Restriction Fragments for HEM3/YDL205C



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The currently selected gene/sequence is :

HEM3/YDL205C on chromosome IV from coordinates 93745 to 92762.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25725.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 9 + DraII RGGNCCY 4 7 . . 3 8 + SduI GDGCHC 7 3 . . 3 8 + Bsp120I GGGCCC 3 7 . . 3 8 + NlaIV GGNNCC 5 5 . . 3 7 + BmgT120I GGNCC 4 5 . . 3 8 + HgiJII* GRGCYC 7 3 . . 3 8 + BseSI GKGCMC 7 3 . . 3 7 + AsuI* GGNCC 3 6 . . 3 8 + ApaI GGGCCC 7 3 . . 4 7 + CviJI RGCY 5 5 . . 4 7 + HaeIII GGCC 5 5 . . 4 8 + BmgT120I GGNCC 5 6 . . 4 8 + AsuI* GGNCC 4 7 . . 19 28 + MslI CAYNNNNRTG 23 23 . . 37 40 + TaqI TCGA 37 39 . . 41 44 + TspEI AATT 40 44 . . 46 49 - AciI CCGC 46 48 . . 58 63 + MmeI TCCRAC 83 81 . . 64 67 + FatI CATG 63 67 . . 64 67 + NlaIII CATG 67 63 . . 64 67 + CviAII CATG 64 66 . . 69 74 + AhaIII* TTTAAA 71 71 . . 70 73 + MseI TTAA 70 72 . . 78 81 + DpnI GATC 79 79 . . 78 81 + BstKTI GATC 80 78 . . 78 81 + MboI GATC 77 81 . . 80 83 + TaqI TCGA 80 82 . . 82 86 + MboII GAAGA 94 93 . . 90 95 + BciVI GTATCC 101 100 . . 93 98 + Hpy178III* TCNNGA 94 96 . . 93 98 + BetI* WCCGGW 93 97 . . 93 98 + BspMII* TCCGGA 93 97 . . 94 97 + HpaII CCGG 94 96 . . 106 109 + CviRI* TGCA 107 107 . . 110 113 + SetI ASST 113 109 . . 122 125 + CviRI* TGCA 123 123 . . 133 139 + BstEII GGTNACC 133 138 . . 133 137 + HphI GGTGA 145 144 . . 134 138 + Tsp45I GTSAC 133 138 . . 134 138 + MaeIII GTNAC 133 138 . . 135 146 + BdaI TGANNNNNNTCA 124 122 158 156 141 145 + TfiI GAWTC 141 144 . . 141 145 + HinfI GANTC 141 144 . . 146 149 + TspEI AATT 145 149 . . 135 146 - BdaI TGANNNNNNTCA 158 156 124 122 153 156 + SetI ASST 156 152 . . 158 163 + TatI WGTACW 158 162 . . 159 162 + Csp6I GTAC 159 161 . . 159 162 + RsaI GTAC 160 160 . . 170 173 - AciI CCGC 170 172 . . 176 181 + HindIII AAGCTT 176 180 . . 177 180 + SetI ASST 180 176 . . 177 180 + CviJI RGCY 178 178 . . 177 180 + AluI AGCT 178 178 . . 199 203 + MboII GAAGA 211 210 . . 199 204 + BbvII* GAAGAC 206 210 . . 195 200 - MmeI TCCRAC 176 174 . . 200 210 + FalI AAGNNNNNCTT 191 186 223 218 204 208 + BccI CCATC 212 213 . . 214 217 + FatI CATG 213 217 . . 214 217 + NlaIII CATG 217 213 . . 214 217 + CviAII CATG 214 216 . . 217 227 + Hin4I GAYNNNNNVTC 208 203 240 235 220 223 + DpnI GATC 221 221 . . 220 223 + BstKTI GATC 222 220 . . 220 223 + MboI GATC 219 223 . . 224 227 + MnlI CCTC 234 233 . . 220 224 - BinI* GGATC 214 215 . . 232 237 + HindIII AAGCTT 232 236 . . 232 242 + FalI AAGNNNNNCTT 223 218 255 250 233 236 + SetI ASST 236 232 . . 233 236 + CviJI RGCY 234 234 . . 233 236 + AluI AGCT 234 234 . . 