Restriction Fragments for DPB3/YBR278W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

DPB3/YBR278W on chromosome II from coordinates 760294 to 760899.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.29322.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + MmeI TCCRAC 29 27 . . 9 13 + DdeI CTNAG 9 12 . . 14 17 + MseI TTAA 14 16 . . 27 30 + SetI ASST 30 26 . . 54 58 + MboII GAAGA 66 65 . . 79 84 + SnaBI TACGTA 81 81 . . 79 84 + BsaAI YACGTR 81 81 . . 80 83 + TaiI ACGT 83 79 . . 80 83 + SetI ASST 83 79 . . 80 83 + MaeII ACGT 80 82 . . 84 87 + TspEI AATT 83 87 . . 99 102 + SetI ASST 102 98 . . 99 102 + CviJI RGCY 100 100 . . 99 102 + AluI AGCT 100 100 . . 100 110 + MwoI GCNNNNNNNGC 106 103 . . 110 115 + McrI* CGRYCG 113 111 . . 113 116 + AciI CCGC 113 115 . . 115 125 + MwoI GCNNNNNNNGC 121 118 . . 115 125 + BstAPI GCANNNNNTGC 121 118 . . 115 120 - BsmI GAATGC 115 113 . . 121 125 + BlsI GCNGC 123 122 . . 121 125 + BisI GCNGC 122 123 . . 121 125 + TseI GCWGC 121 124 . . 125 129 + DdeI CTNAG 125 128 . . 121 125 - BbvI GCAGC 108 112 . . 122 127 - EcoP15I CAGCAG 94 96 . . 125 129 - BspCNI CTCAG 117 115 . . 125 129 - BseMII CTCAG 116 114 . . 135 140 + TatI WGTACW 135 139 . . 135 140 + Bsp1407I TGTACA 135 139 . . 136 139 + Csp6I GTAC 136 138 . . 136 139 + RsaI GTAC 137 137 . . 141 145 + TfiI GAWTC 141 144 . . 141 145 + HinfI GANTC 141 144 . . 147 151 + Hpy99I CGWCG 151 146 . . 149 152 + TaqI TCGA 149 151 . . 151 155 + MboII GAAGA 163 162 . . 154 158 + TfiI GAWTC 154 157 . . 154 158 + HinfI GANTC 154 157 . . 165 169 + DdeI CTNAG 165 168 . . 177 182 + EcoRI GAATTC 177 181 . . 177 182 + ApoI RAATTY 177 181 . . 178 181 + TspEI AATT 177 181 . . 180 185 + AsuII TTCGAA 181 183 . . 181 184 + TaqI TCGA 181 183 . . 196 199 + CviJI RGCY 197 197 . . 205 208 + MseI TTAA 205 207 . . 208 211 + CviJI RGCY 209 209 . . 213 218 + ApoI RAATTY 213 217 . . 214 217 + TspEI AATT 213 217 . . 218 223 + SfeI* CTRYAG 218 222 . . 223 227 + MboII GAAGA 235 234 . . 233 236 + TaqI TCGA 233 235 . . 247 250 + TspEI AATT 246 250 . . 253 256 + SetI ASST 256 252 . . 258 263 + XbaI TCTAGA 258 262 . . 258 263 + Hpy178III* TCNNGA 259 261 . . 259 262 + MaeI CTAG 259 261 . . 264 268 + FokI GGATG 277 281 . . 264 268 + BseGI GGATG 270 268 . . 262 265 - MnlI CCTC 255 254 . . 265 269 - SfaNI GCATC 255 259 . . 272 275 + MseI TTAA 272 274 . . 280 285 + Eco57MI CTGRAG 301 299 . . 280 285 + Eco57I CTGAAG 301 299 . . 