Restriction Fragments for YCP4/YCR004C



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The currently selected gene/sequence is :

YCP4/YCR004C on chromosome III from coordinates 120318 to 119575.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.14675.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 18 21 + TspEI AATT 17 21 . . 31 34 + SetI ASST 34 30 . . 37 42 + SduI GDGCHC 41 37 . . 37 42 + BseSI GKGCMC 41 37 . . 47 50 + TaiI ACGT 50 46 . . 47 50 + SetI ASST 50 46 . . 47 50 + MaeII ACGT 47 49 . . 54 57 + CviJI RGCY 55 55 . . 60 63 + SetI ASST 63 59 . . 60 63 + CviJI RGCY 61 61 . . 60 63 + AluI AGCT 61 61 . . 65 68 + MseI TTAA 65 67 . . 72 75 + SetI ASST 75 71 . . 79 82 - MnlI CCTC 72 71 . . 82 86 + BlsI GCNGC 84 83 . . 82 86 + BisI GCNGC 83 84 . . 82 86 + BbvI GCAGC 94 98 . . 82 86 + TseI GCWGC 82 85 . . 83 88 + PvuII CAGCTG 85 85 . . 83 88 + NspBII* CMGCKG 85 85 . . 84 87 + SetI ASST 87 83 . . 84 87 + CviJI RGCY 85 85 . . 84 87 + AluI AGCT 85 85 . . 96 99 + SetI ASST 99 95 . . 96 99 + CviJI RGCY 97 97 . . 96 99 + AluI AGCT 97 97 . . 113 116 + TaqI TCGA 113 115 . . 115 118 - MnlI CCTC 108 107 . . 126 129 + SetI ASST 129 125 . . 131 135 + TspDTI ATGAA 146 144 . . 138 141 + MnlI CCTC 148 147 . . 141 150 + MslI CAYNNNNRTG 145 145 . . 140 144 - HphI GGTGA 132 131 . . 147 157 + Hin4I GAYNNNNNVTC 138 133 170 165 148 152 + TspDTI ATGAA 163 161 . . 157 160 + MnlI CCTC 167 166 . . 158 162 + DdeI CTNAG 158 161 . . 158 162 + BspCNI CTCAG 171 169 . . 158 162 + BseMII CTCAG 172 170 . . 159 163 + Hpy188I TCNGA 161 160 . . 155 160 - BseRI GAGGAG 146 144 . . 165 168 + SetI ASST 168 164 . . 167 172 + Eco57MI CTGRAG 188 186 . . 167 172 + Eco57I CTGAAG 188 186 . . 169 173 + MboII GAAGA 181 180 . . 169 178 + XmnI GAANNNNTTC 173 173 . . 186 192 + TspRI CASTGNN 192 183 . . 188 192 + DdeI CTNAG 188 191 . . 188 192 - BspCNI CTCAG 180 178 . . 188 192 - BseMII CTCAG 179 177 . . 195 200 + AclI AACGTT 196 198 . . 196 199 + TaiI ACGT 199 195 . . 196 199 + SetI ASST 199 195 . . 196 199 + MaeII ACGT 196 198 . . 203 206 + TaqI TCGA 203 205 . . 211 215 + HgaI GACGC 220 225 . . 211 216 + AcyI GRCGYC 212 214 . . 231 236 + MmeI TCCRAC 256 254 . . 236 239 + MaeI CTAG 236 238 . . 238 241 + SetI ASST 241 237 . . 247 250 + TspEI AATT 246 250 . . 252 257 + NaeI GCCGGC 254 254 . . 252 257 + MroNI GCCGGC 252 256 . . 252 257 + Cac8I GCNNGC 254 254 . . 