232 242 - FalI AAGNNNNNCTT 255 250 223 218 243 246 + DpnI GATC 244 244 . . 243 246 + BstKTI GATC 245 243 . . 243 246 + MboI GATC 242 246 . . 248 252 - TspDTI ATGAA 238 236 . . 255 259 + Hpy188I TCNGA 257 256 . . 256 261 + Eco57MI CTGRAG 277 275 . . 256 261 + Eco57I CTGAAG 277 275 . . 258 262 - Hin4II* CCTTC 252 251 . . 263 268 + NspI RCATGY 267 263 . . 264 267 + FatI CATG 263 267 . . 264 267 + NlaIII CATG 267 263 . . 264 267 + CviAII CATG 264 266 . . 283 286 - MnlI CCTC 276 275 . . 290 293 + TaqI TCGA 290 292 . . 293 296 + SetI ASST 296 292 . . 293 296 + CviJI RGCY 294 294 . . 293 296 + AluI AGCT 294 294 . . 294 299 - GsaI CCCAGC 298 294 . . 294 299 - BseYI CCCAGC 294 298 . . 308 312 + DdeI CTNAG 308 311 . . 312 315 - AciI CCGC 312 314 . . 312 316 - FauI CCCGC 305 307 . . 317 320 + TaqI TCGA 317 319 . . 319 322 + DpnI GATC 320 320 . . 319 322 + BstKTI GATC 321 319 . . 319 322 + MboI GATC 318 322 . . 321 326 + MmeI TCCRAC 346 344 . . 319 323 - BinI* GGATC 313 314 . . 339 342 + FatI CATG 338 342 . . 339 342 + NlaIII CATG 342 338 . . 339 342 + CviAII CATG 339 341 . . 357 362 + PsiI TTATAA 359 359 . . 363 367 + BsmAI GTCTC 368 372 . . 366 371 + Hpy178III* TCNNGA 367 369 . . 369 373 + FokI GGATG 382 386 . . 369 373 + BseGI GGATG 375 373 . . 374 377 + SetI ASST 377 373 . . 375 379 + Hin4II* CCTTC 385 384 . . 378 383 + Hpy178III* TCNNGA 379 381 . . 379 389 + BsiYI* CCNNNNNNNGG 385 382 . . 397 402 + NlaIV GGNNCC 399 399 . . 400 403 + SetI ASST 403 399 . . 401 404 + MnlI CCTC 411 410 . . 404 408 - FokI GGATG 390 394 . . 404 408 - BseGI GGATG 403 401 . . 406 410 - TspGWI ACGGA 396 394 . . 413 417 + MboII GAAGA 425 424 . . 415 420 + XhoII RGATCY 415 419 . . 415 420 + BglII AGATCT 415 419 . . 416 419 + DpnI GATC 417 417 . . 416 419 + BstKTI GATC 418 416 . . 416 419 + MboI GATC 415 419 . . 421 427 + EspI* GCTNAGC 422 425 . . 422 426 + DdeI CTNAG 422 425 . . 422 426 + BspCNI CTCAG 435 433 . . 422 426 + BseMII CTCAG 436 434 . . 425 428 + SetI ASST 428 424 . . 425 428 + CviJI RGCY 426 426 . . 425 428 + AluI AGCT 426 426 . . 451 456 + ApoI RAATTY 451 455 . . 452 455 + TspEI AATT 451 455 . . 464 468 + Hpy188I TCNGA 466 465 . . 467 470 - MnlI CCTC 460 459 . . 482 485 + MaeI CTAG 482 484 . . 496 499 + MaeI CTAG 496 498 . . 502 512 + Hin4I GAYNNNNNVTC 493 488 525 520 502 512 - Hin4I GAYNNNNNVTC 525 520 493 488 516 519 + Csp6I GTAC 516 518 . . 516 519 + RsaI GTAC 517 517 . . 513 517 - TspGWI ACGGA 503 501 . . 522 527 + EcoT22I ATGCAT 526 522 . . 523 526 + CviRI* TGCA 524 524 . . 524 528 + SfaNI GCATC 533 537 . . 534 539 + AcyI GRCGYC 535 537 . . 535 539 - HgaI GACGC 524 529 . . 539 544 - EcoP15I CAGCAG 511 513 . . 