Restriction Fragments for YDL071C



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YDL071C on chromosome IV from coordinates 331040 to 330666.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24526.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 10 + Csp6I GTAC 7 9 . . 7 10 + RsaI GTAC 8 8 . . 11 15 - BsmAI GTCTC 5 9 . . 18 21 + CviJI RGCY 19 19 . . 19 23 + TauI GCSGC 22 19 . . 19 23 + BlsI GCNGC 21 20 . . 19 23 + BisI GCNGC 20 21 . . 20 23 + AciI CCGC 20 22 . . 22 27 + NmeAIII GCCGAG 48 46 . . 23 28 + SecI* CCNNGG 23 27 . . 26 29 + SetI ASST 29 25 . . 25 28 - MnlI CCTC 18 17 . . 62 65 + AciI CCGC 62 64 . . 63 66 + FnuDII* CGCG 64 64 . . 90 93 + CviJI RGCY 91 91 . . 89 94 - TsoI TARCCA 79 77 . . 95 98 + AciI CCGC 95 97 . . 96 99 + FnuDII* CGCG 97 97 . . 97 100 + Hin6I GCGC 97 99 . . 97 100 + GlaI GCGC 98 98 . . 97 100 + HhaI GCGC 99 97 . . 99 104 + Cac8I GCNNGC 101 101 . . 100 104 + FauI CCCGC 108 110 . . 101 104 + AciI CCGC 101 103 . . 119 124 + Ksp632I* CTCTTC 125 128 . . 123 127 + Hpy188I TCNGA 125 124 . . 120 124 - MboII GAAGA 112 111 . . 137 142 + CfrI YGGCCR 137 141 . . 138 141 + CviJI RGCY 139 139 . . 138 141 + HaeIII GGCC 139 139 . . 148 153 + HaeII RGCGCY 152 148 . . 149 152 + Hin6I GCGC 149 151 . . 149 152 + GlaI GCGC 150 150 . . 149 152 + HhaI GCGC 151 149 . . 155 158 + TaiI ACGT 158 154 . . 155 158 + SetI ASST 158 154 . . 155 158 + MaeII ACGT 155 157 . . 156 160 + Hpy99I CGWCG 160 155 . . 157 162 + SalI GTCGAC 157 161 . . 157 162 + Hpy166II GTNNAC 159 159 . . 157 162 + HindII GTYRAC 159 159 . . 157 162 + AccI GTMKAC 158 160 . . 158 161 + TaqI TCGA 158 160 . . 159 170 + BcgI CGANNNNNNTGC 148 146 182 180 172 176 + Tsp4CI* ACNGT 174 173 . . 177 181 + Tsp45I GTSAC 176 181 . . 177 181 + MaeIII GTNAC 176 181 . . 179 185 + TspRI CASTGNN 185 176 . . 180 184 + Tsp4CI* ACNGT 182 181 . . 183 188 + Hpy166II GTNNAC 185 185 . . 183 188 + SduI GDGCHC 187 183 . . 183 188 + BseSI GKGCMC 187 183 . . 183 188 + ApaLI GTGCAC 183 187 . . 183 188 + HgiAI* GWGCWC 187 183 . . 184 187 + CviRI* TGCA 185 185 . . 186 191 + BsiI* CACGAG 186 190 . . 201 205 + BsrI ACTGG 206 204 . . 205 215 + MwoI GCNNNNNNNGC 211 208 . . 214 217 + Hin6I GCGC 214 216 . . 214 217 + GlaI GCGC 215 215 . . 214 217 + HhaI GCGC 216 214 . . 217 220 + MnlI CCTC 227 226 . . 219 224 + Hpy178III* TCNNGA 220 222 . . 224 227 + TspEI AATT 223 227 . . 229 234 + SmlI CTYRAG 229 233 . . 229 234 + Bce83I* CTTGAG 250 248 . . 233 236 + CviJI RGCY 234 234 . . 235 238 + MnlI CCTC 245 244 . . 236 241 + Ksp632I* CTCTTC 242 245 . . 237 241 - MboII GAAGA 229 228 . . 252 255 + FnuDII* CGCG 253 253 . . 253 256 + Hin6I GCGC 253 255 . . 253 256 + GlaI GCGC 254 254 . . 253 256 + HhaI GCGC 255 253 . . 