Restriction Fragments for YCLWdelta5



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YCLWdelta5 on chromosome III from coordinates 90438 to 90769.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.9133.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 42 45 + TaiI ACGT 45 41 . . 42 45 + SetI ASST 45 41 . . 42 45 + MaeII ACGT 42 44 . . 44 48 + MaeIII GTNAC 43 48 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 74 78 + MboII GAAGA 86 85 . . 80 84 + HgaI GACGC 89 94 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 108 111 + TspEI AATT 107 111 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 148 152 + BinI* GGATC 156 157 . . 149 152 + DpnI GATC 150 150 . . 149 152 + BstKTI GATC 151 149 . . 149 152 + MboI GATC 148 152 . . 154 158 + TspDTI ATGAA 169 167 . . 157 162 + SspI AATATT 159 159 . . 161 164 + MseI TTAA 161 163 . . 184 189 + SspI AATATT 186 186 . . 195 198 + TspEI AATT 194 198 . . 205 208 + TspEI AATT 204 208 . . 208 211 + CviRI* TGCA 209 209 . . 212 216 + TfiI GAWTC 212 215 . . 212 216 + HinfI GANTC 212 215 . . 216 226 + BsiYI* CCNNNNNNNGG 222 219 . . 226 230 + TfiI GAWTC 226 229 . . 226 230 + HinfI GANTC 226 229 . . 237 244 + PspXI VCTCGAGB 238 242 . . 237 240 + MnlI CCTC 247 246 . . 237 244 + AbsI CCTCGAGG 238 242 . . 238 243 + SmlI CTYRAG 238 242 . . 238 243 + XhoI CTCGAG 238 242 . . 238 243 + BmeT110I CYCGRG 239 241 . . 238 243 + AvaI CYCGRG 238 242 . . 239 242 + TaqI TCGA 239 241 . . 241 246 + BseRI GAGGAG 256 254 . . 241 244 - MnlI CCTC 234 233 . . 252 255 + MaeI CTAG 252 254 . . 267 270 + SetI ASST 270 266 . . 271 276 + SspI AATATT 273 273 . . 286 289 + MseI TTAA 286 288 . . 295 299 + TfiI GAWTC 295 298 . . 295 299 + HinfI GANTC 295 298 . . 305 308 + TspEI AATT 304 308 . . 327 331 - MboII GAAGA 319 318 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 94 238 # # AluI: # [AGCT] # 119 213 # # AvaI: # [CYCGRG] # 94 238 # # BinI*: # [GGATC] # 156 176 # # BmeT110I: # [CYCGRG] # 93 239 # # BseRI: # [GAGGAG] # 76 256 # # BsiYI*: # [CCNNNNNNNGG] # 110 222 # # BstKTI: # [GATC] # 151 181 # # CviJI: # [RGCY] # 119 213 # # CviRI*: # [TGCA] # 123 209 # # DpnI: # [GATC] # 150 182 # # HgaI: # [GACGC] # 89 243 # # Hin4I: # [GAYNNNNNVTC] # 32 83 217 # # HinfI: # [GANTC] # 14 37 69 212 # # MaeI: # [CTAG] # 15 33 80 204 # # MaeII: # [ACGT] # 42 290 # # MaeIII: # [GTNAC] # 43 289 # # MboI: # [GATC] # 148 184 # # MboII: # [GAAGA] # 13 86 233 # # MnlI: # [CCTC] # 13 85 234 # # MseI: # [TTAA] # 46 125 161 # # PspXI: # [VCTCGAGB] # 94 238 # # SetI: # [ASST] # 45 62 76 149 # # SmlI: # [CTYRAG] # 94 238 # # SpeI: # [ACTAGT] # 15 32 285 # # SspI: # [AATATT] # 27 59 87 159 # # TaiI: # [ACGT] # 45 287 # # TaqI: # [TCGA] # 93 239 # # TfiI: # [GAWTC] # 14 37 69 212 # # Tsp4CI*: # [ACNGT] # 67 265 # # TspDTI: # [ATGAA] # 163 169 # # TspEI: # [AATT] # 10 28 87 100 107 # # XhoI: # [CTCGAG] # 94 238 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 332 # Reported_sequences: 1 # Reported_hitcount: 50 #---------------------------------------