Restriction Fragments for CDC13/YDL220C



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The currently selected gene/sequence is :

CDC13/YDL220C on chromosome IV from coordinates 65018 to 62244.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15641.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 10 + SetI ASST 10 6 . . 9 13 + DdeI CTNAG 9 12 . . 13 17 + MboII GAAGA 25 24 . . 17 20 + CviJI RGCY 18 18 . . 13 18 - Ksp632I* CTCTTC 8 11 . . 13 19 - SapI GCTCTTC 8 11 . . 20 24 + DdeI CTNAG 20 23 . . 20 24 - BspCNI CTCAG 12 10 . . 20 24 - BseMII CTCAG 11 9 . . 28 31 + MnlI CCTC 38 37 . . 54 59 + SacI GAGCTC 58 54 . . 54 59 + Ecl136II GAGCTC 56 56 . . 54 59 + SduI GDGCHC 58 54 . . 54 59 + HgiJII* GRGCYC 58 54 . . 54 59 + HgiAI* GWGCWC 58 54 . . 55 58 + SetI ASST 58 54 . . 55 58 + CviJI RGCY 56 56 . . 55 58 + AluI AGCT 56 56 . . 56 63 + PspXI VCTCGAGB 57 61 . . 57 62 + SmlI CTYRAG 57 61 . . 57 62 + XhoI CTCGAG 57 61 . . 57 62 + BmeT110I CYCGRG 58 60 . . 57 62 + AvaI CYCGRG 57 61 . . 58 61 + TaqI TCGA 58 60 . . 76 79 + CviJI RGCY 77 77 . . 73 77 - Hin4II* CCTTC 67 66 . . 77 87 + MwoI GCNNNNNNNGC 83 80 . . 82 87 + GsaI CCCAGC 86 82 . . 82 87 + BseYI CCCAGC 82 86 . . 104 107 + CviRI* TGCA 105 105 . . 107 110 + TspEI AATT 106 110 . . 114 119 + HaeII RGCGCY 118 114 . . 115 118 + Hin6I GCGC 115 117 . . 115 118 + GlaI GCGC 116 116 . . 115 118 + HhaI GCGC 117 115 . . 121 124 + MseI TTAA 121 123 . . 124 127 + SetI ASST 127 123 . . 125 128 + MnlI CCTC 135 134 . . 134 137 + SetI ASST 137 133 . . 157 160 + MaeI CTAG 157 159 . . 160 163 + CviJI RGCY 161 161 . . 169 172 + TspEI AATT 168 172 . . 177 180 - MnlI CCTC 170 169 . . 182 187 + BseRI GAGGAG 197 195 . . 182 185 - MnlI CCTC 175 174 . . 187 190 + DpnI GATC 188 188 . . 187 190 + BstKTI GATC 189 187 . . 187 190 + MboI GATC 186 190 . . 199 203 + MboII GAAGA 211 210 . . 202 205 + DpnI GATC 203 203 . . 202 205 + BstKTI GATC 204 202 . . 202 205 + MboI GATC 201 205 . . 206 211 + SspI AATATT 208 208 . . 211 214 + MseI TTAA 211 213 . . 220 225 + Eco57MI CTGRAG 241 239 . . 220 225 + Eco57I CTGAAG 241 239 . . 232 242 + Hin4I GAYNNNNNVTC 223 218 255 250 237 242 + SduI GDGCHC 241 237 . . 237 242 + HgiJII* GRGCYC 241 237 . . 238 241 + CviJI RGCY 239 239 . . 241 245 + Hpy188I TCNGA 243 242 . . 247 250 + SetI ASST 250 246 . . 246 249 - MnlI CCTC 239 238 . . 252 255 + CviJI RGCY 253 253 . . 254 258 + BssKI CCNGG 253 258 . . 254 258 + ScrFI CCNGG 255 256 . . 254 258 + CauII* CCSGG 255 256 . . 255 258 + HpaII CCGG 255 257 . . 257 261 + BinI* GGATC 265 266 . . 258 261 + DpnI GATC 259 259 . . 258 261 + BstKTI GATC 260 258 . . 258 261 + MboI GATC 257 261 . . 310 314 + TfiI GAWTC 310 313 . . 310 314 + HinfI GANTC 310 313 . . 310 320 + Hin4I GAYNNNNNVTC 301 296 333 328 313 317 + Hpy188I TCNGA 315 314 . . 316 320 + HinfI GANTC 316 319 . . 318 322 + DdeI CTNAG 318 321 . . 318 322 + BspCNI CTCAG 331 329 . . 318 322 + BseMII CTCAG 332 330 . . 316 320 - PleI GAGTC 310 311 . . 316 320 - MlyI GAGTC 310 310 . . 323 326 + Hin6I GCGC 323 325 . . 323 326 + GlaI GCGC 324 324 . . 323 326 + HhaI GCGC 325 323 . . 325 329 + SfaNI GCATC 334 338 . . 326 330 - FokI GGATG 312 316 . . 326 330 - BseGI GGATG 325 323 . . 334 338 + Tsp4CI* ACNGT 336 335 . . 343 347 + DdeI CTNAG 343 346 . . 343 347 - BspCNI CTCAG 335 333 . . 343 347 - BseMII CTCAG 334 332 . . 356 359 + SetI ASST 359 355 . . 354 358 - HphI GGTGA 346 345 . . 364 367 + SetI ASST 367 363 . . 386 391 + HindIII AAGCTT 386 390 . . 387 390 + SetI ASST 390 386 . . 387 390 + CviJI RGCY 388 388 . . 387 390 + AluI AGCT 388 388 . . 426 430 + DdeI CTNAG 426 429 . . 430 433 - MnlI CCTC 423 422 . . 441 445 + Tsp4CI* ACNGT 443 442 . . 444 449 - MmeI TCCRAC 425 423 . . 484 487 + TaqI TCGA 484 486 . . 494 498 + BlsI GCNGC 496 495 . . 494 498 + BisI GCNGC 495 496 . . 494 498 + BbvI GCAGC 506 510 . . 494 498 + TseI GCWGC 494 497 . . 497 502 + Cac8I GCNNGC 499 499 . . 501 506 - BsmI GAATGC 501 499 . . 511 515 + Hpy188I TCNGA 513 512 . . 523 527 + Hpy188I TCNGA 525 524 . . 553 558 + EcoRV GATATC 555 555 . . 557 561 - MboII GAAGA 549 548 . . 565 568 + TspEI AATT 564 568 . . 568 571 + MseI TTAA 568 570 . . 577 581 + TspDTI ATGAA 592 590 . . 584 588 - BsmAI GTCTC 578 582 . . 584 589 - Esp3I CGTCTC 578 582 . . 598 601 + SetI ASST 601 597 . . 601 606 + AhaIII* TTTAAA 603 603 . . 602 605 + MseI TTAA 602 604 . . 604 609 + ApoI RAATTY 604 608 . . 605 608 + TspEI AATT 604 608 . . 618 622 + TfiI GAWTC 618 621 . . 618 622 + HinfI GANTC 618 621 . . 651 656 + Hpy178III* TCNNGA 652 654 . . 657 667 + BsiYI* CCNNNNNNNGG 663 660 . . 