Restriction Fragments for RRP43/YCR035C



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The currently selected gene/sequence is :

RRP43/YCR035C on chromosome III from coordinates 193018 to 191834.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.32152.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 11 14 + Csp6I GTAC 11 13 . . 11 14 + RsaI GTAC 12 12 . . 16 19 + TaiI ACGT 19 15 . . 16 19 + SetI ASST 19 15 . . 16 19 + MaeII ACGT 16 18 . . 37 42 + TaqII CACCCA 53 51 . . 42 45 + TspEI AATT 41 45 . . 54 57 + SetI ASST 57 53 . . 56 61 + Eco57MI CTGRAG 77 75 . . 56 61 + Eco57I CTGAAG 77 75 . . 69 79 + BplI GAGNNNNNCTC 60 55 92 87 78 83 + Hpy178III* TCNNGA 79 81 . . 69 79 - BplI GAGNNNNNCTC 92 87 60 55 77 82 - Eco57MI CTGRAG 62 60 . . 77 82 - GsuI CTGGAG 62 60 . . 83 86 + TspEI AATT 82 86 . . 108 113 + StyI CCWWGG 108 112 . . 108 113 + SecI* CCNNGG 108 112 . . 108 113 + AvrII CCTAGG 108 112 . . 109 112 + MaeI CTAG 109 111 . . 113 117 + TfiI GAWTC 113 116 . . 113 117 + HinfI GANTC 113 116 . . 119 122 + CviJI RGCY 120 120 . . 119 122 + HaeIII GGCC 120 120 . . 122 125 + FatI CATG 121 125 . . 122 125 + NlaIII CATG 125 121 . . 122 125 + CviAII CATG 122 124 . . 127 131 + DdeI CTNAG 127 130 . . 137 141 + TspDTI ATGAA 152 150 . . 139 143 + MboII GAAGA 151 150 . . 142 147 + ApoI RAATTY 142 146 . . 143 146 + TspEI AATT 142 146 . . 158 163 + SfeI* CTRYAG 158 162 . . 179 182 + FnuDII* CGCG 180 180 . . 189 193 + FokI GGATG 202 206 . . 189 193 + BseGI GGATG 195 193 . . 187 190 - AciI CCGC 187 189 . . 190 194 - SfaNI GCATC 180 184 . . 226 229 + CviJI RGCY 227 227 . . 289 293 + MboII GAAGA 301 300 . . 296 299 + MaeI CTAG 296 298 . . 298 303 + HaeII RGCGCY 302 298 . . 299 302 + Hin6I GCGC 299 301 . . 299 302 + GlaI GCGC 300 300 . . 299 302 + HhaI GCGC 301 299 . . 301 305 + TauI GCSGC 304 301 . . 301 305 + BlsI GCNGC 303 302 . . 301 305 + BisI GCNGC 302 303 . . 302 305 + AciI CCGC 302 304 . . 303 306 + FnuDII* CGCG 304 304 . . 308 311 + MseI TTAA 308 310 . . 318 322 + FokI GGATG 331 335 . . 318 322 + BseGI GGATG 324 322 . . 328 332 + HphI GGTGA 340 339 . . 331 334 - MnlI CCTC 324 323 . . 334 338 + MboII GAAGA 346 345 . . 338 341 + TspEI AATT 337 341 . . 343 348 + AsuII TTCGAA 344 346 . . 344 347 + TaqI TCGA 344 346 . . 333 345 - AloI GAACNNNNNNTCC 357 352 325 320 349 353 + Tsp45I GTSAC 348 353 . . 349 353 + MaeIII GTNAC 348 353 . . 358 363 + BseRI GAGGAG 373 371 . . 358 361 - MnlI CCTC 351 350 . . 361 364 - MnlI CCTC 354 353 . . 369 372 + TspEI AATT 368 372 . . 372 375 + CviRI* TGCA 373 373 . . 373 383 + MwoI GCNNNNNNNGC 379 376 . . 373 383 + BstAPI GCANNNNNTGC 379 376 . . 376 379 + TspEI AATT 375 379 . . 393 398 + BetI* WCCGGW 393 397 . . 394 397 + HpaII CCGG 394 396 . . 414 417 + SetI ASST 417 413 . . 413 416 - MnlI CCTC 406 405 . . 424 429 + HaeII RGCGCY 428 424 . . 425 428 + Hin6I GCGC 425 427 . . 425 428 + GlaI GCGC 426 426 . . 425 428 + HhaI GCGC 427 425 . . 