Restriction Fragments for ARP2/YDL029W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARP2/YDL029W on chromosome IV from coordinates 399340 to 400638.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27952.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + NlaIV GGNNCC 5 5 . . 3 7 + BmgT120I GGNCC 4 5 . . 3 7 + AvaII GGWCC 3 6 . . 3 7 + AsuI* GGNCC 3 6 . . 17 22 + MfeI CAATTG 17 21 . . 18 21 + TspEI AATT 17 21 . . 52 55 - MnlI CCTC 45 44 . . 56 61 + MfeI CAATTG 56 60 . . 57 60 + TspEI AATT 56 60 . . 61 66 + SacI GAGCTC 65 61 . . 61 66 + Ecl136II GAGCTC 63 63 . . 61 66 + SduI GDGCHC 65 61 . . 61 66 + HgiJII* GRGCYC 65 61 . . 61 66 + HgiAI* GWGCWC 65 61 . . 62 65 + SetI ASST 65 61 . . 62 65 + CviJI RGCY 63 63 . . 62 65 + AluI AGCT 63 63 . . 67 72 + NlaIV GGNNCC 69 69 . . 67 72 + HgiCI* GGYRCC 67 71 . . 68 73 + SduI GDGCHC 72 68 . . 68 73 + BseSI GKGCMC 72 68 . . 79 82 + CviJI RGCY 80 80 . . 85 95 + MwoI GCNNNNNNNGC 91 88 . . 86 89 + MnlI CCTC 96 95 . . 93 96 + CviRI* TGCA 94 94 . . 121 126 + VspI ATTAAT 122 124 . . 122 125 + MseI TTAA 122 124 . . 129 134 + TsoI TARCCA 145 143 . . 132 136 + BccI CCATC 140 141 . . 135 140 + Hpy178III* TCNNGA 136 138 . . 137 140 + TaqI TCGA 137 139 . . 147 157 + EcoNI CCTNNNNNAGG 151 152 . . 147 157 + BsiYI* CCNNNNNNNGG 153 150 . . 150 155 + BclI TGATCA 150 154 . . 151 154 + DpnI GATC 152 152 . . 151 154 + BstKTI GATC 153 151 . . 151 154 + MboI GATC 150 154 . . 158 161 + Csp6I GTAC 158 160 . . 158 161 + RsaI GTAC 159 159 . . 160 164 + BsrI ACTGG 165 163 . . 174 177 + TspEI AATT 173 177 . . 184 189 + Cac8I GCNNGC 186 186 . . 192 197 + ApoI RAATTY 192 196 . . 193 196 + TspEI AATT 192 196 . . 206 211 + AflIII ACRYGT 206 210 . . 208 211 + TaiI ACGT 211 207 . . 208 211 + SetI ASST 211 207 . . 208 211 + MaeII ACGT 208 210 . . 214 218 + Hin4II* CCTTC 224 223 . . 227 232 + Hpy166II GTNNAC 229 229 . . 227 232 + AccI GTMKAC 228 230 . . 233 237 + BccI CCATC 241 242 . . 233 243 + BsiYI* CCNNNNNNNGG 239 236 . . 236 241 + Hpy178III* TCNNGA 237 239 . . 237 242 + SmlI CTYRAG 237 241 . . 237 242 + Bce83I* CTTGAG 258 256 . . 240 243 - MnlI CCTC 233 232 . . 243 248 + EciI GGCGGA 259 257 . . 247 251 + MboII GAAGA 259 258 . . 244 247 - AciI CCGC 244 246 . . 252 255 + TaiI ACGT 255 251 . . 252 255 + SetI ASST 255 251 . . 252 255 + MaeII ACGT 252 254 . . 256 266 + MwoI GCNNNNNNNGC 262 259 . . 256 261 + Cac8I GCNNGC 258 258 . . 274 277 + MseI TTAA 274 276 . . 292 296 + HphI GGTGA 304 303 . . 