252 257 + Cfr10I RCCGGY 252 256 . . 253 256 + HpaII CCGG 253 255 . . 255 258 + CviJI RGCY 256 256 . . 263 267 + BmgT120I GGNCC 264 265 . . 263 267 + AvaII GGWCC 263 266 . . 263 267 + AsuI* GGNCC 263 266 . . 266 276 + BsiYI* CCNNNNNNNGG 272 269 . . 266 269 + AciI CCGC 266 268 . . 265 270 - EciI GGCGGA 255 253 . . 283 288 + Cfr10I RCCGGY 283 287 . . 283 288 + BetI* WCCGGW 283 287 . . 283 288 + AgeI ACCGGT 283 287 . . 284 287 + HpaII CCGG 284 286 . . 296 300 + BmgT120I GGNCC 297 298 . . 296 300 + AsuI* GGNCC 296 299 . . 297 300 + CviJI RGCY 298 298 . . 297 300 + HaeIII GGCC 298 298 . . 299 304 + StyI CCWWGG 299 303 . . 299 304 + SecI* CCNNGG 299 303 . . 303 308 + SduI GDGCHC 307 303 . . 303 308 + HgiJII* GRGCYC 307 303 . . 304 307 + CviJI RGCY 305 305 . . 314 318 + BceAI ACGGC 330 332 . . 321 324 + SetI ASST 324 320 . . 321 324 + CviJI RGCY 322 322 . . 321 324 + AluI AGCT 322 322 . . 322 326 + BlsI GCNGC 324 323 . . 322 326 + BisI GCNGC 323 324 . . 322 326 + TseI GCWGC 322 325 . . 322 326 - BbvI GCAGC 309 313 . . 325 328 - AciI CCGC 325 327 . . 325 329 - FauI CCCGC 318 320 . . 340 345 + TatI WGTACW 340 344 . . 340 345 + ScaI AGTACT 342 342 . . 341 344 + Csp6I GTAC 341 343 . . 341 344 + RsaI GTAC 342 342 . . 350 354 + MaeIII GTNAC 349 354 . . 347 351 - BsrI ACTGG 347 345 . . 353 357 + TspGWI ACGGA 368 366 . . 357 360 + SetI ASST 360 356 . . 356 359 - MnlI CCTC 349 348 . . 363 368 + Hpy178III* TCNNGA 364 366 . . 371 374 + Csp6I GTAC 371 373 . . 371 374 + RsaI GTAC 372 372 . . 373 377 + Tsp4CI* ACNGT 375 374 . . 377 380 + MseI TTAA 377 379 . . 381 384 + CviJI RGCY 382 382 . . 399 402 + SetI ASST 402 398 . . 399 402 + CviJI RGCY 400 400 . . 399 402 + AluI AGCT 400 400 . . 407 411 + TspGWI ACGGA 422 420 . . 411 414 + TspEI AATT 410 414 . . 425 431 + TspRI CASTGNN 431 422 . . 426 430 + BsrI ACTGG 431 429 . . 426 431 + BfiI ACTGGG 436 435 . . 432 437 + PsiI TTATAA 434 434 . . 438 443 + EcoRI GAATTC 438 442 . . 438 443 + ApoI RAATTY 438 442 . . 439 442 + TspEI AATT 438 442 . . 441 445 - TspDTI ATGAA 431 429 . . 448 458 + MwoI GCNNNNNNNGC 454 451 . . 449 453 + DdeI CTNAG 449 452 . . 449 453 - BspCNI CTCAG 441 439 . . 449 453 - BseMII CTCAG 440 438 . . 455 460 + TsoI TARCCA 471 469 . . 456 459 + CviJI RGCY 457 457 . . 458 462 - BsrI ACTGG 458 456 . . 466 470 + MboII GAAGA 478 477 . . 470 473 + SetI ASST 473 469 . . 466 471 - Ksp632I* CTCTTC 461 464 . . 469 472 - MnlI CCTC 462 461 . . 