Restriction Fragments for MIS1/YBR084W



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The currently selected gene/sequence is :

MIS1/YBR084W on chromosome II from coordinates 411054 to 413981.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.1070.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 10 + TaqI TCGA 7 9 . . 7 12 + Hpy178III* TCNNGA 8 10 . . 9 13 - BsmAI GTCTC 3 7 . . 26 30 + MaeIII GTNAC 25 30 . . 29 36 + PspXI VCTCGAGB 30 34 . . 30 35 + SmlI CTYRAG 30 34 . . 30 35 + XhoI CTCGAG 30 34 . . 30 35 + BmeT110I CYCGRG 31 33 . . 30 35 + AvaI CYCGRG 30 34 . . 31 34 + TaqI TCGA 31 33 . . 33 36 - MnlI CCTC 26 25 . . 41 46 + MmeI TCCRAC 66 64 . . 48 51 + CviJI RGCY 49 49 . . 48 51 + HaeIII GGCC 49 49 . . 57 60 + Hin6I GCGC 57 59 . . 57 60 + GlaI GCGC 58 58 . . 53 57 - BccI CCATC 47 48 . . 57 60 + HhaI GCGC 59 57 . . 59 69 + MwoI GCNNNNNNNGC 65 62 . . 66 69 + AciI CCGC 66 68 . . 70 73 + MseI TTAA 70 72 . . 81 91 + MwoI GCNNNNNNNGC 87 84 . . 85 89 + Tsp4CI* ACNGT 87 86 . . 89 92 + CviRI* TGCA 90 90 . . 90 95 + NspI RCATGY 94 90 . . 90 95 + Cac8I GCNNGC 92 92 . . 90 95 + SphI GCATGC 94 90 . . 91 94 + FatI CATG 90 94 . . 91 94 + NlaIII CATG 94 90 . . 91 94 + CviAII CATG 91 93 . . 105 119 + XcmI CCANNNNNNNNNTGG 112 111 . . 141 144 + TaqI TCGA 141 143 . . 141 146 + Hpy178III* TCNNGA 142 144 . . 150 153 + CviJI RGCY 151 151 . . 168 171 + CviJI RGCY 169 169 . . 173 176 + MseI TTAA 173 175 . . 175 180 + HindIII AAGCTT 175 179 . . 176 179 + SetI ASST 179 175 . . 176 179 + CviJI RGCY 177 177 . . 176 179 + AluI AGCT 177 177 . . 178 183 + SmlI CTYRAG 178 182 . . 178 183 + AflII CTTAAG 178 182 . . 179 182 + MseI TTAA 179 181 . . 183 187 + SfaNI GCATC 192 196 . . 184 188 - FokI GGATG 170 174 . . 184 188 - BseGI GGATG 183 181 . . 190 193 + TspEI AATT 189 193 . . 196 206 + FalI AAGNNNNNCTT 187 182 219 214 197 200 + CviJI RGCY 198 198 . . 202 205 + SetI ASST 205 201 . . 196 206 - FalI AAGNNNNNCTT 219 214 187 182 223 228 + NlaIV GGNNCC 225 225 . . 224 228 + BmgT120I GGNCC 225 226 . . 224 228 + AsuI* GGNCC 224 227 . . 225 228 + CviJI RGCY 226 226 . . 225 228 + HaeIII GGCC 226 226 . . 235 239 + HinfI GANTC 235 238 . . 235 239 - PleI GAGTC 229 230 . . 235 239 - MlyI GAGTC 229 229 . . 243 248 + SnaBI TACGTA 245 245 . . 243 248 + BsaAI YACGTR 245 245 . . 244 247 + TaiI ACGT 247 243 . . 244 247 + SetI ASST 247 243 . . 244 247 + MaeII ACGT 244 246 . . 245 250 + SplI* CGTACG 245 249 . . 246 249 + Csp6I GTAC 246 248 . . 246 249 + RsaI GTAC 247 247 . . 247 252 + BsaAI YACGTR 249 249 . . 248 251 + TaiI ACGT 251 247 . . 248 251 + SetI ASST 251 247 . . 248 251 + MaeII ACGT 248 250 . . 254 258 + FokI GGATG 267 271 . . 254 258 + BseGI GGATG 260 258 . . 252 255 - MnlI CCTC 245 244 . . 256 260 + TspDTI ATGAA 271 269 . . 260 263 + TspEI AATT 259 263 . . 266 271 + HindIII AAGCTT 266 270 . . 267 270 + SetI ASST 270 266 . . 267 270 + CviJI RGCY 268 268 . . 267 270 + AluI AGCT 268 268 . . 281 286 + BsrDI GCAATG 288 286 . . 286 291 + Hpy166II GTNNAC 288 288 . . 286 291 + AccI GTMKAC 287 289 . . 290 294 + Tsp4CI* ACNGT 292 291 . . 306 310 + MaeIII GTNAC 305 310 . . 311 316 + EcoP15I CAGCAG 341 343 . . 321 327 + TspRI CASTGNN 327 318 . . 323 328 + Eco57MI CTGRAG 344 342 . . 323 328 + Eco57I CTGAAG 344 342 . . 326 336 + FalI AAGNNNNNCTT 317 312 349 344 332 335 + SetI ASST 335 331 . . 332 335 + CviJI RGCY 333 333 . . 332 335 + AluI AGCT 333 333 . . 326 336 - FalI AAGNNNNNCTT 349 344 317 312 339 343 + MboII GAAGA 351 350 . . 345 348 + TspEI AATT 344 348 . . 350 354 + Tsp45I GTSAC 349 354 . . 350 354 + MaeIII GTNAC 349 354 . . 361 371 + Hin4I GAYNNNNNVTC 352 347 384 379 367 371 + HinfI GANTC 367 370 . . 367 371 - PleI GAGTC 361 362 . . 367 371 - MlyI GAGTC 361 361 . . 375 380 + DsaI* CCRYGG 375 379 . . 375 380 + StyI CCWWGG 375 379 . . 375 380 + NcoI CCATGG 375 379 . . 375 380 + SecI* CCNNGG 375 379 . . 376 379 + FatI CATG 375 379 . . 376 379 + NlaIII CATG 379 375 . . 376 379 + CviAII CATG 376 378 . . 387 390 + TspEI AATT 386 390 . . 396 399 + SetI ASST 399 395 . . 397 400 + MnlI CCTC 407 406 . . 403 406 + AciI CCGC 403 405 . . 404 407 + FnuDII* CGCG 405 405 . . 407 411 + Tsp45I GTSAC 406 411 . . 407 411 + MaeIII GTNAC 406 411 . . 409 418 + MslI CAYNNNNRTG 413 413 . . 405 409 - HgaI GACGC 394 399 . . 414 418 + FokI GGATG 427 431 . . 414 418 + BseGI GGATG 420 418 . . 416 420 + TspDTI ATGAA 431 429 . . 431 442 + CspCI CAANNNNNGTGG 419 417 454 452 439 444 + Hpy166II GTNNAC 441 441 . . 445 450 + AhaIII* TTTAAA 447 447 . . 446 449 + MseI TTAA 446 448 . . 455 458 + TaiI ACGT 458 454 . . 455 458 + SetI ASST 458 454 . . 455 458 + MaeII ACGT 455 457 . . 464 469 + NlaIV GGNNCC 466 466 . . 