298 301 - MnlI CCTC 291 290 . . 323 326 + CviRI* TGCA 324 324 . . 347 351 + Tsp4CI* ACNGT 349 348 . . 356 359 + MseI TTAA 356 358 . . 361 364 + TspEI AATT 360 364 . . 401 404 + TaqI TCGA 401 403 . . 409 413 + TspDTI ATGAA 424 422 . . 418 422 + BccI CCATC 426 427 . . 419 423 - FokI GGATG 405 409 . . 419 423 - BseGI GGATG 418 416 . . 424 427 + SetI ASST 427 423 . . 425 428 + MnlI CCTC 435 434 . . 427 432 + MmeI TCCRAC 452 450 . . 431 435 + Tsp4CI* ACNGT 433 432 . . 448 452 + TspGWI ACGGA 463 461 . . 448 458 + BsaXI ACNNNNNCTCC 438 435 468 465 450 455 + NlaIV GGNNCC 452 452 . . 453 456 + SetI ASST 456 452 . . 454 457 + MnlI CCTC 464 463 . . 460 464 + TspDTI ATGAA 475 473 . . 462 466 + TfiI GAWTC 462 465 . . 462 466 + HinfI GANTC 462 465 . . 466 471 + NspBII* CMGCKG 468 468 . . 466 469 + AciI CCGC 466 468 . . 480 491 - TstI CACNNNNNNTCC 504 499 472 467 494 497 + SetI ASST 497 493 . . 493 496 - MnlI CCTC 486 485 . . 502 507 + AsuII TTCGAA 503 505 . . 503 506 + TaqI TCGA 503 505 . . 493 504 - PpiI GAACNNNNNCTC 516 511 484 479 520 525 + EciI GGCGGA 536 534 . . 521 524 - AciI CCGC 521 523 . . 523 533 - BsaXI ACNNNNNCTCC 545 542 515 512 535 545 + MwoI GCNNNNNNNGC 541 538 . . 536 540 + BccI CCATC 544 545 . . 537 541 - FokI GGATG 523 527 . . 537 541 - BseGI GGATG 536 534 . . 543 546 + SetI ASST 546 542 . . 543 546 + CviJI RGCY 544 544 . . 543 546 + AluI AGCT 544 544 . . 544 554 + MwoI GCNNNNNNNGC 550 547 . . 550 553 + MaeI CTAG 550 552 . . 553 563 + MwoI GCNNNNNNNGC 559 556 . . 558 561 + Csp6I GTAC 558 560 . . 558 561 + RsaI GTAC 559 559 . . 567 570 + SetI ASST 570 566 . . 577 582 + Hpy178III* TCNNGA 578 580 . . 574 578 - MboII GAAGA 566 565 . . 573 578 - BbvII* GAAGAC 566 570 . . 575 580 - Eco57MI CTGRAG 560 558 . . 575 580 - Eco57I CTGAAG 560 558 . . 581 585 + PleI GAGTC 589 590 . . 581 585 + MlyI GAGTC 590 590 . . 581 585 + HinfI GANTC 581 584 . . 585 589 + Hpy99I CGWCG 589 584 . . 588 592 + Hpy99I CGWCG 592 587 . . 590 593 + TaqI TCGA 590 592 . . 580 590 - BsaXI ACNNNNNCTCC 602 599 572 569 581 591 - Hin4I GAYNNNNNVTC 604 599 572 567 592 596 + TfiI GAWTC 592 595 . . 592 596 + HinfI GANTC 592 595 . . 595 600 + BetI* WCCGGW 595 599 . . 596 599 + HpaII CCGG 596 598 . . 598 602 + HphI GGTGA 610 609 . . 599 603 + Tsp45I GTSAC 598 603 . . 599 603 + MaeIII GTNAC 598 603 . . 602 606 + Tsp4CI* ACNGT 604 603 . . 607 611 + MaeIII GTNAC 606 611 . . 619 623 - BslFI GGGAC 604 608 . . 625 630 + Hpy166II GTNNAC 627 627 . . 622 626 - BsrI ACTGG 622 620 . . 