Restriction Fragments for IFA38/YBR159W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

IFA38/YBR159W on chromosome II from coordinates 558685 to 559728.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.20446.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 14 + CviRI* TGCA 12 12 . . 17 20 + SetI ASST 20 16 . . 17 20 + CviJI RGCY 18 18 . . 17 20 + AluI AGCT 18 18 . . 21 26 + Hpy178III* TCNNGA 22 24 . . 27 30 + CviJI RGCY 28 28 . . 25 28 - MnlI CCTC 18 17 . . 28 33 - GsaI CCCAGC 32 28 . . 28 33 - BseYI CCCAGC 28 32 . . 43 46 + SetI ASST 46 42 . . 77 80 + CviJI RGCY 78 78 . . 88 91 + MseI TTAA 88 90 . . 94 99 + TatI WGTACW 94 98 . . 94 99 + Bsp1407I TGTACA 94 98 . . 95 98 + Csp6I GTAC 95 97 . . 95 98 + RsaI GTAC 96 96 . . 99 104 + AclI AACGTT 100 102 . . 100 103 + TaiI ACGT 103 99 . . 100 103 + SetI ASST 103 99 . . 100 103 + MaeII ACGT 100 102 . . 109 113 + DdeI CTNAG 109 112 . . 120 123 + SetI ASST 123 119 . . 120 123 + CviJI RGCY 121 121 . . 120 123 + AluI AGCT 121 121 . . 133 136 + DpnI GATC 134 134 . . 133 136 + BstKTI GATC 135 133 . . 133 136 + MboI GATC 132 136 . . 149 154 + EcoP15I CAGCAG 179 181 . . 157 160 + TspEI AATT 156 160 . . 161 164 + TaqI TCGA 161 163 . . 181 185 + BsrI ACTGG 186 184 . . 191 195 + Tsp4CI* ACNGT 193 192 . . 201 207 + TspRI CASTGNN 207 198 . . 202 206 + BsrI ACTGG 207 205 . . 207 210 + CviRI* TGCA 208 208 . . 212 216 + Tsp45I GTSAC 211 216 . . 212 216 + MaeIII GTNAC 211 216 . . 215 219 + Tsp4CI* ACNGT 217 216 . . 229 234 + ApoI RAATTY 229 233 . . 230 233 + TspEI AATT 229 233 . . 236 239 + MaeI CTAG 236 238 . . 252 255 + TaiI ACGT 255 251 . . 252 255 + SetI ASST 255 251 . . 252 255 + MaeII ACGT 252 254 . . 256 259 + CviJI RGCY 257 257 . . 273 276 + DpnI GATC 274 274 . . 273 276 + BstKTI GATC 275 273 . . 273 276 + MboI GATC 272 276 . . 275 280 + Hpy178III* TCNNGA 276 278 . . 276 281 + SmlI CTYRAG 276 280 . . 276 281 + XhoI CTCGAG 276 280 . . 276 281 + BmeT110I CYCGRG 277 279 . . 276 281 + AvaI CYCGRG 276 280 . . 277 280 + TaqI TCGA 277 279 . . 277 282 + Hpy178III* TCNNGA 278 280 . . 293 296 + TspEI AATT 292 296 . . 300 303 + CviJI RGCY 301 301 . . 298 301 - MnlI CCTC 291 290 . . 316 319 + MaeI CTAG 316 318 . . 319 323 + MboII GAAGA 331 330 . . 322 325 + DpnI GATC 323 323 . . 322 325 + BstKTI GATC 324 322 . . 322 331 + BsaBI GATNNNNATC 326 326 . . 322 325 + MboI GATC 321 325 . . 331 334 + FatI CATG 330 334 . . 331 334 + NlaIII CATG 334 330 . . 331 334 + CviAII CATG 331 333 . . 345 349 + TfiI GAWTC 345 348 . . 345 349 + HinfI GANTC 345 348 . . 349 352 + MaeI CTAG 349 351 . . 350 355 + TsoI TARCCA 366 364 . . 351 354 + CviJI RGCY 352 352 . . 360 365 - BsrDI GCAATG 359 357 . . 364 367 - AciI CCGC 364 366 . . 367 370 - MnlI CCTC 360 359 . . 370 380 + Hin4I GAYNNNNNVTC 361 356 393 388 376 380 + TfiI GAWTC 376 379 . . 376 380 + HinfI GANTC 376 379 . . 379 384 + MmeI TCCRAC 404 402 . . 370 380 - Hin4I GAYNNNNNVTC 393 388 361 356 386 390 + TspDTI ATGAA 401 399 . . 