665 668 + SetI ASST 668 664 . . 687 690 + CviJI RGCY 688 688 . . 688 692 + TauI GCSGC 691 688 . . 688 692 + BlsI GCNGC 690 689 . . 688 692 + BisI GCNGC 689 690 . . 689 692 + AciI CCGC 689 691 . . 691 695 + BlsI GCNGC 693 692 . . 691 695 + BisI GCNGC 692 693 . . 691 695 + BbvI GCAGC 703 707 . . 691 695 + TseI GCWGC 691 694 . . 696 699 + TspEI AATT 695 699 . . 697 702 + VspI ATTAAT 698 700 . . 698 701 + MseI TTAA 698 700 . . 724 727 + MaeI CTAG 724 726 . . 732 735 + SetI ASST 735 731 . . 728 733 - BciVI GTATCC 722 721 . . 742 746 + TfiI GAWTC 742 745 . . 742 746 + HinfI GANTC 742 745 . . 754 759 + EcoRI GAATTC 754 758 . . 754 759 + ApoI RAATTY 754 758 . . 755 758 + TspEI AATT 754 758 . . 765 769 + BlsI GCNGC 767 766 . . 765 769 + BisI GCNGC 766 767 . . 765 769 + BbvI GCAGC 777 781 . . 765 769 + TseI GCWGC 765 768 . . 767 770 + SetI ASST 770 766 . . 767 770 + CviJI RGCY 768 768 . . 767 770 + AluI AGCT 768 768 . . 768 778 + MwoI GCNNNNNNNGC 774 771 . . 774 778 + HgaI GACGC 783 788 . . 779 782 + MseI TTAA 779 781 . . 788 792 + TspDTI ATGAA 803 801 . . 813 816 + FatI CATG 812 816 . . 813 816 + NlaIII CATG 816 812 . . 813 816 + CviAII CATG 813 815 . . 820 823 - MnlI CCTC 813 812 . . 826 831 + EcoT22I ATGCAT 830 826 . . 827 830 + CviRI* TGCA 828 828 . . 829 832 + FatI CATG 828 832 . . 829 832 + NlaIII CATG 832 828 . . 829 832 + CviAII CATG 829 831 . . 836 839 + Hin6I GCGC 836 838 . . 836 839 + GlaI GCGC 837 837 . . 836 839 + HhaI GCGC 838 836 . . 840 845 + XhoII RGATCY 840 844 . . 841 844 + DpnI GATC 842 842 . . 841 844 + BstKTI GATC 843 841 . . 841 844 + MboI GATC 840 844 . . 841 845 - BinI* GGATC 835 836 . . 853 857 + TfiI GAWTC 853 856 . . 850 853 - MnlI CCTC 843 842 . . 853 857 + HinfI GANTC 853 856 . . 856 860 + Hpy188I TCNGA 858 857 . . 859 863 + TfiI GAWTC 859 862 . . 859 863 + HinfI GANTC 859 862 . . 863 866 + MnlI CCTC 873 872 . . 871 874 + SetI ASST 874 870 . . 872 875 + MnlI CCTC 882 881 . . 875 878 + MnlI CCTC 885 884 . . 875 885 + BsiYI* CCNNNNNNNGG 881 878 . . 873 878 - BseRI GAGGAG 864 862 . . 881 885 + BssKI CCNGG 880 885 . . 881 884 + HpaII CCGG 881 883 . . 881 885 + ScrFI CCNGG 882 883 . . 881 885 + CauII* CCSGG 882 883 . . 885 894 + XmnI GAANNNNTTC 889 889 . . 887 892 + SspI AATATT 889 889 . . 895 898 + SetI ASST 898 894 . . 895 898 + CviJI RGCY 896 896 . . 895 898 + AluI AGCT 896 896 . . 909 913 - FokI GGATG 895 899 . . 909 913 - BseGI GGATG 908 906 . . 915 919 + Tsp45I GTSAC 914 919 . . 915 919 + MaeIII GTNAC 914 919 . . 912 916 - BsrI ACTGG 912 910 . . 924 927 + SetI ASST 927 923 . . 937 947 + BaeI ACNNNNGTAYC 926 921 959 954 943 946 + Csp6I GTAC 943 945 . . 943 946 + RsaI GTAC 944 944 . . 946 957 + BstXI CCANNNNNNTGG 953 949 . . 952 955 + TspEI AATT 951 955 . . 958 967 + MslI CAYNNNNRTG 962 962 . . 967 971 + TfiI GAWTC 967 970 . . 967 971 + HinfI GANTC 967 970 . . 970 975 + Hpy178III* TCNNGA 971 973 . . 970 975 + BetI* WCCGGW 970 974 . . 970 975 + BspMII* TCCGGA 970 974 . . 971 974 + HpaII CCGG 971 973 . . 981 984 - MnlI CCTC 974 973 . . 984 988 + MboII GAAGA 996 995 . . 984 989 - Ksp632I* CTCTTC 979 982 . . 989 998 + XmnI GAANNNNTTC 993 993 . . 991 996 + HindIII AAGCTT 991 995 . . 992 995 + SetI ASST 995 991 . . 992 995 + CviJI RGCY 993 993 . . 992 995 + AluI AGCT 993 993 . . 1004 1008 + Tsp4CI* ACNGT 1006 1005 . . 1008 1012 + Hpy188I TCNGA 1010 1009 . . 1011 1022 + PpiI GAACNNNNNCTC 1003 998 1035 1030 1013 1018 + MluI ACGCGT 1013 1017 . . 1013 1018 + AflIII ACRYGT 1013 1017 . . 1014 1017 + FnuDII* CGCG 1015 1015 . . 1019 1022 + MnlI CCTC 1029 1028 . . 1015 1019 - HgaI GACGC 1004 1009 . . 1026 1029 + AciI CCGC 1026 1028 . . 1032 1035 + CviJI RGCY 1033 1033 . . 1034 1038 + BccI CCATC 1042 1043 . . 1057 1060 + MaeI CTAG 1057 1059 . . 1059 1062 + SetI ASST 1062 1058 . . 1059 1062 + CviJI RGCY 1060 1060 . . 1059 1062 + AluI AGCT 1060 1060 . . 1061 1064 + MaeI CTAG 1061 1063 . . 1069 1072 + CviJI RGCY 1070 1070 . . 1071 1075 + DdeI CTNAG 1071 1074 . . 1071 1075 + BspCNI CTCAG 1084 1082 . . 1071 1075 + BseMII CTCAG 1085 1083 . . 1081 1084 + SetI ASST 1084 1080 . . 1087 1090 - MnlI CCTC 1080 1079 . . 1095 1098 + TspEI AATT 1094 1098 . . 1104 1107 + FatI CATG 1103 1107 . . 1104 1107 + NlaIII CATG 1107 1103 . . 1104 1107 + CviAII CATG 1104 1106 . . 1105 1109 + TspDTI ATGAA 1120 1118 . . 1107 1111 + TfiI GAWTC 1107 1110 . . 1107 1111 + HinfI GANTC 1107 1110 . . 1113 1116 + SetI ASST 1116 1112 . . 1114 1117 + MnlI CCTC 1124 1123 . . 1118 1121 + TspEI AATT 1117 1121 . . 1124 1128 - BsrI ACTGG 1124 1122 . . 1142 1147 + SspI AATATT 1144 1144 . . 