Restriction Fragments for ENT1/YDL161W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ENT1/YDL161W on chromosome IV from coordinates 167714 to 169078.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.19606.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + TaqI TCGA 4 6 . . 11 14 + TspEI AATT 10 14 . . 19 24 + XhoII RGATCY 19 23 . . 19 24 + BglII AGATCT 19 23 . . 20 23 + DpnI GATC 21 21 . . 20 23 + BstKTI GATC 22 20 . . 20 23 + MboI GATC 19 23 . . 27 31 + MboII GAAGA 39 38 . . 30 35 + ApoI RAATTY 30 34 . . 31 34 + TspEI AATT 30 34 . . 36 40 + HphI GGTGA 48 47 . . 45 48 + Csp6I GTAC 45 47 . . 45 48 + RsaI GTAC 46 46 . . 48 53 + Ksp632I* CTCTTC 54 57 . . 49 53 - MboII GAAGA 41 40 . . 56 61 + SecI* CCNNGG 56 60 . . 57 61 + BssKI CCNGG 56 61 . . 57 61 + BseBI CCWGG 58 59 . . 57 61 + ScrFI CCNGG 58 59 . . 57 61 + EcoRII CCWGG 56 61 . . 57 67 + BsiYI* CCNNNNNNNGG 63 60 . . 57 67 + PflMI CCANNNNNTGG 63 60 . . 58 66 + AlwNI CAGNNNCTG 63 60 . . 59 62 + SetI ASST 62 58 . . 59 65 + PpuMI RGGWCCY 60 63 . . 59 65 + DraII RGGNCCY 60 63 . . 60 64 + BmgT120I GGNCC 61 62 . . 60 64 + AvaII GGWCC 60 63 . . 60 64 + AsuI* GGNCC 60 63 . . 63 67 + BssKI CCNGG 62 67 . . 63 67 + BseBI CCWGG 64 65 . . 63 67 + ScrFI CCNGG 64 65 . . 63 67 + EcoRII CCWGG 62 67 . . 75 78 + CviRI* TGCA 76 76 . . 79 82 + TaiI ACGT 82 78 . . 79 82 + SetI ASST 82 78 . . 79 82 + MaeII ACGT 79 81 . . 104 109 + StyI CCWWGG 104 108 . . 104 109 + SecI* CCNNGG 104 108 . . 109 113 + HinfI GANTC 109 112 . . 109 113 - PleI GAGTC 103 104 . . 109 113 - MlyI GAGTC 103 103 . . 115 118 + MseI TTAA 115 117 . . 117 120 + TspEI AATT 116 120 . . 122 125 + SetI ASST 125 121 . . 122 125 + CviJI RGCY 123 123 . . 122 125 + AluI AGCT 123 123 . . 123 128 + Cac8I GCNNGC 125 125 . . 126 129 + CviJI RGCY 127 127 . . 137 142 + NdeI CATATG 138 140 . . 146 149 + Hin6I GCGC 146 148 . . 146 149 + GlaI GCGC 147 147 . . 146 149 + HhaI GCGC 148 146 . . 157 162 + AsuII TTCGAA 158 160 . . 158 161 + TaqI TCGA 158 160 . . 155 159 - MboII GAAGA 147 146 . . 165 174 + MslI CAYNNNNRTG 169 169 . . 165 168 + FatI CATG 164 168 . . 165 168 + NlaIII CATG 168 164 . . 165 168 + CviAII CATG 165 167 . . 188 191 + MseI TTAA 188 190 . . 206 210 + BsrI ACTGG 211 209 . . 207 212 + Eco57MI CTGRAG 228 226 . . 207 212 + GsuI CTGGAG 228 226 . . 210 213 - MnlI CCTC 203 202 . . 232 236 + Tsp4CI* ACNGT 234 233 . . 248 253 + BclI TGATCA 248 252 . . 249 252 + DpnI GATC 250 250 . . 