Restriction Fragments for YCL001W-B



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YCL001W-B on chromosome III from coordinates 113770 to 114024.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30493.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 44 47 + FatI CATG 43 47 . . 44 47 + NlaIII CATG 47 43 . . 44 47 + CviAII CATG 44 46 . . 53 56 + Hin6I GCGC 53 55 . . 53 56 + GlaI GCGC 54 54 . . 53 56 + HhaI GCGC 55 53 . . 54 57 + FnuDII* CGCG 55 55 . . 58 62 + MboII GAAGA 70 69 . . 55 58 - AciI CCGC 55 57 . . 66 77 + BcgI CGANNNNNNTGC 55 53 89 87 72 75 + SetI ASST 75 71 . . 72 75 + CviJI RGCY 73 73 . . 72 75 + AluI AGCT 73 73 . . 73 77 + BlsI GCNGC 75 74 . . 73 77 + BisI GCNGC 74 75 . . 73 77 + TseI GCWGC 73 76 . . 75 78 + CviRI* TGCA 76 76 . . 73 77 - BbvI GCAGC 60 64 . . 80 83 + TspEI AATT 79 83 . . 87 90 + CviRI* TGCA 88 88 . . 85 89 - SfaNI GCATC 75 79 . . 116 121 + Hpy166II GTNNAC 118 118 . . 117 122 + TatI WGTACW 117 121 . . 118 121 + Csp6I GTAC 118 120 . . 118 121 + RsaI GTAC 119 119 . . 132 136 + Hpy99I CGWCG 136 131 . . 134 137 + TaiI ACGT 137 133 . . 134 137 + SetI ASST 137 133 . . 134 137 + MaeII ACGT 134 136 . . 133 138 - BtrI CACGTC 135 135 . . 144 147 + TaiI ACGT 147 143 . . 144 147 + SetI ASST 147 143 . . 144 147 + MaeII ACGT 144 146 . . 155 158 + SetI ASST 158 154 . . 157 160 + MaeI CTAG 157 159 . . 161 164 + SetI ASST 164 160 . . 195 200 + SspI AATATT 197 197 . . 202 205 + Csp6I GTAC 202 204 . . 191 202 - BcgI CGANNNNNNTGC 214 212 180 178 202 205 + RsaI GTAC 203 203 . . 205 209 + DdeI CTNAG 205 208 . . 205 209 + BspCNI CTCAG 218 216 . . 205 209 + BseMII CTCAG 219 217 . . 208 213 + TatI WGTACW 208 212 . . 208 213 + ScaI AGTACT 210 210 . . 209 212 + Csp6I GTAC 209 211 . . 209 212 + RsaI GTAC 210 210 . . 223 227 + Tsp4CI* ACNGT 225 224 . . 224 230 + TspRI CASTGNN 230 221 . . #--------------------------------------- # # # AciI: # [CCGC] # 55 200 # # AluI: # [AGCT] # 73 182 # # BbvI: # [GCAGC] # 60 195 # # BcgI: # [CGANNNNNNTGC] # 34 34 41 55 91 # # BisI: # [GCNGC] # 74 181 # # BlsI: # [GCNGC] # 75 180 # # BseMII: # [CTCAG] # 36 219 # # BspCNI: # [CTCAG] # 37 218 # # BtrI: # [CACGTC] # 120 135 # # Csp6I: # [GTAC] # 7 46 84 118 # # CviAII: # [CATG] # 44 211 # # CviJI: # [RGCY] # 73 182 # # CviRI*: # [TGCA] # 12 76 167 # # DdeI: # [CTNAG] # 50 205 # # FatI: # [CATG] # 43 212 # # FnuDII*: # [CGCG] # 55 200 # # GlaI: # [GCGC] # 54 201 # # HhaI: # [GCGC] # 55 200 # # Hin6I: # [GCGC] # 53 202 # # Hpy166II: # [GTNNAC] # 118 137 # # Hpy99I: # [CGWCG] # 119 136 # # MaeI: # [CTAG] # 98 157 # # MaeII: # [ACGT] # 10 111 134 # # MboII: # [GAAGA] # 70 185 # # NlaIII: # [CATG] # 47 208 # # RsaI: # [GTAC] # 7 45 84 119 # # ScaI: # [AGTACT] # 45 210 # # SetI: # [ASST] # 6 10 11 62 75 91 # # SfaNI: # [GCATC] # 75 180 # # SspI: # [AATATT] # 58 197 # # TaiI: # [ACGT] # 10 108 137 # # TatI: # [WGTACW] # 47 91 117 # # TseI: # [GCWGC] # 73 182 # # Tsp4CI*: # [ACNGT] # 30 225 # # TspEI: # [AATT] # 79 176 # # TspRI: # [CASTGNN] # 25 230 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 255 # Reported_sequences: 1 # Reported_hitcount: 49 #---------------------------------------