Restriction Fragments for PDI1/YCL043C



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The currently selected gene/sequence is :

PDI1/YCL043C on chromosome III from coordinates 50221 to 48653.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24264.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 16 21 + NlaIV GGNNCC 18 18 . . 16 21 + HgiCI* GGYRCC 16 20 . . 19 23 - BceAI ACGGC 4 6 . . 29 32 + FatI CATG 28 32 . . 29 32 + NlaIII CATG 32 28 . . 29 32 + CviAII CATG 29 31 . . 32 36 + BmgT120I GGNCC 33 34 . . 32 36 + AvaII GGWCC 32 35 . . 32 36 + AsuI* GGNCC 32 35 . . 35 38 + MnlI CCTC 45 44 . . 42 46 + BlsI GCNGC 44 43 . . 42 46 + BisI GCNGC 43 44 . . 42 46 + TseI GCWGC 42 45 . . 40 45 - EcoP15I CAGCAG 12 14 . . 45 50 + Cac8I GCNNGC 47 47 . . 42 46 - BbvI GCAGC 29 33 . . 50 53 + MnlI CCTC 60 59 . . 53 56 + MnlI CCTC 63 62 . . 51 56 - BseRI GAGGAG 42 40 . . 66 76 + BsiYI* CCNNNNNNNGG 72 69 . . 75 78 + CviJI RGCY 76 76 . . 73 76 - MnlI CCTC 66 65 . . 81 84 + CviJI RGCY 82 82 . . 81 84 + HaeIII GGCC 82 82 . . 81 86 + NlaIV GGNNCC 83 83 . . 81 85 + BmgT120I GGNCC 82 83 . . 81 85 + AsuI* GGNCC 81 84 . . 86 91 + Eco57MI CTGRAG 107 105 . . 86 91 + Eco57I CTGAAG 107 105 . . 88 92 + MboII GAAGA 100 99 . . 88 93 + BbvII* GAAGAC 95 99 . . 91 95 + HinfI GANTC 91 94 . . 91 102 + DrdI GACNNNNNNGTC 97 95 . . 91 95 - PleI GAGTC 85 86 . . 95 100 + NspBII* CMGCKG 97 97 . . 91 95 - MlyI GAGTC 85 85 . . 95 98 + AciI CCGC 95 97 . . 104 107 + MseI TTAA 104 106 . . 110 115 + BalI TGGCCA 112 112 . . 110 115 + CfrI YGGCCR 110 114 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + HaeIII GGCC 112 112 . . 118 122 + HinfI GANTC 118 121 . . 118 122 - PleI GAGTC 112 113 . . 118 122 - MlyI GAGTC 112 112 . . 122 126 + Hin4II* CCTTC 132 131 . . 129 140 + BdaI TGANNNNNNTCA 118 116 152 150 131 136 + TatI WGTACW 131 135 . . 132 135 + Csp6I GTAC 132 134 . . 132 135 + RsaI GTAC 133 133 . . 129 140 - BdaI TGANNNNNNTCA 152 150 118 116 156 161 + Cac8I GCNNGC 158 158 . . 160 163 - AciI CCGC 160 162 . . 175 180 + DsaI* CCRYGG 175 179 . . 175 180 + StyI CCWWGG 175 179 . . 175 180 + NcoI CCATGG 175 179 . . 175 180 + SecI* CCNNGG 175 179 . . 175 185 + BsiYI* CCNNNNNNNGG 181 178 . . 175 185 + PflMI CCANNNNNTGG 181 178 . . 176 179 + FatI CATG 175 179 . . 176 179 + NlaIII CATG 179 175 . . 176 179 + CviAII CATG 176 178 . . 183 188 + BalI TGGCCA 185 185 . . 183 188 + CfrI YGGCCR 183 187 . . 184 187 + CviJI RGCY 185 185 . . 184 187 + HaeIII GGCC 185 185 . . 187 193 + TspRI CASTGNN 193 184 . . 188 192 + Tsp4CI* ACNGT 190 189 . . 198 201 + FatI CATG 197 201 . . 198 201 + NlaIII CATG 201 197 . . 198 201 + CviAII CATG 198 200 . . 201 204 + CviJI RGCY 202 202 . . 201 206 + NlaIV GGNNCC 203 203 . . 204 209 + Hpy178III* TCNNGA 205 207 . . 212 215 + TaiI ACGT 215 211 . . 212 215 + SetI ASST 215 211 . . 212 215 + MaeII ACGT 212 214 . . 215 218 + MseI TTAA 215 217 . . 219 222 + CviJI RGCY 220 220 . . 220 224 + TauI GCSGC 223 220 . . 