427 432 + Cac8I GCNNGC 429 429 . . 430 433 + CviRI* TGCA 431 431 . . 437 441 + TspDTI ATGAA 452 450 . . 439 443 + MboII GAAGA 451 450 . . 469 473 + TfiI GAWTC 469 472 . . 469 473 + HinfI GANTC 469 472 . . 479 482 + CviRI* TGCA 480 480 . . 485 488 + MaeI CTAG 485 487 . . 504 507 + CviJI RGCY 505 505 . . 512 515 + SetI ASST 515 511 . . 510 514 - Hin4II* CCTTC 504 503 . . 519 522 + SetI ASST 522 518 . . 519 522 + CviJI RGCY 520 520 . . 519 522 + AluI AGCT 520 520 . . 539 543 + TspDTI ATGAA 554 552 . . 541 545 + MboII GAAGA 553 552 . . 541 546 + BbvII* GAAGAC 548 552 . . 545 549 + Tsp4CI* ACNGT 547 546 . . 548 551 + Csp6I GTAC 548 550 . . 548 551 + RsaI GTAC 549 549 . . 550 553 + TaiI ACGT 553 549 . . 550 553 + SetI ASST 553 549 . . 550 553 + MaeII ACGT 550 552 . . 556 560 - TspGWI ACGGA 546 544 . . 564 569 + BciVI GTATCC 575 574 . . 567 572 + Hpy178III* TCNNGA 568 570 . . 572 576 + TspDTI ATGAA 587 585 . . 575 578 + TspEI AATT 574 578 . . 580 584 + MboII GAAGA 592 591 . . 596 600 + TspDTI ATGAA 611 609 . . 605 608 + SetI ASST 608 604 . . 613 617 + TspDTI ATGAA 628 626 . . 632 635 + TaiI ACGT 635 631 . . 632 635 + SetI ASST 635 631 . . 632 635 + MaeII ACGT 632 634 . . 648 651 + TspEI AATT 647 651 . . 656 661 + Hpy178III* TCNNGA 657 659 . . 657 662 + SmlI CTYRAG 657 661 . . 657 662 + Bce83I* CTTGAG 678 676 . . 660 664 + PleI GAGTC 668 669 . . 660 664 + MlyI GAGTC 669 669 . . 660 664 + HinfI GANTC 660 663 . . 666 672 + TspRI CASTGNN 672 663 . . 667 671 + BsrI ACTGG 672 670 . . 670 674 + BmgT120I GGNCC 671 672 . . 670 674 + AvaII GGWCC 670 673 . . 670 674 + AsuI* GGNCC 670 673 . . 673 677 - BsrI ACTGG 673 671 . . 680 683 + TaqI TCGA 680 682 . . 677 681 - MboII GAAGA 669 668 . . 676 681 - BbvII* GAAGAC 669 673 . . 693 698 + EcoRI GAATTC 693 697 . . 693 698 + ApoI RAATTY 693 697 . . 694 697 + TspEI AATT 693 697 . . 689 694 - MmeI TCCRAC 670 668 . . 705 708 + Csp6I GTAC 705 707 . . 705 708 + RsaI GTAC 706 706 . . 726 730 + MaeIII GTNAC 725 730 . . 729 732 + SetI ASST 732 728 . . 731 734 + MaeI CTAG 731 733 . . 750 755 + Cac8I GCNNGC 752 752 . . 757 761 + Hpy188I TCNGA 759 758 . . 748 758 - Hin4I GAYNNNNNVTC 771 766 739 734 754 758 - HgaI GACGC 743 748 . . 763 766 + MseI TTAA 763 765 . . 785 788 - MnlI CCTC 778 777 . . 788 792 + MboII GAAGA 800 799 . . 811 814 + TaiI ACGT 814 810 . . 811 814 + SetI ASST 814 810 . . 811 814 + MaeII ACGT 811 813 . . 815 819 + TspDTI ATGAA 830 828 . . 819 822 + TspEI AATT 818 822 . . 836 840 + DdeI CTNAG 836 839 . . 844 847 + Csp6I GTAC 844 846 . . 844 847 + RsaI GTAC 845 845 . . 846 850 + Tsp4CI* ACNGT 848 847 . . 850 853 + MseI TTAA 850 852 . . 868 873 + SspI AATATT 870 870 . . 873 876 + CviRI* TGCA 874 874 . . 886 889 + TspEI AATT 885 889 . . 913 918 + BsmI GAATGC 919 917 . . 920 923 + TspEI AATT 919 923 . . 931 935 + Hpy188I TCNGA 933 932 . . 940 944 + Hpy188I TCNGA 942 941 . . 941 946 + Eco57MI CTGRAG 962 960 . . 941 946 + Eco57I CTGAAG 962 960 . . 