Restriction Fragments for HIS4/YCL030C



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The currently selected gene/sequence is :

HIS4/YCL030C on chromosome III from coordinates 68333 to 65934.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.12531.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 12 16 + TfiI GAWTC 12 15 . . 12 16 + HinfI GANTC 12 15 . . 18 22 + Tsp4CI* ACNGT 20 19 . . 22 25 + MseI TTAA 22 24 . . 24 27 + TspEI AATT 23 27 . . 31 41 + Hin4I GAYNNNNNVTC 22 17 54 49 31 34 + DpnI GATC 32 32 . . 31 34 + BstKTI GATC 33 31 . . 31 34 + MboI GATC 30 34 . . 36 39 + CviJI RGCY 37 37 . . 36 39 + HaeIII GGCC 37 37 . . 38 41 + MnlI CCTC 48 47 . . 41 44 + FatI CATG 40 44 . . 41 44 + NlaIII CATG 44 40 . . 41 44 + CviAII CATG 41 43 . . 54 58 - Hin4II* CCTTC 48 47 . . 62 65 + TaiI ACGT 65 61 . . 62 65 + SetI ASST 65 61 . . 62 65 + MaeII ACGT 62 64 . . 80 83 + SetI ASST 83 79 . . 82 85 + Csp6I GTAC 82 84 . . 82 85 + RsaI GTAC 83 83 . . 90 94 + FokI GGATG 103 107 . . 90 94 + BseGI GGATG 96 94 . . 94 97 + CviJI RGCY 95 95 . . 95 102 + PspXI VCTCGAGB 96 100 . . 91 95 - BccI CCATC 85 86 . . 96 101 + SmlI CTYRAG 96 100 . . 96 101 + XhoI CTCGAG 96 100 . . 96 101 + BmeT110I CYCGRG 97 99 . . 96 101 + AvaI CYCGRG 96 100 . . 97 100 + TaqI TCGA 97 99 . . 100 103 + CviJI RGCY 101 101 . . 103 107 + DdeI CTNAG 103 106 . . 103 107 - BspCNI CTCAG 95 93 . . 103 107 - BseMII CTCAG 94 92 . . 110 114 + TspDTI ATGAA 125 123 . . 112 116 + MboII GAAGA 124 123 . . 112 121 + XmnI GAANNNNTTC 116 116 . . 117 121 + TfiI GAWTC 117 120 . . 117 121 + HinfI GANTC 117 120 . . 112 117 - Ksp632I* CTCTTC 107 110 . . 121 126 - Eco57MI CTGRAG 106 104 . . 121 126 - GsuI CTGGAG 106 104 . . 123 127 - BsrI ACTGG 123 121 . . 136 139 - MnlI CCTC 129 128 . . 139 143 + MboII GAAGA 151 150 . . 139 148 + XmnI GAANNNNTTC 143 143 . . 156 159 + CviJI RGCY 157 157 . . 178 183 + ApoI RAATTY 178 182 . . 179 182 + TspEI AATT 178 182 . . 185 190 + BtgZI GCGATG 200 204 . . 191 195 + TspDTI ATGAA 206 204 . . 203 207 + Hin4II* CCTTC 213 212 . . 198 203 - BsrDI GCAATG 197 195 . . 206 211 + Hpy178III* TCNNGA 207 209 . . 215 219 + TspGWI ACGGA 230 228 . . 223 227 - MboII GAAGA 215 214 . . 232 236 - TspDTI ATGAA 222 220 . . 238 243 + Cac8I GCNNGC 240 240 . . 238 243 + BmtI GCTAGC 242 238 . . 238 243 + NheI GCTAGC 238 242 . . 234 239 - BsrDI GCAATG 233 231 . . 239 242 + MaeI CTAG 239 241 . . 240 245 + TsoI TARCCA 256 254 . . 241 244 + CviJI RGCY 242 242 . . 250 260 + MwoI GCNNNNNNNGC 256 253 . . 247 251 - SfaNI GCATC 237 241 . . 258 261 + CviJI RGCY 259 259 . . 277 282 + MfeI CAATTG 277 281 . . 278 281 + TspEI AATT 277 281 . . 286 289 + Csp6I GTAC 286 288 . . 286 289 + RsaI GTAC 287 287 . . 310 314 + MboII GAAGA 322 321 . . 310 315 - Ksp632I* CTCTTC 305 308 . . 317 321 + Tsp4CI* ACNGT 319 318 . . 329 343 + XcmI CCANNNNNNNNNTGG 336 335 . . 335 338 + TspEI AATT 334 338 . . 339 342 + FatI CATG 338 342 . . 339 342 + NlaIII CATG 342 338 . . 339 342 + CviAII CATG 339 341 . . 337 341 - TspDTI ATGAA 327 325 . . 352 357 + ApoI RAATTY 352 356 . . 353 356 + TspEI AATT 352 356 . . 369 372 + TspEI AATT 368 372 . . 386 389 + TspEI AATT 385 389 . . 388 391 + MseI TTAA 388 390 . . 402 407 + Hpy166II GTNNAC 404 404 . . 402 407 + HindII GTYRAC 404 404 . . 410 420 + FalI AAGNNNNNCTT 401 396 433 428 410 420 - FalI AAGNNNNNCTT 433 428 401 396 421 425 + HphI GGTGA 433 432 . . 427 430 + Csp6I GTAC 427 429 . . 427 430 + RsaI GTAC 428 428 . . 428 433 + SnaBI TACGTA 430 430 . . 