220 224 + BlsI GCNGC 222 221 . . 220 224 + BisI GCNGC 221 222 . . 221 224 + AciI CCGC 221 223 . . 223 228 + NmeAIII GCCGAG 249 247 . . 226 230 - BsmAI GTCTC 220 224 . . 250 253 + SetI ASST 253 249 . . 251 256 + StyI CCWWGG 251 255 . . 251 256 + SecI* CCNNGG 251 255 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + HaeIII GGCC 256 256 . . 255 259 + BmgT120I GGNCC 256 257 . . 255 259 + AsuI* GGNCC 255 258 . . 261 264 + DpnI GATC 262 262 . . 261 264 + BstKTI GATC 263 261 . . 261 264 + MboI GATC 260 264 . . 263 266 + TaqI TCGA 263 265 . . 266 270 + Tsp4CI* ACNGT 268 267 . . 268 273 + TatI WGTACW 268 272 . . 269 272 + Csp6I GTAC 269 271 . . 269 272 + RsaI GTAC 270 270 . . 279 283 + BssKI CCNGG 278 283 . . 279 283 + BseBI CCWGG 280 281 . . 279 283 + ScrFI CCNGG 280 281 . . 279 283 + EcoRII CCWGG 278 283 . . 280 288 + AlwNI CAGNNNCTG 285 282 . . 282 287 + XhoII RGATCY 282 286 . . 282 286 + BinI* GGATC 290 291 . . 283 286 + DpnI GATC 284 284 . . 283 286 + BstKTI GATC 285 283 . . 283 286 + MboI GATC 282 286 . . 307 311 + BssKI CCNGG 306 311 . . 307 311 + BseBI CCWGG 308 309 . . 307 312 + SecI* CCNNGG 307 311 . . 307 311 + ScrFI CCNGG 308 309 . . 307 311 + EcoRII CCWGG 306 311 . . 311 316 + NlaIV GGNNCC 313 313 . . 318 323 + HindIII AAGCTT 318 322 . . 319 322 + SetI ASST 322 318 . . 319 322 + CviJI RGCY 320 320 . . 319 322 + AluI AGCT 320 320 . . 323 334 + BdaI TGANNNNNNTCA 312 310 346 344 324 328 + MboII GAAGA 336 335 . . 324 333 + XmnI GAANNNNTTC 328 328 . . 323 334 - BdaI TGANNNNNNTCA 346 344 312 310 341 346 + BtgZI GCGATG 356 360 . . 346 351 + Hpy166II GTNNAC 348 348 . . 346 351 + HpaI GTTAAC 348 348 . . 346 351 + HindII GTYRAC 348 348 . . 347 350 + MseI TTAA 347 349 . . 355 358 + TaqI TCGA 355 357 . . 356 361 + McrI* CGRYCG 359 357 . . 356 361 + PvuI CGATCG 359 357 . . 357 360 + DpnI GATC 358 358 . . 357 360 + BstKTI GATC 359 357 . . 357 360 + MboI GATC 356 360 . . 358 363 + ClaI ATCGAT 359 361 . . 359 362 + TaqI TCGA 359 361 . . 369 375 + PpuMI RGGWCCY 370 373 . . 369 375 + DraII RGGNCCY 370 373 . . 369 373 + BslFI GGGAC 383 387 . . 369 374 + NlaIV GGNNCC 371 371 . . 367 370 - MnlI CCTC 360 359 . . 370 374 + BmgT120I GGNCC 371 372 . . 370 374 + AvaII GGWCC 370 373 . . 370 374 + AsuI* GGNCC 370 373 . . 372 375 + SetI ASST 375 371 . . 374 377 + MaeI CTAG 374 376 . . 382 387 + NmeAIII GCCGAG 408 406 . . 383 388 + SecI* CCNNGG 383 387 . . 387 390 + CviJI RGCY 388 388 . . 387 390 + HaeIII GGCC 388 388 . . 385 388 - MnlI CCTC 378 377 . . 398 401 + TspEI AATT 397 401 . . 401 406 + Hpy178III* TCNNGA 402 404 . . 401 406 + BspHI TCATGA 401 405 . . 402 405 + FatI CATG 401 405 . . 402 405 + NlaIII CATG 405 401 . . 402 405 + CviAII CATG 402 404 . . 400 404 - TspDTI ATGAA 390 388 . . 404 409 + BclI TGATCA 404 408 . . 405 408 + DpnI GATC 406 406 . . 405 408 + BstKTI GATC 407 405 . . 405 408 + MboI GATC 404 408 . . 415 418 + CviJI RGCY 416 416 . . 420 425 + Cfr10I RCCGGY 420 424 . . 