282 286 + MboII GAAGA 294 293 . . 285 289 + MboII GAAGA 297 296 . . 293 296 + Hin6I GCGC 293 295 . . 293 296 + GlaI GCGC 294 294 . . 293 296 + HhaI GCGC 295 293 . . 299 302 + TaqI TCGA 299 301 . . 306 310 + MboII GAAGA 318 317 . . 306 311 - Ksp632I* CTCTTC 301 304 . . 320 325 + NspI RCATGY 324 320 . . 321 324 + FatI CATG 320 324 . . 321 324 + NlaIII CATG 324 320 . . 321 324 + CviAII CATG 321 323 . . 323 326 + CviRI* TGCA 324 324 . . 328 332 + BssKI CCNGG 327 332 . . 328 331 + HpaII CCGG 328 330 . . 328 332 + ScrFI CCNGG 329 330 . . 328 332 + CauII* CCSGG 329 330 . . 333 337 + Hpy188I TCNGA 335 334 . . 324 335 - BcgI CGANNNNNNTGC 347 345 313 311 332 337 - MmeI TCCRAC 313 311 . . 340 343 + DpnI GATC 341 341 . . 340 343 + BstKTI GATC 342 340 . . 340 343 + MboI GATC 339 343 . . 342 347 + Hpy178III* TCNNGA 343 345 . . 347 350 + SetI ASST 350 346 . . 346 349 - MnlI CCTC 339 338 . . 358 362 + MboII GAAGA 370 369 . . 362 365 + CviJI RGCY 363 363 . . 363 373 + MwoI GCNNNNNNNGC 369 366 . . 358 363 - Ksp632I* CTCTTC 353 356 . . 358 364 - SapI GCTCTTC 353 356 . . 368 371 + TspEI AATT 367 371 . . 371 374 + CviRI* TGCA 372 372 . . 373 382 + MslI CAYNNNNRTG 377 377 . . 373 376 + FatI CATG 372 376 . . 373 376 + NlaIII CATG 376 372 . . 373 376 + CviAII CATG 373 375 . . 378 382 + FokI GGATG 391 395 . . 378 382 + BseGI GGATG 384 382 . . 376 379 - MnlI CCTC 369 368 . . 382 386 - BccI CCATC 376 377 . . 391 394 - MnlI CCTC 384 383 . . 394 398 + MboII GAAGA 406 405 . . 397 401 + MboII GAAGA 409 408 . . 400 404 + MboII GAAGA 412 411 . . 403 407 + MboII GAAGA 415 414 . . 406 410 + MboII GAAGA 418 417 . . 406 411 + BbvII* GAAGAC 413 417 . . 409 419 + Hin4I GAYNNNNNVTC 400 395 432 427 413 416 + SetI ASST 416 412 . . 412 415 - MnlI CCTC 405 404 . . 415 420 + BciVI GTATCC 426 425 . . 418 422 + Hpy188I TCNGA 420 419 . . 409 419 - Hin4I GAYNNNNNVTC 432 427 400 395 421 425 + MboII GAAGA 433 432 . . 424 428 + MboII GAAGA 436 435 . . 427 431 + MboII GAAGA 439 438 . . 427 439 + BarI GAAGNNNNNNTAC 419 414 451 446 430 435 + SduI GDGCHC 434 430 . . 430 435 + HgiJII* GRGCYC 434 430 . . 431 434 + CviJI RGCY 432 432 . . 427 432 - Ksp632I* CTCTTC 422 425 . . 427 433 - SapI GCTCTTC 422 425 . . 436 441 + Hpy166II GTNNAC 438 438 . . 436 439 + Csp6I GTAC 436 438 . . 436 439 + RsaI GTAC 437 437 . . 443 447 + TspDTI ATGAA 458 456 . . 445 449 + MboII GAAGA 457 456 . . 445 454 + XmnI GAANNNNTTC 449 449 . . 