Restriction Fragments for YDL094C



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YDL094C on chromosome IV from coordinates 290081 to 289572.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22720.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 23 26 + CviJI RGCY 24 24 . . 29 32 + MaeI CTAG 29 31 . . 32 41 + XmnI GAANNNNTTC 36 36 . . 33 38 + HindIII AAGCTT 33 37 . . 34 37 + SetI ASST 37 33 . . 34 37 + CviJI RGCY 35 35 . . 34 37 + AluI AGCT 35 35 . . 42 46 + DdeI CTNAG 42 45 . . 47 51 + TspDTI ATGAA 62 60 . . 52 56 + MaeIII GTNAC 51 56 . . 55 59 + Tsp4CI* ACNGT 57 56 . . 65 69 + TspDTI ATGAA 80 78 . . 74 77 + TspEI AATT 73 77 . . 104 107 + SetI ASST 107 103 . . 105 108 + MnlI CCTC 115 114 . . 112 116 + BmgT120I GGNCC 113 114 . . 112 116 + AsuI* GGNCC 112 115 . . 113 116 + CviJI RGCY 114 114 . . 113 116 + HaeIII GGCC 114 114 . . 114 118 + TauI GCSGC 117 114 . . 114 118 + BlsI GCNGC 116 115 . . 114 118 + BisI GCNGC 115 116 . . 115 118 + AciI CCGC 115 117 . . 116 119 + FnuDII* CGCG 117 117 . . 121 124 + MseI TTAA 121 123 . . 127 131 + Tsp45I GTSAC 126 131 . . 127 131 + MaeIII GTNAC 126 131 . . 137 141 + FokI GGATG 150 154 . . 137 141 + BseGI GGATG 143 141 . . 142 147 + XhoII RGATCY 142 146 . . 142 147 + NlaIV GGNNCC 144 144 . . 138 142 - BccI CCATC 132 133 . . 142 147 + BamHI GGATCC 142 146 . . 142 146 + BinI* GGATC 150 151 . . 143 146 + DpnI GATC 144 144 . . 143 146 + BstKTI GATC 145 143 . . 143 146 + MboI GATC 142 146 . . 143 147 - BinI* GGATC 137 138 . . 148 151 + SetI ASST 151 147 . . 149 153 + Hin4II* CCTTC 159 158 . . 150 155 - Eco57MI CTGRAG 135 133 . . 150 155 - Eco57I CTGAAG 135 133 . . 169 173 - TspDTI ATGAA 159 157 . . 175 180 + AhaIII* TTTAAA 177 177 . . 176 179 + MseI TTAA 176 178 . . 189 192 + DpnI GATC 190 190 . . 189 192 + BstKTI GATC 191 189 . . 189 192 + MboI GATC 188 192 . . 198 204 + AarI CACCTGC 208 212 . . 199 202 + SetI ASST 202 198 . . 199 204 + BspMI ACCTGC 208 212 . . 205 208 + MseI TTAA 205 207 . . 207 210 + TspEI AATT 206 210 . . 212 215 + MseI TTAA 212 214 . . 224 233 + XmnI GAANNNNTTC 228 228 . . 225 230 + SspI AATATT 227 227 . . 231 236 + AsuII TTCGAA 232 234 . . 232 235 + TaqI TCGA 232 234 . . 240 244 + MboII GAAGA 252 251 . . 259 264 + EcoT22I ATGCAT 263 259 . . 260 263 + CviRI* TGCA 261 261 . . 269 274 + TatI WGTACW 269 273 . . 270 273 + Csp6I GTAC 270 272 . . 270 273 + RsaI GTAC 271 271 . . 299 304 + Hpy178III* TCNNGA 300 302 . . 301 304 + TaqI TCGA 301 303 . . 303 308 + BsmI GAATGC 309 307 . . 313 316 + TaqI TCGA 313 315 . . 326 329 + FnuDII* CGCG 327 327 . . 327 331 + TauI GCSGC 330 327 . . 327 331 + BlsI GCNGC 329 328 . . 327 331 + BisI GCNGC 328 329 . . 327 330 - AciI CCGC 327 329 . . 331 336 + BsaAI YACGTR 333 333 . . 332 335 + TaiI ACGT 335 331 . . 332 335 + SetI ASST 335 331 . . 332 335 + MaeII ACGT 332 334 . . 334 339 + Hpy166II GTNNAC 336 336 . . 357 361 + Tsp4CI* ACNGT 359 358 . . 377 383 + TspRI CASTGNN 383 374 . . 390 393 + CviRI* TGCA 391 391 . . 