460 464 - BccI CCATC 454 455 . . 484 488 + HphI GGTGA 496 495 . . 488 491 + TspEI AATT 487 491 . . 492 495 + CviJI RGCY 493 493 . . 491 496 - TsoI TARCCA 481 479 . . 503 506 - MnlI CCTC 496 495 . . 517 521 - TspDTI ATGAA 507 505 . . 524 527 + FatI CATG 523 527 . . 524 527 + NlaIII CATG 527 523 . . 524 527 + CviAII CATG 524 526 . . 526 531 + TatI WGTACW 526 530 . . 527 530 + Csp6I GTAC 527 529 . . 527 530 + RsaI GTAC 528 528 . . 541 544 + CviRI* TGCA 542 542 . . 543 546 + FatI CATG 542 546 . . 543 546 + NlaIII CATG 546 542 . . 543 546 + CviAII CATG 543 545 . . 544 548 + TspDTI ATGAA 559 557 . . 548 551 + TspEI AATT 547 551 . . 555 566 + BdaI TGANNNNNNTCA 544 542 578 576 556 560 + MboII GAAGA 568 567 . . 561 564 + SetI ASST 564 560 . . 561 564 + CviJI RGCY 562 562 . . 561 564 + AluI AGCT 562 562 . . 565 568 + FatI CATG 564 568 . . 565 568 + NlaIII CATG 568 564 . . 565 568 + CviAII CATG 565 567 . . 555 566 - BdaI TGANNNNNNTCA 578 576 544 542 569 572 + MseI TTAA 569 571 . . 577 581 - BccI CCATC 571 572 . . 591 595 + Hpy99I CGWCG 595 590 . . 589 593 - BceAI ACGGC 574 576 . . 605 615 + Hin4I GAYNNNNNVTC 596 591 628 623 605 608 + DpnI GATC 606 606 . . 605 608 + BstKTI GATC 607 605 . . 605 608 + MboI GATC 604 608 . . 615 619 + Hpy99I CGWCG 619 614 . . 605 615 - Hin4I GAYNNNNNVTC 628 623 596 591 617 621 + Hpy188I TCNGA 619 618 . . 616 621 - MmeI TCCRAC 597 595 . . 626 631 + MfeI CAATTG 626 630 . . 627 630 + TspEI AATT 626 630 . . 651 656 + BsmI GAATGC 657 655 . . 657 663 + TspRI CASTGNN 663 654 . . 658 662 + Tsp4CI* ACNGT 660 659 . . 661 665 + MaeIII GTNAC 660 665 . . 664 668 + Tsp4CI* ACNGT 666 665 . . 693 696 + CviRI* TGCA 694 694 . . 690 695 - BsrDI GCAATG 689 687 . . 702 707 + Eco31I GGTCTC 708 712 . . 703 707 + BsmAI GTCTC 708 712 . . 711 714 + SetI ASST 714 710 . . 711 714 + CviJI RGCY 712 712 . . 711 714 + AluI AGCT 712 712 . . 727 731 + BlsI GCNGC 729 728 . . 727 731 + BisI GCNGC 728 729 . . 727 731 + BbvI GCAGC 739 743 . . 727 731 + TseI GCWGC 727 730 . . 729 732 + SetI ASST 732 728 . . 729 732 + CviJI RGCY 730 730 . . 729 732 + AluI AGCT 730 730 . . 730 735 + Cac8I GCNNGC 732 732 . . 734 737 - AciI CCGC 734 736 . . 742 745 + MnlI CCTC 752 751 . . 749 752 + TaiI ACGT 752 748 . . 749 752 + SetI ASST 752 748 . . 749 752 + MaeII ACGT 749 751 . . 752 755 + MseI TTAA 752 754 . . 757 761 + Hpy188I TCNGA 759 758 . . 761 765 + BsrI ACTGG 766 764 . . 767 770 + MseI TTAA 767 769 . . 775 780 + NarI GGCGCC 776 778 . . 775 780 + KasI GGCGCC 775 779 . . 775 780 + DinI GGCGCC 777 777 . . 775 780 + HaeII RGCGCY 779 775 . . 775 780 + NlaIV GGNNCC 777 777 . . 775 780 + AcyI GRCGYC 776 778 . . 775 780 + HgiCI* GGYRCC 775 779 . . 772 776 - Hin4II* CCTTC 766 765 . . 776 779 + Hin6I GCGC 776 778 . . 776 779 + GlaI GCGC 777 777 . . 776 779 + HhaI GCGC 778 776 . . 779 784 + DsaI* CCRYGG 779 783 . . 779 784 + SecI* CCNNGG 779 783 . . 778 782 - BceAI ACGGC 763 765 . . 782 787 - TsoI TARCCA 772 770 . . 795 798 + SetI ASST 798 794 . . 805 810 + SnaBI TACGTA 807 807 . . 805 810 + BsaAI YACGTR 807 807 . . 806 809 + TaiI ACGT 809 805 . . 806 809 + SetI ASST 809 805 . . 806 809 + MaeII ACGT 806 808 . . 808 811 + Csp6I GTAC 808 810 . . 808 811 + RsaI GTAC 809 809 . . 810 813 + SetI ASST 813 809 . . 814 819 + EcoRV GATATC 816 816 . . 839 842 + TspEI AATT 838 842 . . 847 851 + HphI GGTGA 859 858 . . 862 866 + TfiI GAWTC 862 865 . . 862 866 + HinfI GANTC 862 865 . . 895 905 + BsiYI* CCNNNNNNNGG 901 898 . . 901 905 + BssKI CCNGG 900 905 . . 901 905 + BseBI CCWGG 902 903 . . 901 905 + ScrFI CCNGG 902 903 . . 901 905 + EcoRII CCWGG 900 905 . . 912 917 + NlaIV GGNNCC 914 914 . . 913 917 + BmgT120I GGNCC 914 915 . . 913 917 + AvaII GGWCC 913 916 . . 913 917 + AsuI* GGNCC 913 916 . . 922 926 + Tsp4CI* ACNGT 924 923 . . 945 950 + TatI WGTACW 945 949 . . 946 949 + Csp6I GTAC 946 948 . . 946 949 + RsaI GTAC 947 947 . . 954 957 + SetI ASST 957 953 . . 954 957 + CviJI RGCY 955 955 . . 954 957 + AluI AGCT 955 955 . . 955 959 + BlsI GCNGC 957 956 . . 955 959 + BisI GCNGC 956 957 . . 955 959 + TseI GCWGC 955 958 . . 955 959 - BbvI GCAGC 942 946 . . 968 971 + TspEI AATT 967 971 . . 976 980 + TfiI GAWTC 976 979 . . 976 980 + HinfI GANTC 976 979 . . 979 983 + Hpy188I TCNGA 981 980 . . 982 991 + XmnI GAANNNNTTC 986 986 . . 985 990 + HindIII AAGCTT 985 989 . . 986 989 + SetI ASST 989 985 . . 986 989 + CviJI RGCY 987 987 . . 986 989 + AluI AGCT 987 987 . . 991 995 - BsrI ACTGG 991 989 . . 1002 1005 + SetI ASST 1005 1001 . . 1003 1006 + MnlI CCTC 1013 1012 . . 1004 1009 + Ksp632I* CTCTTC 1010 1013 . . 1005 1009 - MboII GAAGA 997 996 . . 1017 1021 + DdeI CTNAG 1017 1020 . . 1028 1034 + TspRI CASTGNN 1034 1025 . . 