Restriction Fragments for TUP1/YCR084C



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The currently selected gene/sequence is :

TUP1/YCR084C on chromosome III from coordinates 262452 to 260311.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20929.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 12 + Cac8I GCNNGC 9 9 . . 15 20 + AsuII TTCGAA 16 18 . . 16 19 + TaqI TCGA 16 18 . . 24 34 + MwoI GCNNNNNNNGC 30 27 . . 32 35 + SetI ASST 35 31 . . 32 35 + CviJI RGCY 33 33 . . 32 35 + AluI AGCT 33 33 . . 33 43 + MwoI GCNNNNNNNGC 39 36 . . 41 44 + SetI ASST 44 40 . . 41 44 + CviJI RGCY 42 42 . . 41 44 + AluI AGCT 42 42 . . 45 50 + Hpy178III* TCNNGA 46 48 . . 47 50 + TaqI TCGA 47 49 . . 49 53 - SfaNI GCATC 39 43 . . 53 57 + BccI CCATC 61 62 . . 56 60 + Hpy188I TCNGA 58 57 . . 76 80 + BsmAI GTCTC 81 85 . . 85 88 - MnlI CCTC 78 77 . . 94 97 + SetI ASST 97 93 . . 98 102 + Tsp4CI* ACNGT 100 99 . . 102 106 - MboII GAAGA 94 93 . . 101 106 - BbvII* GAAGAC 94 98 . . 133 137 + TspDTI ATGAA 148 146 . . 138 148 + BsiYI* CCNNNNNNNGG 144 141 . . 138 148 + PflMI CCANNNNNTGG 144 141 . . 139 144 + EcoP15I CAGCAG 169 171 . . 139 147 + AlwNI CAGNNNCTG 144 141 . . 141 145 + BlsI GCNGC 143 142 . . 141 145 + BisI GCNGC 142 143 . . 141 145 + BbvI GCAGC 153 157 . . 141 145 + TseI GCWGC 141 144 . . 142 147 + PvuII CAGCTG 144 144 . . 142 147 + NspBII* CMGCKG 144 144 . . 143 146 + SetI ASST 146 142 . . 143 146 + CviJI RGCY 144 144 . . 143 146 + AluI AGCT 144 144 . . 144 149 + Cac8I GCNNGC 146 146 . . 147 150 + CviJI RGCY 148 148 . . 149 153 + DdeI CTNAG 149 152 . . 149 153 - BspCNI CTCAG 141 139 . . 149 153 - BseMII CTCAG 140 138 . . 155 158 + CviRI* TGCA 156 156 . . 156 160 + BlsI GCNGC 158 157 . . 156 160 + BisI GCNGC 157 158 . . 156 160 + BbvI GCAGC 168 172 . . 156 160 + TseI GCWGC 156 159 . . 153 157 - SfaNI GCATC 143 147 . . 157 162 + EcoP15I CAGCAG 187 189 . . 172 176 + Tsp4CI* ACNGT 174 173 . . 175 180 + Hpy166II GTNNAC 177 177 . . 175 180 + AccI GTMKAC 176 178 . . 183 187 + BsrI ACTGG 188 186 . . 205 209 + TspDTI ATGAA 220 218 . . 207 219 + BarI GAAGNNNNNNTAC 199 194 231 226 207 211 - Hin4II* CCTTC 201 200 . . 211 215 + HgaI GACGC 220 225 . . 212 217 + MluI ACGCGT 212 216 . . 212 217 + AflIII ACRYGT 212 216 . . 213 216 + FnuDII* CGCG 214 214 . . 215 220 + SplI* CGTACG 215 219 . . 216 219 + Csp6I GTAC 216 218 . . 216 219 + RsaI GTAC 217 217 . . 220 224 + MboII GAAGA 232 231 . . 223 227 + MboII GAAGA 235 234 . . 228 231 + DpnI GATC 229 229 . . 228 231 + BstKTI GATC 230 228 . . 223 228 - Ksp632I* CTCTTC 218 221 . . 228 231 + MboI GATC 227 231 . . 244 247 + MaeI CTAG 244 246 . . 248 251 + CviJI RGCY 249 249 . . 250 255 + Eco57MI CTGRAG 271 269 . . 250 255 + GsuI CTGGAG 271 269 . . 264 268 + BccI CCATC 272 273 . . 260 264 - BsmAI GTCTC 254 258 . . 260 265 - Eco31I GGTCTC 254 258 . . 270 273 + TspEI AATT 269 273 . . 273 276 + CviRI* TGCA 274 274 . . 274 278 + SfaNI GCATC 283 287 . . 283 287 + Tsp4CI* ACNGT 285 284 . . 291 301 + MwoI GCNNNNNNNGC 297 294 . . 295 300 + NspBII* CMGCKG 297 297 . . 297 301 + TauI GCSGC 300 297 . . 297 307 + MwoI GCNNNNNNNGC 303 300 . . 297 301 + BlsI GCNGC 299 298 . . 297 301 + BisI GCNGC 298 299 . . 297 300 - AciI CCGC 297 299 . . 306 316 + MwoI GCNNNNNNNGC 312 309 . . 310 315 + EcoP15I CAGCAG 340 342 . . 312 316 + BlsI GCNGC 314 313 . . 312 316 + BisI GCNGC 313 314 . . 312 316 + BbvI GCAGC 324 328 . . 312 316 + TseI GCWGC 312 315 . . 320 323 + SetI ASST 323 319 . . 321 325 + BmgT120I GGNCC 322 323 . . 