249 252 + BstKTI GATC 251 249 . . 249 252 + MboI GATC 248 252 . . 253 256 + SetI ASST 256 252 . . 268 271 + TspEI AATT 267 271 . . 291 296 + ApoI RAATTY 291 295 . . 292 295 + TspEI AATT 291 295 . . 305 310 + Hpy178III* TCNNGA 306 308 . . 314 325 + BdaI TGANNNNNNTCA 303 301 337 335 315 324 + XmnI GAANNNNTTC 319 319 . . 315 319 - Hin4II* CCTTC 309 308 . . 314 325 - BdaI TGANNNNNNTCA 337 335 303 301 328 333 + BsiI* CACGAG 328 332 . . 331 334 - MnlI CCTC 324 323 . . 340 343 - MnlI CCTC 333 332 . . 349 352 + DpnI GATC 350 350 . . 349 352 + BstKTI GATC 351 349 . . 349 352 + MboI GATC 348 352 . . 354 357 + SetI ASST 357 353 . . 383 386 + TspEI AATT 382 386 . . 390 393 + CviRI* TGCA 391 391 . . 400 404 + Hpy188I TCNGA 402 401 . . 421 424 - MnlI CCTC 414 413 . . 432 435 + FatI CATG 431 435 . . 432 435 + NlaIII CATG 435 431 . . 432 435 + CviAII CATG 432 434 . . 433 437 + TspDTI ATGAA 448 446 . . 446 450 + MboII GAAGA 458 457 . . 461 465 + MboII GAAGA 473 472 . . 464 468 + MboII GAAGA 476 475 . . 467 471 + MboII GAAGA 479 478 . . 470 474 - Hin4II* CCTTC 464 463 . . 478 482 + BsrI ACTGG 483 481 . . 478 483 + BfiI ACTGGG 488 487 . . 483 486 + Hin6I GCGC 483 485 . . 483 486 + GlaI GCGC 484 484 . . 483 486 + HhaI GCGC 485 483 . . 485 490 + BtsI GCAGTG 492 490 . . 486 492 + TspRI CASTGNN 492 483 . . 491 495 + TspDTI ATGAA 506 504 . . 509 512 + CviRI* TGCA 510 510 . . 516 519 + CviJI RGCY 517 517 . . 517 527 + MwoI GCNNNNNNNGC 523 520 . . 526 531 + Cac8I GCNNGC 528 528 . . 526 531 + BmtI GCTAGC 530 526 . . 526 531 + NheI GCTAGC 526 530 . . 527 530 + MaeI CTAG 527 529 . . 532 535 + SetI ASST 535 531 . . 534 539 + Hpy166II GTNNAC 536 536 . . 534 539 + HindII GTYRAC 536 536 . . 530 535 - BspMI ACCTGC 521 525 . . 538 542 + BceAI ACGGC 554 556 . . 540 545 + EciI GGCGGA 556 554 . . 544 548 + MboII GAAGA 556 555 . . 541 544 - AciI CCGC 541 543 . . 547 551 + MboII GAAGA 559 558 . . 547 552 + BbvII* GAAGAC 554 558 . . 557 561 + MboII GAAGA 569 568 . . 560 564 + MboII GAAGA 572 571 . . 574 577 - MnlI CCTC 567 566 . . 581 585 + TspDTI ATGAA 596 594 . . 588 591 + SetI ASST 591 587 . . 588 591 + CviJI RGCY 589 589 . . 588 591 + AluI AGCT 589 589 . . 593 596 + CviRI* TGCA 594 594 . . 607 611 + MboII GAAGA 619 618 . . 610 614 + MboII GAAGA 622 621 . . 613 618 + BseRI GAGGAG 628 626 . . 610 615 - Ksp632I* CTCTTC 605 608 . . 613 616 - MnlI CCTC 606 605 . . 617 620 + SetI ASST 620 616 . . 617 620 + CviJI RGCY 618 618 . . 