Restriction Fragments for SED4/YCR067C



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The currently selected gene/sequence is :

SED4/YCR067C on chromosome III from coordinates 236322 to 233125.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.19659.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 18 + CviRI* TGCA 16 16 . . 25 30 + ZraI GACGTC 27 27 . . 25 30 + AcyI GRCGYC 26 28 . . 25 30 + AatII GACGTC 29 25 . . 26 29 + TaiI ACGT 29 25 . . 26 29 + SetI ASST 29 25 . . 26 29 + MaeII ACGT 26 28 . . 27 31 + Hpy99I CGWCG 31 26 . . 29 33 + Hpy188I TCNGA 31 30 . . 32 37 + EcoRV GATATC 34 34 . . 28 33 - MmeI TCCRAC 9 7 . . 36 40 + Hpy188I TCNGA 38 37 . . 37 47 + BsiYI* CCNNNNNNNGG 43 40 . . 52 57 + ApoI RAATTY 52 56 . . 53 56 + TspEI AATT 52 56 . . 56 61 + PsiI TTATAA 58 58 . . 62 66 + TspDTI ATGAA 77 75 . . 64 76 + BarI GAAGNNNNNNTAC 56 51 88 83 64 68 - Hin4II* CCTTC 58 57 . . 75 79 + BsrI ACTGG 80 78 . . 81 84 + CviJI RGCY 82 82 . . 94 97 + CviJI RGCY 95 95 . . 94 97 + HaeIII GGCC 95 95 . . 92 95 - MnlI CCTC 85 84 . . 96 100 - BsrI ACTGG 96 94 . . 103 106 + TspEI AATT 102 106 . . 107 110 + MnlI CCTC 117 116 . . 123 126 + TspEI AATT 122 126 . . 129 132 + SetI ASST 132 128 . . 129 132 + CviJI RGCY 130 130 . . 129 132 + AluI AGCT 130 130 . . 132 137 + AhaIII* TTTAAA 134 134 . . 133 136 + MseI TTAA 133 135 . . 136 146 + FalI AAGNNNNNCTT 127 122 159 154 137 140 + SetI ASST 140 136 . . 138 142 + HphI GGTGA 150 149 . . 139 144 + Hpy166II GTNNAC 141 141 . . 146 151 + Hpy178III* TCNNGA 147 149 . . 136 146 - FalI AAGNNNNNCTT 159 154 127 122 169 172 + SetI ASST 172 168 . . 167 171 - Hin4II* CCTTC 161 160 . . 174 179 + Hpy178III* TCNNGA 175 177 . . 180 183 + DpnI GATC 181 181 . . 180 183 + BstKTI GATC 182 180 . . 180 183 + MboI GATC 179 183 . . 190 194 + TfiI GAWTC 190 193 . . 190 194 + HinfI GANTC 190 193 . . 193 196 + TaqI TCGA 193 195 . . 205 208 + Hin6I GCGC 205 207 . . 205 208 + GlaI GCGC 206 206 . . 205 208 + HhaI GCGC 207 205 . . 202 206 - SfaNI GCATC 192 196 . . 209 220 + BstXI CCANNNNNNTGG 216 212 . . 213 217 - TspDTI ATGAA 203 201 . . 233 236 + SetI ASST 236 232 . . 237 241 + TfiI GAWTC 237 240 . . 237 241 + HinfI GANTC 237 240 . . 241 244 + MaeI CTAG 241 243 . . 250 253 + CviRI* TGCA 251 251 . . 251 256 + BsrDI GCAATG 258 256 . . 254 258 + TspDTI ATGAA 269 267 . . 258 262 + Tsp4CI* ACNGT 260 259 . . 263 266 + MseI TTAA 263 265 . . 268 272 + TfiI GAWTC 268 271 . . 268 272 + HinfI GANTC 268 271 . . 272 275 + MnlI CCTC 282 281 . . 275 280 + DsaI* CCRYGG 275 279 . . 275 280 + StyI CCWWGG 275 279 . . 275 280 + NcoI CCATGG 275 279 . . 275 280 + SecI* CCNNGG 275 279 . . 276 279 + FatI CATG 275 279 . . 276 279 + NlaIII CATG 279 275 . . 276 279 + CviAII CATG 276 278 . . 283 288 + AclI AACGTT 284 286 . . 284 287 + TaiI ACGT 287 283 . . 284 287 + SetI ASST 287 283 . . 284 287 + MaeII ACGT 284 286 . . 286 291 + Hpy166II GTNNAC 288 288 . . 286 291 + HpaI GTTAAC 288 288 . . 286 291 + HindII GTYRAC 288 288 . . 287 290 + MseI TTAA 287 289 . . 288 293 + TsoI TARCCA 304 302 . . 297 302 + SmlI CTYRAG 297 301 . . 297 302 + Bce83I* CTTGAG 318 316 . . 300 303 - MnlI CCTC 293 292 . . 331 336 + ApoI RAATTY 331 335 . . 332 335 + TspEI AATT 331 335 . . 338 343 + BalI TGGCCA 340 340 . . 338 343 + CfrI YGGCCR 338 342 . . 339 342 + CviJI RGCY 340 340 . . 339 342 + HaeIII GGCC 340 340 . . 341 345 - BsrI ACTGG 341 339 . . 362 367 + SfeI* CTRYAG 362 366 . . 370 374 + BccI CCATC 378 379 . . 371 375 - FokI GGATG 357 361 . . 371 375 - BseGI GGATG 370 368 . . 373 377 - TspGWI ACGGA 363 361 . . 383 387 + DdeI CTNAG 383 386 . . 391 396 + Hpy166II GTNNAC 393 393 . . 397 402 + VspI ATTAAT 398 400 . . 398 401 + MseI TTAA 398 400 . . 407 411 + BmgT120I GGNCC 408 409 . . 407 411 + AsuI* GGNCC 407 410 . . 408 411 + CviJI RGCY 409 409 . . 408 411 + HaeIII GGCC 409 409 . . 409 419 + MwoI GCNNNNNNNGC 415 412 . . 412 416 + Tsp4CI* ACNGT 414 413 . . 413 421 + AlwNI CAGNNNCTG 418 415 . . 417 420 + SetI ASST 420 416 . . 417 420 + CviJI RGCY 418 418 . . 417 420 + AluI AGCT 418 418 . . 418 428 + MwoI GCNNNNNNNGC 424 421 . . 418 422 + BlsI GCNGC 420 419 . . 418 422 + BisI GCNGC 419 420 . . 418 422 + TseI GCWGC 418 421 . . 420 423 + CviRI* TGCA 421 421 . . 422 427 + MfeI CAATTG 422 426 . . 418 422 - BbvI GCAGC 405 409 . . 423 426 + TspEI AATT 422 426 . . 431 434 + MnlI CCTC 441 440 . . 427 431 - HgaI GACGC 416 421 . . 433 437 + Hpy188I TCNGA 435 434 . . 434 439 + Eco57MI CTGRAG 455 453 . . 434 439 + Eco57I CTGAAG 455 453 . . 439 444 + SduI GDGCHC 443 439 . . 439 444 + BseSI GKGCMC 443 439 . . 444 450 + TspRI CASTGNN 450 441 . . 445 449 + Tsp4CI* ACNGT 447 446 . . 