321 325 + AvaII GGWCC 321 324 . . 321 325 + AsuI* GGNCC 321 324 . . 325 330 + EcoP15I CAGCAG 355 357 . . 327 331 + BlsI GCNGC 329 328 . . 327 331 + BisI GCNGC 328 329 . . 327 331 + BbvI GCAGC 339 343 . . 327 331 + TseI GCWGC 327 330 . . 342 352 + MwoI GCNNNNNNNGC 348 345 . . 346 351 + EcoP15I CAGCAG 376 378 . . 348 352 + BlsI GCNGC 350 349 . . 348 352 + BisI GCNGC 349 350 . . 348 352 + BbvI GCAGC 360 364 . . 348 352 + TseI GCWGC 348 351 . . 349 354 + EcoP15I CAGCAG 379 381 . . 351 355 + BlsI GCNGC 353 352 . . 351 355 + BisI GCNGC 352 353 . . 351 355 + BbvI GCAGC 363 367 . . 351 355 + TseI GCWGC 351 354 . . 353 356 + SetI ASST 356 352 . . 353 356 + CviJI RGCY 354 354 . . 353 356 + AluI AGCT 354 354 . . 354 359 + Cac8I GCNNGC 356 356 . . 354 359 + BmtI GCTAGC 358 354 . . 354 359 + NheI GCTAGC 354 358 . . 355 358 + MaeI CTAG 355 357 . . 357 360 + CviJI RGCY 358 358 . . 358 362 + TauI GCSGC 361 358 . . 358 362 + BlsI GCNGC 360 359 . . 358 362 + BisI GCNGC 359 360 . . 359 364 + NspBII* CMGCKG 361 361 . . 359 362 + AciI CCGC 359 361 . . 361 371 + MwoI GCNNNNNNNGC 367 364 . . 361 365 + BlsI GCNGC 363 362 . . 361 365 + BisI GCNGC 362 363 . . 361 365 + TseI GCWGC 361 364 . . 363 366 + CviRI* TGCA 364 364 . . 364 368 + SfaNI GCATC 373 377 . . 361 365 - BbvI GCAGC 348 352 . . 369 372 + CviRI* TGCA 370 370 . . 370 374 + SfaNI GCATC 379 383 . . 379 383 - BsrI ACTGG 379 377 . . 384 389 + MstI* TGCGCA 386 386 . . 385 388 + Hin6I GCGC 385 387 . . 385 388 + GlaI GCGC 386 386 . . 385 388 + HhaI GCGC 387 385 . . 396 401 + Cfr10I RCCGGY 396 400 . . 397 400 + HpaII CCGG 397 399 . . 399 402 + CviJI RGCY 400 400 . . 410 415 + CfrI YGGCCR 410 414 . . 411 414 + CviJI RGCY 412 412 . . 411 414 + HaeIII GGCC 412 412 . . 414 425 + TstI CACNNNNNNTCC 405 400 437 432 415 425 + BsaXI ACNNNNNCTCC 405 402 435 432 416 419 + AciI CCGC 416 418 . . 418 428 + MwoI GCNNNNNNNGC 424 421 . . 425 430 + EcoP15I CAGCAG 455 457 . . 422 427 - Eco57MI CTGRAG 407 405 . . 422 427 - GsuI CTGGAG 407 405 . . 427 431 + BlsI GCNGC 429 428 . . 427 431 + BisI GCNGC 428 429 . . 427 431 + BbvI GCAGC 439 443 . . 427 431 + TseI GCWGC 427 430 . . 442 446 + BsrI ACTGG 447 445 . . 451 455 + BccI CCATC 459 460 . . 456 459 + CviJI RGCY 457 457 . . 456 459 + HaeIII GGCC 457 457 . . 458 462 + Hin4II* CCTTC 468 467 . . 465 470 + TatI WGTACW 465 469 . . 466 469 + Csp6I GTAC 466 468 . . 466 469 + RsaI GTAC 467 467 . . 463 467 - BsrI ACTGG 463 461 . . 462 467 - BfiI ACTGGG 457 456 . . 471 474 + SetI ASST 474 470 . . 471 474 + CviJI RGCY 472 472 . . 471 474 + AluI AGCT 472 472 . . 472 477 + Cac8I GCNNGC 474 474 . . 472 477 + BmtI GCTAGC 476 472 . . 472 477 + NheI GCTAGC 472 476 . . 473 476 + MaeI CTAG 473 475 . . 475 478 + CviJI RGCY 476 476 . . 476 480 - BceAI ACGGC 461 463 . . 493 496 + CviJI RGCY 494 494 . . 498 502 + Tsp4CI* ACNGT 500 499 . . 512 526 + XcmI CCANNNNNNNNNTGG 519 518 . . 530 533 + MnlI CCTC 540 539 . . 559 567 + AlwNI CAGNNNCTG 564 561 . . 566 569 + CviRI* TGCA 567 567 . . 567 577 + MwoI GCNNNNNNNGC 573 570 . . 571 576 + EcoP15I CAGCAG 601 603 . . 573 577 + BlsI GCNGC 575 574 . . 573 577 + BisI GCNGC 574 575 . . 573 577 + BbvI GCAGC 585 589 . . 573 577 + TseI GCWGC 573 576 . . 597 600 + SetI ASST 600 596 . . 598 601 + MnlI CCTC 608 607 . . 602 607 + SecI* CCNNGG 602 606 . . 603 607 + BssKI CCNGG 602 607 . . 603 607 + BseBI CCWGG 604 605 . . 603 607 + ScrFI CCNGG 604 605 . . 603 607 + EcoRII CCWGG 602 607 . . 605 608 + SetI ASST 608 604 . . 