617 620 + AluI AGCT 618 618 . . 629 632 + CviRI* TGCA 630 630 . . 644 649 + BsmI GAATGC 650 648 . . 647 650 + CviRI* TGCA 648 648 . . 648 658 + MwoI GCNNNNNNNGC 654 651 . . 661 664 + CviJI RGCY 662 662 . . 661 664 + HaeIII GGCC 662 662 . . 670 675 + MfeI CAATTG 670 674 . . 671 674 + TspEI AATT 670 674 . . 674 677 + CviRI* TGCA 675 675 . . 681 684 + SetI ASST 684 680 . . 703 709 + BstEII GGTNACC 703 708 . . 704 708 + MaeIII GTNAC 703 708 . . 717 722 + Hpy178III* TCNNGA 718 720 . . 718 724 + SauI* CCTNAGG 719 722 . . 719 723 + DdeI CTNAG 719 722 . . 719 723 - BspCNI CTCAG 711 709 . . 719 723 - BseMII CTCAG 710 708 . . 721 724 - MnlI CCTC 714 713 . . 734 737 + TspEI AATT 733 737 . . 739 744 + MfeI CAATTG 739 743 . . 740 743 + TspEI AATT 739 743 . . 743 746 + CviRI* TGCA 744 744 . . 744 754 + MwoI GCNNNNNNNGC 750 747 . . 753 763 + MwoI GCNNNNNNNGC 759 756 . . 771 775 + Tsp4CI* ACNGT 773 772 . . 776 779 + CviRI* TGCA 777 777 . . 791 796 + TatI WGTACW 791 795 . . 792 795 + Csp6I GTAC 792 794 . . 792 795 + RsaI GTAC 793 793 . . 806 811 + Hpy178III* TCNNGA 807 809 . . 810 814 + TfiI GAWTC 810 813 . . 810 814 + HinfI GANTC 810 813 . . 826 830 + MboII GAAGA 838 837 . . 832 835 + MaeI CTAG 832 834 . . 837 841 + Tsp4CI* ACNGT 839 838 . . 859 864 + SfeI* CTRYAG 859 863 . . 870 874 + Tsp4CI* ACNGT 872 871 . . 872 877 + TatI WGTACW 872 876 . . 872 877 + ScaI AGTACT 874 874 . . 873 876 + Csp6I GTAC 873 875 . . 873 876 + RsaI GTAC 874 874 . . 876 880 + DdeI CTNAG 876 879 . . 879 882 + SetI ASST 882 878 . . 879 882 + CviJI RGCY 880 880 . . 879 882 + AluI AGCT 880 880 . . 887 890 + CviRI* TGCA 888 888 . . 888 898 + MwoI GCNNNNNNNGC 894 891 . . 892 897 + EcoP15I CAGCAG 922 924 . . 894 898 + BlsI GCNGC 896 895 . . 894 898 + BisI GCNGC 895 896 . . 894 898 + BbvI GCAGC 906 910 . . 894 898 + TseI GCWGC 894 897 . . 904 909 + BsrDI GCAATG 911 909 . . 910 915 + MmeI TCCRAC 935 933 . . 931 935 + Hpy188I TCNGA 933 932 . . 939 944 + SacI GAGCTC 943 939 . . 939 944 + Ecl136II GAGCTC 941 941 . . 939 944 + SduI GDGCHC 943 939 . . 939 944 + HgiJII* GRGCYC 943 939 . . 939 944 + HgiAI* GWGCWC 943 939 . . 940 943 + SetI ASST 943 939 . . 940 943 + CviJI RGCY 941 941 . . 940 943 + AluI AGCT 941 941 . . 942 947 + SmlI CTYRAG 942 946 . . 947 952 + Hpy166II GTNNAC 949 949 . . 942 947 - Bce83I* CTTGAG 927 925 . . 939 950 - PpiI GAACNNNNNCTC 962 957 930 925 949 953 - HphI GGTGA 941 940 . . 957 961 + TspGWI ACGGA 972 970 . . 