Restriction Fragments for REG1/YDR028C



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The currently selected gene/sequence is :

REG1/YDR028C on chromosome IV from coordinates 500879 to 497835.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.31768.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 3 8 + HindII GTYRAC 5 5 . . 13 16 + MaeI CTAG 13 15 . . 19 22 + TspEI AATT 18 22 . . 28 33 + Cfr10I RCCGGY 28 32 . . 29 32 + HpaII CCGG 29 31 . . 54 59 + NspI RCATGY 58 54 . . 55 58 + FatI CATG 54 58 . . 55 58 + NlaIII CATG 58 54 . . 55 58 + CviAII CATG 55 57 . . 70 75 + Cac8I GCNNGC 72 72 . . 73 76 + CviJI RGCY 74 74 . . 76 79 + FatI CATG 75 79 . . 76 79 + NlaIII CATG 79 75 . . 76 79 + CviAII CATG 76 78 . . 77 81 + TspDTI ATGAA 92 90 . . 102 113 + CspCI CAANNNNNGTGG 90 88 125 123 105 108 + TspEI AATT 104 108 . . 113 117 + MaeIII GTNAC 112 117 . . 129 133 + Hpy99I CGWCG 133 128 . . 133 137 + MboII GAAGA 145 144 . . 141 147 + DraII RGGNCCY 142 145 . . 141 146 + NlaIV GGNNCC 143 143 . . 142 146 + BmgT120I GGNCC 143 144 . . 142 146 + AsuI* GGNCC 142 145 . . 143 146 + CviJI RGCY 144 144 . . 143 146 + HaeIII GGCC 144 144 . . 145 149 + Hin4II* CCTTC 155 154 . . 146 151 - Eco57MI CTGRAG 131 129 . . 146 151 - Eco57I CTGAAG 131 129 . . 153 158 + ClaI ATCGAT 154 156 . . 154 157 + TaqI TCGA 154 156 . . 159 162 + CviJI RGCY 160 160 . . 160 170 + MwoI GCNNNNNNNGC 166 163 . . 156 160 - BccI CCATC 150 151 . . 206 209 + SetI ASST 209 205 . . 209 212 + MseI TTAA 209 211 . . 221 224 + DpnI GATC 222 222 . . 221 224 + BstKTI GATC 223 221 . . 221 224 + MboI GATC 220 224 . . 228 232 + BmgT120I GGNCC 229 230 . . 228 232 + AsuI* GGNCC 228 231 . . 229 232 + CviJI RGCY 230 230 . . 229 232 + HaeIII GGCC 230 230 . . 231 235 + Hin4II* CCTTC 241 240 . . 234 239 + Hpy178III* TCNNGA 235 237 . . 239 243 + TspDTI ATGAA 254 252 . . 263 266 + TspEI AATT 262 266 . . 268 271 + MaeI CTAG 268 270 . . 274 277 + SetI ASST 277 273 . . 272 276 - Hin4II* CCTTC 266 265 . . 277 281 + Hpy188I TCNGA 279 278 . . 302 306 + TspDTI ATGAA 317 315 . . 305 310 + SspI AATATT 307 307 . . 313 323 + Hin4I GAYNNNNNVTC 304 299 336 331 313 323 - Hin4I GAYNNNNNVTC 336 331 304 299 328 331 + TspEI AATT 327 331 . . 331 335 - MboII GAAGA 323 322 . . 336 346 + FalI AAGNNNNNCTT 327 322 359 354 340 343 + TspEI AATT 339 343 . . 346 350 + Hpy188I TCNGA 348 347 . . 336 346 - FalI AAGNNNNNCTT 359 354 327 322 343 347 - MboII GAAGA 335 334 . . 344 349 - Eco57MI CTGRAG 329 327 . . 344 349 - Eco57I CTGAAG 329 327 . . 363 374 + BdaI TGANNNNNNTCA 352 350 386 384 365 369 + BceAI ACGGC 381 383 . . 367 370 + CviJI RGCY 368 368 . . 372 377 + Hpy178III* TCNNGA 373 375 . . 363 374 - BdaI TGANNNNNNTCA 386 384 352 350 379 390 + TstI CACNNNNNNTCC 370 365 402 397 380 390 + BsaXI ACNNNNNCTCC 370 367 400 397 385 388 + SetI ASST 388 384 . . 386 389 + MnlI CCTC 396 395 . . 388 392 - TspGWI ACGGA 378 376 . . 400 403 + AciI CCGC 400 402 . . 401 408 + SgrAI CRCCGGYG 402 406 . . 401 408 + Sse232I* CGCCGGCG 402 406 . . 402 407 + NaeI GCCGGC 404 404 . . 402 407 + MroNI GCCGGC 402 406 . . 402 407 + Cac8I GCNNGC 404 404 . . 402 407 + Cfr10I RCCGGY 402 406 . . 403 406 + HpaII CCGG 403 405 . . 405 410 + EciI GGCGGA 421 419 . . 406 409 - AciI CCGC 406 408 . . 418 421 + SetI ASST 421 417 . . 418 421 + CviJI RGCY 419 419 . . 418 421 + AluI AGCT 419 419 . . 424 427 + MseI TTAA 424 426 . . 426 429 + TspEI AATT 425 429 . . 446 449 + TaiI ACGT 449 445 . . 446 449 + SetI ASST 449 445 . . 446 449 + MaeII ACGT 446 448 . . 458 461 + CviJI RGCY 459 459 . . 468 473 + BspLU11I* ACATGT 468 472 . . 468 473 + NspI RCATGY 472 468 . . 468 473 + AflIII ACRYGT 468 472 . . 469 472 + FatI CATG 468 472 . . 469 472 + NlaIII CATG 472 468 . . 469 472 + CviAII CATG 469 471 . . 472 477 + Hpy166II GTNNAC 474 474 . . 472 477 + HindII GTYRAC 474 474 . . 475 485 + Hin4I GAYNNNNNVTC 466 461 498 493 475 485 + Eam1105I GACNNNNNGTC 480 479 . . 483 487 + Tsp45I GTSAC 482 487 . . 483 487 + MaeIII GTNAC 482 487 . . 485 494 + MslI CAYNNNNRTG 489 489 . . 475 485 - Hin4I GAYNNNNNVTC 498 493 466 461 487 490 + FatI CATG 486 490 . . 487 490 + NlaIII CATG 490 486 . . 487 490 + CviAII CATG 487 489 . . 488 492 + TspDTI ATGAA 503 501 . . 499 503 + TfiI GAWTC 499 502 . . 499 503 + HinfI GANTC 499 502 . . 502 506 + Hpy188I TCNGA 504 503 . . 506 511 + ApoI RAATTY 506 510 . . 507 510 + TspEI AATT 506 510 . . 