Restriction Fragments for YDR015C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YDR015C on chromosome IV from coordinates 478199 to 477960.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24739.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 14 18 - TspDTI ATGAA 4 2 . . 34 37 + CviJI RGCY 35 35 . . 35 41 + SapI GCTCTTC 42 45 . . 36 41 + Ksp632I* CTCTTC 42 45 . . 37 41 - MboII GAAGA 29 28 . . 40 44 - MboII GAAGA 32 31 . . 43 47 - MboII GAAGA 35 34 . . 47 52 + StyI CCWWGG 47 51 . . 47 52 + SecI* CCNNGG 47 51 . . 52 55 - MnlI CCTC 45 44 . . 55 58 + CviJI RGCY 56 56 . . 61 65 - MboII GAAGA 53 52 . . 63 67 - TspDTI ATGAA 53 51 . . 71 74 + SetI ASST 74 70 . . 69 73 - Hin4II* CCTTC 63 62 . . 84 87 + CviJI RGCY 85 85 . . 85 95 + MwoI GCNNNNNNNGC 91 88 . . 93 96 + SetI ASST 96 92 . . 93 96 + CviJI RGCY 94 94 . . 93 96 + AluI AGCT 94 94 . . 100 103 + TaqI TCGA 100 102 . . 104 110 + PpuMI RGGWCCY 105 108 . . 104 110 + DraII RGGNCCY 105 108 . . 104 108 + BslFI GGGAC 118 122 . . 104 109 + NlaIV GGNNCC 106 106 . . 102 105 - MnlI CCTC 95 94 . . 105 109 + BmgT120I GGNCC 106 107 . . 105 109 + AvaII GGWCC 105 108 . . 105 109 + AsuI* GGNCC 105 108 . . 107 110 + SetI ASST 110 106 . . 108 111 + MnlI CCTC 118 117 . . 111 116 + StyI CCWWGG 111 115 . . 111 116 + SecI* CCNNGG 111 115 . . 121 124 + DpnI GATC 122 122 . . 121 124 + BstKTI GATC 123 121 . . 121 124 + MboI GATC 120 124 . . 146 151 + AcyI GRCGYC 147 149 . . 150 155 + Hpy178III* TCNNGA 151 153 . . 147 151 - HgaI GACGC 136 141 . . 162 172 + BsiYI* CCNNNNNNNGG 168 165 . . 163 173 + BsiYI* CCNNNNNNNGG 169 166 . . 172 175 + CviJI RGCY 173 173 . . 177 180 + AciI CCGC 177 179 . . 176 181 - EciI GGCGGA 166 164 . . 182 185 + SetI ASST 185 181 . . 191 195 + DdeI CTNAG 191 194 . . 191 195 + BspCNI CTCAG 204 202 . . 191 195 + BseMII CTCAG 205 203 . . 192 196 + Hpy188I TCNGA 194 193 . . 228 231 + TspEI AATT 227 231 . . 235 238 + TspEI AATT 234 238 . . 237 240 + MseI TTAA 237 239 . . #--------------------------------------- # # # AciI: # [CCGC] # 63 177 # # AcyI: # [GRCGYC] # 93 147 # # AluI: # [AGCT] # 94 146 # # AsuI*: # [GGNCC] # 105 135 # # AvaII: # [GGWCC] # 105 135 # # BmgT120I: # [GGNCC] # 106 134 # # BseMII: # [CTCAG] # 35 205 # # BsiYI*: # [CCNNNNNNNGG] # 1 71 168 # # BslFI: # [GGGAC] # 118 122 # # BspCNI: # [CTCAG] # 36 204 # # BstKTI: # [GATC] # 117 123 # # CviJI: # [RGCY] # 9 21 29 35 67 79 # # DdeI: # [CTNAG] # 49 191 # # DpnI: # [GATC] # 118 122 # # DraII: # [RGGNCCY] # 105 135 # # EciI: # [GGCGGA] # 74 166 # # HgaI: # [GACGC] # 104 136 # # Hin4II*: # [CCTTC] # 63 177 # # Hpy178III*: # [TCNNGA] # 89 151 # # Hpy188I: # [TCNGA] # 46 194 # # Ksp632I*: # [CTCTTC] # 42 198 # # MboI: # [GATC] # 120 120 # # MboII: # [GAAGA] # 3 3 18 29 187 # # MnlI: # [CCTC] # 23 45 50 122 # # MseI: # [TTAA] # 3 237 # # MwoI: # [GCNNNNNNNGC] # 91 149 # # NlaIV: # [GGNNCC] # 106 134 # # PpuMI: # [RGGWCCY] # 105 135 # # SapI: # [GCTCTTC] # 42 198 # # SecI*: # [CCNNGG] # 47 64 129 # # SetI: # [ASST] # 14 22 55 74 75 # # StyI: # [CCWWGG] # 47 64 129 # # TaqI: # [TCGA] # 100 140 # # TspDTI: # [ATGAA] # 4 49 187 # # TspEI: # [AATT] # 6 7 227 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 240 # Reported_sequences: 1 # Reported_hitcount: 52 #---------------------------------------