Restriction Fragments for NUS1/YDL193W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

NUS1/YDL193W on chromosome IV from coordinates 114672 to 115799.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.10031.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 16 + BclI TGATCA 11 15 . . 12 15 + DpnI GATC 13 13 . . 12 15 + BstKTI GATC 14 12 . . 12 15 + MboI GATC 11 15 . . 21 25 + FokI GGATG 34 38 . . 21 25 + BseGI GGATG 27 25 . . 31 36 + BsrDI GCAATG 38 36 . . 36 41 + NlaIV GGNNCC 38 38 . . 37 42 + SduI GDGCHC 41 37 . . 37 42 + HgiJII* GRGCYC 41 37 . . 38 41 + CviJI RGCY 39 39 . . 47 51 + TspDTI ATGAA 62 60 . . 55 58 + AciI CCGC 55 57 . . 66 69 + DpnI GATC 67 67 . . 66 69 + BstKTI GATC 68 66 . . 66 69 + MboI GATC 65 69 . . 68 71 + TaqI TCGA 68 70 . . 84 89 + Hpy178III* TCNNGA 85 87 . . 74 85 - PpiI GAACNNNNNCTC 97 92 65 60 88 92 + TfiI GAWTC 88 91 . . 88 92 + HinfI GANTC 88 91 . . 91 95 - MboII GAAGA 83 82 . . 102 106 - FokI GGATG 88 92 . . 102 106 - BseGI GGATG 101 99 . . 108 111 + SetI ASST 111 107 . . 109 112 + MnlI CCTC 119 118 . . 111 116 + Hpy178III* TCNNGA 112 114 . . 112 122 + BsiYI* CCNNNNNNNGG 118 115 . . 112 122 + PflMI CCANNNNNTGG 118 115 . . 113 121 + AlwNI CAGNNNCTG 118 115 . . 115 119 + TfiI GAWTC 115 118 . . 115 119 + HinfI GANTC 115 118 . . 110 115 - Eco57MI CTGRAG 95 93 . . 110 115 - GsuI CTGGAG 95 93 . . 126 131 + AhaIII* TTTAAA 128 128 . . 127 130 + MseI TTAA 127 129 . . 134 137 - AciI CCGC 134 136 . . 143 146 + MseI TTAA 143 145 . . 145 148 + TspEI AATT 144 148 . . 159 162 + SetI ASST 162 158 . . 159 162 + CviJI RGCY 160 160 . . 159 162 + AluI AGCT 160 160 . . 160 170 + MwoI GCNNNNNNNGC 166 163 . . 162 167 + AhaIII* TTTAAA 164 164 . . 163 166 + MseI TTAA 163 165 . . 168 171 + CviJI RGCY 169 169 . . 168 171 + HaeIII GGCC 169 169 . . 172 176 + MaeIII GTNAC 171 176 . . 169 173 - BceAI ACGGC 154 156 . . 186 189 + CviRI* TGCA 187 187 . . 183 188 - BsrDI GCAATG 182 180 . . 190 194 + HgaI GACGC 199 204 . . 190 195 + AcyI GRCGYC 191 193 . . 198 209 + BdaI TGANNNNNNTCA 187 185 221 219 205 208 + MnlI CCTC 215 214 . . 206 210 + DdeI CTNAG 206 209 . . 206 210 + BspCNI CTCAG 219 217 . . 206 210 + BseMII CTCAG 220 218 . . 207 211 + Hpy188I TCNGA 209 208 . . 198 209 - BdaI TGANNNNNNTCA 221 219 187 185 210 214 + MboII GAAGA 222 221 . . 217 222 + Hpy166II GTNNAC 219 219 . . 226 230 - BsmAI GTCTC 220 224 . . 226 231 - Esp3I CGTCTC 220 224 . . 236 240 + PleI GAGTC 244 245 . . 236 240 + MlyI GAGTC 245 245 . . 236 240 + HinfI GANTC 236 239 . . 