1147 1150 + CviRI* TGCA 1148 1148 . . 1157 1160 + TspEI AATT 1156 1160 . . 1170 1174 + BlsI GCNGC 1172 1171 . . 1170 1174 + BisI GCNGC 1171 1172 . . 1170 1174 + BbvI GCAGC 1182 1186 . . 1170 1174 + TseI GCWGC 1170 1173 . . 1172 1175 + SetI ASST 1175 1171 . . 1172 1175 + CviJI RGCY 1173 1173 . . 1172 1175 + AluI AGCT 1173 1173 . . 1188 1198 + BsiYI* CCNNNNNNNGG 1194 1191 . . 1186 1190 - BslFI GGGAC 1171 1175 . . 1192 1200 + Tth111I GACNNNGTC 1195 1196 . . 1196 1199 + SetI ASST 1199 1195 . . 1196 1202 + PpuMI RGGWCCY 1197 1200 . . 1196 1202 + DraII RGGNCCY 1197 1200 . . 1197 1201 + BmgT120I GGNCC 1198 1199 . . 1197 1201 + AvaII GGWCC 1197 1200 . . 1197 1201 + AsuI* GGNCC 1197 1200 . . 1200 1204 + BssKI CCNGG 1199 1204 . . 1200 1204 + BseBI CCWGG 1201 1202 . . 1200 1204 + ScrFI CCNGG 1201 1202 . . 1200 1204 + EcoRII CCWGG 1199 1204 . . 1203 1208 + NlaIV GGNNCC 1205 1205 . . 1203 1208 + HgiCI* GGYRCC 1203 1207 . . 1207 1211 + BssKI CCNGG 1206 1211 . . 1207 1211 + BseBI CCWGG 1208 1209 . . 1207 1211 + ScrFI CCNGG 1208 1209 . . 1207 1211 + EcoRII CCWGG 1206 1211 . . 1210 1215 + AcyI GRCGYC 1211 1213 . . 1211 1221 + MwoI GCNNNNNNNGC 1217 1214 . . 1215 1220 + MfeI CAATTG 1215 1219 . . 1211 1215 - HgaI GACGC 1200 1205 . . 1216 1219 + TspEI AATT 1215 1219 . . 1219 1222 + CviRI* TGCA 1220 1220 . . 1227 1230 + DpnI GATC 1228 1228 . . 1227 1230 + BstKTI GATC 1229 1227 . . 1227 1236 + BsaBI GATNNNNATC 1231 1231 . . 1227 1230 + MboI GATC 1226 1230 . . 1220 1231 - BcgI CGANNNNNNTGC 1243 1241 1209 1207 1235 1240 + Hpy178III* TCNNGA 1236 1238 . . 1238 1242 + Hpy99I CGWCG 1242 1237 . . 1250 1254 + TfiI GAWTC 1250 1253 . . 1250 1254 + HinfI GANTC 1250 1253 . . 1244 1254 - Hin4I GAYNNNNNVTC 1267 1262 1235 1230 1258 1263 + SacI GAGCTC 1262 1258 . . 1258 1263 + Ecl136II GAGCTC 1260 1260 . . 1258 1263 + SduI GDGCHC 1262 1258 . . 1258 1263 + HgiJII* GRGCYC 1262 1258 . . 1258 1263 + HgiAI* GWGCWC 1262 1258 . . 1259 1262 + SetI ASST 1262 1258 . . 1259 1262 + CviJI RGCY 1260 1260 . . 1259 1262 + AluI AGCT 1260 1260 . . 1260 1266 + SapI GCTCTTC 1267 1270 . . 1261 1266 + Ksp632I* CTCTTC 1267 1270 . . 1262 1266 - MboII GAAGA 1254 1253 . . 1272 1277 + AhaIII* TTTAAA 1274 1274 . . 1273 1276 + MseI TTAA 1273 1275 . . 1277 1281 + Tsp4CI* ACNGT 1279 1278 . . 1288 1298 + Hin4I GAYNNNNNVTC 1279 1274 1311 1306 1293 1298 + EcoRV GATATC 1295 1295 . . 1297 1301 + Hpy188I TCNGA 1299 1298 . . 1288 1298 - Hin4I GAYNNNNNVTC 1311 1306 1279 1274 1311 1315 + BsrI ACTGG 1316 1314 . . 1319 1322 + CviJI RGCY 1320 1320 . . 1324 1327 + DpnI GATC 1325 1325 . . 1324 1327 + BstKTI GATC 1326 1324 . . 1324 1327 + MboI GATC 1323 1327 . . 1326 1329 + TaqI TCGA 1326 1328 . . 1328 1333 + ApoI RAATTY 1328 1332 . . 1329 1332 + TspEI AATT 1328 1332 . . 1335 1339 + Hpy99I CGWCG 1339 1334 . . 1333 1337 - TspGWI ACGGA 1323 1321 . . 1337 1340 + TaqI TCGA 1337 1339 . . 1349 1354 + XhoII RGATCY 1349 1353 . . 1349 1354 + NlaIV GGNNCC 1351 1351 . . 1349 1354 + BamHI GGATCC 1349 1353 . . 1349 1353 + BinI* GGATC 1357 1358 . . 1350 1353 + DpnI GATC 1351 1351 . . 1350 1353 + BstKTI GATC 1352 1350 . . 1350 1353 + MboI GATC 1349 1353 . . 1350 1354 - BinI* GGATC 1344 1345 . . 1358 1362 + BslFI GGGAC 1372 1376 . . 1373 1376 + AciI CCGC 1373 1375 . . 1375 1380 + Cac8I GCNNGC 1377 1377 . . 1376 1390 + XcmI CCANNNNNNNNNTGG 1383 1382 . . 1378 1381 + CviJI RGCY 1379 1379 . . 1380 1385 + SecI* CCNNGG 1380 1384 . . 1380 1390 + BsiYI* CCNNNNNNNGG 1386 1383 . . 1381 1385 + BssKI CCNGG 1380 1385 . . 1381 1385 + BseBI CCWGG 1382 1383 . . 1381 1385 + ScrFI CCNGG 1382 1383 . . 1381 1385 + EcoRII CCWGG 1380 1385 . . 1383 1386 + SetI ASST 1386 1382 . . 1404 1408 + MboII GAAGA 1416 1415 . . 1425 1430 + Cac8I GCNNGC 1427 1427 . . 1429 1432 + Hin6I GCGC 1429 1431 . . 1429 1432 + GlaI GCGC 1430 1430 . . 1429 1432 + HhaI GCGC 1431 1429 . . 1431 1436 + Cac8I GCNNGC 1433 1433 . . 1435 1438 + Hin6I GCGC 1435 1437 . . 1435 1438 + GlaI GCGC 1436 1436 . . 1435 1438 + HhaI GCGC 1437 1435 . . 1439 1442 + SetI ASST 1442 1438 . . 1441 1446 + BsgI GTGCAG 1462 1460 . . 1442 1445 + CviRI* TGCA 1443 1443 . . 1437 1442 - BspMI ACCTGC 1428 1432 . . 1443 1454 + AlfI GCANNNNNNTGC 1432 1430 1466 1464 1437 1443 - AarI CACCTGC 1428 1432 . . 1445 1448 + SetI ASST 1448 1444 . . 1446 1451 + NlaIV GGNNCC 1448 1448 . . 1446 1451 + HgiCI* GGYRCC 1446 1450 . . 1443 1448 - BspMI ACCTGC 1434 1438 . . 1449 1454 + Cac8I GCNNGC 1451 1451 . . 1443 1449 - AarI CACCTGC 1434 1438 . . 1452 1457 + MstI* TGCGCA 1454 1454 . . 1453 1456 + Hin6I GCGC 1453 1455 . . 1453 1456 + GlaI GCGC 1454 1454 . . 