621 626 - BfiI ACTGGG 616 615 . . 631 635 + TfiI GAWTC 631 634 . . 631 635 + HinfI GANTC 631 634 . . 646 649 + CviJI RGCY 647 647 . . 652 655 + MseI TTAA 652 654 . . 659 663 + MboII GAAGA 671 670 . . 673 676 - AciI CCGC 673 675 . . 673 677 - FauI CCCGC 666 668 . . 683 686 + TaiI ACGT 686 682 . . 683 686 + SetI ASST 686 682 . . 683 686 + MaeII ACGT 683 685 . . 680 684 - BsmAI GTCTC 674 678 . . 685 689 + MaeIII GTNAC 684 689 . . 680 685 - Esp3I CGTCTC 674 678 . . 689 692 + MaeI CTAG 689 691 . . 703 706 + DpnI GATC 704 704 . . 703 706 + BstKTI GATC 705 703 . . 703 706 + MboI GATC 702 706 . . 715 719 + Hpy99I CGWCG 719 714 . . 720 725 - TsoI TARCCA 710 708 . . 725 730 + EcoT22I ATGCAT 729 725 . . 726 729 + CviRI* TGCA 727 727 . . 731 734 + MseI TTAA 731 733 . . 740 745 + PstI CTGCAG 744 740 . . 740 745 + SfeI* CTRYAG 740 744 . . 741 744 + CviRI* TGCA 742 742 . . 748 753 + AsuII TTCGAA 749 751 . . 749 752 + TaqI TCGA 749 751 . . 754 758 + Tsp4CI* ACNGT 756 755 . . 762 766 + Hpy188I TCNGA 764 763 . . 759 763 - HgaI GACGC 748 753 . . 776 779 + TspEI AATT 775 779 . . 791 796 + NdeI CATATG 792 794 . . 789 793 - TspDTI ATGAA 779 777 . . 803 806 + SetI ASST 806 802 . . 805 808 + MaeI CTAG 805 807 . . 815 818 + TspEI AATT 814 818 . . 819 822 + CviJI RGCY 820 820 . . 821 824 + MaeI CTAG 821 823 . . 818 823 - TsoI TARCCA 808 806 . . 833 836 + AciI CCGC 833 835 . . 844 848 + TfiI GAWTC 844 847 . . 844 848 + HinfI GANTC 844 847 . . 855 859 + MaeIII GTNAC 854 859 . . 862 866 - BccI CCATC 856 857 . . 872 883 + CspCI CAANNNNNGTGG 860 858 895 893 882 886 + BslFI GGGAC 896 900 . . 902 911 + MslI CAYNNNNRTG 906 906 . . 917 922 + MmeI TCCRAC 942 940 . . 921 924 + SetI ASST 924 920 . . 921 927 + SexAI ACCWGGT 921 926 . . 922 926 + BssKI CCNGG 921 926 . . 922 926 + BseBI CCWGG 923 924 . . 922 926 + ScrFI CCNGG 923 924 . . 922 926 + EcoRII CCWGG 921 926 . . 931 936 + Hpy166II GTNNAC 933 933 . . 931 936 + HindII GTYRAC 933 933 . . 935 938 + TaiI ACGT 938 934 . . 935 938 + SetI ASST 938 934 . . 935 938 + MaeII ACGT 935 937 . . 945 948 + SetI ASST 948 944 . . 946 950 + BssKI CCNGG 945 950 . . 946 950 + BseBI CCWGG 947 948 . . 946 950 + ScrFI CCNGG 947 948 . . 946 950 + EcoRII CCWGG 945 950 . . 946 956 + BsiYI* CCNNNNNNNGG 952 949 . . 956 961 + Cac8I GCNNGC 958 958 . . 959 962 + SetI ASST 962 958 . . 959 962 + CviJI RGCY 960 960 . . 959 962 + AluI AGCT 960 960 . . 968 971 + MseI TTAA 968 970 . . 973 977 + Tsp4CI* ACNGT 975 974 . . 