Restriction Fragments for TSC13/YDL015C



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The currently selected gene/sequence is :

TSC13/YDL015C on chromosome IV from coordinates 426934 to 426002.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.13014.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 19 22 + CviJI RGCY 20 20 . . 20 24 + TauI GCSGC 23 20 . . 20 24 + BlsI GCNGC 22 21 . . 20 24 + BisI GCNGC 21 22 . . 21 24 + AciI CCGC 21 23 . . 21 26 + BsrBI CCGCTC 23 23 . . 34 37 + MseI TTAA 34 36 . . 38 42 + BslFI GGGAC 52 56 . . 42 48 + TspRI CASTGNN 48 39 . . 48 51 + TspEI AATT 47 51 . . 65 68 + CviJI RGCY 66 66 . . 105 110 + TsoI TARCCA 121 119 . . 122 127 + TatI WGTACW 122 126 . . 123 126 + Csp6I GTAC 123 125 . . 123 126 + RsaI GTAC 124 124 . . 136 139 + MseI TTAA 136 138 . . 139 142 + SetI ASST 142 138 . . 151 155 + TfiI GAWTC 151 154 . . 151 155 + HinfI GANTC 151 154 . . 165 170 + BetI* WCCGGW 165 169 . . 166 169 + HpaII CCGG 166 168 . . 175 179 + Hpy188I TCNGA 177 176 . . 178 182 + TfiI GAWTC 178 181 . . 178 182 + HinfI GANTC 178 181 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 193 197 + MboII GAAGA 205 204 . . 198 201 + CviJI RGCY 199 199 . . 193 198 - Ksp632I* CTCTTC 188 191 . . 196 199 - MnlI CCTC 189 188 . . 205 209 + HinfI GANTC 205 208 . . 205 209 - PleI GAGTC 199 200 . . 205 209 - MlyI GAGTC 199 199 . . 214 219 + EcoRI GAATTC 214 218 . . 214 219 + ApoI RAATTY 214 218 . . 215 218 + TspEI AATT 214 218 . . 218 222 - MboII GAAGA 210 209 . . 220 224 - TspDTI ATGAA 210 208 . . 234 240 + SanDI GGGWCCC 235 238 . . 234 240 + PpuMI RGGWCCY 235 238 . . 234 240 + DraII RGGNCCY 235 238 . . 234 239 + NlaIV GGNNCC 236 236 . . 235 240 + NlaIV GGNNCC 237 237 . . 235 239 + BmgT120I GGNCC 236 237 . . 235 239 + AvaII GGWCC 235 238 . . 235 239 + AsuI* GGNCC 235 238 . . 236 240 - BslFI GGGAC 221 225 . . 242 247 + ApoI RAATTY 242 246 . . 243 246 + TspEI AATT 242 246 . . 248 251 + FatI CATG 247 251 . . 248 251 + NlaIII CATG 251 247 . . 248 251 + CviAII CATG 248 250 . . 246 250 - TspDTI ATGAA 236 234 . . 260 264 - MboII GAAGA 252 251 . . 259 264 - BbvII* GAAGAC 252 256 . . 279 284 + NlaIV GGNNCC 281 281 . . 280 284 + BmgT120I GGNCC 281 282 . . 280 284 + AvaII GGWCC 280 283 . . 280 284 + AsuI* GGNCC 280 283 . . 283 287 - BsrI ACTGG 283 281 . . 292 297 + Hpy166II GTNNAC 294 294 . . 328 332 + Tsp4CI* ACNGT 330 329 . . 326 337 - CspCI CAANNNNNGTGG 350 348 315 313 341 345 - BccI CCATC 335 336 . . 347 351 + Tsp4CI* ACNGT 349 348 . . 348 354 + TspRI CASTGNN 354 345 . . 352 357 + Cac8I GCNNGC 354 354 . . 352 357 + BmtI GCTAGC 356 352 . . 352 357 + NheI GCTAGC 352 356 . . 353 356 + MaeI CTAG 353 355 . . 355 358 + SetI ASST 358 354 . . 355 358 + CviJI RGCY 356 356 . . 355 358 + AluI AGCT 356 356 . . 358 363 + MmeI TCCRAC 383 381 . . 358 362 + Hpy188I TCNGA 360 359 . . 375 380 + AhaIII* TTTAAA 377 377 . . 376 379 + MseI TTAA 376 378 . . 387 390 + CviRI* TGCA 388 388 . . 