388 392 + TfiI GAWTC 388 391 . . 388 392 + HinfI GANTC 388 391 . . 395 398 + MseI TTAA 395 397 . . 401 404 + TspEI AATT 400 404 . . 408 413 + MstI* TGCGCA 410 410 . . 409 412 + Hin6I GCGC 409 411 . . 409 412 + GlaI GCGC 410 410 . . 409 412 + HhaI GCGC 411 409 . . 414 418 + MaeIII GTNAC 413 418 . . 424 428 + Tsp4CI* ACNGT 426 425 . . 422 426 - HphI GGTGA 414 413 . . 484 488 + MboII GAAGA 496 495 . . 484 489 - Ksp632I* CTCTTC 479 482 . . 489 493 - Hin4II* CCTTC 483 482 . . 494 497 + SetI ASST 497 493 . . 494 497 + CviJI RGCY 495 495 . . 494 497 + AluI AGCT 495 495 . . 496 500 + DdeI CTNAG 496 499 . . 502 507 + SspI AATATT 504 504 . . 522 528 + TspRI CASTGNN 528 519 . . 522 527 - BtsI GCAGTG 521 519 . . 534 539 + VspI ATTAAT 535 537 . . 535 538 + MseI TTAA 535 537 . . 537 540 + TspEI AATT 536 540 . . 552 555 + CviRI* TGCA 553 553 . . 549 554 - BsrDI GCAATG 548 546 . . 557 568 + CspCI CAANNNNNGTGG 545 543 580 578 571 575 + Tsp4CI* ACNGT 573 572 . . 579 582 + SetI ASST 582 578 . . 579 582 + CviJI RGCY 580 580 . . 579 582 + AluI AGCT 580 580 . . 599 602 + Csp6I GTAC 599 601 . . 599 602 + RsaI GTAC 600 600 . . 602 607 - BsiI* CACGAG 602 606 . . 612 615 + DpnI GATC 613 613 . . 612 615 + BstKTI GATC 614 612 . . 612 615 + MboI GATC 611 615 . . 616 619 + MseI TTAA 616 618 . . 617 622 + TsoI TARCCA 633 631 . . 620 625 + DsaI* CCRYGG 620 624 . . 620 625 + StyI CCWWGG 620 624 . . 620 625 + NcoI CCATGG 620 624 . . 620 625 + SecI* CCNNGG 620 624 . . 621 624 + FatI CATG 620 624 . . 621 624 + NlaIII CATG 624 620 . . 621 624 + CviAII CATG 621 623 . . 624 629 + SduI GDGCHC 628 624 . . 624 629 + HgiJII* GRGCYC 628 624 . . 625 628 + CviJI RGCY 626 626 . . 639 643 + Hpy188I TCNGA 641 640 . . 642 646 + TfiI GAWTC 642 645 . . 642 646 + HinfI GANTC 642 645 . . 647 661 + XcmI CCANNNNNNNNNTGG 654 653 . . 651 661 + BsiYI* CCNNNNNNNGG 657 654 . . 660 663 + CviJI RGCY 661 661 . . 659 664 - TsoI TARCCA 649 647 . . 668 672 + Tsp4CI* ACNGT 670 669 . . 669 675 + TspRI CASTGNN 675 666 . . 675 680 + AsuII TTCGAA 676 678 . . 676 679 + TaqI TCGA 676 678 . . 678 687 + XmnI GAANNNNTTC 682 682 . . 693 696 + SetI ASST 696 692 . . 711 714 + CviJI RGCY 712 712 . . 712 717 - GsaI CCCAGC 716 712 . . 712 717 - BseYI CCCAGC 712 716 . . 719 722 + TspEI AATT 718 722 . . 730 734 - SfaNI GCATC 720 724 . . 736 741 + ClaI ATCGAT 737 739 . . 737 740 + TaqI TCGA 737 739 . . 746 749 + TspEI AATT 745 749 . . 747 752 + VspI ATTAAT 748 750 . . 748 751 + MseI TTAA 748 750 . . 756 760 - TspDTI ATGAA 746 744 . . 766 770 + Tsp45I GTSAC 765 770 . . 766 770 + MaeIII GTNAC 765 770 . . 770 773 + MaeI CTAG 770 772 . . 772 775 + SetI ASST 775 771 . . 772 775 + CviJI RGCY 773 773 . . 772 775 + AluI AGCT 773 773 . . 775 778 + TaqI TCGA 775 777 . . 788 792 + Hpy188I TCNGA 790 789 . . 791 795 + MboII GAAGA 803 802 . . 792 802 + FalI AAGNNNNNCTT 783 778 815 810 794 797 + DpnI GATC 795 795 . . 794 797 + BstKTI GATC 796 794 . . 