1027 1031 - BsrI ACTGG 1027 1025 . . 1033 1037 + Hin4II* CCTTC 1043 1042 . . 1036 1040 + Hpy188I TCNGA 1038 1037 . . 1034 1039 - Eco57MI CTGRAG 1019 1017 . . 1034 1039 - Eco57I CTGAAG 1019 1017 . . 1051 1056 + Hpy178III* TCNNGA 1052 1054 . . 1055 1060 + SacI GAGCTC 1059 1055 . . 1055 1060 + Ecl136II GAGCTC 1057 1057 . . 1055 1060 + SduI GDGCHC 1059 1055 . . 1055 1060 + HgiJII* GRGCYC 1059 1055 . . 1055 1060 + HgiAI* GWGCWC 1059 1055 . . 1056 1059 + SetI ASST 1059 1055 . . 1056 1059 + CviJI RGCY 1057 1057 . . 1056 1059 + AluI AGCT 1057 1057 . . 1058 1062 + DdeI CTNAG 1058 1061 . . 1058 1062 + BspCNI CTCAG 1071 1069 . . 1058 1062 + BseMII CTCAG 1072 1070 . . 1059 1063 + Hpy188I TCNGA 1061 1060 . . 1062 1066 + PleI GAGTC 1070 1071 . . 1062 1066 + MlyI GAGTC 1071 1071 . . 1062 1066 + HinfI GANTC 1062 1065 . . 1066 1072 + Bpu10I CCTNAGC 1067 1070 . . 1067 1071 + DdeI CTNAG 1067 1070 . . 1071 1081 + MwoI GCNNNNNNNGC 1077 1074 . . 1087 1092 + NmeAIII GCCGAG 1113 1111 . . 1088 1093 + SecI* CCNNGG 1088 1092 . . 1090 1095 + BseRI GAGGAG 1105 1103 . . 1090 1093 - MnlI CCTC 1083 1082 . . 1100 1104 + TfiI GAWTC 1100 1103 . . 1100 1104 + HinfI GANTC 1100 1103 . . 1110 1115 + Hpy178III* TCNNGA 1111 1113 . . 1115 1118 + TspEI AATT 1114 1118 . . 1121 1124 + TspEI AATT 1120 1124 . . 1127 1131 + BceAI ACGGC 1143 1145 . . 1128 1133 + CfrI YGGCCR 1128 1132 . . 1129 1132 + CviJI RGCY 1130 1130 . . 1129 1132 + HaeIII GGCC 1130 1130 . . 1131 1141 + BsiYI* CCNNNNNNNGG 1137 1134 . . 1145 1150 + ApoI RAATTY 1145 1149 . . 1146 1149 + TspEI AATT 1145 1149 . . 1156 1161 + SspI AATATT 1158 1158 . . 1162 1167 + AhaIII* TTTAAA 1164 1164 . . 1163 1166 + MseI TTAA 1163 1165 . . 1169 1178 + BsaBI GATNNNNATC 1173 1173 . . 1174 1178 + TfiI GAWTC 1174 1177 . . 1174 1178 + HinfI GANTC 1174 1177 . . 1177 1182 + XbaI TCTAGA 1177 1181 . . 1177 1182 + Hpy178III* TCNNGA 1178 1180 . . 1178 1181 + MaeI CTAG 1178 1180 . . 1187 1191 + Tsp4CI* ACNGT 1189 1188 . . 1194 1197 + Csp6I GTAC 1194 1196 . . 1194 1197 + RsaI GTAC 1195 1195 . . 1196 1199 + TaiI ACGT 1199 1195 . . 1196 1199 + SetI ASST 1199 1195 . . 1196 1199 + MaeII ACGT 1196 1198 . . 1206 1209 + CviRI* TGCA 1207 1207 . . 1209 1212 + SetI ASST 1212 1208 . . 1207 1212 - BspMI ACCTGC 1198 1202 . . 1216 1226 + BsaXI ACNNNNNCTCC 1206 1203 1236 1233 1218 1223 + MmeI TCCRAC 1243 1241 . . 1218 1222 + Hpy188I TCNGA 1220 1219 . . 1221 1225 + HinfI GANTC 1221 1224 . . 1221 1225 - PleI GAGTC 1215 1216 . . 1221 1225 - MlyI GAGTC 1215 1215 . . 1231 1235 + HphI GGTGA 1243 1242 . . 1229 1234 - TaqII CACCCA 1219 1217 . . 1236 1239 + SetI ASST 1239 1235 . . 1234 1238 - Hin4II* CCTTC 1228 1227 . . 1244 1258 + XcmI CCANNNNNNNNNTGG 1251 1250 . . 1264 1269 + BsgI GTGCAG 1285 1283 . . 1265 1268 + CviRI* TGCA 1266 1266 . . 1266 1271 + Cac8I GCNNGC 1268 1268 . . 1269 1272 + CviJI RGCY 1270 1270 . . 1270 1280 + MwoI GCNNNNNNNGC 1276 1273 . . 1277 1280 + AciI CCGC 1277 1279 . . 1277 1282 + BsrBI CCGCTC 1279 1279 . . 1294 1298 + BccI CCATC 1302 1303 . . 1298 1302 + BccI CCATC 1306 1307 . . 1295 1299 - FokI GGATG 1281 1285 . . 1295 1299 - BseGI GGATG 1294 1292 . . 1301 1306 + Hpy178III* TCNNGA 1302 1304 . . 1301 1306 + NruI TCGCGA 1303 1303 . . 1302 1305 + FnuDII* CGCG 1303 1303 . . 1299 1304 - BtgZI GCGATG 1284 1288 . . 1306 1311 + AclI AACGTT 1307 1309 . . 1307 1310 + TaiI ACGT 1310 1306 . . 1307 1310 + SetI ASST 1310 1306 . . 1307 1310 + MaeII ACGT 1307 1309 . . 1318 1322 + BccI CCATC 1326 1327 . . 1318 1328 + BsiYI* CCNNNNNNNGG 1324 1321 . . 1318 1328 + PflMI CCANNNNNTGG 1324 1321 . . 1327 1330 + CviJI RGCY 1328 1328 . . 1327 1330 + HaeIII GGCC 1328 1328 . . 1327 1331 + BmgT120I GGNCC 1328 1329 . . 1327 1331 + AsuI* GGNCC 1327 1330 . . 1329 1339 + BsiYI* CCNNNNNNNGG 1335 1332 . . 1330 1340 + BsiYI* CCNNNNNNNGG 1336 1333 . . 1334 1340 + PasI CCCWGGG 1335 1338 . . 1334 1339 + SecI* CCNNGG 1334 1338 . . 1335 1339 + BssKI CCNGG 1334 1339 . . 1335 1339 + BseBI CCWGG 1336 1337 . . 1335 1340 + SecI* CCNNGG 1335 1339 . . 1335 1339 + ScrFI CCNGG 1336 1337 . . 1335 1339 + EcoRII CCWGG 1334 1339 . . 1337 1342 - TaqII CACCCA 1327 1325 . . 1347 1350 + SetI ASST 1350 1346 . . 1354 1358 + BlsI GCNGC 1356 1355 . . 1354 1358 + BisI GCNGC 1355 1356 . . 1354 1358 + TseI GCWGC 1354 1357 . . 1354 1358 - BbvI GCAGC 1341 1345 . . 1355 1360 - EcoP15I CAGCAG 1327 1329 . . 1365 1370 - TsoI TARCCA 1355 1353 . . 1399 1402 + TspEI AATT 1398 1402 . . 1412 1416 + BssKI CCNGG 1411 1416 . . 1412 1415 + HpaII CCGG 1412 1414 . . 1412 1417 + SecI* CCNNGG 1412 1416 . . 1412 1416 + ScrFI CCNGG 1413 1414 . . 1412 1416 + CauII* CCSGG 1413 1414 . . 1423 1426 + FatI CATG 1422 1426 . . 