449 460 + BdaI TGANNNNNNTCA 438 436 472 470 450 459 + BsaBI GATNNNNATC 454 454 . . 455 459 + TfiI GAWTC 455 458 . . 455 459 + HinfI GANTC 455 458 . . 449 460 - BdaI TGANNNNNNTCA 472 470 438 436 465 469 + FauI CCCGC 473 475 . . 466 469 + AciI CCGC 466 468 . . 467 470 + FnuDII* CGCG 468 468 . . 472 475 + TspEI AATT 471 475 . . 478 482 + TspGWI ACGGA 493 491 . . 484 487 + TspEI AATT 483 487 . . 492 495 + TspEI AATT 491 495 . . 512 515 + MseI TTAA 512 514 . . 521 524 + CviRI* TGCA 522 522 . . 536 540 + Tsp4CI* ACNGT 538 537 . . 565 568 + TspEI AATT 564 568 . . 580 584 - HgaI GACGC 569 574 . . 590 594 + BslFI GGGAC 604 608 . . 593 597 + TspGWI ACGGA 608 606 . . 597 602 + ApoI RAATTY 597 601 . . 598 601 + TspEI AATT 597 601 . . 616 620 + TspGWI ACGGA 631 629 . . 625 634 + BsaBI GATNNNNATC 629 629 . . 652 655 + SetI ASST 655 651 . . 682 686 + BlsI GCNGC 684 683 . . 682 686 + BisI GCNGC 683 684 . . 682 686 + BbvI GCAGC 694 698 . . 682 686 + TseI GCWGC 682 685 . . 685 689 + SfaNI GCATC 694 698 . . 694 697 + TaqI TCGA 694 696 . . 685 696 - BcgI CGANNNNNNTGC 708 706 674 672 700 704 + Hpy188I TCNGA 702 701 . . 703 707 - BccI CCATC 697 698 . . 718 721 + MseI TTAA 718 720 . . 724 727 + MaeI CTAG 724 726 . . 726 729 + SetI ASST 729 725 . . 741 744 + SetI ASST 744 740 . . 746 749 + MseI TTAA 746 748 . . 749 754 + ApoI RAATTY 749 753 . . 750 753 + TspEI AATT 749 753 . . 753 758 + AhaIII* TTTAAA 755 755 . . 754 757 + MseI TTAA 754 756 . . 763 767 + SfaNI GCATC 772 776 . . 767 771 + Hin4II* CCTTC 777 776 . . 764 768 - FokI GGATG 750 754 . . 764 768 - BseGI GGATG 763 761 . . 771 774 + FatI CATG 770 774 . . 771 774 + NlaIII CATG 774 770 . . 771 774 + CviAII CATG 771 773 . . 769 773 - TspDTI ATGAA 759 757 . . 776 779 + TaqI TCGA 776 778 . . 795 800 + ClaI ATCGAT 796 798 . . 796 799 + TaqI TCGA 796 798 . . 801 805 + FokI GGATG 814 818 . . 801 805 + BseGI GGATG 807 805 . . 798 802 - BccI CCATC 792 793 . . 805 809 + BsmAI GTCTC 810 814 . . 828 831 + CviRI* TGCA 829 829 . . 857 861 + BccI CCATC 865 866 . . 875 878 + SetI ASST 878 874 . . 875 878 + CviJI RGCY 876 876 . . 875 878 + AluI AGCT 876 876 . . 880 885 + HindIII AAGCTT 880 884 . . 881 884 + SetI ASST 884 880 . . 881 884 + CviJI RGCY 882 882 . . 881 884 + AluI AGCT 882 882 . . 894 899 + NlaIV GGNNCC 896 896 . . 903 908 + TatI WGTACW 903 907 . . 904 907 + Csp6I GTAC 904 906 . . 904 907 + RsaI GTAC 905 905 . . 907 910 + FatI CATG 906 910 . . 907 910 + NlaIII CATG 910 906 . . 907 910 + CviAII CATG 907 909 . . 908 912 + TspDTI ATGAA 923 921 . . 910 914 + TfiI GAWTC 910 913 . . 910 914 + HinfI GANTC 910 913 . . 928 932 + MaeIII GTNAC 927 932 . . 931 941 + BsaXI ACNNNNNCTCC 921 918 951 948 939 944 + Hpy178III* TCNNGA 940 942 . . 943 947 + TfiI GAWTC 943 946 . . 943 947 + HinfI GANTC 943 946 . . 938 943 - Eco57MI CTGRAG 923 921 . . 938 943 - GsuI CTGGAG 923 921 . . 946 951 + XbaI TCTAGA 946 950 . . 946 951 + Hpy178III* TCNNGA 947 949 . . 947 950 + MaeI CTAG 947 949 . . 958 963 + Cac8I GCNNGC 960 960 . . 963 972 + MslI CAYNNNNRTG 967 967 . . 981 984 + DpnI GATC 982 982 . . 981 984 + BstKTI GATC 983 981 . . 981 984 + MboI GATC 980 984 . . 985 990 + AclI AACGTT 986 988 . . 986 989 + TaiI ACGT 989 985 . . 986 989 + SetI ASST 989 985 . . 986 989 + MaeII ACGT 986 988 . . 990 994 + Hpy188I TCNGA 992 991 . . 991 996 + Eco57MI CTGRAG 1012 1010 . . 991 996 + Eco57I CTGAAG 1012 1010 . . 994 999 + HindIII AAGCTT 994 998 . . 995 998 + SetI ASST 998 994 . . 995 998 + CviJI RGCY 996 996 . . 995 998 + AluI AGCT 996 996 . . 1000 1005 + BetI* WCCGGW 1000 1004 . . 1001 1004 + HpaII CCGG 1001 1003 . . 1004 1007 + Csp6I GTAC 1004 1006 . . 1004 1007 + RsaI GTAC 1005 1005 . . 1017 1020 + TspEI AATT 1016 1020 . . 1027 1032 + SspI AATATT 1029 1029 . . 1035 1044 + XmnI GAANNNNTTC 1039 1039 . . 1036 1041 + ApoI RAATTY 1036 1040 . . 1037 1040 + TspEI AATT 1036 1040 . . 1043 1048 + Hpy178III* TCNNGA 1044 1046 . . 1047 1052 + ApoI RAATTY 1047 1051 . . 1048 1051 + TspEI AATT 1047 1051 . . 1071 1074 + CviJI RGCY 1072 1072 . . 1072 1077 + Cac8I GCNNGC 1074 1074 . . 1075 1080 + HaeII RGCGCY 1079 1075 . . 1076 1079 + Hin6I GCGC 1076 1078 . . 1076 1079 + GlaI GCGC 1077 1077 . . 1076 1079 + HhaI GCGC 1078 1076 . . 1087 1090 + MaeI CTAG 1087 1089 . . 1090 1093 + CviJI RGCY 1091 1091 . . 1105 1110 + Hpy166II GTNNAC 1107 1107 . . 1105 1110 + SduI GDGCHC 1109 1105 . . 1105 1110 + BseSI GKGCMC 1109 1105 . . 1105 1110 + ApaLI GTGCAC 1105 1109 . . 1105 1110 + HgiAI* GWGCWC 1109 1105 . . 1106 1109 + CviRI* TGCA 1107 1107 . . 1109 1113 + BceAI ACGGC 1125 1127 . . 1111 1114 + CviJI RGCY 1112 1112 . . 