Restriction Fragments for SSB1/YDL229W



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The currently selected gene/sequence is :

SSB1/YDL229W on chromosome IV from coordinates 44065 to 45906.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.29928.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 5 10 + Eco57MI CTGRAG 26 24 . . 5 10 + Eco57I CTGAAG 26 24 . . 9 12 + SetI ASST 12 8 . . 18 23 + StyI CCWWGG 18 22 . . 18 23 + SecI* CCNNGG 18 22 . . 21 24 + SetI ASST 24 20 . . 34 39 + ClaI ATCGAT 35 37 . . 35 38 + TaqI TCGA 35 37 . . 42 45 + SetI ASST 45 41 . . 44 47 + Csp6I GTAC 44 46 . . 44 47 + RsaI GTAC 45 45 . . 49 52 + SetI ASST 52 48 . . 71 81 + BsaXI ACNNNNNCTCC 61 58 91 88 73 77 + TfiI GAWTC 73 76 . . 73 77 + HinfI GANTC 73 76 . . 77 80 + MnlI CCTC 87 86 . . 79 83 - TspGWI ACGGA 69 67 . . 87 90 + TspEI AATT 86 90 . . 105 108 + SetI ASST 108 104 . . 107 111 + MaeIII GTNAC 106 111 . . 113 117 + PleI GAGTC 121 122 . . 113 117 + MlyI GAGTC 122 122 . . 113 117 + HinfI GANTC 113 116 . . 115 119 + Tsp45I GTSAC 114 119 . . 115 119 + MaeIII GTNAC 114 119 . . 116 120 - HphI GGTGA 108 107 . . 121 125 + BccI CCATC 129 130 . . 141 146 + Hpy178III* TCNNGA 142 144 . . 145 149 + MboII GAAGA 157 156 . . 140 145 - Eco57MI CTGRAG 125 123 . . 140 145 - GsuI CTGGAG 125 123 . . 148 161 + AjuI GAANNNNNNNTTGG 140 135 172 167 160 164 + HphI GGTGA 172 171 . . 166 170 + BlsI GCNGC 168 167 . . 166 170 + BisI GCNGC 167 168 . . 166 170 + TseI GCWGC 166 169 . . 163 167 - SfaNI GCATC 153 157 . . 166 170 - BbvI GCAGC 153 157 . . 180 183 + SetI ASST 183 179 . . 180 183 + CviJI RGCY 181 181 . . 180 183 + AluI AGCT 181 181 . . 181 185 + BlsI GCNGC 183 182 . . 181 185 + BisI GCNGC 182 183 . . 181 185 + TseI GCWGC 181 184 . . 181 185 - BbvI GCAGC 168 172 . . 201 207 + TspRI CASTGNN 207 198 . . 202 206 + Tsp4CI* ACNGT 204 203 . . 209 212 + TaqI TCGA 209 211 . . 206 210 - MboII GAAGA 198 197 . . 205 210 - BbvII* GAAGAC 198 202 . . 214 220 + EspI* GCTNAGC 215 218 . . 211 215 - SfaNI GCATC 201 205 . . 215 219 + DdeI CTNAG 215 218 . . 233 237 + MboII GAAGA 245 244 . . 236 240 + TfiI GAWTC 236 239 . . 236 240 + HinfI GANTC 236 239 . . 239 242 + TaqI TCGA 239 241 . . 240 244 + Hpy99I CGWCG 244 239 . . 241 251 + Hin4I GAYNNNNNVTC 232 227 264 259 243 247 + Hpy99I CGWCG 247 242 . . 247 251 + TfiI GAWTC 247 250 . . 247 251 + HinfI GANTC 247 250 . . 247 256 + XmnI GAANNNNTTC 251 251 . . 241 251 - Hin4I GAYNNNNNVTC 264 259 232 227 264 267 + FatI CATG 263 267 . . 264 267 + NlaIII CATG 267 263 . . 264 267 + CviAII CATG 264 266 . . 265 269 + TspDTI ATGAA 280 278 . . 267 271 + MboII GAAGA 279 278 . . 267 272 + BbvII* GAAGAC 274 278 . . 271 274 + SetI ASST 274 270 . . 272 276 + BssKI CCNGG 271 276 . . 272 276 + BseBI CCWGG 273 274 . . 272 276 + ScrFI CCNGG 273 274 . . 272 276 + EcoRII CCWGG 271 276 . . 275 278 + CviJI RGCY 276 276 . . 275 278 + HaeIII GGCC 276 276 . . 284 287 + SetI ASST 287 283 . . 290 293 + TaqI TCGA 290 292 . . 291 295 + Hpy99I CGWCG 295 290 . . 