Restriction Fragments for YCRCtau1



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YCRCtau1 on chromosome III from coordinates 151055 to 150695.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.17172.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 18 + BalI TGGCCA 15 15 . . 13 18 + CfrI YGGCCR 13 17 . . 14 17 + CviJI RGCY 15 15 . . 14 17 + HaeIII GGCC 15 15 . . 18 23 + VspI ATTAAT 19 21 . . 19 22 + MseI TTAA 19 21 . . 59 68 + MslI CAYNNNNRTG 63 63 . . 65 74 + BsaBI GATNNNNATC 69 69 . . 67 72 + TatI WGTACW 67 71 . . 67 72 + Bsp1407I TGTACA 67 71 . . 68 71 + Csp6I GTAC 68 70 . . 68 71 + RsaI GTAC 69 69 . . 82 87 + BspLU11I* ACATGT 82 86 . . 82 87 + NspI RCATGY 86 82 . . 82 87 + AflIII ACRYGT 82 86 . . 83 92 + MslI CAYNNNNRTG 87 87 . . 83 86 + FatI CATG 82 86 . . 83 86 + NlaIII CATG 86 82 . . 83 86 + CviAII CATG 83 85 . . 104 108 - Hin4II* CCTTC 98 97 . . 109 114 + ApoI RAATTY 109 113 . . 110 113 + TspEI AATT 109 113 . . 113 117 - TspDTI ATGAA 103 101 . . 118 123 + NspI RCATGY 122 118 . . 119 122 + FatI CATG 118 122 . . 119 122 + NlaIII CATG 122 118 . . 119 122 + CviAII CATG 119 121 . . 121 124 + CviRI* TGCA 122 122 . . 129 132 + SetI ASST 132 128 . . 137 141 + TspDTI ATGAA 152 150 . . 145 155 + Hin4I GAYNNNNNVTC 136 131 168 163 148 151 + MseI TTAA 148 150 . . 151 156 + XhoII RGATCY 151 155 . . 151 156 + BglII AGATCT 151 155 . . 152 155 + DpnI GATC 153 153 . . 152 155 + BstKTI GATC 154 152 . . 152 155 + MboI GATC 151 155 . . 145 155 - Hin4I GAYNNNNNVTC 168 163 136 131 157 162 + VspI ATTAAT 158 160 . . 158 161 + MseI TTAA 158 160 . . 160 163 + TspEI AATT 159 163 . . 167 176 + MslI CAYNNNNRTG 171 171 . . 196 201 + VspI ATTAAT 197 199 . . 197 200 + MseI TTAA 197 199 . . 199 202 + TspEI AATT 198 202 . . 209 212 + MseI TTAA 209 211 . . 212 216 + Tsp4CI* ACNGT 214 213 . . 223 226 + SetI ASST 226 222 . . 223 226 + CviJI RGCY 224 224 . . 223 226 + AluI AGCT 224 224 . . 239 244 + SspI AATATT 241 241 . . 258 262 + TspDTI ATGAA 273 271 . . 263 267 + Tsp4CI* ACNGT 265 264 . . 270 275 + BtgZI GCGATG 285 289 . . 285 288 + MnlI CCTC 295 294 . . 300 303 + FatI CATG 299 303 . . 300 303 + NlaIII CATG 303 299 . . 300 303 + CviAII CATG 300 302 . . 302 313 + BdaI TGANNNNNNTCA 291 289 325 323 305 309 + MboII GAAGA 317 316 . . 308 312 + TfiI GAWTC 308 311 . . 308 312 + HinfI GANTC 308 311 . . 302 313 - BdaI TGANNNNNNTCA 325 323 291 289 310 314 - TspDTI ATGAA 300 298 . . 317 320 + MseI TTAA 317 319 . . 320 323 + SetI ASST 323 319 . . 321 325 + Hin4II* CCTTC 331 330 . . 323 327 - TspDTI ATGAA 313 311 . . 328 333 + VspI ATTAAT 329 331 . . 329 332 + MseI TTAA 329 331 . . 335 338 + Csp6I GTAC 335 337 . . 335 338 + RsaI GTAC 336 336 . . 337 340 + SetI ASST 340 336 . . 338 341 + MnlI CCTC 348 347 . . 339 344 + Ksp632I* CTCTTC 345 348 . . 340 344 - MboII GAAGA 332 331 . . 342 346 - TspDTI ATGAA 332 330 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 82 279 # # AluI: # [AGCT] # 137 224 # # ApoI: # [RAATTY] # 109 252 # # BalI: # [TGGCCA] # 15 346 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 36 291 # # BglII: # [AGATCT] # 151 210 # # BsaBI: # [GATNNNNATC] # 69 292 # # Bsp1407I: # [TGTACA] # 67 294 # # BspLU11I*: # [ACATGT] # 82 279 # # BstKTI: # [GATC] # 154 207 # # BtgZI: # [GCGATG] # 76 285 # # CfrI: # [YGGCCR] # 13 348 # # Csp6I: # [GTAC] # 26 68 267 # # CviAII: # [CATG] # 36 61 83 181 # # CviJI: # [RGCY] # 15 137 209 # # CviRI*: # [TGCA] # 122 239 # # DpnI: # [GATC] # 153 208 # # FatI: # [CATG] # 36 62 82 181 # # HaeIII: # [GGCC] # 15 346 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 136 193 # # Hin4II*: # [CCTTC] # 30 98 233 # # HinfI: # [GANTC] # 53 308 # # Ksp632I*: # [CTCTTC] # 16 345 # # MboI: # [GATC] # 151 210 # # MboII: # [GAAGA] # 15 29 317 # # MnlI: # [CCTC] # 13 53 295 # # MseI: # [TTAA] # 10 12 12 19 32 39 # 108 129 # # MslI: # [CAYNNNNRTG] # 24 63 84 190 # # NlaIII: # [CATG] # 36 58 86 181 # # NspI: # [RCATGY] # 36 86 239 # # RsaI: # [GTAC] # 25 69 267 # # SetI: # [ASST] # 17 21 94 97 132 # # SspI: # [AATATT] # 120 241 # # TatI: # [WGTACW] # 67 294 # # TfiI: # [GAWTC] # 53 308 # # Tsp4CI*: # [ACNGT] # 51 96 214 # # TspDTI: # [ATGAA] # 13 19 27 29 49 103 # 121 # # TspEI: # [AATT] # 39 50 109 163 # # VspI: # [ATTAAT] # 19 32 39 132 139 # # XhoII: # [RGATCY] # 151 210 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 361 # Reported_sequences: 1 # Reported_hitcount: 77 #---------------------------------------