1125 1130 + NspI RCATGY 1129 1125 . . 1126 1135 + MslI CAYNNNNRTG 1130 1130 . . 1126 1129 + FatI CATG 1125 1129 . . 1126 1129 + NlaIII CATG 1129 1125 . . 1126 1129 + CviAII CATG 1126 1128 . . 1131 1136 + Hpy178III* TCNNGA 1132 1134 . . 1131 1136 + BspHI TCATGA 1131 1135 . . 1132 1135 + FatI CATG 1131 1135 . . 1132 1135 + NlaIII CATG 1135 1131 . . 1132 1135 + CviAII CATG 1132 1134 . . 1147 1150 + TaqI TCGA 1147 1149 . . 1150 1155 + ApoI RAATTY 1150 1154 . . 1151 1154 + TspEI AATT 1150 1154 . . 1174 1184 + Hin4I GAYNNNNNVTC 1165 1160 1197 1192 1176 1181 + StyI CCWWGG 1176 1180 . . 1176 1181 + SecI* CCNNGG 1176 1180 . . 1180 1185 + XhoII RGATCY 1180 1184 . . 1180 1184 + BinI* GGATC 1188 1189 . . 1181 1184 + DpnI GATC 1182 1182 . . 1181 1184 + BstKTI GATC 1183 1181 . . 1181 1184 + MboI GATC 1180 1184 . . 1174 1184 - Hin4I GAYNNNNNVTC 1197 1192 1165 1160 1183 1187 - MboII GAAGA 1175 1174 . . 1186 1190 - MboII GAAGA 1178 1177 . . 1201 1206 + VspI ATTAAT 1202 1204 . . 1202 1205 + MseI TTAA 1202 1204 . . 1217 1220 - MnlI CCTC 1210 1209 . . 1232 1237 + BsgI GTGCAG 1253 1251 . . 1233 1236 + CviRI* TGCA 1234 1234 . . 1237 1242 + EcoRV GATATC 1239 1239 . . 1246 1251 + HaeII RGCGCY 1250 1246 . . 1246 1251 + Eco47III AGCGCT 1248 1248 . . 1247 1250 + Hin6I GCGC 1247 1249 . . 1247 1250 + GlaI GCGC 1248 1248 . . 1247 1250 + HhaI GCGC 1249 1247 . . 1250 1255 + SfeI* CTRYAG 1250 1254 . . 1261 1266 + Cac8I GCNNGC 1263 1263 . . 1261 1266 + BmtI GCTAGC 1265 1261 . . 1261 1266 + NheI GCTAGC 1261 1265 . . 1262 1265 + MaeI CTAG 1262 1264 . . 1273 1277 - BsmAI GTCTC 1267 1271 . . 1279 1284 + ApoI RAATTY 1279 1283 . . 1280 1283 + TspEI AATT 1279 1283 . . 1286 1289 + MnlI CCTC 1296 1295 . . 1298 1301 + MaeI CTAG 1298 1300 . . 1321 1325 + MboII GAAGA 1333 1332 . . 1333 1338 + ApoI RAATTY 1333 1337 . . 1334 1337 + TspEI AATT 1333 1337 . . 1344 1349 + EcoRV GATATC 1346 1346 . . 1355 1360 + PvuII CAGCTG 1357 1357 . . 1355 1360 + NspBII* CMGCKG 1357 1357 . . 1356 1359 + SetI ASST 1359 1355 . . 1356 1359 + CviJI RGCY 1357 1357 . . 1356 1359 + AluI AGCT 1357 1357 . . 1360 1364 + TfiI GAWTC 1360 1363 . . 1360 1364 + HinfI GANTC 1360 1363 . . 1367 1370 + TspEI AATT 1366 1370 . . 1376 1381 + PstI CTGCAG 1380 1376 . . 1376 1381 + SfeI* CTRYAG 1376 1380 . . 1377 1380 + CviRI* TGCA 1378 1378 . . 1381 1386 + EcoRV GATATC 1383 1383 . . 1395 1398 + SetI ASST 1398 1394 . . 1395 1398 + CviJI RGCY 1396 1396 . . 1395 1398 + AluI AGCT 1396 1396 . . 1387 1400 - AjuI GAANNNNNNNTTGG 1412 1407 1380 1375 1399 1403 - MboII GAAGA 1391 1390 . . 1402 1406 - MboII GAAGA 1394 1393 . . 1405 1409 - MboII GAAGA 1397 1396 . . 1408 1412 - MboII GAAGA 1400 1399 . . 1411 1415 - MboII GAAGA 1403 1402 . . 1414 1418 - MboII GAAGA 1406 1405 . . 1417 1421 - MboII GAAGA 1409 1408 . . 1420 1424 - MboII GAAGA 1412 1411 . . 1423 1427 - MboII GAAGA 1415 1414 . . 1433 1437 + TspDTI ATGAA 1448 1446 . . 1435 1439 + MboII GAAGA 1447 1446 . . 1435 1440 - Ksp632I* CTCTTC 1430 1433 . . 1444 1448 + Tsp45I GTSAC 1443 1448 . . 1444 1448 + MaeIII GTNAC 1443 1448 . . 1451 1455 + TspDTI ATGAA 1466 1464 . . 1457 1462 + MfeI CAATTG 1457 1461 . . 1458 1461 + TspEI AATT 1457 1461 . . 1465 1469 - MboII GAAGA 1457 1456 . . 1464 1469 - BbvII* GAAGAC 1457 1461 . . 1456 1469 - AjuI GAANNNNNNNTTGG 1481 1476 1449 1444 1470 1473 + SetI ASST 1473 1469 . . 1468 1472 - HphI GGTGA 1460 1459 . . 1474 1477 + SetI ASST 1477 1473 . . 1475 1478 + MnlI CCTC 1485 1484 . . 1477 1481 + Hpy188I TCNGA 1479 1478 . . 1482 1485 + TspEI AATT 1481 1485 . . 1490 1493 + CviJI RGCY 1491 1491 . . 1491 1495 + TauI GCSGC 1494 1491 . . 1491 1495 + BlsI GCNGC 1493 1492 . . 1491 1495 + BisI GCNGC 1492 1493 . . 1492 1495 + AciI CCGC 1492 1494 . . 1494 1499 + Cac8I GCNNGC 1496 1496 . . 1494 1499 + BmtI GCTAGC 1498 1494 . . 1494 1499 + NheI GCTAGC 1494 1498 . . 1495 1498 + MaeI CTAG 1495 1497 . . 1497 1500 + CviJI RGCY 1498 1498 . . 1499 1502 + MnlI CCTC 1509 1508 . . 1505 1510 + SfeI* CTRYAG 1505 1509 . . 1516 1521 + SspI AATATT 1518 1518 . . 1523 1526 + TaqI TCGA 1523 1525 . . 1531 1535 + BccI CCATC 1539 1540 . . 1532 1537 - BtgZI GCGATG 1517 1521 . . 1541 1547 + TspRI CASTGNN 1547 1538 . . 1545 1550 + EcoRI GAATTC 1545 1549 . . 1545 1550 + ApoI RAATTY 1545 1549 . . 1546 1549 + TspEI AATT 1545 1549 . . 1549 1553 + Hpy188I TCNGA 1551 1550 . . 1552 1556 + PleI GAGTC 1560 1561 . . 1552 1556 + MlyI GAGTC 1561 1561 . . 1552 1556 + HinfI GANTC 1552 1555 . . 1555 1558 + TaqI TCGA 1555 1557 . . 1561 1571 + Hin4I GAYNNNNNVTC 1552 1547 1584 1579 1571 1574 + MnlI CCTC 1581 1580 . . 1561 1571 - Hin4I GAYNNNNNVTC 1584 1579 1552 1547 1576 1580 - MboII GAAGA 1568 1567 . . 