1453 1456 + HhaI GCGC 1455 1453 . . 1443 1454 - AlfI GCANNNNNNTGC 1466 1464 1432 1430 1460 1464 + Hpy99I CGWCG 1464 1459 . . 1464 1469 + AcyI GRCGYC 1465 1467 . . 1465 1469 - HgaI GACGC 1454 1459 . . 1471 1476 + ClaI ATCGAT 1472 1474 . . 1472 1475 + TaqI TCGA 1472 1474 . . 1474 1477 + DpnI GATC 1475 1475 . . 1474 1477 + BstKTI GATC 1476 1474 . . 1474 1477 + MboI GATC 1473 1477 . . 1478 1483 + Esp3I CGTCTC 1484 1488 . . 1474 1478 - BinI* GGATC 1468 1469 . . 1479 1483 + BsmAI GTCTC 1484 1488 . . 1476 1480 - TspGWI ACGGA 1466 1464 . . 1485 1494 + MslI CAYNNNNRTG 1489 1489 . . 1490 1494 + FokI GGATG 1503 1507 . . 1490 1494 + BseGI GGATG 1496 1494 . . 1520 1524 + BccI CCATC 1528 1529 . . 1523 1526 + TaqI TCGA 1523 1525 . . 1525 1530 + EcoRI GAATTC 1525 1529 . . 1525 1530 + ApoI RAATTY 1525 1529 . . 1526 1529 + TspEI AATT 1525 1529 . . 1539 1543 + BslFI GGGAC 1553 1557 . . 1541 1545 + HinfI GANTC 1541 1544 . . 1544 1547 + TaqI TCGA 1544 1546 . . 1541 1545 - PleI GAGTC 1535 1536 . . 1541 1545 - MlyI GAGTC 1535 1535 . . 1555 1559 - BsmAI GTCTC 1549 1553 . . 1555 1560 - Eco31I GGTCTC 1549 1553 . . 1566 1575 + MslI CAYNNNNRTG 1570 1570 . . 1580 1585 + NspI RCATGY 1584 1580 . . 1580 1585 + Cac8I GCNNGC 1582 1582 . . 1580 1585 + SphI GCATGC 1584 1580 . . 1581 1584 + FatI CATG 1580 1584 . . 1581 1584 + NlaIII CATG 1584 1580 . . 1581 1584 + CviAII CATG 1581 1583 . . 1585 1588 + MaeI CTAG 1585 1587 . . 1588 1592 + BsmAI GTCTC 1593 1597 . . 1608 1611 + SetI ASST 1611 1607 . . 1608 1613 + BspMI ACCTGC 1617 1621 . . 1651 1656 + EcoRV GATATC 1653 1653 . . 1679 1684 + EcoRV GATATC 1681 1681 . . 1709 1712 + SetI ASST 1712 1708 . . 1709 1712 + CviJI RGCY 1710 1710 . . 1709 1712 + AluI AGCT 1710 1710 . . 1717 1720 - MnlI CCTC 1710 1709 . . 1720 1723 + CviJI RGCY 1721 1721 . . 1728 1731 + CviJI RGCY 1729 1729 . . 1730 1734 + BccI CCATC 1738 1739 . . 1734 1737 + FatI CATG 1733 1737 . . 1734 1737 + NlaIII CATG 1737 1733 . . 1734 1737 + CviAII CATG 1734 1736 . . 1736 1741 + TatI WGTACW 1736 1740 . . 1736 1741 + Bsp1407I TGTACA 1736 1740 . . 1737 1740 + Csp6I GTAC 1737 1739 . . 1737 1740 + RsaI GTAC 1738 1738 . . 1748 1751 + TspEI AATT 1747 1751 . . 1750 1755 + AsuII TTCGAA 1751 1753 . . 1751 1754 + TaqI TCGA 1751 1753 . . 1755 1760 + AclI AACGTT 1756 1758 . . 1756 1759 + TaiI ACGT 1759 1755 . . 1756 1759 + SetI ASST 1759 1755 . . 1756 1759 + MaeII ACGT 1756 1758 . . 1762 1766 + HinfI GANTC 1762 1765 . . 1762 1766 - PleI GAGTC 1756 1757 . . 1762 1766 - MlyI GAGTC 1756 1756 . . 1766 1770 + DdeI CTNAG 1766 1769 . . 1769 1772 + SetI ASST 1772 1768 . . 1769 1772 + CviJI RGCY 1770 1770 . . 1769 1772 + AluI AGCT 1770 1770 . . 1771 1775 + DdeI CTNAG 1771 1774 . . 1771 1775 + BspCNI CTCAG 1784 1782 . . 1771 1775 + BseMII CTCAG 1785 1783 . . 1772 1776 + Hpy188I TCNGA 1774 1773 . . 1779 1784 + SspI AATATT 1781 1781 . . 1793 1796 + CviJI RGCY 1794 1794 . . 1795 1799 + DdeI CTNAG 1795 1798 . . 1800 1805 + XhoII RGATCY 1800 1804 . . 1800 1805 + BglII AGATCT 1800 1804 . . 1801 1804 + DpnI GATC 1802 1802 . . 1801 1804 + BstKTI GATC 1803 1801 . . 1801 1804 + MboI GATC 1800 1804 . . 1804 1809 + SfeI* CTRYAG 1804 1808 . . 1811 1815 + BceAI ACGGC 1827 1829 . . 1812 1817 + McrI* CGRYCG 1815 1813 . . 1812 1817 + XmaIII* CGGCCG 1812 1816 . . 1812 1817 + CfrI YGGCCR 1812 1816 . . 1813 1816 + CviJI RGCY 1814 1814 . . 1813 1816 + HaeIII GGCC 1814 1814 . . 1814 1818 + TauI GCSGC 1817 1814 . . 1814 1818 + BlsI GCNGC 1816 1815 . . 1814 1818 + BisI GCNGC 1815 1816 . . 1815 1818 + AciI CCGC 1815 1817 . . 1816 1819 + FnuDII* CGCG 1817 1817 . . 1817 1822 + BtgZI GCGATG 1832 1836 . . 1820 1824 + TspDTI ATGAA 1835 1833 . . 1826 1829 + SetI ASST 1829 1825 . . 1838 1842 + BceAI ACGGC 1854 1856 . . 1855 1859 + TspDTI ATGAA 1870 1868 . . 1874 1879 + TatI WGTACW 1874 1878 . . 1874 1879 + Bsp1407I TGTACA 1874 1878 . . 1875 1878 + Csp6I GTAC 1875 1877 . . 1875 1878 + RsaI GTAC 1876 1876 . . 1880 1884 + BceAI ACGGC 1896 1898 . . 1886 1889 + TspEI AATT 1885 1889 . . 1890 1895 + BsmI GAATGC 1896 1894 . . 1902 1905 + Hin6I GCGC 1902 1904 . . 1902 1905 + GlaI GCGC 1903 1903 . . 1902 1905 + HhaI GCGC 1904 1902 . . 1903 1906 + FnuDII* CGCG 1904 1904 . . 1913 1916 + CviJI RGCY 1914 1914 . . 1914 1924 + MwoI GCNNNNNNNGC 1920 1917 . . 1914 1919 + Cfr10I RCCGGY 1914 1918 . . 1915 1918 + HpaII CCGG 1915 1917 . . 1917 1922 + NlaIV GGNNCC 1919 1919 . . 1917 1921 + BmgT120I GGNCC 1918 1919 . . 1917 1921 + AvaII GGWCC 1917 1920 . . 1917 1921 + AsuI* GGNCC 1917 1920 . . 1920 1924 + FauI CCCGC 1928 1930 . . 1921 1924 + AciI CCGC 1921 1923 . . 