428 433 + BsaAI YACGTR 430 430 . . 429 432 + TaiI ACGT 432 428 . . 429 432 + SetI ASST 432 428 . . 429 432 + MaeII ACGT 429 431 . . 431 434 + Csp6I GTAC 431 433 . . 431 434 + RsaI GTAC 432 432 . . 437 441 + Tsp4CI* ACNGT 439 438 . . 441 446 + Hpy178III* TCNNGA 442 444 . . 446 450 + Tsp4CI* ACNGT 448 447 . . 463 466 + MaeI CTAG 463 465 . . 469 474 + SalI GTCGAC 469 473 . . 469 474 + Hpy166II GTNNAC 471 471 . . 469 474 + HindII GTYRAC 471 471 . . 458 469 - CspCI CAANNNNNGTGG 482 480 447 445 469 474 + AccI GTMKAC 470 472 . . 470 473 + TaqI TCGA 470 472 . . 490 493 + MaeI CTAG 490 492 . . 507 512 + AsuII TTCGAA 508 510 . . 508 511 + TaqI TCGA 508 510 . . 505 509 - MboII GAAGA 497 496 . . 510 514 + MboII GAAGA 522 521 . . 518 523 + SfeI* CTRYAG 518 522 . . 528 531 + CviJI RGCY 529 529 . . 528 531 + HaeIII GGCC 529 529 . . 530 541 + BstXI CCANNNNNNTGG 537 533 . . 530 534 + BccI CCATC 538 539 . . 532 537 + ClaI ATCGAT 533 535 . . 533 536 + TaqI TCGA 533 535 . . 566 569 + MaeI CTAG 566 568 . . 572 576 + TspDTI ATGAA 587 585 . . 578 583 + Hpy178III* TCNNGA 579 581 . . 581 585 + BinI* GGATC 589 590 . . 582 585 + DpnI GATC 583 583 . . 582 585 + BstKTI GATC 584 582 . . 582 585 + MboI GATC 581 585 . . 589 593 + HphI GGTGA 601 600 . . 581 592 - TstI CACNNNNNNTCC 605 600 573 568 602 607 + BsrDI GCAATG 609 607 . . 606 611 + BalI TGGCCA 608 608 . . 606 611 + CfrI YGGCCR 606 610 . . 607 610 + CviJI RGCY 608 608 . . 607 610 + HaeIII GGCC 608 608 . . 613 618 + HindIII AAGCTT 613 617 . . 614 617 + SetI ASST 617 613 . . 614 617 + CviJI RGCY 615 615 . . 614 617 + AluI AGCT 615 615 . . 635 639 + Tsp4CI* ACNGT 637 636 . . 640 644 + TfiI GAWTC 640 643 . . 640 644 + HinfI GANTC 640 643 . . 643 647 + Hpy188I TCNGA 645 644 . . 645 649 + FokI GGATG 658 662 . . 645 649 + BseGI GGATG 651 649 . . 646 650 - SfaNI GCATC 636 640 . . 652 655 + MseI TTAA 652 654 . . 676 681 + AclI AACGTT 677 679 . . 677 680 + TaiI ACGT 680 676 . . 677 680 + SetI ASST 680 676 . . 677 680 + MaeII ACGT 677 679 . . 679 684 - MmeI TCCRAC 660 658 . . 699 707 + Tth111I GACNNNGTC 702 703 . . 697 701 - BsmAI GTCTC 691 695 . . 702 705 + FatI CATG 701 705 . . 702 705 + NlaIII CATG 705 701 . . 702 705 + CviAII CATG 702 704 . . 697 702 - Eco31I GGTCTC 691 695 . . 696 706 - BsaXI ACNNNNNCTCC 718 715 688 685 697 707 - Hin4I GAYNNNNNVTC 720 715 688 683 715 719 + HphI GGTGA 727 726 . . 718 723 + EcoRI GAATTC 718 722 . . 718 723 + ApoI RAATTY 718 722 . . 719 722 + TspEI AATT 718 722 . . 727 730 + FatI CATG 726 730 . . 727 730 + NlaIII CATG 730 726 . . 727 730 + CviAII CATG 727 729 . . 740 743 + CviJI RGCY 741 741 . . 742 745 + MaeI CTAG 742 744 . . 745 749 + TfiI GAWTC 745 748 . . 745 749 + HinfI GANTC 745 748 . . 764 767 + CviJI RGCY 765 765 . . 766 771 + SfeI* CTRYAG 766 770 . . 772 776 + HgaI GACGC 781 786 . . 777 782 + Hpy178III* TCNNGA 778 780 . . 776 781 - Eco57MI CTGRAG 761 759 . . 776 781 - GsuI CTGGAG 761 759 . . 784 788 + TfiI GAWTC 784 787 . . 781 784 - MnlI CCTC 774 773 . . 784 788 + HinfI GANTC 784 787 . . 794 797 + MaeI CTAG 794 796 . . 797 801 + MboII GAAGA 809 808 . . 797 802 + BbvII* GAAGAC 804 808 . . 811 815 + HinfI GANTC 811 814 . . 811 815 - PleI GAGTC 805 806 . . 811 815 - MlyI GAGTC 805 805 . . 816 819 + CviRI* TGCA 817 817 . . 826 830 - SfaNI GCATC 816 820 . . 834 837 + DpnI GATC 835 835 . . 834 837 + BstKTI GATC 836 834 . . 834 837 + MboI GATC 833 837 . . 841 845 + MboII GAAGA 853 852 . . 846 849 + SetI ASST 849 845 . . 846 849 + CviJI RGCY 847 847 . . 