Restriction Fragments for YCLWdelta15



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YCLWdelta15 on chromosome III from coordinates 82700 to 83036.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.4159.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 74 78 + MboII GAAGA 86 85 . . 80 84 + HgaI GACGC 89 94 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 108 111 + TspEI AATT 107 111 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 148 152 + BinI* GGATC 156 157 . . 149 152 + DpnI GATC 150 150 . . 149 152 + BstKTI GATC 151 149 . . 149 152 + MboI GATC 148 152 . . 154 158 + TspDTI ATGAA 169 167 . . 173 177 + TspGWI ACGGA 188 186 . . 180 183 - MnlI CCTC 173 172 . . 180 190 - Hin4I GAYNNNNNVTC 203 198 171 166 193 198 + SspI AATATT 195 195 . . 215 219 + TfiI GAWTC 215 218 . . 215 219 + HinfI GANTC 215 218 . . 219 229 + BsiYI* CCNNNNNNNGG 225 222 . . 229 233 + TfiI GAWTC 229 232 . . 226 229 - MnlI CCTC 219 218 . . 229 233 + HinfI GANTC 229 232 . . 240 247 + PspXI VCTCGAGB 241 245 . . 240 243 + MnlI CCTC 250 249 . . 240 247 + AbsI CCTCGAGG 241 245 . . 241 246 + SmlI CTYRAG 241 245 . . 241 246 + XhoI CTCGAG 241 245 . . 241 246 + BmeT110I CYCGRG 242 244 . . 241 246 + AvaI CYCGRG 241 245 . . 242 245 + TaqI TCGA 242 244 . . 244 249 + BseRI GAGGAG 259 257 . . 244 247 - MnlI CCTC 237 236 . . 255 258 + MaeI CTAG 255 257 . . 267 272 + Hpy166II GTNNAC 269 269 . . 267 272 + BssNAI GTATAC 269 269 . . 267 272 + AccI GTMKAC 268 270 . . 271 274 + SetI ASST 274 270 . . 275 280 + SspI AATATT 277 277 . . 283 286 + CviJI RGCY 284 284 . . 300 304 + TfiI GAWTC 300 303 . . 300 304 + HinfI GANTC 300 303 . . 310 313 + TspEI AATT 309 313 . . 324 328 - HphI GGTGA 316 315 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 96 241 # # AccI: # [GTMKAC] # 69 268 # # AluI: # [AGCT] # 119 218 # # AvaI: # [CYCGRG] # 96 241 # # BinI*: # [GGATC] # 156 181 # # BmeT110I: # [CYCGRG] # 95 242 # # BseRI: # [GAGGAG] # 78 259 # # BsiYI*: # [CCNNNNNNNGG] # 112 225 # # BssNAI: # [GTATAC] # 68 269 # # BstKTI: # [GATC] # 151 186 # # CviJI: # [RGCY] # 53 119 165 # # DpnI: # [GATC] # 150 187 # # HgaI: # [GACGC] # 89 248 # # Hin4I: # [GAYNNNNNVTC] # 32 32 56 83 134 # # HinfI: # [GANTC] # 14 37 71 215 # # HphI: # [GGTGA] # 21 316 # # Hpy166II: # [GTNNAC] # 68 269 # # MaeI: # [CTAG] # 15 33 82 207 # # MboI: # [GATC] # 148 189 # # MboII: # [GAAGA] # 86 251 # # MnlI: # [CCTC] # 13 18 46 87 173 # # PspXI: # [VCTCGAGB] # 96 241 # # SetI: # [ASST] # 63 121 153 # # SmlI: # [CTYRAG] # 96 241 # # SpeI: # [ACTAGT] # 15 32 290 # # SspI: # [AATATT] # 60 82 195 # # TaqI: # [TCGA] # 95 242 # # TfiI: # [GAWTC] # 14 37 71 215 # # Tsp4CI*: # [ACNGT] # 67 270 # # TspDTI: # [ATGAA] # 168 169 # # TspEI: # [AATT] # 28 107 202 # # TspGWI: # [ACGGA] # 149 188 # # XhoI: # [CTCGAG] # 96 241 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 337 # Reported_sequences: 1 # Reported_hitcount: 48 #---------------------------------------