1918 1922 - BslFI GGGAC 1903 1907 . . 1923 1928 - BsmI GAATGC 1923 1921 . . 1937 1941 + BlsI GCNGC 1939 1938 . . 1937 1941 + BisI GCNGC 1938 1939 . . 1937 1941 + BbvI GCAGC 1949 1953 . . 1937 1941 + TseI GCWGC 1937 1940 . . 1940 1944 + SfaNI GCATC 1949 1953 . . 1946 1949 + MnlI CCTC 1956 1955 . . 1941 1946 - BtgZI GCGATG 1926 1930 . . 1951 1955 + Hin4II* CCTTC 1961 1960 . . 1956 1960 + Tsp4CI* ACNGT 1958 1957 . . 1957 1963 + TspRI CASTGNN 1963 1954 . . 1969 1972 + MseI TTAA 1969 1971 . . 1994 1999 + Hpy178III* TCNNGA 1995 1997 . . 2001 2004 + TspEI AATT 2000 2004 . . 2008 2012 + TfiI GAWTC 2008 2011 . . 2008 2012 + HinfI GANTC 2008 2011 . . 2012 2015 + AciI CCGC 2012 2014 . . 2016 2019 + TspEI AATT 2015 2019 . . 2029 2034 + AsuII TTCGAA 2030 2032 . . 2030 2033 + TaqI TCGA 2030 2032 . . 2032 2036 + MboII GAAGA 2044 2043 . . 2037 2040 + Csp6I GTAC 2037 2039 . . 2032 2037 - Ksp632I* CTCTTC 2027 2030 . . 2037 2040 + RsaI GTAC 2038 2038 . . 2039 2044 + BetI* WCCGGW 2039 2043 . . 2040 2043 + HpaII CCGG 2040 2042 . . 2044 2047 + SetI ASST 2047 2043 . . 2043 2046 - MnlI CCTC 2036 2035 . . 2063 2072 + XmnI GAANNNNTTC 2067 2067 . . 2066 2070 + Tsp4CI* ACNGT 2068 2067 . . 2078 2083 + Hpy178III* TCNNGA 2079 2081 . . 2078 2083 + NruI TCGCGA 2080 2080 . . 2079 2082 + FnuDII* CGCG 2080 2080 . . 2086 2090 + DdeI CTNAG 2086 2089 . . 2089 2092 + SetI ASST 2092 2088 . . 2086 2090 - BspCNI CTCAG 2078 2076 . . 2086 2090 - BseMII CTCAG 2077 2075 . . 2088 2091 - MnlI CCTC 2081 2080 . . 2093 2096 + TaqI TCGA 2093 2095 . . 2096 2100 + TspDTI ATGAA 2111 2109 . . 2107 2110 + FatI CATG 2106 2110 . . 2107 2110 + NlaIII CATG 2110 2106 . . 2107 2110 + CviAII CATG 2107 2109 . . 2108 2112 + TspDTI ATGAA 2123 2121 . . 2123 2128 + TaqII CACCCA 2139 2137 . . 2122 2126 - HphI GGTGA 2114 2113 . . 2136 2139 + SetI ASST 2139 2135 . . 2136 2139 + CviJI RGCY 2137 2137 . . 2136 2139 + AluI AGCT 2137 2137 . . 2157 2168 + BcgI CGANNNNNNTGC 2146 2144 2180 2178 2159 2162 + SetI ASST 2162 2158 . . 2163 2168 + BsmI GAATGC 2169 2167 . . 2166 2169 + CviRI* TGCA 2167 2167 . . 2170 2180 + Hin4I GAYNNNNNVTC 2161 2156 2193 2188 2171 2174 + TaiI ACGT 2174 2170 . . 2171 2174 + SetI ASST 2174 2170 . . 2171 2174 + MaeII ACGT 2171 2173 . . 2171 2181 + BsaXI ACNNNNNCTCC 2161 2158 2191 2188 2171 2176 + AflIII ACRYGT 2171 2175 . . 2170 2175 - BtrI CACGTC 2172 2172 . . 2177 2180 + MnlI CCTC 2187 2186 . . 2180 2184 + Hin4II* CCTTC 2190 2189 . . 2183 2186 + TaqI TCGA 2183 2185 . . 2193 2198 + Hpy166II GTNNAC 2195 2195 . . 2206 2210 - MboII GAAGA 2198 2197 . . 2218 2222 + HinfI GANTC 2218 2221 . . 2218 2222 - PleI GAGTC 2212 2213 . . 2222 2225 + MnlI CCTC 2232 2231 . . 2218 2222 - MlyI GAGTC 2212 2212 . . 2222 2227 + SecI* CCNNGG 2222 2226 . . 2220 2225 - BseRI GAGGAG 2211 2209 . . 2226 2229 + CviJI RGCY 2227 2227 . . 2227 2232 + Cac8I GCNNGC 2229 2229 . . 2223 2228 - NmeAIII GCCGAG 2203 2201 . . 2231 2241 + MwoI GCNNNNNNNGC 2237 2234 . . 2232 2235 + MnlI CCTC 2242 2241 . . 2235 2239 + FauI CCCGC 2243 2245 . . 2236 2239 + AciI CCGC 2236 2238 . . 2237 2240 + FnuDII* CGCG 2238 2238 . . 2238 2241 + Hin6I GCGC 2238 2240 . . 2238 2241 + GlaI GCGC 2239 2239 . . 2238 2248 + MwoI GCNNNNNNNGC 2244 2241 . . 2238 2241 + HhaI GCGC 2240 2238 . . 2273 2277 + Tsp4CI* ACNGT 2275 2274 . . 2283 2287 + BmgT120I GGNCC 2284 2285 . . 2283 2287 + AsuI* GGNCC 2283 2286 . . 2281 2284 - MnlI CCTC 2274 2273 . . 2284 2287 + CviJI RGCY 2285 2285 . . 2284 2287 + HaeIII GGCC 2285 2285 . . 2285 2289 - BceAI ACGGC 2270 2272 . . 2292 2297 - BtgZI GCGATG 2277 2281 . . 2297 2301 + BccI CCATC 2305 2306 . . 2300 2303 + TaqI TCGA 2300 2302 . . 2304 2307 + Csp6I GTAC 2304 2306 . . 2304 2307 + RsaI GTAC 2305 2305 . . 2311 2315 + SfaNI GCATC 2320 2324 . . 2314 2318 + Hpy188I TCNGA 2316 2315 . . 2317 2320 + DpnI GATC 2318 2318 . . 2317 2320 + BstKTI GATC 2319 2317 . . 2317 2320 + MboI GATC 2316 2320 . . 2333 2338 + Hpy178III* TCNNGA 2334 2336 . . 2341 2346 + SacI GAGCTC 2345 2341 . . 2341 2346 + Ecl136II GAGCTC 2343 2343 . . 2341 2346 + SduI GDGCHC 2345 2341 . . 2341 2346 + HgiJII* GRGCYC 2345 2341 . . 2341 2346 + HgiAI* GWGCWC 2345 2341 . . 2342 2345 + SetI ASST 2345 2341 . . 2342 2345 + CviJI RGCY 2343 2343 . . 2342 2345 + AluI AGCT 2343 2343 . . 2360 2364 + FauI CCCGC 2368 2370 . . 2361 2366 + NspBII* CMGCKG 2363 2363 . . 2361 2366 + SacII CCGCGG 2364 2362 . . 2361 2366 + DsaI* CCRYGG 2361 2365 . . 2361 2366 + SecI* CCNNGG 2361 2365 . . 2361 2371 + BsiYI* CCNNNNNNNGG 2367 2364 . . 2361 2364 + AciI CCGC 2361 2363 . . 2362 2365 + FnuDII* CGCG 2363 2363 . . 