846 849 + AluI AGCT 847 847 . . 848 853 + Eco57MI CTGRAG 869 867 . . 848 853 + Eco57I CTGAAG 869 867 . . 850 854 + MboII GAAGA 862 861 . . 860 864 + DdeI CTNAG 860 863 . . 860 864 - BspCNI CTCAG 852 850 . . 860 864 - BseMII CTCAG 851 849 . . 862 865 - MnlI CCTC 855 854 . . 876 880 - Hin4II* CCTTC 870 869 . . 881 884 + SetI ASST 884 880 . . 881 884 + CviJI RGCY 882 882 . . 881 884 + AluI AGCT 882 882 . . 894 897 + CviJI RGCY 895 895 . . 892 895 - MnlI CCTC 885 884 . . 895 899 + BlsI GCNGC 897 896 . . 895 899 + BisI GCNGC 896 897 . . 895 899 + TseI GCWGC 895 898 . . 895 899 - BbvI GCAGC 882 886 . . 915 918 + CviRI* TGCA 916 916 . . 917 923 + TspRI CASTGNN 923 914 . . 918 922 + BsrI ACTGG 923 921 . . 920 925 + BalI TGGCCA 922 922 . . 920 925 + CfrI YGGCCR 920 924 . . 921 924 + CviJI RGCY 922 922 . . 921 924 + HaeIII GGCC 922 922 . . 923 934 + BstXI CCANNNNNNTGG 930 926 . . 926 929 + TspEI AATT 925 929 . . 932 937 + BalI TGGCCA 934 934 . . 932 937 + CfrI YGGCCR 932 936 . . 933 936 + CviJI RGCY 934 934 . . 933 936 + HaeIII GGCC 934 934 . . 945 949 - TspDTI ATGAA 935 933 . . 955 960 + ZraI GACGTC 957 957 . . 951 955 - Hin4II* CCTTC 945 944 . . 955 960 + AcyI GRCGYC 956 958 . . 955 960 + AatII GACGTC 959 955 . . 956 959 + TaiI ACGT 959 955 . . 956 959 + SetI ASST 959 955 . . 956 959 + MaeII ACGT 956 958 . . 957 961 + Hpy99I CGWCG 961 956 . . 959 962 + TaqI TCGA 959 961 . . 959 964 + Hpy178III* TCNNGA 960 962 . . 969 973 + Hpy188I TCNGA 971 970 . . 976 980 + TspDTI ATGAA 991 989 . . 981 985 + SfaNI GCATC 990 994 . . 984 988 + Hpy188I TCNGA 986 985 . . 985 990 + Eco57MI CTGRAG 1006 1004 . . 985 990 + Eco57I CTGAAG 1006 1004 . . 989 992 + SetI ASST 992 988 . . 991 995 + MaeIII GTNAC 990 995 . . 987 991 - Hin4II* CCTTC 981 980 . . 999 1003 + TspGWI ACGGA 1014 1012 . . 1005 1008 + SetI ASST 1008 1004 . . 1006 1010 + HphI GGTGA 1018 1017 . . 1012 1018 + EspI* GCTNAGC 1013 1016 . . 1009 1013 - SfaNI GCATC 999 1003 . . 1013 1017 + DdeI CTNAG 1013 1016 . . 1016 1019 + CviJI RGCY 1017 1017 . . 1027 1032 - MmeI TCCRAC 1008 1006 . . 1041 1044 + CviJI RGCY 1042 1042 . . 1043 1048 + Eco57MI CTGRAG 1064 1062 . . 1043 1048 + Eco57I CTGAAG 1064 1062 . . 1045 1049 + MboII GAAGA 1057 1056 . . 1048 1052 + MboII GAAGA 1060 1059 . . 1060 1065 + Cfr10I RCCGGY 1060 1064 . . 1060 1065 + BetI* WCCGGW 1060 1064 . . 1060 1065 + AgeI ACCGGT 1060 1064 . . 1061 1064 + HpaII CCGG 1061 1063 . . 1063 1067 + BmgT120I GGNCC 1064 1065 . . 1063 1067 + AvaII GGWCC 1063 1066 . . 1063 1067 + AsuI* GGNCC 1063 1066 . . 1068 1071 + TspEI AATT 1067 1071 . . 1079 1082 + TaiI ACGT 1082 1078 . . 1079 1082 + SetI ASST 1082 1078 . . 1079 1082 + MaeII ACGT 1079 1081 . . 1083 1087 + HphI GGTGA 1095 1094 . . 1078 1083 - BtrI CACGTC 1080 1080 . . 1089 1092 + CviJI RGCY 1090 1090 . . 1086 1090 - Hin4II* CCTTC 1080 1079 . . 1093 1098 + MmeI TCCRAC 1118 1116 . . 1093 1097 + Hpy188I TCNGA 1095 1094 . . 1108 1113 + BsgI GTGCAG 1129 1127 . . 1109 1112 + CviRI* TGCA 1110 1110 . . 1116 1119 + CviJI RGCY 1117 1117 . . 1113 1117 - Hin4II* CCTTC 1107 1106 . . 1144 1148 + Hpy188I TCNGA 1146 1145 . . 1149 1152 + TspEI AATT 1148 1152 . . 1153 1158 + EcoT22I ATGCAT 1157 1153 . . 1154 1157 + CviRI* TGCA 1155 1155 . . 1167 1171 + Hpy188I TCNGA 1169 1168 . . 1170 1173 + DpnI GATC 1171 1171 . . 1170 1173 + BstKTI GATC 1172 1170 . . 1170 1173 + MboI GATC 1169 1173 . . 1175 1178 + TaqI TCGA 1175 1177 . . 1187 1191 - BsmAI GTCTC 1181 1185 . . 1194 1197 + SetI ASST 1197 1193 . . 