256 260 + MboII GAAGA 268 267 . . 276 279 + SetI ASST 279 275 . . 280 285 + ApoI RAATTY 280 284 . . 281 284 + TspEI AATT 280 284 . . 290 293 + SetI ASST 293 289 . . 345 348 + Csp6I GTAC 345 347 . . 345 348 + RsaI GTAC 346 346 . . 347 351 + Tsp4CI* ACNGT 349 348 . . 370 374 + TspDTI ATGAA 385 383 . . 376 380 + DdeI CTNAG 376 379 . . 381 386 + SspI AATATT 383 383 . . 403 406 + FatI CATG 402 406 . . 403 406 + NlaIII CATG 406 402 . . 403 406 + CviAII CATG 403 405 . . 404 409 + EcoT22I ATGCAT 408 404 . . 405 408 + CviRI* TGCA 406 406 . . 417 421 + Tsp4CI* ACNGT 419 418 . . 434 437 + TaiI ACGT 437 433 . . 434 437 + SetI ASST 437 433 . . 434 437 + MaeII ACGT 434 436 . . 442 445 + MnlI CCTC 452 451 . . 444 448 + Hpy188I TCNGA 446 445 . . 452 457 + ApoI RAATTY 452 456 . . 453 456 + TspEI AATT 452 456 . . 463 466 + SetI ASST 466 462 . . 467 472 + CfrI YGGCCR 467 471 . . 468 471 + CviJI RGCY 469 469 . . 468 471 + HaeIII GGCC 469 469 . . 469 473 + TauI GCSGC 472 469 . . 469 473 + BlsI GCNGC 471 470 . . 469 473 + BisI GCNGC 470 471 . . 470 473 + AciI CCGC 470 472 . . 488 491 + CviJI RGCY 489 489 . . 490 494 - BsrI ACTGG 490 488 . . 497 507 + MwoI GCNNNNNNNGC 503 500 . . 498 502 + Hpy99I CGWCG 502 497 . . 500 503 + TaiI ACGT 503 499 . . 500 503 + SetI ASST 503 499 . . 500 503 + MaeII ACGT 500 502 . . 506 510 + TauI GCSGC 509 506 . . 506 510 + BlsI GCNGC 508 507 . . 506 510 + BisI GCNGC 507 508 . . 506 509 - AciI CCGC 506 508 . . 509 512 - AciI CCGC 509 511 . . 514 518 + Tsp45I GTSAC 513 518 . . 514 518 + MaeIII GTNAC 513 518 . . 519 522 + SetI ASST 522 518 . . 526 529 + MseI TTAA 526 528 . . 532 536 + HgaI GACGC 541 546 . . 534 537 + FnuDII* CGCG 535 535 . . 543 546 + TspEI AATT 542 546 . . 556 560 + Tsp4CI* ACNGT 558 557 . . 563 568 + PvuII CAGCTG 565 565 . . 563 568 + NspBII* CMGCKG 565 565 . . 564 567 + SetI ASST 567 563 . . 564 567 + CviJI RGCY 565 565 . . 564 567 + AluI AGCT 565 565 . . 588 591 + Csp6I GTAC 588 590 . . 588 591 + RsaI GTAC 589 589 . . 598 602 - BccI CCATC 592 593 . . 603 608 + SspI AATATT 605 605 . . 621 626 + Hpy178III* TCNNGA 622 624 . . 626 629 + SetI ASST 629 625 . . 626 629 + CviJI RGCY 627 627 . . 626 629 + AluI AGCT 627 627 . . 628 632 + DdeI CTNAG 628 631 . . 628 632 - BspCNI CTCAG 620 618 . . 628 632 - BseMII CTCAG 619 617 . . 638 643 + ApoI RAATTY 638 642 . . 639 642 + TspEI AATT 638 642 . . 641 645 - TspDTI ATGAA 631 629 . . 646 651 + MmeI TCCRAC 671 669 . . 650 653 + SetI ASST 653 649 . . 651 655 + BssKI CCNGG 650 655 . . 