611 616 + DsaI* CCRYGG 611 615 . . 611 616 + SecI* CCNNGG 611 615 . . 610 614 - TspGWI ACGGA 600 598 . . 615 620 + NlaIV GGNNCC 617 617 . . 615 620 + HgiCI* GGYRCC 615 619 . . 626 630 + MaeIII GTNAC 625 630 . . 633 636 + CviJI RGCY 634 634 . . 635 639 + BccI CCATC 643 644 . . 641 645 + TspGWI ACGGA 656 654 . . 643 648 + XhoII RGATCY 643 647 . . 643 647 + BinI* GGATC 651 652 . . 644 647 + DpnI GATC 645 645 . . 644 647 + BstKTI GATC 646 644 . . 644 647 + MboI GATC 643 647 . . 661 665 - BsmAI GTCTC 655 659 . . 661 666 - Eco31I GGTCTC 655 659 . . 677 680 + MnlI CCTC 687 686 . . 687 692 + NlaIV GGNNCC 689 689 . . 687 692 + HgiCI* GGYRCC 687 691 . . 690 693 + SetI ASST 693 689 . . 694 698 + TfiI GAWTC 694 697 . . 694 698 + HinfI GANTC 694 697 . . 700 703 + TaiI ACGT 703 699 . . 700 703 + SetI ASST 703 699 . . 700 703 + MaeII ACGT 700 702 . . 717 722 + BetI* WCCGGW 717 721 . . 718 721 + HpaII CCGG 718 720 . . 733 736 + SetI ASST 736 732 . . 734 737 + MnlI CCTC 744 743 . . 736 739 + TaqI TCGA 736 738 . . 738 742 + MboII GAAGA 750 749 . . 748 758 + Hin4I GAYNNNNNVTC 739 734 771 766 751 756 + BetI* WCCGGW 751 755 . . 752 755 + HpaII CCGG 752 754 . . 754 759 + XhoII RGATCY 754 758 . . 754 759 + NlaIV GGNNCC 756 756 . . 754 759 + BamHI GGATCC 754 758 . . 754 758 + BinI* GGATC 762 763 . . 755 758 + DpnI GATC 756 756 . . 755 758 + BstKTI GATC 757 755 . . 755 758 + MboI GATC 754 758 . . 748 758 - Hin4I GAYNNNNNVTC 771 766 739 734 758 761 + AciI CCGC 758 760 . . 755 759 - BinI* GGATC 749 750 . . 760 770 + MwoI GCNNNNNNNGC 766 763 . . 760 770 + BglI GCCNNNNNGGC 766 763 . . 757 762 - EciI GGCGGA 747 745 . . 764 769 + DsaI* CCRYGG 764 768 . . 764 769 + SecI* CCNNGG 764 768 . . 766 770 + BceAI ACGGC 782 784 . . 767 772 + CfrI YGGCCR 767 771 . . 768 771 + CviJI RGCY 769 769 . . 768 771 + HaeIII GGCC 769 769 . . 782 785 + AciI CCGC 782 784 . . 804 807 + SetI ASST 807 803 . . 805 811 + SauI* CCTNAGG 806 809 . . 806 810 + DdeI CTNAG 806 809 . . 811 814 - MnlI CCTC 804 803 . . 814 818 + MboII GAAGA 826 825 . . 814 819 + BbvII* GAAGAC 821 825 . . 817 821 + HgaI GACGC 826 831 . . 817 822 + AcyI GRCGYC 818 820 . . 820 830 + MwoI GCNNNNNNNGC 826 823 . . 820 830 + BglI GCCNNNNNGGC 826 823 . . 824 829 + SecI* CCNNGG 824 828 . . 825 829 + BssKI CCNGG 824 829 . . 825 829 + ScrFI CCNGG 826 827 . . 825 829 + CauII* CCSGG 826 827 . . 826 829 + HpaII CCGG 826 828 . . 828 831 + CviJI RGCY 829 829 . . 830 833 + MaeI CTAG 830 832 . . 836 839 + CviRI* TGCA 837 837 . . 840 844 + BssKI CCNGG 839 844 . . 840 844 + BseBI CCWGG 841 842 . . 840 844 + ScrFI CCNGG 841 842 . . 840 844 + EcoRII CCWGG 839 844 . . 843 847 + BinI* GGATC 851 852 . . 844 847 + DpnI GATC 845 845 . . 844 847 + BstKTI GATC 846 844 . . 844 847 + MboI GATC 843 847 . . 852 856 + DdeI CTNAG 852 855 . . 856 860 - BslFI GGGAC 841 845 . . 861 866 + PsiI TTATAA 863 863 . . 888 891 + SetI ASST 891 887 . . 897 900 + SetI ASST 900 896 . . 909 914 + XhoII RGATCY 909 913 . . 909 913 + BinI* GGATC 917 918 . . 910 913 + DpnI GATC 911 911 . . 910 913 + BstKTI GATC 912 910 . . 910 913 + MboI GATC 909 913 . . 912 917 + XbaI TCTAGA 912 916 . . 912 917 + Hpy178III* TCNNGA 913 915 . . 913 916 + MaeI CTAG 913 915 . . 916 920 + TfiI GAWTC 916 919 . . 916 920 + HinfI GANTC 916 919 . . 916 926 + Hin4I GAYNNNNNVTC 907 902 939 934 921 925 - BsrI ACTGG 921 919 . . 920 925 - BfiI ACTGGG 915 914 . . 916 926 - Hin4I GAYNNNNNVTC 939 934 907 902 930 935 + Hpy178III* TCNNGA 931 933 . . 934 938 - SfaNI GCATC 924 928 . . 