1196 1200 + MaeIII GTNAC 1195 1200 . . 1214 1219 + TatI WGTACW 1214 1218 . . 1215 1220 + Hpy166II GTNNAC 1217 1217 . . 1215 1218 + Csp6I GTAC 1215 1217 . . 1215 1218 + RsaI GTAC 1216 1216 . . 1231 1235 - BccI CCATC 1225 1226 . . 1241 1244 + TspEI AATT 1240 1244 . . 1259 1262 + MseI TTAA 1259 1261 . . 1276 1280 + MboII GAAGA 1288 1287 . . 1290 1293 + SetI ASST 1293 1289 . . 1288 1292 - Hin4II* CCTTC 1282 1281 . . 1294 1297 + MseI TTAA 1294 1296 . . 1298 1303 + SecI* CCNNGG 1298 1302 . . 1300 1303 - MnlI CCTC 1293 1292 . . 1306 1310 + TspDTI ATGAA 1321 1319 . . 1308 1321 + AjuI GAANNNNNNNTTGG 1300 1295 1332 1327 1308 1312 - Hin4II* CCTTC 1302 1301 . . 1313 1318 + HindIII AAGCTT 1313 1317 . . 1314 1317 + SetI ASST 1317 1313 . . 1314 1317 + CviJI RGCY 1315 1315 . . 1314 1317 + AluI AGCT 1315 1315 . . 1320 1324 + BmgT120I GGNCC 1321 1322 . . 1320 1324 + AvaII GGWCC 1320 1323 . . 1320 1324 + AsuI* GGNCC 1320 1323 . . 1322 1325 + SetI ASST 1325 1321 . . 1328 1333 + MfeI CAATTG 1328 1332 . . 1329 1332 + TspEI AATT 1328 1332 . . 1337 1340 + TaiI ACGT 1340 1336 . . 1337 1340 + SetI ASST 1340 1336 . . 1337 1340 + MaeII ACGT 1337 1339 . . 1341 1344 + AciI CCGC 1341 1343 . . 1345 1350 + ApoI RAATTY 1345 1349 . . 1346 1349 + TspEI AATT 1345 1349 . . 1351 1354 + FatI CATG 1350 1354 . . 1351 1354 + NlaIII CATG 1354 1350 . . 1351 1354 + CviAII CATG 1351 1353 . . 1354 1358 + BlsI GCNGC 1356 1355 . . 1354 1358 + BisI GCNGC 1355 1356 . . 1354 1358 + TseI GCWGC 1354 1357 . . 1354 1358 - BbvI GCAGC 1341 1345 . . 1360 1365 + MfeI CAATTG 1360 1364 . . 1361 1364 + TspEI AATT 1360 1364 . . 1374 1378 + HinfI GANTC 1374 1377 . . 1372 1376 - BsmAI GTCTC 1366 1370 . . 1377 1382 + Hpy178III* TCNNGA 1378 1380 . . 1374 1378 - PleI GAGTC 1368 1369 . . 1374 1378 - MlyI GAGTC 1368 1368 . . 1395 1398 + SetI ASST 1398 1394 . . 1395 1401 + SexAI ACCWGGT 1395 1400 . . 1396 1400 + BssKI CCNGG 1395 1400 . . 1396 1400 + BseBI CCWGG 1397 1398 . . 1396 1400 + ScrFI CCNGG 1397 1398 . . 1396 1400 + EcoRII CCWGG 1395 1400 . . 1411 1416 + Hpy178III* TCNNGA 1412 1414 . . 1415 1419 + TfiI GAWTC 1415 1418 . . 1415 1419 + HinfI GANTC 1415 1418 . . 1420 1423 + MnlI CCTC 1430 1429 . . 1452 1457 + SecI* CCNNGG 1452 1456 . . 1453 1457 + BssKI CCNGG 1452 1457 . . 1453 1457 + BseBI CCWGG 1454 1455 . . 1453 1457 + ScrFI CCNGG 1454 1455 . . 1453 1457 + EcoRII CCWGG 1452 1457 . . 1461 1467 + TspRI CASTGNN 1467 1458 . . 1461 1466 - BtsI GCAGTG 1460 1458 . . 1477 1482 + TatI WGTACW 1477 1481 . . 1477 1482 + ScaI AGTACT 1479 1479 . . 1478 1481 + Csp6I GTAC 1478 1480 . . 1478 1481 + RsaI GTAC 1479 1479 . . 1482 1485 + CviRI* TGCA 1483 1483 . . 1485 1490 + VspI ATTAAT 1486 1488 . . 1486 1489 + MseI TTAA 1486 1488 . . 1504 1514 + MwoI GCNNNNNNNGC 1510 1507 . . 1504 1514 + BstAPI GCANNNNNTGC 1510 1507 . . 1515 1525 + BsiYI* CCNNNNNNNGG 1521 1518 . . 1524 1534 - BsaXI ACNNNNNCTCC 1546 1543 1516 1513 1536 1539 + CviRI* TGCA 1537 1537 . . 1537 1541 + SfaNI GCATC 1546 1550 . . 1555 1559 + Hpy188I TCNGA 1557 1556 . . 1558 1562 - BccI CCATC 1552 1553 . . 1570 1574 - HphI GGTGA 1562 1561 . . 1603 1608 + HaeII RGCGCY 1607 1603 . . 1604 1607 + Hin6I GCGC 1604 1606 . . 1604 1607 + GlaI GCGC 1605 1605 . . 1604 1607 + HhaI GCGC 1606 1604 . . 1621 1624 + MaeI CTAG 1621 1623 . . 1623 1626 + SetI ASST 1626 1622 . . 1623 1626 + CviJI RGCY 1624 1624 . . 1623 1626 + AluI AGCT 1624 1624 . . 1624 1634 + MwoI GCNNNNNNNGC 1630 1627 . . 1630 1635 + NlaIV GGNNCC 1632 1632 . . 1630 1635 + HgiCI* GGYRCC 1630 1634 . . 