421 424 + HpaII CCGG 421 423 . . 423 426 + CviJI RGCY 424 424 . . 430 440 + MwoI GCNNNNNNNGC 436 433 . . 430 434 - BceAI ACGGC 415 417 . . 442 445 + DpnI GATC 443 443 . . 442 445 + BstKTI GATC 444 442 . . 442 445 + MboI GATC 441 445 . . 450 453 + SetI ASST 453 449 . . 450 453 + CviJI RGCY 451 451 . . 450 453 + AluI AGCT 451 451 . . 451 461 + MwoI GCNNNNNNNGC 457 454 . . 455 458 + SetI ASST 458 454 . . 468 477 + PshAI GACNNNNGTC 472 472 . . 466 470 - BsmAI GTCTC 460 464 . . 475 479 + Tsp45I GTSAC 474 479 . . 475 479 + MaeIII GTNAC 474 479 . . 479 484 - Eco57MI CTGRAG 464 462 . . 479 484 - GsuI CTGGAG 464 462 . . 481 485 - BsrI ACTGG 481 479 . . 496 501 + BetI* WCCGGW 496 500 . . 497 500 + HpaII CCGG 497 499 . . 508 512 + HgaI GACGC 517 522 . . 508 513 + AcyI GRCGYC 509 511 . . 526 529 + SetI ASST 529 525 . . 536 541 + DsaI* CCRYGG 536 540 . . 536 541 + StyI CCWWGG 536 540 . . 536 541 + NcoI CCATGG 536 540 . . 536 541 + SecI* CCNNGG 536 540 . . 537 540 + FatI CATG 536 540 . . 537 540 + NlaIII CATG 540 536 . . 537 540 + CviAII CATG 537 539 . . 539 544 + BalI TGGCCA 541 541 . . 539 544 + CfrI YGGCCR 539 543 . . 540 543 + CviJI RGCY 541 541 . . 540 543 + HaeIII GGCC 541 541 . . 542 555 - AjuI GAANNNNNNNTTGG 567 562 535 530 565 575 + Hin4I GAYNNNNNVTC 556 551 588 583 565 573 + Tth111I GACNNNGTC 568 569 . . 566 576 + BsaXI ACNNNNNCTCC 556 553 586 583 571 575 + BsmAI GTCTC 576 580 . . 575 580 + NspBII* CMGCKG 577 577 . . 575 578 + AciI CCGC 575 577 . . 577 587 + MwoI GCNNNNNNNGC 583 580 . . 601 606 + HindIII AAGCTT 601 605 . . 602 605 + SetI ASST 605 601 . . 602 605 + CviJI RGCY 603 603 . . 602 605 + AluI AGCT 603 603 . . 614 624 + BsaXI ACNNNNNCTCC 604 601 634 631 620 623 + MnlI CCTC 630 629 . . 623 626 + AciI CCGC 623 625 . . 626 631 + DsaI* CCRYGG 626 630 . . 626 631 + StyI CCWWGG 626 630 . . 626 631 + NcoI CCATGG 626 630 . . 626 631 + SecI* CCNNGG 626 630 . . 627 630 + FatI CATG 626 630 . . 627 630 + NlaIII CATG 630 626 . . 622 627 - EciI GGCGGA 612 610 . . 627 630 + CviAII CATG 627 629 . . 635 638 + CviJI RGCY 636 636 . . 638 643 + SfeI* CTRYAG 638 642 . . 643 648 + Hpy166II GTNNAC 645 645 . . 643 648 + BssNAI GTATAC 645 645 . . 643 648 + AccI GTMKAC 644 646 . . 650 654 + Tsp4CI* ACNGT 652 651 . . 660 663 + CviJI RGCY 661 661 . . 661 671 + MwoI GCNNNNNNNGC 667 664 . . 664 669 + EcoRV GATATC 666 666 . . 673 677 + HgaI GACGC 682 687 . . 698 701 + CviRI* TGCA 699 699 . . 708 711 + CviJI RGCY 709 709 . . 715 725 + BsiYI* CCNNNNNNNGG 721 718 . . 724 728 + HphI GGTGA 736 735 . . 731 734 + TaqI TCGA 731 733 . . 732 736 + Hpy99I CGWCG 736 731 . . 734 738 + Tsp4CI* ACNGT 736 735 . . 736 741 + NlaIV GGNNCC 738 738 . . 739 743 - TspGWI ACGGA 729 727 . . 755 758 + TaiI ACGT 758 754 . . 755 758 + SetI ASST 758 754 . . 755 758 + MaeII ACGT 755 757 . . 756 760 + Hpy99I CGWCG 760 755 . . 758 761 + TaqI TCGA 758 760 . . 764 767 - AciI CCGC 764 766 . . 779 783 + MaeIII GTNAC 778 783 . . 