Restriction Fragments for KIN28/YDL108W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

KIN28/YDL108W on chromosome IV from coordinates 267698 to 268699.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.4864.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 17 22 + TatI WGTACW 17 21 . . 18 23 + Hpy166II GTNNAC 20 20 . . 18 21 + Csp6I GTAC 18 20 . . 18 21 + RsaI GTAC 19 19 . . 61 64 + MseI TTAA 61 63 . . 82 87 + AclI AACGTT 83 85 . . 83 86 + TaiI ACGT 86 82 . . 83 86 + SetI ASST 86 82 . . 83 86 + MaeII ACGT 83 85 . . 76 86 - BaeI ACNNNNGTAYC 101 96 68 63 89 94 + VspI ATTAAT 90 92 . . 90 93 + MseI TTAA 90 92 . . 95 105 + Hin4I GAYNNNNNVTC 86 81 118 113 98 102 + TfiI GAWTC 98 101 . . 98 102 + HinfI GANTC 98 101 . . 100 104 - TspDTI ATGAA 90 88 . . 95 105 - Hin4I GAYNNNNNVTC 118 113 86 81 102 107 - BtgZI GCGATG 87 91 . . 109 122 + AjuI GAANNNNNNNTTGG 101 96 133 128 121 125 + HphI GGTGA 133 132 . . 124 127 - MnlI CCTC 117 116 . . 128 131 + Csp6I GTAC 128 130 . . 128 131 + RsaI GTAC 129 129 . . 136 139 - AciI CCGC 136 138 . . 142 147 + Hpy166II GTNNAC 144 144 . . 155 160 + Hpy166II GTNNAC 157 157 . . 155 160 + HindII GTYRAC 157 157 . . 166 170 + BsrI ACTGG 171 169 . . 170 174 + MboII GAAGA 182 181 . . 185 188 + MseI TTAA 185 187 . . 192 195 + DpnI GATC 193 193 . . 192 195 + BstKTI GATC 194 192 . . 192 195 + MboI GATC 191 195 . . 202 206 + Hpy188I TCNGA 204 203 . . 200 204 - FokI GGATG 186 190 . . 200 204 - BseGI GGATG 199 197 . . 205 210 + ApoI RAATTY 205 209 . . 206 209 + TspEI AATT 205 209 . . 208 213 + AhaIII* TTTAAA 210 210 . . 209 212 + MseI TTAA 209 211 . . 214 218 - BccI CCATC 208 209 . . 231 234 + SetI ASST 234 230 . . 231 234 + CviJI RGCY 232 232 . . 231 234 + AluI AGCT 232 232 . . 236 240 - TspGWI ACGGA 226 224 . . 245 248 + MseI TTAA 245 247 . . 249 252 + Csp6I GTAC 249 251 . . 249 252 + RsaI GTAC 250 250 . . 251 254 + SetI ASST 254 250 . . 252 255 + MnlI CCTC 262 261 . . 263 266 + CviRI* TGCA 264 264 . . 264 268 + BlsI GCNGC 266 265 . . 264 268 + BisI GCNGC 265 266 . . 264 268 + BbvI GCAGC 276 280 . . 264 268 + TseI GCWGC 264 267 . . 267 271 + SfaNI GCATC 276 280 . . 270 274 + Hpy188I TCNGA 272 271 . . 268 272 - FokI GGATG 254 258 . . 268 272 - BseGI GGATG 267 265 . . 275 278 + TaiI ACGT 278 274 . . 275 278 + SetI ASST 278 274 . . 275 278 + MaeII ACGT 275 277 . . 294 303 + MslI CAYNNNNRTG 298 298 . . 303 306 + CviJI RGCY 304 304 . . 313 316 + TspEI AATT 312 316 . . 314 321 + SwaI ATTTAAAT 317 317 . . 315 320 + AhaIII* TTTAAA 317 317 . . 