292 297 + ZraI GACGTC 294 294 . . 292 297 + AcyI GRCGYC 293 295 . . 292 297 + AatII GACGTC 296 292 . . 293 296 + TaiI ACGT 296 292 . . 293 296 + SetI ASST 296 292 . . 293 296 + MaeII ACGT 293 295 . . 294 298 + Hpy99I CGWCG 298 293 . . 296 299 + TaqI TCGA 296 298 . . 301 307 + BstEII GGTNACC 301 306 . . 302 306 + MaeIII GTNAC 301 306 . . 298 302 - BccI CCATC 292 293 . . 307 311 - BsrI ACTGG 307 305 . . 306 311 - BfiI ACTGGG 301 300 . . 314 317 + TaqI TCGA 314 316 . . 321 331 + BsiYI* CCNNNNNNNGG 327 324 . . 321 331 + PflMI CCANNNNNTGG 327 324 . . 331 335 + MboII GAAGA 343 342 . . 359 364 + ApoI RAATTY 359 363 . . 360 363 + TspEI AATT 359 363 . . 365 368 + AciI CCGC 365 367 . . 385 389 + TspDTI ATGAA 400 398 . . 387 391 - Hin4II* CCTTC 381 380 . . 393 396 + TspEI AATT 392 396 . . 398 403 + Eco57MI CTGRAG 419 417 . . 398 403 + Eco57I CTGAAG 419 417 . . 400 413 + AjuI GAANNNNNNNTTGG 392 387 424 419 402 405 + SetI ASST 405 401 . . 402 405 + CviJI RGCY 403 403 . . 402 405 + AluI AGCT 403 403 . . 404 408 + DdeI CTNAG 404 407 . . 419 422 + SetI ASST 422 418 . . 417 421 - Hin4II* CCTTC 411 410 . . 429 432 + CviJI RGCY 430 430 . . 429 432 + HaeIII GGCC 430 430 . . 430 434 - BceAI ACGGC 415 417 . . 439 443 + Tsp4CI* ACNGT 441 440 . . 444 449 + GsaI CCCAGC 448 444 . . 444 449 + BseYI CCCAGC 444 448 . . 442 446 - BslFI GGGAC 427 431 . . 447 450 + SetI ASST 450 446 . . 447 450 + CviJI RGCY 448 448 . . 447 450 + AluI AGCT 448 448 . . 455 458 + MseI TTAA 455 457 . . 459 463 + Hpy99I CGWCG 463 458 . . 460 464 + HgaI GACGC 469 474 . . 474 477 + SetI ASST 477 473 . . 474 477 + CviJI RGCY 475 475 . . 474 477 + AluI AGCT 475 475 . . 479 484 + StyI CCWWGG 479 483 . . 479 484 + SecI* CCNNGG 479 483 . . 483 487 + FokI GGATG 496 500 . . 483 487 + BseGI GGATG 489 487 . . 487 492 + Cfr10I RCCGGY 487 491 . . 484 488 - SfaNI GCATC 474 478 . . 488 491 + HpaII CCGG 488 490 . . 490 495 + NlaIV GGNNCC 492 492 . . 490 495 + HgiCI* GGYRCC 490 494 . . 508 513 + AclI AACGTT 509 511 . . 509 512 + TaiI ACGT 512 508 . . 509 512 + SetI ASST 512 508 . . 509 512 + MaeII ACGT 509 511 . . 528 539 + BcgI CGANNNNNNTGC 517 515 551 549 531 534 + SetI ASST 534 530 . . 538 542 + TauI GCSGC 541 538 . . 538 542 + BlsI GCNGC 540 539 . . 538 542 + BisI GCNGC 539 540 . . 539 544 + NspBII* CMGCKG 541 541 . . 539 542 + AciI CCGC 539 541 . . 541 551 + MwoI GCNNNNNNNGC 547 544 . . 541 545 + BlsI GCNGC 543 542 . . 541 545 + BisI GCNGC 542 543 . . 541 545 + TseI GCWGC 541 544 . . 541 545 - BbvI GCAGC 528 532 . . 554 558 + Tsp4CI* ACNGT 556 555 . . 559 562 + MaeI CTAG 559 561 . . 561 564 + SetI ASST 564 560 . . 574 578 + Hpy188I TCNGA 576 575 . . 588 593 + BspLU11I* ACATGT 588 592 . . 588 593 + NspI RCATGY 592 588 . . 588 593 + AflIII ACRYGT 588 592 . . 589 592 + FatI CATG 588 592 . . 589 592 + NlaIII CATG 592 588 . . 589 592 + CviAII CATG 589 591 . . 602 605 + TaqI TCGA 602 604 . . 608 613 - TaqII CACCCA 598 596 . . 617 620 + Csp6I GTAC 617 619 . . 617 620 + RsaI GTAC 618 618 . . 623 626 + TaqI TCGA 623 625 . . 