794 797 + MboI GATC 793 797 . . 802 805 + MseI TTAA 802 804 . . 792 802 - FalI AAGNNNNNCTT 815 810 783 778 807 817 + Hin4I GAYNNNNNVTC 798 793 830 825 807 810 + DpnI GATC 808 808 . . 807 810 + BstKTI GATC 809 807 . . 807 810 + MboI GATC 806 810 . . 807 811 - BinI* GGATC 801 802 . . 807 817 - Hin4I GAYNNNNNVTC 830 825 798 793 822 826 + Tsp4CI* ACNGT 824 823 . . 841 844 + MseI TTAA 841 843 . . 854 864 + MwoI GCNNNNNNNGC 860 857 . . 854 864 + BstAPI GCANNNNNTGC 860 857 . . 855 863 + AlwNI CAGNNNCTG 860 857 . . 857 861 + HgaI GACGC 866 871 . . 860 864 + BlsI GCNGC 862 861 . . 860 864 + BisI GCNGC 861 862 . . 860 864 + TseI GCWGC 860 863 . . 863 867 + TauI GCSGC 866 863 . . 863 867 + BlsI GCNGC 865 864 . . 863 867 + BisI GCNGC 864 865 . . 860 864 - BbvI GCAGC 847 851 . . 865 868 + CviJI RGCY 866 866 . . 863 866 - AciI CCGC 863 865 . . 869 874 + SmlI CTYRAG 869 873 . . 870 875 + Hpy178III* TCNNGA 871 873 . . 869 874 - Bce83I* CTTGAG 854 852 . . 893 903 + BsiYI* CCNNNNNNNGG 899 896 . . 894 904 + BsiYI* CCNNNNNNNGG 900 897 . . 899 903 + BsrI ACTGG 904 902 . . 899 904 + BfiI ACTGGG 909 908 . . 904 907 + Hin6I GCGC 904 906 . . 904 907 + GlaI GCGC 905 905 . . 904 907 + HhaI GCGC 906 904 . . 906 911 + Cac8I GCNNGC 908 908 . . 907 915 + DraIII CACNNNGTG 912 909 . . 910 915 + BtsI GCAGTG 917 915 . . 911 917 + TspRI CASTGNN 917 908 . . 920 923 + TspEI AATT 919 923 . . 927 930 + TspEI AATT 926 930 . . 937 940 + SetI ASST 940 936 . . 934 938 - BsmAI GTCTC 928 932 . . 934 939 - Eco31I GGTCTC 928 932 . . 946 951 + Hpy166II GTNNAC 948 948 . . 953 957 + DdeI CTNAG 953 956 . . 961 966 + Hpy166II GTNNAC 963 963 . . 961 966 + HpaI GTTAAC 963 963 . . 961 966 + HindII GTYRAC 963 963 . . 962 965 + MseI TTAA 962 964 . . 970 975 + VspI ATTAAT 971 973 . . 971 974 + MseI TTAA 971 973 . . 973 976 + TspEI AATT 972 976 . . 980 984 + Hin4II* CCTTC 990 989 . . 995 999 + Hpy188I TCNGA 997 996 . . 999 1002 + TspEI AATT 998 1002 . . 1005 1008 + CviJI RGCY 1006 1006 . . 1009 1012 + MseI TTAA 1009 1011 . . 1017 1020 + CviJI RGCY 1018 1018 . . 1018 1022 + TauI GCSGC 1021 1018 . . 1018 1022 + BlsI GCNGC 1020 1019 . . 1018 1022 + BisI GCNGC 1019 1020 . . 1019 1022 + AciI CCGC 1019 1021 . . 1028 1031 + SetI ASST 1031 1027 . . 1031 1034 + MseI TTAA 1031 1033 . . #--------------------------------------- # # # AciI: # [CCGC] # 25 156 364 499 # # AclI: # [AACGTT] # 100 944 # # AluI: # [AGCT] # 18 85 103 193 271 374 # # AlwNI: # [CAGNNNCTG] # 184 860 # # ApoI: # [RAATTY] # 229 815 # # AsuII: # [TTCGAA] # 368 676 # # AvaI: # [CYCGRG] # 276 768 # # BbvI: # [GCAGC] # 197 847 # # Bce83I*: # [CTTGAG] # 190 854 # # BfiI: # [ACTGGG] # 135 909 # # BinI*: # [GGATC] # 243 801 # # BisI: # [GCNGC] # 3 25 155 861 # # BlsI: # [GCNGC] # 3 24 155 862 # # BmeT110I: # [CYCGRG] # 277 767 # # BsaBI: # [GATNNNNATC] # 326 718 # # BseYI: # [CCCAGC] # 28 332 684 # # BsiI*: # [CACGAG] # 442 602 # # BsiYI*: # [CCNNNNNNNGG] # 1 144 242 657 # # BsmAI: # [GTCTC] # 116 