514 517 + TspEI AATT 513 517 . . 531 535 - FokI GGATG 517 521 . . 531 535 - BseGI GGATG 530 528 . . 553 562 + BsaBI GATNNNNATC 557 557 . . 559 562 + DpnI GATC 560 560 . . 559 562 + BstKTI GATC 561 559 . . 559 562 + MboI GATC 558 562 . . 562 565 + MaeI CTAG 562 564 . . 571 575 + BlsI GCNGC 573 572 . . 571 575 + BisI GCNGC 572 573 . . 571 575 + TseI GCWGC 571 574 . . 575 578 + MaeI CTAG 575 577 . . 571 575 - BbvI GCAGC 558 562 . . 577 580 + SetI ASST 580 576 . . 590 593 + MnlI CCTC 600 599 . . 593 596 + FatI CATG 592 596 . . 593 596 + NlaIII CATG 596 592 . . 593 596 + CviAII CATG 593 595 . . 602 605 + FatI CATG 601 605 . . 602 605 + NlaIII CATG 605 601 . . 602 605 + CviAII CATG 602 604 . . 631 635 + Tsp4CI* ACNGT 633 632 . . 632 638 + TspRI CASTGNN 638 629 . . 636 640 + Tsp45I GTSAC 635 640 . . 636 640 + MaeIII GTNAC 635 640 . . 639 642 + SetI ASST 642 638 . . 641 646 + Eco57MI CTGRAG 662 660 . . 637 641 - HphI GGTGA 629 628 . . 641 646 + Eco57I CTGAAG 662 660 . . 650 653 + MseI TTAA 650 652 . . 647 652 - TsoI TARCCA 637 635 . . 652 655 + TspEI AATT 651 655 . . 664 668 + TfiI GAWTC 664 667 . . 664 668 + HinfI GANTC 664 667 . . 667 671 + Hpy188I TCNGA 669 668 . . 673 677 + Tsp45I GTSAC 672 677 . . 673 677 + MaeIII GTNAC 672 677 . . 680 683 + CviJI RGCY 681 681 . . 679 684 - TsoI TARCCA 669 667 . . 688 691 + CviJI RGCY 689 689 . . 688 691 + HaeIII GGCC 689 689 . . 688 692 + BmgT120I GGNCC 689 690 . . 688 692 + AsuI* GGNCC 688 691 . . 703 707 + TfiI GAWTC 703 706 . . 703 707 + HinfI GANTC 703 706 . . 706 710 + Hpy188I TCNGA 708 707 . . 707 712 + Eco57MI CTGRAG 728 726 . . 707 712 + Eco57I CTGAAG 728 726 . . 711 714 + SetI ASST 714 710 . . 709 713 - Hin4II* CCTTC 703 702 . . 727 731 + MboII GAAGA 739 738 . . 730 734 + MboII GAAGA 742 741 . . 736 739 + FatI CATG 735 739 . . 736 739 + NlaIII CATG 739 735 . . 736 739 + CviAII CATG 736 738 . . 739 742 + DpnI GATC 740 740 . . 739 742 + BstKTI GATC 741 739 . . 739 742 + MboI GATC 738 742 . . 741 746 + XbaI TCTAGA 741 745 . . 741 746 + Hpy178III* TCNNGA 742 744 . . 742 745 + MaeI CTAG 742 744 . . 749 752 - MnlI CCTC 742 741 . . 760 764 + Hpy188I TCNGA 762 761 . . 762 766 + FokI GGATG 775 779 . . 762 766 + BseGI GGATG 768 766 . . 767 771 + TspDTI ATGAA 782 780 . . 771 776 + ApoI RAATTY 771 775 . . 772 775 + TspEI AATT 771 775 . . 782 787 + ApoI RAATTY 782 786 . . 783 786 + TspEI AATT 782 786 . . 801 806 + ApoI RAATTY 801 805 . . 802 805 + TspEI AATT 801 805 . . 811 814 + TaqI TCGA 811 813 . . 812 817 + McrI* CGRYCG 815 813 . . 812 817 + PvuI CGATCG 815 813 . . 813 816 + DpnI GATC 814 814 . . 813 816 + BstKTI GATC 815 813 . . 813 816 + MboI GATC 812 816 . . 817 821 + BlsI GCNGC 819 818 . . 817 821 + BisI GCNGC 818 819 . . 817 821 + BbvI GCAGC 829 833 . . 817 821 + TseI GCWGC 817 820 . . 818 823 + PvuII CAGCTG 820 820 . . 818 823 + NspBII* CMGCKG 820 820 . . 819 822 + SetI ASST 822 818 . . 819 822 + CviJI RGCY 820 820 . . 819 822 + AluI AGCT 820 820 . . 820 824 + BlsI GCNGC 822 821 . . 820 824 + BisI GCNGC 821 822 . . 820 824 + TseI GCWGC 820 823 . . 820 824 - BbvI GCAGC 807 811 . . 825 829 + Hpy188I TCNGA 827 826 . . 834 837 + TspEI AATT 833 837 . . 840 844 + MboII GAAGA 852 851 . . 843 847 + MboII GAAGA 855 854 . . 843 848 - Ksp632I* CTCTTC 838 841 . . 850 854 + Tsp4CI* ACNGT 852 851 . . 853 857 + Tsp45I GTSAC 852 857 . . 853 857 + MaeIII GTNAC 852 857 . . 861 864 + TspEI AATT 860 864 . . 868 871 - MnlI CCTC 861 860 . . 895 898 - MnlI CCTC 888 887 . . 909 912 + FatI CATG 908 912 . . 909 912 + NlaIII CATG 912 908 . . 909 912 + CviAII CATG 909 911 . . 919 923 + TspDTI ATGAA 934 932 . . 924 929 + NspI RCATGY 928 924 . . 925 928 + FatI CATG 924 928 . . 925 928 + NlaIII CATG 928 924 . . 925 928 + CviAII CATG 925 927 . . 942 947 + XhoII RGATCY 942 946 . . 942 947 + NlaIV GGNNCC 944 944 . . 942 947 + BamHI GGATCC 942 946 . . 942 946 + BinI* GGATC 950 951 . . 943 946 + DpnI GATC 944 944 . . 943 946 + BstKTI GATC 945 943 . . 943 946 + MboI GATC 942 946 . . 943 947 - BinI* GGATC 937 938 . . 962 965 + TaiI ACGT 965 961 . . 962 965 + SetI ASST 965 961 . . 962 965 + MaeII ACGT 962 964 . . 967 970 + FatI CATG 966 970 . . 967 970 + NlaIII CATG 970 966 . . 967 970 + CviAII CATG 967 969 . . 972 983 + BcgI CGANNNNNNTGC 961 959 995 993 977 980 + TaqI TCGA 977 979 . . 982 992 + MwoI GCNNNNNNNGC 988 985 . . 979 983 - SfaNI GCATC 969 973 . . 986 991 + CfrI YGGCCR 986 990 . . 