Restriction Fragments for YCL019W



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The currently selected gene/sequence is :

YCL019W on chromosome III from coordinates 85102 to 90415.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25944.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + TfiI GAWTC 4 7 . . 4 8 + HinfI GANTC 4 7 . . 14 17 + TspEI AATT 13 17 . . 30 33 + TaiI ACGT 33 29 . . 30 33 + SetI ASST 33 29 . . 30 33 + MaeII ACGT 30 32 . . 35 40 + Ksp632I* CTCTTC 41 44 . . 36 40 - MboII GAAGA 28 27 . . 40 43 + FatI CATG 39 43 . . 40 43 + NlaIII CATG 43 39 . . 40 43 + CviAII CATG 40 42 . . 38 42 - TspDTI ATGAA 28 26 . . 46 51 + HaeII RGCGCY 50 46 . . 47 50 + Hin6I GCGC 47 49 . . 47 50 + GlaI GCGC 48 48 . . 47 50 + HhaI GCGC 49 47 . . 61 65 + MaeIII GTNAC 60 65 . . 68 72 + DdeI CTNAG 68 71 . . 79 83 + BccI CCATC 87 88 . . 76 80 - BslFI GGGAC 61 65 . . 87 92 + Hpy178III* TCNNGA 88 90 . . 90 95 + XhoII RGATCY 90 94 . . 91 94 + DpnI GATC 92 92 . . 91 94 + BstKTI GATC 93 91 . . 91 94 + MboI GATC 90 94 . . 91 95 - BinI* GGATC 85 86 . . 93 97 - TspGWI ACGGA 83 81 . . 99 102 + CviJI RGCY 100 100 . . 100 110 + MwoI GCNNNNNNNGC 106 103 . . 100 104 - BceAI ACGGC 85 87 . . 108 111 + SetI ASST 111 107 . . 108 111 + CviJI RGCY 109 109 . . 108 111 + AluI AGCT 109 109 . . 115 118 + TspEI AATT 114 118 . . 120 125 + BetI* WCCGGW 120 124 . . 121 124 + HpaII CCGG 121 123 . . 124 129 + ApoI RAATTY 124 128 . . 125 128 + TspEI AATT 124 128 . . 136 140 + TfiI GAWTC 136 139 . . 136 140 + HinfI GANTC 136 139 . . 143 147 + DdeI CTNAG 143 146 . . 146 149 + SetI ASST 149 145 . . 149 152 + MseI TTAA 149 151 . . 151 154 + TspEI AATT 150 154 . . 163 167 - BsmAI GTCTC 157 161 . . 171 174 + SetI ASST 174 170 . . 172 176 + BssKI CCNGG 171 176 . . 172 176 + BseBI CCWGG 173 174 . . 172 177 + SecI* CCNNGG 172 176 . . 172 176 + ScrFI CCNGG 173 174 . . 172 176 + EcoRII CCWGG 171 176 . . 175 179 + BslFI GGGAC 189 193 . . 182 187 + PvuII CAGCTG 184 184 . . 182 187 + NspBII* CMGCKG 184 184 . . 183 186 + SetI ASST 186 182 . . 183 186 + CviJI RGCY 184 184 . . 183 186 + AluI AGCT 184 184 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 198 202 + BccI CCATC 206 207 . . 199 208 + MslI CAYNNNNRTG 203 203 . . 205 208 + FatI CATG 204 208 . . 205 208 + NlaIII CATG 208 204 . . 205 208 + CviAII CATG 205 207 . . 208 212 + BsmAI GTCTC 213 217 . . 214 217 + MnlI CCTC 224 223 . . 212 217 - BseRI GAGGAG 203 201 . . 219 222 + SetI ASST 222 218 . . 219 224 + BspMI ACCTGC 228 232 . . 224 229 - Eco57MI CTGRAG 209 207 . . 229 232 + Csp6I GTAC 229 231 . . 229 232 + RsaI GTAC 230 230 . . 224 229 - Eco57I CTGAAG 209 207 . . 234 237 + SetI ASST 237 233 . . 235 238 + MnlI CCTC 245 244 . . 238 248 + BsiYI* CCNNNNNNNGG 244 241 . . 238 248 + PflMI CCANNNNNTGG 244 241 . . 249 253 + Tsp4CI* ACNGT 251 250 . . 252 255 + Csp6I GTAC 252 254 . . 252 255 + RsaI GTAC 253 253 . . 263 267 + BceAI ACGGC 279 281 . . 267 270 + FatI CATG 266 270 . . 267 270 + NlaIII CATG 270 266 . . 267 270 + CviAII CATG 267 269 . . 285 288 + SetI ASST 288 284 . . 285 288 + CviJI RGCY 286 286 . . 285 288 + AluI AGCT 286 286 . . 291 294 + CviJI RGCY 292 292 . . 291 294 + HaeIII GGCC 292 292 . . 293 296 + MnlI CCTC 303 302 . . 293 303 + BsiYI* CCNNNNNNNGG 299 296 . . 299 303 + BsrI ACTGG 304 302 . . 299 304 + BfiI ACTGGG 309 308 . . 302 307 + SduI GDGCHC 306 302 . . 302 307 + BseSI GKGCMC 306 302 . . 318 322 + Tsp4CI* ACNGT 320 319 . . 331 334 + TaiI ACGT 334 330 . . 331 334 + SetI ASST 334 330 . . 331 334 + MaeII ACGT 331 333 . . 331 336 + AflIII ACRYGT 331 335 . . 335 340 + Hpy166II GTNNAC 337 337 . . 330 335 - BtrI CACGTC 332 332 . . 349 352 + TaiI ACGT 352 348 . . 349 352 + SetI ASST 352 348 . . 349 352 + MaeII ACGT 349 351 . . 351 355 + Tsp45I GTSAC 350 355 . . 351 355 + MaeIII GTNAC 350 355 . . 353 359 + AarI CACCTGC 363 367 . . 354 357 + SetI ASST 357 353 . . 354 359 + BspMI ACCTGC 363 367 . . 352 356 - HphI GGTGA 344 343 . . 369 374 + BetI* WCCGGW 369 373 . . 370 373 + HpaII CCGG 370 372 . . 372 377 + NlaIV GGNNCC 374 374 . . 372 376 + BmgT120I GGNCC 373 374 . . 372 376 + AvaII GGWCC 372 375 . . 372 376 + AsuI* GGNCC 372 375 . . 384 388 - TspGWI ACGGA 374 372 . . 392 403 + TstI CACNNNNNNTCC 383 378 415 410 393 397 + Tsp4CI* ACNGT 395 394 . . 407 413 + TspRI CASTGNN 413 404 . . 409 413 + DdeI CTNAG 409 412 . . 412 417 + TatI WGTACW 412 416 . . 412 417 + ScaI AGTACT 414 414 . . 413 416 + Csp6I GTAC 413 415 . . 409 413 - BspCNI CTCAG 401 399 . . 409 413 - BseMII CTCAG 400 398 . . 413 416 + RsaI GTAC 414 414 . . 419 422 + MnlI CCTC 429 428 . . 423 426 + SetI ASST 426 422 . . 421 425 - HphI GGTGA 413 412 . . 427 430 + DpnI GATC 428 428 . . 427 430 + BstKTI GATC 429 427 . . 427 430 + MboI GATC 426 430 . . 427 431 - BinI* GGATC 421 422 . . 433 438 + ClaI ATCGAT 434 436 . . 434 437 + TaqI TCGA 434 436 . . 436 440 + TfiI GAWTC 436 439 . . 436 440 + HinfI GANTC 436 439 . . 444 448 + TfiI GAWTC 444 447 . . 444 448 + HinfI GANTC 444 447 . . 454 458 + Hpy188I TCNGA 456 455 . . 455 460 + Eco57MI CTGRAG 476 474 . . 455 460 + Eco57I CTGAAG 476 474 . . 456 467 + BdaI TGANNNNNNTCA 445 443 479 477 460 463 + Csp6I GTAC 460 462 . . 460 463 + RsaI GTAC 461 461 . . 462 465 + SetI ASST 465 461 . . 463 466 + MnlI CCTC 473 472 . . 464 469 + SmlI CTYRAG 464 468 . . 456 467 - BdaI TGANNNNNNTCA 479 477 445 443 468 471 + SetI ASST 471 467 . . 468 471 + CviJI RGCY 469 469 . . 468 471 + AluI AGCT 469 469 . . 464 469 - Bce83I* CTTGAG 449 447 . . 470 474 + DdeI CTNAG 470 473 . . 470 474 - BspCNI CTCAG 462 460 . . 470 474 - BseMII CTCAG 461 459 . . 472 476 - BsmAI GTCTC 466 470 . . 479 482 + SetI ASST 482 478 . . 480 484 + HphI GGTGA 492 491 . . 491 494 + TaiI ACGT 494 490 . . 491 494 + SetI ASST 494 490 . . 491 494 + MaeII ACGT 491 493 . . 511 514 + MseI TTAA 511 513 . . 517 521 + Hpy188I TCNGA 519 518 . . 520 524 + MboII GAAGA 532 531 . . 536 539 + FatI CATG 535 539 . . 536 539 + NlaIII CATG 539 535 . . 536 539 + CviAII CATG 536 538 . . 542 545 + MseI TTAA 542 544 . . 544 549 + ApoI RAATTY 544 548 . . 545 548 + TspEI AATT 544 548 . . 554 558 + Hpy188I TCNGA 556 555 . . 564 568 + MboII GAAGA 576 575 . . 580 584 + HphI GGTGA 592 591 . . 581 585 + Tsp45I GTSAC 580 585 . . 581 585 + MaeIII GTNAC 580 585 . . 600 604 + BssKI CCNGG 599 604 . . 600 604 + BseBI CCWGG 601 602 . . 600 605 + SecI* CCNNGG 600 604 . . 600 604 + ScrFI CCNGG 601 602 . . 600 604 + EcoRII CCWGG 599 604 . . 604 608 + HphI GGTGA 616 615 . . 629 633 + TspDTI ATGAA 644 642 . . 631 635 + MboII GAAGA 643 642 . . 636 641 + NspI RCATGY 640 636 . . 637 640 + FatI CATG 636 640 . . 637 640 + NlaIII CATG 640 636 . . 637 640 + CviAII CATG 637 639 . . 655 658 + SetI ASST 658 654 . . 656 660 + Hin4II* CCTTC 666 665 . . 677 681 - TspDTI ATGAA 667 665 . . 692 695 + FatI CATG 691 695 . . 692 695 + NlaIII CATG 695 691 . . 692 695 + CviAII CATG 692 694 . . 