Restriction Fragments for IML3/YBR107C



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The currently selected gene/sequence is :

IML3/YBR107C on chromosome II from coordinates 454530 to 453793.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.2279.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 26 30 + TfiI GAWTC 26 29 . . 26 30 + HinfI GANTC 26 29 . . 32 37 + Cac8I GCNNGC 34 34 . . 44 50 + TspRI CASTGNN 50 41 . . 45 49 + BsrI ACTGG 50 48 . . 73 78 + MfeI CAATTG 73 77 . . 74 77 + TspEI AATT 73 77 . . 83 86 + SetI ASST 86 82 . . 84 88 + BssKI CCNGG 83 88 . . 84 88 + BseBI CCWGG 85 86 . . 84 88 + ScrFI CCNGG 85 86 . . 84 88 + EcoRII CCWGG 83 88 . . 114 117 + TaqI TCGA 114 116 . . 118 121 + Csp6I GTAC 118 120 . . 118 121 + RsaI GTAC 119 119 . . 123 129 + TspRI CASTGNN 129 120 . . 122 126 - BsrI ACTGG 122 120 . . 121 126 - BfiI ACTGGG 116 115 . . 127 130 + DpnI GATC 128 128 . . 127 130 + BstKTI GATC 129 127 . . 127 130 + MboI GATC 126 130 . . 129 134 + Hpy178III* TCNNGA 130 132 . . 130 140 + BsiYI* CCNNNNNNNGG 136 133 . . 127 131 - BinI* GGATC 121 122 . . 133 137 - BccI CCATC 127 128 . . 139 144 + KpnI GGTACC 143 139 . . 139 144 + NlaIV GGNNCC 141 141 . . 139 144 + HgiCI* GGYRCC 139 143 . . 139 144 + Acc65I GGTACC 139 143 . . 140 143 + Csp6I GTAC 140 142 . . 140 143 + RsaI GTAC 141 141 . . 143 146 + AciI CCGC 143 145 . . 145 149 + TauI GCSGC 148 145 . . 145 149 + BlsI GCNGC 147 146 . . 145 149 + BisI GCNGC 146 147 . . 146 151 + NspBII* CMGCKG 148 148 . . 146 149 + AciI CCGC 146 148 . . 164 168 + MaeIII GTNAC 163 168 . . 161 165 - TspGWI ACGGA 151 149 . . 175 178 + SetI ASST 178 174 . . 174 177 - MnlI CCTC 167 166 . . 177 182 - MmeI TCCRAC 158 156 . . 190 193 + TspEI AATT 189 193 . . 201 206 + SduI GDGCHC 205 201 . . 201 206 + BseSI GKGCMC 205 201 . . 199 202 - MnlI CCTC 192 191 . . 212 216 + DdeI CTNAG 212 215 . . 217 221 - TspDTI ATGAA 207 205 . . 245 248 + SetI ASST 248 244 . . 246 251 + NlaIV GGNNCC 248 248 . . 246 251 + HgiCI* GGYRCC 246 250 . . 244 247 - MnlI CCTC 237 236 . . 271 275 + BslFI GGGAC 285 289 . . 269 272 - AciI CCGC 269 271 . . 269 273 - FauI CCCGC 262 264 . . 282 285 + AciI CCGC 282 284 . . 284 289 + BsrDI GCAATG 291 289 . . 287 291 + TspDTI ATGAA 302 300 . . 299 302 + CviRI* TGCA 300 300 . . 300 305 + BtsI GCAGTG 307 305 . . 301 307 + TspRI CASTGNN 307 298 . . 301 309 + AlwNI CAGNNNCTG 306 303 . . 305 308 + CviJI RGCY 306 306 . . 307 312 + Eco57MI CTGRAG 328 326 . . 307 312 + Eco57I CTGAAG 328 326 . . 309 321 + BarI GAAGNNNNNNTAC 301 296 333 328 309 313 - Hin4II* CCTTC 303 302 . . 318 321 + Csp6I GTAC 318 320 . . 318 321 + RsaI GTAC 319 319 . . 320 324 + Tsp4CI* ACNGT 322 321 . . 334 339 + CfrI YGGCCR 334 338 . . 335 338 + CviJI RGCY 336 336 . . 335 338 + HaeIII GGCC 336 336 . . 343 348 + ApoI RAATTY 343 347 . . 344 347 + TspEI AATT 343 347 . . 354 358 + MboII GAAGA 366 365 . . 354 359 + BbvII* GAAGAC 361 365 . . 360 363 + FatI CATG 359 363 . . 360 363 + NlaIII CATG 363 359 . . 