987 990 + CviJI RGCY 988 988 . . 987 990 + HaeIII GGCC 988 988 . . 988 992 + TauI GCSGC 991 988 . . 988 992 + BlsI GCNGC 990 989 . . 988 992 + BisI GCNGC 989 990 . . 989 992 + AciI CCGC 989 991 . . 989 994 + BsrBI CCGCTC 991 991 . . 992 997 + SmlI CTYRAG 992 996 . . 997 1002 + Hpy166II GTNNAC 999 999 . . 997 1002 + HpaI GTTAAC 999 999 . . 997 1002 + HindII GTYRAC 999 999 . . 992 997 - Bce83I* CTTGAG 977 975 . . 998 1001 + MseI TTAA 998 1000 . . 1007 1012 + TatI WGTACW 1007 1011 . . 1007 1012 + ScaI AGTACT 1009 1009 . . 1008 1011 + Csp6I GTAC 1008 1010 . . 1008 1011 + RsaI GTAC 1009 1009 . . 1005 1009 - BsrI ACTGG 1005 1003 . . 1004 1009 - BfiI ACTGGG 999 998 . . 1027 1031 + TfiI GAWTC 1027 1030 . . 1027 1031 + HinfI GANTC 1027 1030 . . 1030 1033 + TaqI TCGA 1030 1032 . . 1034 1038 + Tsp4CI* ACNGT 1036 1035 . . 1050 1054 + MboII GAAGA 1062 1061 . . 1053 1057 + PleI GAGTC 1061 1062 . . 1053 1057 + MlyI GAGTC 1062 1062 . . 1053 1057 + HinfI GANTC 1053 1056 . . 1055 1060 + Hpy166II GTNNAC 1057 1057 . . 1055 1060 + HindII GTYRAC 1057 1057 . . 1050 1055 - Ksp632I* CTCTTC 1045 1048 . . 1063 1067 + Hpy188I TCNGA 1065 1064 . . 1078 1081 + TspEI AATT 1077 1081 . . 1092 1096 + TfiI GAWTC 1092 1095 . . 1092 1096 + HinfI GANTC 1092 1095 . . 1097 1102 + SfeI* CTRYAG 1097 1101 . . 1107 1112 + ApoI RAATTY 1107 1111 . . 1108 1111 + TspEI AATT 1107 1111 . . 1123 1127 + HphI GGTGA 1135 1134 . . 1124 1128 + Tsp45I GTSAC 1123 1128 . . 1124 1128 + MaeIII GTNAC 1123 1128 . . 1121 1124 - AciI CCGC 1121 1123 . . 1135 1139 + MboII GAAGA 1147 1146 . . 1138 1142 + MboII GAAGA 1150 1149 . . 1138 1143 + BbvII* GAAGAC 1145 1149 . . 1142 1145 + SetI ASST 1145 1141 . . 1142 1147 + BspMI ACCTGC 1151 1155 . . 1145 1148 + CviRI* TGCA 1146 1146 . . 1149 1154 + AhaIII* TTTAAA 1151 1151 . . 1150 1153 + MseI TTAA 1150 1152 . . 1158 1163 + HaeII RGCGCY 1162 1158 . . 1158 1163 + Eco47III AGCGCT 1160 1160 . . 1159 1162 + Hin6I GCGC 1159 1161 . . 1159 1162 + GlaI GCGC 1160 1160 . . 1159 1162 + HhaI GCGC 1161 1159 . . 1165 1168 + FatI CATG 1164 1168 . . 1165 1168 + NlaIII CATG 1168 1164 . . 1165 1168 + CviAII CATG 1165 1167 . . 1163 1167 - TspDTI ATGAA 1153 1151 . . 1180 1183 + SetI ASST 1183 1179 . . 1182 1187 + Hpy166II GTNNAC 1184 1184 . . 1182 1187 + HindII GTYRAC 1184 1184 . . 1186 1190 + BceAI ACGGC 1202 1204 . . 1210 1213 + MaeI CTAG 1210 1212 . . 1220 1223 + Csp6I GTAC 1220 1222 . . 1220 1223 + RsaI GTAC 1221 1221 . . 1228 1231 + DpnI GATC 1229 1229 . . 1228 1231 + BstKTI GATC 1230 1228 . . 1228 1231 + MboI GATC 1227 1231 . . 1234 1244 + FalI AAGNNNNNCTT 1225 1220 1257 1252 1236 1239 + CviJI RGCY 1237 1237 . . 1234 1244 - FalI AAGNNNNNCTT 1257 1252 1225 1220 1245 1249 + BinI* GGATC 1253 1254 . . 1246 1249 + DpnI GATC 1247 1247 . . 1246 1249 + BstKTI GATC 1248 1246 . . 1246 1249 + MboI GATC 1245 1249 . . 1253 1256 + SetI ASST 1256 1252 . . 1257 1262 + ApoI RAATTY 1257 1261 . . 1258 1261 + TspEI AATT 1257 1261 . . 1263 1268 + Hpy178III* TCNNGA 1264 1266 . . 1276 1279 + SetI ASST 1279 1275 . . 1277 1282 + NlaIV GGNNCC 1279 1279 . . 1283 1286 + MnlI CCTC 1293 1292 . . 1286 1289 + MnlI CCTC 1296 1295 . . 1284 1289 - BseRI GAGGAG 1275 1273 . . 1298 1301 + MnlI CCTC 1308 1307 . . 1294 1298 - MboII GAAGA 1286 1285 . . 1313 1317 + TspDTI ATGAA 1328 1326 . . 1321 1324 + TspEI AATT 1320 1324 . . 1325 1330 + DsaI* CCRYGG 1325 1329 . . 1325 1330 + StyI CCWWGG 1325 1329 . . 1325 1330 + NcoI CCATGG 1325 1329 . . 1325 1330 + SecI* CCNNGG 1325 1329 . . 1326 1329 + FatI CATG 1325 1329 . . 1326 1329 + NlaIII CATG 1329 1325 . . 1326 1329 + CviAII CATG 1326 1328 . . 1331 1340 + BsaBI GATNNNNATC 1335 1335 . . 1341 1346 + SspI AATATT 1343 1343 . . 1345 1348 + MseI TTAA 1345 1347 . . 1348 1351 + SetI ASST 1351 1347 . . 1349 1352 + MnlI CCTC 1359 1358 . . 1377 1380 + SetI ASST 1380 1376 . . 1399 1408 + MslI CAYNNNNRTG 1403 1403 . . 1403 1406 + MseI TTAA 1403 1405 . . 1436 1441 + EcoRV GATATC 1438 1438 . . 1443 1446 + SetI ASST 1446 1442 . . 1443 1446 + CviJI RGCY 1444 1444 . . 1443 1446 + AluI AGCT 1444 1444 . . 1453 1457 + Hpy188I TCNGA 1455 1454 . . 1458 1462 + FokI GGATG 1471 1475 . . 1458 1462 + BseGI GGATG 1464 1462 . . 1456 1459 - MnlI CCTC 1449 1448 . . 1463 1467 + TspDTI ATGAA 1478 1476 . . 1469 1474 + BtgZI GCGATG 1484 1488 . . 1475 1479 + TspDTI ATGAA 1490 1488 . . 1499 1502 + MseI TTAA 1499 1501 . . 