316 319 + MseI TTAA 316 318 . . 327 332 + Hpy178III* TCNNGA 328 330 . . 328 333 + Eco57MI CTGRAG 349 347 . . 328 333 + GsuI CTGGAG 349 347 . . 346 349 + DpnI GATC 347 347 . . 346 349 + BstKTI GATC 348 346 . . 346 349 + MboI GATC 345 349 . . 348 353 + XbaI TCTAGA 348 352 . . 348 353 + Hpy178III* TCNNGA 349 351 . . 349 352 + MaeI CTAG 349 351 . . 353 356 + SetI ASST 356 352 . . 352 355 - MnlI CCTC 345 344 . . 378 382 + Tsp4CI* ACNGT 380 379 . . 381 386 + Hpy166II GTNNAC 383 383 . . 389 394 + EcoP15I CAGCAG 419 421 . . 398 401 + MseI TTAA 398 400 . . 404 407 + FatI CATG 403 407 . . 404 407 + NlaIII CATG 407 403 . . 404 407 + CviAII CATG 404 406 . . 407 411 + FokI GGATG 420 424 . . 407 411 + BseGI GGATG 413 411 . . 408 412 - SfaNI GCATC 398 402 . . 423 426 - MnlI CCTC 416 415 . . 436 441 - BsrDI GCAATG 435 433 . . 447 452 + ApoI RAATTY 447 451 . . 448 451 + TspEI AATT 447 451 . . 451 455 - TspDTI ATGAA 441 439 . . 458 461 + CviRI* TGCA 459 459 . . 465 470 + XhoII RGATCY 465 469 . . 465 469 + BinI* GGATC 473 474 . . 466 469 + DpnI GATC 467 467 . . 466 469 + BstKTI GATC 468 466 . . 466 469 + MboI GATC 465 469 . . 468 472 + Hpy188I TCNGA 470 469 . . 481 484 + TspEI AATT 480 484 . . 495 498 + SetI ASST 498 494 . . 493 497 - HphI GGTGA 485 484 . . 501 506 + BalI TGGCCA 503 503 . . 501 506 + CfrI YGGCCR 501 505 . . 502 505 + CviJI RGCY 503 503 . . 502 505 + HaeIII GGCC 503 503 . . 499 503 - BccI CCATC 493 494 . . 524 529 - TaqII GACCGA 514 512 . . 529 532 + MaeI CTAG 529 531 . . 538 548 + MwoI GCNNNNNNNGC 544 541 . . 543 548 + Cfr10I RCCGGY 543 547 . . 544 547 + HpaII CCGG 544 546 . . 546 549 + CviJI RGCY 547 547 . . 546 549 + HaeIII GGCC 547 547 . . 546 551 + NlaIV GGNNCC 548 548 . . 546 550 + BmgT120I GGNCC 547 548 . . 546 550 + AsuI* GGNCC 546 549 . . 553 556 + FatI CATG 552 556 . . 553 556 + NlaIII CATG 556 552 . . 553 556 + CviAII CATG 553 555 . . 569 573 + MaeIII GTNAC 568 573 . . 572 575 + TaiI ACGT 575 571 . . 572 575 + SetI ASST 575 571 . . 572 575 + MaeII ACGT 572 574 . . 573 577 + Hpy99I CGWCG 577 572 . . 577 581 + MaeIII GTNAC 576 581 . . 584 588 - BccI CCATC 578 579 . . 594 599 + HaeII RGCGCY 598 594 . . 595 598 + Hin6I GCGC 595 597 . . 595 598 + GlaI GCGC 596 596 . . 595 598 + HhaI GCGC 597 595 . . 601 614 + AjuI GAANNNNNNNTTGG 593 588 625 620 602 605 + TspEI AATT 601 605 . . 615 618 + SetI ASST 618 614 . . 615 618 + CviJI RGCY 616 616 . . 615 618 + AluI AGCT 616 616 . . 633 636 + CviJI RGCY 634 634 . . 