Restriction Fragments for YDL050C



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YDL050C on chromosome IV from coordinates 364817 to 364446.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.15489.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 24 28 + MboII GAAGA 36 35 . . 36 40 + TfiI GAWTC 36 39 . . 36 40 + HinfI GANTC 36 39 . . 48 53 + SduI GDGCHC 52 48 . . 48 53 + HgiAI* GWGCWC 52 48 . . 53 56 + MnlI CCTC 63 62 . . 51 56 - BseRI GAGGAG 42 40 . . 67 72 + BsrDI GCAATG 74 72 . . 76 80 + MboII GAAGA 88 87 . . 79 83 + PleI GAGTC 87 88 . . 79 83 + MlyI GAGTC 88 88 . . 79 83 + HinfI GANTC 79 82 . . 76 81 - Ksp632I* CTCTTC 71 74 . . 83 86 + MnlI CCTC 93 92 . . 91 95 + HinfI GANTC 91 94 . . 93 98 + Ksp632I* CTCTTC 99 102 . . 91 95 - PleI GAGTC 85 86 . . 91 95 - MlyI GAGTC 85 85 . . 94 98 - MboII GAAGA 86 85 . . 102 106 - BceAI ACGGC 87 89 . . 110 114 - MboII GAAGA 102 101 . . 126 131 + ApoI RAATTY 126 130 . . 127 130 + TspEI AATT 126 130 . . 136 146 + FalI AAGNNNNNCTT 127 122 159 154 136 146 - FalI AAGNNNNNCTT 159 154 127 122 143 147 - MboII GAAGA 135 134 . . 151 156 + CfrI YGGCCR 151 155 . . 152 155 + CviJI RGCY 153 153 . . 152 155 + HaeIII GGCC 153 153 . . 157 160 + MseI TTAA 157 159 . . 153 157 - BceAI ACGGC 138 140 . . 162 167 + XhoII RGATCY 162 166 . . 162 166 + BinI* GGATC 170 171 . . 163 166 + DpnI GATC 164 164 . . 163 166 + BstKTI GATC 165 163 . . 163 172 + BsaBI GATNNNNATC 167 167 . . 163 166 + MboI GATC 162 166 . . 165 170 + Hpy178III* TCNNGA 166 168 . . 169 172 + DpnI GATC 170 170 . . 169 172 + BstKTI GATC 171 169 . . 169 172 + MboI GATC 168 172 . . 175 180 + NspBII* CMGCKG 177 177 . . 175 178 + AciI CCGC 175 177 . . 178 183 + Eco57MI CTGRAG 199 197 . . 178 183 + Eco57I CTGAAG 199 197 . . 194 197 + TaqI TCGA 194 196 . . 197 202 + StuI AGGCCT 199 199 . . 198 201 + CviJI RGCY 199 199 . . 198 201 + HaeIII GGCC 199 199 . . 196 199 - MnlI CCTC 189 188 . . 200 203 + MnlI CCTC 210 209 . . 218 222 + Tsp4CI* ACNGT 220 219 . . 238 242 + DdeI CTNAG 238 241 . . 238 242 + BspCNI CTCAG 251 249 . . 238 242 + BseMII CTCAG 252 250 . . 249 252 + SetI ASST 252 248 . . 250 254 + Hin4II* CCTTC 260 259 . . 254 259 + NdeI CATATG 255 257 . . 252 256 - TspDTI ATGAA 242 240 . . 263 268 + ApoI RAATTY 263 267 . . 264 267 + TspEI AATT 263 267 . . 274 278 + HinfI GANTC 274 277 . . 274 284 + Hin4I GAYNNNNNVTC 265 260 297 292 274 284 + Eam1105I GACNNNNNGTC 279 278 . . 270 275 - MmeI TCCRAC 251 249 . . 274 278 - PleI GAGTC 268 269 . . 274 278 - MlyI GAGTC 268 268 . . 274 284 - Hin4I GAYNNNNNVTC 297 292 265 260 305 310 + HindIII AAGCTT 305 309 . . 306 309 + SetI ASST 309 305 . . 306 309 + CviJI RGCY 307 307 . . 306 309 + AluI AGCT 307 307 . . 316 321 + Hpy178III* TCNNGA 317 319 . . 321 324 + TspEI AATT 320 324 . . 328 331 + MseI TTAA 328 330 . . 330 335 + ApoI RAATTY 330 334 . . 331 334 + TspEI AATT 330 334 . . 343 347 + Tsp4CI* ACNGT 345 344 . . 346 349 + Csp6I GTAC 346 348 . . 346 349 + RsaI GTAC 347 347 . . 352 355 + MseI TTAA 352 354 . . 354 359 + ApoI RAATTY 354 358 . . 355 358 + TspEI AATT 354 358 . . #--------------------------------------- # # # AciI: # [CCGC] # 175 197 # # AluI: # [AGCT] # 65 307 # # ApoI: # [RAATTY] # 18 24 67 126 137 # # BceAI: # [ACGGC] # 51 87 234 # # BinI*: # [GGATC] # 170 202 # # BsaBI: # [GATNNNNATC] # 167 205 # # BseMII: # [CTCAG] # 120 252 # # BseRI: # [GAGGAG] # 42 330 # # BspCNI: # [CTCAG] # 121 251 # # BsrDI: # [GCAATG] # 74 298 # # BstKTI: # [GATC] # 6 165 201 # # CfrI: # [YGGCCR] # 151 221 # # Csp6I: # [GTAC] # 26 346 # # CviJI: # [RGCY] # 46 65 108 153 # # DdeI: # [CTNAG] # 134 238 # # DpnI: # [GATC] # 6 164 202 # # Eam1105I: # [GACNNNNNGTC] # 93 279 # # Eco57I: # [CTGAAG] # 173 199 # # Eco57MI: # [CTGRAG] # 173 199 # # FalI: # [AAGNNNNNCTT] # 0 0 32 127 213 # # HaeIII: # [GGCC] # 46 153 173 # # HgiAI*: # [GWGCWC] # 52 320 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 75 265 # # Hin4II*: # [CCTTC] # 112 260 # # HindIII: # [AAGCTT] # 67 305 # # HinfI: # [GANTC] # 12 36 43 98 183 # # Hpy178III*: # [TCNNGA] # 55 151 166 # # Ksp632I*: # [CTCTTC] # 28 71 273 # # MboI: # [GATC] # 6 162 204 # # MboII: # [GAAGA] # 2 14 33 36 50 237 # # MlyI: # [GAGTC] # 3 85 104 180 # # MmeI: # [TCCRAC] # 121 251 # # MnlI: # [CCTC] # 21 30 63 96 162 # # MseI: # [TTAA] # 20 24 157 171 # # NdeI: # [CATATG] # 117 255 # # NspBII*: # [CMGCKG] # 177 195 # # PleI: # [GAGTC] # 2 85 104 181 # # RsaI: # [GTAC] # 25 347 # # SduI: # [GDGCHC] # 52 320 # # SetI: # [ASST] # 57 63 252 # # StuI: # [AGGCCT] # 173 199 # # TaqI: # [TCGA] # 178 194 # # TfiI: # [GAWTC] # 36 336 # # Tsp4CI*: # [ACNGT] # 27 125 220 # # TspDTI: # [ATGAA] # 130 242 # # TspEI: # [AATT] # 10 18 24 57 126 137 # # XhoII: # [RGATCY] # 162 210 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 372 # Reported_sequences: 1 # Reported_hitcount: 83 #---------------------------------------