1423 1426 + NlaIII CATG 1426 1422 . . 1423 1426 + CviAII CATG 1423 1425 . . 1421 1425 - TspDTI ATGAA 1411 1409 . . 1426 1436 + MwoI GCNNNNNNNGC 1432 1429 . . 1432 1437 + HaeII RGCGCY 1436 1432 . . 1432 1437 + Eco47III AGCGCT 1434 1434 . . 1433 1436 + Hin6I GCGC 1433 1435 . . 1433 1436 + GlaI GCGC 1434 1434 . . 1433 1436 + HhaI GCGC 1435 1433 . . 1435 1439 + BlsI GCNGC 1437 1436 . . 1435 1439 + BisI GCNGC 1436 1437 . . 1435 1439 + TseI GCWGC 1435 1438 . . 1435 1439 - BbvI GCAGC 1422 1426 . . 1446 1450 - MboII GAAGA 1438 1437 . . 1452 1455 + CviRI* TGCA 1453 1453 . . 1453 1457 + BlsI GCNGC 1455 1454 . . 1453 1457 + BisI GCNGC 1454 1455 . . 1453 1457 + BbvI GCAGC 1465 1469 . . 1453 1457 + TseI GCWGC 1453 1456 . . 1454 1459 + EcoP15I CAGCAG 1484 1486 . . 1456 1460 + BlsI GCNGC 1458 1457 . . 1456 1460 + BisI GCNGC 1457 1458 . . 1456 1460 + BbvI GCAGC 1468 1472 . . 1456 1460 + TseI GCWGC 1456 1459 . . 1458 1461 + SetI ASST 1461 1457 . . 1458 1461 + CviJI RGCY 1459 1459 . . 1458 1461 + AluI AGCT 1459 1459 . . 1463 1466 + TaqI TCGA 1463 1465 . . 1467 1476 + MslI CAYNNNNRTG 1471 1471 . . 1469 1472 + MaeI CTAG 1469 1471 . . 1480 1483 + FatI CATG 1479 1483 . . 1480 1483 + NlaIII CATG 1483 1479 . . 1480 1483 + CviAII CATG 1480 1482 . . 1481 1485 + TspDTI ATGAA 1496 1494 . . 1482 1493 + BdaI TGANNNNNNTCA 1471 1469 1505 1503 1485 1488 + CviJI RGCY 1486 1486 . . 1490 1494 + DdeI CTNAG 1490 1493 . . 1490 1494 + BspCNI CTCAG 1503 1501 . . 1490 1494 + BseMII CTCAG 1504 1502 . . 1491 1495 + Hpy188I TCNGA 1493 1492 . . 1482 1493 - BdaI TGANNNNNNTCA 1505 1503 1471 1469 1494 1498 + MboII GAAGA 1506 1505 . . 1504 1509 + TatI WGTACW 1504 1508 . . 1505 1508 + Csp6I GTAC 1505 1507 . . 1505 1508 + RsaI GTAC 1506 1506 . . 1521 1526 + SpeI ACTAGT 1521 1525 . . 1522 1525 + MaeI CTAG 1522 1524 . . 1518 1522 - BsmAI GTCTC 1512 1516 . . 1541 1545 + SfaNI GCATC 1550 1554 . . 1544 1548 + Hpy188I TCNGA 1546 1545 . . 1551 1556 + Hpy166II GTNNAC 1553 1553 . . 1558 1562 + BccI CCATC 1566 1567 . . 1559 1563 - FokI GGATG 1545 1549 . . 1563 1566 + FatI CATG 1562 1566 . . 1563 1566 + NlaIII CATG 1566 1562 . . 1563 1566 + CviAII CATG 1563 1565 . . 1559 1563 - BseGI GGATG 1558 1556 . . 1565 1568 + CviRI* TGCA 1566 1566 . . 1566 1576 + MwoI GCNNNNNNNGC 1572 1569 . . 1574 1577 + CviJI RGCY 1575 1575 . . 1571 1575 - Hin4II* CCTTC 1565 1564 . . 1577 1580 + MseI TTAA 1577 1579 . . 1600 1604 + MboII GAAGA 1612 1611 . . 1600 1605 + BbvII* GAAGAC 1607 1611 . . 1609 1613 - SfaNI GCATC 1599 1603 . . 1615 1618 + MseI TTAA 1615 1617 . . 1620 1623 + SetI ASST 1623 1619 . . 1622 1627 + Eco57MI CTGRAG 1643 1641 . . 1622 1627 + Eco57I CTGAAG 1643 1641 . . 1624 1628 + MboII GAAGA 1636 1635 . . 1643 1646 + MaeI CTAG 1643 1645 . . 1659 1664 + Hpy178III* TCNNGA 1660 1662 . . 1676 1680 + Hpy188I TCNGA 1678 1677 . . 1679 1683 + MboII GAAGA 1691 1690 . . 1679 1684 - Ksp632I* CTCTTC 1674 1677 . . 1687 1692 + SalI GTCGAC 1687 1691 . . 1687 1692 + Hpy166II GTNNAC 1689 1689 . . 1687 1692 + HindII GTYRAC 1689 1689 . . 1687 1692 + AccI GTMKAC 1688 1690 . . 1688 1691 + TaqI TCGA 1688 1690 . . 1703 1707 + FokI GGATG 1716 1720 . . 1703 1707 + BseGI GGATG 1709 1707 . . 1708 1711 + MseI TTAA 1708 1710 . . 1716 1719 + TspEI AATT 1715 1719 . . 1731 1734 + CviJI RGCY 1732 1732 . . 1732 1736 + TauI GCSGC 1735 1732 . . 1732 1736 + BlsI GCNGC 1734 1733 . . 1732 1736 + BisI GCNGC 1733 1734 . . 1733 1736 + AciI CCGC 1733 1735 . . 1738 1742 + TspGWI ACGGA 1753 1751 . . 1743 1747 - Hin4II* CCTTC 1737 1736 . . 1757 1761 + BmgT120I GGNCC 1758 1759 . . 1757 1761 + AvaII GGWCC 1757 1760 . . 1757 1761 + AsuI* GGNCC 1757 1760 . . 1761 1767 + TspRI CASTGNN 1767 1758 . . 1762 1766 + BsrI ACTGG 1767 1765 . . 1766 1770 + TfiI GAWTC 1766 1769 . . 1766 1770 + HinfI GANTC 1766 1769 . . 1766 1776 + Hin4I GAYNNNNNVTC 1757 1752 1789 1784 1769 1772 + TaqI TCGA 1769 1771 . . 1771 1776 + EcoRV GATATC 1773 1773 . . 1776 1782 + TspRI CASTGNN 1782 1773 . . 1766 1776 - Hin4I GAYNNNNNVTC 1789 1784 1757 1752 1777 1781 + Tsp4CI* ACNGT 1779 1778 . . 1784 1787 + MnlI CCTC 1794 1793 . . 1786 1790 + Hpy188I TCNGA 1788 1787 . . 1790 1793 + TspEI AATT 1789 1793 . . 1791 1796 + VspI ATTAAT 1792 1794 . . 1792 1795 + MseI TTAA 1792 1794 . . 1796 1801 + BalI TGGCCA 1798 1798 . . 1796 1801 + CfrI YGGCCR 1796 1800 . . 1797 1800 + CviJI RGCY 1798 1798 . . 1797 1800 + HaeIII GGCC 1798 1798 . . 1798 1808 + MwoI GCNNNNNNNGC 1804 1801 . . 1806 1809 + SetI ASST 1809 1805 . . 1806 1809 + CviJI RGCY 1807 1807 . . 1806 1809 + AluI AGCT 1807 1807 . . 1818 1823 + HindIII AAGCTT 1818 1822 . . 1819 1822 + SetI ASST 1822 1818 . . 1819 1822 + CviJI RGCY 1820 1820 . . 1819 1822 + AluI AGCT 1820 1820 . . 