704 709 + ApoI RAATTY 704 708 . . 705 708 + TspEI AATT 704 708 . . 714 717 + TspEI AATT 713 717 . . 715 720 + VspI ATTAAT 716 718 . . 716 719 + MseI TTAA 716 718 . . 718 721 + TspEI AATT 717 721 . . 729 733 - FokI GGATG 715 719 . . 729 733 - BseGI GGATG 728 726 . . 736 740 + Tsp4CI* ACNGT 738 737 . . 764 767 + CviRI* TGCA 765 765 . . 777 782 + Hpy178III* TCNNGA 778 780 . . 782 785 + TspEI AATT 781 785 . . 784 787 + MseI TTAA 784 786 . . 798 801 + SetI ASST 801 797 . . 798 801 + CviJI RGCY 799 799 . . 798 801 + AluI AGCT 799 799 . . 809 812 + SetI ASST 812 808 . . 811 816 + SmlI CTYRAG 811 815 . . 811 816 + Bce83I* CTTGAG 832 830 . . 816 819 + Csp6I GTAC 816 818 . . 816 819 + RsaI GTAC 817 817 . . 819 823 + Hpy99I CGWCG 823 818 . . 821 825 + TspGWI ACGGA 836 834 . . 826 831 + TatI WGTACW 826 830 . . 827 830 + Csp6I GTAC 827 829 . . 827 830 + RsaI GTAC 828 828 . . 833 838 - EcoP15I CAGCAG 805 807 . . 849 852 + TspEI AATT 848 852 . . 853 857 + Tsp4CI* ACNGT 855 854 . . 860 863 + Csp6I GTAC 860 862 . . 860 863 + RsaI GTAC 861 861 . . 864 867 + DpnI GATC 865 865 . . 864 867 + BstKTI GATC 866 864 . . 864 867 + MboI GATC 863 867 . . 870 874 + Hpy188I TCNGA 872 871 . . 875 878 + CviJI RGCY 876 876 . . 873 876 - MnlI CCTC 866 865 . . 912 915 + CviJI RGCY 913 913 . . 912 915 + HaeIII GGCC 913 913 . . 917 922 + Hpy166II GTNNAC 919 919 . . 917 922 + HindII GTYRAC 919 919 . . 929 932 + MseI TTAA 929 931 . . 933 936 + SetI ASST 936 932 . . 940 945 + BetI* WCCGGW 940 944 . . 941 944 + HpaII CCGG 941 943 . . 943 947 + HphI GGTGA 955 954 . . 944 948 + Tsp45I GTSAC 943 948 . . 944 948 + MaeIII GTNAC 943 948 . . 956 959 + SetI ASST 959 955 . . 959 964 + SnaBI TACGTA 961 961 . . 959 964 + BsaAI YACGTR 961 961 . . 960 963 + TaiI ACGT 963 959 . . 960 963 + SetI ASST 963 959 . . 960 963 + MaeII ACGT 960 962 . . 974 977 + Csp6I GTAC 974 976 . . 974 977 + RsaI GTAC 975 975 . . 987 990 + FatI CATG 986 990 . . 987 990 + NlaIII CATG 990 986 . . 987 990 + CviAII CATG 987 989 . . 988 992 + TspDTI ATGAA 1003 1001 . . 1001 1004 + TspEI AATT 1000 1004 . . 1013 1018 + ApoI RAATTY 1013 1017 . . 1014 1017 + TspEI AATT 1013 1017 . . 1021 1024 + MseI TTAA 1021 1023 . . 1043 1048 + Hpy178III* TCNNGA 1044 1046 . . 1043 1048 + BspHI TCATGA 1043 1047 . . 1044 1047 + FatI CATG 1043 1047 . . 1044 1047 + NlaIII CATG 1047 1043 . . 1044 1047 + CviAII CATG 1044 1046 . . 1045 1049 + TspDTI ATGAA 1060 1058 . . 1047 1051 + TfiI GAWTC 1047 1050 . . 1047 1051 + HinfI GANTC 1047 1050 . . 1059 1063 + Tsp4CI* ACNGT 1061 1060 . . 1062 1066 - BslFI GGGAC 1047 1051 . . 1130 1133 + SetI ASST 1133 1129 . . 1132 1136 + MaeIII GTNAC 1131 1136 . . 1144 1147 + TspEI AATT 1143 1147 . . 1149 1153 + Hpy188I TCNGA 1151 1150 . . 1177 1187 + MwoI GCNNNNNNNGC 1183 1180 . . 1177 1181 + BlsI GCNGC 1179 1178 . . 1177 1181 + BisI GCNGC 1178 1179 . . 1177 1181 + BbvI GCAGC 1189 1193 . . 1177 1181 + TseI GCWGC 1177 1180 . . 1185 1188 + SetI ASST 1188 1184 . . 1185 1188 + CviJI RGCY 1186 1186 . . 1185 1188 + AluI AGCT 1186 1186 . . 1192 1197 + SspI AATATT 1194 1194 . . 1205 1208 + MaeI CTAG 1205 1207 . . 1210 1215 + ApoI RAATTY 1210 1214 . . 1211 1214 + TspEI AATT 1210 1214 . . 1215 1220 + SmlI CTYRAG 1215 1219 . . 1215 1220 - Bce83I* CTTGAG 1200 1198 . . 1221 1225 + HphI GGTGA 1233 1232 . . 1222 1227 + Hpy166II GTNNAC 1224 1224 . . 1230 1235 + BclI TGATCA 1230 1234 . . 1231 1234 + DpnI GATC 1232 1232 . . 1231 1234 + BstKTI GATC 1233 1231 . . 1231 1234 + MboI GATC 1230 1234 . . 1234 1243 + MslI CAYNNNNRTG 1238 1238 . . 1237 1242 + VspI ATTAAT 1238 1240 . . 1238 1241 + MseI TTAA 1238 1240 . . 1241 1245 + TspDTI ATGAA 1256 1254 . . 1243 1247 + TfiI GAWTC 1243 1246 . . 1243 1247 + HinfI GANTC 1243 1246 . . 1249 1253 + Tsp4CI* ACNGT 1251 1250 . . 1254 1258 - TspDTI ATGAA 1244 1242 . . 1266 1271 + SmlI CTYRAG 1266 1270 . . 1266 1271 + AflII CTTAAG 1266 1270 . . 1267 1270 + MseI TTAA 1267 1269 . . 1271 1276 + BtgZI GCGATG 1286 1290 . . 1285 1289 + DdeI CTNAG 1285 1288 . . 1291 1295 + DdeI CTNAG 1291 1294 . . 1295 1298 + CviJI RGCY 1296 1296 . . 1295 1298 + HaeIII GGCC 1296 1296 . . 1296 1301 + Cac8I GCNNGC 1298 1298 . . 1310 1314 + TfiI GAWTC 1310 1313 . . 1310 1314 + HinfI GANTC 1310 1313 . . 1314 1318 + DdeI CTNAG 1314 1317 . . 1317 1320 + CviJI RGCY 1318 1318 . . 1334 1338 + HinfI GANTC 1334 1337 . . 1337 1340 + TaqI TCGA 1337 1339 . . 1334 1338 - PleI GAGTC 1328 1329 . . 1334 1338 - MlyI GAGTC 1328 1328 . . 1351 1354 + SetI ASST 1354 1350 . . 1354 1359 + BclI TGATCA 1354 1358 . . 1355 1358 + DpnI GATC 1356 1356 . . 1355 1358 + BstKTI GATC 1357 1355 . . 1355 1358 + MboI GATC 1354 1358 . . 1359 1362 + SetI ASST 1362 1358 . . 1360 1364 + Hin4II* CCTTC 1370 1369 . . 1357 1361 - HphI GGTGA 1349 1348 . . 1370 1374 + TfiI GAWTC 1370 1373 . . 1370 1374 + HinfI GANTC 1370 1373 . . 1373 1378 + Hpy178III* TCNNGA 1374 1376 . . 1378 1381 + SetI ASST 1381 1377 . . 1378 1381 + CviJI RGCY 1379 1379 . . 1378 1381 + AluI AGCT 1379 1379 . . 1395 1399 + Hpy188I TCNGA 1397 1396 . . 1398 1401 + DpnI GATC 1399 1399 . . 1398 1401 + BstKTI GATC 1400 1398 . . 1398 1401 + MboI GATC 1397 1401 . . 1402 1405 + CviJI RGCY 1403 1403 . . 1418 1421 + FatI CATG 1417 1421 . . 1418 1421 + NlaIII CATG 1421 1417 . . 1418 1421 + CviAII CATG 1418 1420 . . 1420 1423 + CviRI* TGCA 1421 1421 . . 1425 1430 + TaqII CACCCA 1441 1439 . . 1430 1433 + TspEI AATT 1429 1433 . . 1433 1437 + Hpy188I TCNGA 1435 1434 . . 1449 1452 + TaqI TCGA 1449 1451 . . 1451 1455 - SfaNI GCATC 1441 1445 . . 1492 1496 - MboII GAAGA 1484 1483 . . 1507 1511 + Hpy188I TCNGA 1509 1508 . . 1512 1516 + BceAI ACGGC 1528 1530 . . 1514 1519 + NlaIV GGNNCC 1516 1516 . . 1514 1519 + HgiCI* GGYRCC 1514 1518 . . 1555 1558 + CviJI RGCY 1556 1556 . . 1562 1565 + DpnI GATC 1563 1563 . . 1562 1565 + BstKTI GATC 1564 1562 . . 1562 1565 + MboI GATC 1561 1565 . . 1568 1571 + MseI TTAA 1568 1570 . . 1581 1584 + SetI ASST 1584 1580 . . 1581 1584 + CviJI RGCY 1582 1582 . . 1581 1584 + AluI AGCT 1582 1582 . . 1582 1587 + Cac8I GCNNGC 1584 1584 . . 1585 1588 + CviJI RGCY 1586 1586 . . 1584 1589 - TsoI TARCCA 1574 1572 . . 1595 1600 + SspI AATATT 1597 1597 . . 1604 1609 + Cac8I GCNNGC 1606 1606 . . 1632 1635 + DpnI GATC 1633 1633 . . 1632 1635 + BstKTI GATC 1634 1632 . . 1632 1635 + MboI GATC 1631 1635 . . 1634 1638 + Hpy188I TCNGA 1636 1635 . . 1636 1640 + FokI GGATG 1649 1653 . . 1636 1640 + BseGI GGATG 1642 1640 . . 1641 1644 + Csp6I GTAC 1641 1643 . . 1637 1641 - BccI CCATC 1631 1632 . . 1641 1644 + RsaI GTAC 1642 1642 . . 1643 1647 + Tsp4CI* ACNGT 1645 1644 . . 1649 1652 + MaeI CTAG 1649 1651 . . 1651 1654 + SetI ASST 1654 1650 . . 1651 1654 + CviJI RGCY 1652 1652 . . 1651 1654 + AluI AGCT 1652 1652 . . 1667 1670 + FatI CATG 1666 1670 . . 1667 1670 + NlaIII CATG 1670 1666 . . 1667 1670 + CviAII CATG 1667 1669 . . 1671 1675 - BsmAI GTCTC 1665 1669 . . 