943 947 + MboII GAAGA 955 954 . . 946 950 + MboII GAAGA 958 957 . . 946 951 - Ksp632I* CTCTTC 941 944 . . 949 952 - MnlI CCTC 942 941 . . 955 960 + Hpy166II GTNNAC 957 957 . . 960 969 + MslI CAYNNNNRTG 964 964 . . 974 977 + TspEI AATT 973 977 . . 981 987 + TspRI CASTGNN 987 978 . . 982 986 + Tsp4CI* ACNGT 984 983 . . 998 1001 + SetI ASST 1001 997 . . 1000 1003 + MaeI CTAG 1000 1002 . . 1007 1012 + Eco57MI CTGRAG 1028 1026 . . 1007 1012 + Eco57I CTGAAG 1028 1026 . . 1015 1019 + MboII GAAGA 1027 1026 . . 1012 1015 - AciI CCGC 1012 1014 . . 1025 1030 + Hpy166II GTNNAC 1027 1027 . . 1025 1030 + BssNAI GTATAC 1027 1027 . . 1025 1030 + AccI GTMKAC 1026 1028 . . 1031 1035 + Tsp4CI* ACNGT 1033 1032 . . 1034 1037 + Csp6I GTAC 1034 1036 . . 1034 1037 + RsaI GTAC 1035 1035 . . 1038 1041 + DpnI GATC 1039 1039 . . 1038 1041 + BstKTI GATC 1040 1038 . . 1038 1047 + BsaBI GATNNNNATC 1042 1042 . . 1038 1041 + MboI GATC 1037 1041 . . 1043 1047 + BccI CCATC 1051 1052 . . 1047 1050 + MnlI CCTC 1057 1056 . . 1044 1048 - FokI GGATG 1030 1034 . . 1044 1048 - BseGI GGATG 1043 1041 . . 1051 1055 + BlsI GCNGC 1053 1052 . . 1051 1055 + BisI GCNGC 1052 1053 . . 1051 1055 + TseI GCWGC 1051 1054 . . 1051 1055 - BbvI GCAGC 1038 1042 . . 1057 1061 + Hin4II* CCTTC 1067 1066 . . 1062 1065 + SetI ASST 1065 1061 . . 1058 1063 - Eco57MI CTGRAG 1043 1041 . . 1058 1063 - Eco57I CTGAAG 1043 1041 . . 1064 1068 + MaeIII GTNAC 1063 1068 . . 1076 1079 + TspEI AATT 1075 1079 . . 1078 1081 + MseI TTAA 1078 1080 . . 1091 1096 + BseRI GAGGAG 1106 1104 . . 1091 1094 - MnlI CCTC 1084 1083 . . 1096 1101 + HaeII RGCGCY 1100 1096 . . 1094 1097 - MnlI CCTC 1087 1086 . . 1097 1100 + Hin6I GCGC 1097 1099 . . 1097 1100 + GlaI GCGC 1098 1098 . . 1097 1100 + HhaI GCGC 1099 1097 . . 1127 1130 + DpnI GATC 1128 1128 . . 1127 1130 + BstKTI GATC 1129 1127 . . 1127 1130 + MboI GATC 1126 1130 . . 1142 1145 + MaeI CTAG 1142 1144 . . 1156 1166 + Hin4I GAYNNNNNVTC 1147 1142 1179 1174 1156 1166 + Eam1105I GACNNNNNGTC 1161 1160 . . 1153 1156 - AciI CCGC 1153 1155 . . 1151 1156 - BsrBI CCGCTC 1153 1153 . . 1164 1169 + Hpy166II GTNNAC 1166 1166 . . 1164 1169 + HindII GTYRAC 1166 1166 . . 1156 1166 - Hin4I GAYNNNNNVTC 1179 1174 1147 1142 1175 1178 + MseI TTAA 1175 1177 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 159 1026 # # AciI: # [CCGC] # 32 115 141 187 710 # # AloI: # [GAACNNNNNNTCC] # 32 325 828 # # AluI: # [AGCT] # 520 665 # # ApoI: # [RAATTY] # 142 492 551 # # AsuI*: # [GGNCC] # 515 670 # # AsuII: # [TTCGAA] # 344 841 # # AvaII: # [GGWCC] # 515 670 # # AvrII: # [CCTAGG] # 108 1077 # # BbvI: # [GCAGC] # 147 1038 # # BbvII*: # [GAAGAC] # 121 516 548 # # BccI: # [CCATC] # 134 1051 # # Bce83I*: # [CTTGAG] # 507 678 # # BciVI: # [GTATCC] # 575 610 # # BetI*: # [WCCGGW] # 393 792 # # BisI: # [GCNGC] # 133 302 750 # # BlsI: # [GCNGC] # 132 303 750 # # BmgT120I: # [GGNCC] # 514 671 # # BplI: # [GAGNNNNNCTC] # 0 0 