799 802 - MnlI CCTC 792 791 . . 802 806 + MboII GAAGA 814 813 . . 806 809 + TspEI AATT 805 809 . . 811 815 + MboII GAAGA 823 822 . . 820 830 + FalI AAGNNNNNCTT 811 806 843 838 821 824 + CviJI RGCY 822 822 . . 823 826 + MnlI CCTC 833 832 . . 820 830 - FalI AAGNNNNNCTT 843 838 811 806 839 844 + BalI TGGCCA 841 841 . . 839 844 + CfrI YGGCCR 839 843 . . 840 843 + CviJI RGCY 841 841 . . 840 843 + HaeIII GGCC 841 841 . . 855 858 + SetI ASST 858 854 . . 854 857 - MnlI CCTC 847 846 . . 862 866 + TspDTI ATGAA 877 875 . . 875 879 + SfaNI GCATC 884 888 . . 877 882 + ClaI ATCGAT 878 880 . . 878 881 + TaqI TCGA 878 880 . . 880 884 - SfaNI GCATC 870 874 . . 889 894 + ApoI RAATTY 889 893 . . 890 893 + TspEI AATT 889 893 . . 904 909 + NaeI GCCGGC 906 906 . . 904 909 + MroNI GCCGGC 904 908 . . 904 909 + Cac8I GCNNGC 906 906 . . 904 909 + Cfr10I RCCGGY 904 908 . . 905 908 + HpaII CCGG 905 907 . . 918 921 + FatI CATG 917 921 . . 918 921 + NlaIII CATG 921 917 . . 918 921 + CviAII CATG 918 920 . . 919 923 + TspDTI ATGAA 934 932 . . 921 925 - Hin4II* CCTTC 915 914 . . 929 932 + TspEI AATT 928 932 . . 934 937 + MnlI CCTC 944 943 . . 944 948 + BccI CCATC 952 953 . . 945 949 - FokI GGATG 931 935 . . 945 949 - BseGI GGATG 944 942 . . 954 957 + FatI CATG 953 957 . . 954 957 + NlaIII CATG 957 953 . . 954 957 + CviAII CATG 954 956 . . 959 964 + Eco57MI CTGRAG 980 978 . . 959 964 + Eco57I CTGAAG 980 978 . . 961 965 + MboII GAAGA 973 972 . . 961 966 + BbvII* GAAGAC 968 972 . . 972 975 + Csp6I GTAC 972 974 . . 972 975 + RsaI GTAC 973 973 . . 974 978 + Tsp4CI* ACNGT 976 975 . . 982 985 + MnlI CCTC 992 991 . . 991 995 + Hpy188I TCNGA 993 992 . . 992 997 + Eco57MI CTGRAG 1013 1011 . . 992 997 + Eco57I CTGAAG 1013 1011 . . 994 998 + MboII GAAGA 1006 1005 . . 994 999 - Ksp632I* CTCTTC 989 992 . . 997 1000 - MnlI CCTC 990 989 . . 1010 1013 + TspEI AATT 1009 1013 . . 1023 1026 + DpnI GATC 1024 1024 . . 1023 1026 + BstKTI GATC 1025 1023 . . 1023 1026 + MboI GATC 1022 1026 . . 1033 1037 + PleI GAGTC 1041 1042 . . 1033 1037 + MlyI GAGTC 1042 1042 . . 1033 1037 + HinfI GANTC 1033 1036 . . 1029 1034 - MmeI TCCRAC 1010 1008 . . 1037 1041 + DdeI CTNAG 1037 1040 . . 1041 1044 + CviJI RGCY 1042 1042 . . 1048 1052 + TfiI GAWTC 1048 1051 . . 1048 1052 + HinfI GANTC 1048 1051 . . 1058 1061 + MseI TTAA 1058 1060 . . 1055 1060 - TsoI TARCCA 1045 1043 . . 1060 1070 + FalI AAGNNNNNCTT 1051 1046 1083 1078 1067 1072 + Hpy178III* TCNNGA 1068 1070 . . 1060 1070 - FalI AAGNNNNNCTT 1083 1078 1051 1046 1074 1077 + SetI ASST 1077 1073 . . 1075 1079 + HphI GGTGA 1087 1086 . . 1082 1085 + MnlI CCTC 1092 1091 . . 1078 1082 - SfaNI GCATC 1068 1072 . . 1091 1096 + Hpy178III* TCNNGA 1092 1094 . . 1098 1102 - BslFI GGGAC 1083 1087 . . 1106 1111 + XhoII RGATCY 1106 1110 . . 1106 1111 + BglII AGATCT 1106 1110 . . 1107 1110 + DpnI GATC 1108 1108 . . 1107 1110 + BstKTI GATC 1109 1107 . . 1107 1110 + MboI GATC 1106 1110 . . 1112 1115 + TaqI TCGA 1112 1114 . . 