # 31 506 # # BsaBI: # [GATNNNNATC] # 106 431 # # BseGI: # [GGATG] # 135 180 222 # # BsiYI*: # [CCNNNNNNNGG] # 229 308 # # BslFI: # [GGGAC] # 101 436 # # BsrI: # [ACTGG] # 171 366 # # BstEII: # [GGTNACC] # 166 371 # # BstKTI: # [GATC] # 59 82 93 303 # # Cac8I: # [GCNNGC] # 194 343 # # Cfr10I: # [RCCGGY] # 162 375 # # CviAII: # [CATG] # 46 106 385 # # CviJI: # [RGCY] # 12 38 86 196 205 # # DdeI: # [CTNAG] # 18 31 488 # # DpnI: # [GATC] # 59 81 94 303 # # DraII: # [RGGNCCY] # 45 163 329 # # Eam1105I: # [GACNNNNNGTC] # 168 369 # # Eco57I: # [CTGAAG] # 197 340 # # Eco57MI: # [CTGRAG] # 197 340 # # EcoP15I: # [CAGCAG] # 188 349 # # FalI: # [AAGNNNNNCTT] # 0 0 32 225 280 # # FatI: # [CATG] # 46 105 386 # # FokI: # [GGATG] # 115 187 235 # # GlaI: # [GCGC] # 157 380 # # HaeII: # [RGCGCY] # 155 382 # # HhaI: # [GCGC] # 156 381 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 150 355 # # Hin4II*: # [CCTTC] # 61 221 255 # # Hin6I: # [GCGC] # 158 379 # # HinfI: # [GANTC] # 217 320 # # HpaII: # [CCGG] # 161 376 # # HphI: # [GGTGA] # 153 172 212 # # Hpy166II: # [GTNNAC] # 22 221 294 # # Hpy178III*: # [TCNNGA] # 77 101 359 # # Hpy188I: # [TCNGA] # 83 104 133 217 # # Hpy99I: # [CGWCG] # 95 442 # # Ksp632I*: # [CTCTTC] # 129 408 # # MaeIII: # [GTNAC] # 166 371 # # MboI: # [GATC] # 59 79 96 303 # # MboII: # [GAAGA] # 146 391 # # MlyI: # [GAGTC] # 211 326 # # MmeI: # [TCCRAC] # 251 286 # # MnlI: # [CCTC] # 18 20 55 114 330 # # MseI: # [TTAA] # 22 31 32 63 116 273 # # NlaIII: # [CATG] # 46 109 382 # # NlaIV: # [GGNNCC] # 85 209 243 # # PleI: # [GAGTC] # 211 326 # # PpuMI: # [RGGWCCY] # 45 163 329 # # SecI*: # [CCNNGG] # 23 514 # # SetI: # [ASST] # 14 49 111 119 244 # # SfaNI: # [GCATC] # 120 417 # # SfeI*: # [CTRYAG] # 167 370 # # SgrAI: # [CRCCGGYG] # 162 375 # # SspI: # [AATATT] # 213 324 # # StyI: # [CCWWGG] # 23 514 # # TaqI: # [TCGA] # 206 331 # # Tsp45I: # [GTSAC] # 166 371 # # TspDTI: # [ATGAA] # 28 61 79 369 # # TspEI: # [AATT] # 19 28 79 131 280 # # TspRI: # [CASTGNN] # 164 373 # # VspI: # [ATTAAT] # 211 326 # # XhoII: # [RGATCY] # 138 399 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 537 # Reported_sequences: 1 # Reported_hitcount: 124 #---------------------------------------