1680 1684 + BsrI ACTGG 1685 1683 . . 1682 1687 - TsoI TARCCA 1672 1670 . . 1698 1701 + SetI ASST 1701 1697 . . 1702 1705 + TspEI AATT 1701 1705 . . 1706 1710 + Hin4II* CCTTC 1716 1715 . . 1722 1725 + SetI ASST 1725 1721 . . 1722 1725 + CviJI RGCY 1723 1723 . . 1722 1725 + AluI AGCT 1723 1723 . . 1737 1740 + TaiI ACGT 1740 1736 . . 1737 1740 + SetI ASST 1740 1736 . . 1737 1740 + MaeII ACGT 1737 1739 . . 1739 1744 + Hpy166II GTNNAC 1741 1741 . . 1739 1744 + HindII GTYRAC 1741 1741 . . 1774 1778 - TspGWI ACGGA 1764 1762 . . 1778 1781 + MseI TTAA 1778 1780 . . 1786 1789 + TaqI TCGA 1786 1788 . . 1788 1793 + BsmI GAATGC 1794 1792 . . 1798 1803 + NspI RCATGY 1802 1798 . . 1799 1802 + FatI CATG 1798 1802 . . 1799 1802 + NlaIII CATG 1802 1798 . . 1799 1802 + CviAII CATG 1799 1801 . . 1809 1813 + Hpy188I TCNGA 1811 1810 . . 1819 1823 + Hpy188I TCNGA 1821 1820 . . 1825 1829 + BsmAI GTCTC 1830 1834 . . 1829 1834 + SmlI CTYRAG 1829 1833 . . 1829 1834 + AflII CTTAAG 1829 1833 . . 1830 1833 + MseI TTAA 1830 1832 . . 1834 1838 + MboII GAAGA 1846 1845 . . 1837 1842 + BsmI GAATGC 1843 1841 . . 1840 1843 + CviRI* TGCA 1841 1841 . . 1844 1848 + MaeIII GTNAC 1843 1848 . . 1859 1863 + TfiI GAWTC 1859 1862 . . 1855 1859 - Hin4II* CCTTC 1849 1848 . . 1859 1863 + HinfI GANTC 1859 1862 . . 1862 1866 + Hpy188I TCNGA 1864 1863 . . 1883 1888 + Cac8I GCNNGC 1885 1885 . . 1883 1888 + BmtI GCTAGC 1887 1883 . . 1883 1888 + NheI GCTAGC 1883 1887 . . 1884 1887 + MaeI CTAG 1884 1886 . . 1890 1899 + MslI CAYNNNNRTG 1894 1894 . . 1900 1905 + Hpy178III* TCNNGA 1901 1903 . . 1905 1909 + Tsp4CI* ACNGT 1907 1906 . . 1931 1940 + MslI CAYNNNNRTG 1935 1935 . . 1936 1941 + BspLU11I* ACATGT 1936 1940 . . 1936 1941 + NspI RCATGY 1940 1936 . . 1936 1941 + AflIII ACRYGT 1936 1940 . . 1937 1940 + FatI CATG 1936 1940 . . 1937 1940 + NlaIII CATG 1940 1936 . . 1937 1940 + CviAII CATG 1937 1939 . . 1946 1950 + BinI* GGATC 1954 1955 . . 1947 1950 + DpnI GATC 1948 1948 . . 1947 1950 + BstKTI GATC 1949 1947 . . 1947 1950 + MboI GATC 1946 1950 . . 1949 1954 + Hpy178III* TCNNGA 1950 1952 . . 1954 1964 + BaeI ACNNNNGTAYC 1943 1938 1976 1971 1960 1963 + Csp6I GTAC 1960 1962 . . 1960 1963 + RsaI GTAC 1961 1961 . . 1967 1971 + TfiI GAWTC 1967 1970 . . 1967 1971 + HinfI GANTC 1967 1970 . . 1971 1976 + NdeI CATATG 1972 1974 . . 1975 1986 + BdaI TGANNNNNNTCA 1964 1962 1998 1996 1977 1980 + CviJI RGCY 1978 1978 . . 1986 1991 + TatI WGTACW 1986 1990 . . 1975 1986 - BdaI TGANNNNNNTCA 1998 1996 1964 1962 1986 1991 + ScaI AGTACT 1988 1988 . . 1987 1990 + Csp6I GTAC 1987 1989 . . 1987 1990 + RsaI GTAC 1988 1988 . . 1992 1995 + CviRI* TGCA 1993 1993 . . 2009 2013 + BmgT120I GGNCC 2010 2011 . . 2009 2013 + AvaII GGWCC 2009 2012 . . 2009 2013 + AsuI* GGNCC 2009 2012 . . 2014 2019 + TatI WGTACW 2014 2018 . . 2014 2019 + Bsp1407I TGTACA 2014 2018 . . 2015 2018 + Csp6I GTAC 2015 2017 . . 2015 2018 + RsaI GTAC 2016 2016 . . 2032 2042 + FalI AAGNNNNNCTT 2023 2018 2055 2050 2034 2039 + Hpy166II GTNNAC 2036 2036 . . 2034 2039 + SduI GDGCHC 2038 2034 . . 2034 2039 + BseSI GKGCMC 2038 2034 . . 2034 2039 + ApaLI GTGCAC 2034 2038 . . 2034 2039 + HgiAI* GWGCWC 2038 2034 . . 2035 2038 + CviRI* TGCA 2036 2036 . . 2038 2041 + SetI ASST 2041 2037 . . 2039 2043 + Hin4II* CCTTC 2049 2048 . . 2032 2042 - FalI AAGNNNNNCTT 2055 2050 2023 2018 2056 2061 + Hpy166II GTNNAC 2058 2058 . . 2076 2080 + TfiI GAWTC 2076 2079 . . 2076 2080 + HinfI GANTC 2076 2079 . . 2087 2092 + Hpy166II GTNNAC 2089 2089 . . 2084 2089 - TaqII CACCCA 2074 2072 . . 2089 2092 + Csp6I GTAC 2089 2091 . . 2089 2092 + RsaI GTAC 2090 2090 . . 2101 2106 + McrI* CGRYCG 2104 2102 . . 2103 2107 + Tsp4CI* ACNGT 2105 2104 . . 2105 2109 + Hpy99I CGWCG 2109 2104 . . 2107 2112 + Hpy178III* TCNNGA 2108 2110 . . 2111 2115 + MboII GAAGA 2123 2122 . . 2114 2118 + TfiI GAWTC 2114 2117 . . 2114 2118 + HinfI GANTC 2114 2117 . . 2122 2125 + MnlI CCTC 2132 2131 . . 2137 2142 + ClaI ATCGAT 2138 2140 . . 2138 2141 + TaqI TCGA 2138 2140 . . 2154 2157 + MseI TTAA 2154 2156 . . 2158 2167 + XmnI GAANNNNTTC 2162 2162 . . 2163 2166 + TspEI AATT 2162 2166 . . 2171 2176 + Cac8I GCNNGC 2173 2173 . . 2174 2177 + FnuDII* CGCG 2175 2175 . . 2180 2183 + MaeI CTAG 2180 2182 . . 2187 2192 + Hpy178III* TCNNGA 2188 2190 . . 2188 2202 + XcmI CCANNNNNNNNNTGG 2195 2194 . . 2194 2198 + BinI* GGATC 2202 2203 . . 2195 2205 + Hin4I GAYNNNNNVTC 2186 2181 2218 2213 2195 2198 + DpnI GATC 2196 2196 . . 2195 2198 + BstKTI GATC 2197 2195 . . 2195 2198 + MboI GATC 2194 2198 . . 2191 2195 - BccI CCATC 2185 2186 . . 2201 2204 + CviJI RGCY 2202 2202 . . 2201 2206 + NlaIV GGNNCC 2203 2203 . . 2204 2208 + Hpy188I TCNGA 2206 2205 . . 2208 2213 + TatI WGTACW 2208 2212 . . 2209 2214 + Hpy166II GTNNAC 2211 2211 . . 2209 2212 + Csp6I GTAC 2209 2211 . . 2209 2212 + RsaI GTAC 2210 2210 . . 2223 2228 + Ksp632I* CTCTTC 2229 2232 . . 2224 2228 - MboII GAAGA 2216 2215 . . 2226 2230 - TspDTI ATGAA 2216 2214 . . 2248 2251 + SetI ASST 2251 2247 . . 2247 2250 - MnlI CCTC 2240 2239 . . 2257 2260 + CviRI* TGCA 2258 2258 . . 2258 2263 + NspI RCATGY 2262 2258 . . 2258 2263 + Cac8I GCNNGC 2260 2260 . . 2258 2263 + SphI GCATGC 2262 2258 . . 2259 2262 + FatI CATG 2258 2262 . . 2259 2262 + NlaIII CATG 2262 2258 . . 2259 2262 + CviAII CATG 2259 2261 . . 2271 2276 + DsaI* CCRYGG 2271 2275 . . 2271 2276 + SecI* CCNNGG 2271 2275 . . 2273 2277 + BceAI ACGGC 2289 2291 . . 2279 2283 + TfiI GAWTC 2279 2282 . . 2279 2283 + HinfI GANTC 2279 2282 . . 2282 2287 + XbaI TCTAGA 2282 2286 . . 2282 2287 + Hpy178III* TCNNGA 2283 2285 . . 2283 2286 + MaeI CTAG 2283 2285 . . 2286 2291 + SduI GDGCHC 2290 2286 . . 2286 2291 + HgiAI* GWGCWC 2290 2286 . . 2289 2297 + DraIII CACNNNGTG 2294 2291 . . 2292 2296 + Tsp4CI* ACNGT 2294 2293 . . 2309 2312 + MseI TTAA 2309 2311 . . 2316 2319 + Csp6I GTAC 2316 2318 . . 2316 2319 + RsaI GTAC 2317 2317 . . 2324 2327 + MseI TTAA 2324 2326 . . 2340 2346 + TspRI CASTGNN 2346 2337 . . 2340 2345 - BtsI GCAGTG 2339 2337 . . 2348 2357 + MslI CAYNNNNRTG 2352 2352 . . 2346 2350 - TspDTI ATGAA 2336 2334 . . 2351 2354 + CviRI* TGCA 2352 2352 . . 2352 2357 + BtsI GCAGTG 2359 2357 . . 2353 2359 + TspRI CASTGNN 2359 2350 . . 2348 2353 - BsrDI GCAATG 2347 2345 . . 2358 2363 + Hpy166II GTNNAC 2360 2360 . . 2358 2363 + AccI GTMKAC 2359 2361 . . 2363 2377 + XcmI CCANNNNNNNNNTGG 2370 2369 . . 2380 2384 + DdeI CTNAG 2380 2383 . . 2380 2384 + BspCNI CTCAG 2393 2391 . . 2380 2384 + BseMII CTCAG 2394 2392 . . 2382 2387 + EcoP15I CAGCAG 2412 2414 . . 2388 2391 + TaqI TCGA 2388 2390 . . 2390 2395 + ApoI RAATTY 2390 2394 . . 2391 2394 + TspEI AATT 2390 2394 . . 2406 2410 + Hpy188I TCNGA 2408 2407 . . 2410 2415 + ApoI RAATTY 2410 2414 . . 2411 2414 + TspEI AATT 2410 2414 . . 2413 2417 - TspDTI ATGAA 2403 2401 . . 2420 2424 + BsmAI GTCTC 2425 2429 . . 2423 2427 - HphI GGTGA 2415 2414 . . 2442 2445 + AciI CCGC 2442 2444 . . 