1112 1117 + Hpy178III* TCNNGA 1113 1115 . . 1109 1113 - MboII GAAGA 1101 1100 . . 1116 1128 + AloI GAACNNNNNNTCC 1108 1103 1140 1135 1123 1127 + TfiI GAWTC 1123 1126 . . 1123 1127 + HinfI GANTC 1123 1126 . . 1127 1130 + MnlI CCTC 1137 1136 . . 1133 1137 - MboII GAAGA 1125 1124 . . 1132 1137 - BbvII* GAAGAC 1125 1129 . . 1138 1143 + MfeI CAATTG 1138 1142 . . 1139 1142 + TspEI AATT 1138 1142 . . 1156 1159 + FatI CATG 1155 1159 . . 1156 1159 + NlaIII CATG 1159 1155 . . 1156 1159 + CviAII CATG 1156 1158 . . 1159 1170 + DrdI GACNNNNNNGTC 1165 1163 . . 1168 1173 + Hpy166II GTNNAC 1170 1170 . . 1168 1173 + HindII GTYRAC 1170 1170 . . 1176 1186 + BsiYI* CCNNNNNNNGG 1182 1179 . . 1182 1186 - Hin4II* CCTTC 1176 1175 . . 1187 1190 + TaiI ACGT 1190 1186 . . 1187 1190 + SetI ASST 1190 1186 . . 1187 1190 + MaeII ACGT 1187 1189 . . 1199 1204 + TatI WGTACW 1199 1203 . . 1200 1203 + Csp6I GTAC 1200 1202 . . 1200 1203 + RsaI GTAC 1201 1201 . . 1210 1215 + DsaI* CCRYGG 1210 1214 . . 1210 1215 + StyI CCWWGG 1210 1214 . . 1210 1215 + NcoI CCATGG 1210 1214 . . 1210 1215 + SecI* CCNNGG 1210 1214 . . 1210 1220 + BsiYI* CCNNNNNNNGG 1216 1213 . . 1210 1220 + PflMI CCANNNNNTGG 1216 1213 . . 1211 1214 + FatI CATG 1210 1214 . . 1211 1214 + NlaIII CATG 1214 1210 . . 1211 1214 + CviAII CATG 1211 1213 . . 1220 1224 + Tsp45I GTSAC 1219 1224 . . 1220 1224 + MaeIII GTNAC 1219 1224 . . 1222 1228 + TspRI CASTGNN 1228 1219 . . 1223 1227 + Tsp4CI* ACNGT 1225 1224 . . 1236 1239 + CviJI RGCY 1237 1237 . . 1236 1239 + HaeIII GGCC 1237 1237 . . 1236 1241 + NlaIV GGNNCC 1238 1238 . . 1236 1240 + BmgT120I GGNCC 1237 1238 . . 1236 1240 + AsuI* GGNCC 1236 1239 . . 1255 1258 + MaeI CTAG 1255 1257 . . 1257 1260 + SetI ASST 1260 1256 . . 1257 1260 + CviJI RGCY 1258 1258 . . 1257 1260 + AluI AGCT 1258 1258 . . 1264 1267 + SetI ASST 1267 1263 . . 1282 1287 + MmeI TCCRAC 1307 1305 . . 1282 1286 + Hpy188I TCNGA 1284 1283 . . 1284 1288 + Hpy99I CGWCG 1288 1283 . . 1280 1284 - FokI GGATG 1266 1270 . . 1280 1284 - BseGI GGATG 1279 1277 . . 1286 1289 + TaiI ACGT 1289 1285 . . 1286 1289 + SetI ASST 1289 1285 . . 1286 1289 + MaeII ACGT 1286 1288 . . 1303 1306 + MaeI CTAG 1303 1305 . . 1311 1317 + TspRI CASTGNN 1317 1308 . . 1325 1329 + Hpy188I TCNGA 1327 1326 . . 1330 1335 + AcyI GRCGYC 1331 1333 . . 1328 1331 - MnlI CCTC 1321 1320 . . 1332 1336 + Hpy99I CGWCG 1336 1331 . . 1331 1335 - HgaI GACGC 1320 1325 . . 1338 1341 + TspEI AATT 1337 1341 . . 1344 1347 + SetI ASST 1347 1343 . . 1345 1351 + BstEII GGTNACC 1345 1350 . . 1346 1350 + MaeIII GTNAC 1345 1350 . . 1342 1346 - Hin4II* CCTTC 1336 1335 . . 1368 1373 + SecI* CCNNGG 1368 1372 . . 1369 1373 + BssKI CCNGG 1368 1373 . . 1369 1373 + BseBI CCWGG 1370 1371 . . 1369 1373 + ScrFI CCNGG 1370 1371 . . 1369 1373 + EcoRII CCWGG 1368 1373 . . 1371 1374 + SetI ASST 1374 1370 . . 1384 1388 + Hpy188I TCNGA 1386 1385 . . 1387 1391 + TfiI GAWTC 1387 1390 . . 1387 1391 + HinfI GANTC 1387 1390 . . 