Restriction Fragments for OLA1/YBR025C



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The currently selected gene/sequence is :

OLA1/YBR025C on chromosome II from coordinates 291865 to 290681.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.21680.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + MnlI CCTC 14 13 . . 20 23 + TaqI TCGA 20 22 . . 22 26 + MboII GAAGA 34 33 . . 32 35 + SetI ASST 35 31 . . 49 53 + BssKI CCNGG 48 53 . . 49 53 + BseBI CCWGG 50 51 . . 49 53 + ScrFI CCNGG 50 51 . . 49 53 + EcoRII CCWGG 48 53 . . 51 54 + SetI ASST 54 50 . . 66 69 + CviJI RGCY 67 67 . . 67 72 + Cfr10I RCCGGY 67 71 . . 68 71 + HpaII CCGG 68 70 . . 83 88 + BalI TGGCCA 85 85 . . 83 88 + CfrI YGGCCR 83 87 . . 84 87 + CviJI RGCY 85 85 . . 84 87 + HaeIII GGCC 85 85 . . 97 107 + FalI AAGNNNNNCTT 88 83 120 115 99 104 + Hpy166II GTNNAC 101 101 . . 99 104 + AccI GTMKAC 100 102 . . 103 106 + SetI ASST 106 102 . . 97 107 - FalI AAGNNNNNCTT 120 115 88 83 114 117 + CviJI RGCY 115 115 . . 116 120 + BccI CCATC 124 125 . . 120 131 + TstI CACNNNNNNTCC 111 106 143 138 122 125 + MaeI CTAG 122 124 . . 136 142 + BstEII GGTNACC 136 141 . . 137 141 + MaeIII GTNAC 136 141 . . 141 146 + GsaI CCCAGC 145 141 . . 141 146 + BseYI CCCAGC 141 145 . . 144 147 + SetI ASST 147 143 . . 144 147 + CviJI RGCY 145 145 . . 144 147 + AluI AGCT 145 145 . . 169 172 + DpnI GATC 170 170 . . 169 172 + BstKTI GATC 171 169 . . 169 172 + MboI GATC 168 172 . . 171 176 + Hpy178III* TCNNGA 172 174 . . 169 173 - BinI* GGATC 163 164 . . 175 179 + MboII GAAGA 187 186 . . 180 183 + CviJI RGCY 181 181 . . 196 200 + BccI CCATC 204 205 . . 193 197 - BslFI GGGAC 178 182 . . 234 238 + MboII GAAGA 246 245 . . 234 239 + BbvII* GAAGAC 241 245 . . 240 243 + SetI ASST 243 239 . . 240 243 + CviJI RGCY 241 241 . . 240 243 + AluI AGCT 241 241 . . 244 248 + Hpy188I TCNGA 246 245 . . 255 258 + SetI ASST 258 254 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + AluI AGCT 256 256 . . 265 269 + Tsp4CI* ACNGT 267 266 . . 268 273 + Hpy166II GTNNAC 270 270 . . 290 294 + DdeI CTNAG 290 293 . . 295 300 + NlaIV GGNNCC 297 297 . . 295 300 + HgiCI* GGYRCC 295 299 . . 299 302 + MnlI CCTC 309 308 . . 302 307 - EcoP15I CAGCAG 274 276 . . 307 311 + HphI GGTGA 319 318 . . 312 315 + SetI ASST 315 311 . . 310 314 - Hin4II* CCTTC 304 303 . . 333 337 + BsmAI GTCTC 338 342 . . 341 345 + Hpy188I TCNGA 343 342 . . 344 347 + DpnI GATC 345 345 . . 344 347 + BstKTI GATC 346 344 . . 344 347 + MboI GATC 343 347 . . 350 353 + TaqI TCGA 350 352 . . 352 356 + TfiI GAWTC 352 355 . . 352 356 + HinfI GANTC 352 355 . . 380 383 + TaqI TCGA 380 382 . . 