977 980 + CviRI* TGCA 978 978 . . 984 987 + CviJI RGCY 985 985 . . 994 999 + EcoRV GATATC 996 996 . . 998 1002 + Hpy188I TCNGA 1000 999 . . 1008 1018 + EcoNI CCTNNNNNAGG 1012 1013 . . 1008 1018 + BsiYI* CCNNNNNNNGG 1014 1011 . . 1017 1020 + CviJI RGCY 1018 1018 . . 1017 1020 + HaeIII GGCC 1018 1018 . . 1032 1035 + SetI ASST 1035 1031 . . 1047 1050 + Csp6I GTAC 1047 1049 . . 1047 1050 + RsaI GTAC 1048 1048 . . 1050 1056 + PasI CCCWGGG 1051 1054 . . 1050 1055 + SecI* CCNNGG 1050 1054 . . 1051 1055 + BssKI CCNGG 1050 1055 . . 1051 1055 + BseBI CCWGG 1052 1053 . . 1051 1056 + SecI* CCNNGG 1051 1055 . . 1051 1055 + ScrFI CCNGG 1052 1053 . . 1051 1055 + EcoRII CCWGG 1050 1055 . . 1055 1058 + CviJI RGCY 1056 1056 . . 1056 1060 + BlsI GCNGC 1058 1057 . . 1056 1060 + BisI GCNGC 1057 1058 . . 1056 1060 + TseI GCWGC 1056 1059 . . 1060 1064 + Hin4II* CCTTC 1070 1069 . . 1056 1060 - BbvI GCAGC 1043 1047 . . 1063 1066 + TaqI TCGA 1063 1065 . . 1063 1068 + Hpy178III* TCNNGA 1064 1066 . . 1079 1082 + TspEI AATT 1078 1082 . . 1088 1091 + TspEI AATT 1087 1091 . . 1120 1124 + Hin4II* CCTTC 1130 1129 . . 1123 1128 + Hpy178III* TCNNGA 1124 1126 . . 1135 1140 + ApoI RAATTY 1135 1139 . . 1136 1139 + TspEI AATT 1135 1139 . . 1149 1152 + TspEI AATT 1148 1152 . . 1153 1157 + MboII GAAGA 1165 1164 . . 1155 1160 + XhoII RGATCY 1155 1159 . . 1156 1159 + DpnI GATC 1157 1157 . . 1156 1159 + BstKTI GATC 1158 1156 . . 1156 1159 + MboI GATC 1155 1159 . . 1159 1162 + MnlI CCTC 1169 1168 . . 1156 1160 - BinI* GGATC 1150 1151 . . 1162 1167 + StyI CCWWGG 1162 1166 . . 1162 1167 + SecI* CCNNGG 1162 1166 . . 1174 1179 + NdeI CATATG 1175 1177 . . 1183 1187 - TspDTI ATGAA 1173 1171 . . 1192 1197 + NlaIV GGNNCC 1194 1194 . . 1192 1197 + HgiCI* GGYRCC 1192 1196 . . 1195 1205 + MwoI GCNNNNNNNGC 1201 1198 . . 1195 1199 - BceAI ACGGC 1180 1182 . . 1203 1206 + SetI ASST 1206 1202 . . 1203 1206 + CviJI RGCY 1204 1204 . . 1203 1206 + AluI AGCT 1204 1204 . . 1205 1208 + MaeI CTAG 1205 1207 . . 1212 1215 + FatI CATG 1211 1215 . . 1212 1215 + NlaIII CATG 1215 1211 . . 1212 1215 + CviAII CATG 1212 1214 . . 1215 1218 + CviJI RGCY 1216 1216 . . 1229 1234 + BspLU11I* ACATGT 1229 1233 . . 1229 1234 + NspI RCATGY 1233 1229 . . 1229 1234 + AflIII ACRYGT 1229 1233 . . 1230 1233 + FatI CATG 1229 1233 . . 1230 1233 + NlaIII CATG 1233 1229 . . 1230 1233 + CviAII CATG 1230 1232 . . 1241 1245 + DdeI CTNAG 1241 1244 . . 1245 1255 + MwoI GCNNNNNNNGC 1251 1248 . . 1262 1267 + Cac8I GCNNGC 1264 1264 . . 