1631 1636 + SduI GDGCHC 1635 1631 . . 1631 1636 + BseSI GKGCMC 1635 1631 . . 1638 1641 + CviJI RGCY 1639 1639 . . 1639 1649 + MwoI GCNNNNNNNGC 1645 1642 . . 1639 1643 - BceAI ACGGC 1624 1626 . . 1645 1655 + MwoI GCNNNNNNNGC 1651 1648 . . 1645 1649 + BlsI GCNGC 1647 1646 . . 1645 1649 + BisI GCNGC 1646 1647 . . 1645 1649 + TseI GCWGC 1645 1648 . . 1645 1649 - BbvI GCAGC 1632 1636 . . 1653 1656 + CviJI RGCY 1654 1654 . . 1660 1664 + BslFI GGGAC 1674 1678 . . 1666 1675 + XmnI GAANNNNTTC 1670 1670 . . 1688 1693 + XhoII RGATCY 1688 1692 . . 1688 1693 + BglII AGATCT 1688 1692 . . 1689 1699 + Hin4I GAYNNNNNVTC 1680 1675 1712 1707 1689 1692 + DpnI GATC 1690 1690 . . 1689 1692 + BstKTI GATC 1691 1689 . . 1689 1692 + MboI GATC 1688 1692 . . 1695 1700 + NlaIV GGNNCC 1697 1697 . . 1696 1700 + BmgT120I GGNCC 1697 1698 . . 1696 1700 + AvaII GGWCC 1696 1699 . . 1696 1700 + AsuI* GGNCC 1696 1699 . . 1689 1699 - Hin4I GAYNNNNNVTC 1712 1707 1680 1675 1699 1703 + BssKI CCNGG 1698 1703 . . 1699 1703 + BseBI CCWGG 1700 1701 . . 1699 1703 + ScrFI CCNGG 1700 1701 . . 1699 1703 + EcoRII CCWGG 1698 1703 . . 1701 1704 + SetI ASST 1704 1700 . . 1709 1712 + TspEI AATT 1708 1712 . . 1714 1718 + Tsp45I GTSAC 1713 1718 . . 1714 1718 + MaeIII GTNAC 1713 1718 . . 1720 1724 + TauI GCSGC 1723 1720 . . 1720 1724 + BlsI GCNGC 1722 1721 . . 1720 1724 + BisI GCNGC 1721 1722 . . 1721 1724 + AciI CCGC 1721 1723 . . 1748 1753 + SmlI CTYRAG 1748 1752 . . 1752 1755 + SetI ASST 1755 1751 . . 1752 1755 + CviJI RGCY 1753 1753 . . 1752 1755 + AluI AGCT 1753 1753 . . 1748 1753 - Bce83I* CTTGAG 1733 1731 . . 1764 1773 + MslI CAYNNNNRTG 1768 1768 . . 1773 1778 + Cac8I GCNNGC 1775 1775 . . 1775 1780 + PvuII CAGCTG 1777 1777 . . 1775 1780 + NspBII* CMGCKG 1777 1777 . . 1776 1779 + SetI ASST 1779 1775 . . 1776 1779 + CviJI RGCY 1777 1777 . . 1776 1779 + AluI AGCT 1777 1777 . . 1777 1782 + Cac8I GCNNGC 1779 1779 . . 1780 1783 + CviJI RGCY 1781 1781 . . 1780 1783 + HaeIII GGCC 1781 1781 . . 1780 1784 + BmgT120I GGNCC 1781 1782 . . 1780 1784 + AsuI* GGNCC 1780 1783 . . 1796 1801 - TsoI TARCCA 1786 1784 . . 1806 1817 + BcgI CGANNNNNNTGC 1795 1793 1829 1827 1808 1812 + TspDTI ATGAA 1823 1821 . . 1810 1814 + MboII GAAGA 1822 1821 . . 1813 1817 - SfaNI GCATC 1803 1807 . . 1833 1836 + CviRI* TGCA 1834 1834 . . 1840 1850 + Hin4I GAYNNNNNVTC 1831 1826 1863 1858 1840 1850 - Hin4I GAYNNNNNVTC 1863 1858 1831 1826 1851 1856 + Cac8I GCNNGC 1853 1853 . . 1854 1857 + SetI ASST 1857 1853 . . 1854 1857 + CviJI RGCY 1855 1855 . . 1854 1857 + AluI AGCT 1855 1855 . . 1862 1866 + Tsp4CI* ACNGT 1864 1863 . . 1870 1874 + HinfI GANTC 1870 1873 . . 1870 1874 - PleI GAGTC 1864 1865 . . 1870 1874 - MlyI GAGTC 1864 1864 . . 1877 1887 + FalI AAGNNNNNCTT 1868 1863 1900 1895 1877 1887 - FalI AAGNNNNNCTT 1900 1895 1868 1863 1894 1899 + Hpy166II GTNNAC 1896 1896 . . 1894 1899 + HpaI GTTAAC 1896 1896 . . 1894 1899 + HindII GTYRAC 1896 1896 . . 1895 1898 + MseI TTAA 1895 1897 . . 1899 1904 + SmlI CTYRAG 1899 1903 . . 1899 1904 + Bce83I* CTTGAG 1920 1918 . . 1913 1918 + ApoI RAATTY 1913 1917 . . 1914 1917 + TspEI AATT 1913 1917 . . 1917 1922 + Hpy178III* TCNNGA 1918 1920 . . 1923 1927 + TfiI GAWTC 1923 1926 . . 1923 1927 + HinfI GANTC 1923 1926 . . 1926 1931 + Hpy178III* TCNNGA 1927 1929 . . 1930 1934 - SfaNI GCATC 1920 1924 . . 1936 1941 + Hpy166II GTNNAC 1938 1938 . . 1946 1951 + HindIII AAGCTT 1946 1950 . . 1947 1950 + SetI ASST 1950 1946 . . 1947 1950 + CviJI RGCY 1948 1948 . . 