32 60 1093 # # BsaBI: # [GATNNNNATC] # 143 1042 # # BseGI: # [GGATG] # 129 142 195 719 # # BseRI: # [GAGGAG] # 79 373 733 # # BsmI: # [GAATGC] # 266 919 # # BsrBI: # [CCGCTC] # 32 1153 # # BsrI: # [ACTGG] # 1 512 672 # # BssNAI: # [GTATAC] # 158 1027 # # BstAPI: # [GCANNNNNTGC] # 379 806 # # BstKTI: # [GATC] # 56 89 1040 # # Cac8I: # [GCNNGC] # 323 429 433 # # Csp6I: # [GTAC] # 11 139 151 157 190 537 # # CviAII: # [CATG] # 122 1063 # # CviJI: # [RGCY] # 4 15 107 116 278 665 # # CviRI*: # [TGCA] # 49 58 311 373 394 # # DdeI: # [CTNAG] # 127 349 709 # # DpnI: # [GATC] # 57 89 1039 # # Eam1105I: # [GACNNNNNGTC] # 24 1161 # # Eco57I: # [CTGAAG] # 15 66 77 142 885 # # Eco57MI: # [CTGRAG] # 15 15 62 66 142 885 # # EcoRI: # [GAATTC] # 492 693 # # FatI: # [CATG] # 121 1064 # # FnuDII*: # [CGCG] # 124 180 881 # # FokI: # [GGATG] # 129 155 202 699 # # GlaI: # [GCGC] # 87 126 300 672 # # GsuI: # [CTGGAG] # 62 1123 # # HaeII: # [RGCGCY] # 85 126 302 672 # # HaeIII: # [GGCC] # 120 1065 # # HgaI: # [GACGC] # 442 743 # # HhaI: # [GCGC] # 86 126 301 672 # # Hin4I: # [GAYNNNNNVTC] # 0 0 6 32 32 376 # 739 # # Hin4II*: # [CCTTC] # 118 504 563 # # Hin6I: # [GCGC] # 88 126 299 672 # # HindII: # [GTYRAC] # 19 1166 # # HinfI: # [GANTC] # 113 191 356 525 # # HpaII: # [CCGG] # 394 791 # # HphI: # [GGTGA] # 340 845 # # Hpy166II: # [GTNNAC] # 19 70 139 957 # # Hpy178III*: # [TCNNGA] # 79 89 489 528 # # Hpy188I: # [TCNGA] # 9 174 243 759 # # Ksp632I*: # [CTCTTC] # 244 941 # # MaeI: # [CTAG] # 43 109 142 187 189 246 # 269 # # MaeII: # [ACGT] # 16 82 179 374 534 # # MaeIII: # [GTNAC] # 122 338 348 377 # # MboI: # [GATC] # 59 89 1037 # # MboII: # [GAAGA] # 3 39 45 69 77 102 # 105 131 150 151 155 158 # # MlyI: # [GAGTC] # 516 669 # # MmeI: # [TCCRAC] # 515 670 # # MnlI: # [CCTC] # 3 3 27 27 52 98 # 115 164 324 372 # # MseI: # [TTAA] # 10 87 97 228 308 455 # # MslI: # [CAYNNNNRTG] # 221 964 # # MwoI: # [GCNNNNNNNGC] # 379 806 # # NlaIII: # [CATG] # 125 1060 # # PleI: # [GAGTC] # 517 668 # # RsaI: # [GTAC] # 12 139 150 157 190 537 # # SecI*: # [CCNNGG] # 108 1077 # # SetI: # [ASST] # 7 19 27 31 38 55 # 64 82 97 98 120 187 # 360 # # SfaNI: # [GCATC] # 180 1005 # # SfeI*: # [CTRYAG] # 158 1027 # # SmlI: # [CTYRAG] # 528 657 # # SspI: # [AATATT] # 315 870 # # StyI: # [CCWWGG] # 108 1077 # # TaiI: # [ACGT] # 19 82 179 371 534 # # TaqI: # [TCGA] # 336 344 505 # # TaqII: # [CACCCA] # 53 1132 # # TauI: # [GCSGC] # 304 881 # # TfiI: # [GAWTC] # 113 356 716 # # TseI: # [GCWGC] # 134 1051 # # Tsp45I: # [GTSAC] # 348 837 # # Tsp4CI*: # [ACNGT] # 49 136 152 301 547 # # TspDTI: # [ATGAA] # 17 24 33 102 152 202 # 300 355 # # TspEI: # [AATT] # 7 31 34 41 41 46 # 54 60 67 73 102 110 # 125 195 199 # # TspGWI: # [ACGGA] # 546 639 # # TspRI: # [CASTGNN] # 198 315 672 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1185 # Reported_sequences: 1 # Reported_hitcount: 224 #---------------------------------------