959 964 + NlaIV GGNNCC 961 961 . . 966 971 + SpeI ACTAGT 966 970 . . 967 970 + MaeI CTAG 967 969 . . 973 977 + BlsI GCNGC 975 974 . . 973 977 + BisI GCNGC 974 975 . . 973 977 + BbvI GCAGC 985 989 . . 973 977 + TseI GCWGC 973 976 . . 975 978 + SetI ASST 978 974 . . 975 978 + CviJI RGCY 976 976 . . 975 978 + AluI AGCT 976 976 . . 979 983 - MboII GAAGA 971 970 . . 988 996 + AlwNI CAGNNNCTG 993 990 . . 995 998 + CviRI* TGCA 996 996 . . 1016 1019 + CviJI RGCY 1017 1017 . . 1023 1027 + TfiI GAWTC 1023 1026 . . 1023 1027 + HinfI GANTC 1023 1026 . . 1035 1045 + Hin4I GAYNNNNNVTC 1026 1021 1058 1053 1035 1045 - Hin4I GAYNNNNNVTC 1058 1053 1026 1021 1067 1072 + Hpy166II GTNNAC 1069 1069 . . 1079 1082 + SetI ASST 1082 1078 . . 1079 1082 + CviJI RGCY 1080 1080 . . 1079 1082 + AluI AGCT 1080 1080 . . 1084 1087 + MaeI CTAG 1084 1086 . . 1085 1090 + TsoI TARCCA 1101 1099 . . 1086 1089 + CviJI RGCY 1087 1087 . . 1098 1101 + SetI ASST 1101 1097 . . 1112 1116 + Hpy188I TCNGA 1114 1113 . . 1128 1131 + CviJI RGCY 1129 1129 . . 1126 1129 - MnlI CCTC 1119 1118 . . 1152 1156 + MboII GAAGA 1164 1163 . . 1152 1157 + BbvII* GAAGAC 1159 1163 . . 1165 1169 - TspDTI ATGAA 1155 1153 . . 1171 1174 + TspEI AATT 1170 1174 . . 1175 1180 + SmlI CTYRAG 1175 1179 . . 1175 1180 - Bce83I* CTTGAG 1160 1158 . . 1201 1205 + Hpy188I TCNGA 1203 1202 . . 1203 1207 + FokI GGATG 1216 1220 . . 1203 1207 + BseGI GGATG 1209 1207 . . 1207 1210 + DpnI GATC 1208 1208 . . 1207 1210 + BstKTI GATC 1209 1207 . . 1207 1210 + MboI GATC 1206 1210 . . 1207 1211 - BinI* GGATC 1201 1202 . . 1221 1225 - TspGWI ACGGA 1211 1209 . . 1232 1236 + Tsp4CI* ACNGT 1234 1233 . . 1239 1244 + Hpy166II GTNNAC 1241 1241 . . 1239 1244 + BssNAI GTATAC 1241 1241 . . 1239 1244 + AccI GTMKAC 1240 1242 . . 1245 1248 + SetI ASST 1248 1244 . . 1247 1252 + Hpy166II GTNNAC 1249 1249 . . 1259 1262 + MaeI CTAG 1259 1261 . . 1263 1267 + Hpy99I CGWCG 1267 1262 . . 1262 1266 - HgaI GACGC 1251 1256 . . 1267 1270 + Csp6I GTAC 1267 1269 . . 1256 1267 - BcgI CGANNNNNNTGC 1279 1277 1245 1243 1267 1270 + RsaI GTAC 1268 1268 . . 1282 1285 + CviJI RGCY 1283 1283 . . 1288 1291 + CviJI RGCY 1289 1289 . . 1294 1300 + BstEII GGTNACC 1294 1299 . . 1295 1299 + MaeIII GTNAC 1294 1299 . . 1296 1301 + TsoI TARCCA 1312 1310 . . 1302 1306 + BsmAI GTCTC 1307 1311 . . 1299 1303 - BsrI ACTGG 1299 1297 . . 1309 1314 + EcoP15I CAGCAG 1339 1341 . . 1314 1318 + BsmAI GTCTC 1319 1323 . . 