Restriction Fragments for YBR076C-A



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The currently selected gene/sequence is :

YBR076C-A on chromosome II from coordinates 391617 to 391351.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.22666.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 4 9 + BsaAI YACGTR 6 6 . . 5 8 + TaiI ACGT 8 4 . . 5 8 + SetI ASST 8 4 . . 5 8 + MaeII ACGT 5 7 . . 7 10 + Csp6I GTAC 7 9 . . 7 10 + RsaI GTAC 8 8 . . 14 20 + DraII RGGNCCY 15 18 . . 15 19 + BmgT120I GGNCC 16 17 . . 15 19 + AsuI* GGNCC 15 18 . . 16 19 + CviJI RGCY 17 17 . . 16 19 + HaeIII GGCC 17 17 . . 18 21 + MnlI CCTC 28 27 . . 27 30 + MnlI CCTC 37 36 . . 23 27 - MboII GAAGA 15 14 . . 44 48 + BccI CCATC 52 53 . . 50 53 + SetI ASST 53 49 . . 68 72 + TspGWI ACGGA 83 81 . . 74 78 + SfaNI GCATC 83 87 . . 77 81 - MboII GAAGA 69 68 . . 94 104 + MwoI GCNNNNNNNGC 100 97 . . 95 99 + DdeI CTNAG 95 98 . . 95 99 + BspCNI CTCAG 108 106 . . 95 99 + BseMII CTCAG 109 107 . . 96 100 + Hpy188I TCNGA 98 97 . . 100 106 + DraII RGGNCCY 101 104 . . 101 107 + DraII RGGNCCY 102 105 . . 101 106 + SduI GDGCHC 105 101 . . 101 106 + Bsp120I GGGCCC 101 105 . . 101 106 + NlaIV GGNNCC 103 103 . . 101 105 + BmgT120I GGNCC 102 103 . . 101 106 + HgiJII* GRGCYC 105 101 . . 101 106 + BseSI GKGCMC 105 101 . . 101 105 + AsuI* GGNCC 101 104 . . 101 106 + ApaI GGGCCC 105 101 . . 99 102 - MnlI CCTC 92 91 . . 102 105 + CviJI RGCY 103 103 . . 102 105 + HaeIII GGCC 103 103 . . 102 106 + BmgT120I GGNCC 103 104 . . 102 106 + AsuI* GGNCC 102 105 . . 109 112 + FatI CATG 108 112 . . 109 112 + NlaIII CATG 112 108 . . 109 112 + CviAII CATG 109 111 . . 115 118 + SetI ASST 118 114 . . 115 118 + CviJI RGCY 116 116 . . 115 118 + AluI AGCT 116 116 . . 134 137 + SetI ASST 137 133 . . 134 137 + CviJI RGCY 135 135 . . 134 137 + AluI AGCT 135 135 . . 135 139 + BlsI GCNGC 137 136 . . 135 139 + BisI GCNGC 136 137 . . 135 139 + TseI GCWGC 135 138 . . 135 139 - BbvI GCAGC 122 126 . . 141 145 + Tsp4CI* ACNGT 143 142 . . 143 148 + NlaIV GGNNCC 145 145 . . 143 148 + HgiCI* GGYRCC 143 147 . . 146 156 + MwoI GCNNNNNNNGC 152 149 . . 149 154 + XhoII RGATCY 149 153 . . 149 154 + BglII AGATCT 149 153 . . 150 153 + DpnI GATC 151 151 . . 150 153 + BstKTI GATC 152 150 . . 150 153 + MboI GATC 149 153 . . 162 166 - TspDTI ATGAA 152 150 . . 173 177 + FokI GGATG 186 190 . . 173 177 + BseGI GGATG 179 177 . . 177 183 + BstEII GGTNACC 177 182 . . 178 182 + MaeIII GTNAC 177 182 . . 174 178 - BccI CCATC 168 169 . . 181 185 + Tsp4CI* ACNGT 183 182 . . 185 190 + PsiI TTATAA 187 187 . . 189 194 + ApoI RAATTY 189 193 . . 190 193 + TspEI AATT 189 193 . . 192 197 + AhaIII* TTTAAA 194 194 . . 