638 641 + CviRI* TGCA 639 639 . . 642 647 - BsrDI GCAATG 641 639 . . 655 660 + Hpy166II GTNNAC 657 657 . . 660 666 + TspRI CASTGNN 666 657 . . 661 665 + Tsp4CI* ACNGT 663 662 . . 665 668 + MseI TTAA 665 667 . . 676 681 + BetI* WCCGGW 676 680 . . 677 680 + HpaII CCGG 677 679 . . 680 684 + MaeIII GTNAC 679 684 . . 688 697 + MslI CAYNNNNRTG 692 692 . . 688 697 + OliI CACNNNNGTG 692 692 . . 692 697 - TaqII CACCCA 682 680 . . 699 704 + Hpy178III* TCNNGA 700 702 . . 707 710 + TaqI TCGA 707 709 . . 713 724 + BstXI CCANNNNNNTGG 720 716 . . 720 725 - MmeI TCCRAC 701 699 . . 735 738 + CviJI RGCY 736 736 . . 739 744 + EcoRI GAATTC 739 743 . . 739 744 + ApoI RAATTY 739 743 . . 740 743 + TspEI AATT 739 743 . . 743 748 + Hpy178III* TCNNGA 744 746 . . 747 751 + MboII GAAGA 759 758 . . 750 754 + MboII GAAGA 762 761 . . 750 755 + BbvII* GAAGAC 757 761 . . 750 763 + AjuI GAANNNNNNNTTGG 742 737 774 769 754 758 + BsrI ACTGG 759 757 . . 769 774 + MmeI TCCRAC 794 792 . . 769 773 + Hpy188I TCNGA 771 770 . . 771 775 + Hpy99I CGWCG 775 770 . . 775 779 - SfaNI GCATC 765 769 . . 783 786 + SetI ASST 786 782 . . 783 786 + CviJI RGCY 784 784 . . 783 786 + AluI AGCT 784 784 . . 791 795 + MboII GAAGA 803 802 . . 805 809 + BlsI GCNGC 807 806 . . 805 809 + BisI GCNGC 806 807 . . 805 809 + TseI GCWGC 805 808 . . 803 808 - EcoP15I CAGCAG 775 777 . . 808 818 + MwoI GCNNNNNNNGC 814 811 . . 805 809 - BbvI GCAGC 792 796 . . 806 811 - EcoP15I CAGCAG 778 780 . . 816 819 + SetI ASST 819 815 . . 816 819 + CviJI RGCY 817 817 . . 816 819 + AluI AGCT 817 817 . . 818 822 + DdeI CTNAG 818 821 . . 820 830 + FalI AAGNNNNNCTT 811 806 843 838 826 829 + SetI ASST 829 825 . . 820 830 - FalI AAGNNNNNCTT 843 838 811 806 832 836 - MboII GAAGA 824 823 . . 838 842 + Tsp45I GTSAC 837 842 . . 838 842 + MaeIII GTNAC 837 842 . . 850 854 + Tsp4CI* ACNGT 852 851 . . 859 864 + Hpy166II GTNNAC 861 861 . . 859 864 + HindII GTYRAC 861 861 . . 862 866 + HinfI GANTC 862 865 . . 862 866 - PleI GAGTC 856 857 . . 862 866 - MlyI GAGTC 856 856 . . 875 879 + Tsp4CI* ACNGT 877 876 . . 877 881 + HphI GGTGA 889 888 . . 880 884 + MboII GAAGA 892 891 . . 883 893 + Hin4I GAYNNNNNVTC 874 869 906 901 886 891 + AsuII TTCGAA 887 889 . . 887 890 + TaqI TCGA 887 889 . . 889 893 + TfiI GAWTC 889 892 . . 889 893 + HinfI GANTC 889 892 . . 893 896 + MnlI CCTC 903 902 . . 883 893 - Hin4I GAYNNNNNVTC 906 901 874 869 902 905 + MaeI CTAG 902 904 . . 906 909 + SetI ASST 909 905 . . 906 909 + CviJI RGCY 907 907 . . 906 909 + AluI AGCT 907 907 . . 908 911 + MaeI CTAG 908 910 . . 916 920 + MboII GAAGA 928 927 . . 916 921 + BbvII* GAAGAC 923 927 . . 928 932 + TauI GCSGC 931 928 . . 928 932 + BlsI GCNGC 930 929 . . 928 932 + BisI GCNGC 929 930 . . 929 932 + AciI CCGC 929 931 . . 942 947 + Hpy166II GTNNAC 944 944 . . 942 947 + AccI GTMKAC 943 945 . . 951 956 + NlaIV GGNNCC 953 953 . . 954 957 + SetI ASST 957 953 . . 975 979 + FokI GGATG 988 992 . . 975 979 + BseGI GGATG 981 979 . . 972 976 - Hin4II* CCTTC 966 965 . . 976 980 - SfaNI GCATC 966 970 . . 