1947 1950 + AluI AGCT 1948 1948 . . 1961 1966 + BsaAI YACGTR 1963 1963 . . 1961 1966 + PmaCI CACGTG 1963 1963 . . 1962 1965 + TaiI ACGT 1965 1961 . . 1962 1965 + SetI ASST 1965 1961 . . 1962 1965 + MaeII ACGT 1962 1964 . . 1962 1967 + AflIII ACRYGT 1962 1966 . . 1994 1998 + Tsp4CI* ACNGT 1996 1995 . . 1997 2000 + Csp6I GTAC 1997 1999 . . 1997 2000 + RsaI GTAC 1998 1998 . . 2000 2005 + McrI* CGRYCG 2003 2001 . . 2000 2005 + PvuI CGATCG 2003 2001 . . 2001 2004 + DpnI GATC 2002 2002 . . 2001 2004 + BstKTI GATC 2003 2001 . . 2001 2004 + MboI GATC 2000 2004 . . 2012 2016 + Tsp45I GTSAC 2011 2016 . . 2012 2016 + MaeIII GTNAC 2011 2016 . . 2015 2019 + Tsp4CI* ACNGT 2017 2016 . . 2018 2022 + MaeIII GTNAC 2017 2022 . . 2023 2027 + MboII GAAGA 2035 2034 . . 2024 2034 + FalI AAGNNNNNCTT 2015 2010 2047 2042 2028 2031 + CviJI RGCY 2029 2029 . . 2024 2034 - FalI AAGNNNNNCTT 2047 2042 2015 2010 2041 2046 + MmeI TCCRAC 2066 2064 . . 2052 2055 + CviRI* TGCA 2053 2053 . . 2067 2071 + TfiI GAWTC 2067 2070 . . 2067 2071 + HinfI GANTC 2067 2070 . . 2099 2102 + MseI TTAA 2099 2101 . . 2102 2105 + TspEI AATT 2101 2105 . . 2107 2112 + Hpy166II GTNNAC 2109 2109 . . 2107 2112 + HindII GTYRAC 2109 2109 . . 2115 2118 + CviRI* TGCA 2116 2116 . . 2119 2124 + NlaIV GGNNCC 2121 2121 . . 2120 2124 + BmgT120I GGNCC 2121 2122 . . 2120 2124 + AvaII GGWCC 2120 2123 . . 2120 2124 + AsuI* GGNCC 2120 2123 . . 2122 2126 - TspGWI ACGGA 2112 2110 . . 2132 2137 - TaqII CACCCA 2122 2120 . . 2145 2150 + Hpy178III* TCNNGA 2146 2148 . . 2149 2153 + TfiI GAWTC 2149 2152 . . 2149 2153 + HinfI GANTC 2149 2152 . . 2144 2149 - Eco57MI CTGRAG 2129 2127 . . 2144 2149 - GsuI CTGGAG 2129 2127 . . 2158 2162 + HphI GGTGA 2170 2169 . . 2159 2163 + Tsp45I GTSAC 2158 2163 . . 2159 2163 + MaeIII GTNAC 2158 2163 . . 2156 2159 - AciI CCGC 2156 2158 . . 2174 2177 + Csp6I GTAC 2174 2176 . . 2174 2177 + RsaI GTAC 2175 2175 . . 2178 2183 + TsoI TARCCA 2194 2192 . . 2191 2201 + BsiYI* CCNNNNNNNGG 2197 2194 . . 2191 2201 + PflMI CCANNNNNTGG 2197 2194 . . 2194 2197 + SetI ASST 2197 2193 . . 2201 2205 + MaeIII GTNAC 2200 2205 . . 2199 2204 - TsoI TARCCA 2189 2187 . . 2207 2210 + MaeI CTAG 2207 2209 . . 2213 2218 + TatI WGTACW 2213 2217 . . 2214 2217 + Csp6I GTAC 2214 2216 . . 2214 2217 + RsaI GTAC 2215 2215 . . 2216 2220 + Tsp4CI* ACNGT 2218 2217 . . 2217 2223 + TspRI CASTGNN 2223 2214 . . 2221 2226 + NlaIV GGNNCC 2223 2223 . . 2221 2226 + HgiCI* GGYRCC 2221 2225 . . 2224 2234 + MwoI GCNNNNNNNGC 2230 2227 . . 2229 2235 + TspRI CASTGNN 2235 2226 . . 2232 2235 + CviRI* TGCA 2233 2233 . . 2229 2234 - BtsI GCAGTG 2228 2226 . . 2236 2239 + SetI ASST 2239 2235 . . 2237 2241 + Hin4II* CCTTC 2247 2246 . . 2253 2259 + TspRI CASTGNN 2259 2250 . . 2253 2258 - BtsI GCAGTG 2252 2250 . . 2273 2278 + Eco57MI CTGRAG 2294 2292 . . 2273 2278 + Eco57I CTGAAG 2294 2292 . . 2277 2280 + SetI ASST 2280 2276 . . 2275 2279 - Hin4II* CCTTC 2269 2268 . . 2298 2301 + SetI ASST 2301 2297 . . 2298 2301 + CviJI RGCY 2299 2299 . . 2298 2301 + AluI AGCT 2299 2299 . . 2319 2323 - Hin4II* CCTTC 2313 2312 . . 2323 2326 - MnlI CCTC 2316 2315 . . 2328 2333 + XbaI TCTAGA 2328 2332 . . 2328 2333 + Hpy178III* TCNNGA 2329 2331 . . 2329 2332 + MaeI CTAG 2329 2331 . . 2333 2337 + Tsp4CI* ACNGT 2335 2334 . . 2336 2340 + Tsp45I GTSAC 2335 2340 . . 2336 2340 + MaeIII GTNAC 2335 2340 . . 2357 2361 + Hpy188I TCNGA 2359 2358 . . 2368 2373 + HindIII AAGCTT 2368 2372 . . 2369 2372 + SetI ASST 2372 2368 . . 