193 196 + MseI TTAA 193 195 . . 196 206 + FalI AAGNNNNNCTT 187 182 219 214 196 206 - FalI AAGNNNNNCTT 219 214 187 182 203 207 - MboII GAAGA 195 194 . . 209 212 + MaeI CTAG 209 211 . . 212 216 + FokI GGATG 225 229 . . 212 216 + BseGI GGATG 218 216 . . 216 220 + HphI GGTGA 228 227 . . 213 217 - BccI CCATC 207 208 . . 220 225 + SspI AATATT 222 222 . . 236 239 + CviJI RGCY 237 237 . . 239 244 + Esp3I CGTCTC 245 249 . . 240 244 + BsmAI GTCTC 245 249 . . 237 241 - BceAI ACGGC 222 224 . . 260 264 + MaeIII GTNAC 259 264 . . 264 267 + MaeI CTAG 264 266 . . #--------------------------------------- # # # AhaIII*: # [TTTAAA] # 73 194 # # AluI: # [AGCT] # 19 116 132 # # ApaI: # [GGGCCC] # 105 162 # # ApoI: # [RAATTY] # 78 189 # # AsuI*: # [GGNCC] # 1 15 86 165 # # BbvI: # [GCAGC] # 122 145 # # BccI: # [CCATC] # 39 52 60 116 # # BceAI: # [ACGGC] # 45 222 # # BglII: # [AGATCT] # 118 149 # # BisI: # [GCNGC] # 131 136 # # BlsI: # [GCNGC] # 130 137 # # BmgT120I: # [GGNCC] # 1 16 86 164 # # BsaAI: # [YACGTR] # 6 261 # # BseGI: # [GGATG] # 39 49 179 # # BseMII: # [CTCAG] # 109 158 # # BseSI: # [GKGCMC] # 105 162 # # BsmAI: # [GTCTC] # 22 245 # # Bsp120I: # [GGGCCC] # 101 166 # # BspCNI: # [CTCAG] # 108 159 # # BstEII: # [GGTNACC] # 90 177 # # BstKTI: # [GATC] # 115 152 # # Csp6I: # [GTAC] # 7 260 # # CviAII: # [CATG] # 109 158 # # CviJI: # [RGCY] # 13 17 19 30 86 102 # # CviRI*: # [TGCA] # 3 264 # # DdeI: # [CTNAG] # 95 172 # # DpnI: # [GATC] # 116 151 # # DraII: # [RGGNCCY] # 1 15 86 165 # # Esp3I: # [CGTCTC] # 22 245 # # FalI: # [AAGNNNNNCTT] # 0 0 32 48 187 # # FatI: # [CATG] # 108 159 # # FokI: # [GGATG] # 39 42 186 # # HaeIII: # [GGCC] # 17 86 164 # # HgiCI*: # [GGYRCC] # 124 143 # # HgiJII*: # [GRGCYC] # 105 162 # # HphI: # [GGTGA] # 39 228 # # Hpy188I: # [TCNGA] # 98 169 # # MaeI: # [CTAG] # 3 55 209 # # MaeII: # [ACGT] # 5 262 # # MaeIII: # [GTNAC] # 8 82 177 # # MboI: # [GATC] # 118 149 # # MboII: # [GAAGA] # 15 54 72 126 # # MnlI: # [CCTC] # 9 28 55 175 # # MseI: # [TTAA] # 74 193 # # MwoI: # [GCNNNNNNNGC] # 52 100 115 # # NlaIII: # [CATG] # 112 155 # # NlaIV: # [GGNNCC] # 42 103 122 # # PsiI: # [TTATAA] # 80 187 # # RsaI: # [GTAC] # 8 259 # # SduI: # [GDGCHC] # 105 162 # # SetI: # [ASST] # 8 19 45 65 130 # # SfaNI: # [GCATC] # 83 184 # # SspI: # [AATATT] # 45 222 # # TaiI: # [ACGT] # 8 259 # # TseI: # [GCWGC] # 132 135 # # Tsp4CI*: # [ACNGT] # 40 84 143 # # TspDTI: # [ATGAA] # 115 152 # # TspEI: # [AATT] # 78 189 # # TspGWI: # [ACGGA] # 83 184 # # XhoII: # [RGATCY] # 118 149 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 267 # Reported_sequences: 1 # Reported_hitcount: 89 #---------------------------------------