360 363 + CviAII CATG 360 362 . . 372 375 + SetI ASST 375 371 . . 372 375 + CviJI RGCY 373 373 . . 372 375 + AluI AGCT 373 373 . . 376 380 + HgaI GACGC 385 390 . . 376 381 + AcyI GRCGYC 377 379 . . 379 384 + Cac8I GCNNGC 381 381 . . 388 393 + Hpy166II GTNNAC 390 390 . . 388 393 + SduI GDGCHC 392 388 . . 388 393 + BseSI GKGCMC 392 388 . . 388 393 + ApaLI GTGCAC 388 392 . . 388 393 + HgiAI* GWGCWC 392 388 . . 389 392 + CviRI* TGCA 390 390 . . 391 396 + BsaAI YACGTR 393 393 . . 392 395 + TaiI ACGT 395 391 . . 392 395 + SetI ASST 395 391 . . 392 395 + MaeII ACGT 392 394 . . 394 398 + MaeIII GTNAC 393 398 . . 410 413 + TaqI TCGA 410 412 . . 417 420 + TspEI AATT 416 420 . . 421 424 + SetI ASST 424 420 . . 421 427 + SexAI ACCWGGT 421 426 . . 422 426 + BssKI CCNGG 421 426 . . 422 426 + BseBI CCWGG 423 424 . . 422 426 + ScrFI CCNGG 423 424 . . 422 426 + EcoRII CCWGG 421 426 . . 430 433 + SetI ASST 433 429 . . 430 433 + CviJI RGCY 431 431 . . 430 433 + AluI AGCT 431 431 . . 474 478 + TauI GCSGC 477 474 . . 474 478 + BlsI GCNGC 476 475 . . 474 478 + BisI GCNGC 475 476 . . 474 477 - AciI CCGC 474 476 . . 479 482 + TspEI AATT 478 482 . . 505 508 + SetI ASST 508 504 . . 505 508 + CviJI RGCY 506 506 . . 505 508 + AluI AGCT 506 506 . . 511 514 - MnlI CCTC 504 503 . . 519 522 + CviJI RGCY 520 520 . . 526 529 + CviJI RGCY 527 527 . . 525 530 - TsoI TARCCA 515 513 . . 542 545 + CviRI* TGCA 543 543 . . 558 561 + CviRI* TGCA 559 559 . . 560 565 + MfeI CAATTG 560 564 . . 556 560 - SfaNI GCATC 546 550 . . 561 564 + TspEI AATT 560 564 . . 565 568 + Csp6I GTAC 565 567 . . 565 568 + RsaI GTAC 566 566 . . 576 581 + PsiI TTATAA 578 578 . . 581 585 + TspDTI ATGAA 596 594 . . 590 594 + FokI GGATG 603 607 . . 590 594 + BseGI GGATG 596 594 . . 591 595 - BccI CCATC 585 586 . . 595 598 - AciI CCGC 595 597 . . 609 613 + Tsp4CI* ACNGT 611 610 . . 612 617 + Hpy166II GTNNAC 614 614 . . 612 617 + HindII GTYRAC 614 614 . . 614 625 + BdaI TGANNNNNNTCA 603 601 637 635 615 624 + PshAI GACNNNNGTC 619 619 . . 619 623 - TspGWI ACGGA 609 607 . . 614 625 - BdaI TGANNNNNNTCA 637 635 603 601 634 639 + SduI GDGCHC 638 634 . . 634 639 + BseSI GKGCMC 638 634 . . 655 659 + TfiI GAWTC 655 658 . . 655 659 + HinfI GANTC 655 658 . . 664 668 + Tsp45I GTSAC 663 668 . . 664 668 + MaeIII GTNAC 663 668 . . 680 684 + BsmAI GTCTC 685 689 . . 682 686 + DdeI CTNAG 682 685 . . 682 686 + BspCNI CTCAG 695 693 . . 682 686 + BseMII CTCAG 696 694 . . 683 688 + Hpy178III* TCNNGA 684 686 . . 697 700 + MaeI CTAG 697 699 . . 705 710 + TatI WGTACW 705 709 . . 706 709 + Csp6I GTAC 706 708 . . 706 709 + RsaI GTAC 707 707 . . 714 719 + ClaI ATCGAT 715 717 . . 715 718 + TaqI TCGA 715 717 . . 717 721 + TfiI GAWTC 717 720 . . 717 721 + HinfI GANTC 717 720 . . 