Restriction Fragments for YCLWomega1



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YCLWomega1 on chromosome III from coordinates 1179 to 1429.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30526.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 17 + BstEII GGTNACC 11 16 . . 12 16 + MaeIII GTNAC 11 16 . . 24 27 + FatI CATG 23 27 . . 24 27 + NlaIII CATG 27 23 . . 24 27 + CviAII CATG 24 26 . . 53 57 + Hpy188I TCNGA 55 54 . . 102 105 + SetI ASST 105 101 . . 103 106 + MnlI CCTC 113 112 . . 110 115 + BetI* WCCGGW 110 114 . . 111 114 + HpaII CCGG 111 113 . . 116 121 + TatI WGTACW 116 120 . . 116 121 + ScaI AGTACT 118 118 . . 117 120 + Csp6I GTAC 117 119 . . 117 120 + RsaI GTAC 118 118 . . 120 125 + SmlI CTYRAG 120 124 . . 120 125 + AflII CTTAAG 120 124 . . 121 124 + MseI TTAA 121 123 . . 129 134 + SfeI* CTRYAG 129 133 . . 131 135 + Tsp4CI* ACNGT 133 132 . . 142 145 + Csp6I GTAC 142 144 . . 142 145 + RsaI GTAC 143 143 . . 153 157 + MaeIII GTNAC 152 157 . . 163 166 + TspEI AATT 162 166 . . 171 180 + MslI CAYNNNNRTG 175 175 . . 177 180 + FatI CATG 176 180 . . 177 180 + NlaIII CATG 180 176 . . 177 180 + CviAII CATG 177 179 . . 191 194 + SetI ASST 194 190 . . 192 196 + BmgT120I GGNCC 193 194 . . 192 196 + AvaII GGWCC 192 195 . . 192 196 + AsuI* GGNCC 192 195 . . 189 193 - Hin4II* CCTTC 183 182 . . 217 220 + SetI ASST 220 216 . . 224 227 + SetI ASST 227 223 . . 224 227 + CviJI RGCY 225 225 . . 224 227 + AluI AGCT 225 225 . . 226 229 + MaeI CTAG 226 228 . . 229 232 + DpnI GATC 230 230 . . 229 232 + BstKTI GATC 231 229 . . 229 232 + MboI GATC 228 232 . . 235 238 + TspEI AATT 234 238 . . 237 241 - TspDTI ATGAA 227 225 . . 243 246 + TaiI ACGT 246 242 . . 243 246 + SetI ASST 246 242 . . 243 246 + MaeII ACGT 243 245 . . 245 250 + Hpy166II GTNNAC 247 247 . . 245 250 + HindII GTYRAC 247 247 . . #--------------------------------------- # # # AflII: # [CTTAAG] # 120 131 # # AluI: # [AGCT] # 26 225 # # AsuI*: # [GGNCC] # 59 192 # # AvaII: # [GGWCC] # 59 192 # # BetI*: # [WCCGGW] # 110 141 # # BmgT120I: # [GGNCC] # 58 193 # # BstEII: # [GGTNACC] # 11 240 # # BstKTI: # [GATC] # 20 231 # # Csp6I: # [GTAC] # 25 109 117 # # CviAII: # [CATG] # 24 74 153 # # CviJI: # [RGCY] # 26 225 # # DpnI: # [GATC] # 21 230 # # FatI: # [CATG] # 23 75 153 # # Hin4II*: # [CCTTC] # 68 183 # # HindII: # [GTYRAC] # 4 247 # # HpaII: # [CCGG] # 111 140 # # Hpy166II: # [GTNNAC] # 4 247 # # Hpy188I: # [TCNGA] # 55 196 # # MaeI: # [CTAG] # 25 226 # # MaeII: # [ACGT] # 8 243 # # MaeIII: # [GTNAC] # 11 99 141 # # MboI: # [GATC] # 23 228 # # MnlI: # [CCTC] # 113 138 # # MseI: # [TTAA] # 121 130 # # MslI: # [CAYNNNNRTG] # 76 175 # # NlaIII: # [CATG] # 27 71 153 # # RsaI: # [GTAC] # 25 108 118 # # ScaI: # [AGTACT] # 118 133 # # SetI: # [ASST] # 5 7 19 26 89 105 # # SfeI*: # [CTRYAG] # 122 129 # # SmlI: # [CTYRAG] # 120 131 # # TaiI: # [ACGT] # 5 246 # # TatI: # [WGTACW] # 116 135 # # Tsp4CI*: # [ACNGT] # 118 133 # # TspDTI: # [ATGAA] # 24 227 # # TspEI: # [AATT] # 17 72 162 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 251 # Reported_sequences: 1 # Reported_hitcount: 47 #---------------------------------------