640 650 + Hin4I GAYNNNNNVTC 631 626 663 658 640 645 + EcoRV GATATC 642 642 . . 640 650 - Hin4I GAYNNNNNVTC 663 658 631 626 666 669 + FnuDII* CGCG 667 667 . . 667 670 - AciI CCGC 667 669 . . 671 674 + TspEI AATT 670 674 . . 672 677 + VspI ATTAAT 673 675 . . 673 676 + MseI TTAA 673 675 . . 679 683 + DdeI CTNAG 679 682 . . 681 691 + FalI AAGNNNNNCTT 672 667 704 699 678 684 - Bpu10I CCTNAGC 679 682 . . 687 690 + SetI ASST 690 686 . . 683 688 - BciVI GTATCC 677 676 . . 681 691 - FalI AAGNNNNNCTT 704 699 672 667 697 701 + BssKI CCNGG 696 701 . . 697 701 + BseBI CCWGG 698 699 . . 697 701 + ScrFI CCNGG 698 699 . . 697 701 + EcoRII CCWGG 696 701 . . 708 719 + BdaI TGANNNNNNTCA 697 695 731 729 709 718 + BsaBI GATNNNNATC 713 713 . . 713 716 + TaqI TCGA 713 715 . . 715 718 + DpnI GATC 716 716 . . 715 718 + BstKTI GATC 717 715 . . 715 718 + MboI GATC 714 718 . . 708 719 - BdaI TGANNNNNNTCA 731 729 697 695 727 731 + MaeIII GTNAC 726 731 . . 729 734 + AclI AACGTT 730 732 . . 730 733 + TaiI ACGT 733 729 . . 730 733 + SetI ASST 733 729 . . 730 733 + MaeII ACGT 730 732 . . 723 735 - AloI GAACNNNNNNTCC 747 742 715 710 736 742 + DraII RGGNCCY 737 740 . . 737 741 + BmgT120I GGNCC 738 739 . . 737 741 + AsuI* GGNCC 737 740 . . 738 741 + CviJI RGCY 739 739 . . 738 741 + HaeIII GGCC 739 739 . . 740 746 + SauI* CCTNAGG 741 744 . . 741 745 + DdeI CTNAG 741 744 . . 745 749 + BslFI GGGAC 759 763 . . 750 753 + SetI ASST 753 749 . . 752 757 + SfeI* CTRYAG 752 756 . . 767 770 + CviJI RGCY 768 768 . . 767 770 + HaeIII GGCC 768 768 . . 767 771 + BmgT120I GGNCC 768 769 . . 767 771 + AsuI* GGNCC 767 770 . . 778 782 - MboII GAAGA 770 769 . . 790 793 + TaiI ACGT 793 789 . . 790 793 + SetI ASST 793 789 . . 790 793 + MaeII ACGT 790 792 . . 801 805 + MaeIII GTNAC 800 805 . . 814 817 + AciI CCGC 814 816 . . 813 818 - EciI GGCGGA 803 801 . . 820 824 + Hin4II* CCTTC 830 829 . . 823 828 + Hpy178III* TCNNGA 824 826 . . 830 834 + TspDTI ATGAA 845 843 . . 833 836 + TspEI AATT 832 836 . . 837 840 - MnlI CCTC 830 829 . . 840 849 + XmnI GAANNNNTTC 844 844 . . 844 847 + SetI ASST 847 843 . . 847 851 - TspDTI ATGAA 837 835 . . 853 857 + BlsI GCNGC 855 854 . . 853 857 + BisI GCNGC 854 855 . . 853 857 + TseI GCWGC 853 856 . . 849 854 - BsrDI GCAATG 848 846 . . 856 861 + Cac8I GCNNGC 858 858 . . 856 861 + BmtI GCTAGC 860 856 . . 856 861 + NheI GCTAGC 856 860 . . 857 860 + MaeI CTAG 857 859 . . 853 857 - BbvI GCAGC 840 844 . . 870 874 + DdeI CTNAG 870 873 . . 887 892 + BsmI GAATGC 893 891 . . 898 902 + TspDTI ATGAA 913 911 . . 