1264 1268 + BmgT120I GGNCC 1265 1266 . . 1264 1268 + AsuI* GGNCC 1264 1267 . . 1265 1268 + CviJI RGCY 1266 1266 . . 1265 1268 + HaeIII GGCC 1266 1266 . . 1262 1265 - AciI CCGC 1262 1264 . . 1262 1266 - FauI CCCGC 1255 1257 . . 1267 1271 + BccI CCATC 1275 1276 . . 1272 1275 + CviRI* TGCA 1273 1273 . . 1273 1278 + BsrDI GCAATG 1280 1278 . . 1282 1287 + ApoI RAATTY 1282 1286 . . 1283 1286 + TspEI AATT 1282 1286 . . 1288 1292 + BmgT120I GGNCC 1289 1290 . . 1288 1292 + AvaII GGWCC 1288 1291 . . 1288 1292 + AsuI* GGNCC 1288 1291 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 228 1071 # # AciI: # [CCGC] # 37 55 152 160 222 244 # 429 # # AflIII: # [ACRYGT] # 70 206 1023 # # AluI: # [AGCT] # 63 95 244 416 481 # # ApoI: # [RAATTY] # 17 147 192 943 # # AsuI*: # [GGNCC] # 3 11 24 1261 # # AsuII: # [TTCGAA] # 246 503 550 # # AvaII: # [GGWCC] # 3 11 1285 # # BbvI: # [GCAGC] # 256 1043 # # BbvII*: # [GAAGAC] # 566 733 # # BccI: # [CCATC] # 24 101 118 140 185 312 # 419 # # Bce83I*: # [CTTGAG] # 258 1041 # # BceAI: # [ACGGC] # 119 1180 # # BclI: # [TGATCA] # 150 1149 # # BetI*: # [WCCGGW] # 595 704 # # BfiI: # [ACTGGG] # 616 683 # # BinI*: # [GGATC] # 149 1150 # # BisI: # [GCNGC] # 242 1057 # # BlsI: # [GCNGC] # 241 1058 # # BmgT120I: # [GGNCC] # 4 10 24 1261 # # BsaXI: # [ACNNNNNCTCC] # 27 30 30 30 47 438 # 697 # # BseBI: # [CCWGG] # 24 105 247 923 # # BseGI: # [GGATG] # 118 418 763 # # BseSI: # [GKGCMC] # 72 1227 # # BsiYI*: # [CCNNNNNNNGG] # 62 86 153 285 713 # # BslFI: # [GGGAC] # 292 403 604 # # BsmAI: # [GTCTC] # 625 674 # # BspLU11I*: # [ACATGT] # 70 1229 # # BsrDI: # [GCAATG] # 19 1280 # # BsrI: # [ACTGG] # 165 457 677 # # BssKI: # [CCNGG] # 24 105 249 921 # # BstKTI: # [GATC] # 141 153 453 552 # # Cac8I: # [GCNNGC] # 35 72 186 306 700 # # Csp6I: # [GTAC] # 158 252 400 489 # # CspCI: # [CAANNNNNGTGG] # 35 404 860 # # CviAII: # [CATG] # 18 69 1212 # # CviJI: # [RGCY] # 12 17 25 33 33 38 # 50 63 103 140 148 173 # 464 # # CviRI*: # [TGCA] # 15 26 94 230 236 295 # 403 # # DdeI: # [CTNAG] # 58 1241 # # DpnI: # [GATC] # 142 152 453 552 # # EciI: # [GGCGGA] # 259 277 763 # # Ecl136II: # [GAGCTC] # 63 1236 # # Eco57I: # [CTGAAG] # 560 739 # # Eco57MI: # [CTGRAG] # 560 739 # # EcoNI: # [CCTNNNNNAGG] # 151 287 861 # # EcoRII: # [CCWGG] # 24 105 249 921 # # EcoRV: # [GATATC] # 303 996 # # EcoT22I: # [ATGCAT] # 570 729 # # Esp3I: # [CGTCTC] # 625 674 # # FatI: # [CATG] # 18 70 1211 # # FauI: # [CCCGC] # 44 589 666 # # FokI: # [GGATG] # 118 405 776 # # HaeIII: # [GGCC] # 33 248 1018 # # HgaI: # [GACGC] # 551 748 # # HgiAI*: # [GWGCWC] # 65 1234 # # HgiCI*: # [GGYRCC] # 67 107 1125 # # HgiJII*: # [GRGCYC] # 65 1234 # # Hin4I: # [GAYNNNNNVTC] # 32 572 695 # # Hin4II*: # [CCTTC] # 60 169 224 846 # # HindII: # [GTYRAC] # 366 933 # # HinfI: # [GANTC] # 11 39 119 213 455 462 # # HpaII: # [CCGG] # 596 703 # # HphI: # [GGTGA] # 304 306 689 # # Hpy166II: # [GTNNAC] # 229 306 366 398 # # Hpy178III*: # [TCNNGA] # 60 101 136 175 341 486 # # Hpy188I: # [TCNGA] # 236 299 764 # # Hpy99I: # [CGWCG] # 3 127 580 589 # # MaeI: # [CTAG] # 16 94 116 139 384 550 # # MaeII: # [ACGT] # 44 208 252 364 431 # # MaeIII: # [GTNAC] # 8 78 170 445 598 # # MboI: # [GATC] # 144 150 453 552 # # MboII: # [GAAGA] # 105 134 259 307 494 # # MfeI: # [CAATTG] # 17 39 1243 # # MlyI: # [GAGTC] # 590 709 # # MmeI: # [TCCRAC] # 357 452 490 # # MnlI: # [CCTC] # 22 29 45 51 58 130 # 137 144 683 # # MseI: # [TTAA] # 79 82 122 152 237 296 # 331 # # MslI: # [CAYNNNNRTG] # 393 906 # # MwoI: # [GCNNNNNNNGC] # 9 9 48 50 91 171 # 279 642 # # NdeI: # [CATATG] # 124 383 792 # # NlaIII: # [CATG] # 18 66 1215 # # NlaIV: # [GGNNCC] # 5 64 105 383 742 # # NspBII*: # [CMGCKG] # 468 831 # # NspI: # [RCATGY] # 66 1233 # # PasI: # [CCCWGGG] # 248 1051 # # PleI: # [GAGTC] # 589 710 # # PpiI: # [GAACNNNNNCTC] # 32 484 783 # # PstI: # [CTGCAG] # 555 744 # # RsaI: # [GTAC] # 159 251 400 489 # # SacI: # [GAGCTC] # 65 1234 # # ScrFI: # [CCNGG] # 24 105 247 923 # # SduI: # [GDGCHC] # 7 65 1227 # # SecI*: # [CCNNGG] # 1 111 137 1050 # # SetI: # [ASST] # 10 14 14 24 29 41 # 44 49 65 73 93 116 # 118 120 146 171 172 # # SexAI: # [ACCWGGT] # 378 921 # # SfeI*: # [CTRYAG] # 559 740 # # SmlI: # [CTYRAG] # 237 1062 # # StyI: # [CCWWGG] # 137 1162 # # TaiI: # [ACGT] # 44 211 252 361 431 # # TaqI: # [TCGA] # 87 102 137 159 236 264 # 314 # # TfiI: # [GAWTC] # 39 130 213 455 462 # # TseI: # [GCWGC] # 243 1056 # # TsoI: # [TARCCA] # 98 145 491 565 # # Tsp45I: # [GTSAC] # 598 701 # # Tsp4CI*: # [ACNGT] # 84 152 171 219 324 349 # # TspDTI: # [ATGAA] # 51 126 304 394 424 # # TspEI: # [AATT] # 9 13 17 17 19 39 # 39 48 117 134 168 264 # 415 # # TspGWI: # [ACGGA] # 463 836 # # TstI: # [CACNNNNNNTCC] # 32 472 795 # # VspI: # [ATTAAT] # 122 1177 # # XhoII: # [RGATCY] # 144 1155 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1299 # Reported_sequences: 1 # Reported_hitcount: 289 #---------------------------------------