980 984 + DdeI CTNAG 980 983 . . 983 988 + XhoII RGATCY 983 987 . . 983 988 + BglII AGATCT 983 987 . . 984 987 + DpnI GATC 985 985 . . 984 987 + BstKTI GATC 986 984 . . 984 987 + MboI GATC 983 987 . . 989 993 + DdeI CTNAG 989 992 . . 993 997 + BsmAI GTCTC 998 1002 . . 1001 1004 + TaqI TCGA 1001 1003 . . 1002 1006 + Hpy99I CGWCG 1006 1001 . . 1003 1014 + DrdI GACNNNNNNGTC 1009 1007 . . 1024 1029 + NlaIV GGNNCC 1026 1026 . . 1029 1040 + TstI CACNNNNNNTCC 1020 1015 1052 1047 1034 1039 + EcoRI GAATTC 1034 1038 . . 1034 1039 + ApoI RAATTY 1034 1038 . . 1035 1038 + TspEI AATT 1034 1038 . . 1043 1046 + SetI ASST 1046 1042 . . 1044 1048 + BmgT120I GGNCC 1045 1046 . . 1044 1048 + AvaII GGWCC 1044 1047 . . 1044 1048 + AsuI* GGNCC 1044 1047 . . 1060 1064 + Hpy188I TCNGA 1062 1061 . . 1073 1077 + Tsp4CI* ACNGT 1075 1074 . . 1081 1086 + MfeI CAATTG 1081 1085 . . 1082 1085 + TspEI AATT 1081 1085 . . 1097 1100 + MseI TTAA 1097 1099 . . 1106 1110 + TspDTI ATGAA 1121 1119 . . 1110 1113 + SetI ASST 1113 1109 . . 1110 1113 + CviJI RGCY 1111 1111 . . 1110 1113 + AluI AGCT 1111 1111 . . 1117 1127 + MwoI GCNNNNNNNGC 1123 1120 . . 1121 1125 + Tsp4CI* ACNGT 1123 1122 . . 1126 1130 + BlsI GCNGC 1128 1127 . . 1126 1130 + BisI GCNGC 1127 1128 . . 1126 1130 + TseI GCWGC 1126 1129 . . 1126 1130 - BbvI GCAGC 1113 1117 . . 1127 1132 - EcoP15I CAGCAG 1099 1101 . . 1137 1140 + SetI ASST 1140 1136 . . 1145 1150 + Hpy178III* TCNNGA 1146 1148 . . 1150 1155 + Cfr10I RCCGGY 1150 1154 . . 1151 1154 + HpaII CCGG 1151 1153 . . 1152 1157 + CfrI YGGCCR 1152 1156 . . 1153 1156 + CviJI RGCY 1154 1154 . . 1153 1156 + HaeIII GGCC 1154 1154 . . 1165 1169 + Hpy188I TCNGA 1167 1166 . . 1175 1180 + StyI CCWWGG 1175 1179 . . 1175 1180 + SecI* CCNNGG 1175 1179 . . 1213 1216 + MaeI CTAG 1213 1215 . . 1215 1218 + SetI ASST 1218 1214 . . 1226 1229 + CviRI* TGCA 1227 1227 . . 1230 1233 + SetI ASST 1233 1229 . . 1231 1235 + HphI GGTGA 1243 1242 . . 1232 1236 + Tsp45I GTSAC 1231 1236 . . 1232 1236 + MaeIII GTNAC 1231 1236 . . 1235 1240 + BspLU11I* ACATGT 1235 1239 . . 1235 1240 + NspI RCATGY 1239 1235 . . 1235 1240 + AflIII ACRYGT 1235 1239 . . 1236 1239 + FatI CATG 1235 1239 . . 1236 1239 + NlaIII CATG 1239 1235 . . 1236 1239 + CviAII CATG 1236 1238 . . 1263 1274 + TstI CACNNNNNNTCC 1254 1249 1286 1281 1267 1271 + Tsp4CI* ACNGT 1269 1268 . . 1272 1277 + MmeI TCCRAC 1297 1295 . . 1277 1281 + BccI CCATC 1285 1286 . . 1280 1285 + Hpy178III* TCNNGA 1281 1283 . . 1286 1290 + MboII GAAGA 1298 1297 . . 1286 1298 + BarI GAAGNNNNNNTAC 1278 1273 1310 1305 1289 1301 + PsrI GAACNNNNNNTAC 1281 1276 1313 1308 1291 1294 + SetI ASST 1294 1290 . . 1300 1305 + BspLU11I* ACATGT 1300 1304 . . 1300 1305 + NspI RCATGY 1304 1300 . . 1300 1305 + AflIII ACRYGT 1300 1304 . . 1301 1304 + FatI CATG 1300 1304 . . 1301 1304 + NlaIII CATG 1304 1300 . . 1301 1304 + CviAII CATG 1301 1303 . . 1321 1325 + Tsp4CI* ACNGT 1323 1322 . . 1314 1327 - AjuI GAANNNNNNNTTGG 1339 1334 1307 1302 1328 1331 + TspEI AATT 1327 1331 . . 