1825 1830 + BsiI* CACGAG 1825 1829 . . 1831 1835 + TspDTI ATGAA 1846 1844 . . 1833 1837 - Hin4II* CCTTC 1827 1826 . . 1839 1842 + TaiI ACGT 1842 1838 . . 1839 1842 + SetI ASST 1842 1838 . . 1839 1842 + MaeII ACGT 1839 1841 . . 1847 1851 + HgaI GACGC 1856 1861 . . 1851 1854 + FatI CATG 1850 1854 . . 1851 1854 + NlaIII CATG 1854 1850 . . 1851 1854 + CviAII CATG 1851 1853 . . 1853 1858 - TsoI TARCCA 1843 1841 . . 1885 1889 + MaeIII GTNAC 1884 1889 . . 1882 1886 - BsrI ACTGG 1882 1880 . . 1888 1892 + Tsp4CI* ACNGT 1890 1889 . . 1894 1898 + MboII GAAGA 1906 1905 . . 1903 1906 + CviJI RGCY 1904 1904 . . 1907 1910 + Csp6I GTAC 1907 1909 . . 1907 1910 + RsaI GTAC 1908 1908 . . 1909 1914 + Cfr10I RCCGGY 1909 1913 . . 1909 1914 + BetI* WCCGGW 1909 1913 . . 1909 1914 + AgeI ACCGGT 1909 1913 . . 1910 1913 + HpaII CCGG 1910 1912 . . 1913 1918 + SduI GDGCHC 1917 1913 . . 1913 1918 + HgiAI* GWGCWC 1917 1913 . . 1915 1925 + MwoI GCNNNNNNNGC 1921 1918 . . 1917 1921 + Hpy188I TCNGA 1919 1918 . . 1923 1926 + CviRI* TGCA 1924 1924 . . 1931 1936 + BsaAI YACGTR 1933 1933 . . 1932 1935 + TaiI ACGT 1935 1931 . . 1932 1935 + SetI ASST 1935 1931 . . 1932 1935 + MaeII ACGT 1932 1934 . . 1934 1938 + Tsp45I GTSAC 1933 1938 . . 1934 1938 + MaeIII GTNAC 1933 1938 . . 1936 1940 + HgaI GACGC 1945 1950 . . 1945 1955 + FalI AAGNNNNNCTT 1936 1931 1968 1963 1946 1949 + CviJI RGCY 1947 1947 . . 1954 1957 + MseI TTAA 1954 1956 . . 1945 1955 - FalI AAGNNNNNCTT 1968 1963 1936 1931 1958 1961 + CviRI* TGCA 1959 1959 . . 1972 1978 + SanDI GGGWCCC 1973 1976 . . 1972 1978 + PpuMI RGGWCCY 1973 1976 . . 1972 1978 + DraII RGGNCCY 1973 1976 . . 1972 1976 + BslFI GGGAC 1986 1990 . . 1972 1977 + NlaIV GGNNCC 1974 1974 . . 1969 1973 - Hin4II* CCTTC 1963 1962 . . 1973 1978 + NlaIV GGNNCC 1975 1975 . . 1973 1977 + BmgT120I GGNCC 1974 1975 . . 1973 1977 + AvaII GGWCC 1973 1976 . . 1973 1977 + AsuI* GGNCC 1973 1976 . . 1977 1987 + BsiYI* CCNNNNNNNGG 1983 1980 . . 1987 1992 + Hpy166II GTNNAC 1989 1989 . . 1996 2000 + BmgT120I GGNCC 1997 1998 . . 1996 2000 + AvaII GGWCC 1996 1999 . . 1996 2000 + AsuI* GGNCC 1996 1999 . . 2015 2019 + BccI CCATC 2023 2024 . . 2021 2024 + Hin6I GCGC 2021 2023 . . 2021 2024 + GlaI GCGC 2022 2022 . . 2021 2024 + HhaI GCGC 2023 2021 . . 2023 2027 + SfaNI GCATC 2032 2036 . . 2034 2037 + TspEI AATT 2033 2037 . . 2037 2040 + CviRI* TGCA 2038 2038 . . 2044 2047 + MseI TTAA 2044 2046 . . 2056 2061 + HindIII AAGCTT 2056 2060 . . 2057 2060 + SetI ASST 2060 2056 . . 2057 2060 + CviJI RGCY 2058 2058 . . 2057 2060 + AluI AGCT 2058 2058 . . 2068 2072 + Hpy188I TCNGA 2070 2069 . . 2083 2086 + MseI TTAA 2083 2085 . . 2100 2111 + BstXI CCANNNNNNTGG 2107 2103 . . 2097 2101 - FokI GGATG 2083 2087 . . 2101 2104 + FatI CATG 2100 2104 . . 2101 2104 + NlaIII CATG 2104 2100 . . 2101 2104 + CviAII CATG 2101 2103 . . 2097 2101 - BseGI GGATG 2096 2094 . . 2102 2106 + TspDTI ATGAA 2117 2115 . . 2106 2109 + SetI ASST 2109 2105 . . 2106 2112 + SexAI ACCWGGT 2106 2111 . . 2107 2111 + BssKI CCNGG 2106 2111 . . 2107 2111 + BseBI CCWGG 2108 2109 . . 2107 2111 + ScrFI CCNGG 2108 2109 . . 2107 2111 + EcoRII CCWGG 2106 2111 . . 2109 2114 - TsoI TARCCA 2099 2097 . . 2119 2123 + MaeIII GTNAC 2118 2123 . . 2123 2128 + Eco57MI CTGRAG 2144 2142 . . 2123 2128 + Eco57I CTGAAG 2144 2142 . . 2132 2136 + TfiI GAWTC 2132 2135 . . 2132 2136 + HinfI GANTC 2132 2135 . . 2135 2138 + TaqI TCGA 2135 2137 . . 2144 2149 + DsaI* CCRYGG 2144 2148 . . 2144 2149 + StyI CCWWGG 2144 2148 . . 2144 2149 + NcoI CCATGG 2144 2148 . . 2144 2149 + SecI* CCNNGG 2144 2148 . . 2145 2148 + FatI CATG 2144 2148 . . 2145 2148 + NlaIII CATG 2148 2144 . . 2145 2148 + CviAII CATG 2145 2147 . . 2147 2152 - TaqII CACCCA 2137 2135 . . 2152 2156 + HphI GGTGA 2164 2163 . . 2159 2163 + TfiI GAWTC 2159 2162 . . 2159 2163 + HinfI GANTC 2159 2162 . . 2167 2172 + EcoRV GATATC 2169 2169 . . 2183 2186 + MnlI CCTC 2193 2192 . . 2185 2190 + Hpy178III* TCNNGA 2186 2188 . . 2193 2198 + NlaIV GGNNCC 2195 2195 . . 2193 2198 + HgiCI* GGYRCC 2193 2197 . . 2202 2205 + CviRI* TGCA 2203 2203 . . 2200 2204 - SfaNI GCATC 2190 2194 . . 2211 2215 + DdeI CTNAG 2211 2214 . . 2221 2225 + Tsp4CI* ACNGT 2223 2222 . . 2229 2232 + SetI ASST 2232 2228 . . 2229 2232 + CviJI RGCY 2230 2230 . . 2229 2232 + AluI AGCT 2230 2230 . . 2242 2251 + MslI CAYNNNNRTG 2246 2246 . . 2242 2245 + FatI CATG 2241 2245 . . 2242 2245 + NlaIII CATG 2245 2241 . . 2242 2245 + CviAII CATG 2242 2244 . . 2247 2250 + SetI ASST 2250 2246 . . 2246 2249 - MnlI CCTC 2239 2238 . . 2249 2254 + SduI GDGCHC 2253 2249 . . 2249 2254 + HgiAI* GWGCWC 2253 2249 . . 2261 2264 + MseI TTAA 2261 2263 . . 2264 2267 + CviJI RGCY 2265 2265 . . 