385 389 - SfaNI GCATC 375 379 . . 393 396 + TspEI AATT 392 396 . . 399 409 + BsiYI* CCNNNNNNNGG 405 402 . . 401 404 + TaiI ACGT 404 400 . . 401 404 + SetI ASST 404 400 . . 401 404 + MaeII ACGT 401 403 . . 406 409 - MnlI CCTC 399 398 . . 409 413 + HphI GGTGA 421 420 . . 410 414 + Tsp45I GTSAC 409 414 . . 410 414 + MaeIII GTNAC 409 414 . . 413 416 + TaiI ACGT 416 412 . . 413 416 + SetI ASST 416 412 . . 413 416 + MaeII ACGT 413 415 . . 418 421 + DpnI GATC 419 419 . . 418 421 + BstKTI GATC 420 418 . . 418 421 + MboI GATC 417 421 . . 418 422 - BinI* GGATC 412 413 . . 421 425 - BsrI ACTGG 421 419 . . 426 431 + Hpy178III* TCNNGA 427 429 . . 438 441 + TspEI AATT 437 441 . . 443 446 + MseI TTAA 443 445 . . 444 449 + TsoI TARCCA 460 458 . . 454 458 + DdeI CTNAG 454 457 . . 472 477 + EcoRI GAATTC 472 476 . . 472 477 + ApoI RAATTY 472 476 . . 473 476 + TspEI AATT 472 476 . . 478 488 + MwoI GCNNNNNNNGC 484 481 . . 486 489 + CviJI RGCY 487 487 . . 495 498 + SetI ASST 498 494 . . 493 497 - Hin4II* CCTTC 487 486 . . 513 524 + CspCI CAANNNNNGTGG 501 499 536 534 519 522 + SetI ASST 522 518 . . 518 521 - MnlI CCTC 511 510 . . 553 557 + MboII GAAGA 565 564 . . 549 553 - Hin4II* CCTTC 543 542 . . 581 584 + MseI TTAA 581 583 . . 584 587 + TspEI AATT 583 587 . . 589 592 + MaeI CTAG 589 591 . . 619 625 + PfoI TCCNGGA 619 624 . . 620 624 + BssKI CCNGG 619 624 . . 620 624 + BseBI CCWGG 621 622 . . 620 624 + ScrFI CCNGG 621 622 . . 620 624 + EcoRII CCWGG 619 624 . . 642 645 + TspEI AATT 641 645 . . 654 657 + FatI CATG 653 657 . . 654 657 + NlaIII CATG 657 653 . . 654 657 + CviAII CATG 654 656 . . 663 668 + Hpy166II GTNNAC 665 665 . . 663 668 + HindII GTYRAC 665 665 . . 670 676 + EspI* GCTNAGC 671 674 . . 671 675 + DdeI CTNAG 671 674 . . 674 677 + CviJI RGCY 675 675 . . 677 680 + FatI CATG 676 680 . . 677 680 + NlaIII CATG 680 676 . . 677 680 + CviAII CATG 677 679 . . 691 696 + VspI ATTAAT 692 694 . . 692 695 + MseI TTAA 692 694 . . 694 697 + TspEI AATT 693 697 . . 700 704 + Hpy188I TCNGA 702 701 . . 716 720 + Hpy188I TCNGA 718 717 . . 707 717 - Hin4I GAYNNNNNVTC 730 725 698 693 732 736 + SfaNI GCATC 741 745 . . 739 743 + DdeI CTNAG 739 742 . . 743 747 + TfiI GAWTC 743 746 . . 743 747 + HinfI GANTC 743 746 . . 757 762 + Hpy166II GTNNAC 759 759 . . 757 762 + AccI GTMKAC 758 760 . . 760 770 + Hin4I GAYNNNNNVTC 751 746 783 778 764 769 + TatI WGTACW 764 768 . . 764 769 + ScaI AGTACT 766 766 . . 765 768 + Csp6I GTAC 765 767 . . 765 768 + RsaI GTAC 766 766 . . 772 776 + BssKI CCNGG 771 776 . . 772 776 + BseBI CCWGG 773 774 . . 772 776 + ScrFI CCNGG 773 774 . . 772 776 + EcoRII CCWGG 771 776 . . 