Restriction Fragments for KRR1/YCL059C



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The currently selected gene/sequence is :

KRR1/YCL059C on chromosome III from coordinates 23379 to 22429.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.31119.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + Hpy166II GTNNAC 8 8 . . 6 11 + AccI GTMKAC 7 9 . . 27 30 + SetI ASST 30 26 . . 28 33 + StyI CCWWGG 28 32 . . 28 33 + SecI* CCNNGG 28 32 . . 37 41 + TspGWI ACGGA 52 50 . . 33 38 - BciVI GTATCC 27 26 . . 39 43 + FokI GGATG 52 56 . . 39 43 + BseGI GGATG 45 43 . . 57 61 + MboII GAAGA 69 68 . . 64 69 + BseRI GAGGAG 79 77 . . 64 67 - MnlI CCTC 57 56 . . 71 74 + MseI TTAA 71 73 . . 76 80 + MboII GAAGA 88 87 . . 76 81 - Ksp632I* CTCTTC 71 74 . . 79 82 - MnlI CCTC 72 71 . . 82 92 + Hin4I GAYNNNNNVTC 73 68 105 100 88 92 + SfaNI GCATC 97 101 . . 91 96 + BetI* WCCGGW 91 95 . . 82 92 - Hin4I GAYNNNNNVTC 105 100 73 68 92 95 + HpaII CCGG 92 94 . . 89 93 - FokI GGATG 75 79 . . 89 93 - BseGI GGATG 88 86 . . 95 100 + Hpy166II GTNNAC 97 97 . . 95 100 + HindII GTYRAC 97 97 . . 99 102 + SetI ASST 102 98 . . 107 112 + Eco57MI CTGRAG 128 126 . . 107 112 + Eco57I CTGAAG 128 126 . . 109 113 + MboII GAAGA 121 120 . . 112 116 + PleI GAGTC 120 121 . . 112 116 + MlyI GAGTC 121 121 . . 112 116 + HinfI GANTC 112 115 . . 109 114 - Ksp632I* CTCTTC 104 107 . . 116 120 - BsrI ACTGG 116 114 . . 152 156 + MaeIII GTNAC 151 156 . . 159 163 + MboII GAAGA 171 170 . . 159 164 + BbvII* GAAGAC 166 170 . . 178 182 + MaeIII GTNAC 177 182 . . 185 190 + SduI GDGCHC 189 185 . . 185 190 + HgiJII* GRGCYC 189 185 . . 186 189 + CviJI RGCY 187 187 . . 189 194 + XbaI TCTAGA 189 193 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 190 193 + MaeI CTAG 190 192 . . 216 221 + XbaI TCTAGA 216 220 . . 216 221 + Hpy178III* TCNNGA 217 219 . . 217 220 + MaeI CTAG 217 219 . . 227 230 + TaqI TCGA 227 229 . . 231 234 + SetI ASST 234 230 . . 229 233 - Hin4II* CCTTC 223 222 . . 241 245 + Tsp4CI* ACNGT 243 242 . . 254 257 + MaeI CTAG 254 256 . . 268 271 + DpnI GATC 269 269 . . 268 271 + BstKTI GATC 270 268 . . 268 271 + MboI GATC 267 271 . . 271 275 + FauI CCCGC 279 281 . . 268 272 - BinI* GGATC 262 263 . . 272 275 + AciI CCGC 272 274 . . 288 291 + CviJI RGCY 289 289 . . 299 304 + BclI TGATCA 299 303 . . 300 303 + DpnI GATC 301 301 . . 300 303 + BstKTI GATC 302 300 . . 300 303 + MboI GATC 299 303 . . 293 303 - Hin4I GAYNNNNNVTC 316 311 284 279 305 308 + TspEI AATT 304 308 . . 316 321 + XhoII RGATCY 316 320 . . 317 320 + DpnI GATC 318 318 . . 317 320 + BstKTI GATC 319 317 . . 317 320 + MboI GATC 316 320 . . 317 321 - BinI* GGATC 311 312 . . 319 323 - TspGWI ACGGA 309 307 . . 330 334 + FauI CCCGC 338 340 . . 331 334 + AciI CCGC 331 333 . . 333 338 + Cac8I GCNNGC 335 335 . . 