651 655 + BseBI CCWGG 652 653 . . 651 655 + ScrFI CCNGG 652 653 . . 651 655 + EcoRII CCWGG 650 655 . . 654 657 + CviJI RGCY 655 655 . . 653 658 - TsoI TARCCA 643 641 . . 659 664 + SspI AATATT 661 661 . . 667 671 + BmgT120I GGNCC 668 669 . . 667 671 + AsuI* GGNCC 667 670 . . 668 671 + CviJI RGCY 669 669 . . 668 671 + HaeIII GGCC 669 669 . . 669 674 + Cac8I GCNNGC 671 671 . . 672 675 + SetI ASST 675 671 . . 672 675 + CviJI RGCY 673 673 . . 672 675 + AluI AGCT 673 673 . . 676 679 + FatI CATG 675 679 . . 676 679 + NlaIII CATG 679 675 . . 676 679 + CviAII CATG 676 678 . . 695 698 + MseI TTAA 695 697 . . 702 705 + SetI ASST 705 701 . . 703 706 + MnlI CCTC 713 712 . . 729 734 + XbaI TCTAGA 729 733 . . 729 734 + Hpy178III* TCNNGA 730 732 . . 730 733 + MaeI CTAG 730 732 . . 734 738 + TspGWI ACGGA 749 747 . . 738 741 + TspEI AATT 737 741 . . 748 752 - BsmAI GTCTC 742 746 . . 761 766 + BsrDI GCAATG 768 766 . . 774 777 + SetI ASST 777 773 . . 774 777 + CviJI RGCY 775 775 . . 774 777 + AluI AGCT 775 775 . . 777 782 + TsoI TARCCA 793 791 . . 780 790 + BsiYI* CCNNNNNNNGG 786 783 . . 795 798 + TspEI AATT 794 798 . . 806 811 + ApoI RAATTY 806 810 . . 807 810 + TspEI AATT 806 810 . . 829 833 - BccI CCATC 823 824 . . 843 853 + Hin4I GAYNNNNNVTC 834 829 866 861 848 851 + TaqI TCGA 848 850 . . 850 853 + DpnI GATC 851 851 . . 850 853 + BstKTI GATC 852 850 . . 850 853 + MboI GATC 849 853 . . 852 856 + Hpy188I TCNGA 854 853 . . 843 853 - Hin4I GAYNNNNNVTC 866 861 834 829 857 868 + BstXI CCANNNNNNTGG 864 860 . . 867 870 + CviJI RGCY 868 868 . . 869 873 + DdeI CTNAG 869 872 . . 869 873 - BspCNI CTCAG 861 859 . . 869 873 - BseMII CTCAG 860 858 . . 883 888 + Hpy166II GTNNAC 885 885 . . 883 888 + HpaI GTTAAC 885 885 . . 883 888 + HindII GTYRAC 885 885 . . 880 883 - AciI CCGC 880 882 . . 884 887 + MseI TTAA 884 886 . . 890 893 + TspEI AATT 889 893 . . 901 905 + Hpy188I TCNGA 903 902 . . 906 915 + MslI CAYNNNNRTG 910 910 . . 925 929 + TfiI GAWTC 925 928 . . 925 929 + HinfI GANTC 925 928 . . 928 932 + Hpy188I TCNGA 930 929 . . 942 947 + SpeI ACTAGT 942 946 . . 943 946 + MaeI CTAG 943 945 . . 949 954 + NlaIV GGNNCC 951 951 . . 949 953 + BmgT120I GGNCC 950 951 . . 949 953 + AvaII GGWCC 949 952 . . 949 953 + AsuI* GGNCC 949 952 . . 946 951 - MmeI TCCRAC 927 925 . . 955 967 + PsrI GAACNNNNNNTAC 947 942 979 974 966 970 + Tsp4CI* ACNGT 968 967 . . 979 983 + BmgT120I GGNCC 980 981 . . 979 983 + AsuI* GGNCC 979 982 . . 980 983 + CviJI RGCY 981 981 . . 980 983 + HaeIII GGCC 981 981 . . 