397 400 + MseI TTAA 397 399 . . 399 402 + TspEI AATT 398 402 . . 433 436 + SetI ASST 436 432 . . 442 447 + Hpy166II GTNNAC 444 444 . . 444 448 - HphI GGTGA 436 435 . . 449 452 + TspEI AATT 448 452 . . 459 462 + SetI ASST 462 458 . . 459 462 + CviJI RGCY 460 460 . . 459 462 + AluI AGCT 460 460 . . 471 474 + TspEI AATT 470 474 . . 479 482 + SetI ASST 482 478 . . 490 493 + TspEI AATT 489 493 . . 498 508 + BsiYI* CCNNNNNNNGG 504 501 . . 503 507 + BsrI ACTGG 508 506 . . 503 508 + BfiI ACTGGG 513 512 . . 511 515 + DdeI CTNAG 511 514 . . 517 522 + Eco31I GGTCTC 523 527 . . 518 522 + BsmAI GTCTC 523 527 . . 515 518 - AciI CCGC 515 517 . . 525 529 - TspDTI ATGAA 515 513 . . 533 537 + MaeIII GTNAC 532 537 . . 541 544 + CviJI RGCY 542 542 . . 540 545 - TsoI TARCCA 530 528 . . 545 549 + BceAI ACGGC 561 563 . . 547 550 + CviJI RGCY 548 548 . . 561 566 + BsmI GAATGC 567 565 . . 566 570 + DdeI CTNAG 566 569 . . 585 589 - TspDTI ATGAA 575 573 . . 596 599 + TspEI AATT 595 599 . . 600 604 + FokI GGATG 613 617 . . 600 604 + BseGI GGATG 606 604 . . 606 611 + SmlI CTYRAG 606 610 . . 606 611 + Bce83I* CTTGAG 627 625 . . 610 615 + TatI WGTACW 610 614 . . 611 614 + Csp6I GTAC 611 613 . . 611 614 + RsaI GTAC 612 612 . . 615 620 + VspI ATTAAT 616 618 . . 616 619 + MseI TTAA 616 618 . . 618 621 + TspEI AATT 617 621 . . 637 641 + Hpy188I TCNGA 639 638 . . 665 668 + MseI TTAA 665 667 . . 668 671 + TspEI AATT 667 671 . . 672 675 + Hin6I GCGC 672 674 . . 672 675 + GlaI GCGC 673 673 . . 672 675 + HhaI GCGC 674 672 . . 682 686 + HphI GGTGA 694 693 . . 683 687 + Tsp45I GTSAC 682 687 . . 683 687 + MaeIII GTNAC 682 687 . . 700 703 + FatI CATG 699 703 . . 700 703 + NlaIII CATG 703 699 . . 700 703 + CviAII CATG 700 702 . . 704 708 + MaeIII GTNAC 703 708 . . 710 714 + DdeI CTNAG 710 713 . . 713 718 + XhoII RGATCY 713 717 . . 714 717 + DpnI GATC 715 715 . . 714 717 + BstKTI GATC 716 714 . . 714 717 + MboI GATC 713 717 . . 714 718 - BinI* GGATC 708 709 . . 716 720 - TspGWI ACGGA 706 704 . . 721 725 - BslFI GGGAC 706 710 . . 729 733 + TfiI GAWTC 729 732 . . 729 733 + HinfI GANTC 729 732 . . 735 738 + SetI ASST 738 734 . . 795 800 + Hpy166II GTNNAC 797 797 . . 817 820 + TspEI AATT 816 820 . . 826 830 - BceAI ACGGC 811 813 . . 841 845 + Tsp4CI* ACNGT 843 842 . . 852 855 + SetI ASST 855 851 . . 852 855 + CviJI RGCY 853 853 . . 852 855 + AluI AGCT 853 853 . . 861 864 + Csp6I GTAC 861 863 . . 861 864 + RsaI GTAC 862 862 . . 866 869 + FatI CATG 865 869 . . 866 869 + NlaIII CATG 869 865 . . 866 869 + CviAII CATG 866 868 . . 869 874 + SduI GDGCHC 873 869 . . 869 874 + HgiJII* GRGCYC 873 869 . . 870 873 + CviJI RGCY 871 871 . . 902 906 + MboII GAAGA 914 913 . . 902 907 - Ksp632I* CTCTTC 897 900 . . 902 908 - SapI GCTCTTC 897 900 . . 911 916 + Hpy178III* TCNNGA 912 914 . . 907 912 - BsmI GAATGC 907 905 . . 915 919 + TfiI GAWTC 915 918 . . 