1581 1586 + ApoI RAATTY 1581 1585 . . 1582 1585 + TspEI AATT 1581 1585 . . 1590 1601 + TstI CACNNNNNNTCC 1581 1576 1613 1608 1599 1604 + Hpy178III* TCNNGA 1600 1602 . . 1600 1606 + Bpu10I CCTNAGC 1601 1604 . . 1601 1605 + DdeI CTNAG 1601 1604 . . 1604 1607 + CviJI RGCY 1605 1605 . . 1601 1605 - BspCNI CTCAG 1593 1591 . . 1601 1605 - BseMII CTCAG 1592 1590 . . 1608 1612 + HinfI GANTC 1608 1611 . . 1611 1616 + Hpy178III* TCNNGA 1612 1614 . . 1608 1612 - PleI GAGTC 1602 1603 . . 1608 1612 - MlyI GAGTC 1602 1602 . . 1621 1626 + BseRI GAGGAG 1636 1634 . . 1621 1624 - MnlI CCTC 1614 1613 . . 1628 1631 + TspEI AATT 1627 1631 . . 1623 1633 - BsaXI ACNNNNNCTCC 1645 1642 1615 1612 1634 1637 + MnlI CCTC 1644 1643 . . 1624 1634 - Hin4I GAYNNNNNVTC 1647 1642 1615 1610 1639 1642 + MseI TTAA 1639 1641 . . 1654 1658 + Hpy188I TCNGA 1656 1655 . . 1698 1703 + SmlI CTYRAG 1698 1702 . . 1698 1703 + AflII CTTAAG 1698 1702 . . 1699 1702 + MseI TTAA 1699 1701 . . 1702 1707 + TatI WGTACW 1702 1706 . . 1702 1707 + ScaI AGTACT 1704 1704 . . 1703 1706 + Csp6I GTAC 1703 1705 . . 1703 1706 + RsaI GTAC 1704 1704 . . 1708 1712 + TfiI GAWTC 1708 1711 . . 1708 1712 + HinfI GANTC 1708 1711 . . 1713 1716 + SetI ASST 1716 1712 . . 1714 1718 + Hin4II* CCTTC 1724 1723 . . 1711 1715 - HphI GGTGA 1703 1702 . . 1727 1732 + NspI RCATGY 1731 1727 . . 1728 1731 + FatI CATG 1727 1731 . . 1728 1731 + NlaIII CATG 1731 1727 . . 1728 1731 + CviAII CATG 1728 1730 . . 1731 1735 - BceAI ACGGC 1716 1718 . . 1735 1739 - MboII GAAGA 1727 1726 . . 1734 1739 - BbvII* GAAGAC 1727 1731 . . 1737 1741 - TspDTI ATGAA 1727 1725 . . 1741 1745 - MboII GAAGA 1733 1732 . . 1743 1747 - TspDTI ATGAA 1733 1731 . . 1747 1751 - MboII GAAGA 1739 1738 . . 1749 1753 - TspDTI ATGAA 1739 1737 . . 1763 1766 + MnlI CCTC 1773 1772 . . 1770 1775 + Hpy166II GTNNAC 1772 1772 . . 1770 1775 + HpaI GTTAAC 1772 1772 . . 1770 1775 + HindII GTYRAC 1772 1772 . . 1771 1774 + MseI TTAA 1771 1773 . . 1778 1781 + MaeI CTAG 1778 1780 . . 1780 1783 + CviJI RGCY 1781 1781 . . 1787 1792 + SfeI* CTRYAG 1787 1791 . . 1805 1808 + Csp6I GTAC 1805 1807 . . 1805 1808 + RsaI GTAC 1806 1806 . . 1808 1811 + AciI CCGC 1808 1810 . . 1809 1812 + FnuDII* CGCG 1810 1810 . . 1818 1824 + BstEII GGTNACC 1818 1823 . . 1819 1823 + MaeIII GTNAC 1818 1823 . . 1816 1819 - AciI CCGC 1816 1818 . . 1851 1854 + CviRI* TGCA 1852 1852 . . 1852 1856 + BlsI GCNGC 1854 1853 . . 1852 1856 + BisI GCNGC 1853 1854 . . 1852 1856 + BbvI GCAGC 1864 1868 . . 1852 1856 + TseI GCWGC 1852 1855 . . 1849 1853 - SfaNI GCATC 1839 1843 . . 1854 1857 + CviJI RGCY 1855 1855 . . 1869 1874 + XbaI TCTAGA 1869 1873 . . 1869 1874 + Hpy178III* TCNNGA 1870 1872 . . 1870 1873 + MaeI CTAG 1870 1872 . . 1884 1889 + Cfr10I RCCGGY 1884 1888 . . 1885 1888 + HpaII CCGG 1885 1887 . . 1888 1892 + SfaNI GCATC 1897 1901 . . 1889 1893 - FokI GGATG 1875 1879 . . 1889 1893 - BseGI GGATG 1888 1886 . . 1893 1898 + BsiI* CACGAG 1893 1897 . . 1905 1910 + PsiI TTATAA 1907 1907 . . 1923 1929 + TspRI CASTGNN 1929 1920 . . 1925 1930 + Eco57MI CTGRAG 1946 1944 . . 1925 1930 + Eco57I CTGAAG 1946 1944 . . 1941 1944 + TspEI AATT 1940 1944 . . 1959 1962 + SetI ASST 1962 1958 . . 1959 1964 + BspMI ACCTGC 1968 1972 . . 1962 1965 + CviRI* TGCA 1963 1963 . . 1963 1967 + SfaNI GCATC 1972 1976 . . 1966 1970 + Hpy188I TCNGA 1968 1967 . . 1969 1973 + TfiI GAWTC 1969 1972 . . 1969 1973 + HinfI GANTC 1969 1972 . . 1977 1980 + CviRI* TGCA 1978 1978 . . 1983 1986 + CviJI RGCY 1984 1984 . . 1984 1989 + NmeAIII GCCGAG 2010 2008 . . 1996 2000 + MaeIII GTNAC 1995 2000 . . 2005 2009 - MboII GAAGA 1997 1996 . . 2008 2012 - MboII GAAGA 2000 1999 . . 2019 2024 + Hpy166II GTNNAC 2021 2021 . . 2019 2024 + HindII GTYRAC 2021 2021 . . 2022 2026 + HgaI GACGC 2031 2036 . . 2022 2027 + AcyI GRCGYC 2023 2025 . . 2027 2041 + XcmI CCANNNNNNNNNTGG 2034 2033 . . 2044 2048 - MboII GAAGA 2036 2035 . . 2052 2057 + Esp3I CGTCTC 2058 2062 . . 2053 2057 + BsmAI GTCTC 2058 2062 . . 2056 2060 + Hpy188I TCNGA 2058 2057 . . 2061 2064 + TspEI AATT 2060 2064 . . 2065 2068 + DpnI GATC 2066 2066 . . 2065 2068 + BstKTI GATC 2067 2065 . . 2065 2068 + MboI GATC 2064 2068 . . 2065 2069 - BinI* GGATC 2059 2060 . . 2074 2078 + SfaNI GCATC 2083 2087 . . 2077 2081 + Hpy188I TCNGA 2079 2078 . . 2081 2084 + TspEI AATT 2080 2084 . . 2103 2106 + SetI ASST 2106 2102 . . 2109 2114 - BsmI GAATGC 2109 2107 . . 2120 2125 + SfeI* CTRYAG 2120 2124 . . 2125 2129 + SfaNI GCATC 2134 2138 . . 2128 2132 + Hpy188I TCNGA 2130 2129 . . 2126 2130 - FokI GGATG 2112 2116 . . 2126 2130 - BseGI GGATG 2125 2123 . . 2131 2136 + ApoI RAATTY 2131 2135 . . 