2452 2457 + NspI RCATGY 2456 2452 . . 2453 2456 + FatI CATG 2452 2456 . . 2453 2456 + NlaIII CATG 2456 2452 . . 2453 2456 + CviAII CATG 2453 2455 . . 2455 2458 + CviRI* TGCA 2456 2456 . . 2456 2466 + MwoI GCNNNNNNNGC 2462 2459 . . 2458 2461 + SetI ASST 2461 2457 . . 2456 2461 - BspMI ACCTGC 2447 2451 . . 2464 2467 + SetI ASST 2467 2463 . . 2464 2467 + CviJI RGCY 2465 2465 . . 2464 2467 + AluI AGCT 2465 2465 . . 2470 2474 + BsrI ACTGG 2475 2473 . . 2491 2494 + SetI ASST 2494 2490 . . 2499 2504 + Hpy166II GTNNAC 2501 2501 . . 2499 2504 + HindII GTYRAC 2501 2501 . . 2496 2501 - TaqII GACCGA 2486 2484 . . 2503 2508 + Cfr10I RCCGGY 2503 2507 . . 2503 2508 + BetI* WCCGGW 2503 2507 . . 2503 2508 + AgeI ACCGGT 2503 2507 . . 2504 2507 + HpaII CCGG 2504 2506 . . 2513 2518 + Hpy166II GTNNAC 2515 2515 . . 2513 2518 + HpaI GTTAAC 2515 2515 . . 2513 2518 + HindII GTYRAC 2515 2515 . . 2514 2517 + MseI TTAA 2514 2516 . . 2527 2532 + Hpy178III* TCNNGA 2528 2530 . . 2531 2535 + HinfI GANTC 2531 2534 . . 2534 2537 + TaqI TCGA 2534 2536 . . 2531 2535 - PleI GAGTC 2525 2526 . . 2531 2535 - MlyI GAGTC 2525 2525 . . 2543 2552 + MslI CAYNNNNRTG 2547 2547 . . 2546 2549 + MnlI CCTC 2556 2555 . . 2543 2547 - FokI GGATG 2529 2533 . . 2543 2547 - BseGI GGATG 2542 2540 . . 2547 2552 - BsiI* CACGAG 2547 2551 . . 2558 2562 + BssKI CCNGG 2557 2562 . . 2558 2562 + BseBI CCWGG 2559 2560 . . 2553 2558 - BsmI GAATGC 2553 2551 . . 2558 2562 + ScrFI CCNGG 2559 2560 . . 2558 2562 + EcoRII CCWGG 2557 2562 . . 2560 2563 + SetI ASST 2563 2559 . . 2562 2566 + MaeIII GTNAC 2561 2566 . . 2573 2577 - FokI GGATG 2559 2563 . . 2573 2577 - BseGI GGATG 2572 2570 . . 2578 2582 + Tsp45I GTSAC 2577 2582 . . 2578 2582 + MaeIII GTNAC 2577 2582 . . 2575 2579 - TspGWI ACGGA 2565 2563 . . 2579 2584 + Hpy178III* TCNNGA 2580 2582 . . 2594 2597 + CviJI RGCY 2595 2595 . . 2593 2598 - TsoI TARCCA 2583 2581 . . 2608 2612 - MboII GAAGA 2600 2599 . . 2612 2616 + BccI CCATC 2620 2621 . . 2618 2621 + MseI TTAA 2618 2620 . . 2627 2631 + Tsp4CI* ACNGT 2629 2628 . . 2642 2645 + TspEI AATT 2641 2645 . . 2646 2649 + TaiI ACGT 2649 2645 . . 2646 2649 + SetI ASST 2649 2645 . . 2646 2649 + MaeII ACGT 2646 2648 . . 2660 2670 + Hin4I GAYNNNNNVTC 2651 2646 2683 2678 2660 2670 - Hin4I GAYNNNNNVTC 2683 2678 2651 2646 2673 2676 + TspEI AATT 2672 2676 . . 2677 2681 + BmgT120I GGNCC 2678 2679 . . 2677 2681 + AvaII GGWCC 2677 2680 . . 2677 2681 + AsuI* GGNCC 2677 2680 . . 2682 2685 + TspEI AATT 2681 2685 . . 2687 2690 + TspEI AATT 2686 2690 . . 2702 2705 + SetI ASST 2705 2701 . . 2700 2704 - HphI GGTGA 2692 2691 . . 2708 2717 + BsaBI GATNNNNATC 2712 2712 . . 2714 2717 + DpnI GATC 2715 2715 . . 2714 2717 + BstKTI GATC 2716 2714 . . 2714 2723 + BsaBI GATNNNNATC 2718 2718 . . 2714 2717 + MboI GATC 2713 2717 . . 2726 2729 + MseI TTAA 2726 2728 . . 2731 2734 + CviJI RGCY 2732 2732 . . 2737 2742 + TsoI TARCCA 2753 2751 . . 2760 2764 + TspDTI ATGAA 2775 2773 . . 2765 2769 + TspGWI ACGGA 2780 2778 . . 2775 2780 + NdeI CATATG 2776 2778 . . 2779 2784 + BclI TGATCA 2779 2783 . . 2780 2783 + DpnI GATC 2781 2781 . . 2780 2783 + BstKTI GATC 2782 2780 . . 2780 2783 + MboI GATC 2779 2783 . . 2790 2798 + AlwNI CAGNNNCTG 2795 2792 . . 2792 2796 + TfiI GAWTC 2792 2795 . . 2792 2796 + HinfI GANTC 2792 2795 . . 2795 2799 + Hpy188I TCNGA 2797 2796 . . 2797 2802 + BclI TGATCA 2797 2801 . . 2798 2801 + DpnI GATC 2799 2799 . . 2798 2801 + BstKTI GATC 2800 2798 . . 2798 2801 + MboI GATC 2797 2801 . . 2800 2805 + Hpy178III* TCNNGA 2801 2803 . . 2800 2805 + BspHI TCATGA 2800 2804 . . 2801 2804 + FatI CATG 2800 2804 . . 2801 2804 + NlaIII CATG 2804 2800 . . 2801 2804 + CviAII CATG 2801 2803 . . 2804 2814 + Hin4I GAYNNNNNVTC 2795 2790 2827 2822 2813 2817 + Hpy188I TCNGA 2815 2814 . . 2804 2814 - Hin4I GAYNNNNNVTC 2827 2822 2795 2790 2831 2835 + Hpy188I TCNGA 2833 2832 . . 2834 2837 + DpnI GATC 2835 2835 . . 2834 2837 + BstKTI GATC 2836 2834 . . 2834 2837 + MboI GATC 2833 2837 . . 2837 2840 + MnlI CCTC 2847 2846 . . 2834 2838 - BinI* GGATC 2828 2829 . . 2840 2843 + MaeI CTAG 2840 2842 . . 2843 2848 + Hpy166II GTNNAC 2845 2845 . . 2845 2856 + PpiI GAACNNNNNCTC 2837 2832 2869 2864 2845 2854 + XmnI GAANNNNTTC 2849 2849 . . 2849 2859 + Hin4I GAYNNNNNVTC 2840 2835 2872 2867 2849 2859 + Eam1105I GACNNNNNGTC 2854 2853 . . 2849 2859 - Hin4I GAYNNNNNVTC 2872 2867 2840 2835 2857 2861 - BslFI GGGAC 2842 2846 . . 2880 2883 + TspEI AATT 2879 2883 . . 2890 2895 + NlaIV GGNNCC 2892 2892 . . 2894 2898 - BsrI ACTGG 2894 2892 . . 2906 2909 + Csp6I GTAC 2906 2908 . . 2906 2909 + RsaI GTAC 2907 2907 . . 2907 2912 + BsaAI YACGTR 2909 2909 . . 2908 2911 + TaiI ACGT 2911 2907 . . 2908 2911 + SetI ASST 2911 2907 . . 2908 2911 + MaeII ACGT 2908 2910 . . 2910 2915 + Hpy166II GTNNAC 2912 2912 . . 2910 2915 + SduI GDGCHC 2914 2910 . . 2910 2915 + BseSI GKGCMC 2914 2910 . . 2910 2915 + ApaLI GTGCAC 2910 2914 . . 2910 2915 + HgiAI* GWGCWC 2914 2910 . . 2911 2914 + CviRI* TGCA 2912 2912 . . 2927 2931 - SfaNI GCATC 2917 2921 . . 2939 2943 + Hpy188I TCNGA 2941 2940 . . 2948 2953 + SspI AATATT 2950 2950 . . 2953 2957 - MboII GAAGA 2945 2944 . . 2957 2961 + BccI CCATC 2965 2966 . . 2966 2969 + Csp6I GTAC 2966 2968 . . 2966 2969 + RsaI GTAC 2967 2967 . . 2970 2973 + DpnI GATC 2971 2971 . . 2970 2973 + BstKTI GATC 2972 2970 . . 2970 2973 + MboI GATC 2969 2973 . . 2976 2981 + Hpy166II GTNNAC 2978 2978 . . 2976 2979 + Csp6I GTAC 2976 2978 . . 2976 2979 + RsaI GTAC 2977 2977 . . 2990 2995 + VspI ATTAAT 2991 2993 . . 2991 2994 + MseI TTAA 2991 2993 . . 3003 3006 + Csp6I GTAC 3003 3005 . . 3003 3006 + RsaI GTAC 3004 3004 . . 3012 3017 - TaqII CACCCA 3002 3000 . . 3017 3022 + KpnI GGTACC 3021 3017 . . 3017 3022 + NlaIV GGNNCC 3019 3019 . . 3017 3022 + HgiCI* GGYRCC 3017 3021 . . 3017 3022 + Acc65I GGTACC 3017 3021 . . 3018 3021 + Csp6I GTAC 3018 3020 . . 3018 3021 + RsaI GTAC 3019 3019 . . 3020 3024 + Tsp4CI* ACNGT 3022 3021 . . 3026 3030 + TfiI GAWTC 3026 3029 . . 3026 3030 + HinfI GANTC 3026 3029 . . 3029 3033 + Hpy188I TCNGA 3031 3030 . . 3043 3046 + SetI ASST 3046 3042 . . 3045 3048 + MaeI CTAG 3045 3047 . . 3041 3045 - HphI GGTGA 3033 3032 . . 3048 3057 + PshAI GACNNNNGTC 3052 3052 . . 3055 3060 + Hpy166II GTNNAC 3057 3057 . . 3055 3060 + AccI GTMKAC 3056 3058 . . 3059 3062 + SetI ASST 3062 3058 . . 3060 3064 + Hin4II* CCTTC 3070 3069 . . 3064 3070 + TspRI CASTGNN 3070 3061 . . 3067 3070 + CviRI* TGCA 3068 3068 . . 3064 3069 - BtsI GCAGTG 3063 3061 . . 3066 3071 - BsgI GTGCAG 3051 3049 . . 3076 3090 + XcmI CCANNNNNNNNNTGG 3083 3082 . . 3084 3095 + DrdI GACNNNNNNGTC 3090 3088 . . 3085 3088 + SetI ASST 3088 3084 . . 3085 3091 + SexAI ACCWGGT 3085 3090 . . 3086 3090 + BssKI CCNGG 3085 3090 . . 3086 3090 + BseBI CCWGG 3087 3088 . . 3086 3090 + ScrFI CCNGG 3087 3088 . . 3086 3090 + EcoRII CCWGG 3085 3090 . . 3093 3097 - BslFI GGGAC 3078 3082 . . 