939 943 + Hpy188I TCNGA 941 940 . . 940 945 + Eco57MI CTGRAG 961 959 . . 940 945 + Eco57I CTGAAG 961 959 . . 942 946 + MboII GAAGA 954 953 . . 978 982 + BssKI CCNGG 977 982 . . 978 982 + ScrFI CCNGG 979 980 . . 978 982 + CauII* CCSGG 979 980 . . 979 982 + HpaII CCGG 979 981 . . 996 999 + TspEI AATT 995 999 . . 1001 1004 + TaiI ACGT 1004 1000 . . 1001 1004 + SetI ASST 1004 1000 . . 1001 1004 + MaeII ACGT 1001 1003 . . 1008 1012 + MaeIII GTNAC 1007 1012 . . 1023 1027 + BinI* GGATC 1031 1032 . . 1024 1027 + DpnI GATC 1025 1025 . . 1024 1027 + BstKTI GATC 1026 1024 . . 1024 1027 + MboI GATC 1023 1027 . . 1031 1036 - Eco57MI CTGRAG 1016 1014 . . 1031 1036 - Eco57I CTGAAG 1016 1014 . . 1058 1062 + MaeIII GTNAC 1057 1062 . . 1066 1070 + HphI GGTGA 1078 1077 . . 1063 1067 - BccI CCATC 1057 1058 . . 1074 1078 + DdeI CTNAG 1074 1077 . . 1076 1081 + TsoI TARCCA 1092 1090 . . 1077 1080 + CviJI RGCY 1078 1078 . . 1084 1087 + CviJI RGCY 1085 1085 . . 1085 1089 + BlsI GCNGC 1087 1086 . . 1085 1089 + BisI GCNGC 1086 1087 . . 1085 1089 + TseI GCWGC 1085 1088 . . 1087 1090 + CviRI* TGCA 1088 1088 . . 1085 1089 - BbvI GCAGC 1072 1076 . . 1100 1105 + SmlI CTYRAG 1100 1104 . . 1100 1105 - Bce83I* CTTGAG 1085 1083 . . 1111 1114 + FnuDII* CGCG 1112 1112 . . 1117 1121 + Hpy188I TCNGA 1119 1118 . . 1120 1124 - BccI CCATC 1114 1115 . . 1134 1137 + CviJI RGCY 1135 1135 . . 1134 1137 + HaeIII GGCC 1135 1135 . . 1134 1138 + BmgT120I GGNCC 1135 1136 . . 1134 1138 + AsuI* GGNCC 1134 1137 . . 1144 1148 + Hpy188I TCNGA 1146 1145 . . 1149 1160 + BcgI CGANNNNNNTGC 1138 1136 1172 1170 1150 1154 + TfiI GAWTC 1150 1153 . . 1150 1154 + HinfI GANTC 1150 1153 . . 1156 1160 + BlsI GCNGC 1158 1157 . . 1156 1160 + BisI GCNGC 1157 1158 . . 1156 1160 + TseI GCWGC 1156 1159 . . 1154 1159 - EcoP15I CAGCAG 1126 1128 . . 1156 1160 - BbvI GCAGC 1143 1147 . . 1164 1169 + TsoI TARCCA 1180 1178 . . 1167 1171 + BccI CCATC 1175 1176 . . 1170 1173 + TaqI TCGA 1170 1172 . . 1173 1178 + ApoI RAATTY 1173 1177 . . 1174 1177 + TspEI AATT 1173 1177 . . 1177 1180 + TaqI TCGA 1177 1179 . . 1179 1182 + DpnI GATC 1180 1180 . . 1179 1182 + BstKTI GATC 1181 1179 . . 1179 1182 + MboI GATC 1178 1182 . . 1182 1188 + TspRI CASTGNN 1188 1179 . . 1200 1205 + BtrI CACGTC 1202 1202 . . 1201 1204 + TaiI ACGT 1204 1200 . . 1201 1204 + SetI ASST 1204 1200 . . 1201 1204 + MaeII ACGT 1201 1203 . . 1203 1208 + Hpy166II GTNNAC 1205 1205 . . 1205 1210 + DsaI* CCRYGG 1205 1209 . . 1205 1210 + SecI* CCNNGG 1205 1209 . . 1207 1211 + TspGWI ACGGA 1222 1220 . . 1245 1248 + TspEI AATT 1244 1248 . . 1254 1263 + MslI CAYNNNNRTG 1258 1258 . . 1258 1263 + BtgZI GCGATG 1273 1277 . . 1270 1274 + BlsI GCNGC 1272 1271 . . 1270 1274 + BisI GCNGC 1271 1272 . . 1270 1274 + BbvI GCAGC 1282 1286 . . 1270 1274 + TseI GCWGC 1270 1273 . . 1271 1276 + EcoP15I CAGCAG 1301 1303 . . 1273 1283 + MwoI GCNNNNNNNGC 1279 1276 . . 1277 1280 + TspEI AATT 1276 1280 . . 1283 1294 + CspCI CAANNNNNGTGG 1271 1269 1306 1304 1298 1301 + SetI ASST 1301 1297 . . 1312 1316 - MboII GAAGA 1304 1303 . . 1311 1316 - BbvII* GAAGAC 1304 1308 . . 1318 1322 + BccI CCATC 1326 1327 . . 1324 1329 + MmeI TCCRAC 1349 1347 . . 1324 1328 + Hpy188I TCNGA 1326 1325 . . 1322 1326 - FokI GGATG 1308 1312 . . 1322 1326 - BseGI GGATG 1321 1319 . . 1337 1341 - TspGWI ACGGA 1327 1325 . . 1343 1349 + TspRI CASTGNN 1349 1340 . . 1356 1361 + Hpy178III* TCNNGA 1357 1359 . . 1355 1360 - Eco57MI CTGRAG 1340 1338 . . 1355 1360 - GsuI CTGGAG 1340 1338 . . 