Restriction Fragments for YBRWdelta16



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YBRWdelta16 on chromosome II from coordinates 327390 to 327704.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.17969.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 12 15 + SetI ASST 15 11 . . 13 17 + Hin4II* CCTTC 23 22 . . 15 19 - TspDTI ATGAA 5 3 . . 22 27 + Hpy166II GTNNAC 24 24 . . 22 27 + AccI GTMKAC 23 25 . . 27 31 - BsrI ACTGG 27 25 . . 58 62 + Tsp4CI* ACNGT 60 59 . . 59 65 + TspRI CASTGNN 65 56 . . 91 95 + Tsp4CI* ACNGT 93 92 . . 98 102 + Hpy188I TCNGA 100 99 . . 112 115 + SetI ASST 115 111 . . 112 115 + CviJI RGCY 113 113 . . 112 115 + AluI AGCT 113 113 . . 140 144 + HphI GGTGA 152 151 . . 141 145 + Tsp45I GTSAC 140 145 . . 141 145 + MaeIII GTNAC 140 145 . . 132 143 - TstI CACNNNNNNTCC 156 151 124 119 138 143 - TaqII CACCCA 128 126 . . 173 176 + CviJI RGCY 174 174 . . 181 186 + SnaBI TACGTA 183 183 . . 181 186 + BsaAI YACGTR 183 183 . . 182 185 + TaiI ACGT 185 181 . . 182 185 + SetI ASST 185 181 . . 182 185 + MaeII ACGT 182 184 . . 207 212 + ApoI RAATTY 207 211 . . 208 211 + TspEI AATT 207 211 . . 215 220 + BciVI GTATCC 226 225 . . 219 223 + BssKI CCNGG 218 223 . . 219 223 + BseBI CCWGG 220 221 . . 219 224 + SecI* CCNNGG 219 223 . . 219 223 + ScrFI CCNGG 220 221 . . 219 223 + EcoRII CCWGG 218 223 . . 225 229 + MboII GAAGA 237 236 . . 230 233 + SetI ASST 233 229 . . 258 263 + PsiI TTATAA 260 260 . . 263 266 + SetI ASST 266 262 . . 266 269 + MseI TTAA 266 268 . . 288 291 + TspEI AATT 287 291 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 23 292 # # AluI: # [AGCT] # 113 202 # # ApoI: # [RAATTY] # 108 207 # # BciVI: # [GTATCC] # 89 226 # # BsaAI: # [YACGTR] # 132 183 # # BseBI: # [CCWGG] # 95 220 # # BsrI: # [ACTGG] # 27 288 # # BssKI: # [CCNGG] # 97 218 # # CviJI: # [RGCY] # 61 113 141 # # EcoRII: # [CCWGG] # 97 218 # # Hin4II*: # [CCTTC] # 23 292 # # HphI: # [GGTGA] # 152 163 # # Hpy166II: # [GTNNAC] # 24 291 # # Hpy188I: # [TCNGA] # 100 215 # # MaeII: # [ACGT] # 133 182 # # MaeIII: # [GTNAC] # 140 175 # # MboII: # [GAAGA] # 78 237 # # MseI: # [TTAA] # 49 266 # # PsiI: # [TTATAA] # 55 260 # # ScrFI: # [CCNGG] # 95 220 # # SecI*: # [CCNNGG] # 96 219 # # SetI: # [ASST] # 15 33 48 49 70 100 # # SnaBI: # [TACGTA] # 132 183 # # TaiI: # [ACGT] # 130 185 # # TaqII: # [CACCCA] # 128 187 # # Tsp45I: # [GTSAC] # 140 175 # # Tsp4CI*: # [ACNGT] # 33 60 222 # # TspDTI: # [ATGAA] # 5 310 # # TspEI: # [AATT] # 28 80 207 # # TspRI: # [CASTGNN] # 65 250 # # TstI: # [CACNNNNNNTCC] # 32 124 159 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 315 # Reported_sequences: 1 # Reported_hitcount: 38 #---------------------------------------