928 # # Bsp1407I: # [TGTACA] # 94 950 # # BsrDI: # [GCAATG] # 189 359 496 # # BsrI: # [ACTGG] # 21 140 186 697 # # BstAPI: # [GCANNNNNTGC] # 184 860 # # BstKTI: # [GATC] # 13 49 135 140 182 235 # 290 # # BtsI: # [GCAGTG] # 127 396 521 # # Cac8I: # [GCNNGC] # 136 908 # # ClaI: # [ATCGAT] # 307 737 # # Csp6I: # [GTAC] # 95 445 504 # # CspCI: # [CAANNNNNGTGG] # 35 464 545 # # CviAII: # [CATG] # 290 331 423 # # CviJI: # [RGCY] # 10 12 18 26 35 43 # 44 46 50 51 51 61 # 85 93 136 140 143 # # CviRI*: # [TGCA] # 12 196 345 491 # # DdeI: # [CTNAG] # 91 109 387 457 # # DpnI: # [GATC] # 13 49 134 140 182 236 # 290 # # DraIII: # [CACNNNGTG] # 132 912 # # DsaI*: # [CCRYGG] # 424 620 # # Eco31I: # [GGTCTC] # 116 928 # # EcoP15I: # [CAGCAG] # 179 865 # # FalI: # [AAGNNNNNCTT] # 0 0 32 229 783 # # FatI: # [CATG] # 290 330 424 # # GlaI: # [GCGC] # 139 410 495 # # GsaI: # [CCCAGC] # 32 328 684 # # HgaI: # [GACGC] # 178 866 # # HgiJII*: # [GRGCYC] # 416 628 # # HhaI: # [GCGC] # 138 411 495 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 214 361 405 # # Hin4II*: # [CCTTC] # 54 483 507 # # Hin6I: # [GCGC] # 140 409 495 # # HindII: # [GTYRAC] # 81 963 # # HinfI: # [GANTC] # 12 31 254 345 402 # # HpaI: # [GTTAAC] # 81 963 # # HphI: # [GGTGA] # 414 630 # # Hpy166II: # [GTNNAC] # 15 81 948 # # Hpy178III*: # [TCNNGA] # 2 22 173 254 593 # # Hpy188I: # [TCNGA] # 47 149 207 641 # # Ksp632I*: # [CTCTTC] # 479 565 # # MaeI: # [CTAG] # 33 80 236 274 421 # # MaeII: # [ACGT] # 100 152 792 # # MaeIII: # [GTNAC] # 202 211 279 352 # # MboI: # [GATC] # 13 49 132 140 182 238 # 290 # # MboII: # [GAAGA] # 165 241 307 331 # # MmeI: # [TCCRAC] # 404 640 # # MnlI: # [CCTC] # 18 69 273 684 # # MseI: # [TTAA] # 9 13 22 38 39 54 # 81 88 121 132 140 307 # # MstI*: # [TGCGCA] # 410 634 # # MwoI: # [GCNNNNNNNGC] # 184 860 # # NcoI: # [CCATGG] # 424 620 # # NlaIII: # [CATG] # 290 334 420 # # RsaI: # [GTAC] # 96 444 504 # # SduI: # [GDGCHC] # 416 628 # # SecI*: # [CCNNGG] # 424 620 # # SetI: # [ASST] # 13 20 20 26 57 79 # 85 91 114 132 165 242 # # SfaNI: # [GCATC] # 324 720 # # SmlI: # [CTYRAG] # 175 276 593 # # SspI: # [AATATT] # 504 540 # # StyI: # [CCWWGG] # 424 620 # # TaiI: # [ACGT] # 103 152 789 # # TaqI: # [TCGA] # 38 61 116 161 269 399 # # TatI: # [WGTACW] # 94 950 # # TauI: # [GCSGC] # 23 155 866 # # TfiI: # [GAWTC] # 12 31 254 345 402 # # TseI: # [GCWGC] # 184 860 # # TsoI: # [TARCCA] # 16 267 366 395 # # Tsp45I: # [GTSAC] # 211 279 554 # # Tsp4CI*: # [ACNGT] # 24 97 147 154 193 209 # 220 # # TspDTI: # [ATGAA] # 298 345 401 # # TspEI: # [AATT] # 7 26 27 46 46 63 # 73 108 136 156 174 182 # # TspRI: # [CASTGNN] # 127 147 207 242 321 # # VspI: # [ATTAAT] # 73 213 223 535 # # XcmI: # [CCANNNNNNNNNTGG] # 390 654 # # XhoI: # [CTCGAG] # 276 768 # # XmnI: # [GAANNNNTTC] # 362 682 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1044 # Reported_sequences: 1 # Reported_hitcount: 230 #---------------------------------------