336 339 + CviJI RGCY 337 337 . . 341 344 + MseI TTAA 341 343 . . 337 341 - BceAI ACGGC 322 324 . . 344 349 + XhoII RGATCY 344 348 . . 345 348 + DpnI GATC 346 346 . . 345 348 + BstKTI GATC 347 345 . . 345 348 + MboI GATC 344 348 . . 345 349 - BinI* GGATC 339 340 . . 360 363 + FatI CATG 359 363 . . 360 363 + NlaIII CATG 363 359 . . 360 363 + CviAII CATG 360 362 . . 364 369 + NspI RCATGY 368 364 . . 364 369 + Cac8I GCNNGC 366 366 . . 364 369 + SphI GCATGC 368 364 . . 365 368 + FatI CATG 364 368 . . 365 368 + NlaIII CATG 368 364 . . 365 368 + CviAII CATG 365 367 . . 369 373 + Hpy99I CGWCG 373 368 . . 371 374 + TaiI ACGT 374 370 . . 371 374 + SetI ASST 374 370 . . 371 374 + MaeII ACGT 371 373 . . 377 380 + MseI TTAA 377 379 . . 381 384 + TspEI AATT 380 384 . . 388 391 + TspEI AATT 387 391 . . 394 398 + MaeIII GTNAC 393 398 . . 409 412 + SetI ASST 412 408 . . 416 421 + Hpy178III* TCNNGA 417 419 . . 422 426 + MboII GAAGA 434 433 . . 422 427 + BbvII* GAAGAC 429 433 . . 425 433 + Tth111I GACNNNGTC 428 429 . . 429 432 + TaiI ACGT 432 428 . . 429 432 + SetI ASST 432 428 . . 429 432 + MaeII ACGT 429 431 . . 438 444 + DraII RGGNCCY 439 442 . . 439 442 + CviJI RGCY 440 440 . . 439 442 + HaeIII GGCC 440 440 . . 439 443 + BmgT120I GGNCC 440 441 . . 439 443 + AsuI* GGNCC 439 442 . . 446 450 + Tsp4CI* ACNGT 448 447 . . 456 461 + AhaIII* TTTAAA 458 458 . . 457 460 + MseI TTAA 457 459 . . 462 465 + CviJI RGCY 463 463 . . 485 489 + MaeIII GTNAC 484 489 . . 493 496 + MaeI CTAG 493 495 . . 495 500 + TatI WGTACW 495 499 . . 496 499 + Csp6I GTAC 496 498 . . 496 499 + RsaI GTAC 497 497 . . 501 504 + SetI ASST 504 500 . . 503 507 + MaeIII GTNAC 502 507 . . 508 512 + Tsp4CI* ACNGT 510 509 . . 518 523 + DsaI* CCRYGG 518 522 . . 518 523 + StyI CCWWGG 518 522 . . 518 523 + NcoI CCATGG 518 522 . . 518 523 + SecI* CCNNGG 518 522 . . 519 522 + FatI CATG 518 522 . . 519 522 + NlaIII CATG 522 518 . . 519 522 + CviAII CATG 519 521 . . 522 528 + SanDI GGGWCCC 523 526 . . 522 528 + PpuMI RGGWCCY 523 526 . . 522 528 + DraII RGGNCCY 523 526 . . 522 527 + NlaIV GGNNCC 524 524 . . 523 528 + NlaIV GGNNCC 525 525 . . 523 527 + BmgT120I GGNCC 524 525 . . 523 527 + AvaII GGWCC 523 526 . . 523 527 + AsuI* GGNCC 523 526 . . 526 536 + BsiYI* CCNNNNNNNGG 532 529 . . 527 531 + Hin4II* CCTTC 537 536 . . 524 528 - BslFI GGGAC 509 513 . . 535 538 + CviJI RGCY 536 536 . . 540 544 - Hin4II* CCTTC 534 533 . . 545 548 + SetI ASST 548 544 . . 546 550 + BmgT120I GGNCC 547 548 . . 546 550 + AvaII GGWCC 546 549 . . 546 550 + AsuI* GGNCC 546 549 . . 544 547 - MnlI CCTC 537 536 . . 550 554 + Hpy99I CGWCG 554 549 . . 548 552 - TspGWI ACGGA 538 536 . . 552 555 + TaqI TCGA 552 554 . . 562 566 + MboII GAAGA 574 573 . . 571 575 + TspDTI ATGAA 586 584 . . 577 582 + SspI AATATT 579 579 . . 