1336 1341 + Hpy166II GTNNAC 1338 1338 . . 1336 1341 + AccI GTMKAC 1337 1339 . . 1333 1337 - BsrI ACTGG 1333 1331 . . 1332 1337 - BfiI ACTGGG 1327 1326 . . 1341 1346 + StyI CCWWGG 1341 1345 . . 1341 1346 + SecI* CCNNGG 1341 1345 . . 1344 1347 + SetI ASST 1347 1343 . . 1345 1349 + HphI GGTGA 1357 1356 . . 1346 1351 + Hpy166II GTNNAC 1348 1348 . . 1350 1353 + TaiI ACGT 1353 1349 . . 1350 1353 + SetI ASST 1353 1349 . . 1350 1353 + MaeII ACGT 1350 1352 . . 1350 1355 + AflIII ACRYGT 1350 1354 . . 1354 1359 + Hpy166II GTNNAC 1356 1356 . . 1354 1359 + HpaI GTTAAC 1356 1356 . . 1354 1359 + HindII GTYRAC 1356 1356 . . 1355 1358 + MseI TTAA 1355 1357 . . 1358 1362 + Tsp4CI* ACNGT 1360 1359 . . 1381 1385 + HphI GGTGA 1393 1392 . . 1379 1384 - TaqII CACCCA 1369 1367 . . 1384 1389 + EcoRI GAATTC 1384 1388 . . 1384 1389 + ApoI RAATTY 1384 1388 . . 1385 1388 + TspEI AATT 1384 1388 . . 1388 1391 + TaqI TCGA 1388 1390 . . 1395 1399 + MboII GAAGA 1407 1406 . . 1401 1410 + MslI CAYNNNNRTG 1405 1405 . . 1401 1405 - FokI GGATG 1387 1391 . . 1401 1405 - BseGI GGATG 1400 1398 . . 1413 1418 + Cac8I GCNNGC 1415 1415 . . 1410 1414 - SfaNI GCATC 1400 1404 . . 1415 1420 + PvuII CAGCTG 1417 1417 . . 1415 1420 + NspBII* CMGCKG 1417 1417 . . 1416 1419 + SetI ASST 1419 1415 . . 1416 1419 + CviJI RGCY 1417 1417 . . 1416 1419 + AluI AGCT 1417 1417 . . 1420 1424 + HphI GGTGA 1432 1431 . . 1421 1426 + Hpy166II GTNNAC 1423 1423 . . 1426 1430 - BsrI ACTGG 1426 1424 . . 1437 1440 + SetI ASST 1440 1436 . . 1437 1440 + CviJI RGCY 1438 1438 . . 1437 1440 + AluI AGCT 1438 1438 . . 1444 1449 + AsuII TTCGAA 1445 1447 . . 1445 1448 + TaqI TCGA 1445 1447 . . 1442 1446 - MboII GAAGA 1434 1433 . . 1446 1457 + BcgI CGANNNNNNTGC 1435 1433 1469 1467 1453 1457 - SfaNI GCATC 1443 1447 . . 1460 1464 + Tsp4CI* ACNGT 1462 1461 . . 1466 1471 + Hpy178III* TCNNGA 1467 1469 . . 1472 1475 + SetI ASST 1475 1471 . . 1474 1478 + MaeIII GTNAC 1473 1478 . . 1470 1474 - Hin4II* CCTTC 1464 1463 . . 1482 1486 + Hpy99I CGWCG 1486 1481 . . 1484 1487 + TaqI TCGA 1484 1486 . . 1480 1484 - BceAI ACGGC 1465 1467 . . 1491 1496 + Hpy166II GTNNAC 1493 1493 . . 1491 1496 + AccI GTMKAC 1492 1494 . . 1495 1500 + Cfr10I RCCGGY 1495 1499 . . 1495 1500 + BetI* WCCGGW 1495 1499 . . 1495 1500 + AgeI ACCGGT 1495 1499 . . 1496 1499 + HpaII CCGG 1496 1498 . . 1504 1508 - MboII GAAGA 1496 1495 . . 1503 1508 - BbvII* GAAGAC 1496 1500 . . 1546 1550 + Hpy188I TCNGA 1548 1547 . . 1543 1547 - MboII GAAGA 1535 1534 . . 1547 1552 + Eco57MI CTGRAG 1568 1566 . . 1542 1547 - BbvII* GAAGAC 1535 1539 . . 1535 1547 - BarI GAAGNNNNNNTAC 1559 1554 1527 1522 1547 1552 + Eco57I CTGAAG 1568 1566 . . 1549 1553 + MboII GAAGA 1561 1560 . . 1554 1557 + TspEI AATT 1553 1557 . . 1567 1572 + Hpy166II GTNNAC 1569 1569 . . 1567 1572 + HpaI GTTAAC 1569 1569 . . 1567 1572 + HindII GTYRAC 1569 1569 . . 1563 1567 - BccI CCATC 1557 1558 . . 1568 1571 + MseI TTAA 1568 1570 . . 1569 1574 + TsoI TARCCA 1585 1583 . . 1565 1570 - TsoI TARCCA 1555 1553 . . 