2132 2135 + TspEI AATT 2131 2135 . . 2137 2141 + HinfI GANTC 2137 2140 . . 2139 2143 + DdeI CTNAG 2139 2142 . . 2139 2143 + BspCNI CTCAG 2152 2150 . . 2139 2143 + BseMII CTCAG 2153 2151 . . 2137 2141 - PleI GAGTC 2131 2132 . . 2137 2141 - MlyI GAGTC 2131 2131 . . 2146 2150 - HgaI GACGC 2135 2140 . . 2152 2155 + TspEI AATT 2151 2155 . . 2157 2160 + DpnI GATC 2158 2158 . . 2157 2160 + BstKTI GATC 2159 2157 . . 2157 2160 + MboI GATC 2156 2160 . . 2157 2161 - BinI* GGATC 2151 2152 . . 2165 2169 + TspDTI ATGAA 2180 2178 . . 2167 2171 + MboII GAAGA 2179 2178 . . 2171 2176 + ApoI RAATTY 2171 2175 . . 2172 2175 + TspEI AATT 2171 2175 . . 2178 2183 + Hpy166II GTNNAC 2180 2180 . . 2178 2183 + HindII GTYRAC 2180 2180 . . 2188 2192 + SfaNI GCATC 2197 2201 . . 2192 2197 + SecI* CCNNGG 2192 2196 . . 2189 2193 - FokI GGATG 2175 2179 . . 2193 2197 + BssKI CCNGG 2192 2197 . . 2189 2193 - BseGI GGATG 2188 2186 . . 2193 2197 + BseBI CCWGG 2194 2195 . . 2193 2197 + ScrFI CCNGG 2194 2195 . . 2193 2197 + EcoRII CCWGG 2192 2197 . . 2197 2201 + HinfI GANTC 2197 2200 . . 2197 2201 - PleI GAGTC 2191 2192 . . 2197 2201 - MlyI GAGTC 2191 2191 . . 2207 2210 + MnlI CCTC 2217 2216 . . 2211 2215 + SfaNI GCATC 2220 2224 . . 2214 2218 + Hpy188I TCNGA 2216 2215 . . 2212 2216 - FokI GGATG 2198 2202 . . 2212 2216 - BseGI GGATG 2211 2209 . . 2218 2223 + TatI WGTACW 2218 2222 . . 2219 2222 + Csp6I GTAC 2219 2221 . . 2219 2222 + RsaI GTAC 2220 2220 . . 2227 2231 + Hpy188I TCNGA 2229 2228 . . 2230 2234 + TfiI GAWTC 2230 2233 . . 2230 2234 + HinfI GANTC 2230 2233 . . 2232 2236 - TspDTI ATGAA 2222 2220 . . 2242 2245 + SetI ASST 2245 2241 . . 2243 2246 + MnlI CCTC 2253 2252 . . 2248 2251 + CviJI RGCY 2249 2249 . . 2253 2257 - BsrI ACTGG 2253 2251 . . 2269 2272 + TaqI TCGA 2269 2271 . . 2272 2276 + Tsp4CI* ACNGT 2274 2273 . . 2275 2279 + MaeIII GTNAC 2274 2279 . . 2281 2285 - MboII GAAGA 2273 2272 . . 2296 2300 + Hpy188I TCNGA 2298 2297 . . 2299 2303 + TfiI GAWTC 2299 2302 . . 2299 2303 + HinfI GANTC 2299 2302 . . 2308 2312 + Hin4II* CCTTC 2318 2317 . . 2316 2320 + DdeI CTNAG 2316 2319 . . 2316 2320 + BspCNI CTCAG 2329 2327 . . 2316 2320 + BseMII CTCAG 2330 2328 . . 2317 2321 + Hpy188I TCNGA 2319 2318 . . 2320 2324 + TfiI GAWTC 2320 2323 . . 2320 2324 + HinfI GANTC 2320 2323 . . 2335 2340 + ApoI RAATTY 2335 2339 . . 2336 2339 + TspEI AATT 2335 2339 . . 2339 2343 - TspDTI ATGAA 2329 2327 . . 2345 2349 + BccI CCATC 2353 2354 . . 2350 2354 + Hpy188I TCNGA 2352 2351 . . 2355 2358 + SetI ASST 2358 2354 . . 2361 2365 - TspDTI ATGAA 2351 2349 . . 2367 2370 + SetI ASST 2370 2366 . . 2367 2370 + CviJI RGCY 2368 2368 . . 2367 2370 + AluI AGCT 2368 2368 . . 2380 2384 + BceAI ACGGC 2396 2398 . . 2377 2381 - BsmAI GTCTC 2371 2375 . . 2377 2382 - Esp3I CGTCTC 2371 2375 . . 2387 2390 + MaeI CTAG 2387 2389 . . 2389 2392 + SetI ASST 2392 2388 . . 2389 2392 + CviJI RGCY 2390 2390 . . 2389 2392 + AluI AGCT 2390 2390 . . 2412 2416 + MboII GAAGA 2424 2423 . . 2412 2417 + BbvII* GAAGAC 2419 2423 . . 2419 2422 + TaqI TCGA 2419 2421 . . 2419 2424 + Hpy178III* TCNNGA 2420 2422 . . 2427 2430 + TspEI AATT 2426 2430 . . 2441 2446 + SecI* CCNNGG 2441 2445 . . 2446 2450 + TfiI GAWTC 2446 2449 . . 2443 2446 - MnlI CCTC 2436 2435 . . 2446 2450 + HinfI GANTC 2446 2449 . . 2449 2453 + Hpy188I TCNGA 2451 2450 . . 2460 2466 + TspRI CASTGNN 2466 2457 . . 2457 2462 - Eco57MI CTGRAG 2442 2440 . . 2457 2462 - GsuI CTGGAG 2442 2440 . . 2459 2463 - BsrI ACTGG 2459 2457 . . 2476 2479 + TspEI AATT 2475 2479 . . 2479 2483 + Hpy188I TCNGA 2481 2480 . . 2487 2496 + MslI CAYNNNNRTG 2491 2491 . . 2487 2496 + OliI CACNNNNGTG 2491 2491 . . 2489 2500 + BstXI CCANNNNNNTGG 2496 2492 . . 2490 2496 + TspRI CASTGNN 2496 2487 . . 2489 2493 - BsrI ACTGG 2489 2487 . . 2499 2502 + CviJI RGCY 2500 2500 . . 2506 2509 + TspEI AATT 2505 2509 . . 2511 2516 + NlaIV GGNNCC 2513 2513 . . 2516 2530 + XcmI CCANNNNNNNNNTGG 2523 2522 . . 2518 2523 + SpeI ACTAGT 2518 2522 . . 2519 2522 + MaeI CTAG 2519 2521 . . 2529 2532 + CviJI RGCY 2530 2530 . . 2536 2539 + TspEI AATT 2535 2539 . . 2539 2543 + Hpy188I TCNGA 2541 2540 . . 2541 2546 + NlaIV GGNNCC 2543 2543 . . 2546 2560 + XcmI CCANNNNNNNNNTGG 2553 2552 . . 2548 2553 + SpeI ACTAGT 2548 2552 . . 2549 2552 + MaeI CTAG 2549 2551 . . 2559 2562 + CviJI RGCY 2560 2560 . . 2564 2568 + Tsp4CI* ACNGT 2566 2565 . . 2569 2573 + Hpy188I TCNGA 2571 2570 . . 2571 2576 + NlaIV GGNNCC 2573 2573 . . 2578 2583 + SpeI ACTAGT 2578 2582 . . 2579 2582 + MaeI CTAG 2579 2581 . . 2589 2595 + TspRI CASTGNN 2595 2586 . . 2593 2597 - SfaNI GCATC 2583 2587 . . 2598 2603 + TatI WGTACW 2598 2602 . . 