582 586 - HphI GGTGA 574 573 . . 605 608 + TspEI AATT 604 608 . . 606 611 + VspI ATTAAT 607 609 . . 607 610 + MseI TTAA 607 609 . . 623 626 + TspEI AATT 622 626 . . 635 638 + CviJI RGCY 636 636 . . 635 638 + HaeIII GGCC 636 636 . . 644 649 + TsoI TARCCA 660 658 . . 645 648 + CviJI RGCY 646 646 . . 650 654 + TspDTI ATGAA 665 663 . . 652 656 + MboII GAAGA 664 663 . . 661 666 + Hpy178III* TCNNGA 662 664 . . 665 669 + TfiI GAWTC 665 668 . . 665 669 + HinfI GANTC 665 668 . . 675 678 + FatI CATG 674 678 . . 675 678 + NlaIII CATG 678 674 . . 675 678 + CviAII CATG 675 677 . . 680 683 + MseI TTAA 680 682 . . 684 688 + MboII GAAGA 696 695 . . 684 689 - Ksp632I* CTCTTC 679 682 . . 687 690 - MnlI CCTC 680 679 . . 695 700 + BalI TGGCCA 697 697 . . 695 700 + CfrI YGGCCR 695 699 . . 696 699 + CviJI RGCY 697 697 . . 696 699 + HaeIII GGCC 697 697 . . 714 718 + MboII GAAGA 726 725 . . 717 720 + DpnI GATC 718 718 . . 717 720 + BstKTI GATC 719 717 . . 717 720 + MboI GATC 716 720 . . 719 723 + Hpy188I TCNGA 721 720 . . 725 728 + TaiI ACGT 728 724 . . 725 728 + SetI ASST 728 724 . . 725 728 + MaeII ACGT 725 727 . . 726 730 + Hpy99I CGWCG 730 725 . . 728 731 + TaqI TCGA 728 730 . . 737 740 + SetI ASST 740 736 . . 735 739 - Hin4II* CCTTC 729 728 . . 754 757 + MnlI CCTC 764 763 . . 763 768 + MfeI CAATTG 763 767 . . 764 767 + TspEI AATT 763 767 . . 770 773 + MaeI CTAG 770 772 . . 776 779 + SetI ASST 779 775 . . 789 792 + TspEI AATT 788 792 . . 799 803 + HphI GGTGA 811 810 . . 810 815 + SmlI CTYRAG 810 814 . . 810 815 + AflII CTTAAG 810 814 . . 811 814 + MseI TTAA 811 813 . . 832 836 + TspDTI ATGAA 847 845 . . 834 838 + MboII GAAGA 846 845 . . 839 842 + TspEI AATT 838 842 . . 841 844 + MseI TTAA 841 843 . . 878 881 + TaqI TCGA 878 880 . . 889 893 + MboII GAAGA 901 900 . . 889 894 - Ksp632I* CTCTTC 884 887 . . 907 911 - TspDTI ATGAA 897 895 . . 912 915 + SetI ASST 915 911 . . 912 915 + CviJI RGCY 913 913 . . 912 915 + AluI AGCT 913 913 . . 915 920 + Hpy178III* TCNNGA 916 918 . . 915 920 + BetI* WCCGGW 915 919 . . 915 920 + BspMII* TCCGGA 915 919 . . 916 919 + HpaII CCGG 916 918 . . 919 923 + MboII GAAGA 931 930 . . 922 926 + MboII GAAGA 934 933 . . 935 938 + CviJI RGCY 936 936 . . 946 949 + TspEI AATT 945 949 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 7 944 # # AciI: # [CCGC] # 59 272 620 # # AflII: # [CTTAAG] # 141 810 # # AhaIII*: # [TTTAAA] # 458 493 # # AluI: # [AGCT] # 38 913 # # AsuI*: # [GGNCC] # 23 84 405 439 # # AvaII: # [GGWCC] # 23 405 523 # # BalI: # [TGGCCA] # 254 697 # # BbvII*: # [GAAGAC] # 166 263 522 # # BceAI: # [ACGGC] # 322 629 # # BciVI: # [GTATCC] # 27 924 # # BclI: # [TGATCA] # 299 652 # # BetI*: # [WCCGGW] # 36 91 824 # # BinI*: # [GGATC] # 28 49 262 612 # # BmgT120I: # [GGNCC] # 23 84 404 440 # # BseGI: # [GGATG] # 43 45 863 # # BseRI: # [GAGGAG] # 79 872 # # BsiYI*: # [CCNNNNNNNGG] # 419 532 # # BslFI: # [GGGAC] # 442 509 # # BspMII*: # [TCCGGA] # 36 915 # # BsrI: # [ACTGG] # 116 835 # # BstKTI: # [GATC] # 17 28 32 232 270 372 # # Cac8I: # [GCNNGC] # 31 335 585 # # CfrI: # [YGGCCR] # 256 695 # # Csp6I: # [GTAC] # 455 496 # # CviAII: # [CATG] # 5 154 156 276 360 # # CviJI: # [RGCY] # 10 15 23 23 48 51 # 73 100 102 103 187 216 # # DpnI: # [GATC] # 17 28 32 233 269 372 # # DraII: # [RGGNCCY] # 84 428 439 # # DsaI*: # [CCRYGG] # 433 518 # # Eco57I: # [CTGAAG] # 128 823 # # Eco57MI: # [CTGRAG] # 128 823 # # FatI: # [CATG] # 5 154 156 277 359 # # FauI: # [CCCGC] # 59 279 613 # # FokI: # [GGATG] # 23 52 876 # # HaeIII: # [GGCC] # 61 196 254 440 # # HgiJII*: # [GRGCYC] # 189 762 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 73 179 # 635 # # Hin4II*: # [CCTTC] # 3 192 222 223 311 # # HindII: # [GTYRAC] # 97 854 # # HinfI: # [GANTC] # 112 286 553 # # HpaII: # [CCGG] # 35 92 824 # # HphI: # [GGTGA] # 140 237 574 # # Hpy166II: # [GTNNAC] # 8 89 854 # # Hpy178III*: # [TCNNGA] # 27 35 190 200 245 254 # # Hpy188I: # [TCNGA] # 230 721 # # Hpy99I: # [CGWCG] # 176 181 221 373 # # Ksp632I*: # [CTCTTC] # 33 67 71 205 575 # # MaeI: # [CTAG] # 27 37 181 190 239 277 # # MaeII: # [ACGT] # 58 226 296 371 # # MaeIII: # [GTNAC] # 18 26 91 151 216 449 # # MboI: # [GATC] # 17 28 32 235 267 372 # # MboII: # [GAAGA] # 3 17 19 30 30 32 # 33 50 55 69 90 120 # 140 263 # # MfeI: # [CAATTG] # 188 763 # # MlyI: # [GAGTC] # 121 830 # # MnlI: # [CCTC] # 15 57 84 143 187 465 # # MseI: # [TTAA] # 30 36 71 73 80 110 # 131 150 270 # # NcoI: # [CCATGG] # 433 518 # # NlaIII: # [CATG] # 5 154 156 273 363 # # NlaIV: # [GGNNCC] # 1 426 524 # # NspI: # [RCATGY] # 368 583 # # PleI: # [GAGTC] # 120 831 # # PpuMI: # [RGGWCCY] # 428 523 # # RsaI: # [GTAC] # 454 497 # # SanDI: # [GGGWCCC] # 428 523 # # SduI: # [GDGCHC] # 189 762 # # SecI*: # [CCNNGG] # 28 433 490 # # SetI: # [ASST] # 12 20 30 36 38 39 # 44 72 72 132 136 140 # 180 # # SfaNI: # [GCATC] # 97 854 # # SmlI: # [CTYRAG] # 141 810 # # SphI: # [GCATGC] # 368 583 # # SspI: # [AATATT] # 372 579 # # StyI: # [CCWWGG] # 28 433 490 # # TaiI: # [ACGT] # 58 223 296 374 # # TaqI: # [TCGA] # 73 150 176 227 325 # # TatI: # [WGTACW] # 456 495 # # TfiI: # [GAWTC] # 286 665 # # TsoI: # [TARCCA] # 291 660 # # Tsp4CI*: # [ACNGT] # 62 205 243 441 # # TspDTI: # [ATGAA] # 50 54 79 182 586 # # TspEI: # [AATT] # 6 7 18 25 50 76 # 107 141 217 304 # # TspGWI: # [ACGGA] # 52 229 257 413 # # Tth111I: # [GACNNNGTC] # 428 523 # # VspI: # [ATTAAT] # 344 607 # # XbaI: # [TCTAGA] # 27 189 735 # # XhoII: # [RGATCY] # 28 316 607 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 951 # Reported_sequences: 1 # Reported_hitcount: 221 #---------------------------------------