1575 1578 + SetI ASST 1578 1574 . . 1575 1578 + CviJI RGCY 1576 1576 . . 1575 1578 + AluI AGCT 1576 1576 . . 1577 1582 + Eco57MI CTGRAG 1598 1596 . . 1577 1582 + Eco57I CTGAAG 1598 1596 . . 1579 1583 + MboII GAAGA 1591 1590 . . 1579 1584 - Ksp632I* CTCTTC 1574 1577 . . 1590 1593 + CviJI RGCY 1591 1591 . . 1591 1595 + BlsI GCNGC 1593 1592 . . 1591 1595 + BisI GCNGC 1592 1593 . . 1591 1595 + TseI GCWGC 1591 1594 . . 1594 1604 + MwoI GCNNNNNNNGC 1600 1597 . . 1591 1595 - BbvI GCAGC 1578 1582 . . 1598 1602 + TspDTI ATGAA 1613 1611 . . 1601 1606 + HindIII AAGCTT 1601 1605 . . 1602 1605 + SetI ASST 1605 1601 . . 1602 1605 + CviJI RGCY 1603 1603 . . 1602 1605 + AluI AGCT 1603 1603 . . 1623 1626 + SetI ASST 1626 1622 . . 1623 1626 + CviJI RGCY 1624 1624 . . 1623 1626 + AluI AGCT 1624 1624 . . 1625 1628 + MaeI CTAG 1625 1627 . . 1634 1643 + BsaBI GATNNNNATC 1638 1638 . . 1639 1643 + TfiI GAWTC 1639 1642 . . 1639 1643 + HinfI GANTC 1639 1642 . . 1646 1649 + TaiI ACGT 1649 1645 . . 1646 1649 + SetI ASST 1649 1645 . . 1646 1649 + MaeII ACGT 1646 1648 . . 1646 1656 + BsaXI ACNNNNNCTCC 1636 1633 1666 1663 1652 1655 + MnlI CCTC 1662 1661 . . 1655 1659 + BccI CCATC 1663 1664 . . 1658 1661 + TaqI TCGA 1658 1660 . . 1666 1670 + Tsp4CI* ACNGT 1668 1667 . . 1669 1673 + Tsp45I GTSAC 1668 1673 . . 1669 1673 + MaeIII GTNAC 1668 1673 . . 1671 1677 + TspRI CASTGNN 1677 1668 . . 1675 1683 + Tth111I GACNNNGTC 1678 1679 . . 1678 1682 - BsrI ACTGG 1678 1676 . . 1677 1682 - BfiI ACTGGG 1672 1671 . . 1687 1691 - MboII GAAGA 1679 1678 . . 1686 1691 - BbvII* GAAGAC 1679 1683 . . 1694 1697 + TspEI AATT 1693 1697 . . 1698 1702 + MboII GAAGA 1710 1709 . . 1698 1703 - Ksp632I* CTCTTC 1693 1696 . . 1704 1707 + SetI ASST 1707 1703 . . 1705 1710 + NlaIV GGNNCC 1707 1707 . . 1703 1706 - MnlI CCTC 1696 1695 . . 1725 1728 + SetI ASST 1728 1724 . . 1725 1728 + CviJI RGCY 1726 1726 . . 1725 1728 + AluI AGCT 1726 1726 . . 1726 1730 + BlsI GCNGC 1728 1727 . . 1726 1730 + BisI GCNGC 1727 1728 . . 1726 1730 + TseI GCWGC 1726 1729 . . 1726 1730 - BbvI GCAGC 1713 1717 . . 1735 1739 + Hpy188I TCNGA 1737 1736 . . 1741 1751 + MwoI GCNNNNNNNGC 1747 1744 . . 1738 1742 - SfaNI GCATC 1728 1732 . . 1746 1749 + CviJI RGCY 1747 1747 . . 1747 1751 + BlsI GCNGC 1749 1748 . . 1747 1751 + BisI GCNGC 1748 1749 . . 1747 1751 + TseI GCWGC 1747 1750 . . 1747 1751 - BbvI GCAGC 1734 1738 . . 1754 1757 + CviRI* TGCA 1755 1755 . . 1760 1763 + TaqI TCGA 1760 1762 . . 1762 1766 + MboII GAAGA 1774 1773 . . 1762 1767 + BbvII* GAAGAC 1769 1773 . . 1768 1772 + BccI CCATC 1776 1777 . . 1772 1777 - EcoP15I CAGCAG 1744 1746 . . 1778 1782 + TspDTI ATGAA 1793 1791 . . 1781 1784 + TspEI AATT 1780 1784 . . 1791 1794 + CviJI RGCY 1792 1792 . . 1793 1798 + Eco57MI CTGRAG 1814 1812 . . 1793 1798 + Eco57I CTGAAG 1814 1812 . . 1806 1810 + MboII GAAGA 1818 1817 . . 1806 1811 - Ksp632I* CTCTTC 1801 1804 . . 1816 1820 + Tsp45I GTSAC 1815 1820 . . 1816 1820 + MaeIII GTNAC 1815 1820 . . 1820 1825 + StyI CCWWGG 1820 1824 . . 1820 1825 + SecI* CCNNGG 1820 1824 . . 