774 777 + SetI ASST 777 773 . . 770 775 - Eco57MI CTGRAG 755 753 . . 770 775 - GsuI CTGGAG 755 753 . . 775 779 + HphI GGTGA 787 786 . . 776 780 + Tsp45I GTSAC 775 780 . . 776 780 + MaeIII GTNAC 775 780 . . 782 787 + BclI TGATCA 782 786 . . 783 786 + DpnI GATC 784 784 . . 783 786 + BstKTI GATC 785 783 . . 783 786 + MboI GATC 782 786 . . 795 801 + TspRI CASTGNN 801 792 . . 804 809 + XbaI TCTAGA 804 808 . . 804 809 + Hpy178III* TCNNGA 805 807 . . 805 808 + MaeI CTAG 805 807 . . 808 812 + MboII GAAGA 820 819 . . 823 828 + NdeI CATATG 824 826 . . 828 832 - BslFI GGGAC 813 817 . . 835 839 + MboII GAAGA 847 846 . . 838 842 - SfaNI GCATC 828 832 . . 842 847 + Eco57MI CTGRAG 863 861 . . 842 847 + Eco57I CTGAAG 863 861 . . 844 848 + MboII GAAGA 856 855 . . 847 851 + MboII GAAGA 859 858 . . 851 854 + TspEI AATT 850 854 . . 855 859 + MboII GAAGA 867 866 . . 862 867 + PstI CTGCAG 866 862 . . 862 867 + SfeI* CTRYAG 862 866 . . 863 866 + CviRI* TGCA 864 864 . . 894 903 + MslI CAYNNNNRTG 898 898 . . 900 903 + FatI CATG 899 903 . . 900 903 + NlaIII CATG 903 899 . . 900 903 + CviAII CATG 900 902 . . 903 907 - BsmAI GTCTC 897 901 . . 933 944 + TstI CACNNNNNNTCC 924 919 956 951 933 939 + AarI CACCTGC 943 947 . . 934 937 + SetI ASST 937 933 . . 934 939 + BspMI ACCTGC 943 947 . . 932 936 - HphI GGTGA 924 923 . . 940 944 + BmgT120I GGNCC 941 942 . . 940 944 + AvaII GGWCC 940 943 . . 940 944 + AsuI* GGNCC 940 943 . . 938 941 - AciI CCGC 938 940 . . 942 947 + Hpy178III* TCNNGA 943 945 . . 947 951 + TspDTI ATGAA 962 960 . . 954 959 + Hpy178III* TCNNGA 955 957 . . 962 966 + BmgT120I GGNCC 963 964 . . 962 966 + AvaII GGWCC 962 965 . . 962 966 + AsuI* GGNCC 962 965 . . 965 969 + BccI CCATC 973 974 . . 968 972 + Hpy188I TCNGA 970 969 . . 971 975 + MboII GAAGA 983 982 . . 975 978 + SetI ASST 978 974 . . 971 976 - Ksp632I* CTCTTC 966 969 . . 974 977 - MnlI CCTC 967 966 . . 977 980 + Csp6I GTAC 977 979 . . 977 980 + RsaI GTAC 978 978 . . 979 989 + BsaXI ACNNNNNCTCC 969 966 999 996 984 987 + SetI ASST 987 983 . . 984 987 + CviJI RGCY 985 985 . . 984 987 + AluI AGCT 985 985 . . 985 995 + MwoI GCNNNNNNNGC 991 988 . . 993 996 + SetI ASST 996 992 . . 993 996 + CviJI RGCY 994 994 . . 993 996 + AluI AGCT 994 994 . . 994 998 + BlsI GCNGC 996 995 . . 994 998 + BisI GCNGC 995 996 . . 994 998 + TseI GCWGC 994 997 . . 994 998 - BbvI GCAGC 981 985 . . 995 1000 - EcoP15I CAGCAG 967 969 . . 1007 1011 - TspDTI ATGAA 997 995 . . 1018 1021 + MseI TTAA 1018 1020 . . 1021 1025 + TspDTI ATGAA 1036 1034 . . 1027 1030 + SetI ASST 1030 1026 . . 1038 1041 + CviJI RGCY 1039 1039 . . 1040 1045 + SmlI CTYRAG 1040 1044 . . 