1396 1401 + Hpy166II GTNNAC 1398 1398 . . 1398 1401 + Csp6I GTAC 1398 1400 . . 1398 1401 + RsaI GTAC 1399 1399 . . 1401 1406 + StyI CCWWGG 1401 1405 . . 1401 1406 + SecI* CCNNGG 1401 1405 . . 1404 1407 + SetI ASST 1407 1403 . . 1408 1413 + Hpy178III* TCNNGA 1409 1411 . . 1411 1416 + XhoII RGATCY 1411 1415 . . 1412 1415 + DpnI GATC 1413 1413 . . 1412 1415 + BstKTI GATC 1414 1412 . . 1412 1415 + MboI GATC 1411 1415 . . 1415 1420 + StyI CCWWGG 1415 1419 . . 1415 1420 + SecI* CCNNGG 1415 1419 . . 1412 1416 - BinI* GGATC 1406 1407 . . 1420 1424 + HinfI GANTC 1420 1423 . . 1420 1424 - PleI GAGTC 1414 1415 . . 1420 1424 - MlyI GAGTC 1414 1414 . . 1430 1433 + TaqI TCGA 1430 1432 . . 1435 1439 - TspDTI ATGAA 1425 1423 . . 1448 1452 + Tsp4CI* ACNGT 1450 1449 . . 1451 1455 + Tsp45I GTSAC 1450 1455 . . 1451 1455 + MaeIII GTNAC 1450 1455 . . 1457 1460 + TaqI TCGA 1457 1459 . . 1458 1462 + Hpy99I CGWCG 1462 1457 . . 1459 1464 + ZraI GACGTC 1461 1461 . . 1459 1464 + AcyI GRCGYC 1460 1462 . . 1459 1464 + AatII GACGTC 1463 1459 . . 1460 1463 + TaiI ACGT 1463 1459 . . 1460 1463 + SetI ASST 1463 1459 . . 1460 1463 + MaeII ACGT 1460 1462 . . 1461 1468 + SgrDI CGTCGACG 1462 1466 . . 1461 1465 + Hpy99I CGWCG 1465 1460 . . 1462 1467 + SalI GTCGAC 1462 1466 . . 1462 1467 + Hpy166II GTNNAC 1464 1464 . . 1462 1467 + HindII GTYRAC 1464 1464 . . 1462 1467 + AccI GTMKAC 1463 1465 . . 1463 1466 + TaqI TCGA 1463 1465 . . 1464 1468 + Hpy99I CGWCG 1468 1463 . . 1466 1470 + Tsp4CI* ACNGT 1468 1467 . . 1472 1477 + StuI AGGCCT 1474 1474 . . 1473 1476 + CviJI RGCY 1474 1474 . . 1473 1476 + HaeIII GGCC 1474 1474 . . 1479 1482 + Csp6I GTAC 1479 1481 . . 1479 1482 + RsaI GTAC 1480 1480 . . 1483 1487 + MboII GAAGA 1495 1494 . . 1488 1491 + CviJI RGCY 1489 1489 . . 1490 1495 + SecI* CCNNGG 1490 1494 . . 1491 1495 + BssKI CCNGG 1490 1495 . . 1491 1495 + BseBI CCWGG 1492 1493 . . 1491 1495 + ScrFI CCNGG 1492 1493 . . 1491 1495 + EcoRII CCWGG 1490 1495 . . 1500 1503 + SetI ASST 1503 1499 . . 1500 1503 + CviJI RGCY 1501 1501 . . 1500 1503 + AluI AGCT 1501 1501 . . 1501 1505 + BlsI GCNGC 1503 1502 . . 1501 1505 + BisI GCNGC 1502 1503 . . 1501 1505 + TseI GCWGC 1501 1504 . . 1504 1514 + MwoI GCNNNNNNNGC 1510 1507 . . 1505 1509 + DdeI CTNAG 1505 1508 . . 1501 1505 - BbvI GCAGC 1488 1492 . . 1502 1507 - EcoP15I CAGCAG 1474 1476 . . 1505 1509 - BspCNI CTCAG 1497 1495 . . 1505 1509 - BseMII CTCAG 1496 1494 . . 1507 1510 - MnlI CCTC 1500 1499 . . 1504 1510 - Bpu10I CCTNAGC 1505 1508 . . 1504 1510 - BbvCI CCTCAGC 1505 1508 . . 1512 1515 + CviJI RGCY 1513 1513 . . 1516 1520 - SfaNI GCATC 1506 1510 . . 1522 1526 + HgaI GACGC 1531 1536 . . 1525 1535 + MwoI GCNNNNNNNGC 1531 1528 . . 1529 1532 + TspEI AATT 1528 1532 . . 1533 1536 + CviJI RGCY 1534 1534 . . 1539 1550 + BcgI CGANNNNNNTGC 1528 1526 1562 1560 1540 1544 + MboII GAAGA 1552 1551 . . 1543 1547 + MboII GAAGA 1555 1554 . . 1546 1550 - SfaNI GCATC 1536 1540 . . 