472 477 + Hpy166II GTNNAC 474 474 . . 472 475 + Csp6I GTAC 472 474 . . 472 475 + RsaI GTAC 473 473 . . 476 480 + Tsp4CI* ACNGT 478 477 . . 481 484 + CviJI RGCY 482 482 . . 487 492 + DsaI* CCRYGG 487 491 . . 487 492 + StyI CCWWGG 487 491 . . 487 492 + NcoI CCATGG 487 491 . . 487 492 + SecI* CCNNGG 487 491 . . 488 491 + FatI CATG 487 491 . . 488 491 + NlaIII CATG 491 487 . . 488 491 + CviAII CATG 488 490 . . 499 504 + KpnI GGTACC 503 499 . . 499 504 + NlaIV GGNNCC 501 501 . . 499 504 + HgiCI* GGYRCC 499 503 . . 499 504 + Acc65I GGTACC 499 503 . . 500 503 + Csp6I GTAC 500 502 . . 500 503 + RsaI GTAC 501 501 . . 507 510 + CviRI* TGCA 508 508 . . 509 517 + AlwNI CAGNNNCTG 514 511 . . 510 516 + PpuMI RGGWCCY 511 514 . . 510 516 + DraII RGGNCCY 511 514 . . 511 515 + BmgT120I GGNCC 512 513 . . 511 515 + AvaII GGWCC 511 514 . . 511 515 + AsuI* GGNCC 511 514 . . 513 516 + SetI ASST 516 512 . . 518 522 + BceAI ACGGC 534 536 . . 520 523 + CviJI RGCY 521 521 . . 522 527 + SmlI CTYRAG 522 526 . . 523 528 + Hpy178III* TCNNGA 524 526 . . 527 538 + PpiI GAACNNNNNCTC 519 514 551 546 522 527 - Bce83I* CTTGAG 507 505 . . 527 539 + AloI GAACNNNNNNTCC 519 514 551 546 529 539 + BsaXI ACNNNNNCTCC 519 516 549 546 533 538 + Esp3I CGTCTC 539 543 . . 534 538 + BsmAI GTCTC 539 543 . . 532 536 - HgaI GACGC 521 526 . . 545 548 + TspEI AATT 544 548 . . 538 549 - CspCI CAANNNNNGTGG 562 560 527 525 552 555 + TspEI AATT 551 555 . . 560 565 + ApoI RAATTY 560 564 . . 561 564 + TspEI AATT 560 564 . . 567 570 + SetI ASST 570 566 . . 587 590 + AciI CCGC 587 589 . . 590 603 - AjuI GAANNNNNNNTTGG 615 610 583 578 606 609 + CviRI* TGCA 607 607 . . 607 617 + MwoI GCNNNNNNNGC 613 610 . . 615 618 + CviJI RGCY 616 616 . . 620 623 + CviJI RGCY 621 621 . . 623 626 + MnlI CCTC 633 632 . . 627 633 + TspRI CASTGNN 633 624 . . 636 640 + MboII GAAGA 648 647 . . 636 641 + BbvII* GAAGAC 643 647 . . 649 653 + Hpy188I TCNGA 651 650 . . 655 659 + TauI GCSGC 658 655 . . 655 659 + BlsI GCNGC 657 656 . . 655 659 + BisI GCNGC 656 657 . . 652 656 - SfaNI GCATC 642 646 . . 657 660 + CviJI RGCY 658 658 . . 655 658 - AciI CCGC 655 657 . . 659 663 + DdeI CTNAG 659 662 . . 663 667 - BsmAI GTCTC 657 661 . . 675 678 + SetI ASST 678 674 . . 675 680 + BspMI ACCTGC 684 688 . . 677 682 + PstI CTGCAG 681 677 . . 677 682 + SfeI* CTRYAG 677 681 . . 678 681 + CviRI* TGCA 679 679 . . 679 683 + BlsI GCNGC 681 680 . . 