1360 1364 - BccI CCATC 1354 1355 . . 1366 1371 + ApoI RAATTY 1366 1370 . . 1367 1370 + TspEI AATT 1366 1370 . . 1375 1385 + MwoI GCNNNNNNNGC 1381 1378 . . 1375 1385 + BstAPI GCANNNNNTGC 1381 1378 . . 1379 1387 + AlwNI CAGNNNCTG 1384 1381 . . 1380 1383 + SetI ASST 1383 1379 . . 1385 1390 + Eco57MI CTGRAG 1406 1404 . . 1385 1390 + Eco57I CTGAAG 1406 1404 . . 1387 1391 + MboII GAAGA 1399 1398 . . 1387 1392 + BbvII* GAAGAC 1394 1398 . . 1403 1408 + ApoI RAATTY 1403 1407 . . 1404 1407 + TspEI AATT 1403 1407 . . 1437 1441 + TfiI GAWTC 1437 1440 . . 1437 1441 + HinfI GANTC 1437 1440 . . 1440 1444 - MboII GAAGA 1432 1431 . . 1447 1450 + CviJI RGCY 1448 1448 . . 1447 1450 + HaeIII GGCC 1448 1448 . . 1467 1471 - TspDTI ATGAA 1457 1455 . . 1484 1487 + MnlI CCTC 1494 1493 . . 1484 1490 + SauI* CCTNAGG 1485 1488 . . 1485 1489 + DdeI CTNAG 1485 1488 . . 1485 1489 + BspCNI CTCAG 1498 1496 . . 1485 1489 + BseMII CTCAG 1499 1497 . . 1488 1491 + SetI ASST 1491 1487 . . 1489 1493 + HphI GGTGA 1501 1500 . . 1490 1494 + Tsp45I GTSAC 1489 1494 . . 1490 1494 + MaeIII GTNAC 1489 1494 . . 1492 1503 + DrdI GACNNNNNNGTC 1498 1496 . . 1496 1499 + TspEI AATT 1495 1499 . . 1501 1505 + BsmAI GTCTC 1506 1510 . . 1504 1509 + BetI* WCCGGW 1504 1508 . . 1505 1508 + HpaII CCGG 1505 1507 . . 1513 1519 + BstEII GGTNACC 1513 1518 . . 1513 1517 + HphI GGTGA 1525 1524 . . 1514 1518 + Tsp45I GTSAC 1513 1518 . . 1514 1518 + MaeIII GTNAC 1513 1518 . . 1517 1521 + Tsp4CI* ACNGT 1519 1518 . . 1520 1523 + Csp6I GTAC 1520 1522 . . 1520 1523 + RsaI GTAC 1521 1521 . . 1522 1526 + Tsp4CI* ACNGT 1524 1523 . . 1535 1539 + BslFI GGGAC 1549 1553 . . 1541 1546 + SnaBI TACGTA 1543 1543 . . 1541 1546 + BsaAI YACGTR 1543 1543 . . 1542 1545 + TaiI ACGT 1545 1541 . . 1542 1545 + SetI ASST 1545 1541 . . 1542 1545 + MaeII ACGT 1542 1544 . . 1544 1547 + Csp6I GTAC 1544 1546 . . 1544 1547 + RsaI GTAC 1545 1545 . . 1549 1552 + CviJI RGCY 1550 1550 . . 1549 1552 + HaeIII GGCC 1550 1550 . . 1552 1558 + TspRI CASTGNN 1558 1549 . . 1551 1555 - BsrI ACTGG 1551 1549 . . 1557 1561 - TspDTI ATGAA 1547 1545 . . 1576 1580 + MboII GAAGA 1588 1587 . . 1579 1583 - BccI CCATC 1573 1574 . . 1585 1589 + MaeIII GTNAC 1584 1589 . . 1591 1595 + Tsp4CI* ACNGT 1593 1592 . . 1593 1597 + Hpy99I CGWCG 1597 1592 . . 1604 1612 + DraIII CACNNNGTG 1609 1606 . . 1605 1611 + SexAI ACCWGGT 1605 1610 . . 1606 1610 + BssKI CCNGG 1605 1610 . . 1606 1610 + BseBI CCWGG 1607 1608 . . 1606 1610 + ScrFI CCNGG 1607 1608 . . 1606 1610 + EcoRII CCWGG 1605 1610 . . 1606 1616 + BsiYI* CCNNNNNNNGG 1612 1609 . . 1606 1616 + PflMI CCANNNNNTGG 1612 1609 . . 1603 1607 - HphI GGTGA 1595 1594 . . 1608 1611 + SetI ASST 1611 1607 . . 1609 1613 + HphI GGTGA 1621 1620 . . 1612 1616 - BccI CCATC 1606 1607 . . 1627 1631 + BlsI GCNGC 1629 1628 . . 1627 1631 + BisI GCNGC 1628 1629 . . 1627 1631 + TseI GCWGC 1627 1630 . . 1623 1628 - BtgZI GCGATG 1608 1612 . . 1627 1631 - BbvI GCAGC 1614 1618 . . 1628 1633 - EcoP15I CAGCAG 1600 1602 . . 1638 1643 + XbaI TCTAGA 1638 1642 . . 1638 1643 + Hpy178III* TCNNGA 1639 1641 . . 1639 1642 + MaeI CTAG 1639 1641 . . 1642 1645 + DpnI GATC 1643 1643 . . 1642 1645 + BstKTI GATC 1644 1642 . . 1642 1645 + MboI GATC 1641 1645 . . 1663 1667 + TfiI GAWTC 1663 1666 . . 1663 1667 + HinfI GANTC 1663 1666 . . 1666 1670 + Hpy188I TCNGA 1668 1667 . . 1672 1677 + BetI* WCCGGW 1672 1676 . . 1673 1676 + HpaII CCGG 1673 1675 . . 1669 1673 - BsmAI GTCTC 1663 1667 . . 1669 1674 - Eco31I GGTCTC 1663 1667 . . 