3110 3120 + Hin4I GAYNNNNNVTC 3101 3096 3133 3128 3116 3119 + SetI ASST 3119 3115 . . 3117 3120 + MnlI CCTC 3127 3126 . . 3132 3135 + MseI TTAA 3132 3134 . . 3134 3137 + TspEI AATT 3133 3137 . . 3161 3166 + AflIII ACRYGT 3161 3165 . . 3163 3166 + TaiI ACGT 3166 3162 . . 3163 3166 + SetI ASST 3166 3162 . . 3163 3166 + MaeII ACGT 3163 3165 . . 3168 3173 - TaqII CACCCA 3158 3156 . . 3174 3177 + Csp6I GTAC 3174 3176 . . 3174 3177 + RsaI GTAC 3175 3175 . . 3176 3180 + TspGWI ACGGA 3191 3189 . . 3181 3186 + NlaIV GGNNCC 3183 3183 . . 3179 3182 - MnlI CCTC 3172 3171 . . 3237 3240 + Csp6I GTAC 3237 3239 . . 3237 3240 + RsaI GTAC 3238 3238 . . 3243 3246 + MnlI CCTC 3253 3252 . . 3254 3264 + Hin4I GAYNNNNNVTC 3245 3240 3277 3272 3254 3264 + Eam1105I GACNNNNNGTC 3259 3258 . . 3255 3259 + Tsp4CI* ACNGT 3257 3256 . . 3263 3268 + MmeI TCCRAC 3288 3286 . . 3254 3264 - Hin4I GAYNNNNNVTC 3277 3272 3245 3240 3267 3271 + Tsp4CI* ACNGT 3269 3268 . . 3268 3274 + TspRI CASTGNN 3274 3265 . . 3272 3276 + TfiI GAWTC 3272 3275 . . 3272 3276 + HinfI GANTC 3272 3275 . . 3285 3289 + BccI CCATC 3293 3294 . . 3307 3310 + CviJI RGCY 3308 3308 . . 3327 3330 + MnlI CCTC 3337 3336 . . 3335 3344 + BsaBI GATNNNNATC 3339 3339 . . 3338 3342 + TspGWI ACGGA 3353 3351 . . 3340 3345 + XhoII RGATCY 3340 3344 . . 3340 3345 + NlaIV GGNNCC 3342 3342 . . 3340 3345 + BamHI GGATCC 3340 3344 . . 3340 3344 + BinI* GGATC 3348 3349 . . 3341 3344 + DpnI GATC 3342 3342 . . 3341 3344 + BstKTI GATC 3343 3341 . . 3341 3344 + MboI GATC 3340 3344 . . 3344 3347 + AciI CCGC 3344 3346 . . 3341 3345 - BinI* GGATC 3335 3336 . . 3343 3348 - EciI GGCGGA 3333 3331 . . 3354 3359 + ClaI ATCGAT 3355 3357 . . 3355 3358 + TaqI TCGA 3355 3357 . . 3357 3360 + DpnI GATC 3358 3358 . . 3357 3360 + BstKTI GATC 3359 3357 . . 3357 3360 + MboI GATC 3356 3360 . . 3362 3366 + Hpy188I TCNGA 3364 3363 . . 3359 3363 - MboII GAAGA 3351 3350 . . 3368 3371 + FatI CATG 3367 3371 . . 3368 3371 + NlaIII CATG 3371 3367 . . 3368 3371 + CviAII CATG 3368 3370 . . 3373 3377 + HinfI GANTC 3373 3376 . . 3373 3377 - PleI GAGTC 3367 3368 . . 3373 3377 - MlyI GAGTC 3367 3367 . . 3392 3402 + Hin4I GAYNNNNNVTC 3383 3378 3415 3410 3399 3402 + MnlI CCTC 3409 3408 . . 3410 3414 + MaeIII GTNAC 3409 3414 . . 3413 3417 + BceAI ACGGC 3429 3431 . . 3415 3418 + CviJI RGCY 3416 3416 . . 3419 3423 + TfiI GAWTC 3419 3422 . . 3419 3423 + HinfI GANTC 3419 3422 . . 3427 3432 + Hpy178III* TCNNGA 3428 3430 . . 3435 3438 + SetI ASST 3438 3434 . . 3436 3439 + MnlI CCTC 3446 3445 . . 3445 3450 + Hpy178III* TCNNGA 3446 3448 . . 3452 3455 + MseI TTAA 3452 3454 . . 3457 3461 + BccI CCATC 3465 3466 . . 3470 3474 + TspGWI ACGGA 3485 3483 . . 3479 3483 + Hpy188I TCNGA 3481 3480 . . 3517 3521 + Hpy188I TCNGA 3519 3518 . . 3524 3527 + TspEI AATT 3523 3527 . . 3528 3532 - BsrI ACTGG 3528 3526 . . 3534 3539 - TaqII CACCCA 3524 3522 . . 3544 3548 + FokI GGATG 3557 3561 . . 3544 3548 + BseGI GGATG 3550 3548 . . 3548 3552 + TfiI GAWTC 3548 3551 . . 3548 3552 + HinfI GANTC 3548 3551 . . 3559 3563 + Hpy188I TCNGA 3561 3560 . . 3585 3588 + DpnI GATC 3586 3586 . . 3585 3588 + BstKTI GATC 3587 3585 . . 3585 3588 + MboI GATC 3584 3588 . . 3593 3597 + MboII GAAGA 3605 3604 . . 3600 3604 + TspDTI ATGAA 3615 3613 . . 3610 3613 + TspEI AATT 3609 3613 . . 3615 3618 + SetI ASST 3618 3614 . . 3614 3617 - MnlI CCTC 3607 3606 . . 3617 3622 + BciVI GTATCC 3628 3627 . . 3620 3625 + Hpy178III* TCNNGA 3621 3623 . . 3621 3626 + BmeT110I CYCGRG 3622 3624 . . 3621 3626 + AvaI CYCGRG 3621 3625 . . 3624 3628 - BsmAI GTCTC 3618 3622 . . 3630 3633 + FatI CATG 3629 3633 . . 3630 3633 + NlaIII CATG 3633 3629 . . 3630 3633 + CviAII CATG 3630 3632 . . 3655 3660 + Hpy178III* TCNNGA 3656 3658 . . 3658 3663 + NlaIV GGNNCC 3660 3660 . . 3669 3672 + DpnI GATC 3670 3670 . . 3669 3672 + BstKTI GATC 3671 3669 . . 3669 3672 + MboI GATC 3668 3672 . . 3671 3674 + TaqI TCGA 3671 3673 . . 3673 3677 + MboII GAAGA 3685 3684 . . 3685 3690 + ApoI RAATTY 3685 3689 . . 3686 3689 + TspEI AATT 3685 3689 . . 3689 3692 + MseI TTAA 3689 3691 . . 3691 3694 + TspEI AATT 3690 3694 . . 3694 3697 + CviRI* TGCA 3695 3695 . . 3695 3699 + BlsI GCNGC 3697 3696 . . 3695 3699 + BisI GCNGC 3696 3697 . . 3695 3699 + BbvI GCAGC 3707 3711 . . 3695 3699 + TseI GCWGC 3695 3698 . . 3711 3722 + BdaI TGANNNNNNTCA 3700 3698 3734 3732 3715 3720 + ClaI ATCGAT 3716 3718 . . 3716 3719 + TaqI TCGA 3716 3718 . . 3718 3721 + DpnI GATC 3719 3719 . . 3718 3721 + BstKTI GATC 3720 3718 . . 3718 3721 + MboI GATC 3717 3721 . . 3711 3722 - BdaI TGANNNNNNTCA 3734 3732 3700 3698 3725 3729 - BsrI ACTGG 3725 3723 . . 3729 3734 + AsuII TTCGAA 3730 3732 . . 3730 3733 + TaqI TCGA 3730 3732 . . 3737 3740 + SetI ASST 3740 3736 . . 3739 3744 + SmlI CTYRAG 3739 3743 . . 3739 3744 + AflII CTTAAG 3739 3743 . . 3740 3743 + MseI TTAA 3740 3742 . . 3750 3754 + TspDTI ATGAA 3765 3763 . . 3757 3760 + TspEI AATT 3756 3760 . . 3763 3768 + PsiI TTATAA 3765 3765 . . 3799 3810 + BdaI TGANNNNNNTCA 3788 3786 3822 3820 3801 3806 + HindIII AAGCTT 3801 3805 . . 3802 3805 + SetI ASST 3805 3801 . . 3802 3805 + CviJI RGCY 3803 3803 . . 3802 3805 + AluI AGCT 3803 3803 . . 3799 3810 - BdaI TGANNNNNNTCA 3822 3820 3788 3786 3817 3820 + TspEI AATT 3816 3820 . . 3820 3825 + TsoI TARCCA 3836 3834 . . 3821 3824 + CviJI RGCY 3822 3822 . . 3836 3840 + TspDTI ATGAA 3851 3849 . . 3847 3852 - BciVI GTATCC 3841 3840 . . 3858 3863 + SspI AATATT 3860 3860 . . 3880 3885 + XhoII RGATCY 3880 3884 . . 3881 3884 + DpnI GATC 3882 3882 . . 3881 3884 + BstKTI GATC 3883 3881 . . 3881 3884 + MboI GATC 3880 3884 . . 3881 3885 - BinI* GGATC 3875 3876 . . 3915 3918 + MseI TTAA 3915 3917 . . 3925 3928 + TaiI ACGT 3928 3924 . . 3925 3928 + SetI ASST 3928 3924 . . 3925 3928 + MaeII ACGT 3925 3927 . . 3933 3938 + Hpy166II GTNNAC 3935 3935 . . 3933 3936 + Csp6I GTAC 3933 3935 . . 3929 3933 - BccI CCATC 3923 3924 . . 3933 3936 + RsaI GTAC 3934 3934 . . 3943 3946 + SetI ASST 3946 3942 . . 3943 3946 + CviJI RGCY 3944 3944 . . 3943 3946 + AluI AGCT 3944 3944 . . 3945 3948 + MaeI CTAG 3945 3947 . . 3955 3958 + CviRI* TGCA 3956 3956 . . 3960 3963 - MnlI CCTC 3953 3952 . . 3984 3989 + NdeI CATATG 3985 3987 . . 3989 3993 + TfiI GAWTC 3989 3992 . . 3989 3993 + HinfI GANTC 3989 3992 . . 3992 3996 + Hpy188I TCNGA 3994 3993 . . 3995 4005 + Hin4I GAYNNNNNVTC 3986 3981 4018 4013 3999 4002 + CviRI* TGCA 4000 4000 . . 3995 4005 - Hin4I GAYNNNNNVTC 4018 4013 3986 3981 4010 4014 + Tsp4CI* ACNGT 4012 4011 . . 4013 4016 + Csp6I GTAC 4013 4015 . . 4013 4016 + RsaI GTAC 4014 4014 . . 4016 4025 + MslI CAYNNNNRTG 4020 4020 . . 4024 4027 + CviRI* TGCA 4025 4025 . . 4026 4032 + TspRI CASTGNN 4032 4023 . . 4033 4038 + ZraI GACGTC 4035 4035 . . 4033 4038 + AcyI GRCGYC 4034 4036 . . 4033 4038 + AatII GACGTC 4037 4033 . . 4033 4044 + DrdI GACNNNNNNGTC 4039 4037 . . 4034 4037 + TaiI ACGT 4037 4033 . . 4034 4037 + SetI ASST 4037 4033 . . 