679 683 + BisI GCNGC 680 681 . . 679 683 + BbvI GCAGC 691 695 . . 679 683 + TseI GCWGC 679 682 . . 680 685 + EcoP15I CAGCAG 710 712 . . 682 686 + BlsI GCNGC 684 683 . . 682 686 + BisI GCNGC 683 684 . . 682 686 + BbvI GCAGC 694 698 . . 682 686 + TseI GCWGC 682 685 . . 684 687 + SetI ASST 687 683 . . 684 687 + CviJI RGCY 685 685 . . 684 687 + AluI AGCT 685 685 . . 685 695 + MwoI GCNNNNNNNGC 691 688 . . 692 697 + PstI CTGCAG 696 692 . . 692 697 + SfeI* CTRYAG 692 696 . . 693 696 + CviRI* TGCA 694 694 . . 702 706 - Hin4II* CCTTC 696 695 . . 706 709 - MnlI CCTC 699 698 . . 716 725 + BsaBI GATNNNNATC 720 720 . . 730 735 + TatI WGTACW 730 734 . . 726 731 - EcoP15I CAGCAG 698 700 . . 731 734 + Csp6I GTAC 731 733 . . 731 734 + RsaI GTAC 732 732 . . 733 737 + Tsp4CI* ACNGT 735 734 . . 738 741 + FatI CATG 737 741 . . 738 741 + NlaIII CATG 741 737 . . 738 741 + CviAII CATG 738 740 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 245 499 # # AciI: # [CCGC] # 59 68 89 262 266 # # AclI: # [AACGTT] # 196 548 # # AcyI: # [GRCGYC] # 212 532 # # AgeI: # [ACCGGT] # 283 461 # # AjuI: # [GAANNNNNNNTTGG] # 32 129 583 # # AloI: # [GAACNNNNNNTCC] # 32 193 519 # # AluI: # [AGCT] # 12 24 59 61 78 225 # 285 # # AlwNI: # [CAGNNNCTG] # 230 514 # # ApoI: # [RAATTY] # 122 184 438 # # AsuI*: # [GGNCC] # 33 215 233 263 # # AvaII: # [GGWCC] # 233 248 263 # # BbvI: # [GCAGC] # 3 50 94 215 382 # # BbvII*: # [GAAGAC] # 101 643 # # Bce83I*: # [CTTGAG] # 237 507 # # BceAI: # [ACGGC] # 204 210 330 # # BetI*: # [WCCGGW] # 283 461 # # BfiI: # [ACTGGG] # 308 436 # # BisI: # [GCNGC] # 3 24 61 83 240 333 # # BlsI: # [GCNGC] # 3 24 60 84 240 333 # # BmgT120I: # [GGNCC] # 33 215 232 264 # # BsaBI: # [GATNNNNATC] # 24 720 # # BsaXI: # [ACNNNNNCTCC] # 30 195 519 # # BseMII: # [CTCAG] # 7 172 261 304 # # BseRI: # [GAGGAG] # 146 598 # # BseSI: # [GKGCMC] # 41 703 # # BsiYI*: # [CCNNNNNNNGG] # 272 472 # # BsmAI: # [GTCTC] # 87 118 539 # # BspCNI: # [CTCAG] # 9 171 261 303 # # BspMI: # [ACCTGC] # 60 684 # # BsrI: # [ACTGG] # 27 84 286 347 # # Cac8I: # [GCNNGC] # 254 490 # # Cfr10I: # [RCCGGY] # 31 252 461 # # Csp6I: # [GTAC] # 13 28 30 101 231 341 # # CspCI: # [CAANNNNNGTGG] # 35 182 527 # # CviAII: # [CATG] # 6 250 488 # # CviJI: # [RGCY] # 5 6 7 12 17 18 # 24 25 27 37 39 42 # 55 57 59 60 95 159 # # CviRI*: # [TGCA] # 15 50 72 99 508 # # DdeI: # [CTNAG] # 30 85 158 210 261 # # DraII: # [RGGNCCY] # 233 511 # # DsaI*: # [CCRYGG] # 257 487 # # EciI: # [GGCGGA] # 255 489 # # Eco57I: # [CTGAAG] # 188 556 # # Eco57MI: # [CTGRAG] # 188 556 # # EcoP15I: # [CAGCAG] # 12 34 698 # # EcoRI: # [GAATTC] # 306 438 # # Esp3I: # [CGTCTC] # 205 539 # # FatI: # [CATG] # 7 250 487 # # FauI: # [CCCGC] # 318 426 # # HaeIII: # [GGCC] # 298 446 # # HgaI: # [GACGC] # 220 223 301 # # HgiCI*: # [GGYRCC] # 245 499 # # HgiJII*: # [GRGCYC] # 307 437 # # Hin4I: # [GAYNNNNNVTC] # 32 138 574 # # Hin4II*: # [CCTTC] # 48 696 # # HpaII: # [CCGG] # 31 253 460 # # HphI: # [GGTGA] # 132 612 # # Hpy166II: # [GTNNAC] # 270 474 # # Hpy178III*: # [TCNNGA] # 160 220 364 # # Hpy188I: # [TCNGA] # 93 161 490 # # KpnI: # [GGTACC] # 241 503 # # Ksp632I*: # [CTCTTC] # 283 461 # # MaeI: # [CTAG] # 236 508 # # MaeII: # [ACGT] # 47 149 548 # # MaeIII: # [GTNAC] # 349 395 # # MboII: # [GAAGA] # 96 170 181 297 # # MmeI: # [TCCRAC] # 256 488 # # MnlI: # [CCTC] # 19 36 40 45 66 72 # 113 171 182 # # MroNI: # [GCCGGC] # 252 492 # # MseI: # [TTAA] # 65 312 367 # # MslI: # [CAYNNNNRTG] # 145 599 # # MwoI: # [GCNNNNNNNGC] # 53 78 159 454 # # NaeI: # [GCCGGC] # 254 490 # # NcoI: # [CCATGG] # 257 487 # # NlaIII: # [CATG] # 3 250 491 # # NlaIV: # [GGNNCC] # 243 501 # # NspBII*: # [CMGCKG] # 85 659 # # PpiI: # [GAACNNNNNCTC] # 32 193 519 # # PpuMI: # [RGGWCCY] # 233 511 # # PsiI: # [TTATAA] # 310 434 # # PstI: # [CTGCAG] # 15 48 681 # # PvuII: # [CAGCTG] # 85 659 # # RsaI: # [GTAC] # 12 28 30 101 231 342 # # ScaI: # [AGTACT] # 342 402 # # SduI: # [GDGCHC] # 41 266 437 # # SecI*: # [CCNNGG] # 188 257 299 # # SetI: # [ASST] # 9 12 12 12 13 16 # 30 31 34 36 39 42 # 42 43 54 57 71 83 # 108 # # SfaNI: # [GCATC] # 102 642 # # SfeI*: # [CTRYAG] # 15 52 677 # # SmlI: # [CTYRAG] # 222 522 # # StyI: # [CCWWGG] # 188 257 299 # # TaiI: # [ACGT] # 50 149 545 # # TaqI: # [TCGA] # 90 113 541 # # TatI: # [WGTACW] # 14 340 390 # # TauI: # [GCSGC] # 86 658 # # TseI: # [GCWGC] # 3 62 82 240 357 # # TsoI: # [TARCCA] # 273 471 # # Tsp4CI*: # [ACNGT] # 9 103 257 375 # # TspDTI: # [ATGAA] # 17 146 268 313 # # TspEI: # [AATT] # 7 9 17 28 106 164 # 184 229 # # TspGWI: # [ACGGA] # 54 322 368 # # TspRI: # [CASTGNN] # 111 192 202 239 # # XmnI: # [GAANNNNTTC] # 173 571 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 744 # Reported_sequences: 1 # Reported_hitcount: 229 #---------------------------------------