982 986 + Hin4II* CCTTC 992 991 . . 987 992 - MmeI TCCRAC 968 966 . . 1001 1006 + NlaIV GGNNCC 1003 1003 . . 1008 1011 + SetI ASST 1011 1007 . . 1009 1014 + StyI CCWWGG 1009 1013 . . 1009 1014 + SecI* CCNNGG 1009 1013 . . 1024 1027 + MseI TTAA 1024 1026 . . 1030 1035 + ApoI RAATTY 1030 1034 . . 1031 1034 + TspEI AATT 1030 1034 . . 1075 1079 - TspDTI ATGAA 1065 1063 . . 1080 1085 + NspBII* CMGCKG 1082 1082 . . 1080 1085 + SacII CCGCGG 1083 1081 . . 1080 1085 + DsaI* CCRYGG 1080 1084 . . 1080 1085 + SecI* CCNNGG 1080 1084 . . 1080 1083 + AciI CCGC 1080 1082 . . 1077 1081 - FokI GGATG 1063 1067 . . 1077 1081 - BseGI GGATG 1076 1074 . . 1081 1084 + FnuDII* CGCG 1082 1082 . . 1082 1086 + TauI GCSGC 1085 1082 . . 1082 1086 + BlsI GCNGC 1084 1083 . . 1082 1086 + BisI GCNGC 1083 1084 . . 1084 1087 + CviJI RGCY 1085 1085 . . 1084 1087 + HaeIII GGCC 1085 1085 . . 1082 1085 - AciI CCGC 1082 1084 . . 1087 1091 + DdeI CTNAG 1087 1090 . . 1092 1096 + Tsp4CI* ACNGT 1094 1093 . . 1095 1098 + Csp6I GTAC 1095 1097 . . 1095 1098 + RsaI GTAC 1096 1096 . . 1102 1106 + FokI GGATG 1115 1119 . . 1102 1106 + BseGI GGATG 1108 1106 . . 1102 1113 - TstI CACNNNNNNTCC 1126 1121 1094 1089 #--------------------------------------- # # # AciI: # [CCGC] # 2 3 36 46 55 79 # 200 336 371 # # AcyI: # [GRCGYC] # 191 937 # # AhaIII*: # [TTTAAA] # 36 128 964 # # AluI: # [AGCT] # 46 62 102 160 353 405 # # AlwNI: # [CAGNNNCTG] # 118 1010 # # ApoI: # [RAATTY] # 98 168 172 186 224 280 # # AsuI*: # [GGNCC] # 30 149 282 667 # # AvaII: # [GGWCC] # 179 949 # # BccI: # [CCATC] # 231 305 592 # # BceAI: # [ACGGC] # 154 974 # # BclI: # [TGATCA] # 11 1117 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 187 907 # # BisI: # [GCNGC] # 37 45 470 576 # # BlsI: # [GCNGC] # 37 44 471 576 # # BmgT120I: # [GGNCC] # 30 148 282 668 # # BseBI: # [CCWGG] # 476 652 # # BseGI: # [GGATG] # 20 27 32 74 975 # # BseMII: # [CTCAG] # 220 241 268 399 # # BsiYI*: # [CCNNNNNNNGG] # 118 342 668 # # BsmAI: # [GTCTC] # 220 386 522 # # BspCNI: # [CTCAG] # 219 241 267 401 # # BsrDI: # [GCAATG] # 38 144 360 586 # # BsrI: # [ACTGG] # 490 638 # # BssKI: # [CCNGG] # 478 650 # # BstKTI: # [GATC] # 14 54 276 784 # # BstXI: # [CCANNNNNNTGG] # 264 864 # # Cac8I: # [GCNNGC] # 457 671 # # CfrI: # [YGGCCR] # 467 661 # # Csp6I: # [GTAC] # 33 243 345 507 # # CviAII: # [CATG] # 273 403 452 # # CviJI: # [RGCY] # 4 9 14 20 28 39 # 43 62 76 93 102 104 # 113 121 300 # # CviRI*: # [TGCA] # 187 219 722 # # DdeI: # [CTNAG] # 