904 918 + XcmI CCANNNNNNNNNTGG 911 910 . . 907 918 + CspCI CAANNNNNGTGG 895 893 930 928 913 916 + SetI ASST 916 912 . . 912 915 - MnlI CCTC 905 904 . . 915 920 + Hpy166II GTNNAC 917 917 . . 917 921 + BmgT120I GGNCC 918 919 . . 917 921 + AvaII GGWCC 917 920 . . 917 921 + AsuI* GGNCC 917 920 . . 920 925 + NspBII* CMGCKG 922 922 . . 920 923 + AciI CCGC 920 922 . . 928 934 + TspRI CASTGNN 934 925 . . 956 959 + TspEI AATT 955 959 . . 965 973 + DraIII CACNNNGTG 970 967 . . 971 975 + Tsp45I GTSAC 970 975 . . 971 975 + MaeIII GTNAC 970 975 . . 979 983 - MboII GAAGA 971 970 . . 978 983 - BbvII* GAAGAC 971 975 . . 992 997 + SnaBI TACGTA 994 994 . . 992 997 + BsaAI YACGTR 994 994 . . 993 996 + TaiI ACGT 996 992 . . 993 996 + SetI ASST 996 992 . . 993 996 + MaeII ACGT 993 995 . . 995 999 + MaeIII GTNAC 994 999 . . #--------------------------------------- # # # AciI: # [CCGC] # 82 106 136 147 531 # # AclI: # [AACGTT] # 83 272 647 # # AhaIII*: # [TTTAAA] # 107 210 685 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 101 377 460 # # AloI: # [GAACNNNNNNTCC] # 32 255 715 # # AluI: # [AGCT] # 232 384 386 # # ApoI: # [RAATTY] # 205 242 555 # # AsuI*: # [GGNCC] # 30 85 150 191 546 # # AvaII: # [GGWCC] # 85 917 # # BaeI: # [ACNNNNGTAYC] # 33 68 901 # # BalI: # [TGGCCA] # 499 503 # # BbvI: # [GCAGC] # 162 276 564 # # BbvII*: # [GAAGAC] # 31 971 # # BccI: # [CCATC] # 85 208 285 424 # # BciVI: # [GTATCC] # 325 677 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 271 697 # # BinI*: # [GGATC] # 473 529 # # BisI: # [GCNGC] # 148 265 589 # # BlsI: # [GCNGC] # 147 266 589 # # BmgT120I: # [GGNCC] # 30 84 150 191 547 # # BmtI: # [GCTAGC] # 142 860 # # Bpu10I: # [CCTNAGC] # 323 679 # # BsaAI: # [YACGTR] # 8 994 # # BsaBI: # [GATNNNNATC] # 289 713 # # BseBI: # [CCWGG] # 304 698 # # BseGI: # [GGATG] # 68 146 199 589 # # BslFI: # [GGGAC] # 243 759 # # BsmI: # [GAATGC] # 109 893 # # BsrDI: # [GCAATG] # 154 413 435 # # BsrI: # [ACTGG] # 171 831 # # BssKI: # [CCNGG] # 306 696 # # BstKTI: # [GATC] # 120 154 194 249 285 # # BtgZI: # [GCGATG] # 87 915 # # Cac8I: # [GCNNGC] # 144 858 # # Cfr10I: # [RCCGGY] # 459 543 # # CfrI: # [YGGCCR] # 501 501 # # Csp6I: # [GTAC] # 18 110 121 753 # # CspCI: # [CAANNNNNGTGG] # 35 72 895 # # CviAII: # [CATG] # 149 404 449 # # CviJI: # [RGCY] # 18 29 44 69 72 105 # 199 232 234 # # CviRI*: # [TGCA] # 195 264 543 # # DdeI: # [CTNAG] # 62 129 132 679 # # DpnI: # [GATC] # 120 154 193 249 286 # # DraII: # [RGGNCCY] # 265 737 # # DraIII: # [CACNNNGTG] # 32 970 # # EciI: # [GGCGGA] # 199 803 # # Eco57MI: # [CTGRAG] # 349 653 # # EcoP15I: # [CAGCAG] # 419 583 # # EcoRII: # [CCWGG] # 306 696 # # EcoRV: # [GATATC] # 360 642 # # FalI: # [AAGNNNNNCTT] # 0 0 32 298 672 # # FatI: # [CATG] # 149 403 450 # # FnuDII*: # [CGCG] # 335 667 # # FokI: # [GGATG] # 68 166 186 582 # # GlaI: # [GCGC] # 406 596 # # GsuI: # [CTGGAG] # 349 653 # # HaeII: # [RGCGCY] # 404 598 # # HaeIII: # [GGCC] # 29 44 192 234 503 # # HhaI: # [GCGC] # 405 597 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 86 339 513 # # Hin4II*: # [CCTTC] # 172 830 # # Hin6I: # [GCGC] # 407 595 # # HindII: # [GTYRAC] # 157 845 # # HinfI: # [GANTC] # 98 904 # # HpaII: # [CCGG] # 458 544 # # HphI: # [GGTGA] # 133 352 517 # # Hpy166II: # [GTNNAC] # 13 20 85 124 226 534 # # Hpy178III*: # [TCNNGA] # 21 178 328 475 # # Hpy188I: # [TCNGA] # 68 198 204 532 # # Hpy99I: # [CGWCG] # 425 577 # # MaeI: # [CTAG] # 145 180 328 349 # # MaeII: # [ACGT] # 9 60 83 158 192 203 # 297 # # MaeIII: # [GTNAC] # 8 8 24 74 150 170 # 568 # # MboI: # [GATC] # 120 154 191 249 288 # # MboII: # [GAAGA] # 31 182 201 588 # # MnlI: # [CCTC] # 71 75 83 97 117 145 # 414 # # MseI: # [TTAA] # 24 29 36 61 71 82 # 95 275 329 # # MslI: # [CAYNNNNRTG] # 298 704 # # MwoI: # [GCNNNNNNNGC] # 458 544 # # NheI: # [GCTAGC] # 146 856 # # NlaIII: # [CATG] # 149 407 446 # # NlaIV: # [GGNNCC] # 454 548 # # NspBII*: # [CMGCKG] # 80 922 # # RsaI: # [GTAC] # 19 110 121 752 # # SauI*: # [CCTNAGG] # 261 741 # # ScrFI: # [CCNGG] # 304 698 # # SetI: # [ASST] # 6 20 20 24 40 43 # 43 54 69 72 77 78 # 80 86 142 148 # # SfaNI: # [GCATC] # 122 276 604 # # SfeI*: # [CTRYAG] # 250 752 # # SnaBI: # [TACGTA] # 8 994 # # SwaI: # [ATTTAAAT] # 317 685 # # TaiI: # [ACGT] # 6 60 86 158 192 203 # 297 # # TaqI: # [TCGA] # 289 713 # # TaqII: # [GACCGA] # 488 514 # # TatI: # [WGTACW] # 17 985 # # TfiI: # [GAWTC] # 98 904 # # TseI: # [GCWGC] # 149 264 589 # # Tsp45I: # [GTSAC] # 32 970 # # Tsp4CI*: # [ACNGT] # 380 622 # # TspDTI: # [ATGAA] # 8 16 68 74 89 351 # 396 # # TspEI: # [AATT] # 33 47 69 107 121 123 # 135 162 205 # # TspGWI: # [ACGGA] # 226 776 # # TspRI: # [CASTGNN] # 68 934 # # VspI: # [ATTAAT] # 90 329 583 # # XbaI: # [TCTAGA] # 348 654 # # XcmI: # [CCANNNNNNNNNTGG] # 91 911 # # XhoII: # [RGATCY] # 465 537 # # XmnI: # [GAANNNNTTC] # 158 844 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1002 # Reported_sequences: 1 # Reported_hitcount: 235 #---------------------------------------