446 456 + FalI AAGNNNNNCTT 437 432 469 464 453 458 + Hpy178III* TCNNGA 454 456 . . 446 456 - FalI AAGNNNNNCTT 469 464 437 432 469 472 + DpnI GATC 470 470 . . 469 472 + BstKTI GATC 471 469 . . 469 472 + MboI GATC 468 472 . . 488 502 + XcmI CCANNNNNNNNNTGG 495 494 . . 491 494 + FnuDII* CGCG 492 492 . . 492 495 + Hin6I GCGC 492 494 . . 492 495 + GlaI GCGC 493 493 . . 492 495 + HhaI GCGC 494 492 . . 494 499 + BtsI GCAGTG 501 499 . . 495 501 + TspRI CASTGNN 501 492 . . 502 507 + Cac8I GCNNGC 504 504 . . 504 509 + HindIII AAGCTT 504 508 . . 505 508 + SetI ASST 508 504 . . 505 508 + CviJI RGCY 506 506 . . 505 508 + AluI AGCT 506 506 . . 506 511 + Cac8I GCNNGC 508 508 . . 514 517 + TaqI TCGA 514 516 . . 514 519 + Hpy178III* TCNNGA 515 517 . . 518 522 + TfiI GAWTC 518 521 . . 518 522 + HinfI GANTC 518 521 . . 522 526 - BsrI ACTGG 522 520 . . 533 536 + AciI CCGC 533 535 . . 538 541 + MaeI CTAG 538 540 . . 542 545 + TaiI ACGT 545 541 . . 542 545 + SetI ASST 545 541 . . 542 545 + MaeII ACGT 542 544 . . 556 561 + MmeI TCCRAC 581 579 . . 556 560 + Hpy188I TCNGA 558 557 . . 559 563 + HinfI GANTC 559 562 . . 561 566 + Ksp632I* CTCTTC 567 570 . . 559 563 - PleI GAGTC 553 554 . . 559 563 - MlyI GAGTC 553 553 . . 565 569 + Hpy188I TCNGA 567 566 . . 562 566 - MboII GAAGA 554 553 . . 568 573 + EcoRV GATATC 570 570 . . 572 575 + TaqI TCGA 572 574 . . 577 582 + Hpy166II GTNNAC 579 579 . . 577 582 + HindII GTYRAC 579 579 . . 598 601 + DpnI GATC 599 599 . . 598 601 + BstKTI GATC 600 598 . . 598 601 + MboI GATC 597 601 . . 602 606 + DdeI CTNAG 602 605 . . 598 602 - BinI* GGATC 592 593 . . #--------------------------------------- # # # AciI: # [CCGC] # 73 113 420 # # AluI: # [AGCT] # 100 100 406 # # ApoI: # [RAATTY] # 36 177 393 # # AsuII: # [TTCGAA] # 181 425 # # BarI: # [GAAGNNNNNNTAC] # 32 155 419 # # BbvI: # [GCAGC] # 108 498 # # BbvII*: # [GAAGAC] # 193 413 # # BccI: # [CCATC] # 230 376 # # BcgI: # [CGANNNNNNTGC] # 34 259 313 # # BciVI: # [GTATCC] # 180 426 # # BinI*: # [GGATC] # 14 592 # # BisI: # [GCNGC] # 122 484 # # BlsI: # [GCNGC] # 123 483 # # BsaAI: # [YACGTR] # 81 525 # # BseGI: # [GGATG] # 114 222 270 # # BseMII: # [CTCAG] # 116 490 # # BsmI: # [GAATGC] # 115 491 # # Bsp1407I: # [TGTACA] # 135 471 # # BspCNI: # [CTCAG] # 117 489 # # BsrI: # [ACTGG] # 84 522 # # BssKI: # [CCNGG] # 279 327 # # BstAPI: # [GCANNNNNTGC] # 121 485 # # BstKTI: # [GATC] # 6 129 129 342 # # BtsI: # [GCAGTG] # 105 501 # # Cac8I: # [GCNNGC] # 4 98 