1676 1680 + TfiI GAWTC 1676 1679 . . 1676 1680 + HinfI GANTC 1676 1679 . . 1675 1686 - TstI CACNNNNNNTCC 1699 1694 1667 1662 1693 1696 + MaeI CTAG 1693 1695 . . 1696 1700 + TfiI GAWTC 1696 1699 . . 1696 1700 + HinfI GANTC 1696 1699 . . 1699 1703 + Hpy188I TCNGA 1701 1700 . . 1708 1712 + TfiI GAWTC 1708 1711 . . 1708 1712 + HinfI GANTC 1708 1711 . . 1711 1716 + BetI* WCCGGW 1711 1715 . . 1712 1715 + HpaII CCGG 1712 1714 . . 1715 1718 + Csp6I GTAC 1715 1717 . . 1715 1718 + RsaI GTAC 1716 1716 . . 1720 1723 + CviJI RGCY 1721 1721 . . 1720 1723 + HaeIII GGCC 1721 1721 . . 1729 1733 + HinfI GANTC 1729 1732 . . 1729 1733 - PleI GAGTC 1723 1724 . . 1729 1733 - MlyI GAGTC 1723 1723 . . 1748 1752 - MboII GAAGA 1740 1739 . . 1747 1752 - BbvII* GAAGAC 1740 1744 . . 1754 1757 + MaeI CTAG 1754 1756 . . 1759 1763 - BccI CCATC 1753 1754 . . 1774 1779 + BciVI GTATCC 1785 1784 . . 1778 1781 + HpaII CCGG 1778 1780 . . 1780 1783 + CviJI RGCY 1781 1781 . . 1792 1797 + XhoII RGATCY 1792 1796 . . 1792 1797 + BglII AGATCT 1792 1796 . . 1793 1796 + DpnI GATC 1794 1794 . . 1793 1796 + BstKTI GATC 1795 1793 . . 1793 1796 + MboI GATC 1792 1796 . . 1799 1802 + MseI TTAA 1799 1801 . . 1802 1805 + SetI ASST 1805 1801 . . 1802 1805 + CviJI RGCY 1803 1803 . . 1802 1805 + AluI AGCT 1803 1803 . . 1805 1810 + Hpy178III* TCNNGA 1806 1808 . . 1809 1814 + ApoI RAATTY 1809 1813 . . 1810 1813 + TspEI AATT 1809 1813 . . 1814 1817 + CviRI* TGCA 1815 1815 . . 1818 1823 + BsmI GAATGC 1824 1822 . . 1821 1824 + CviRI* TGCA 1822 1822 . . 1837 1841 + TfiI GAWTC 1837 1840 . . 1837 1841 + HinfI GANTC 1837 1840 . . 1839 1844 + AsuII TTCGAA 1840 1842 . . 1840 1843 + TaqI TCGA 1840 1842 . . 1849 1859 + BsiYI* CCNNNNNNNGG 1855 1852 . . 1851 1856 + ApoI RAATTY 1851 1855 . . 1852 1855 + TspEI AATT 1851 1855 . . 1856 1859 + HpaII CCGG 1856 1858 . . 1870 1874 + MaeIII GTNAC 1869 1874 . . 1872 1877 + SnaBI TACGTA 1874 1874 . . 1872 1877 + BsaAI YACGTR 1874 1874 . . 1873 1876 + TaiI ACGT 1876 1872 . . 1873 1876 + SetI ASST 1876 1872 . . 1873 1876 + MaeII ACGT 1873 1875 . . 1904 1909 + DsaI* CCRYGG 1904 1908 . . 1904 1909 + SecI* CCNNGG 1904 1908 . . 1903 1907 - TspGWI ACGGA 1893 1891 . . 1907 1912 + BalI TGGCCA 1909 1909 . . 1907 1912 + CfrI YGGCCR 1907 1911 . . 1908 1911 + CviJI RGCY 1909 1909 . . 1908 1911 + HaeIII GGCC 1909 1909 . . 1928 1933 + TatI WGTACW 1928 1932 . . 1929 1932 + Csp6I GTAC 1929 1931 . . 1929 1932 + RsaI GTAC 1930 1930 . . 1939 1944 + BetI* WCCGGW 1939 1943 . . 1940 1943 + HpaII CCGG 1940 1942 . . 1942 1947 + NlaIV GGNNCC 1944 1944 . . 1947 1958 + CspCI CAANNNNNGTGG 1935 1933 1970 1968 1951 1954 + DpnI GATC 1952 1952 . . 1951 1954 + BstKTI GATC 1953 1951 . . 1951 1954 + MboI GATC 1950 1954 . . 1962 1972 + BsiYI* CCNNNNNNNGG 1968 1965 . . 1972 1982 + Hin4I GAYNNNNNVTC 1963 1958 1995 1990 1972 1982 - Hin4I GAYNNNNNVTC 1995 1990 1963 1958 1982 1985 + HpaII CCGG 1982 1984 . . 1989 1993 - TspGWI ACGGA 1979 1977 . . 2003 2006 + CviRI* TGCA 2004 2004 . . 2007 2010 + SetI ASST 2010 2006 . . 2016 2021 + EcoRI GAATTC 2016 2020 . . 2016 2021 + ApoI RAATTY 2016 2020 . . 2017 2020 + TspEI AATT 2016 2020 . . 2034 2037 + CviJI RGCY 2035 2035 . . 2048 2053 + Eco31I GGTCTC 2054 2058 . . 2049 2053 + BsmAI GTCTC 2054 2058 . . 2053 2058 + NspBII* CMGCKG 2055 2055 . . 2053 2056 + AciI CCGC 2053 2055 . . 2058 2063 + NlaIV GGNNCC 2060 2060 . . 2059 2063 + BmgT120I GGNCC 2060 2061 . . 2059 2063 + AvaII GGWCC 2059 2062 . . 2059 2063 + AsuI* GGNCC 2059 2062 . . 