1040 1045 - Bce83I* CTTGAG 1025 1023 . . 1048 1052 + TspDTI ATGAA 1063 1061 . . 1057 1061 + MboII GAAGA 1069 1068 . . 1066 1071 + AsuII TTCGAA 1067 1069 . . 1067 1070 + TaqI TCGA 1067 1069 . . 1064 1068 - MboII GAAGA 1056 1055 . . 1063 1068 - BbvII* GAAGAC 1056 1060 . . 1081 1085 + TfiI GAWTC 1081 1084 . . 1081 1085 + HinfI GANTC 1081 1084 . . 1087 1097 + MwoI GCNNNNNNNGC 1093 1090 . . 1087 1097 + BglI GCCNNNNNGGC 1093 1090 . . 1088 1092 + BccI CCATC 1096 1097 . . 1095 1098 + CviJI RGCY 1096 1096 . . 1095 1098 + HaeIII GGCC 1096 1096 . . 1096 1100 + TauI GCSGC 1099 1096 . . 1096 1100 + BlsI GCNGC 1098 1097 . . 1096 1100 + BisI GCNGC 1097 1098 . . 1097 1102 + NspBII* CMGCKG 1099 1099 . . 1097 1100 + AciI CCGC 1097 1099 . . 1112 1115 + CviRI* TGCA 1113 1113 . . 1110 1114 - SfaNI GCATC 1100 1104 . . 1126 1134 + Tth111I GACNNNGTC 1129 1130 . . 1138 1142 + MboII GAAGA 1150 1149 . . 1138 1143 + BbvII* GAAGAC 1145 1149 . . 1142 1146 + Tsp4CI* ACNGT 1144 1143 . . 1144 1148 + HphI GGTGA 1156 1155 . . 1147 1152 + EcoRV GATATC 1149 1149 . . 1160 1164 + Hpy188I TCNGA 1162 1161 . . 1158 1163 - Eco57MI CTGRAG 1143 1141 . . 1158 1163 - Eco57I CTGAAG 1143 1141 . . 1164 1167 + SetI ASST 1167 1163 . . 1164 1167 + CviJI RGCY 1165 1165 . . 1164 1167 + AluI AGCT 1165 1165 . . 1180 1183 + TspEI AATT 1179 1183 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 242 943 # # AccI: # [GTMKAC] # 100 427 658 # # AciI: # [CCGC] # 88 159 938 # # AluI: # [AGCT] # 9 15 20 96 145 171 # 729 # # ApoI: # [RAATTY] # 472 713 # # AsuI*: # [GGNCC] # 22 223 940 # # AsuII: # [TTCGAA] # 118 1067 # # AvaII: # [GGWCC] # 22 223 940 # # BalI: # [TGGCCA] # 85 1100 # # BbvI: # [GCAGC] # 204 981 # # BbvII*: # [GAAGAC] # 40 89 241 815 # # BccI: # [CCATC] # 80 89 123 124 769 # # Bce83I*: # [CTTGAG] # 160 1025 # # BclI: # [TGATCA] # 403 782 # # BglI: # [GCCNNNNNGGC] # 92 1093 # # BinI*: # [GGATC] # 163 249 773 # # BisI: # [GCNGC] # 88 102 995 # # BlsI: # [GCNGC] # 87 102 996 # # BmgT120I: # [GGNCC] # 22 222 941 # # BsaXI: # [ACNNNNNCTCC] # 30 186 969 # # BseBI: # [CCWGG] # 50 152 412 571 # # BseYI: # [CCCAGC] # 141 1044 # # BsiYI*: # [CCNNNNNNNGG] # 405 780 # # BslFI: # [GGGAC] # 178 372 635 # # BsmAI: # [GTCTC] # 288 338 559 # # BspMI: # [ACCTGC] # 242 943 # # BsrI: # [ACTGG] # 421 764 # # BssKI: # [CCNGG] # 48 152 414 571 # # BstEII: # [GGTNACC] # 136 1049 # # BstKTI: # [GATC] # 74 171 175 365 400 # # Cfr10I: # [RCCGGY] # 67 1118 # # CfrI: # [YGGCCR] # 83 1102 # # Csp6I: # [GTAC] # 208 212 765 # # CspCI: # [CAANNNNNGTGG] # 35 501 649 # # CviAII: # [CATG] # 23 223 285 654 # # CviJI: # [RGCY] # 9 15 18 20 30 30 # 36 45 57 60 