1513 1518 + AclI AACGTT 1514 1516 . . 1514 1517 + TaiI ACGT 1517 1513 . . 1514 1517 + SetI ASST 1517 1513 . . 1514 1517 + MaeII ACGT 1514 1516 . . 1516 1520 + MaeIII GTNAC 1515 1520 . . 1539 1543 + HinfI GANTC 1539 1542 . . 1539 1543 - PleI GAGTC 1533 1534 . . 1539 1543 - MlyI GAGTC 1533 1533 . . 1548 1553 + Cac8I GCNNGC 1550 1550 . . 1548 1553 + BmtI GCTAGC 1552 1548 . . 1548 1553 + NheI GCTAGC 1548 1552 . . 1544 1549 - BsrDI GCAATG 1543 1541 . . 1549 1552 + MaeI CTAG 1549 1551 . . 1550 1555 + TsoI TARCCA 1566 1564 . . 1551 1554 + CviJI RGCY 1552 1552 . . 1583 1586 + AciI CCGC 1583 1585 . . 1610 1613 + MaeI CTAG 1610 1612 . . 1614 1618 + Hpy99I CGWCG 1618 1613 . . 1618 1628 + Hin4I GAYNNNNNVTC 1609 1604 1641 1636 1621 1631 + Hin4I GAYNNNNNVTC 1612 1607 1644 1639 1628 1631 + MnlI CCTC 1638 1637 . . 1618 1628 - Hin4I GAYNNNNNVTC 1641 1636 1609 1604 1625 1629 - FokI GGATG 1611 1615 . . 1625 1629 - BseGI GGATG 1624 1622 . . 1642 1646 - MboII GAAGA 1634 1633 . . 1644 1648 - TspDTI ATGAA 1634 1632 . . 1651 1660 + MslI CAYNNNNRTG 1655 1655 . . 1654 1658 + Hpy188I TCNGA 1656 1655 . . 1661 1665 + TspDTI ATGAA 1676 1674 . . 1663 1667 + MboII GAAGA 1675 1674 . . 1670 1674 + BceAI ACGGC 1686 1688 . . 1673 1677 + TauI GCSGC 1676 1673 . . 1673 1677 + BlsI GCNGC 1675 1674 . . 1673 1677 + BisI GCNGC 1674 1675 . . 1675 1678 + CviJI RGCY 1676 1676 . . 1675 1678 + HaeIII GGCC 1676 1676 . . 1673 1676 - AciI CCGC 1673 1675 . . 1689 1692 + CviRI* TGCA 1690 1690 . . 1699 1705 + PfoI TCCNGGA 1699 1704 . . 1700 1704 + BssKI CCNGG 1699 1704 . . 1700 1704 + BseBI CCWGG 1701 1702 . . 1700 1704 + ScrFI CCNGG 1701 1702 . . 1700 1704 + EcoRII CCWGG 1699 1704 . . 1705 1708 + SetI ASST 1708 1704 . . 1704 1707 - MnlI CCTC 1697 1696 . . 1708 1712 + Hpy188I TCNGA 1710 1709 . . 1711 1715 + HinfI GANTC 1711 1714 . . 1714 1719 + Hpy178III* TCNNGA 1715 1717 . . 1711 1715 - PleI GAGTC 1705 1706 . . 1711 1715 - MlyI GAGTC 1705 1705 . . 1715 1720 + Eco57MI CTGRAG 1736 1734 . . 1715 1720 + GsuI CTGGAG 1736 1734 . . 1721 1725 - TspDTI ATGAA 1711 1709 . . 1726 1730 - HphI GGTGA 1718 1717 . . 1738 1748 + Hin4I GAYNNNNNVTC 1729 1724 1761 1756 1741 1744 + TspEI AATT 1740 1744 . . 1745 1748 + MnlI CCTC 1755 1754 . . 1752 1755 + CviJI RGCY 1753 1753 . . 1755 1759 - TspDTI ATGAA 1745 1743 . . 1768 1773 + Hpy178III* TCNNGA 1769 1771 . . 1772 1777 + SacI GAGCTC 1776 1772 . . 1772 1777 + Ecl136II GAGCTC 1774 1774 . . 1772 1777 + SduI GDGCHC 1776 1772 . . 1772 1777 + HgiJII* GRGCYC 1776 1772 . . 1772 1777 + HgiAI* GWGCWC 1776 1772 . . 1773 1776 + SetI ASST 1776 1772 . . 1773 1776 + CviJI RGCY 1774 1774 . . 1773 1776 + AluI AGCT 1774 1774 . . 1777 1780 + FatI CATG 1776 1780 . . 1777 1780 + NlaIII CATG 1780 1776 . . 1777 1780 + CviAII CATG 1777 1779 . . 1780 1783 + Csp6I GTAC 1780 1782 . . 1780 1783 + RsaI GTAC 1781 1781 . . 1782 1785 + TaiI ACGT 1785 1781 . . 1782 1785 + SetI ASST 1785 1781 . . 1782 1785 + MaeII ACGT 1782 1784 . . 1784 1788 - BslFI GGGAC 1769 1773 . . 1803 1808 + Hpy178III* TCNNGA 1804 1806 . . 1819 1822 + TspEI AATT 1818 1822 . . 1825 1829 + BinI* GGATC 1833 1834 . . 1826 1829 + DpnI GATC 1827 1827 . . 1826 1829 + BstKTI GATC 1828 1826 . . 1826 1829 + MboI GATC 1825 1829 . . 1828 1832 + Hpy188I TCNGA 1830 1829 . . 1831 1841 + Hin4I GAYNNNNNVTC 1822 1817 1854 1849 1834 1838 + MboII GAAGA 1846 1845 . . 1837 1841 + TfiI GAWTC 1837 1840 . . 1837 1841 + HinfI GANTC 1837 1840 . . 1840 1844 + Hpy188I TCNGA 1842 1841 . . 1831 1841 - Hin4I GAYNNNNNVTC 1854 1849 1822 1817 1852 1857 + ApoI RAATTY 1852 1856 . . 1853 1856 + TspEI AATT 1852 1856 . . 1856 1859 + MseI TTAA 1856 1858 . . 1878 1882 + Tsp45I GTSAC 1877 1882 . . 1878 1882 + MaeIII GTNAC 1877 1882 . . 1886 1889 + TaiI ACGT 1889 1885 . . 1886 1889 + SetI ASST 1889 1885 . . 1886 1889 + MaeII ACGT 1886 1888 . . 1894 1897 + SetI ASST 1897 1893 . . 1895 1898 + MnlI CCTC 1905 1904 . . 1897 1902 + Hpy178III* TCNNGA 1898 1900 . . 1896 1901 - Eco57MI CTGRAG 1881 1879 . . 1896 1901 - GsuI CTGGAG 1881 1879 . . 1903 1906 + CviJI RGCY 1904 1904 . . 1901 1904 - MnlI CCTC 1894 1893 . . 1921 1931 + Hin4I GAYNNNNNVTC 1912 1907 1944 1939 1923 1928 + PsiI TTATAA 1925 1925 . . 1933 1938 + Hpy166II GTNNAC 1935 1935 . . 1933 1938 + AccI GTMKAC 1934 1936 . . 1930 1935 - TaqII GACCGA 1920 1918 . . 1938 1944 + TspRI CASTGNN 1944 1935 . . 1939 1943 + BsrI ACTGG 1944 1942 . . 1942 1946 + HphI GGTGA 1954 1953 . . 