249 254 - BtgZI GCGATG 234 238 . . 265 269 + PleI GAGTC 273 274 . . 265 269 + MlyI GAGTC 274 274 . . 265 269 + HinfI GANTC 265 268 . . 265 275 + BplI GAGNNNNNCTC 256 251 288 283 265 275 - BplI GAGNNNNNCTC 288 283 256 251 277 281 + TauI GCSGC 280 277 . . 277 281 + BlsI GCNGC 279 278 . . 277 281 + BisI GCNGC 278 279 . . 278 281 + AciI CCGC 278 280 . . 289 292 + MseI TTAA 289 291 . . 326 331 + Hpy166II GTNNAC 328 328 . . 332 335 + MnlI CCTC 342 341 . . 328 332 - HphI GGTGA 320 319 . . 341 346 + Hpy166II GTNNAC 343 343 . . 351 354 + Hin6I GCGC 351 353 . . 351 354 + GlaI GCGC 352 352 . . 351 354 + HhaI GCGC 353 351 . . 352 355 + FnuDII* CGCG 353 353 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 158 217 # # AciI: # [CCGC] # 6 20 33 42 97 177 # # ApaLI: # [GTGCAC] # 183 192 # # Bce83I*: # [CTTGAG] # 125 250 # # BcgI: # [CGANNNNNNTGC] # 34 148 193 # # BisI: # [GCNGC] # 20 97 258 # # BlsI: # [GCNGC] # 21 96 258 # # BplI: # [GAGNNNNNCTC] # 0 0 32 87 256 # # BseSI: # [GKGCMC] # 187 188 # # BsiI*: # [CACGAG] # 186 189 # # BsmAI: # [GTCTC] # 5 370 # # BsrI: # [ACTGG] # 169 206 # # BtgZI: # [GCGATG] # 141 234 # # Cac8I: # [GCNNGC] # 101 274 # # CfrI: # [YGGCCR] # 137 238 # # Csp6I: # [GTAC] # 7 368 # # CviJI: # [RGCY] # 19 48 72 95 141 # # CviRI*: # [TGCA] # 185 190 # # FauI: # [CCCGC] # 108 267 # # FnuDII*: # [CGCG] # 22 33 64 100 156 # # GlaI: # [GCGC] # 23 39 52 65 98 98 # # HaeII: # [RGCGCY] # 152 223 # # HaeIII: # [GGCC] # 139 236 # # HgiAI*: # [GWGCWC] # 187 188 # # HhaI: # [GCGC] # 22 39 52 65 98 99 # # Hin6I: # [GCGC] # 24 39 52 65 97 98 # # HindII: # [GTYRAC] # 159 216 # # HinfI: # [GANTC] # 110 265 # # HphI: # [GGTGA] # 55 320 # # Hpy166II: # [GTNNAC] # 15 26 32 143 159 # # Hpy178III*: # [TCNNGA] # 155 220 # # Hpy188I: # [TCNGA] # 125 250 # # Hpy99I: # [CGWCG] # 160 215 # # Ksp632I*: # [CTCTTC] # 117 125 133 # # MaeII: # [ACGT] # 155 220 # # MaeIII: # [GTNAC] # 176 199 # # MboII: # [GAAGA] # 112 117 146 # # MlyI: # [GAGTC] # 101 274 # # MnlI: # [CCTC] # 18 18 33 97 209 # # MseI: # [TTAA] # 86 289 # # MwoI: # [GCNNNNNNNGC] # 164 211 # # NmeAIII: # [GCCGAG] # 48 327 # # PleI: # [GAGTC] # 102 273 # # RsaI: # [GTAC] # 8 367 # # SalI: # [GTCGAC] # 157 218 # # SduI: # [GDGCHC] # 187 188 # # SecI*: # [CCNNGG] # 23 352 # # SetI: # [ASST] # 29 129 217 # # SmlI: # [CTYRAG] # 146 229 # # TaiI: # [ACGT] # 158 217 # # TaqI: # [TCGA] # 158 217 # # TauI: # [GCSGC] # 22 95 258 # # TsoI: # [TARCCA] # 79 296 # # Tsp45I: # [GTSAC] # 176 199 # # Tsp4CI*: # [ACNGT] # 8 174 193 # # TspEI: # [AATT] # 152 223 # # TspRI: # [CASTGNN] # 185 190 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 375 # Reported_sequences: 1 # Reported_hitcount: 93 #---------------------------------------