541 546 - GsaI CCCAGC 545 541 . . 541 546 - BseYI CCCAGC 541 545 . . 549 553 - SfaNI GCATC 539 543 . . 561 566 - MmeI TCCRAC 542 540 . . 573 576 + TspEI AATT 572 576 . . 579 583 + BlsI GCNGC 581 580 . . 579 583 + BisI GCNGC 580 581 . . 579 583 + BbvI GCAGC 591 595 . . 579 583 + TseI GCWGC 579 582 . . 584 588 + TfiI GAWTC 584 587 . . 584 588 + HinfI GANTC 584 587 . . 592 596 + Hpy188I TCNGA 594 593 . . 594 599 - BciVI GTATCC 588 587 . . 603 606 + Csp6I GTAC 603 605 . . 603 606 + RsaI GTAC 604 604 . . 606 617 + BstXI CCANNNNNNTGG 613 609 . . 607 610 + FatI CATG 606 610 . . 607 610 + NlaIII CATG 610 606 . . 607 610 + CviAII CATG 607 609 . . 609 612 + CviRI* TGCA 610 610 . . 613 618 - MmeI TCCRAC 594 592 . . 622 627 + NarI GGCGCC 623 625 . . 622 627 + KasI GGCGCC 622 626 . . 622 627 + DinI GGCGCC 624 624 . . 622 627 + HaeII RGCGCY 626 622 . . 622 627 + NlaIV GGNNCC 624 624 . . 622 627 + AcyI GRCGYC 623 625 . . 622 627 + HgiCI* GGYRCC 622 626 . . 623 626 + Hin6I GCGC 623 625 . . 623 626 + GlaI GCGC 624 624 . . 623 626 + HhaI GCGC 625 623 . . 626 632 + PasI CCCWGGG 627 630 . . 626 631 + SecI* CCNNGG 626 630 . . 627 631 + BssKI CCNGG 626 631 . . 627 631 + BseBI CCWGG 628 629 . . 627 632 + SecI* CCNNGG 627 631 . . 627 631 + ScrFI CCNGG 628 629 . . 627 631 + EcoRII CCWGG 626 631 . . 639 642 + TspEI AATT 638 642 . . 649 653 + HphI GGTGA 661 660 . . 650 654 + Tsp45I GTSAC 649 654 . . 650 654 + MaeIII GTNAC 649 654 . . 654 663 + MslI CAYNNNNRTG 658 658 . . 664 668 + TspDTI ATGAA 679 677 . . 666 670 + MboII GAAGA 678 677 . . 669 673 + TfiI GAWTC 669 672 . . 669 673 + HinfI GANTC 669 672 . . 672 677 + Hpy178III* TCNNGA 673 675 . . 680 685 + ApoI RAATTY 680 684 . . 681 684 + TspEI AATT 680 684 . . 687 698 + BcgI CGANNNNNNTGC 676 674 710 708 688 691 + DpnI GATC 689 689 . . 688 691 + BstKTI GATC 690 688 . . 688 691 + MboI GATC 687 691 . . 696 699 + CviRI* TGCA 697 697 . . 715 719 + Hpy188I TCNGA 717 716 . . 720 725 + Cac8I GCNNGC 722 722 . . 742 745 - MnlI CCTC 735 734 . . 754 758 - TspGWI ACGGA 744 742 . . 772 776 + TfiI GAWTC 772 775 . . 772 776 + HinfI GANTC 772 775 . . 785 789 + TspDTI ATGAA 800 798 . . 787 791 + MboII GAAGA 799 798 . . 787 792 - Ksp632I* CTCTTC 782 785 . . 790 794 - BsmAI GTCTC 784 788 . . 800 803 + TspEI AATT 799 803 . . 808 811 + MseI TTAA 808 810 . . 813 816 + CviJI RGCY 814 814 . . 813 816 + HaeIII GGCC 814 814 . . 823 828 + Hpy166II GTNNAC 825 825 . . 823 828 + HindII GTYRAC 825 825 . . 827 830 + TaiI ACGT 830 826 . . 827 830 + SetI ASST 830 826 . . 827 830 + MaeII ACGT 827 829 . . 832 836 - Hin4II* CCTTC 826 825 . . 850 854 + MboII GAAGA 862 861 . . 850 855 + BbvII* GAAGAC 857 861 . . 