1952 1956 - BsrI ACTGG 1952 1950 . . 1963 1967 - BsrI ACTGG 1963 1961 . . 1969 1973 + TfiI GAWTC 1969 1972 . . 1969 1973 + HinfI GANTC 1969 1972 . . 1973 1976 + FatI CATG 1972 1976 . . 1973 1976 + NlaIII CATG 1976 1972 . . 1973 1976 + CviAII CATG 1973 1975 . . 1971 1975 - TspDTI ATGAA 1961 1959 . . 1976 1980 + Tsp45I GTSAC 1975 1980 . . 1976 1980 + MaeIII GTNAC 1975 1980 . . 1992 1997 + Hpy178III* TCNNGA 1993 1995 . . 1994 1999 + Eco57MI CTGRAG 2015 2013 . . 1994 1999 + Eco57I CTGAAG 2015 2013 . . 1996 2000 - Hin4II* CCTTC 1990 1989 . . 2016 2019 + CviJI RGCY 2017 2017 . . 2022 2025 + CviRI* TGCA 2023 2023 . . 2019 2024 - BsrDI GCAATG 2018 2016 . . 2029 2039 + Hin4I GAYNNNNNVTC 2020 2015 2052 2047 2033 2038 + EcoT22I ATGCAT 2037 2033 . . 2034 2037 + CviRI* TGCA 2035 2035 . . 2035 2039 + SfaNI GCATC 2044 2048 . . 2029 2039 - Hin4I GAYNNNNNVTC 2052 2047 2020 2015 2045 2049 + TspDTI ATGAA 2060 2058 . . 2047 2052 + ApoI RAATTY 2047 2051 . . 2048 2051 + TspEI AATT 2047 2051 . . 2051 2054 + MseI TTAA 2051 2053 . . 2059 2062 - AciI CCGC 2059 2061 . . 2065 2069 + TfiI GAWTC 2065 2068 . . 2065 2069 + HinfI GANTC 2065 2068 . . 2069 2074 + StyI CCWWGG 2069 2073 . . 2069 2074 + SecI* CCNNGG 2069 2073 . . 2079 2084 + BspLU11I* ACATGT 2079 2083 . . 2079 2084 + NspI RCATGY 2083 2079 . . 2079 2084 + AflIII ACRYGT 2079 2083 . . 2080 2083 + FatI CATG 2079 2083 . . 2080 2083 + NlaIII CATG 2083 2079 . . 2080 2083 + CviAII CATG 2080 2082 . . 2099 2104 + HindIII AAGCTT 2099 2103 . . 2100 2103 + SetI ASST 2103 2099 . . 2100 2103 + CviJI RGCY 2101 2101 . . 2100 2103 + AluI AGCT 2101 2101 . . 2102 2107 - Eco57MI CTGRAG 2087 2085 . . 2102 2107 - Eco57I CTGAAG 2087 2085 . . 2119 2124 + AflIII ACRYGT 2119 2123 . . 2120 2125 + BtrI CACGTC 2122 2122 . . 2121 2124 + TaiI ACGT 2124 2120 . . 2121 2124 + SetI ASST 2124 2120 . . 2121 2124 + MaeII ACGT 2121 2123 . . 2123 2128 + Hpy166II GTNNAC 2125 2125 . . 2123 2128 + HindII GTYRAC 2125 2125 . . 2128 2131 + FatI CATG 2127 2131 . . 2128 2131 + NlaIII CATG 2131 2127 . . 2128 2131 + CviAII CATG 2128 2130 . . 2131 2135 + BinI* GGATC 2139 2140 . . 2132 2135 + DpnI GATC 2133 2133 . . 2132 2135 + BstKTI GATC 2134 2132 . . 2132 2135 + MboI GATC 2131 2135 . . 2137 2142 + NdeI CATATG 2138 2140 . . 2154 2158 + Hpy99I CGWCG 2158 2153 . . 2161 2164 + TspEI AATT 2160 2164 . . 2167 2170 + SetI ASST 2170 2166 . . 2167 2170 + CviJI RGCY 2168 2168 . . 2167 2170 + AluI AGCT 2168 2168 . . 2168 2174 + SapI GCTCTTC 2175 2178 . . 2169 2174 + Ksp632I* CTCTTC 2175 2178 . . 2170 2174 - MboII GAAGA 2162 2161 . . 2172 2176 - TspDTI ATGAA 2162 2160 . . 2176 2180 + Hpy188I TCNGA 2178 2177 . . 2192 2195 + TspEI AATT 2191 2195 . . 2200 2204 + MboII GAAGA 2212 2211 . . 2200 2205 + BbvII* GAAGAC 2207 2211 . . 2203 2207 + HinfI GANTC 2203 2206 . . 2203 2207 - PleI GAGTC 2197 2198 . . 2203 2207 - MlyI GAGTC 2197 2197 . . 2223 2227 + Hpy99I CGWCG 2227 2222 . . 2226 2230 + Hpy99I CGWCG 2230 2225 . . 2230 2234 + MboII GAAGA 2242 2241 . . 2230 2235 - Ksp632I* CTCTTC 2225 2228 . . 2233 2236 - MnlI CCTC 2226 2225 . . 2236 2240 + MboII GAAGA 2248 2247 . . 2239 2243 + MboII GAAGA 2251 2250 . . 2260 2265 + Hpy166II GTNNAC 2262 2262 . . 2260 2265 + AccI GTMKAC 2261 2263 . . 2268 2273 + TsoI TARCCA 2284 2282 . . 2276 2280 + TspDTI ATGAA 2291 2289 . . 2278 2282 - Hin4II* CCTTC 2272 2271 . . 2288 2294 + TspRI CASTGNN 2294 2285 . . 2290 2294 + DdeI CTNAG 2290 2293 . . 2292 2297 + BseRI GAGGAG 2307 2305 . . 2290 2294 - BspCNI CTCAG 2282 2280 . . 2290 2294 - BseMII CTCAG 2281 2279 . . 2292 2295 - MnlI CCTC 2285 2284 . . 2304 2308 + Tsp45I GTSAC 2303 2308 . . 2304 2308 + MaeIII GTNAC 2303 2308 . . 2317 2322 + Hpy178III* TCNNGA 2318 2320 . . 2320 2325 + XhoII RGATCY 2320 2324 . . 2320 2324 + BinI* GGATC 2328 2329 . . 2321 2324 + DpnI GATC 2322 2322 . . 2321 2324 + BstKTI GATC 2323 2321 . . 2321 2324 + MboI GATC 2320 2324 . . 2329 2332 + TspEI AATT 2328 2332 . . 2337 2340 + Csp6I GTAC 2337 2339 . . 2337 2340 + RsaI GTAC 2338 2338 . . 2341 2344 + DpnI GATC 2342 2342 . . 2341 2344 + BstKTI GATC 2343 2341 . . 2341 2344 + MboI GATC 2340 2344 . . 2344 2347 + MaeI CTAG 2344 2346 . . 2353 2357 + BccI CCATC 2361 2362 . . 2361 2366 + Ksp632I* CTCTTC 2367 2370 . . 2362 2366 - MboII GAAGA 2354 2353 . . 2374 2377 + MnlI CCTC 2384 2383 . . 2372 2377 - BseRI GAGGAG 2363 2361 . . 2395 2398 + TspEI AATT 2394 2398 . . 