2369 2372 + CviJI RGCY 2370 2370 . . 2369 2372 + AluI AGCT 2370 2370 . . 2379 2382 + DpnI GATC 2380 2380 . . 2379 2382 + BstKTI GATC 2381 2379 . . 2379 2382 + MboI GATC 2378 2382 . . 2379 2383 - BinI* GGATC 2373 2374 . . 2394 2398 - BsrI ACTGG 2394 2392 . . #--------------------------------------- # # # AatII: # [GACGTC] # 959 1441 # # AccI: # [GTMKAC] # 470 1930 # # AciI: # [CCGC] # 244 380 435 1341 # # AclI: # [AACGTT] # 677 1723 # # AcyI: # [GRCGYC] # 956 1444 # # AflIII: # [ACRYGT] # 438 1962 # # AgeI: # [ACCGGT] # 1060 1340 # # AjuI: # [GAANNNNNNNTTGG] # 32 1068 1300 # # AluI: # [AGCT] # 24 30 35 71 78 93 # 129 232 309 351 433 615 # # ApoI: # [RAATTY] # 174 178 366 487 568 627 # # AsuI*: # [GGNCC] # 84 257 280 340 376 1063 # # AsuII: # [TTCGAA] # 508 1892 # # AvaI: # [CYCGRG] # 96 2304 # # AvaII: # [GGWCC] # 257 280 376 424 1063 # # BalI: # [TGGCCA] # 12 314 608 1466 # # BbvI: # [GCAGC] # 291 459 768 882 # # BbvII*: # [GAAGAC] # 804 1596 # # BccI: # [CCATC] # 85 327 453 687 848 # # Bce83I*: # [CTTGAG] # 187 480 1733 # # BceAI: # [ACGGC] # 776 1624 # # BcgI: # [CGANNNNNNTGC] # 34 571 1795 # # BetI*: # [WCCGGW] # 1060 1340 # # BglII: # [AGATCT] # 712 1688 # # BinI*: # [GGATC] # 27 589 1784 # # BisI: # [GCNGC] # 75 291 459 679 896 # # BlsI: # [GCNGC] # 75 291 459 678 897 # # BmeT110I: # [CYCGRG] # 97 2303 # # BmgT120I: # [GGNCC] # 84 257 279 340 376 1064 # # BmtI: # [GCTAGC] # 242 2158 # # BsaAI: # [YACGTR] # 430 437 1533 # # BsaXI: # [ACNNNNNCTCC] # 30 30 688 798 854 # # BseBI: # [CCWGG] # 57 246 700 1397 # # BseGI: # [GGATG] # 96 555 1749 # # BseMII: # [CTCAG] # 94 757 1549 # # BseSI: # [GKGCMC] # 765 1635 # # BsgI: # [GTGCAG] # 1129 1271 # # BsiYI*: # [CCNNNNNNNGG] # 203 676 1521 # # BslFI: # [GGGAC] # 726 1674 # # BsmAI: # [GTCTC] # 185 490 691 1034 # # BspCNI: # [CTCAG] # 95 757 1548 # # BsrDI: # [GCAATG] # 36 197 376 1791 # # BsrI: # [ACTGG] # 6 123 800 1471 # # BssKI: # [CCNGG] # 57 246 702 1395 # # BstAPI: # [GCANNNNNTGC] # 890 1510 # # BstKTI: # [GATC] # 19 33 252 312 336 378 # 519 551 # # BstXI: # [CCANNNNNNTGG] # 393 537 1470 # # BtgZI: # [GCGATG] # 200 2200 # # BtrI: # [CACGTC] # 1080 1320 # # BtsI: # [GCAGTG] # 24 148 768 1460 # # Cac8I: # [GCNNGC] # 4 74 240 547 1535 # # Cfr10I: # [RCCGGY] # 1060 1340 # # CfrI: # [YGGCCR] # 12 314 606 1468 # # ClaI: # [ATCGAT] # 533 1867 # # Csp6I: # [GTAC] # 4 40 82 141 177 186 # 204 263 519 784 # # CspCI: # [CAANNNNNGTGG] # 35 447 1918 # # CviAII: # [CATG] # 25 41 298 363 624 1049 # # CviJI: # [RGCY] # 4 6 7 12 13 15 # 15 17 24 24 25 27 # 27 30 35 37 48 56 # 58 71 74 79 81 82 # 83 85 93 99 126 198 # 270 270 309 # # CviRI*: # [TGCA] # 45 54 63 99 117 167 # 194 219 297 328 817 # # DdeI: # [CTNAG] # 103 153 757 1387 # # DpnI: # [GATC] # 20 32 252 312 336 378 # 519 551 # # Eco31I: # [GGTCTC] # 691 1709 # # Eco57I: # [CTGAAG] # 58 106 137 869 1230 # # Eco57MI: # [CTGRAG] # 58 106 106 108 137 165 # 655 1065 # # EcoRI: # [GAATTC] # 718 1682 # # EcoRII: # [CCWGG] # 57 246 702 1395 # # EcoT22I: # [ATGCAT] # 1157 1243 # # EspI*: # [GCTNAGC] # 1013 1387 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 115 353 401 # 1435 # # FatI: # [CATG] # 25 40 298 363 624 1050 # # FokI: # [GGATG] # 103 555 1742 # # GlaI: # [GCGC] # 795 1605 # # GsuI: # [CTGGAG] # 106 271 655 1368 # # HaeII: # [RGCGCY] # 793 1607 # # HaeIII: # [GGCC] # 12 37 79 314 492 619 # 847 # # HgaI: # [GACGC] # 781 1619 # # HgiCI*: # [GGYRCC] # 179 591 1630 # # HhaI: # [GCGC] # 794 1606 