915 919 + HinfI GANTC 915 918 . . #--------------------------------------- # # # AciI: # [CCGC] # 21 418 494 # # AhaIII*: # [TTTAAA] # 377 556 # # AluI: # [AGCT] # 80 104 356 393 # # ApoI: # [RAATTY] # 28 214 691 # # AsuI*: # [GGNCC] # 45 235 653 # # AvaII: # [GGWCC] # 45 235 653 # # BbvII*: # [GAAGAC] # 252 681 # # BccI: # [CCATC] # 335 598 # # Bce83I*: # [CTTGAG] # 306 627 # # BceAI: # [ACGGC] # 122 250 561 # # BetI*: # [WCCGGW] # 165 768 # # BfiI: # [ACTGGG] # 420 513 # # BinI*: # [GGATC] # 225 708 # # BisI: # [GCNGC] # 21 912 # # BlsI: # [GCNGC] # 22 911 # # BmgT120I: # [GGNCC] # 45 236 652 # # BmtI: # [GCTAGC] # 356 577 # # BseGI: # [GGATG] # 327 606 # # BsiYI*: # [CCNNNNNNNGG] # 429 504 # # BslFI: # [GGGAC] # 52 169 227 485 # # BsmAI: # [GTCTC] # 410 523 # # BsmI: # [GAATGC] # 26 340 567 # # BsrBI: # [CCGCTC] # 23 910 # # BsrI: # [ACTGG] # 225 283 425 # # BstKTI: # [GATC] # 217 716 # # Cac8I: # [GCNNGC] # 354 579 # # Csp6I: # [GTAC] # 72 123 250 488 # # CspCI: # [CAANNNNNGTGG] # 35 315 583 # # CviAII: # [CATG] # 67 166 248 452 # # CviJI: # [RGCY] # 6 18 20 46 62 82 # 104 133 157 305 # # CviRI*: # [TGCA] # 388 545 # # DdeI: # [CTNAG] # 55 144 223 511 # # DpnI: # [GATC] # 218 715 # # DraII: # [RGGNCCY] # 235 698 # # Eco31I: # [GGTCTC] # 410 523 # # EcoRI: # [GAATTC] # 214 719 # # FatI: # [CATG] # 68 166 247 452 # # FokI: # [GGATG] # 320 613 # # GlaI: # [GCGC] # 260 673 # # HgiJII*: # [GRGCYC] # 60 873 # # HhaI: # [GCGC] # 259 674 # # Hin6I: # [GCGC] # 261 672 # # HinfI: # [GANTC] # 18 27 27 151 186 524 # # HpaII: # [CCGG] # 166 767 # # HphI: # [GGTGA] # 239 258 436 # # Hpy166II: # [GTNNAC] # 136 150 294 353 # # Hpy178III*: # [TCNNGA] # 21 190 722 # # Hpy188I: # [TCNGA] # 177 183 279 294 # # Ksp632I*: # [CTCTTC] # 36 188 709 # # MaeI: # [CTAG] # 353 580 # # MaeIII: # [GTNAC] # 21 150 230 532 # # MboI: # [GATC] # 220 713 # # MboII: # [GAAGA] # 5 19 42 205 662 # # MlyI: # [GAGTC] # 199 734 # # MmeI: # [TCCRAC] # 383 550 # # MnlI: # [CCTC] # 189 744 # # MseI: # [TTAA] # 21 34 49 102 219 240 # 268 # # NheI: # [GCTAGC] # 352 581 # # NlaIII: # [CATG] # 64 166 251 452 # # NlaIV: # [GGNNCC] # 1 44 236 652 # # PleI: # [GAGTC] # 199 734 # # PpuMI: # [RGGWCCY] # 235 698 # # RsaI: # [GTAC] # 71 124 250 488 # # SanDI: # [GGGWCCC] # 235 698 # # SapI: # [GCTCTTC] # 36 897 # # SduI: # [GDGCHC] # 60 873 # # SetI: # [ASST] # 20 26 78 78 117 142 # 216 256 # # SmlI: # [CTYRAG] # 327 606 # # TatI: # [WGTACW] # 122 323 488 # # TauI: # [GCSGC] # 23 910 # # TfiI: # [GAWTC] # 18 27 151 186 551 # # TsoI: # [TARCCA] # 121 403 409 # # Tsp45I: # [GTSAC] # 251 682 # # Tsp4CI*: # [ACNGT] # 19 90 330 494 # # TspDTI: # [ATGAA] # 26 60 210 279 358 # # TspEI: # [AATT] # 19 22 22 28 47 50 # 50 106 117 149 156 167 # # TspGWI: # [ACGGA] # 227 706 # # TspRI: # [CASTGNN] # 48 306 579 # # VspI: # [ATTAAT] # 317 616 # # XhoII: # [RGATCY] # 220 713 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 933 # Reported_sequences: 1 # Reported_hitcount: 162 #---------------------------------------