2419 2424 + Hpy166II GTNNAC 2421 2421 . . 2419 2424 + AccI GTMKAC 2420 2422 . . 2430 2433 + CviRI* TGCA 2431 2431 . . 2431 2441 + MwoI GCNNNNNNNGC 2437 2434 . . 2428 2432 - SfaNI GCATC 2418 2422 . . 2436 2440 + BsrI ACTGG 2441 2439 . . 2439 2442 + CviJI RGCY 2440 2440 . . 2447 2450 + CviJI RGCY 2448 2448 . . 2447 2450 + HaeIII GGCC 2448 2448 . . 2451 2456 + BciVI GTATCC 2462 2461 . . 2448 2452 - BceAI ACGGC 2433 2435 . . 2454 2458 - TspGWI ACGGA 2444 2442 . . 2463 2466 + Hin6I GCGC 2463 2465 . . 2463 2466 + GlaI GCGC 2464 2464 . . 2463 2466 + HhaI GCGC 2465 2463 . . 2467 2470 + TspEI AATT 2466 2470 . . 2471 2474 + MnlI CCTC 2481 2480 . . 2471 2481 + BsiYI* CCNNNNNNNGG 2477 2474 . . 2472 2477 + Ksp632I* CTCTTC 2478 2481 . . 2473 2477 - MboII GAAGA 2465 2464 . . 2474 2479 - Eco57MI CTGRAG 2459 2457 . . 2474 2479 - Eco57I CTGAAG 2459 2457 . . 2485 2489 - TspDTI ATGAA 2475 2473 . . 2494 2497 + TspEI AATT 2493 2497 . . 2497 2501 + Hpy188I TCNGA 2499 2498 . . 2506 2510 + MboII GAAGA 2518 2517 . . 2509 2513 + MboII GAAGA 2521 2520 . . 2509 2514 - Ksp632I* CTCTTC 2504 2507 . . 2519 2523 + BlsI GCNGC 2521 2520 . . 2519 2523 + BisI GCNGC 2520 2521 . . 2519 2523 + BbvI GCAGC 2531 2535 . . 2519 2523 + TseI GCWGC 2519 2522 . . 2524 2529 + BseRI GAGGAG 2539 2537 . . 2524 2527 - MnlI CCTC 2517 2516 . . 2527 2530 - MnlI CCTC 2520 2519 . . 2545 2549 + FauI CCCGC 2553 2555 . . 2546 2549 + AciI CCGC 2546 2548 . . 2552 2556 + Tsp4CI* ACNGT 2554 2553 . . 2557 2562 + PvuII CAGCTG 2559 2559 . . 2557 2562 + NspBII* CMGCKG 2559 2559 . . 2558 2561 + SetI ASST 2561 2557 . . 2558 2561 + CviJI RGCY 2559 2559 . . 2558 2561 + AluI AGCT 2559 2559 . . 2564 2567 + MseI TTAA 2564 2566 . . 2561 2566 - TsoI TARCCA 2551 2549 . . 2568 2571 + TaqI TCGA 2568 2570 . . 2585 2590 + BtsI GCAGTG 2592 2590 . . 2586 2592 + TspRI CASTGNN 2592 2583 . . 2590 2594 + Tsp45I GTSAC 2589 2594 . . 2590 2594 + MaeIII GTNAC 2589 2594 . . 2612 2616 + DdeI CTNAG 2612 2615 . . 2612 2616 + BspCNI CTCAG 2625 2623 . . 2612 2616 + BseMII CTCAG 2626 2624 . . 2613 2617 + Hpy188I TCNGA 2615 2614 . . 2616 2620 + MboII GAAGA 2628 2627 . . 2625 2628 + CviJI RGCY 2626 2626 . . 2628 2632 + Hin4II* CCTTC 2638 2637 . . 2637 2641 + BlsI GCNGC 2639 2638 . . 2637 2641 + BisI GCNGC 2638 2639 . . 2637 2641 + BbvI GCAGC 2649 2653 . . 2637 2641 + TseI GCWGC 2637 2640 . . 2639 2642 + CviJI RGCY 2640 2640 . . 2650 2654 + Hpy188I TCNGA 2652 2651 . . 2647 2651 - HgaI GACGC 2636 2641 . . 2657 2662 + Hpy166II GTNNAC 2659 2659 . . 2664 2669 + HindIII AAGCTT 2664 2668 . . 2665 2668 + SetI ASST 2668 2664 . . 2665 2668 + CviJI RGCY 2666 2666 . . 2665 2668 + AluI AGCT 2666 2666 . . 2673 2676 + TspEI AATT 2672 2676 . . 2689 2693 + Hin4II* CCTTC 2699 2698 . . 2692 2696 - HphI GGTGA 2684 2683 . . 2697 2700 + CviJI RGCY 2698 2698 . . 2699 2709 + BsiYI* CCNNNNNNNGG 2705 2702 . . 2728 2731 - MnlI CCTC 2721 2720 . . 2744 2747 + MaeI CTAG 2744 2746 . . 2750 2754 + TspDTI ATGAA 2765 2763 . . 2752 2756 + TfiI GAWTC 2752 2755 . . 2752 2756 + HinfI GANTC 2752 2755 . . 2774 2778 + BssKI CCNGG 2773 2778 . . 2774 2777 + HpaII CCGG 2774 2776 . . 2774 2779 + SecI* CCNNGG 2774 2778 . . 2774 2778 + ScrFI CCNGG 2775 2776 . . 2774 2778 + CauII* CCSGG 2775 2776 . . 2787 2797 + Hin4I GAYNNNNNVTC 2778 2773 2810 2805 2790 2794 + BmgT120I GGNCC 2791 2792 . . 2790 2794 + AvaII GGWCC 2790 2793 . . 2790 2794 + AsuI* GGNCC 2790 2793 . . 2787 2791 - BccI CCATC 2781 2782 . . 2793 2797 + BccI CCATC 2801 2802 . . 2796 2801 + Hpy178III* TCNNGA 2797 2799 . . 2787 2797 - Hin4I GAYNNNNNVTC 2810 2805 2778 2773 2800 2803 + DpnI GATC 2801 2801 . . 2800 2803 + BstKTI GATC 2802 2800 . . 2800 2803 + MboI GATC 2799 2803 . . 2805 2809 + DdeI CTNAG 2805 2808 . . 2805 2809 + BspCNI CTCAG 2818 2816 . . 2805 2809 + BseMII CTCAG 2819 2817 . . 2806 2810 + Hpy188I TCNGA 2808 2807 . . 2858 2861 + MaeI CTAG 2858 2860 . . 2864 2867 + SetI ASST 2867 2863 . . 2864 2867 + CviJI RGCY 2865 2865 . . 2864 2867 + AluI AGCT 2865 2865 . . 2888 2893 + Cac8I GCNNGC 2890 2890 . . 2891 2894 + CviRI* TGCA 2892 2892 . . 2900 2903 + MaeI CTAG 2900 2902 . . 2911 2915 - HgaI GACGC 2900 2905 . . 2920 2924 + TspGWI ACGGA 2935 2933 . . 2923 2927 + TfiI GAWTC 2923 2926 . . 2923 2927 + HinfI GANTC 2923 2926 . . 2926 2929 + TaqI TCGA 2926 2928 . . 2933 2937 + TspDTI ATGAA 2948 2946 . . 2937 2942 + HindIII AAGCTT 2937 2941 . . 