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 22 32 # 32 32 32 119 537 634 # 960 # # Hin4II*: # [CCTTC] # 20 22 27 36 44 48 # 75 87 99 165 175 657 # 945 # # Hin6I: # [GCGC] # 796 1604 # # HindII: # [GTYRAC] # 67 213 291 404 1425 # # HindIII: # [AAGCTT] # 32 422 613 633 700 # # HinfI: # [GANTC] # 12 27 39 41 53 82 # 105 105 144 251 455 523 # 563 # # HpaI: # [GTTAAC] # 504 1896 # # HpaII: # [CCGG] # 1061 1339 # # HphI: # [GGTGA] # 77 126 168 230 291 433 # 467 608 # # Hpy166II: # [GTNNAC] # 42 67 171 291 404 679 # 746 # # Hpy178III*: # [TCNNGA] # 9 34 71 137 182 183 # 199 207 219 235 418 506 # # Hpy188I: # [TCNGA] # 15 23 41 51 109 326 # 388 645 802 # # Hpy99I: # [CGWCG] # 961 1439 # # Ksp632I*: # [CTCTTC] # 107 198 2095 # # MaeI: # [CTAG] # 27 52 71 76 122 176 # 224 239 586 827 # # MaeII: # [ACGT] # 62 123 248 258 279 367 # 438 625 # # MaeIII: # [GTNAC] # 6 42 65 135 141 205 # 298 518 990 # # MboI: # [GATC] # 22 30 252 312 336 378 # 519 551 # # MboII: # [GAAGA] # 3 9 25 27 44 64 # 107 124 175 195 213 228 # 287 365 534 # # McrI*: # [CGRYCG] # 397 2003 # # MfeI: # [CAATTG] # 32 277 1040 1051 # # MlyI: # [GAGTC] # 496 536 563 805 # # MmeI: # [TCCRAC] # 110 334 348 660 948 # # MnlI: # [CCTC] # 30 48 81 81 84 137 # 408 645 886 # # MseI: # [TTAA] # 22 35 192 204 264 301 # 366 409 607 # # MslI: # [CAYNNNNRTG] # 632 1768 # # MwoI: # [GCNNNNNNNGC] # 6 15 120 170 256 579 # 1254 # # NheI: # [GCTAGC] # 238 2162 # # NlaIII: # [CATG] # 25 44 298 363 624 1046 # # NlaIV: # [GGNNCC] # 65 102 177 424 1632 # # NspBII*: # [CMGCKG] # 623 1777 # # PflMI: # [CCANNNNNTGG] # 203 2197 # # PleI: # [GAGTC] # 496 536 563 805 # # PmaCI: # [CACGTG] # 437 1963 # # PspXI: # [VCTCGAGB] # 96 2304 # # PvuI: # [CGATCG] # 397 2003 # # PvuII: # [CAGCTG] # 623 1777 # # RsaI: # [GTAC] # 4 40 83 141 177 185 # 204 263 519 784 # # SalI: # [GTCGAC] # 469 1931 # # ScaI: # [AGTACT] # 921 1479 # # ScrFI: # [CCNGG] # 57 246 700 1397 # # SduI: # [GDGCHC] # 765 1635 # # SecI*: # [CCNNGG] # 154 948 1298 # # SetI: # [ASST] # 8 15 15 16 18 21 # 24 24 28 33 35 41 # 42 51 58 63 65 71 # 74 75 78 78 93 96 # 115 169 185 228 232 349 # # SexAI: # [ACCWGGT] # 1005 1395 # # SfaNI: # [GCATC] # 9 117 174 180 237 257 # 399 480 547 # # SfeI*: # [CTRYAG] # 248 518 1634 # # SmlI: # [CTYRAG] # 96 151 501 1652 # # SnaBI: # [TACGTA] # 430 1970 # # TaiI: # [ACGT] # 65 123 248 258 279 367 # 435 625 # # TaqI: # [TCGA] # 25 38 97 216 373 426 # 1225 # # TaqII: # [CACCCA] # 278 2122 # # TatI: # [WGTACW] # 187 263 736 1214 # # TauI: # [GCSGC] # 677 1723 # # TfiI: # [GAWTC] # 12 39 82 105 105 144 # 251 508 523 631 # # TseI: # [GCWGC] # 291 459 755 895 # # TsoI: # [TARCCA] # 5 206 256 403 1530 # # Tsp45I: # [GTSAC] # 65 147 177 298 1713 # # Tsp4CI*: # [ACNGT] # 9 20 21 65 117 120 # 132 189 201 299 1227 # # TspDTI: # [ATGAA] # 16 56 81 105 125 260 # 330 348 502 577 # # TspEI: # [AATT] # 15 16 17 17 18 23 # 57 81 88 92 99 142 # 155 188 205 207 299 333 # 348 # # TspGWI: # [ACGGA] # 230 288 784 1098 # # TspRI: # [CASTGNN] # 12 24 141 544 756 923 # # TstI: # [CACNNNNNNTCC] # 32 573 1795 # # Tth111I: # [GACNNNGTC] # 702 1698 # # VspI: # [ATTAAT] # 914 1486 # # XbaI: # [TCTAGA] # 72 2328 # # XcmI: # [CCANNNNNNNNNTGG] # 336 2064 # # XhoI: # [CTCGAG] # 96 2304 # # XhoII: # [RGATCY] # 712 1688 # # XmnI: # [GAANNNNTTC] # 27 116 730 1527 # # ZraI: # [GACGTC] # 957 1443 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2400 # Reported_sequences: 1 # Reported_hitcount: 567 #---------------------------------------