4034 4037 + MaeII ACGT 4034 4036 . . 4036 4040 + Tsp45I GTSAC 4035 4040 . . 4036 4040 + MaeIII GTNAC 4035 4040 . . 4038 4044 + TspRI CASTGNN 4044 4035 . . 4039 4043 + Tsp4CI* ACNGT 4041 4040 . . 4076 4081 + PvuII CAGCTG 4078 4078 . . 4076 4081 + NspBII* CMGCKG 4078 4078 . . 4077 4080 + SetI ASST 4080 4076 . . 4077 4080 + CviJI RGCY 4078 4078 . . 4077 4080 + AluI AGCT 4078 4078 . . 4082 4092 + Hin4I GAYNNNNNVTC 4073 4068 4105 4100 4089 4092 + MnlI CCTC 4099 4098 . . 4109 4114 + EcoRV GATATC 4111 4111 . . 4118 4122 + MboII GAAGA 4130 4129 . . 4122 4125 + TspEI AATT 4121 4125 . . 4135 4138 + SetI ASST 4138 4134 . . 4136 4139 + MnlI CCTC 4146 4145 . . 4142 4152 + BsiYI* CCNNNNNNNGG 4148 4145 . . 4150 4153 + SetI ASST 4153 4149 . . 4164 4167 + TspEI AATT 4163 4167 . . 4171 4174 + TaiI ACGT 4174 4170 . . 4171 4174 + SetI ASST 4174 4170 . . 4171 4174 + MaeII ACGT 4171 4173 . . 4210 4213 + CviRI* TGCA 4211 4211 . . 4215 4219 + BsrI ACTGG 4220 4218 . . 4221 4225 + TspDTI ATGAA 4236 4234 . . 4230 4233 + MseI TTAA 4230 4232 . . 4241 4244 + MseI TTAA 4241 4243 . . 4247 4250 + TspEI AATT 4246 4250 . . 4257 4262 + NspI RCATGY 4261 4257 . . 4258 4261 + FatI CATG 4257 4261 . . 4258 4261 + NlaIII CATG 4261 4257 . . 4258 4261 + CviAII CATG 4258 4260 . . 4260 4263 + CviRI* TGCA 4261 4261 . . 4271 4274 + FnuDII* CGCG 4272 4272 . . 4261 4272 - BcgI CGANNNNNNTGC 4284 4282 4250 4248 4274 4278 + FokI GGATG 4287 4291 . . 4274 4278 + BseGI GGATG 4280 4278 . . 4272 4275 - AciI CCGC 4272 4274 . . 4275 4279 - BccI CCATC 4269 4270 . . 4281 4284 + FatI CATG 4280 4284 . . 4281 4284 + NlaIII CATG 4284 4280 . . 4281 4284 + CviAII CATG 4281 4283 . . 4290 4293 + MseI TTAA 4290 4292 . . 4304 4308 + MaeIII GTNAC 4303 4308 . . 4309 4312 + TspEI AATT 4308 4312 . . 4332 4343 + BdaI TGANNNNNNTCA 4321 4319 4355 4353 4332 4343 - BdaI TGANNNNNNTCA 4355 4353 4321 4319 4352 4355 + MseI TTAA 4352 4354 . . 4357 4360 + CviRI* TGCA 4358 4358 . . 4382 4387 + SmlI CTYRAG 4382 4386 . . 4383 4388 + Hpy178III* TCNNGA 4384 4386 . . 4382 4387 - Bce83I* CTTGAG 4367 4365 . . 4405 4415 + Hin4I GAYNNNNNVTC 4396 4391 4428 4423 4405 4415 - Hin4I GAYNNNNNVTC 4428 4423 4396 4391 4418 4422 + HphI GGTGA 4430 4429 . . 4416 4421 - TaqII CACCCA 4406 4404 . . 4434 4439 + ApoI RAATTY 4434 4438 . . 4435 4438 + TspEI AATT 4434 4438 . . 4441 4444 + Csp6I GTAC 4441 4443 . . 4441 4444 + RsaI GTAC 4442 4442 . . 4455 4465 + Hin4I GAYNNNNNVTC 4446 4441 4478 4473 4462 4472 + Hin4I GAYNNNNNVTC 4453 4448 4485 4480 4462 4465 + DpnI GATC 4463 4463 . . 4462 4465 + BstKTI GATC 4464 4462 . . 4462 4465 + MboI GATC 4461 4465 . . 4455 4465 - Hin4I GAYNNNNNVTC 4478 4473 4446 4441 4462 4472 - Hin4I GAYNNNNNVTC 4485 4480 4453 4448 4482 4487 + TatI WGTACW 4482 4486 . . 4483 4486 + Csp6I GTAC 4483 4485 . . 4483 4486 + RsaI GTAC 4484 4484 . . 4486 4489 + FatI CATG 4485 4489 . . 4486 4489 + NlaIII CATG 4489 4485 . . 4486 4489 + CviAII CATG 4486 4488 . . 4487 4491 + TspDTI ATGAA 4502 4500 . . 4491 4494 + TspEI AATT 4490 4494 . . 4495 4498 + SetI ASST 4498 4494 . . 4521 4524 + TspEI AATT 4520 4524 . . 4536 4539 + TaiI ACGT 4539 4535 . . 4536 4539 + SetI ASST 4539 4535 . . 4536 4539 + MaeII ACGT 4536 4538 . . 4538 4541 + Csp6I GTAC 4538 4540 . . 4538 4541 + RsaI GTAC 4539 4539 . . 4540 4543 + SetI ASST 4543 4539 . . 4565 4569 + DdeI CTNAG 4565 4568 . . 4569 4574 + SacI GAGCTC 4573 4569 . . 4569 4574 + Ecl136II GAGCTC 4571 4571 . . 4569 4574 + SduI GDGCHC 4573 4569 . . 4569 4574 + HgiJII* GRGCYC 4573 4569 . . 4569 4574 + HgiAI* GWGCWC 4573 4569 . . 4570 4573 + SetI ASST 4573 4569 . . 4570 4573 + CviJI RGCY 4571 4571 . . 4570 4573 + AluI AGCT 4571 4571 . . 4574 4578 + BssKI CCNGG 4573 4578 . . 4574 4578 + BseBI CCWGG 4575 4576 . . 4574 4578 + ScrFI CCNGG 4575 4576 . . 4574 4578 + EcoRII CCWGG 4573 4578 . . 4576 4579 + SetI ASST 4579 4575 . . 4572 4577 - Eco57MI CTGRAG 4557 4555 . . 4572 4577 - GsuI CTGGAG 4557 4555 . . 4578 4583 + Hpy166II GTNNAC 4580 4580 . . 4578 4583 + HindII GTYRAC 4580 4580 . . 4582 4588 + SexAI ACCWGGT 4582 4587 . . 4583 4587 + BssKI CCNGG 4582 4587 . . 4583 4587 + BseBI CCWGG 4584 4585 . . 4583 4587 + ScrFI CCNGG 4584 4585 . . 4583 4587 + EcoRII CCWGG 4582 4587 . . 4585 4588 + SetI ASST 4588 4584 . . 4600 4604 + BssKI CCNGG 4599 4604 . . 4600 4604 + BseBI CCWGG 4601 4602 . . 4600 4604 + ScrFI CCNGG 4601 4602 . . 4600 4604 + EcoRII CCWGG 4599 4604 . . 4603 4607 + FokI GGATG 4616 4620 . . 4603 4607 + BseGI GGATG 4609 4607 . . 4605 4609 + TspDTI ATGAA 4620 4618 . . 4610 4613 + MaeI CTAG 4610 4612 . . 4620 4624 + TspDTI ATGAA 4635 4633 . . 4622 4626 + MboII GAAGA 4634 4633 . . 4626 4630 + TspDTI ATGAA 4641 4639 . . 4642 4647 + TatI WGTACW 4642 4646 . . 4643 4646 + Csp6I GTAC 4643 4645 . . 4643 4646 + RsaI GTAC 4644 4644 . . 4646 4649 + FatI CATG 4645 4649 . . 4646 4649 + NlaIII CATG 4649 4645 . . 4646 4649 + CviAII CATG 4646 4648 . . 4647 4651 + TspDTI ATGAA 4662 4660 . . 4653 4656 + CviRI* TGCA 4654 4654 . . 4670 4673 + MaeI CTAG 4670 4672 . . 4672 4675 + SetI ASST 4675 4671 . . 4672 4675 + CviJI RGCY 4673 4673 . . 4672 4675 + AluI AGCT 4673 4673 . . 4677 4682 + NdeI CATATG 4678 4680 . . 4675 4679 - TspDTI ATGAA 4665 4663 . . 4682 4687 - MmeI TCCRAC 4663 4661 . . 4691 4696 + ApoI RAATTY 4691 4695 . . 4692 4695 + TspEI AATT 4691 4695 . . 4749 4752 + MnlI CCTC 4759 4758 . . 4752 4755 + MaeI CTAG 4752 4754 . . 4768 4771 + FatI CATG 4767 4771 . . 4768 4771 + NlaIII CATG 4771 4767 . . 4768 4771 + CviAII CATG 4768 4770 . . 4773 4778 + NdeI CATATG 4774 4776 . . 4781 4784 + MseI TTAA 4781 4783 . . 4787 4798 + CspCI CAANNNNNGTGG 4775 4773 4810 4808 4788 4791 + TspEI AATT 4787 4791 . . 4792 4795 + FatI CATG 4791 4795 . . 4792 4795 + NlaIII CATG 4795 4791 . . 4792 4795 + CviAII CATG 4792 4794 . . 4790 4794 - TspDTI ATGAA 4780 4778 . . 4797 4801 + BslFI GGGAC 4811 4815 . . 4803 4806 + MaeI CTAG 4803 4805 . . 4815 4818 + TspEI AATT 4814 4818 . . 4816 4821 + VspI ATTAAT 4817 4819 . . 4817 4820 + MseI TTAA 4817 4819 . . 4837 4840 + SetI ASST 4840 4836 . . 4845 4848 + CviJI RGCY 4846 4846 . . 4858 4863 + SpeI ACTAGT 4858 4862 . . 4859 4862 + MaeI CTAG 4859 4861 . . 4870 4880 + FalI AAGNNNNNCTT 4861 4856 4893 4888 4872 4877 + BtgZI GCGATG 4887 4891 . . 4874 4878 - SfaNI GCATC 4864 4868 . . 4870 4880 - FalI AAGNNNNNCTT 4893 4888 4861 4856 4881 4886 + NdeI CATATG 4882 4884 . . 4879 4883 - TspDTI ATGAA 4869 4867 . . 4886 4892 + BstEII GGTNACC 4886 4891 . . 4887 4891 + MaeIII GTNAC 4886 4891 . . 4888 4893 + TsoI TARCCA 4904 4902 . . 4906 4910 + Tsp45I GTSAC 4905 4910 . . 4906 4910 + MaeIII GTNAC 4905 4910 . . 4912 4915 + TspEI AATT 4911 4915 . . 4917 4921 + MaeIII GTNAC 4916 4921 . . 4935 4939 + TspGWI ACGGA 4950 4948 . . 4950 4953 - MnlI CCTC 4943 4942 . . 4957 4962 + SalI GTCGAC 4957 4961 . . 4957 4962 + Hpy166II GTNNAC 4959 4959 . . 4957 4962 + HindII GTYRAC 4959 4959 . . 