2055 2060 - GsaI CCCAGC 2059 2055 . . 2055 2060 - BseYI CCCAGC 2055 2059 . . 2061 2066 + Hpy178III* TCNNGA 2062 2064 . . 2071 2076 + AclI AACGTT 2072 2074 . . 2072 2075 + TaiI ACGT 2075 2071 . . 2072 2075 + SetI ASST 2075 2071 . . 2072 2075 + MaeII ACGT 2072 2074 . . 2083 2087 + BsrI ACTGG 2088 2086 . . 2092 2096 + HphI GGTGA 2104 2103 . . 2090 2093 - AciI CCGC 2090 2092 . . 2130 2135 + HaeII RGCGCY 2134 2130 . . 2131 2134 + Hin6I GCGC 2131 2133 . . 2131 2134 + GlaI GCGC 2132 2132 . . 2131 2134 + HhaI GCGC 2133 2131 . . 2137 2140 + TspEI AATT 2136 2140 . . 2139 2142 + MseI TTAA 2139 2141 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 176 1966 # # AciI: # [CCGC] # 24 37 52 57 62 297 # 342 1271 # # AclI: # [AACGTT] # 70 2072 # # AcyI: # [GRCGYC] # 818 1324 # # AflIII: # [ACRYGT] # 212 1930 # # AluI: # [AGCT] # 9 33 102 118 210 339 # 1331 # # AlwNI: # [CAGNNNCTG] # 144 420 758 820 # # ApoI: # [RAATTY] # 37 42 126 165 193 406 # 1173 # # AsuI*: # [GGNCC] # 83 321 813 925 # # AsuII: # [TTCGAA] # 16 302 1824 # # AvaII: # [GGWCC] # 83 321 1738 # # BalI: # [TGGCCA] # 233 1909 # # BamHI: # [GGATCC] # 754 1388 # # BarI: # [GAAGNNNNNNTAC] # 32 199 1911 # # BbvI: # [GCAGC] # 3 9 12 15 15 71 # 76 139 146 153 156 332 # 487 528 # # BbvII*: # [GAAGAC] # 90 94 346 402 483 727 # # BccI: # [CCATC] # 28 33 57 61 61 147 # 151 184 187 211 219 389 # 414 # # Bce83I*: # [CTTGAG] # 1057 1085 # # BceAI: # [ACGGC] # 321 461 1360 # # BcgI: # [CGANNNNNNTGC] # 34 970 1138 # # BciVI: # [GTATCC] # 357 1785 # # BetI*: # [WCCGGW] # 34 39 168 203 228 717 # 753 # # BfiI: # [ACTGGG] # 457 458 1227 # # BglI: # [GCCNNNNNGGC] # 60 766 1316 # # BglII: # [AGATCT] # 350 1792 # # BinI*: # [GGATC] # 13 66 89 98 114 651 # 1111 # # BisI: # [GCNGC] # 3 3 7 15 15 15 # 21 66 71 114 141 142 # 146 357 512 514 # # BlsI: # [GCNGC] # 3 3 7 15 15 15 # 21 66 71 114 141 143 # 146 357 512 513 # # BmgT120I: # [GGNCC] # 82 322 813 925 # # BmtI: # [GCTAGC] # 118 358 1666 # # BsaAI: # [YACGTR] # 268 331 1543 # # BsaXI: # [ACNNNNNCTCC] # 30 405 1707 # # BseBI: # [CCWGG] # 237 535 604 766 # # BseGI: # [GGATG] # 821 1321 # # BseMII: # [CTCAG] # 140 643 1359 # # BseYI: # [CCCAGC] # 87 2055 # # BsiYI*: # [CCNNNNNNNGG] # 113 144 174 243 1468 # # BslFI: # [GGGAC] # 593 708 841 # # BsmAI: # [GTCTC] # 81 88 157 173 391 401 # 851 # # BsmI: # [GAATGC] # 318 1824 # # BspCNI: # [CTCAG] # 141 644 1357 # # BsrI: # [ACTGG] # 16 54 68 188 191 458 # 537 630 # # BssKI: # [CCNGG] # 15 138 222 537 602 628 # # BstAPI: # [GCANNNNNTGC] # 761 1381 # # BstEII: # [GGTNACC] # 629 1513 # # BstKTI: # [GATC] # 66 89 111 114 151 155 # 158 189 230 416 463 # # BtgZI: # [GCGATG] # 335 534 1273 # # BtrI: # [CACGTC] # 940 1202 # # Cac8I: # [GCNNGC] # 9 118 137 210 1668 # # CauII*: # [CCSGG] # 153 826 1163 # # Cfr10I: # [RCCGGY] # 396 1746 # # CfrI: # [YGGCCR] # 235 357 410 1140 # # Csp6I: # [GTAC] # 24 171 213 214 216 250 # 1054 # # CspCI: # [CAANNNNNGTGG] # 35 35 172 629 1271 # # CviJI: # [RGCY] # 4 4 4 7 9 12 # 15 18 22 33 42 45 # 50 60 60 101 102 102 # 105 106 107 126 135 140 # 171 249 313 # # CviRI*: # [TGCA] # 6 7 90 118 138 156 # 182 197 251 270 727 # # DdeI: # [CTNAG] # 46 149 222 411 657 657 # # DpnI: # [GATC] # 66 89 111 114 151 155 # 158 190 229 416 463 # # DraIII: # [CACNNNGTG] # 533 1609 # # DrdI: # [GACNNNNNNGTC] # 644 1498 # # DsaI*: # [CCRYGG] # 153 238 441 611 699 # # EciI: # [GGCGGA] # 747 1395 # # Eco31I: # [GGTCTC] # 88 254 391 401 1008 # # Eco57I: # [CTGAAG] # 