67 69 # 188 231 310 # # CviRI*: # [TGCA] # 72 249 864 # # DdeI: # [CTNAG] # 68 164 217 290 446 # # DpnI: # [GATC] # 74 170 175 365 401 # # Eco57I: # [CTGAAG] # 42 280 863 # # Eco57MI: # [CTGRAG] # 42 108 280 755 # # EcoP15I: # [CAGCAG] # 218 274 693 # # EcoRI: # [GAATTC] # 472 713 # # EcoRII: # [CCWGG] # 48 152 414 571 # # EcoRV: # [GATATC] # 36 1149 # # EspI*: # [GCTNAGC] # 514 671 # # FalI: # [AAGNNNNNCTT] # 0 0 32 88 1065 # # FatI: # [CATG] # 23 223 286 653 # # GsaI: # [CCCAGC] # 145 1040 # # GsuI: # [CTGGAG] # 430 755 # # HaeIII: # [GGCC] # 85 89 1011 # # HgiCI*: # [GGYRCC] # 295 890 # # Hin4I: # [GAYNNNNNVTC] # 21 32 32 402 698 # # Hin4II*: # [CCTTC] # 56 183 304 642 # # HindII: # [GTYRAC] # 520 665 # # HinfI: # [GANTC] # 104 338 352 391 # # HpaII: # [CCGG] # 68 1117 # # HphI: # [GGTGA] # 29 102 137 232 319 366 # # Hpy166II: # [GTNNAC] # 94 101 169 395 426 # # Hpy178III*: # [TCNNGA] # 12 138 172 230 255 378 # # Hpy188I: # [TCNGA] # 16 23 97 192 246 252 # 359 # # Ksp632I*: # [CTCTTC] # 219 966 # # MaeI: # [CTAG] # 122 216 380 467 # # MaeII: # [ACGT] # 12 401 772 # # MaeIII: # [GTNAC] # 136 273 366 410 # # MboI: # [GATC] # 74 168 175 365 403 # # MboII: # [GAAGA] # 3 8 9 13 27 34 # 35 59 73 81 116 153 # 255 319 # # MnlI: # [CCTC] # 14 90 112 218 295 456 # # MseI: # [TTAA] # 111 138 167 326 443 # # MslI: # [CAYNNNNRTG] # 287 898 # # MwoI: # [GCNNNNNNNGC] # 92 102 484 507 # # NdeI: # [CATATG] # 361 824 # # NlaIII: # [CATG] # 23 223 282 657 # # NlaIV: # [GGNNCC] # 297 888 # # NspBII*: # [CMGCKG] # 86 1099 # # PfoI: # [TCCNGGA] # 566 619 # # PstI: # [CTGCAG] # 319 866 # # RsaI: # [GTAC] # 207 212 766 # # ScaI: # [AGTACT] # 419 766 # # ScrFI: # [CCNGG] # 50 152 412 571 # # SetI: # [ASST] # 9 9 12 15 18 19 # 24 34 35 41 41 52 # 57 82 89 96 137 160 # 255 # # SfaNI: # [GCATC] # 85 87 272 366 375 # # SfeI*: # [CTRYAG] # 323 862 # # SmlI: # [CTYRAG] # 145 1040 # # TaiI: # [ACGT] # 12 404 769 # # TaqI: # [TCGA] # 20 30 118 330 687 # # TatI: # [WGTACW] # 421 764 # # TauI: # [GCSGC] # 86 1099 # # TfiI: # [GAWTC] # 104 338 352 391 # # TseI: # [GCWGC] # 191 994 # # TsoI: # [TARCCA] # 460 725 # # Tsp45I: # [GTSAC] # 366 409 410 # # Tsp4CI*: # [ACNGT] # 41 267 877 # # TspDTI: # [ATGAA] # 27 35 39 122 962 # # TspEI: # [AATT] # 6 35 45 52 58 111 # 157 329 392 # # TspRI: # [CASTGNN] # 384 801 # # TstI: # [CACNNNNNNTCC] # 32 32 111 229 781 # # Tth111I: # [GACNNNGTC] # 56 1129 # # VspI: # [ATTAAT] # 493 692 # # XbaI: # [TCTAGA] # 381 804 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1185 # Reported_sequences: 1 # Reported_hitcount: 254 #---------------------------------------