858 861 + TspEI AATT 857 861 . . 881 884 + MseI TTAA 881 883 . . 886 890 + MboII GAAGA 898 897 . . 889 893 + TfiI GAWTC 889 892 . . 889 893 + HinfI GANTC 889 892 . . 893 898 + DsaI* CCRYGG 893 897 . . 893 898 + StyI CCWWGG 893 897 . . 893 898 + NcoI CCATGG 893 897 . . 893 898 + SecI* CCNNGG 893 897 . . 894 897 + FatI CATG 893 897 . . 894 897 + NlaIII CATG 897 893 . . 894 897 + CviAII CATG 894 896 . . 913 916 + MaeI CTAG 913 915 . . 915 918 + SetI ASST 918 914 . . 915 918 + CviJI RGCY 916 916 . . 915 918 + AluI AGCT 916 916 . . 931 941 + MwoI GCNNNNNNNGC 937 934 . . 938 941 + Hin6I GCGC 938 940 . . 938 941 + GlaI GCGC 939 939 . . 934 938 - BccI CCATC 928 929 . . 938 941 + HhaI GCGC 940 938 . . 947 952 + ApoI RAATTY 947 951 . . 948 951 + TspEI AATT 947 951 . . 951 956 + Hpy178III* TCNNGA 952 954 . . 954 958 + FokI GGATG 967 971 . . 954 958 + BseGI GGATG 960 958 . . 956 960 + TspDTI ATGAA 971 969 . . 960 963 + TspEI AATT 959 963 . . 961 966 + VspI ATTAAT 962 964 . . 962 965 + MseI TTAA 962 964 . . 964 967 + TspEI AATT 963 967 . . 974 978 + TfiI GAWTC 974 977 . . 974 978 + HinfI GANTC 974 977 . . #--------------------------------------- # # # AciI: # [CCGC] # 46 124 142 672 # # AcyI: # [GRCGYC] # 88 361 535 # # AhaIII*: # [TTTAAA] # 71 913 # # AluI: # [AGCT] # 56 60 68 132 178 490 # # ApaI: # [GGGCCC] # 7 977 # # ApoI: # [RAATTY] # 37 229 267 451 # # AsuI*: # [GGNCC] # 1 3 980 # # BbvI: # [GCAGC] # 393 591 # # BbvII*: # [GAAGAC] # 127 206 651 # # BccI: # [CCATC] # 56 212 716 # # BcgI: # [CGANNNNNNTGC] # 34 274 676 # # BciVI: # [GTATCC] # 101 396 487 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 124 826 # # BetI*: # [WCCGGW] # 93 891 # # BglII: # [AGATCT] # 415 569 # # BinI*: # [GGATC] # 99 214 671 # # BisI: # [GCNGC] # 404 580 # # BlsI: # [GCNGC] # 403 581 # # BmgT120I: # [GGNCC] # 1 4 979 # # BseBI: # [CCWGG] # 356 628 # # BseGI: # [GGATG] # 24 28 375 557 # # BseMII: # [CTCAG] # 436 548 # # BseSI: # [GKGCMC] # 7 977 # # BseYI: # [CCCAGC] # 247 294 443 # # BsiYI*: # [CCNNNNNNNGG] # 385 599 # # BsmAI: # [GTCTC] # 200 368 416 # # Bsp120I: # [GGGCCC] # 3 981 # # BspCNI: # [CTCAG] # 435 549 # # BspMII*: # [TCCGGA] # 93 891 # # BssKI: # [CCNGG] # 358 626 # # BstEII: # [GGTNACC] # 133 851 # # BstKTI: # [GATC] # 23 76 80 97 142 272 # 294 # # BstXI: # [CCANNNNNNTGG] # 371 613 # # Cac8I: # [GCNNGC] # 262 722 # # Csp6I: # [GTAC] # 87 159 357 381 # # CviAII: # [CATG] # 50 64 75 90 150 268 # 287 # # CviJI: # [RGCY] # 5 56 60 68 102 132 # 173 388 # # CviRI*: # [TGCA] # 16 86 87 107 287 401 # # DdeI: # [CTNAG] # 114 308 562 # # DinI: # [GGCGCC] # 360 624 # # DpnI: # [GATC] # 23 76 79 97 142 272 # 295 # # DraII: # [RGGNCCY] # 4 980 # # DsaI*: # [CCRYGG] # 91 893 # # Eco57I: # [CTGAAG] # 277 707 # # Eco57MI: # [CTGRAG] # 277 707 # # EcoP15I: # [CAGCAG] # 473 511 # # EcoRII: # [CCWGG] # 358 626 # # EcoT22I: # [ATGCAT] # 458 526 # # EspI*: # [GCTNAGC] # 422 562 # # FalI: # [AAGNNNNNCTT] # 0 0 0 32 32 191 # 729 # # FatI: # [CATG] # 50 63 75 91 150 268 # 287 # # FauI: # [CCCGC] # 305 679 # # FokI: # [GGATG] # 8 17 382 577 # # GlaI: # [GCGC] # 45 315 624 # # GsaI: # [CCCAGC] # 247 298 439 # # HaeII: # [RGCGCY] # 358 626 # # HaeIII: # [GGCC] # 5 170 809 # # HgaI: # [GACGC] # 460 524 # # HgiCI*: # [GGYRCC] # 362 622 # # HgiJII*: # [GRGCYC] # 7 977 # # HhaI: # [GCGC] # 44 315 625 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 208 253 # 459 # # Hin4II*: # [CCTTC] # 133 158 252 441 # # Hin6I: # [GCGC] # 46 315 623 # # HindII: # [GTYRAC] # 159 825 # # HindIII: # [AAGCTT] # 56 176 752 # # HinfI: # [GANTC] # 10 85 85 103 117 141 # 443 # # HpaII: # [CCGG] # 94 890 # # HphI: # [GGTGA] # 145 323 516 # # Hpy166II: # [GTNNAC] # 159 825 # # Hpy178III*: # [TCNNGA] # 12 32 94 273 279 294 # # Hpy188I: # [TCNGA] # 123 128 209 257 267 # # KasI: # [GGCGCC] # 362 622 # # Ksp632I*: # [CTCTTC] # 202 782 # # MaeI: # [CTAG] # 14 71 417 482 # # MaeII: # [ACGT] # 157 827 # # MaeIII: # [GTNAC] # 133 335 516 # # MboI: # [GATC] # 23 76 77 97 142 272 # 297 # # MboII: # [GAAGA] # 36 63 86 94 117 121 # 214 253 # # MmeI: # [TCCRAC] # 52 83 93 170 196 390 # # MnlI: # [CCTC] # 42 49 135 234 249 275 # # MseI: # [TTAA] # 22 70 73 81 738 # # MslI: # [CAYNNNNRTG] # 23 326 635 # # MwoI: # [GCNNNNNNNGC] # 47 937 # # NarI: # [GGCGCC] # 361 623 # # NcoI: # [CCATGG] # 91 893 # # NlaIII: # [CATG] # 50 67 75 87 150 268 # 287 # # NlaIV: # [GGNNCC] # 5 225 360 394 # # NspI: # [RCATGY] # 267 717 # # PasI: # [CCCWGGG] # 357 627 # # PsiI: # [TTATAA] # 359 625 # # RsaI: # [GTAC] # 87 160 357 380 # # ScrFI: # [CCNGG] # 356 628 # # SduI: # [GDGCHC] # 7 977 # # SecI*: # [CCNNGG] # 1 91 266 626 # # SetI: # [ASST] # 24 25 26 43 56 60 # 66 81 88 113 402 # # SfaNI: # [GCATC] # 6 445 533 # # StyI: # [CCWWGG] # 91 893 # # TaiI: # [ACGT] # 154 830 # # TaqI: # [TCGA] # 27 37 43 210 667 # # TatI: # [WGTACW] # 158 826 # # TfiI: # [GAWTC] # 10 85 85 103 117 141 # 443 # # TseI: # [GCWGC] # 405 579 # # Tsp45I: # [GTSAC] # 133 335 516 # # TspDTI: # [ATGAA] # 13 121 171 238 441 # # TspEI: # [AATT] # 4 12 21 40 42 58 # 66 90 105 119 121 306 # # TspGWI: # [ACGGA] # 107 240 241 396 # # VspI: # [ATTAAT] # 22 962 # # XhoII: # [RGATCY] # 415 569 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 984 # Reported_sequences: 1 # Reported_hitcount: 260 #---------------------------------------