394 397 + DpnI GATC 395 395 . . 394 397 + BstKTI GATC 396 394 . . 394 397 + MboI GATC 393 397 . . 421 426 + BsaAI YACGTR 423 423 . . 422 425 + TaiI ACGT 425 421 . . 422 425 + SetI ASST 425 421 . . 422 425 + MaeII ACGT 422 424 . . 422 427 + AflIII ACRYGT 422 426 . . 426 430 + BsmAI GTCTC 431 435 . . 439 443 - BccI CCATC 433 434 . . 443 454 + DrdI GACNNNNNNGTC 449 447 . . 455 458 + SetI ASST 458 454 . . 455 458 + CviJI RGCY 456 456 . . 455 458 + AluI AGCT 456 456 . . 459 464 + Hpy178III* TCNNGA 460 462 . . 477 482 + Hpy178III* TCNNGA 478 480 . . 477 482 + BspHI TCATGA 477 481 . . 478 481 + FatI CATG 477 481 . . 478 481 + NlaIII CATG 481 477 . . 478 481 + CviAII CATG 478 480 . . 476 480 - TspDTI ATGAA 466 464 . . 507 510 + MseI TTAA 507 509 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 208 302 # # AciI: # [CCGC] # 115 183 212 # # AflIII: # [ACRYGT] # 88 422 # # AhaIII*: # [TTTAAA] # 177 333 # # AluI: # [AGCT] # 35 54 421 # # AsuI*: # [GGNCC] # 112 398 # # AsuII: # [TTCGAA] # 232 278 # # BamHI: # [GGATCC] # 142 368 # # BccI: # [CCATC] # 77 132 301 # # BinI*: # [GGATC] # 13 137 360 # # BisI: # [GCNGC] # 115 182 213 # # BlsI: # [GCNGC] # 116 181 213 # # BmgT120I: # [GGNCC] # 113 397 # # BsaAI: # [YACGTR] # 87 90 333 # # BseGI: # [GGATG] # 143 367 # # BsmAI: # [GTCTC] # 79 431 # # BsmI: # [GAATGC] # 201 309 # # BspHI: # [TCATGA] # 33 477 # # BspMI: # [ACCTGC] # 208 302 # # BstKTI: # [GATC] # 46 114 145 205 # # Csp6I: # [GTAC] # 240 270 # # CviAII: # [CATG] # 32 478 # # CviJI: # [RGCY] # 11 24 54 79 342 # # CviRI*: # [TGCA] # 119 130 261 # # DdeI: # [CTNAG] # 42 468 # # DpnI: # [GATC] # 46 115 144 205 # # DrdI: # [GACNNNNNNGTC] # 61 449 # # Eco57I: # [CTGAAG] # 135 375 # # Eco57MI: # [CTGRAG] # 135 375 # # EcoT22I: # [ATGCAT] # 247 263 # # FatI: # [CATG] # 33 477 # # FnuDII*: # [CGCG] # 117 183 210 # # FokI: # [GGATG] # 150 360 # # HaeIII: # [GGCC] # 114 396 # # Hin4II*: # [CCTTC] # 159 351 # # HindIII: # [AAGCTT] # 33 477 # # Hpy166II: # [GTNNAC] # 174 336 # # Hpy178III*: # [TCNNGA] # 18 32 160 300 # # MaeI: # [CTAG] # 29 481 # # MaeII: # [ACGT] # 88 90 332 # # MaeIII: # [GTNAC] # 51 75 384 # # MboI: # [GATC] # 46 117 142 205 # # MboII: # [GAAGA] # 252 258 # # MnlI: # [CCTC] # 115 395 # # MseI: # [TTAA] # 3 7 29 55 121 295 # # NlaIII: # [CATG] # 29 481 # # NlaIV: # [GGNNCC] # 144 366 # # RsaI: # [GTAC] # 239 271 # # SetI: # [ASST] # 33 37 44 51 52 70 # 90 133 # # SspI: # [AATATT] # 227 283 # # TaiI: # [ACGT] # 85 90 335 # # TaqI: # [TCGA] # 12 69 197 232 # # TatI: # [WGTACW] # 241 269 # # TauI: # [GCSGC] # 117 180 213 # # Tsp45I: # [GTSAC] # 126 384 # # Tsp4CI*: # [ACNGT] # 57 151 302 # # TspDTI: # [ATGAA] # 18 44 62 79 307 # # TspEI: # [AATT] # 73 133 304 # # TspRI: # [CASTGNN] # 127 383 # # XhoII: # [RGATCY] # 142 368 # # XmnI: # [GAANNNNTTC] # 36 192 282 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 510 # Reported_sequences: 1 # Reported_hitcount: 103 #---------------------------------------