2266 2270 + BssKI CCNGG 2265 2270 . . 2266 2270 + ScrFI CCNGG 2267 2268 . . 2266 2270 + CauII* CCSGG 2267 2268 . . 2267 2270 + HpaII CCGG 2267 2269 . . 2296 2299 - MnlI CCTC 2289 2288 . . 2305 2310 + ClaI ATCGAT 2306 2308 . . 2306 2309 + TaqI TCGA 2306 2308 . . 2307 2318 + BcgI CGANNNNNNTGC 2296 2294 2330 2328 2318 2323 + StyI CCWWGG 2318 2322 . . 2318 2323 + SecI* CCNNGG 2318 2322 . . 2332 2335 + TspEI AATT 2331 2335 . . 2339 2342 + MseI TTAA 2339 2341 . . 2336 2341 - TsoI TARCCA 2326 2324 . . 2361 2366 + AclI AACGTT 2362 2364 . . 2362 2365 + TaiI ACGT 2365 2361 . . 2362 2365 + SetI ASST 2365 2361 . . 2362 2365 + MaeII ACGT 2362 2364 . . 2374 2378 - BsrI ACTGG 2374 2372 . . 2407 2411 + HinfI GANTC 2407 2410 . . 2407 2411 - PleI GAGTC 2401 2402 . . 2407 2411 - MlyI GAGTC 2401 2401 . . 2426 2429 + SetI ASST 2429 2425 . . 2425 2428 - MnlI CCTC 2418 2417 . . 2432 2437 + Hpy178III* TCNNGA 2433 2435 . . 2440 2444 + BlsI GCNGC 2442 2441 . . 2440 2444 + BisI GCNGC 2441 2442 . . 2440 2444 + BbvI GCAGC 2452 2456 . . 2440 2444 + TseI GCWGC 2440 2443 . . 2442 2445 + CviJI RGCY 2443 2443 . . 2443 2453 + MwoI GCNNNNNNNGC 2449 2446 . . 2448 2453 + BsmI GAATGC 2454 2452 . . 2452 2457 + Cac8I GCNNGC 2454 2454 . . 2456 2459 + Hin6I GCGC 2456 2458 . . 2456 2459 + GlaI GCGC 2457 2457 . . 2456 2459 + HhaI GCGC 2458 2456 . . 2458 2462 + SfaNI GCATC 2467 2471 . . 2458 2468 + MwoI GCNNNNNNNGC 2464 2461 . . 2458 2468 + BstAPI GCANNNNNTGC 2464 2461 . . 2464 2467 + FatI CATG 2463 2467 . . 2464 2467 + NlaIII CATG 2467 2463 . . 2464 2467 + CviAII CATG 2464 2466 . . 2470 2474 + MaeIII GTNAC 2469 2474 . . 2467 2471 - BceAI ACGGC 2452 2454 . . 2482 2488 + TspRI CASTGNN 2488 2479 . . 2483 2487 + BsrI ACTGG 2488 2486 . . 2486 2490 + FokI GGATG 2499 2503 . . 2486 2490 + BseGI GGATG 2492 2490 . . 2487 2491 - BccI CCATC 2481 2482 . . 2493 2496 + SetI ASST 2496 2492 . . 2491 2495 - Hin4II* CCTTC 2485 2484 . . 2486 2497 - TstI CACNNNNNNTCC 2510 2505 2478 2473 2502 2505 + SetI ASST 2505 2501 . . 2504 2509 + BsgI GTGCAG 2525 2523 . . 2505 2508 + CviRI* TGCA 2506 2506 . . 2513 2516 + TspEI AATT 2512 2516 . . 2520 2525 + NspI RCATGY 2524 2520 . . 2521 2524 + FatI CATG 2520 2524 . . 2521 2524 + NlaIII CATG 2524 2520 . . 2521 2524 + CviAII CATG 2521 2523 . . 2535 2538 + CviRI* TGCA 2536 2536 . . 2533 2537 - SfaNI GCATC 2523 2527 . . 2558 2561 + MseI TTAA 2558 2560 . . 2568 2571 + Csp6I GTAC 2568 2570 . . 2568 2571 + RsaI GTAC 2569 2569 . . 2571 2575 + Hpy99I CGWCG 2575 2570 . . 2572 2577 + ZraI GACGTC 2574 2574 . . 2572 2577 + AcyI GRCGYC 2573 2575 . . 2572 2577 + AatII GACGTC 2576 2572 . . 2573 2576 + TaiI ACGT 2576 2572 . . 2573 2576 + SetI ASST 2576 2572 . . 2573 2576 + MaeII ACGT 2573 2575 . . 2581 2584 + SetI ASST 2584 2580 . . 2581 2584 + CviJI RGCY 2582 2582 . . 2581 2584 + AluI AGCT 2582 2582 . . 2585 2589 + BccI CCATC 2593 2594 . . 2588 2591 + TaqI TCGA 2588 2590 . . 2590 2593 - MnlI CCTC 2583 2582 . . 2596 2601 + HindIII AAGCTT 2596 2600 . . 2597 2600 + SetI ASST 2600 2596 . . 2597 2600 + CviJI RGCY 2598 2598 . . 2597 2600 + AluI AGCT 2598 2598 . . 2606 2610 + SfaNI GCATC 2615 2619 . . 2613 2627 + XcmI CCANNNNNNNNNTGG 2620 2619 . . 2639 2642 + TaqI TCGA 2639 2641 . . 2647 2651 - HphI GGTGA 2639 2638 . . 2655 2658 + CviJI RGCY 2656 2656 . . 2674 2677 + SetI ASST 2677 2673 . . 2689 2692 + CviJI RGCY 2690 2690 . . 2700 2704 - MboII GAAGA 2692 2691 . . 2705 2709 + BccI CCATC 2713 2714 . . 2717 2721 + DdeI CTNAG 2717 2720 . . 2726 2731 + SspI AATATT 2728 2728 . . 2730 2734 - TspDTI ATGAA 2720 2718 . . 2740 2743 + FatI CATG 2739 2743 . . 2740 2743 + NlaIII CATG 2743 2739 . . 2740 2743 + CviAII CATG 2740 2742 . . 2743 2746 + DpnI GATC 2744 2744 . . 2743 2746 + BstKTI GATC 2745 2743 . . 2743 2746 + MboI GATC 2742 2746 . . 2746 2750 + BccI CCATC 2754 2755 . . 2743 2747 - BinI* GGATC 2737 2738 . . 2752 2755 + MseI TTAA 2752 2754 . . 2765 2768 + MaeI CTAG 2765 2767 . . 2769 2772 + SetI ASST 2772 2768 . . 2768 2771 - MnlI CCTC 2761 2760 . . 2776 2779 + TaiI ACGT 2779 2775 . . 2776 2779 + SetI ASST 2779 2775 . . 2776 2779 + MaeII ACGT 2776 2778 . . 2781 2791 + BsiYI* CCNNNNNNNGG 2787 2784 . . 2783 2787 + BccI CCATC 2791 2792 . . 2790 2794 + FokI GGATG 2803 2807 . . 2790 2794 + BseGI GGATG 2796 2794 . . 2799 2802 + SetI ASST 2802 2798 . . 2799 2802 + CviJI RGCY 2800 2800 . . 2799 2802 + AluI AGCT 2800 2800 . . 2808 2811 + SetI ASST 2811 2807 . . 2810 2815 + BsgI GTGCAG 2831 2829 . . 2811 2814 + CviRI* TGCA 2812 2812 . . 2814 2817 + SetI ASST 2817 2813 . . 2812 2817 - BspMI ACCTGC 2803 2807 . . 2827 2837 + MwoI GCNNNNNNNGC 2833 2830 . . 2832 2835 + CviJI RGCY 2833 2833 . . 