1554 1559 + Hpy178III* TCNNGA 1555 1557 . . 1562 1565 + TspEI AATT 1561 1565 . . #--------------------------------------- # # # AatII: # [GACGTC] # 106 1463 # # AccI: # [GTMKAC] # 106 644 819 # # AciI: # [CCGC] # 48 61 65 95 141 354 # 805 # # AcyI: # [GRCGYC] # 109 129 509 822 # # AjuI: # [GAANNNNNNNTTGG] # 32 535 1002 # # AloI: # [GAACNNNNNNTCC] # 32 429 1108 # # AluI: # [AGCT] # 68 131 152 243 320 655 # # AlwNI: # [CAGNNNCTG] # 285 1284 # # ApoI: # [RAATTY] # 680 889 # # AsuI*: # [GGNCC] # 32 49 115 174 333 866 # # AvaII: # [GGWCC] # 32 338 1199 # # BalI: # [TGGCCA] # 73 112 300 356 728 # # BbvCI: # [CCTCAGC] # 64 1505 # # BbvI: # [GCAGC] # 29 81 1459 # # BbvII*: # [GAAGAC] # 95 157 444 873 # # BccI: # [CCATC] # 617 952 # # BceAI: # [ACGGC] # 4 411 1154 # # BcgI: # [CGANNNNNNTGC] # 7 34 1528 # # BclI: # [TGATCA] # 404 1165 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 118 160 1223 # # BetI*: # [WCCGGW] # 496 1073 # # BglII: # [AGATCT] # 463 1106 # # BinI*: # [GGATC] # 163 290 1116 # # BisI: # [GCNGC] # 43 67 178 1281 # # BlsI: # [GCNGC] # 44 66 178 1281 # # BmgT120I: # [GGNCC] # 33 49 115 174 332 866 # # Bpu10I: # [CCTNAGC] # 64 1505 # # BsaXI: # [ACNNNNNCTCC] # 18 30 30 556 935 # # BseBI: # [CCWGG] # 28 77 122 280 1062 # # BseGI: # [GGATG] # 290 335 944 # # BseMII: # [CTCAG] # 73 1496 # # BseRI: # [GAGGAG] # 42 1527 # # BsiYI*: # [CCNNNNNNNGG] # 34 72 109 353 461 540 # # BslFI: # [GGGAC] # 383 486 700 # # BsmAI: # [GTCTC] # 116 220 240 993 # # BspCNI: # [CTCAG] # 72 1497 # # BspHI: # [TCATGA] # 401 1168 # # BsrI: # [ACTGG] # 481 1088 # # BssKI: # [CCNGG] # 28 79 122 278 1062 # # BssNAI: # [GTATAC] # 645 924 # # BstEII: # [GGTNACC] # 224 1345 # # BstKTI: # [GATC] # 22 37 48 74 84 155 # 263 305 581 # # BtgZI: # [GCGATG] # 356 1213 # # Cac8I: # [GCNNGC] # 47 111 663 748 # # Cfr10I: # [RCCGGY] # 420 484 665 # # CfrI: # [YGGCCR] # 73 110 300 356 730 # # ClaI: # [ATCGAT] # 359 519 691 # # Csp6I: # [GTAC] # 81 90 132 137 198 228 # 703 # # CviAII: # [CATG] # 22 29 36 55 90 135 # 147 202 204 291 358 # # CviJI: # [RGCY] # 6 8 12 12 15 17 # 18 19 21 21 25 27 # 28 30 33 35 36 48 # 62 64 68 73 76 90 # 113 195 201 216 # # CviRI*: # [TGCA] # 699 870 # # DdeI: # [CTNAG] # 64 468 1037 # # DpnI: # [GATC] # 22 37 48 74 84 156 # 262 305 581 # # DraII: # [RGGNCCY] # 370 1199 # # DrdI: # [GACNNNNNNGTC] # 97 404 1068 # # DsaI*: # [CCRYGG] # 90 175 359 361 584 # # EciI: # [GGCGGA] # 612 957 # # Eco57I: # [CTGAAG] # 33 107 556 873 # # Eco57MI: # [CTGRAG] # 33 107 357 516 556 # # EcoP15I: # [CAGCAG] # 12 95 1462 # # EcoRII: # [CCWGG] # 28 79 122 278 1062 # # EcoRV: # [GATATC] # 666 903 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 208 486 811 # # FatI: # [CATG] # 22 28 36 55 90 135 # 147 202 204 291 359 # # FokI: # [GGATG] # 303 335 931 # # GsuI: # [CTGGAG] # 464 1105 # # HaeIII: # [GGCC] # 30 71 73 82 95 132 # 153 237 300 396 # # HgaI: # [GACGC] # 38 165 211 517 638 # # HgiCI*: # [GGYRCC] # 16 1553 # # Hin4I: # [GAYNNNNNVTC] # 32 556 981 # # Hin4II*: # [CCTTC] # 132 160 233 261 783 # # HindII: # [GTYRAC] # 105 294 348 822 # # HindIII: # [AAGCTT] # 283 318 968 # # HinfI: # [GANTC] # 15 27 33 75 91 149 # 264 915 # # HpaI: # [GTTAAC] # 348 1221 # # HpaII: # [CCGG] # 76 408 421 664 # # HphI: # [GGTGA] # 351 482 736 # # Hpy166II: # [GTNNAC] # 66 105 228 297 348 525 # # Hpy178III*: # [TCNNGA] # 14 21 24 146 197 205 # 296 666 # # Hpy188I: # [TCNGA] # 43 59 183 291 993 # # Hpy99I: # [CGWCG] # 3 3 24 48 101 126 # 528 736 # # Ksp632I*: # [CTCTTC] # 580 989 # # MaeI: # [CTAG] # 48 266 374 881 # # MaeII: # [ACGT] # 99 109 174 212 432 543 # # MaeIII: # [GTNAC] # 105 119 126 304 441 474 # # MboI: # [GATC] # 22 37 48 74 84 158 # 260 305 581 # # MboII: # [GAAGA] # 3 9 14 24 33 57 # 95 100 150 236 370 478 # # McrI*: # [CGRYCG] # 359 1210 # # MfeI: # [CAATTG] # 431 1138 # # MlyI: # [GAGTC] # 27 85 155 372 930 # # MmeI: # [TCCRAC] # 262 297 1010 # # MnlI: # [CCTC] # 2 3 3 14 15 18 # 41 45 45 46 69 97 # 100 162 179 184 252 294 # # MroNI: # [GCCGGC] # 665 904 # # MseI: # [TTAA] # 104 111 132 511 711 # # MwoI: # [GCNNNNNNNGC] # 21 21 38 84 126 436 # 843 # # NaeI: # [GCCGGC] # 663 906 # # NcoI: # [CCATGG] # 90 175 359 361 584 # # NlaIII: # [CATG] # 22 32 36 55 90 135 # 147 202 204 291 355 # # NlaIV: # [GGNNCC] # 18 58 65 110 120 331 # 367 500 # # NmeAIII: # [GCCGAG] # 159 249 1161 # # NspBII*: # [CMGCKG] # 97 480 992 # # PflMI: # [CCANNNNNTGG] # 181 353 1035 # # PleI: # [GAGTC] # 27 85 155 373 929 # # PpuMI: # [RGGWCCY] # 370 1199 # # PshAI: # [GACNNNNGTC] # 472 1097 # # PvuI: # [CGATCG] # 359 1210 # # RsaI: # [GTAC] # 81 89 133 137 198 228 # 703 # # SalI: # [GTCGAC] # 107 1462 # # ScrFI: # [CCNGG] # 28 77 122 280 1062 # # SecI*: # [CCNNGG] # 14 33 56 75 76 76 # 79 90 153 158 175 584 # # SetI: # [ASST] # 5 7 22 27 33 38 # 40 53 56 58 66 69 # 70 71 76 78 100 113 # 153 215 219 # # SfaNI: # [GCATC] # 14 30 33 184 438 870 # # SfeI*: # [CTRYAG] # 638 931 # # SgrDI: # [CGTCGACG] # 107 1462 # # StuI: # [AGGCCT] # 95 1474 # # StyI: # [CCWWGG] # 14 76 90 154 175 191 # 285 584 # # TaiI: # [ACGT] # 99 106 174 215 432 543 # # TaqI: # [TCGA] # 4 6 27 27 92 106 # 120 234 263 318 372 # # TatI: # [WGTACW] # 131 137 370 931 # # TauI: # [GCSGC] # 223 1346 # # TfiI: # [GAWTC] # 75 182 264 1048 # # TseI: # [GCWGC] # 42 68 1459 # # TsoI: # [TARCCA] # 524 1045 # # Tsp45I: # [GTSAC] # 119 231 474 745 # # Tsp4CI*: # [ACNGT] # 18 78 84 101 190 225 # 240 249 384 # # TspDTI: # [ATGAA] # 16 57 144 374 487 491 # # TspEI: # [AATT] # 8 33 39 81 84 129 # 191 199 397 408 # # TspGWI: # [ACGGA] # 729 840 # # TspRI: # [CASTGNN] # 89 193 252 1035 # # Tth111I: # [GACNNNGTC] # 568 1001 # # XhoII: # [RGATCY] # 158 282 305 824 # # XmnI: # [GAANNNNTTC] # 328 1241 # # ZraI: # [GACGTC] # 108 1461 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1569 # Reported_sequences: 1 # Reported_hitcount: 486 #---------------------------------------