2598 2603 + Bsp1407I TGTACA 2598 2602 . . 2599 2602 + Csp6I GTAC 2599 2601 . . 2599 2602 + RsaI GTAC 2600 2600 . . 2607 2610 + SetI ASST 2610 2606 . . 2609 2613 + DdeI CTNAG 2609 2612 . . 2605 2609 - HphI GGTGA 2597 2596 . . 2616 2626 - Hin4I GAYNNNNNVTC 2639 2634 2607 2602 2629 2633 - MboII GAAGA 2621 2620 . . 2638 2642 + Hpy188I TCNGA 2640 2639 . . 2641 2645 + TfiI GAWTC 2641 2644 . . 2641 2645 + HinfI GANTC 2641 2644 . . 2650 2655 + SspI AATATT 2652 2652 . . 2645 2656 - CspCI CAANNNNNGTGG 2669 2667 2634 2632 2659 2663 + BslFI GGGAC 2673 2677 . . 2656 2660 - Hin4II* CCTTC 2650 2649 . . 2671 2675 + SfaNI GCATC 2680 2684 . . 2679 2682 + DpnI GATC 2680 2680 . . 2679 2682 + BstKTI GATC 2681 2679 . . 2679 2682 + MboI GATC 2678 2682 . . 2702 2707 + MfeI CAATTG 2702 2706 . . 2690 2702 - BarI GAAGNNNNNNTAC 2714 2709 2682 2677 2703 2706 + TspEI AATT 2702 2706 . . 2707 2712 + HaeII RGCGCY 2711 2707 . . 2708 2711 + Hin6I GCGC 2708 2710 . . 2708 2711 + GlaI GCGC 2709 2709 . . 2708 2711 + HhaI GCGC 2710 2708 . . 2716 2720 + Hpy188I TCNGA 2718 2717 . . 2741 2746 + DsaI* CCRYGG 2741 2745 . . 2741 2746 + SecI* CCNNGG 2741 2745 . . 2740 2744 - TspGWI ACGGA 2730 2728 . . 2750 2753 + Hin6I GCGC 2750 2752 . . 2750 2753 + GlaI GCGC 2751 2751 . . 2750 2760 + MwoI GCNNNNNNNGC 2756 2753 . . 2750 2753 + HhaI GCGC 2752 2750 . . 2751 2754 + FnuDII* CGCG 2752 2752 . . 2745 2755 - BsaXI ACNNNNNCTCC 2767 2764 2737 2734 2746 2756 - Hin4I GAYNNNNNVTC 2769 2764 2737 2732 2752 2756 - HgaI GACGC 2741 2746 . . 2761 2765 + Tsp4CI* ACNGT 2763 2762 . . 2766 2769 + SetI ASST 2769 2765 . . 2766 2769 + CviJI RGCY 2767 2767 . . 2766 2769 + AluI AGCT 2767 2767 . . 2767 2773 + EspI* GCTNAGC 2768 2771 . . 2768 2772 + DdeI CTNAG 2768 2771 . . 2768 2772 + BspCNI CTCAG 2781 2779 . . 2768 2772 + BseMII CTCAG 2782 2780 . . 2771 2774 + CviJI RGCY 2772 2772 . . 2773 2783 + BsiYI* CCNNNNNNNGG 2779 2776 . . 2775 2779 - SfaNI GCATC 2765 2769 . . 2779 2783 + BssKI CCNGG 2778 2783 . . 2779 2783 + BseBI CCWGG 2780 2781 . . 2779 2783 + ScrFI CCNGG 2780 2781 . . 2779 2783 + EcoRII CCWGG 2778 2783 . . 2781 2784 + SetI ASST 2784 2780 . . 2785 2789 + MaeIII GTNAC 2784 2789 . . 2786 2791 + TsoI TARCCA 2802 2800 . . 2800 2804 + BccI CCATC 2808 2809 . . 2815 2818 + CviJI RGCY 2816 2816 . . 2818 2821 + MnlI CCTC 2828 2827 . . 2834 2837 + MaeI CTAG 2834 2836 . . 2840 2845 + MfeI CAATTG 2840 2844 . . 2841 2844 + TspEI AATT 2840 2844 . . 2846 2851 + EcoT22I ATGCAT 2850 2846 . . 2847 2850 + CviRI* TGCA 2848 2848 . . 2848 2852 + SfaNI GCATC 2857 2861 . . 2845 2849 - SfaNI GCATC 2835 2839 . . 2851 2856 + Hpy178III* TCNNGA 2852 2854 . . 2859 2864 + TatI WGTACW 2859 2863 . . 2859 2864 + Bsp1407I TGTACA 2859 2863 . . 2860 2863 + Csp6I GTAC 2860 2862 . . 2860 2863 + RsaI GTAC 2861 2861 . . 2883 2888 + TatI WGTACW 2883 2887 . . 2884 2887 + Csp6I GTAC 2884 2886 . . 2884 2887 + RsaI GTAC 2885 2885 . . 2886 2890 + Tsp4CI* ACNGT 2888 2887 . . 2899 2903 + TfiI GAWTC 2899 2902 . . 2899 2903 + HinfI GANTC 2899 2902 . . 2901 2905 - TspDTI ATGAA 2891 2889 . . 2905 2909 + Hpy188I TCNGA 2907 2906 . . 2906 2911 + Eco57MI CTGRAG 2927 2925 . . 2906 2911 + Eco57I CTGAAG 2927 2925 . . 2920 2923 + Csp6I GTAC 2920 2922 . . 2920 2923 + RsaI GTAC 2921 2921 . . 2959 2964 + Hpy178III* TCNNGA 2960 2962 . . 2963 2968 + BsmI GAATGC 2969 2967 . . 2972 2976 + Hpy99I CGWCG 2976 2971 . . 2972 2983 + BcgI CGANNNNNNTGC 2961 2959 2995 2993 2974 2978 + TspGWI ACGGA 2989 2987 . . 2981 2984 + CviRI* TGCA 2982 2982 . . 2982 2987 + Cac8I GCNNGC 2984 2984 . . 2986 2990 + SfaNI GCATC 2995 2999 . . 2993 2996 + Csp6I GTAC 2993 2995 . . 2993 2996 + RsaI GTAC 2994 2994 . . 2995 2999 + Tsp4CI* ACNGT 2997 2996 . . 3004 3008 + MaeIII GTNAC 3003 3008 . . 3010 3014 + MboII GAAGA 3022 3021 . . 3010 3015 + BbvII* GAAGAC 3017 3021 . . 3013 3018 + ZraI GACGTC 3015 3015 . . 3013 3018 + AcyI GRCGYC 3014 3016 . . 3013 3018 + AatII GACGTC 3017 3013 . . 3014 3017 + TaiI ACGT 3017 3013 . . 3014 3017 + SetI ASST 3017 3013 . . 3014 3017 + MaeII ACGT 3014 3016 . . 3015 3020 + Esp3I CGTCTC 3021 3025 . . 3016 3020 + BsmAI GTCTC 3021 3025 . . 3026 3029 + MaeI CTAG 3026 3028 . . 3047 3050 + MseI TTAA 3047 3049 . . 3050 3053 + TaiI ACGT 3053 3049 . . 3050 3053 + SetI ASST 3053 3049 . . 3050 3053 + MaeII ACGT 3050 3052 . . 3057 3060 + DpnI GATC 3058 3058 . . 3057 3060 + BstKTI GATC 3059 3057 . . 3057 3060 + MboI GATC 3056 3060 . . 3077 3083 + TspRI CASTGNN 3083 3074 . . 3076 3080 - BsrI ACTGG 3076 3074 . . 3081 3084 + CviJI RGCY 3082 3082 . . 3081 3084 + HaeIII GGCC 3082 3082 . . 3081 3086 + NlaIV GGNNCC 3083 3083 . . 3081 3085 + BmgT120I GGNCC 3082 3083 . . 