2363 2367 + TauI GCSGC 2366 2363 . . 2363 2367 + BlsI GCNGC 2365 2364 . . 2363 2367 + BisI GCNGC 2364 2365 . . 2365 2368 + CviJI RGCY 2366 2366 . . 2363 2366 - AciI CCGC 2363 2365 . . 2370 2373 + CviJI RGCY 2371 2371 . . 2370 2373 + HaeIII GGCC 2371 2371 . . 2370 2375 + NlaIV GGNNCC 2372 2372 . . 2370 2374 + BmgT120I GGNCC 2371 2372 . . 2370 2374 + AsuI* GGNCC 2370 2373 . . 2372 2377 + SecI* CCNNGG 2372 2376 . . 2373 2377 + BssKI CCNGG 2372 2377 . . 2373 2377 + ScrFI CCNGG 2374 2375 . . 2373 2377 + CauII* CCSGG 2374 2375 . . 2374 2377 + HpaII CCGG 2374 2376 . . 2379 2389 + MwoI GCNNNNNNNGC 2385 2382 . . 2376 2386 - BsaXI ACNNNNNCTCC 2398 2395 2368 2365 2387 2390 + CviRI* TGCA 2388 2388 . . 2404 2407 + SetI ASST 2407 2403 . . 2405 2408 + MnlI CCTC 2415 2414 . . 2433 2436 + AciI CCGC 2433 2435 . . 2433 2438 + BsrBI CCGCTC 2435 2435 . . 2436 2441 + SmlI CTYRAG 2436 2440 . . 2440 2445 + HaeII RGCGCY 2444 2440 . . 2441 2444 + Hin6I GCGC 2441 2443 . . 2441 2444 + GlaI GCGC 2442 2442 . . 2436 2441 - Bce83I* CTTGAG 2421 2419 . . 2441 2444 + HhaI GCGC 2443 2441 . . 2454 2458 - HgaI GACGC 2443 2448 . . 2459 2462 + CviJI RGCY 2460 2460 . . 2461 2464 + MnlI CCTC 2471 2470 . . 2463 2468 + Hpy178III* TCNNGA 2464 2466 . . 2464 2469 + SmlI CTYRAG 2464 2468 . . 2464 2469 + Bce83I* CTTGAG 2485 2483 . . 2467 2470 - MnlI CCTC 2460 2459 . . 2482 2487 + MfeI CAATTG 2482 2486 . . 2483 2486 + TspEI AATT 2482 2486 . . 2492 2495 + TspEI AATT 2491 2495 . . 2499 2504 + CfrI YGGCCR 2499 2503 . . 2500 2503 + CviJI RGCY 2501 2501 . . 2500 2503 + HaeIII GGCC 2501 2501 . . 2497 2502 - NmeAIII GCCGAG 2477 2475 . . 2508 2515 + SwaI ATTTAAAT 2511 2511 . . 2505 2509 - TspDTI ATGAA 2495 2493 . . 2509 2514 + AhaIII* TTTAAA 2511 2511 . . 2510 2513 + MseI TTAA 2510 2512 . . 2512 2517 + ApoI RAATTY 2512 2516 . . 2513 2516 + TspEI AATT 2512 2516 . . 2524 2527 + SetI ASST 2527 2523 . . 2525 2528 + MnlI CCTC 2535 2534 . . 2528 2531 + MnlI CCTC 2538 2537 . . 2526 2531 - BseRI GAGGAG 2517 2515 . . 2550 2559 + MslI CAYNNNNRTG 2554 2554 . . 2550 2559 + OliI CACNNNNGTG 2554 2554 . . 2554 2558 + Tsp4CI* ACNGT 2556 2555 . . 2555 2560 + DsaI* CCRYGG 2555 2559 . . 2555 2560 + SecI* CCNNGG 2555 2559 . . 2564 2569 + Hpy178III* TCNNGA 2565 2567 . . 2567 2572 + XhoII RGATCY 2567 2571 . . 2567 2572 + BglII AGATCT 2567 2571 . . 2563 2568 - Eco57MI CTGRAG 2548 2546 . . 2568 2571 + DpnI GATC 2569 2569 . . 2568 2571 + BstKTI GATC 2570 2568 . . 2563 2568 - GsuI CTGGAG 2548 2546 . . 2568 2571 + MboI GATC 2567 2571 . . 2573 2578 + NlaIV GGNNCC 2575 2575 . . 2573 2578 + HgiCI* GGYRCC 2573 2577 . . 2574 2579 + SduI GDGCHC 2578 2574 . . 2574 2579 + BseSI GKGCMC 2578 2574 . . 2579 2584 + MfeI CAATTG 2579 2583 . . 2580 2583 + TspEI AATT 2579 2583 . . 2584 2589 + BsmI GAATGC 2590 2588 . . 2587 2590 + CviRI* TGCA 2588 2588 . . 2590 2593 + SetI ASST 2593 2589 . . 2595 2600 + Hpy178III* TCNNGA 2596 2598 . . 2600 2603 + TspEI AATT 2599 2603 . . 2603 2606 + CviRI* TGCA 2604 2604 . . 2604 2614 + MwoI GCNNNNNNNGC 2610 2607 . . 2611 2616 + PvuII CAGCTG 2613 2613 . . 2611 2616 + NspBII* CMGCKG 2613 2613 . . 2612 2615 + SetI ASST 2615 2611 . . 2612 2615 + CviJI RGCY 2613 2613 . . 2612 2615 + AluI AGCT 2613 2613 . . 2613 2618 + Cac8I GCNNGC 2615 2615 . . 2616 2619 + CviJI RGCY 2617 2617 . . 2616 2619 + HaeIII GGCC 2617 2617 . . 2616 2620 + BmgT120I GGNCC 2617 2618 . . 2616 2620 + AsuI* GGNCC 2616 2619 . . 2619 2623 + BccI CCATC 2627 2628 . . 2619 2629 + BsiYI* CCNNNNNNNGG 2625 2622 . . 2622 2626 + Hpy188I TCNGA 2624 2623 . . 2620 2624 - FokI GGATG 2606 2610 . . 2620 2624 - BseGI GGATG 2619 2617 . . 2627 2630 + SetI ASST 2630 2626 . . 2625 2629 - Hin4II* CCTTC 2619 2618 . . 2631 2634 + CviRI* TGCA 2632 2632 . . 2632 2636 + BlsI GCNGC 2634 2633 . . 2632 2636 + BisI GCNGC 2633 2634 . . 2632 2636 + BbvI GCAGC 2644 2648 . . 2632 2636 + TseI GCWGC 2632 2635 . . 2633 2638 + NspBII* CMGCKG 2635 2635 . . 2635 2639 + TauI GCSGC 2638 2635 . . 2635 2639 + BlsI GCNGC 2637 2636 . . 2635 2639 + BisI GCNGC 2636 2637 . . 2637 2640 + CviJI RGCY 2638 2638 . . 2637 2642 + NlaIV GGNNCC 2639 2639 . . 2635 2638 - AciI CCGC 2635 2637 . . 2640 2645 + Hpy178III* TCNNGA 2641 2643 . . 2641 2651 + EcoNI CCTNNNNNAGG 2645 2646 . . 2641 2651 + BsiYI* CCNNNNNNNGG 2647 2644 . . 2644 2648 + TfiI GAWTC 2644 2647 . . 2644 2648 + HinfI GANTC 2644 2647 . . 2652 2656 + PleI GAGTC 2660 2661 . . 2652 2656 + MlyI GAGTC 2661 2661 . . 2652 2656 + HinfI GANTC 2652 2655 . . 2655 2660 + Hpy178III* TCNNGA 2656 2658 . . 2662 2667 + MstI* TGCGCA 2664 2664 . . 2663 2666 + Hin6I GCGC 2663 2665 . . 