Restriction Fragments for YCRWdelta11



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YCRWdelta11 on chromosome III from coordinates 169573 to 169888.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.10496.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 24 28 + BccI CCATC 32 33 . . 25 29 - FokI GGATG 11 15 . . 25 29 - BseGI GGATG 24 22 . . 32 36 + Hpy188I TCNGA 34 33 . . 43 46 + FatI CATG 42 46 . . 43 46 + NlaIII CATG 46 42 . . 43 46 + CviAII CATG 43 45 . . 41 45 - TspDTI ATGAA 31 29 . . 50 53 + MaeI CTAG 50 52 . . 64 67 + FatI CATG 63 67 . . 64 67 + NlaIII CATG 67 63 . . 64 67 + CviAII CATG 64 66 . . 68 71 + SetI ASST 71 67 . . 67 70 - MnlI CCTC 60 59 . . 76 80 + MboII GAAGA 88 87 . . 99 103 + Tsp4CI* ACNGT 101 100 . . 94 104 - Hin4I GAYNNNNNVTC 117 112 85 80 110 113 + SetI ASST 113 109 . . 127 130 + CviRI* TGCA 128 128 . . 154 158 + TspDTI ATGAA 169 167 . . 173 176 + SetI ASST 176 172 . . 171 175 - Hin4II* CCTTC 165 164 . . 202 206 + TfiI GAWTC 202 205 . . 202 206 + HinfI GANTC 202 205 . . 206 216 + BsiYI* CCNNNNNNNGG 212 209 . . 211 214 + MseI TTAA 211 213 . . 216 220 + TfiI GAWTC 216 219 . . 216 220 + HinfI GANTC 216 219 . . 227 234 + PspXI VCTCGAGB 228 232 . . 227 230 + MnlI CCTC 237 236 . . 228 233 + SmlI CTYRAG 228 232 . . 228 233 + XhoI CTCGAG 228 232 . . 228 233 + BmeT110I CYCGRG 229 231 . . 228 233 + AvaI CYCGRG 228 232 . . 229 232 + TaqI TCGA 229 231 . . 238 241 + TaiI ACGT 241 237 . . 238 241 + SetI ASST 241 237 . . 238 241 + MaeII ACGT 238 240 . . 242 245 + MaeI CTAG 242 244 . . 255 260 + SspI AATATT 257 257 . . 267 272 + AhaIII* TTTAAA 269 269 . . 268 271 + MseI TTAA 268 270 . . 275 279 + Tsp4CI* ACNGT 277 276 . . 276 282 + TspRI CASTGNN 282 273 . . 278 283 + Hpy166II GTNNAC 280 280 . . 280 285 + NlaIV GGNNCC 282 282 . . 280 284 + BmgT120I GGNCC 281 282 . . 280 284 + AvaII GGWCC 280 283 . . 280 284 + AsuI* GGNCC 280 283 . . 303 307 - HphI GGTGA 295 294 . . #--------------------------------------- # # # AhaIII*: # [TTTAAA] # 47 269 # # AsuI*: # [GGNCC] # 36 280 # # AvaI: # [CYCGRG] # 88 228 # # AvaII: # [GGWCC] # 36 280 # # BccI: # [CCATC] # 32 284 # # BmeT110I: # [CYCGRG] # 87 229 # # BmgT120I: # [GGNCC] # 35 281 # # BseGI: # [GGATG] # 24 292 # # BsiYI*: # [CCNNNNNNNGG] # 104 212 # # CviAII: # [CATG] # 21 43 252 # # CviRI*: # [TGCA] # 128 188 # # FatI: # [CATG] # 21 42 253 # # FokI: # [GGATG] # 11 305 # # Hin4I: # [GAYNNNNNVTC] # 32 85 199 # # Hin4II*: # [CCTTC] # 151 165 # # HinfI: # [GANTC] # 14 100 202 # # HphI: # [GGTGA] # 21 295 # # Hpy166II: # [GTNNAC] # 36 280 # # Hpy188I: # [TCNGA] # 34 282 # # MaeI: # [CTAG] # 50 74 192 # # MaeII: # [ACGT] # 78 238 # # MboII: # [GAAGA] # 88 228 # # MnlI: # [CCTC] # 60 79 177 # # MseI: # [TTAA] # 48 57 211 # # NlaIII: # [CATG] # 21 46 249 # # NlaIV: # [GGNNCC] # 34 282 # # PspXI: # [VCTCGAGB] # 88 228 # # SetI: # [ASST] # 42 63 65 71 75 # # SmlI: # [CTYRAG] # 88 228 # # SspI: # [AATATT] # 59 257 # # TaiI: # [ACGT] # 75 241 # # TaqI: # [TCGA] # 87 229 # # TfiI: # [GAWTC] # 14 100 202 # # Tsp4CI*: # [ACNGT] # 39 101 176 # # TspDTI: # [ATGAA] # 31 138 147 # # TspRI: # [CASTGNN] # 34 282 # # XhoI: # [CTCGAG] # 88 228 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 316 # Reported_sequences: 1 # Reported_hitcount: 50 #---------------------------------------