3081 3085 + AsuI* GGNCC 3081 3084 . . 3085 3088 + MnlI CCTC 3095 3094 . . 3088 3091 + AciI CCGC 3088 3090 . . 3090 3101 + AlfI GCANNNNNNTGC 3079 3077 3113 3111 3095 3100 + MfeI CAATTG 3095 3099 . . 3096 3099 + TspEI AATT 3095 3099 . . 3099 3102 + CviRI* TGCA 3100 3100 . . 3100 3104 + BlsI GCNGC 3102 3101 . . 3100 3104 + BisI GCNGC 3101 3102 . . 3100 3104 + BbvI GCAGC 3112 3116 . . 3100 3104 + TseI GCWGC 3100 3103 . . 3090 3101 - AlfI GCANNNNNNTGC 3113 3111 3079 3077 3105 3108 + SetI ASST 3108 3104 . . 3118 3121 + TspEI AATT 3117 3121 . . 3120 3125 + AsuII TTCGAA 3121 3123 . . 3121 3124 + TaqI TCGA 3121 3123 . . 3136 3141 + Hpy166II GTNNAC 3138 3138 . . 3136 3141 + HindII GTYRAC 3138 3138 . . 3141 3152 + BcgI CGANNNNNNTGC 3130 3128 3164 3162 3150 3153 + CviRI* TGCA 3151 3151 . . 3160 3165 + NlaIV GGNNCC 3162 3162 . . 3163 3167 + Tsp4CI* ACNGT 3165 3164 . . 3166 3171 + Hpy166II GTNNAC 3168 3168 . . 3175 3180 + Cac8I GCNNGC 3177 3177 . . 3178 3181 + CviJI RGCY 3179 3179 . . 3178 3181 + HaeIII GGCC 3179 3179 . . 3180 3184 + Hin4II* CCTTC 3190 3189 . . 3183 3188 + Hpy178III* TCNNGA 3184 3186 . . 3183 3188 + BspHI TCATGA 3183 3187 . . 3184 3187 + FatI CATG 3183 3187 . . 3184 3187 + NlaIII CATG 3187 3183 . . 3184 3187 + CviAII CATG 3184 3186 . . 3182 3186 - TspDTI ATGAA 3172 3170 . . 3191 3194 + TspEI AATT 3190 3194 . . #--------------------------------------- # # # AatII: # [GACGTC] # 29 181 2988 # # AciI: # [CCGC] # 8 110 316 466 1026 1272 # # AclI: # [AACGTT] # 284 702 2212 # # AcyI: # [GRCGYC] # 26 184 991 1997 # # AflII: # [CTTAAG] # 1500 1698 # # AhaIII*: # [TTTAAA] # 134 621 2443 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 37 1380 1717 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 85 3079 # # AluI: # [AGCT] # 6 22 39 114 130 288 # 361 377 431 458 972 # # AlwNI: # [CAGNNNCTG] # 418 2780 # # ApaLI: # [GTGCAC] # 1105 2093 # # ApoI: # [RAATTY] # 11 36 40 52 54 103 # 129 152 164 212 266 279 # 287 550 863 # # AsuI*: # [GGNCC] # 117 407 2674 # # AsuII: # [TTCGAA] # 77 3121 # # BalI: # [TGGCCA] # 340 2858 # # BarI: # [GAAGNNNNNNTAC] # 32 32 56 484 2594 # # BbvI: # [GCAGC] # 86 289 405 1170 1248 # # BbvII*: # [GAAGAC] # 181 270 598 692 1457 # # BccI: # [CCATC] # 73 95 319 378 390 455 # 674 814 # # Bce83I*: # [CTTGAG] # 318 2880 # # BceAI: # [ACGGC] # 591 680 802 1125 # # BcgI: # [CGANNNNNNTGC] # 34 34 34 34 135 674 # 2253 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 438 2726 # # BetI*: # [WCCGGW] # 1000 2198 # # BinI*: # [GGATC] # 92 871 1047 1188 # # BisI: # [GCNGC] # 97 264 361 419 809 1248 # # BlsI: # [GCNGC] # 96 264 361 420 809 1248 # # BmgT120I: # [GGNCC] # 116 408 2674 # # BmtI: # [GCTAGC] # 233 1265 1700 # # Bpu10I: # [CCTNAGC] # 1597 1601 # # BsaBI: # [GATNNNNATC] # 175 454 2569 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 431 664 921 # 1092 # # BseBI: # [CCWGG] # 418 586 2194 # # BseGI: # [GGATG] # 23 44 63 237 370 393 # 987 1081 # # BseMII: # [CTCAG] # 177 416 452 561 1592 # # BseRI: # [GAGGAG] # 1562 1636 # # BseSI: # [GKGCMC] # 443 666 2089 # # BsgI: # [GTGCAG] # 1253 1945 # # BsiI*: # [CACGAG] # 1305 1893 # # BsiYI*: # [CCNNNNNNNGG] # 43 419 2736 # # BslFI: # [GGGAC] # 525 604 2069 # # BsmAI: # [GTCTC] # 177 313 457 650 791 810 # # BsmI: # [GAATGC] # 229 860 2109 # # Bsp1407I: # [TGTACA] # 261 339 2598 # # BspCNI: # [CTCAG] # 177 417 452 559 1593 # # BspHI: # [TCATGA] # 15 1131 2052 # # BspMI: # [ACCTGC] # 1230 1968 # # BsrDI: # [GCAATG] # 258 2940 # # BsrI: # [ACTGG] # 16 30 80 122 206 245 # 587 1912 # # BssKI: # [CCNGG] # 420 586 2192 # # BstEII: # [GGTNACC] # 1380 1818 # # BstKTI: # [GATC] # 92 139 182 200 378 522 # 801 884 # # BstXI: # [CCANNNNNNTGG] # 216 702 2280 # # BtgZI: # [GCGATG] # 1517 1681 # # Cac8I: # [GCNNGC] # 21 114 189 193 233 960 # 1488 # # Cfr10I: # [RCCGGY] # 1314 1884 # # CfrI: # [YGGCCR] # 338 2860 # # ClaI: # [ATCGAT] # 796 2402 # # Csp6I: # [GTAC] # 24 36 73 100 102 205 # 261 380 414 699 904 # # CspCI: # [CAANNNNNGTGG] # 35 529 2634 # # CviAII: # [CATG] # 6 14 136 219 276 495 # 596 1456 # # CviJI: # [RGCY] # 5 6 7 9 13 19 # 19 21 22 30 30 35 # 39 44 69 74 76 82 # 95 97 107 110 114 119 # 129 176 207 210 245 265 # 266 458 # # CviRI*: # [TGCA] # 15 16 47 51 101 111 # 118 127 134 144 170 235 # 278 307 474 870 # # DdeI: # [CTNAG] # 159 177 293 383 430 538 # 1218 # # DpnI: # [GATC] # 92 140 181 200 378 522 # 801 884 # # DsaI*: # [CCRYGG] # 275 457 2466 # # Eco47III: # [AGCGCT] # 1248 1950 # # Eco57I: # [CTGAAG] # 271 455 557 934 981 # # Eco57MI: # [CTGRAG] # 89 271 455 468 485 496 # 934 # # EcoRI: # [GAATTC] # 1545 1653 # # EcoRII: # [CCWGG] # 420 586 