2663 2666 + GlaI GCGC 2664 2664 . . 2663 2666 + HhaI GCGC 2665 2663 . . 2665 2675 + MwoI GCNNNNNNNGC 2671 2668 . . 2665 2675 + BstAPI GCANNNNNTGC 2671 2668 . . 2667 2670 + TspEI AATT 2666 2670 . . 2668 2673 + VspI ATTAAT 2669 2671 . . 2669 2672 + MseI TTAA 2669 2671 . . 2677 2681 + Tsp4CI* ACNGT 2679 2678 . . 2686 2689 + MnlI CCTC 2696 2695 . . 2701 2705 + TauI GCSGC 2704 2701 . . 2701 2705 + BlsI GCNGC 2703 2702 . . 2701 2705 + BisI GCNGC 2702 2703 . . 2702 2705 + AciI CCGC 2702 2704 . . 2703 2706 + FnuDII* CGCG 2704 2704 . . 2704 2707 + Hin6I GCGC 2704 2706 . . 2704 2707 + GlaI GCGC 2705 2705 . . 2704 2707 + HhaI GCGC 2706 2704 . . 2713 2718 + AcyI GRCGYC 2714 2716 . . 2716 2720 + Tsp45I GTSAC 2715 2720 . . 2716 2720 + MaeIII GTNAC 2715 2720 . . 2714 2718 - HgaI GACGC 2703 2708 . . 2719 2723 + Tsp4CI* ACNGT 2721 2720 . . 2717 2721 - HphI GGTGA 2709 2708 . . 2731 2736 + BseRI GAGGAG 2746 2744 . . 2731 2734 - MnlI CCTC 2724 2723 . . 2734 2737 - MnlI CCTC 2727 2726 . . 2749 2753 + HgaI GACGC 2758 2763 . . 2749 2754 + AcyI GRCGYC 2750 2752 . . 2753 2756 + HpaII CCGG 2753 2755 . . 2755 2760 + NlaIV GGNNCC 2757 2757 . . 2757 2760 + CviJI RGCY 2758 2758 . . 2758 2762 - BceAI ACGGC 2743 2745 . . 2767 2770 + SetI ASST 2770 2766 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 6 1341 1428 # # AciI: # [CCGC] # 2 67 70 73 91 106 # 125 202 224 337 347 442 # 689 # # AclI: # [AACGTT] # 1019 1756 # # AcyI: # [GRCGYC] # 25 36 254 1211 1249 # # AflIII: # [ACRYGT] # 604 1013 1158 # # AhaIII*: # [TTTAAA] # 264 603 671 1237 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 1309 1432 # # AluI: # [AGCT] # 56 60 67 87 97 113 # 128 162 206 270 332 367 # 380 450 # # ApoI: # [RAATTY] # 150 197 263 574 604 987 # # AsuI*: # [GGNCC] # 87 159 246 366 720 1197 # # AsuII: # [TTCGAA] # 279 745 1751 # # AvaI: # [CYCGRG] # 57 2718 # # AvaII: # [GGWCC] # 720 858 1197 # # BaeI: # [ACNNNNGTAYC] # 33 926 1816 # # BamHI: # [GGATCC] # 1349 1426 # # BbvI: # [GCAGC] # 74 131 197 405 506 695 # 767 # # BccI: # [CCATC] # 148 210 322 486 567 1042 # # Bce83I*: # [CTTGAG] # 64 290 2421 # # BceAI: # [ACGGC] # 27 32 42 374 473 1827 # # BcgI: # [CGANNNNNNTGC] # 34 34 595 903 1209 # # BciVI: # [GTATCC] # 722 2053 # # BetI*: # [WCCGGW] # 736 970 1069 # # BglII: # [AGATCT] # 208 767 1800 # # BinI*: # [GGATC] # 13 111 265 509 570 1307 # # BisI: # [GCNGC] # 3 3 66 73 74 123 # 194 269 405 426 495 644 # # BlsI: # [GCNGC] # 3 3 66 72 74 123 # 194 269 405 426 496 644 # # BmeT110I: # [CYCGRG] # 58 2717 # # BmgT120I: # [GGNCC] # 87 158 246 366 720 1198 # # BsaBI: # [GATNNNNATC] # 1231 1544 # # BsaXI: # [ACNNNNNCTCC] # 30 30 177 377 2161 # # BseBI: # [CCWGG] # 7 174 1201 1393 # # BseGI: # [GGATG] # 156 325 583 588 1123 # # BseMII: # [CTCAG] # 2 11 292 321 698 700 # 751 # # BseRI: # [GAGGAG] # 29 197 229 306 667 1347 # # BseSI: # [GKGCMC] # 197 2578 # # BseYI: # [CCCAGC] # 82 2693 # # BsgI: # [GTGCAG] # 1313 1462 # # BsiYI*: # [CCNNNNNNNGG] # 22 128 192 218 258 313 # 663 981 # # BslFI: # [GGGAC] # 181 201 350 872 1171 # # BsmAI: # [GTCTC] # 44 65 578 906 1182 # # BsmI: # [GAATGC] # 27 185 246 421 501 1395 # # Bsp1407I: # [TGTACA] # 138 901 1736 # # BspCNI: # [CTCAG] # 4 12 294 319 697 700 # 749 # # BspMI: # [ACCTGC] # 6 183 1158 1428 # # BspMII*: # [TCCGGA] # 970 1805 # # BsrBI: # [CCGCTC] # 340 2435 # # BsrI: # [ACTGG] # 192 212 912 1459 # # BssKI: # [CCNGG] # 7 174 253 319 403 627 # 992 # # BstAPI: # [GCANNNNNTGC] # 104 2671 # # BstKTI: # [GATC] # 15 26 56 97 124 189 # 205 251 327 386 516 583 # # BstXI: # [CCANNNNNNTGG] # 953 1822 # # BtgZI: # [GCGATG] # 94 351 498 1832 # # BtrI: # [CACGTC] # 603 2172 # # Cac8I: # [GCNNGC] # 6 18 50 131 160 386 # 499 647 878 # # CauII*: # [CCSGG] # 255 401 627 1492 # # Cfr10I: # [RCCGGY] # 861 1914 # # CfrI: # [YGGCCR] # 276 687 1812 # # ClaI: # [ATCGAT] # 1303 1472 # # Csp6I: # [GTAC] # 138 162 267 471 794 943 # # CviAII: # [CATG] # 16 153 275 373 477 668 # 813 # # CviJI: # [RGCY] # 4 5 8 10 11 14 # 17 18 20 21 21 23 # 24 27 38 40 41 41 # 58 58 59 60 78 80 # 84 87 89 90 97 100 # 103 112 120 128 135 223 # 300 331 # # CviRI*: # [TGCA] # 16 28 72 105 143 200 # 221 223 320 723 724 # # DdeI: # [CTNAG] # 5 9 11 24 25 83 # 291 298 645 689 695 # # DpnI: # [GATC] # 15 26 56 97 124 188 # 206 251 327 386 516 583 # # DraII: # [RGGNCCY] # 1197 1578 # # DsaI*: # [CCRYGG] # 194 220 2361 # # Ecl136II: # [GAGCTC] # 56 432 1083 1204 # # Eco31I: # [GGTCTC] # 1226 1549 # # Eco57I: # [CTGAAG] # 241 2534 # # Eco57MI: # [CTGRAG] # 227 241 2307 # # EcoNI: # [CCTNNNNNAGG] # 130 2645 # # EcoRI: # [GAATTC] # 