1817 1821 - HphI GGTGA 1809 1808 . . 1824 1827 + CviJI RGCY 1825 1825 . . 1824 1827 + HaeIII GGCC 1825 1825 . . 1827 1830 + FatI CATG 1826 1830 . . 1827 1830 + NlaIII CATG 1830 1826 . . 1827 1830 + CviAII CATG 1827 1829 . . 1831 1835 - MboII GAAGA 1823 1822 . . 1830 1835 - BbvII* GAAGAC 1823 1827 . . 1839 1842 + MseI TTAA 1839 1841 . . #--------------------------------------- # # # AatII: # [GACGTC] # 296 1546 # # AccI: # [GTMKAC] # 155 350 394 943 # # AciI: # [CCGC] # 174 365 390 913 # # AclI: # [AACGTT] # 509 1333 # # AcyI: # [GRCGYC] # 293 1549 # # AflIII: # [ACRYGT] # 50 65 492 588 647 # # AgeI: # [ACCGGT] # 347 1495 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 32 32 140 220 # 318 503 533 # # AluI: # [AGCT] # 21 21 27 27 33 45 # 90 102 116 138 181 204 # 222 306 309 # # ApoI: # [RAATTY] # 295 350 359 380 458 # # AsuI*: # [GGNCC] # 798 1044 # # AsuII: # [TTCGAA] # 397 558 887 # # AvaII: # [GGWCC] # 798 1044 # # BarI: # [GAAGNNNNNNTAC] # 32 32 217 283 1278 # # BbvI: # [GCAGC] # 15 21 108 135 153 264 # 321 360 465 # # BbvII*: # [GAAGAC] # 19 39 54 76 90 144 # 166 198 483 573 # # BccI: # [CCATC] # 66 106 113 129 163 272 # 993 # # BceAI: # [ACGGC] # 377 415 1050 # # BcgI: # [CGANNNNNNTGC] # 34 34 373 517 884 # # BetI*: # [WCCGGW] # 347 676 819 # # BfiI: # [ACTGGG] # 170 301 345 1026 # # BglII: # [AGATCT] # 859 983 # # BisI: # [GCNGC] # 3 15 21 94 123 135 # 167 198 264 357 465 # # BlsI: # [GCNGC] # 3 15 21 93 123 135 # 168 198 264 357 465 # # BmgT120I: # [GGNCC] # 797 1045 # # BsaBI: # [GATNNNNATC] # 204 1638 # # BsaXI: # [ACNNNNNCTCC] # 30 30 61 176 1545 # # BseBI: # [CCWGG] # 273 1569 # # BseGI: # [GGATG] # 419 442 489 492 # # BseYI: # [CCCAGC] # 444 1398 # # BsiYI*: # [CCNNNNNNNGG] # 327 1515 # # BslFI: # [GGGAC] # 427 1415 # # BsmAI: # [GTCTC] # 844 998 # # BspLU11I*: # [ACATGT] # 65 542 588 647 # # BsrDI: # [GCAATG] # 641 1201 # # BsrI: # [ACTGG] # 93 164 252 307 452 574 # # BssKI: # [CCNGG] # 271 1571 # # BstEII: # [GGTNACC] # 301 1541 # # BstKTI: # [GATC] # 856 986 # # BstXI: # [CCANNNNNNTGG] # 720 1122 # # Cac8I: # [GCNNGC] # 427 1415 # # Cfr10I: # [RCCGGY] # 345 347 487 663 # # CfrI: # [YGGCCR] # 690 1152 # # ClaI: # [ATCGAT] # 35 1807 # # Csp6I: # [GTAC] # 44 573 1225 # # CviAII: # [CATG] # 15 65 264 325 526 647 # # CviJI: # [RGCY] # 4 12 15 17 18 21 # 21 21 27 27 33 33 # 43 45 48 90 95 102 # 127 138 177 204 261 263 # # CviRI*: # [TGCA] # 87 528 588 639 # # DdeI: # [CTNAG] # 9 162 189 215 414 853 # # DpnI: # [GATC] # 857 985 # # DrdI: # [GACNNNNNNGTC] # 833 1009 # # Eco57I: # [CTGAAG] # 26 28 30 216 393 1149 # # Eco57MI: # [CTGRAG] # 26 28 30 99 216 294 # 1149 # # EcoP15I: # [CAGCAG] # 3 98 321 645 775 # # EcoRI: # [GAATTC] # 295 350 458 739 # # EcoRII: # [CCWGG] # 271 1571 # # EspI*: # [GCTNAGC] # 215 1627 # # FalI: # [AAGNNNNNCTT] # 0 0 32 811 999 # # FatI: # [CATG] # 16 65 263 325 526 647 # # FokI: # [GGATG] # 399 455 492 496 # # GsaI: # [CCCAGC] # 448 1394 # # GsuI: # [CTGGAG] # 125 1717 # # HaeIII: # [GGCC] # 17 154 276 671 724 # # HgaI: # [GACGC] # 469 1373 # # HgiCI*: # [GGYRCC] # 490 1352 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 232 610 936 # # Hin4II*: # [CCTTC] # 30 378 381 498 555 # # HindII: # [GTYRAC] # 213 273 495 861 # # HindIII: # [AAGCTT] # 241 1601 # # HinfI: # [GANTC] # 11 27 40 73 123 203 # 615 750 # # HpaI: # [GTTAAC] # 213 273 1356 # # HpaII: # [CCGG] # 189 345 346 474 488 # # HphI: # [GGTGA] # 33 36 39 64 108 114 # 354 377 717 # # Hpy166II: # [GTNNAC] # 8 10 67 70 76 83 # 204 273 394 657 # # Hpy178III*: # [TCNNGA] # 44 135 142 186 375 402 # 558 # # Hpy188I: # [TCNGA] # 105 105 189 195 291 381 # 576 # # Hpy99I: # [CGWCG] # 3 3 48 165 231 244 # 312 356 480 # # Ksp632I*: # [CTCTTC] # 41 108 119 1574 # # MaeI: # [CTAG] # 6 217 305 343 412 559 # # MaeII: # [ACGT] # 196 216 293 296 841 # # MaeIII: # [GTNAC] # 8 27 106 147 158 187 # 195 242 378 394 # # MboI: # [GATC] # 859 983 # # MboII: # [GAAGA] # 3 5 19 21 26 27 # 30 31 34 36 39 41 # 41 44 47 62 64 64 # 68 88 109 157 370 416 # # MfeI: # [CAATTG] # 761 1081 # # MlyI: # [GAGTC] # 122 734 986 # # MmeI: # [TCCRAC] # 93 503 545 701 # # MnlI: # [CCTC] # 34 87 146 759 816 # # MseI: # [TTAA] # 3 210 213 258 271 432 # 455 # # MslI: # [CAYNNNNRTG] # 437 692 713 # # MwoI: # [GCNNNNNNNGC] # 95 147 267 309 477 547 # # NlaIII: # [CATG] # 12 65 267 325 526 647 # # NlaIV: # [GGNNCC] # 73 135 461 492 681 # # NspBII*: # [CMGCKG] # 425 541 876 # # NspI: # [RCATGY] # 65 538 592 647 # # OliI: # [CACNNNNGTG] # 692 1150 # # PflMI: # [CCANNNNNTGG] # 327 1515 # # PleI: # [GAGTC] # 121 735 986 # # PsrI: # [GAACNNNNNNTAC] # 32 529 1281 # # PvuII: # [CAGCTG] # 425 1417 # # RsaI: # [GTAC] # 45 573 1224 # # ScrFI: # [CCNGG] # 273 1569 # # SecI*: # [CCNNGG] # 18 22 166 461 479 696 # # SetI: # [ASST] # 6 7 9 10 12 12 # 13 15 17 21 21 21 # 21 22 23 27 27 27 # 28 30 33 35 35 48 # 53 56 58 61 66 67 # 75 78 80 89 91 103 # 109 114 222 # # SfaNI: # [GCATC] # 43 48 114 153 201 273 # 285 291 434 # # StyI: # [CCWWGG] # 18 22 166 461 479 696 # # TaiI: # [ACGT] # 193 216 296 296 841 # # TaqI: # [TCGA] # 6 18 21 30 35 39 # 51 57 82 84 102 114 # 174 174 180 288 387 # # TaqII: # [CACCCA] # 84 473 598 687 # # TauI: # [GCSGC] # 390 541 911 # # TfiI: # [GAWTC] # 11 73 163 203 642 750 # # TseI: # [GCWGC] # 15 21 95 135 166 264 # 321 360 465 # # TsoI: # [TARCCA] # 30 257 1555 # # Tsp45I: # [GTSAC] # 27 114 147 394 437 723 # # Tsp4CI*: # [ACNGT] # 25 37 48 54 102 107 # 115 146 174 189 198 204 # 206 237 # # TspDTI: # [ATGAA] # 49 120 180 280 492 721 # # TspEI: # [AATT] # 33 47 57 62 86 87 # 140 169 246 273 295 347 # # TspGWI: # [ACGGA] # 69 1773 # # TspRI: # [CASTGNN] # 165 207 459 1011 # # TstI: # [CACNNNNNNTCC] # 32 32 202 556 1020 # # Tth111I: # [GACNNNGTC] # 164 1678 # # XhoII: # [RGATCY] # 859 983 # # XmnI: # [GAANNNNTTC] # 251 1591 # # ZraI: # [GACGTC] # 294 1548 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1842 # Reported_sequences: 1 # Reported_hitcount: 497 #---------------------------------------