2192 # # EcoRV: # [GATATC] # 34 37 107 1205 1815 # # EcoT22I: # [ATGCAT] # 348 2850 # # Esp3I: # [CGTCTC] # 177 313 650 2058 # # EspI*: # [GCTNAGC] # 430 2768 # # FalI: # [AAGNNNNNCTT] # 0 0 32 127 3039 # # FatI: # [CATG] # 6 15 136 219 275 495 # 596 1456 # # FauI: # [CCCGC] # 473 2725 # # FnuDII*: # [CGCG] # 446 468 942 1342 # # FokI: # [GGATG] # 23 63 64 237 357 393 # 1000 1061 # # GlaI: # [GCGC] # 42 171 206 447 871 1461 # # GsuI: # [CTGGAG] # 756 923 1519 # # HaeII: # [RGCGCY] # 171 487 1079 1461 # # HaeIII: # [GGCC] # 19 69 95 97 245 2673 # # HgaI: # [GACGC] # 104 153 416 457 606 1462 # # HgiAI*: # [GWGCWC] # 1109 2089 # # HhaI: # [GCGC] # 42 171 207 446 871 1461 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 31 32 # 32 32 32 32 98 355 # 429 960 1165 # # Hin4II*: # [CCTTC] # 8 58 103 332 540 594 # 616 947 # # Hin6I: # [GCGC] # 42 171 205 448 871 1461 # # HindII: # [GTYRAC] # 60 159 249 288 958 1484 # # HindIII: # [AAGCTT] # 114 880 2204 # # HinfI: # [GANTC] # 21 31 33 33 47 56 # 60 69 100 126 168 187 # 190 192 195 258 261 299 # 417 455 # # HpaI: # [GTTAAC] # 288 1426 1484 # # HpaII: # [CCGG] # 884 1001 1313 # # HphI: # [GGTGA] # 150 243 601 894 1310 # # Hpy166II: # [GTNNAC] # 30 30 105 141 147 159 # 249 665 714 958 # # Hpy178III*: # [TCNNGA] # 7 12 14 28 88 97 # 108 147 224 258 432 468 # 550 765 # # Hpy188I: # [TCNGA] # 7 13 21 21 30 30 # 31 33 51 60 69 69 # 72 78 86 90 99 105 # 189 267 290 291 312 397 # 487 # # Hpy99I: # [CGWCG] # 31 222 2945 # # Ksp632I*: # [CTCTTC] # 1430 1768 # # MaeI: # [CTAG] # 30 30 36 92 132 140 # 172 175 192 197 223 241 # 255 283 483 517 # # MaeII: # [ACGT] # 26 36 148 258 702 2028 # # MaeIII: # [GTNAC] # 177 195 219 279 375 510 # 516 927 # # MboI: # [GATC] # 92 142 179 200 378 522 # 801 884 # # MboII: # [GAAGA] # 3 3 3 3 3 3 # 3 3 3 3 6 6 # 10 32 36 58 94 111 # 143 151 155 159 176 197 # 258 401 1175 # # MfeI: # [CAATTG] # 103 138 255 422 1035 1245 # # MlyI: # [GAGTC] # 41 60 529 1007 1561 # # MmeI: # [TCCRAC] # 9 3189 # # MnlI: # [CCTC] # 11 24 30 32 33 36 # 72 85 86 103 129 148 # 165 183 189 267 392 444 # 769 # # MseI: # [TTAA] # 8 24 28 60 72 111 # 114 130 133 151 206 437 # 448 1276 # # MslI: # [CAYNNNNRTG] # 163 707 967 1361 # # MwoI: # [GCNNNNNNNGC] # 9 415 442 2332 # # NcoI: # [CCATGG] # 275 2923 # # NheI: # [GCTAGC] # 233 1261 1704 # # NlaIII: # [CATG] # 6 11 136 219 279 495 # 596 1456 # # NlaIV: # [GGNNCC] # 30 30 36 79 510 896 # 1617 # # NmeAIII: # [GCCGAG] # 1188 2010 # # NspBII*: # [CMGCKG] # 1357 1841 # # NspI: # [RCATGY] # 602 1129 1467 # # OliI: # [CACNNNNGTG] # 707 2491 # # PleI: # [GAGTC] # 42 60 529 1007 1560 # # PsiI: # [TTATAA] # 58 1291 1849 # # PstI: # [CTGCAG] # 1380 1818 # # PvuII: # [CAGCTG] # 1357 1841 # # RsaI: # [GTAC] # 24 36 73 100 102 204 # 261 380 414 699 905 # # ScaI: # [AGTACT] # 1494 1704 # # ScrFI: # [CCNGG] # 418 586 2194 # # SduI: # [GDGCHC] # 443 666 2089 # # SecI*: # [CCNNGG] # 249 275 300 457 901 1016 # # SetI: # [ASST] # 4 6 8 9 12 15 # 15 22 29 32 36 39 # 51 55 64 74 75 90 # 103 105 113 133 134 139 # 144 159 218 233 235 239 # 246 361 # # SfaNI: # [GCATC] # 22 23 51 58 63 70 # 75 78 85 97 111 138 # 192 203 363 502 1067 # # SfeI*: # [CTRYAG] # 126 129 282 333 362 888 # 1078 # # SmlI: # [CTYRAG] # 297 1401 1500 # # SpeI: # [ACTAGT] # 30 30 620 2518 # # SspI: # [AATATT] # 489 546 1029 1134 # # StyI: # [CCWWGG] # 275 901 2022 # # TaiI: # [ACGT] # 29 36 145 258 702 2028 # # TaqI: # [TCGA] # 20 32 77 82 150 193 # 351 376 501 702 714 # # TatI: # [WGTACW] # 24 261 315 380 516 799 # 903 # # TauI: # [GCSGC] # 1494 1704 # # TfiI: # [GAWTC] # 21 31 33 47 69 126 # 187 190 195 258 261 261 # 299 348 417 455 # # TseI: # [GCWGC] # 98 264 418 1170 1248 # # TsoI: # [TARCCA] # 304 396 2498 # # Tsp45I: # [GTSAC] # 1443 1755 # # Tsp4CI*: # [ACNGT] # 33 33 91 109 125 154 # 168 197 260 292 1736 # # TspDTI: # [ATGAA] # 6 6 18 22 26 42 # 66 77 107 126 164 261 # 281 441 490 525 540 # # TspEI: # [AATT] # 8 8 11 12 20 20 # 20 20 20 22 24 30 # 30 33 33 36 46 49 # 49 50 51 52 54 64 # 73 73 91 91 91 103 # 120 129 138 152 164 167 # 209 255 267 313 # # TspGWI: # [ACGGA] # 23 115 130 209 259 363 # 2099 # # TspRI: # [CASTGNN] # 30 99 115 382 450 488 # 537 1097 # # TstI: # [CACNNNNNNTCC] # 32 1581 1585 # # VspI: # [ATTAAT] # 398 804 1996 # # XbaI: # [TCTAGA] # 923 946 1329 # # XcmI: # [CCANNNNNNNNNTGG] # 30 489 645 2034 # # XhoII: # [RGATCY] # 1180 2018 # # XmnI: # [GAANNNNTTC] # 1039 2159 # # ZraI: # [GACGTC] # 27 183 2988 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3198 # Reported_sequences: 1 # Reported_hitcount: 770 #---------------------------------------