2833 2837 + BlsI GCNGC 2835 2834 . . 2833 2837 + BisI GCNGC 2834 2835 . . 2833 2837 + TseI GCWGC 2833 2836 . . 2834 2839 + PstI CTGCAG 2838 2834 . . 2834 2839 + SfeI* CTRYAG 2834 2838 . . 2835 2838 + CviRI* TGCA 2836 2836 . . 2833 2837 - BbvI GCAGC 2820 2824 . . 2840 2845 + ApoI RAATTY 2840 2844 . . 2841 2844 + TspEI AATT 2840 2844 . . 2854 2858 + BssKI CCNGG 2853 2858 . . 2854 2857 + HpaII CCGG 2854 2856 . . 2854 2858 + ScrFI CCNGG 2855 2856 . . 2854 2858 + CauII* CCSGG 2855 2856 . . 2863 2866 + TaqI TCGA 2863 2865 . . 2867 2872 + NdeI CATATG 2868 2870 . . 2876 2880 + MaeIII GTNAC 2875 2880 . . 2874 2879 - TsoI TARCCA 2864 2862 . . 2880 2883 + FatI CATG 2879 2883 . . 2880 2883 + NlaIII CATG 2883 2879 . . 2880 2883 + CviAII CATG 2880 2882 . . 2893 2898 + SalI GTCGAC 2893 2897 . . 2893 2898 + Hpy166II GTNNAC 2895 2895 . . 2893 2898 + HindII GTYRAC 2895 2895 . . 2893 2898 + AccI GTMKAC 2894 2896 . . 2894 2897 + TaqI TCGA 2894 2896 . . 2895 2899 + Hpy99I CGWCG 2899 2894 . . 2898 2902 + Hpy99I CGWCG 2902 2897 . . 2901 2905 + Hpy99I CGWCG 2905 2900 . . 2903 2907 + Tsp4CI* ACNGT 2905 2904 . . 2905 2909 + HphI GGTGA 2917 2916 . . 2910 2913 + TspEI AATT 2909 2913 . . 2916 2919 + SetI ASST 2919 2915 . . 2914 2918 - Hin4II* CCTTC 2908 2907 . . 2925 2928 + MseI TTAA 2925 2927 . . #--------------------------------------- # # # AatII: # [GACGTC] # 352 2576 # # AccI: # [GTMKAC] # 34 287 1206 1401 # # AciI: # [CCGC] # 66 331 337 456 543 1195 # # AclI: # [AACGTT] # 566 1055 1307 # # AcyI: # [GRCGYC] # 355 776 1797 # # AflII: # [CTTAAG] # 178 2750 # # AgeI: # [ACCGGT] # 1019 1909 # # AhaIII*: # [TTTAAA] # 447 717 1764 # # AluI: # [AGCT] # 13 16 18 32 65 70 # 91 128 150 172 177 202 # 225 229 238 348 352 402 # # ApoI: # [RAATTY] # 88 1145 1695 # # AsuI*: # [GGNCC] # 23 216 224 414 430 689 # 932 # # AvaI: # [CYCGRG] # 30 2898 # # AvaII: # [GGWCC] # 23 216 844 913 932 # # BalI: # [TGGCCA] # 1130 1798 # # BbvI: # [GCAGC] # 3 43 81 108 203 368 # 399 739 984 # # BbvII*: # [GAAGAC] # 1321 1607 # # BccI: # [CCATC] # 4 20 37 41 47 112 # 117 120 137 240 407 457 # 458 731 # # BceAI: # [ACGGC] # 189 380 476 574 1309 # # BcgI: # [CGANNNNNNTGC] # 34 598 2296 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 544 893 1423 # # BetI*: # [WCCGGW] # 1019 1909 # # BinI*: # [GGATC] # 191 2737 # # BisI: # [GCNGC] # 3 18 81 94 228 276 # 393 399 708 728 # # BlsI: # [GCNGC] # 3 18 81 93 228 276 # 393 399 708 729 # # BmeT110I: # [CYCGRG] # 31 2897 # # BmgT120I: # [GGNCC] # 23 216 225 414 430 689 # 931 # # Bpu10I: # [CCTNAGC] # 1067 1861 # # BsaAI: # [YACGTR] # 4 245 558 995 1126 # # BsaBI: # [GATNNNNATC] # 1173 1755 # # BsaXI: # [ACNNNNNCTCC] # 30 1206 1692 # # BseBI: # [CCWGG] # 434 772 820 902 # # BseGI: # [GGATG] # 77 132 151 160 183 264 # 304 387 396 874 # # BseMII: # [CTCAG] # 432 1072 1424 # # BseRI: # [GAGGAG] # 1105 1823 # # BsgI: # [GTGCAG] # 97 306 1240 1285 # # BsiI*: # [CACGAG] # 1103 1825 # # BsiYI*: # [CCNNNNNNNGG] # 1 11 141 187 236 647 # 804 901 # # BslFI: # [GGGAC] # 942 1986 # # BsmAI: # [GTCTC] # 3 705 804 1416 # # BsmI: # [GAATGC] # 474 657 1797 # # BspCNI: # [CTCAG] # 432 1071 1425 # # BspMI: # [ACCTGC] # 125 1198 1605 # # BsrBI: # [CCGCTC] # 1279 1649 # # BsrDI: # [GCAATG] # 288 401 2239 # # BsrI: # [ACTGG] # 36 114 115 225 440 492 # 740 766 # # BssKI: # [CCNGG] # 75 77 159 434 588 695 # 900 # # BstAPI: # [GCANNNNNTGC] # 464 2464 # # BstKTI: # [GATC] # 183 607 2138 # # BstXI: # [CCANNNNNNTGG] # 821 2107 # # BtgZI: # [GCGATG] # 1284 1644 # # Cac8I: # [GCNNGC] # 92 474 536 640 1186 # # CauII*: # [CCSGG] # 73 588 854 1413 # # Cfr10I: # [RCCGGY] # 1019 1909 # # CfrI: # [YGGCCR] # 668 1128 1132 # # ClaI: # [ATCGAT] # 622 2306 # # Csp6I: # [GTAC] # 138 246 248 281 281 311 # 360 402 661 # # CspCI: # [CAANNNNNGTGG] # 35 419 2474 # # CviAII: # [CATG] # 19 22 44 48 57 57 # 83 91 97 140 148 219 # 222 250 285 288 858 # # CviJI: # [RGCY] # 8 9 13 16 18 18 # 21 27 28 32 33 34 # 35 42 43 49 58 58 # 65 66 69 70 73 84 # 89 95 102 110 111 131 # 139 140 150 157 160 172 # 178 225 # # CviRI*: # [TGCA] # 24 30 35 59 79 90 # 92 113 152 165 187 276 # 303 358 452 513 # # DdeI: # [CTNAG] # 9 41 211 423 506 721 # 1017 # # DinI: # [GGCGCC] # 777 2151 # # DpnI: # [GATC] # 184 606 2138 # # DraII: # [RGGNCCY] # 955 1973 # # DsaI*: # [CCRYGG] # 375 404 784 1365 # # Ecl136II: # [GAGCTC] # 1057 1871 # # Eco31I: # [GGTCTC] # 708 2220 # # Eco47III: # [AGCGCT] # 1434 1494 # # Eco57I: # [CTGAAG] # 344 501 624 675 784 # # Eco57MI: # [CTGRAG] # 344 501 624 675 784 # # EcoP15I: # [CAGCAG] # 157 341 986 1444 # # EcoRII: # [CCWGG] # 434 772 822 900 # # EcoRV: # [GATATC] # 396 759 816 957 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 