1327 1330 + CviJI RGCY 1328 1328 . . 1338 1342 + BssKI CCNGG 1337 1342 . . 1338 1341 + HpaII CCGG 1338 1340 . . 1338 1343 + SecI* CCNNGG 1338 1342 . . 1338 1342 + ScrFI CCNGG 1339 1340 . . 1338 1342 + CauII* CCSGG 1339 1340 . . 1353 1356 + TspEI AATT 1352 1356 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 125 1240 # # AciI: # [CCGC] # 541 824 # # AluI: # [AGCT] # 29 35 61 104 123 262 # 285 466 # # AlwNI: # [CAGNNNCTG] # 63 372 930 # # ApoI: # [RAATTY] # 30 261 1074 # # AsuI*: # [GGNCC] # 60 1305 # # AsuII: # [TTCGAA] # 158 1207 # # AvaII: # [GGWCC] # 60 1305 # # BbvI: # [GCAGC] # 79 380 906 # # BbvII*: # [GAAGAC] # 206 554 605 # # Bce83I*: # [CTTGAG] # 205 233 927 # # BceAI: # [ACGGC] # 554 811 # # BcgI: # [CGANNNNNNTGC] # 34 86 1245 # # BclI: # [TGATCA] # 248 1117 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 303 1028 # # BfiI: # [ACTGGG] # 488 877 # # BglII: # [AGATCT] # 19 1346 # # BinI*: # [GGATC] # 164 1201 # # BisI: # [GCNGC] # 79 391 895 # # BlsI: # [GCNGC] # 79 390 896 # # BmgT120I: # [GGNCC] # 61 1304 # # BmtI: # [GCTAGC] # 530 835 # # BseBI: # [CCWGG] # 6 58 1301 # # BseGI: # [GGATG] # 156 1209 # # BseMII: # [CTCAG] # 655 710 # # BseRI: # [GAGGAG] # 628 737 # # BsiI*: # [CACGAG] # 328 1037 # # BsiYI*: # [CCNNNNNNNGG] # 63 1302 # # BsmAI: # [GTCTC] # 12 46 1307 # # BsmI: # [GAATGC] # 650 715 # # BspCNI: # [CTCAG] # 654 711 # # BspMI: # [ACCTGC] # 521 844 # # BsrDI: # [GCAATG] # 454 911 # # BsrI: # [ACTGG] # 66 211 272 816 # # BssKI: # [CCNGG] # 6 28 56 1275 # # BssNAI: # [GTATAC] # 124 1241 # # BstEII: # [GGTNACC] # 71 591 703 # # BstKTI: # [GATC] # 22 100 156 229 858 # # BtsI: # [GCAGTG] # 492 873 # # Cac8I: # [GCNNGC] # 125 403 837 # # CauII*: # [CCSGG] # 26 1339 # # Csp6I: # [GTAC] # 45 81 98 394 747 # # CviAII: # [CATG] # 165 267 933 # # CviJI: # [RGCY] # 4 6 7 29 35 37 # 39 41 42 44 61 63 # 72 123 154 218 390 # # CviRI*: # [TGCA] # 18 27 33 36 69 76 # 84 108 111 119 315 369 # # DdeI: # [CTNAG] # 157 489 719 # # DpnI: # [GATC] # 21 100 157 229 858 # # DraII: # [RGGNCCY] # 60 1305 # # EciI: # [GGCGGA] # 556 809 # # Ecl136II: # [GAGCTC] # 424 941 # # Eco57MI: # [CTGRAG] # 228 1137 # # EcoP15I: # [CAGCAG] # 26 417 922 # # EcoRII: # [CCWGG] # 6 56 1303 # # FatI: # [CATG] # 164 267 934 # # FokI: # [GGATG] # 149 1216 # # GlaI: # [GCGC] # 147 337 881 # # GsuI: # [CTGGAG] # 228 1137 # # HaeIII: # [GGCC] # 662 703 # # HgaI: # [GACGC] # 114 1251 # # HgiAI*: # [GWGCWC] # 422 943 # # HgiJII*: # [GRGCYC] # 422 943 # # HhaI: # [GCGC] # 148 337 880 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 307 1026 # # Hin4II*: # [CCTTC] # 155 309 901 # # Hin6I: # [GCGC] # 146 337 882 # # HindII: # [GTYRAC] # 536 829 # # HinfI: # [GANTC] # 109 213 342 701 # # HpaII: # [CCGG] # 27 1338 # # HphI: # [GGTGA] # 48 424 893 # # Hpy166II: # [GTNNAC] # 8 116 120 172 413 536 # # Hpy178III*: # [TCNNGA] # 89 306 412 558 # # Hpy188I: # [TCNGA] # 89 162 181 402 531 # # Hpy99I: # [CGWCG] # 98 1267 # # Ksp632I*: # [CTCTTC] # 54 551 760 # # MaeI: # [CTAG] # 106 117 135 175 305 527 # # MaeII: # [ACGT] # 79 1286 # # MaeIII: # [GTNAC] # 71 591 703 # # MboI: # [GATC] # 19 100 159 229 858 # # MboII: # [GAAGA] # 2 3 3 3 3 3 # 10 15 39 47 77 106 # 133 193 201 216 311 # # MfeI: # [CAATTG] # 69 626 670 # # MlyI: # [GAGTC] # 103 1262 # # MmeI: # [TCCRAC] # 430 935 # # MnlI: # [CCTC] # 9 39 81 108 121 153 # 203 246 405 # # MseI: # [TTAA] # 73 115 1177 # # MslI: # [CAYNNNNRTG] # 169 1196 # # MwoI: # [GCNNNNNNNGC] # 9 96 131 135 471 523 # # NdeI: # [CATATG] # 138 1227 # # NheI: # [GCTAGC] # 526 839 # # NlaIII: # [CATG] # 168 267 930 # # NlaIV: # [GGNNCC] # 404 961 # # PflMI: # [CCANNNNNTGG] # 63 1302 # # PleI: # [GAGTC] # 103 1262 # # PpiI: # [GAACNNNNNCTC] # 32 403 930 # # PpuMI: # [RGGWCCY] # 60 1305 # # RsaI: # [GTAC] # 46 81 97 394 747 # # SacI: # [GAGCTC] # 422 943 # # SauI*: # [CCTNAGG] # 646 719 # # ScaI: # [AGTACT] # 491 874 # # ScrFI: # [CCNGG] # 6 26 58 1275 # # SduI: # [GDGCHC] # 422 943 # # SecI*: # [CCNNGG] # 27 48 56 1234 # # SetI: # [ASST] # 19 20 29 35 43 56 # 61 62 64 101 104 117 # 131 147 178 198 # # SfeI*: # [CTRYAG] # 506 859 # # SmlI: # [CTYRAG] # 190 233 942 # # SpeI: # [ACTAGT] # 399 966 # # StyI: # [CCWWGG] # 104 1261 # # TaiI: # [ACGT] # 82 1283 # # TaqI: # [TCGA] # 4 154 1207 # # TatI: # [WGTACW] # 81 493 791 # # TfiI: # [GAWTC] # 213 342 810 # # TseI: # [GCWGC] # 79 392 894 # # TsoI: # [TARCCA] # 53 211 1101 # # Tsp4CI*: # [ACNGT] # 33 66 131 234 362 539 # # TspDTI: # [ATGAA] # 58 90 210 448 559 # # TspEI: # [AATT] # 6 10 13 20 24 63 # 86 91 151 182 288 431 # # TspGWI: # [ACGGA] # 154 239 972 # # TspRI: # [CASTGNN] # 492 873 # # XhoII: # [RGATCY] # 19 1346 # # XmnI: # [GAANNNNTTC] # 319 1046 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1365 # Reported_sequences: 1 # Reported_hitcount: 280 #---------------------------------------