55 390 736 961 # # Eco57MI: # [CTGRAG] # 55 66 136 271 324 554 # 736 # # EcoP15I: # [CAGCAG] # 3 15 18 21 76 146 # 153 169 175 299 525 542 # # EcoRI: # [GAATTC] # 126 2016 # # EcoRII: # [CCWGG] # 237 537 602 766 # # FnuDII*: # [CGCG] # 214 898 1030 # # FokI: # [GGATG] # 834 1308 # # GlaI: # [GCGC] # 10 386 1746 # # GsaI: # [CCCAGC] # 83 2059 # # GsuI: # [CTGGAG] # 136 271 802 933 # # HaeII: # [RGCGCY] # 8 2134 # # HaeIII: # [GGCC] # 45 102 171 188 233 312 # 313 366 412 # # HgaI: # [GACGC] # 220 606 1316 # # HgiCI*: # [GGYRCC] # 72 615 1455 # # HhaI: # [GCGC] # 9 387 1746 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 32 32 32 136 147 739 # 1024 # # Hin4II*: # [CCTTC] # 201 267 1674 # # Hin6I: # [GCGC] # 11 385 1746 # # HinfI: # [GANTC] # 12 13 20 21 108 222 # 226 234 287 305 694 # # HpaII: # [CCGG] # 34 39 42 66 74 78 # 84 153 160 168 321 397 # 526 # # HphI: # [GGTGA] # 24 26 38 70 423 483 # 1078 # # Hpy166II: # [GTNNAC] # 177 937 1028 # # Hpy178III*: # [TCNNGA] # 18 46 80 167 256 282 # 426 867 # # Hpy188I: # [TCNGA] # 27 33 58 178 180 342 # 441 883 # # Hpy99I: # [CGWCG] # 545 1597 # # Ksp632I*: # [CTCTTC] # 218 1924 # # MaeI: # [CTAG] # 54 61 83 111 118 244 # 357 388 726 # # MaeII: # [ACGT] # 70 199 200 301 331 341 # 700 # # MaeIII: # [GTNAC] # 24 50 71 273 285 382 # 432 625 # # MboI: # [GATC] # 66 89 111 114 151 155 # 158 192 227 416 463 # # MboII: # [GAAGA] # 3 33 76 94 95 128 # 138 152 156 350 402 515 # # MluI: # [ACGCGT] # 212 1930 # # MlyI: # [GAGTC] # 419 1723 # # MmeI: # [TCCRAC] # 793 1349 # # MnlI: # [CCTC] # 57 60 68 78 79 462 # 648 690 # # MseI: # [TTAA] # 3 340 1799 # # MslI: # [CAYNNNNRTG] # 884 1258 # # MstI*: # [TGCGCA] # 386 1756 # # MwoI: # [GCNNNNNNNGC] # 6 9 9 19 30 36 # 57 60 102 149 193 258 # 453 761 # # NheI: # [GCTAGC] # 118 354 1670 # # NlaIV: # [GGNNCC] # 67 72 82 116 617 1188 # # NspBII*: # [CMGCKG] # 64 87 144 153 1694 # # PflMI: # [CCANNNNNTGG] # 144 530 1468 # # PleI: # [GAGTC] # 419 1723 # # PsiI: # [TTATAA] # 863 1279 # # PvuII: # [CAGCTG] # 144 1998 # # RsaI: # [GTAC] # 24 171 212 214 217 250 # 1054 # # SauI*: # [CCTNAGG] # 657 679 806 # # ScrFI: # [CCNGG] # 15 138 222 535 604 628 # # SecI*: # [CCNNGG] # 9 60 153 238 381 602 # 699 # # SetI: # [ASST] # 8 9 9 10 33 33 # 35 49 53 54 65 66 # 67 71 71 82 84 85 # 97 104 108 118 126 134 # 177 194 200 # # SexAI: # [ACCWGGT] # 537 1605 # # SfaNI: # [GCATC] # 6 39 90 104 140 545 # 1218 # # SmlI: # [CTYRAG] # 1042 1100 # # SnaBI: # [TACGTA] # 268 331 1543 # # SplI*: # [CGTACG] # 215 1927 # # TaiI: # [ACGT] # 67 199 200 301 331 341 # 703 # # TaqI: # [TCGA] # 7 16 31 302 434 663 # 689 # # TatI: # [WGTACW] # 214 465 1463 # # TauI: # [GCSGC] # 61 300 1781 # # TfiI: # [GAWTC] # 12 13 20 129 222 226 # 234 287 305 694 # # TseI: # [GCWGC] # 3 10 15 15 21 66 # 71 114 141 146 156 357 # 512 515 # # TsoI: # [TARCCA] # 88 962 1092 # # Tsp45I: # [GTSAC] # 24 629 1489 # # Tsp4CI*: # [ACNGT] # 5 69 74 100 111 215 # 549 1019 # # TspDTI: # [ATGAA] # 72 90 148 595 1237 # # TspEI: # [AATT] # 6 32 37 42 71 90 # 92 120 165 178 269 314 # 726 # # TspGWI: # [ACGGA] # 56 86 105 163 566 566 # 600 # # TspRI: # [CASTGNN] # 161 209 584 1188 # # TstI: # [CACNNNNNNTCC] # 32 32 405 443 1230 # # XbaI: # [TCTAGA] # 504 726 912 # # XcmI: # [CCANNNNNNNNNTGG] # 519 1623 # # XhoII: # [RGATCY] # 111 155 350 643 883 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2142 # Reported_sequences: 1 # Reported_hitcount: 583 #---------------------------------------