754 771 1250 # # EcoRII: # [CCWGG] # 7 174 1199 1395 # # EcoRV: # [GATATC] # 28 358 555 740 1094 # # EcoT22I: # [ATGCAT] # 830 1945 # # Esp3I: # [CGTCTC] # 578 906 1291 # # FatI: # [CATG] # 16 153 275 373 477 669 # 812 # # FauI: # [CCCGC] # 125 315 407 1928 # # FnuDII*: # [CGCG] # 71 87 125 158 176 341 # 802 1015 # # FokI: # [GGATG] # 169 312 583 608 1103 # # GlaI: # [GCGC] # 6 18 41 70 116 203 # 208 222 336 449 513 593 # # GsaI: # [CCCAGC] # 86 2689 # # GsuI: # [CTGGAG] # 227 2548 # # HaeII: # [RGCGCY] # 118 331 2326 # # HaeIII: # [GGCC] # 86 116 130 158 471 1814 # # HgaI: # [GACGC] # 17 55 196 221 254 260 # 783 989 # # HgiAI*: # [GWGCWC] # 58 430 1083 1204 # # HgiCI*: # [GGYRCC] # 202 243 1127 1203 # # HgiJII*: # [GRGCYC] # 58 183 430 1021 1083 # # HhaI: # [GCGC] # 6 18 41 69 117 203 # 208 222 336 449 513 593 # # Hin4I: # [GAYNNNNNVTC] # 0 0 12 32 32 32 # 32 32 46 223 582 850 # 902 # # Hin4II*: # [CCTTC] # 67 156 229 429 1894 # # Hin6I: # [GCGC] # 6 18 41 71 115 203 # 208 222 336 449 513 593 # # HindIII: # [AAGCTT] # 386 605 1784 # # HinfI: # [GANTC] # 6 6 8 108 111 123 # 124 140 143 210 221 246 # 291 302 310 426 # # HpaII: # [CCGG] # 22 90 125 255 334 379 # 626 944 # # HphI: # [GGTGA] # 66 346 595 1768 # # Hpy166II: # [GTNNAC] # 580 2195 # # Hpy178III*: # [TCNNGA] # 15 31 45 84 101 119 # 130 255 265 319 652 759 # # Hpy188I: # [TCNGA] # 12 72 151 152 198 243 # 289 308 333 475 542 # # Hpy99I: # [CGWCG] # 97 125 1242 1311 # # Ksp632I*: # [CTCTTC] # 8 288 748 760 971 # # MaeI: # [CTAG] # 4 157 333 524 567 1190 # # MaeII: # [ACGT] # 415 604 1756 # # MaeIII: # [GTNAC] # 60 914 1801 # # MboI: # [GATC] # 15 26 56 97 124 186 # 208 251 327 386 516 583 # # MboII: # [GAAGA] # 25 154 162 186 258 338 # 447 577 628 # # McrI*: # [CGRYCG] # 960 1815 # # MfeI: # [CAATTG] # 97 196 1215 1267 # # MluI: # [ACGCGT] # 1013 1762 # # MlyI: # [GAGTC] # 114 221 310 449 456 1225 # # MmeI: # [TCCRAC] # 425 2350 # # MnlI: # [CCTC] # 3 3 3 5 9 10 # 11 28 30 30 32 35 # 38 44 45 45 45 48 # 51 55 64 64 80 89 # 97 106 141 158 184 246 # 390 586 # # MseI: # [TTAA] # 34 81 90 96 106 121 # 159 357 494 541 696 # # MslI: # [CAYNNNNRTG] # 81 221 527 962 984 # # MstI*: # [TGCGCA] # 111 1210 1454 # # MwoI: # [GCNNNNNNNGC] # 7 61 83 104 141 225 # 317 443 691 703 # # NlaIII: # [CATG] # 16 153 275 373 477 665 # 816 # # NlaIV: # [GGNNCC] # 18 64 97 118 146 203 # 453 471 1205 # # NmeAIII: # [GCCGAG] # 274 298 2203 # # NruI: # [TCGCGA] # 695 2080 # # NspBII*: # [CMGCKG] # 22 140 250 2363 # # NspI: # [RCATGY] # 1191 1584 # # OliI: # [CACNNNNGTG] # 221 2554 # # PleI: # [GAGTC] # 115 221 310 448 456 1225 # # PpiI: # [GAACNNNNNCTC] # 32 1003 1740 # # PpuMI: # [RGGWCCY] # 1197 1578 # # PspXI: # [VCTCGAGB] # 57 2718 # # PvuII: # [CAGCTG] # 162 2613 # # RsaI: # [GTAC] # 138 162 267 470 794 944 # # SacI: # [GAGCTC] # 58 430 1083 1204 # # SacII: # [CCGCGG] # 411 2364 # # SapI: # [GCTCTTC] # 8 1259 1508 # # ScrFI: # [CCNGG] # 7 174 255 319 401 627 # 992 # # SduI: # [GDGCHC] # 58 183 197 233 1021 1083 # # SecI*: # [CCNNGG] # 11 139 183 220 842 1380 # # SetI: # [ASST] # 5 6 8 10 10 12 # 13 15 22 22 23 23 # 24 24 29 32 35 45 # 47 47 48 56 57 59 # 62 63 66 67 67 67 # 68 69 101 104 109 113 # 120 124 140 163 171 211 # 218 # # SfaNI: # [GCATC] # 334 371 455 1615 # # SfeI*: # [CTRYAG] # 971 1804 # # SmlI: # [CTYRAG] # 28 57 311 2379 # # SphI: # [GCATGC] # 1191 1584 # # SspI: # [AATATT] # 208 255 637 681 994 # # SwaI: # [ATTTAAAT] # 264 2511 # # TaiI: # [ACGT] # 415 601 1759 # # TaqI: # [TCGA] # 11 21 51 58 63 90 # 117 135 207 279 426 475 # 842 # # TaqII: # [CACCCA] # 636 2139 # # TatI: # [WGTACW] # 138 901 1736 # # TauI: # [GCSGC] # 66 71 272 549 691 1126 # # TfiI: # [GAWTC] # 6 108 111 124 131 140 # 143 308 310 636 758 # # TseI: # [GCWGC] # 74 143 197 405 494 695 # 767 # # Tsp45I: # [GTSAC] # 60 914 1801 # # Tsp4CI*: # [ACNGT] # 42 54 107 110 123 207 # 273 281 336 563 679 # # TspDTI: # [ATGAA] # 12 35 211 241 280 317 # 372 592 715 # # TspEI: # [AATT] # 9 15 20 21 23 39 # 40 59 59 62 67 67 # 91 106 109 113 115 138 # 143 197 197 222 396 467 # # TspGWI: # [ACGGA] # 143 1309 1323 # # TspRI: # [CASTGNN] # 812 1963 # # Tth111I: # [GACNNNGTC] # 1195 1580 # # VspI: # [ATTAAT] # 106 698 1971 # # XcmI: # [CCANNNNNNNNNTGG] # 1383 1392 # # XhoI: # [CTCGAG] # 57 2718 # # XhoII: # [RGATCY] # 208 451 509 767 840 # # XmaIII*: # [CGGCCG] # 963 1812 # # XmnI: # [GAANNNNTTC] # 104 708 889 1074 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2775 # Reported_sequences: 1 # Reported_hitcount: 764 #---------------------------------------