41 170 206 218 241 252 # # DpnI: # [GATC] # 13 54 277 784 # # DsaI*: # [CCRYGG] # 48 1080 # # Eco57MI: # [CTGRAG] # 95 1033 # # EcoRII: # [CCWGG] # 478 650 # # EcoT22I: # [ATGCAT] # 408 720 # # Esp3I: # [CGTCTC] # 220 908 # # FatI: # [CATG] # 273 402 453 # # FnuDII*: # [CGCG] # 46 535 547 # # FokI: # [GGATG] # 13 34 52 54 975 # # GsuI: # [CTGGAG] # 95 1033 # # HaeIII: # [GGCC] # 43 104 169 200 300 312 # # HgaI: # [GACGC] # 199 342 587 # # HgiJII*: # [GRGCYC] # 41 1087 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 262 834 # # Hin4II*: # [CCTTC] # 136 992 # # HindII: # [GTYRAC] # 243 885 # # HinfI: # [GANTC] # 27 88 121 203 689 # # HpaI: # [GTTAAC] # 243 885 # # Hpy166II: # [GTNNAC] # 219 243 666 # # Hpy178III*: # [TCNNGA] # 27 85 108 398 510 # # Hpy188I: # [TCNGA] # 27 49 198 209 237 408 # # Hpy99I: # [CGWCG] # 502 626 # # MaeI: # [CTAG] # 185 213 730 # # MaeII: # [ACGT] # 66 434 628 # # MaeIII: # [GTNAC] # 171 342 615 # # MboI: # [GATC] # 11 54 279 784 # # MboII: # [GAAGA] # 46 83 139 860 # # MlyI: # [GAGTC] # 245 883 # # MmeI: # [TCCRAC] # 41 160 256 671 # # MnlI: # [CCTC] # 96 119 237 261 415 # # MseI: # [TTAA] # 16 20 104 127 140 169 # 189 363 # # MslI: # [CAYNNNNRTG] # 218 910 # # MwoI: # [GCNNNNNNNGC] # 166 337 625 # # NlaIII: # [CATG] # 273 406 449 # # NlaIV: # [GGNNCC] # 38 52 125 913 # # NspBII*: # [CMGCKG] # 46 517 565 # # PflMI: # [CCANNNNNTGG] # 118 1010 # # PleI: # [GAGTC] # 244 884 # # PpiI: # [GAACNNNNNCTC] # 32 65 1031 # # PsrI: # [GAACNNNNNNTAC] # 32 149 947 # # PvuII: # [CAGCTG] # 563 565 # # RsaI: # [GTAC] # 32 243 346 507 # # SacII: # [CCGCGG] # 45 1083 # # ScrFI: # [CCNGG] # 476 652 # # SduI: # [GDGCHC] # 41 1087 # # SecI*: # [CCNNGG] # 48 71 1009 # # SetI: # [ASST] # 14 19 22 24 29 30 # 37 45 51 62 72 111 # 117 117 144 234 # # SpeI: # [ACTAGT] # 186 942 # # SspI: # [AATATT] # 56 222 383 467 # # StyI: # [CCWWGG] # 119 1009 # # TaiI: # [ACGT] # 66 437 625 # # TaqI: # [TCGA] # 68 280 780 # # TauI: # [GCSGC] # 37 43 472 576 # # TfiI: # [GAWTC] # 27 88 203 810 # # TsoI: # [TARCCA] # 150 335 643 # # Tsp45I: # [GTSAC] # 513 615 # # Tsp4CI*: # [ACNGT] # 34 70 126 139 349 410 # # TspDTI: # [ATGAA] # 62 63 246 323 434 # # TspEI: # [AATT] # 12 57 83 90 96 98 # 99 136 141 144 172 # # TspGWI: # [ACGGA] # 379 749 # # TstI: # [CACNNNNNNTCC] # 2 32 1094 # # XbaI: # [TCTAGA] # 399 729 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1128 # Reported_sequences: 1 # Reported_hitcount: 244 #---------------------------------------