4957 4962 + AccI GTMKAC 4958 4960 . . 4958 4961 + TaqI TCGA 4958 4960 . . 4966 4969 + CviJI RGCY 4967 4967 . . 4972 4977 + Hpy166II GTNNAC 4974 4974 . . 4972 4977 + HpaI GTTAAC 4974 4974 . . 4972 4977 + HindII GTYRAC 4974 4974 . . 4973 4976 + MseI TTAA 4973 4975 . . 4976 4981 + NspI RCATGY 4980 4976 . . 4977 4980 + FatI CATG 4976 4980 . . 4977 4980 + NlaIII CATG 4980 4976 . . 4977 4980 + CviAII CATG 4977 4979 . . 4979 4982 + CviRI* TGCA 4980 4980 . . 4989 4994 + SfeI* CTRYAG 4989 4993 . . 4994 5006 + BarI GAAGNNNNNNTAC 4986 4981 5018 5013 5014 5020 + TspRI CASTGNN 5020 5011 . . 5018 5027 + XmnI GAANNNNTTC 5022 5022 . . 5020 5023 + SetI ASST 5023 5019 . . 5020 5023 + CviJI RGCY 5021 5021 . . 5020 5023 + AluI AGCT 5021 5021 . . 5027 5030 + AciI CCGC 5027 5029 . . 5034 5045 + BdaI TGANNNNNNTCA 5023 5021 5057 5055 5040 5043 + SetI ASST 5043 5039 . . 5041 5044 + MnlI CCTC 5051 5050 . . 5042 5046 + DdeI CTNAG 5042 5045 . . 5042 5046 + BspCNI CTCAG 5055 5053 . . 5042 5046 + BseMII CTCAG 5056 5054 . . 5034 5045 - BdaI TGANNNNNNTCA 5057 5055 5023 5021 5046 5050 + Tsp45I GTSAC 5045 5050 . . 5046 5050 + MaeIII GTNAC 5045 5050 . . 5048 5056 + DraIII CACNNNGTG 5053 5050 . . 5049 5052 + SetI ASST 5052 5048 . . 5047 5051 - HphI GGTGA 5039 5038 . . 5055 5058 + CviRI* TGCA 5056 5056 . . 5064 5067 + MseI TTAA 5064 5066 . . 5077 5080 + TspEI AATT 5076 5080 . . 5082 5085 + MseI TTAA 5082 5084 . . 5085 5095 + FalI AAGNNNNNCTT 5076 5071 5108 5103 5087 5090 + CviJI RGCY 5088 5088 . . 5085 5095 - FalI AAGNNNNNCTT 5108 5103 5076 5071 5099 5109 + Hin4I GAYNNNNNVTC 5090 5085 5122 5117 5106 5116 + Hin4I GAYNNNNNVTC 5097 5092 5129 5124 5106 5109 + DpnI GATC 5107 5107 . . 5106 5109 + BstKTI GATC 5108 5106 . . 5106 5109 + MboI GATC 5105 5109 . . 5099 5109 - Hin4I GAYNNNNNVTC 5122 5117 5090 5085 5113 5116 + DpnI GATC 5114 5114 . . 5113 5116 + BstKTI GATC 5115 5113 . . 5113 5116 + MboI GATC 5112 5116 . . 5106 5116 - Hin4I GAYNNNNNVTC 5129 5124 5097 5092 5122 5125 + TspEI AATT 5121 5125 . . 5124 5127 + MseI TTAA 5124 5126 . . 5128 5133 + Hpy166II GTNNAC 5130 5130 . . 5128 5133 + AccI GTMKAC 5129 5131 . . 5136 5140 + TspDTI ATGAA 5151 5149 . . 5138 5142 + MboII GAAGA 5150 5149 . . 5138 5143 - Ksp632I* CTCTTC 5133 5136 . . 5144 5149 + ApoI RAATTY 5144 5148 . . 5145 5148 + TspEI AATT 5144 5148 . . 5174 5179 + BsrDI GCAATG 5181 5179 . . 5183 5187 + DdeI CTNAG 5183 5186 . . 5179 5183 - BsmAI GTCTC 5173 5177 . . 5189 5199 + Hin4I GAYNNNNNVTC 5180 5175 5212 5207 5190 5194 + TspDTI ATGAA 5205 5203 . . 5189 5199 - Hin4I GAYNNNNNVTC 5212 5207 5180 5175 5200 5203 + SetI ASST 5203 5199 . . 5207 5210 + TspEI AATT 5206 5210 . . 5213 5218 + SnaBI TACGTA 5215 5215 . . 5213 5218 + BsaAI YACGTR 5215 5215 . . 5214 5217 + TaiI ACGT 5217 5213 . . 5214 5217 + SetI ASST 5217 5213 . . 5214 5217 + MaeII ACGT 5214 5216 . . 5216 5221 + Hpy166II GTNNAC 5218 5218 . . 5216 5221 + BssNAI GTATAC 5218 5218 . . 5216 5221 + AccI GTMKAC 5217 5219 . . 5226 5229 + TaqI TCGA 5226 5228 . . 5226 5231 + Hpy178III* TCNNGA 5227 5229 . . 5228 5232 - BsmAI GTCTC 5222 5226 . . 5228 5233 - Eco31I GGTCTC 5222 5226 . . 5236 5240 + MboII GAAGA 5248 5247 . . 5242 5247 - BsrDI GCAATG 5241 5239 . . 5257 5267 + Hin4I GAYNNNNNVTC 5248 5243 5280 5275 5263 5266 + SetI ASST 5266 5262 . . 5264 5267 + MnlI CCTC 5274 5273 . . 5265 5270 + Ksp632I* CTCTTC 5271 5274 . . 5269 5273 + Hpy188I TCNGA 5271 5270 . . 5266 5270 - MboII GAAGA 5258 5257 . . 5282 5287 + AhaIII* TTTAAA 5284 5284 . . 5283 5286 + MseI TTAA 5283 5285 . . 5291 5294 + MseI TTAA 5291 5293 . . 5305 5309 + TfiI GAWTC 5305 5308 . . 5305 5309 + HinfI GANTC 5305 5308 . . 5307 5311 - TspDTI ATGAA 5297 5295 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 363 4951 # # AatII: # [GACGTC] # 1277 4037 # # Acc65I: # [GGTACC] # 2297 3017 # # AccI: # [GTMKAC] # 88 97 171 697 1902 2359 # # AciI: # [CCGC] # 287 755 902 928 2442 # # AcyI: # [GRCGYC] # 1280 4034 # # AflII: # [CTTAAG] # 563 1266 1575 1910 # # AflIII: # [ACRYGT] # 331 1225 1605 2153 # # AgeI: # [ACCGGT] # 2503 2811 # # AhaIII*: # [TTTAAA] # 30 5284 # # AluI: # [AGCT] # 70 71 75 102 102 109 # 134 141 183 193 203 293 # 330 348 387 493 742 1338 # # AlwNI: # [CAGNNNCTG] # 2519 2795 # # ApaLI: # [GTGCAC] # 876 2034 2404 # # ApoI: # [RAATTY] # 20 124 160 170 197 257 # 309 420 453 749 1180 1275 # # AsuI*: # [GGNCC] # 372 668 1637 2637 # # AsuII: # [TTCGAA] # 1584 3730 # # AvaI: # [CYCGRG] # 1693 3621 # # AvaII: # [GGWCC] # 372 668 1637 2637 # # BaeI: # [ACNNNNGTAYC] # 33 1943 3338 # # BamHI: # [GGATCC] # 1974 3340 # # BarI: # [GAAGNNNNNNTAC] # 32 296 4986 # # BbvI: # [GCAGC] # 1189 1607 2518 # # BccI: # [CCATC] # 87 119 172 328 345 346 # 435 458 554 1045 1425 # # Bce83I*: # [CTTGAG] # 368 383 449 947 3167 # # BceAI: # [ACGGC] # 85 194 761 1140 1249 1885 # # BcgI: # [CGANNNNNNTGC] # 34 1030 4250 # # BciVI: # [GTATCC] # 213 1473 3628 # # BclI: # [TGATCA] # 18 124 1230 1425 2517 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 34 34 34 34 34 34 # 54 257 445 499 668 1485 # 1702 # # BetI*: # [WCCGGW] # 120 249 571 1563 2811 # # BfiI: # [ACTGGG] # 309 5005 # # BinI*: # [GGATC] # 13 85 248 336 507 527 # 626 1439 1533 # # BisI: # [GCNGC] # 1178 1618 2518 # # BlsI: # [GCNGC] # 1179 1617 2518 # # BmeT110I: # [CYCGRG] # 1692 3622 # # BmgT120I: # [GGNCC] # 373 668 1637 2636 # # BmtI: # [GCTAGC] # 1887 3427 # # BsaAI: # [YACGTR] # 99 961 1948 2306 # # BsaBI: # [GATNNNNATC] # 6 621 1975 2712 # # BseBI: # [CCWGG] # 9 17 173 428 528 713 # 1488 1958 # # BseGI: # [GGATG] # 30 329 705 728 730 900 # 914 978 # # BseMII: # [CTCAG] # 61 258 400 1933 2662 # # BseRI: # [GAGGAG] # 203 5111 # # BseSI: # [GKGCMC] # 306 876 1732 2400 # # BsgI: # [GTGCAG] # 2263 3051 # # BsiI*: # [CACGAG] # 2547 2767 # # BsiYI*: # [CCNNNNNNNGG] # 55 244 1166 3849 # # BslFI: # [GGGAC] # 61 128 236 503 858 1733 # 1795 # # BsmAI: # [GTCTC] # 49 56 92 157 165 253 # 595 1193 1199 1555 # # BsmI: # [GAATGC] # 49 710 1794 2761 # # Bsp1407I: # [TGTACA] # 2014 3300 # # BspCNI: # [CTCAG] # 61 259 401 1931 2662 # # BspHI: # [TCATGA] # 1043 1757 2514 # # BspLU11I*: # [ACATGT] # 1936 3378 # # BspMI: # [ACCTGC] # 135 228 2084 2867 # # BsrDI: # [GCAATG] # 60 73 2347 2834 # # BsrI: # [ACTGG] # 197 304 419 495 634 790 # 1094 1381 # # BssKI: # [CCNGG] # 9 17 171 428 528 715 # 1488 1958 # # BssNAI: # [GTATAC] # 96 5218 # # BstEII: # [GGTNACC] # 428 4886 # # BstKTI: # [GATC] # 7 16 18 36 43 49 # 66 70 84 93 124 136 # 163 164 199 228 248 315 # 336 367 371 437 519 581 # 644 # # BtgZI: # [GCGATG] # 427 1286 3601 # # BtrI: # [CACGTC] # 332 4982 # # BtsI: # [GCAGTG] # 20 704 2251 2339 # # Cac8I: # [GCNNGC] # 22 87 279 286 288 1298 # 3054 # # Cfr10I: # [RCCGGY] # 2503 2811 # # ClaI: # [ATCGAT] # 361 434 1217 1598 1704 # # Csp6I: # [GTAC] # 10 11 15 23 27 27 # 28 33 42 47 55 60 # 63 74 80 105 107 114 # 120 156 161 229 319 356 # 428 590 667 671 696 # # CspCI: # [CAANNNNNGTGG] # 35 504 4775 # # CviAII: # [CATG] # 23 24 40 55 57 62 # 101 122 132 138 160 165 # 185 194 205 249 262 269 # 295 322 337 348 374 567 # 628 # # CviJI: # [RGCY] # 4 6 9 19 22 24 # 26 37 54 61 66 67 # 71 75 77 100 102 102 # 108 110 121 122 130 134 # 137 153 173 177 224 226 # 255 263 273 330 387 493 # 576 # # CviRI*: # [TGCA] # 25 43 44 50 76 94 # 97 104 152 156 186 222 # 258 261 296 326 420 456 # 627 656 765 # # DdeI: # [CTNAG] # 6 23 61 68 75 131 # 141 266 477 815 1066 2185 # # DpnI: # [GATC] # 7 16 18 36 43 49 # 66 70 84 92 124 136 # 163 164 200 228 248 315 # 336 367 371 437 519 581 # 644 # # DraIII: # [CACNNNGTG] # 261 2294 2759 # # DrdI: # [GACNNNNNNGTC] # 949 1275 3090 # # DsaI*: # [CCRYGG] # 2271 3043 # # Eam1105I: # [GACNNNNNGTC] # 405 2055 2854 # # EciI: # [GGCGGA] # 1981 3333 # # Ecl136II: # [GAGCTC] # 743 4571 # # Eco31I: # [GGTCTC] # 92 5222 # # Eco57I: # [CTGAAG] # 209 267 4838 # # Eco57MI: # [CTGRAG] # 209 267 757 4081 # # EcoP15I: # [CAGCAG] # 805 1607 2902 # # EcoRII: # [CCWGG] # 9 17 171 428 528 715 # 1488 1958 # # EcoRV: # [GATATC] # 1203 4111 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 183 206 2023 # 2806 # # FatI: # [CATG] # 23 24 39 55 57 62 # 101 122 132 138 160 165 # 185 194 205 249 262 269 # 295 322 338 348 374 567 # 628 # # FnuDII*: # [CGCG] # 1042 2097 2175 # # FokI: # [GGATG] # 30 329 698 715 730 880 # 934 998 # # GlaI: # [GCGC] # 48 5266 # # GsuI: # [CTGGAG] # 757 4557 # # HaeII: # [RGCGCY] # 50 5264 # # HaeIII: # [GGCC] # 292 383 621 4018 # # HgiAI*: # [GWGCWC] # 252 624 741 1659 2038 # # HgiCI*: # [GGYRCC] # 1503 1514 2297 # # HgiJII*: # [GRGCYC] # 741 4573 # # HhaI: # [GCGC] # 49 5265 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 0 0 0 0 7 7 # 7 7 13 18 25 25 # 32 32 32 32 32 32 # 32 32 32 32 32 32 # 34 36 51 55 106 112 # 112 229 291 433 571 605 # 2186 # # Hin4II*: # [CCTTC] # 133 200 346 666 704 1021 # 2244 # # Hin6I: # [GCGC] # 47 5267 # # HindII: # [GTYRAC] # 14 15 340 379 760 822 # 919 2065 # # HindIII: # [AAGCTT] # 1513 3801 # # HinfI: # [GANTC] # 4 8 9 24 36 38 # 46 67 101 108 109 129 # 132 165 196 234 246 252 # 261 300 441 489 603 1316 # # HpaI: # [GTTAAC] # 340 2459 2515 # # HpaII: # [CCGG] # 121 249 571 1563 2810 # # HphI: # [GGTGA] # 24 69 79 100 116 275 # 277 278 339 341 344 609 # 1066 1397 # # Hpy166II: # [GTNNAC] # 14 15 22 31 66 67 # 79 88 96 122 141 149 # 156 295 305 330 337 379 # 517 582 645 878 # # Hpy178III*: # [TCNNGA] # 18 35 49 52 80 87 # 88 95 102 158 175 221 # 245 266 330 527 588 627 # 728 843 # # Hpy188I: # [TCNGA] # 10 18 18 37 38 38 # 42 43 43 63 74 90 # 108 117 127 175 202 246 # 279 316 333 342 389 433 # 456 1277 # # Hpy99I: # [CGWCG] # 823 1286 3205 # # KpnI: # [GGTACC] # 2293 3021 # # Ksp632I*: # [CTCTTC] # 41 43 138 2188 2904 # # MaeI: # [CTAG] # 51 56 60 82 103 205 # 235 296 444 455 557 665 # 900 1205 # # MaeII: # [ACGT] # 18 30 100 109 137 142 # 255 262 301 365 469 678 # 762 777 909 # # MaeIII: # [GTNAC] # 11 16 19 60 129 188 # 230 268 269 290 363 583 # 626 712 718 832 # # MboI: # [GATC] # 7 16 18 36 43 49 # 66 70 84 90 124 136 # 163 164 202 228 248 315 # 336 367 371 437 519 581 # 644 # # MboII: # [GAAGA] # 10 28 44 56 67 80 # 93 98 254 277 345 362 # 384 406 445 504 504 516 # 841 # # McrI*: # [CGRYCG] # 2104 3210 # # MlyI: # [GAGTC] # 842 1197 1328 1947 # # MmeI: # [TCCRAC] # 651 1375 3288 # # MnlI: # [CCTC] # 21 37 40 44 45 47 # 58 72 81 84 108 108 # 126 146 161 184 223 224 # 280 291 316 346 393 613 # 1266 # # MseI: # [TTAA] # 8 11 15 18 23 29 # 31 36 42 49 51 52 # 62 68 91 92 104 108 # 141 145 149 155 156 159 # 174 175 190 210 217 237 # 265 301 315 320 324 362 # 429 # # MslI: # [CAYNNNNRTG] # 41 195 203 417 656 1035 # 1294 1473 # # MwoI: # [GCNNNNNNNGC] # 106 1077 1279 2852 # # NdeI: # [CATATG] # 96 108 432 693 804 1209 # 1972 # # NheI: # [GCTAGC] # 1883 3431 # # NlaIII: # [CATG] # 23 24 43 55 57 62 # 101 122 132 138 160 165 # 185 194 205 249 262 269 # 295 322 334 348 374 567 # 628 # # NlaIV: # [GGNNCC] # 127 159 164 318 374 687 # 689 1142 1654 # # NspBII*: # [CMGCKG] # 184 1236 3894 # # NspI: # [RCATGY] # 138 194 322 334 640 719 # 1162 1805 # # PflMI: # [CCANNNNNTGG] # 244 5070 # # PleI: # [GAGTC] # 842 1197 1328 1947 # # PpiI: # [GAACNNNNNCTC] # 32 2445 2837 # # PshAI: # [GACNNNNGTC] # 2262 3052 # # PsiI: # [TTATAA] # 1549 3765 # # PvuII: # [CAGCTG] # 184 1236 3894 # # RsaI: # [GTAC] # 10 11 15 23 27 27 # 28 33 42 47 55 60 # 63 74 80 105 107 114 # 120 156 161 230 319 356 # 428 590 667 670 696 # # SacI: # [GAGCTC] # 741 4573 # # SalI: # [GTCGAC] # 357 4957 # # ScaI: # [AGTACT] # 414 1574 3326 # # ScrFI: # [CCNGG] # 9 17 173 428 528 713 # 1488 1958 # # SduI: # [GDGCHC] # 252 306 624 741 1659 1732 # # SecI*: # [CCNNGG] # 172 428 1671 3043 # # SetI: # [ASST] # 4 4 5 6 6 6 # 8 9 9 11 11 12 # 12 14 15 15 15 16 # 18 18 19 20 21 23 # 24 25 26 27 30 31 # 33 36 38 39 41 43 # 46 47 47 48 49 51 # 55 56 58 65 69 69 # 70 78 86 87 91 122 # 123 124 135 143 151 164 # 165 166 170 180 183 203 # 206 210 210 272 301 324 # # SexAI: # [ACCWGGT] # 732 1497 3085 # # SfaNI: # [GCATC] # 450 1441 1476 1947 # # SfeI*: # [CTRYAG] # 325 4989 # # SmlI: # [CTYRAG] # 51 347 404 464 563 643 # 932 1910 # # SnaBI: # [TACGTA] # 99 961 4254 # # SpeI: # [ACTAGT] # 456 4858 # # SphI: # [GCATGC] # 2262 3052 # # SspI: # [AATATT] # 403 910 1194 1353 1454 # # TaiI: # [ACGT] # 18 33 97 109 137 142 # 255 262 301 365 469 678 # 762 777 909 # # TaqI: # [TCGA] # 14 45 88 112 146 250 # 268 316 337 352 434 821 # 903 1228 # # TaqII: # [CACCCA] # 156 366 412 516 633 882 # 908 1441 # # TatI: # [WGTACW] # 28 160 194 412 414 672 # 1160 2274 # # TfiI: # [GAWTC] # 4 8 9 38 60 67 # 108 109 129 132 147 165 # 196 234 246 300 441 489 # 513 603 1316 # # TseI: # [GCWGC] # 1177 1619 2518 # # TsoI: # [TARCCA] # 98 170 410 911 1068 1083 # 1574 # # Tsp45I: # [GTSAC] # 140 230 269 350 363 870 # 1458 1634 # # Tsp4CI*: # [ACNGT] # 12 29 69 75 117 189 # 190 198 206 235 251 262 # 335 343 393 394 743 1273 # # TspDTI: # [ATGAA] # 3 6 12 15 17 21 # 23 28 54 57 67 86 # 89 92 115 118 120 150 # 184 266 282 336 372 385 # 616 840 960 # # TspEI: # [AATT] # 4 5 5 9 9 10 # 13 13 20 23 26 27 # 30 31 42 45 56 60 # 62 62 64 66 67 67 # 76 83 86 96 97 101 # 108 126 130 152 160 165 # 171 193 219 228 231 254 # 272 305 390 394 461 # # TspGWI: # [ACGGA] # 83 132 162 215 291 364 # 411 462 801 928 1465 # # TspRI: # [CASTGNN] # 12 13 204 294 413 711 # 758 976 1933 # # TstI: # [CACNNNNNNTCC] # 32 383 4899 # # VspI: # [ATTAAT] # 497 522 716 1753 1826 # # XbaI: # [TCTAGA] # 2282 3032 # # XcmI: # [CCANNNNNNNNNTGG] # 175 713 2195 2231 # # XhoII: # [RGATCY] # 90 540 1434 3250 # # XmnI: # [GAANNNNTTC] # 292 687 2162 2173 # # ZraI: # [GACGTC] # 1279 4035 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 5314 # Reported_sequences: 1 # Reported_hitcount: 1180 #---------------------------------------