723 728 + Hpy166II GTNNAC 725 725 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 139 599 # # AciI: # [CCGC] # 3 13 121 123 143 143 # 192 # # AcyI: # [GRCGYC] # 361 377 # # AluI: # [AGCT] # 58 75 232 373 # # AlwNI: # [CAGNNNCTG] # 306 432 # # ApaLI: # [GTGCAC] # 350 388 # # ApoI: # [RAATTY] # 343 395 # # BarI: # [GAAGNNNNNNTAC] # 32 301 405 # # BbvII*: # [GAAGAC] # 361 377 # # BccI: # [CCATC] # 127 153 458 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 101 603 # # BfiI: # [ACTGGG] # 116 622 # # BinI*: # [GGATC] # 121 617 # # BisI: # [GCNGC] # 146 263 329 # # BlsI: # [GCNGC] # 147 262 329 # # BsaAI: # [YACGTR] # 345 393 # # BseBI: # [CCWGG] # 85 315 338 # # BseGI: # [GGATG] # 142 596 # # BseMII: # [CTCAG] # 42 696 # # BseSI: # [GKGCMC] # 100 187 205 246 # # BsiYI*: # [CCNNNNNNNGG] # 136 602 # # BslFI: # [GGGAC] # 285 453 # # BsmAI: # [GTCTC] # 53 685 # # BspCNI: # [CTCAG] # 43 695 # # BsrDI: # [GCAATG] # 291 447 # # BsrI: # [ACTGG] # 50 72 616 # # BssKI: # [CCNGG] # 83 317 338 # # BstKTI: # [GATC] # 129 609 # # BtsI: # [GCAGTG] # 307 431 # # Cac8I: # [GCNNGC] # 34 347 357 # # CfrI: # [YGGCCR] # 334 404 # # ClaI: # [ATCGAT] # 23 715 # # Csp6I: # [GTAC] # 22 32 118 141 178 247 # # CviAII: # [CATG] # 360 378 # # CviJI: # [RGCY] # 7 14 30 37 58 75 # 211 306 # # CviRI*: # [TGCA] # 16 90 153 179 300 # # DdeI: # [CTNAG] # 56 212 470 # # DpnI: # [GATC] # 128 610 # # Eco57I: # [CTGAAG] # 328 410 # # Eco57MI: # [CTGRAG] # 328 410 # # EcoRII: # [CCWGG] # 83 317 338 # # FatI: # [CATG] # 359 379 # # FauI: # [CCCGC] # 262 476 # # FokI: # [GGATG] # 135 603 # # HaeIII: # [GGCC] # 336 402 # # HgaI: # [GACGC] # 353 385 # # HgiAI*: # [GWGCWC] # 346 392 # # HgiCI*: # [GGYRCC] # 107 139 492 # # Hin4II*: # [CCTTC] # 303 435 # # HindII: # [GTYRAC] # 124 614 # # HinfI: # [GANTC] # 21 26 62 629 # # Hpy166II: # [GTNNAC] # 13 111 224 390 # # Hpy178III*: # [TCNNGA] # 54 130 554 # # KpnI: # [GGTACC] # 143 595 # # MaeI: # [CTAG] # 41 697 # # MaeII: # [ACGT] # 346 392 # # MaeIII: # [GTNAC] # 75 163 230 270 # # MboI: # [GATC] # 126 612 # # MboII: # [GAAGA] # 366 372 # # MfeI: # [CAATTG] # 73 178 487 # # MmeI: # [TCCRAC] # 158 580 # # MnlI: # [CCTC] # 25 45 167 234 267 # # NlaIII: # [CATG] # 363 375 # # NlaIV: # [GGNNCC] # 107 141 490 # # NspBII*: # [CMGCKG] # 148 590 # # PshAI: # [GACNNNNGTC] # 119 619 # # PsiI: # [TTATAA] # 160 578 # # RsaI: # [GTAC] # 22 31 119 141 178 247 # # ScrFI: # [CCNGG] # 85 315 338 # # SduI: # [GDGCHC] # 100 187 205 246 # # SetI: # [ASST] # 9 20 29 70 75 86 # 92 127 230 # # SexAI: # [ACCWGGT] # 317 421 # # SfaNI: # [GCATC] # 192 546 # # TaiI: # [ACGT] # 343 395 # # TaqI: # [TCGA] # 23 114 296 305 # # TatI: # [WGTACW] # 33 705 # # TauI: # [GCSGC] # 148 261 329 # # TfiI: # [GAWTC] # 21 26 62 629 # # TsoI: # [TARCCA] # 223 515 # # Tsp45I: # [GTSAC] # 75 663 # # Tsp4CI*: # [ACNGT] # 127 289 322 # # TspDTI: # [ATGAA] # 95 142 207 294 # # TspEI: # [AATT] # 62 73 73 82 116 154 # 178 # # TspGWI: # [ACGGA] # 129 151 458 # # TspRI: # [CASTGNN] # 50 79 178 431 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 738 # Reported_sequences: 1 # Reported_hitcount: 160 #---------------------------------------