98 187 960 # 1587 # # FatI: # [CATG] # 19 22 44 49 57 57 # 83 90 97 140 148 219 # 222 250 285 288 858 # # FnuDII*: # [CGCG] # 405 898 1625 # # FokI: # [GGATG] # 97 125 160 170 171 264 # 304 367 416 854 # # GlaI: # [GCGC] # 58 435 471 588 657 719 # # HaeII: # [RGCGCY] # 657 779 1492 # # HaeIII: # [GGCC] # 49 177 198 470 904 1130 # # HgaI: # [GACGC] # 89 394 983 1462 # # HgiAI*: # [GWGCWC] # 336 675 858 1059 # # HgiCI*: # [GGYRCC] # 735 775 1418 # # HgiJII*: # [GRGCYC] # 1059 1869 # # HhaI: # [GCGC] # 59 435 470 588 657 719 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 212 352 1129 1139 # # Hin4II*: # [CCTTC] # 20 90 136 172 185 277 # 337 423 522 766 # # Hin6I: # [GCGC] # 57 435 472 588 657 719 # # HindII: # [GTYRAC] # 33 1206 1689 # # HindIII: # [AAGCTT] # 91 175 238 332 540 719 # 833 # # HinfI: # [GANTC] # 27 38 47 74 86 114 # 132 235 248 366 495 521 # 545 # # HpaII: # [CCGG] # 74 357 498 587 1412 # # HphI: # [GGTGA] # 11 278 363 384 475 496 # 921 # # Hpy166II: # [GTNNAC] # 33 136 153 288 300 906 # 1112 # # Hpy178III*: # [TCNNGA] # 8 59 67 124 134 247 # 358 495 526 910 # # Hpy188I: # [TCNGA] # 23 53 57 110 131 132 # 140 151 159 222 273 619 # 858 # # Hpy99I: # [CGWCG] # 3 3 23 24 324 595 # 1956 # # KasI: # [GGCGCC] # 775 2153 # # Ksp632I*: # [CTCTTC] # 664 1010 1254 # # MaeI: # [CTAG] # 53 121 163 291 1122 1178 # # MaeII: # [ACGT] # 4 57 93 111 152 203 # 207 211 244 294 390 430 # 532 # # MaeIII: # [GTNAC] # 25 44 49 53 57 185 # 254 280 351 406 1224 # # MboI: # [GATC] # 186 604 2138 # # MboII: # [GAAGA] # 24 55 68 106 215 217 # 236 351 429 441 786 # # MfeI: # [CAATTG] # 626 2302 # # MlyI: # [GAGTC] # 132 144 229 527 710 1186 # # MmeI: # [TCCRAC] # 66 531 646 1685 # # MnlI: # [CCTC] # 26 46 50 70 89 129 # 162 165 167 178 219 256 # 261 399 711 # # MseI: # [TTAA] # 3 6 15 38 39 70 # 78 84 90 93 103 123 # 162 173 178 183 194 219 # 267 396 414 # # MslI: # [CAYNNNNRTG] # 413 682 775 1058 # # MwoI: # [GCNNNNNNNGC] # 15 22 65 95 117 140 # 156 199 232 369 528 990 # # NarI: # [GGCGCC] # 776 2152 # # NcoI: # [CCATGG] # 375 784 1769 # # NdeI: # [CATATG] # 60 2868 # # NlaIII: # [CATG] # 19 22 44 45 57 57 # 83 94 97 140 148 219 # 222 250 285 288 858 # # NlaIV: # [GGNNCC] # 1 137 220 225 241 311 # 733 1060 # # NmeAIII: # [GCCGAG] # 1113 1815 # # NruI: # [TCGCGA] # 1303 1625 # # NspI: # [RCATGY] # 94 404 2430 # # PasI: # [CCCWGGG] # 1335 1593 # # PflMI: # [CCANNNNNTGG] # 1324 1604 # # PleI: # [GAGTC] # 132 145 229 527 709 1186 # # PpuMI: # [RGGWCCY] # 955 1973 # # PspXI: # [VCTCGAGB] # 30 2898 # # PstI: # [CTGCAG] # 90 2838 # # RsaI: # [GTAC] # 138 247 248 281 281 311 # 359 402 661 # # SacI: # [GAGCTC] # 1059 1869 # # SalI: # [GTCGAC] # 35 1206 1687 # # SanDI: # [GGGWCCC] # 955 1973 # # ScrFI: # [CCNGG] # 73 77 159 434 588 695 # 902 # # SduI: # [GDGCHC] # 336 675 858 1059 # # SecI*: # [CCNNGG] # 1 77 174 246 309 375 # 404 610 732 # # SetI: # [ASST] # 4 4 6 7 8 9 # 9 9 11 13 13 16 # 16 18 18 19 20 20 # 23 26 27 32 40 42 # 46 49 54 59 64 64 # 65 67 71 71 77 93 # 95 102 106 111 115 123 # 125 140 144 150 162 179 # 186 # # SexAI: # [ACCWGGT] # 822 2106 # # SfaNI: # [GCATC] # 49 56 92 158 192 277 # 313 433 1358 # # SfeI*: # [CTRYAG] # 94 2834 # # SmlI: # [CTYRAG] # 30 148 2750 # # SnaBI: # [TACGTA] # 245 562 2121 # # SpeI: # [ACTAGT] # 1407 1521 # # SphI: # [GCATGC] # 94 2834 # # SplI*: # [CGTACG] # 245 2683 # # SspI: # [AATATT] # 200 1158 1570 # # StyI: # [CCWWGG] # 174 375 610 1769 # # TaiI: # [ACGT] # 4 57 93 111 149 203 # 207 211 247 294 390 430 # 532 # # TaqI: # [TCGA] # 7 24 31 34 51 81 # 110 171 224 225 282 366 # 1322 # # TaqII: # [CACCCA] # 108 791 810 1219 # # TatI: # [WGTACW] # 419 526 559 1424 # # TauI: # [GCSGC] # 1193 1735 # # TfiI: # [GAWTC] # 27 74 114 124 366 592 # 769 862 # # TseI: # [GCWGC] # 3 18 81 95 228 393 # 399 727 984 # # TsoI: # [TARCCA] # 64 227 256 291 481 488 # 538 583 # # Tsp45I: # [GTSAC] # 57 349 995 1527 # # Tsp4CI*: # [ACNGT] # 6 23 87 111 205 258 # 265 333 368 590 682 # # TspDTI: # [ATGAA] # 52 76 85 160 208 271 # 271 350 603 852 # # TspEI: # [AATT] # 6 19 25 42 60 69 # 70 74 79 85 101 129 # 147 181 189 212 244 253 # 298 317 328 # # TspGWI: # [ACGGA] # 1175 1753 # # TspRI: # [CASTGNN] # 15 327 336 371 440 706 # 733 # # TstI: # [CACNNNNNNTCC] # 32 418 2478 # # VspI: # [ATTAAT] # 1136 1792 # # XbaI: # [TCTAGA] # 1177 1751 # # XcmI: # [CCANNNNNNNNNTGG] # 112 308 1139 1369 # # XhoI: # [CTCGAG] # 30 2898 # # XmnI: # [GAANNNNTTC] # 986 1942 # # ZraI: # [GACGTC] # 354 2574 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2928 # Reported_sequences: 1 # Reported_hitcount: 782 #---------------------------------------