2938 2941 + SetI ASST 2941 2937 . . 2938 2941 + CviJI RGCY 2939 2939 . . 2938 2941 + AluI AGCT 2939 2939 . . 2944 2952 + DraIII CACNNNGTG 2949 2946 . . 2953 2958 + BsgI GTGCAG 2974 2972 . . 2954 2957 + CviRI* TGCA 2955 2955 . . 2958 2963 + BsmI GAATGC 2964 2962 . . 2961 2964 + CviRI* TGCA 2962 2962 . . 2967 2970 + SetI ASST 2970 2966 . . 2966 2969 - MnlI CCTC 2959 2958 . . 2974 2979 + Cac8I GCNNGC 2976 2976 . . 2978 2989 + AlfI GCANNNNNNTGC 2967 2965 3001 2999 2981 2986 + TatI WGTACW 2981 2985 . . 2981 2986 + ScaI AGTACT 2983 2983 . . 2982 2985 + Csp6I GTAC 2982 2984 . . 2982 2985 + RsaI GTAC 2983 2983 . . 2987 2990 + CviRI* TGCA 2988 2988 . . 2978 2989 - AlfI GCANNNNNNTGC 3001 2999 2967 2965 3005 3009 + Tsp45I GTSAC 3004 3009 . . 3005 3009 + MaeIII GTNAC 3004 3009 . . 3007 3012 + ZraI GACGTC 3009 3009 . . 2996 3007 - TstI CACNNNNNNTCC 3020 3015 2988 2983 3007 3012 + AcyI GRCGYC 3008 3010 . . 3007 3012 + AatII GACGTC 3011 3007 . . 3008 3011 + TaiI ACGT 3011 3007 . . 3008 3011 + SetI ASST 3011 3007 . . 3008 3011 + MaeII ACGT 3008 3010 . . 3014 3017 + CviJI RGCY 3015 3015 . . #--------------------------------------- # # # AatII: # [GACGTC] # 34 3011 # # AccI: # [GTMKAC] # 159 327 625 1934 # # AciI: # [CCGC] # 6 90 132 386 400 462 # 487 499 583 # # AclI: # [AACGTT] # 1514 1531 # # AcyI: # [GRCGYC] # 37 3008 # # AflIII: # [ACRYGT] # 40 468 926 1611 # # AhaIII*: # [TTTAAA] # 1151 1894 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 44 2967 # # AluI: # [AGCT] # 67 74 106 107 199 327 # 330 391 401 419 624 # # ApoI: # [RAATTY] # 11 19 150 195 265 306 # 506 595 998 # # AsuI*: # [GGNCC] # 86 142 255 460 2102 # # AvaII: # [GGWCC] # 255 2790 # # BamHI: # [GGATCC] # 942 2103 # # BbvI: # [GCAGC] # 22 118 249 396 558 1702 # # BbvII*: # [GAAGAC] # 838 1062 1145 # # BccI: # [CCATC] # 20 150 244 420 2211 # # Bce83I*: # [CTTGAG] # 977 2068 # # BceAI: # [ACGGC] # 381 484 612 747 821 # # BcgI: # [CGANNNNNNTGC] # 34 961 2050 # # BciVI: # [GTATCC] # 583 2462 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 352 2659 # # BfiI: # [ACTGGG] # 999 2046 # # BinI*: # [GGATC] # 13 189 303 306 580 717 # 937 # # BisI: # [GCNGC] # 3 118 168 246 407 572 # 685 846 # # BlsI: # [GCNGC] # 3 118 168 246 406 573 # 685 846 # # BmgT120I: # [GGNCC] # 86 143 254 460 2102 # # BmtI: # [GCTAGC] # 1493 1552 # # BsaBI: # [GATNNNNATC] # 557 778 1710 # # BsaXI: # [ACNNNNNCTCC] # 30 370 2645 # # BseBI: # [CCWGG] # 1344 1701 # # BseGI: # [GGATG] # 160 238 530 696 1421 # # BseMII: # [CTCAG] # 193 226 345 2281 # # BseRI: # [GAGGAG] # 56 176 506 1032 1275 # # BsgI: # [GTGCAG] # 71 2974 # # BsiYI*: # [CCNNNNNNNGG] # 228 340 2477 # # BslFI: # [GGGAC] # 1276 1769 # # BsmI: # [GAATGC] # 81 2964 # # BspCNI: # [CTCAG] # 193 227 343 2282 # # BspLU11I*: # [ACATGT] # 468 966 1611 # # BspMI: # [ACCTGC] # 1151 1894 # # BsrBI: # [CCGCTC] # 991 2054 # # BsrDI: # [GCAATG] # 475 1027 1543 # # BsrI: # [ACTGG] # 8 11 478 604 939 1005 # # BssKI: # [CCNGG] # 272 1074 1699 # # BstKTI: # [GATC] # 18 20 74 130 180 189 # 223 243 285 306 338 459 # 580 # # BtgZI: # [GCGATG] # 1484 1561 # # BtrI: # [CACGTC] # 923 2122 # # BtsI: # [GCAGTG] # 453 2592 # # Cac8I: # [GCNNGC] # 69 72 86 332 1146 1340 # # CauII*: # [CCSGG] # 270 2775 # # Cfr10I: # [RCCGGY] # 28 374 2643 # # CfrI: # [YGGCCR] # 986 2059 # # ClaI: # [ATCGAT] # 154 2891 # # Csp6I: # [GTAC] # 63 212 557 560 645 1008 # # CspCI: # [CAANNNNNGTGG] # 35 90 2920 # # CviAII: # [CATG] # 9 16 18 21 42 48 # 55 106 107 134 161 173 # 196 198 393 451 917 # # CviJI: # [RGCY] # 8 8 14 16 21 26 # 30 32 40 51 67 67 # 70 70 74 74 76 77 # 84 108 111 113 124 130 # 131 138 167 168 207 222 # 249 272 # # CviRI*: # [TGCA] # 7 12 26 57 63 333 # 396 461 544 1146 # # DdeI: # [CTNAG] # 193 240 322 2290 # # DpnI: # [GATC] # 18 20 74 130 180 189 # 222 244 285 306 338 459 # 580 # # DraII: # [RGGNCCY] # 142 2903 # # DraIII: # [CACNNNGTG] # 96 2949 # # DsaI*: # [CCRYGG] # 1325 1720 # # Eam1105I: # [GACNNNNNGTC] # 480 2565 # # EciI: # [GGCGGA] # 421 2624 # # Ecl136II: # [GAGCTC] # 1271 1774 # # Eco47III: # [AGCGCT] # 1160 1885 # # Eco57I: # [CTGAAG] # 66 72 131 198 333 372 # 586 1287 # # Eco57MI: # [CTGRAG] # 66 72 131 134 145 198 # 333 372 586 1008 # # EcoRII: # [CCWGG] # 1346 1699 # # EcoRV: # [GATATC] # 1438 1607 # # EcoT22I: # [ATGCAT] # 1008 2037 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 327 866 1788 # # FatI: # [CATG] # 9 16 18 21 42 48 # 54 106 107 134 161 173 # 196 198 393 451 918 # # FauI: # [CCCGC] # 492 2553 # # FokI: # [GGATG] # 140 258 517 696 1434 # # GlaI: # [GCGC] # 581 1160 1304 # # GsuI: # [CTGGAG] # 145 1164 1736 # # HaeII: # [RGCGCY] # 1162 1883 # # HaeIII: # [GGCC] # 86 144 299 459 597 688 # 772 # # HgaI: # [GACGC] # 145 264 2636 # # HgiAI*: # [GWGCWC] # 1269 1776 # # HgiJII*: # [GRGCYC] # 1269 1776 # # HhaI: # [GCGC] # 580 1161 1304 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 3 3 29 32 32 32 # 32 32 32 32 58 61 # 76 85 130 235 304 726 # 1111 # # Hin4II*: # [CCTTC] # 25 61 86 155 282 346 # 366 437 1287 # # Hin6I: # [GCGC] # 582 1159 1304 # # HindII: # [GTYRAC] # 5 58 127 469 525 920 # 941 # # HindIII: # [AAGCTT] # 108 273 565 2099 # # HinfI: # [GANTC] # 26 39 39 96 122 126 # 132 138 165 171 172 324 # 447 499 549 # # HpaI: # [GTTAAC] # 999 2046 # # HpaII: # [CCGG] # 29 271 374 2371 # # HphI: # [GGTGA] # 236 361 506 583 629 730 # # Hpy166II: # [GTNNAC] # 5 58 127 137 159 190 # 238 386 469 525 751 # # Hpy178III*: # [TCNNGA] # 35 54 94 95 138 235 # 248 325 369 451 479 522 # # Hpy188I: # [TCNGA] # 12 37 39 54 54 65 # 69 116 120 156 156 165 # 201 237 238 279 321 336 # 390 # # Hpy99I: # [CGWCG] # 3 69 133 540 815 1485 # # Ksp632I*: # [CTCTTC] # 26 50 111 142 207 541 # 838 1130 # # MaeI: # [CTAG] # 13 13 42 61 114 145 # 167 255 294 339 400 468 # 734 # # MaeII: # [ACGT] # 37 104 235 268 446 516 # 552 887 # # MaeIII: # [GTNAC] # 37 41 98 112 153 180 # 271 286 328 362 370 392 # 415 # # MboI: # [GATC] # 18 20 74 130 180 189 # 220 246 285 306 338 459 # 580 # # MboII: # [GAAGA] # 3 3 3 3 3 6 # 12 30 41 50 53 85 # 103 107 110 111 136 145 # 171 178 207 316 348 404 # 417 # # McrI*: # [CGRYCG] # 815 2230 # # MlyI: # [GAGTC] # 172 471 492 848 1062 # # MnlI: # [CCTC] # 3 3 11 12 27 36 # 51 58 59 59 86 90 # 97 99 119 139 142 189 # 201 204 238 321 396 405 # # MroNI: # [GCCGGC] # 402 2643 # # MseI: # [TTAA] # 58 96 152 195 195 209 # 215 226 348 357 481 513 # # MslI: # [CAYNNNNRTG] # 252 489 914 1390 # # MwoI: # [GCNNNNNNNGC] # 166 608 822 1449 # # NaeI: # [GCCGGC] # 404 2641 # # NcoI: # [CCATGG] # 1325 1720 # # NdeI: # [CATATG] # 907 2138 # # NheI: # [GCTAGC] # 1497 1548 # # NlaIII: # [CATG] # 9 16 18 21 42 48 # 58 106 107 134 161 173 # 196 198 393 451 914 # # NlaIV: # [GGNNCC] # 143 335 801 1766 # # NspBII*: # [CMGCKG] # 486 820 1739 # # NspI: # [RCATGY] # 58 414 456 962 1155 # # PfoI: # [TCCNGGA] # 1346 1699 # # PleI: # [GAGTC] # 172 472 492 848 1061 # # PsiI: # [TTATAA] # 1120 1925 # # PvuI: # [CGATCG] # 815 2230 # # PvuII: # [CAGCTG] # 486 820 1739 # # RsaI: # [GTAC] # 62 212 557 560 645 1009 # # SacI: # [GAGCTC] # 1269 1776 # # SapI: # [GCTCTTC] # 870 2175 # # ScaI: # [AGTACT] # 62 1009 1974 # # ScrFI: # [CCNGG] # 270 1074 1701 # # SduI: # [GDGCHC] # 1269 1776 # # SecI*: # [CCNNGG] # 271 705 744 1325 # # SetI: # [ASST] # 8 9 21 23 28 29 # 29 33 34 38 41 46 # 62 66 68 68 71 72 # 72 73 74 104 107 108 # 111 131 143 180 191 199 # 206 209 391 # # SfaNI: # [GCATC] # 374 627 969 1075 # # SfeI*: # [CTRYAG] # 1097 1948 # # SgrAI: # [CRCCGGYG] # 402 2643 # # SmlI: # [CTYRAG] # 992 2053 # # Sse232I*: # [CGCCGGCG] # 402 2643 # # SspI: # [AATATT] # 307 1036 1702 # # StyI: # [CCWWGG] # 744 976 1325 # # TaiI: # [ACGT] # 34 104 235 268 449 516 # 552 887 # # TaqI: # [TCGA] # 53 119 154 166 358 657 # 1538 # # TaqII: # [GACCGA] # 1125 1920 # # TatI: # [WGTACW] # 64 1007 1974 # # TauI: # [GCSGC] # 685 991 1369 # # TfiI: # [GAWTC] # 39 65 96 122 132 165 # 171 324 499 687 745 # # TseI: # [GCWGC] # 3 118 246 408 571 1699 # # TsoI: # [TARCCA] # 32 267 494 637 718 897 # # Tsp45I: # [GTSAC] # 37 41 98 153 180 271 # 286 328 415 482 754 # # Tsp4CI*: # [ACNGT] # 184 219 491 633 1518 # # TspDTI: # [ATGAA] # 12 34 35 42 63 92 # 97 99 102 129 144 150 # 152 162 175 183 184 186 # 216 219 279 290 # # TspEI: # [AATT] # 7 11 12 18 19 27 # 27 30 31 32 34 63 # 65 66 72 78 81 86 # 86 113 120 137 138 150 # 158 179 195 217 373 420 # # TspGWI: # [ACGGA] # 110 378 491 2066 # # TspRI: # [CASTGNN] # 298 350 453 638 1306 # # TstI: # [CACNNNNNNTCC] # 25 32 32 370 2586 # # XbaI: # [TCTAGA] # 741 2304 # # XhoII: # [RGATCY] # 725 942 1378 # # ZraI: # [GACGTC] # 36 3009 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3045 # Reported_sequences: 1 # Reported_hitcount: 728 #---------------------------------------