504 # # CauII*: # [CCSGG] # 277 329 # # Csp6I: # [GTAC] # 136 170 300 # # CviAII: # [CATG] # 52 233 321 # # CviJI: # [RGCY] # 12 69 74 97 100 100 # 154 # # CviRI*: # [TGCA] # 48 234 324 # # DdeI: # [CTNAG] # 4 9 40 116 437 # # DpnI: # [GATC] # 7 129 129 341 # # Eco57I: # [CTGAAG] # 301 305 # # Eco57MI: # [CTGRAG] # 301 305 # # EcoP15I: # [CAGCAG] # 94 512 # # EcoRI: # [GAATTC] # 177 429 # # EcoRV: # [GATATC] # 36 570 # # FalI: # [AAGNNNNNCTT] # 0 0 32 137 437 # # FatI: # [CATG] # 52 234 320 # # FnuDII*: # [CGCG] # 114 492 # # FokI: # [GGATG] # 114 215 277 # # GlaI: # [GCGC] # 113 199 294 # # HgiJII*: # [GRGCYC] # 172 434 # # HhaI: # [GCGC] # 112 199 295 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 174 400 # # Hin6I: # [GCGC] # 114 199 293 # # HindII: # [GTYRAC] # 27 579 # # HindIII: # [AAGCTT] # 102 504 # # HinfI: # [GANTC] # 13 41 47 141 364 # # HpaII: # [CCGG] # 278 328 # # Hpy166II: # [GTNNAC] # 27 141 438 # # Hpy178III*: # [TCNNGA] # 61 84 91 111 259 # # Hpy188I: # [TCNGA] # 9 39 85 138 335 # # Hpy99I: # [CGWCG] # 151 455 # # Ksp632I*: # [CTCTTC] # 39 52 69 145 301 # # MaeI: # [CTAG] # 68 259 279 # # MaeII: # [ACGT] # 64 80 462 # # MboI: # [GATC] # 9 129 129 339 # # MboII: # [GAAGA] # 3 3 3 3 3 3 # 3 15 18 21 36 52 # 52 59 66 72 97 97 # # McrI*: # [CGRYCG] # 113 493 # # MlyI: # [GAGTC] # 53 553 # # MmeI: # [TCCRAC] # 25 29 268 284 # # MnlI: # [CCTC] # 15 21 30 84 201 255 # # MseI: # [TTAA] # 14 67 191 334 # # MslI: # [CAYNNNNRTG] # 229 377 # # MwoI: # [GCNNNNNNNGC] # 15 106 237 248 # # NlaIII: # [CATG] # 52 230 324 # # NspI: # [RCATGY] # 282 324 # # PleI: # [GAGTC] # 53 553 # # RsaI: # [GTAC] # 137 169 300 # # SapI: # [GCTCTTC] # 69 184 353 # # ScrFI: # [CCNGG] # 277 329 # # SduI: # [GDGCHC] # 172 434 # # SetI: # [ASST] # 19 30 37 53 61 66 # 92 94 154 # # SfaNI: # [GCATC] # 255 351 # # SfeI*: # [CTRYAG] # 218 388 # # SnaBI: # [TACGTA] # 81 525 # # TaiI: # [ACGT] # 61 83 462 # # TaqI: # [TCGA] # 32 34 52 58 66 149 # 215 # # TatI: # [WGTACW] # 135 471 # # TfiI: # [GAWTC] # 13 88 141 364 # # TseI: # [GCWGC] # 121 485 # # TspDTI: # [ATGAA] # 148 458 # # TspEI: # [AATT] # 33 36 83 94 121 239 # # TspRI: # [CASTGNN] # 105 501 # # XbaI: # [TCTAGA] # 258 348 # # XcmI: # [CCANNNNNNNNNTGG] # 111 495 # # XmnI: # [GAANNNNTTC] # 157 449 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 606 # Reported_sequences: 1 # Reported_hitcount: 180 #---------------------------------------